WO2005023004A1 - Composition de pesticide microbien - Google Patents

Composition de pesticide microbien Download PDF

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Publication number
WO2005023004A1
WO2005023004A1 PCT/JP2004/011532 JP2004011532W WO2005023004A1 WO 2005023004 A1 WO2005023004 A1 WO 2005023004A1 JP 2004011532 W JP2004011532 W JP 2004011532W WO 2005023004 A1 WO2005023004 A1 WO 2005023004A1
Authority
WO
WIPO (PCT)
Prior art keywords
pesticide composition
microbial pesticide
microorganism
oxygen
inorganic salt
Prior art date
Application number
PCT/JP2004/011532
Other languages
English (en)
Japanese (ja)
Inventor
Akihiko Narutaki
Original Assignee
Idemitsu Kosan Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idemitsu Kosan Co., Ltd. filed Critical Idemitsu Kosan Co., Ltd.
Publication of WO2005023004A1 publication Critical patent/WO2005023004A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof

Definitions

  • the present invention relates to a microbial pesticide composition, and more particularly, to a microbial pesticide composition in which drug spots on a crop surface caused by spraying the microbial pesticide composition are reduced.
  • microbial pesticides using microorganisms that antagonize pathogenic bacteria causing various diseases have been widely used in consideration of safety and sustainability of the effects.
  • a microbial pesticide uses a water-insoluble extender, such as a clay mineral, in order to prevent uneven distribution of an active ingredient in a composition and to improve storage stability.
  • Patent Documents 1 and 2 disclose microbial pesticides using talc as a bulking agent.
  • Patent Document 1 JP-A-8-175919
  • Patent Document 2 JP-A-8-175920
  • the present invention has been made in view of the above, and has been developed by spraying a microbial pesticide composition. It is an object of the present invention to provide a microbial pesticide composition in which drug spots on the surface of a product are reduced and which have a sufficient plant disease control effect.
  • the present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have achieved the above object by using a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea.
  • the inventors have found that the present invention has been completed, and based on these findings, have completed the present invention.
  • the gist of the present invention is as follows.
  • a microbial pesticide composition containing a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea is a microbial pesticide composition containing a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea.
  • the content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urine, and the content of the urea is The microbial pesticide composition according to (1), wherein the amount is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea.
  • a microbial pesticide composition comprising a microorganism having a plant disease controlling effect, an oxygen-containing inorganic salt, urea, and a surfactant.
  • the content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urine, and the content of the urea is The total amount of the oxygen-containing inorganic salt and the urea is 10 to 50% by weight, and the content of the surfactant is 530% by weight based on the whole microbial pesticide composition.
  • the microbial pesticide composition according to (4).
  • micro-organism according to (4) or (5) wherein the content of the microorganism is 1030% by weight based on the total weight of the microorganism, the oxygen-containing inorganic salt, and the urea. Biopesticide composition.
  • the oxygen-containing inorganic salt is a carbonate or a sulfate.
  • the microbial pesticide composition of the present invention is a microbial pesticide composition containing a microorganism having a plant disease controlling effect, an oxygen-containing inorganic salt and urea.
  • microorganism used in the present invention is not particularly limited as long as it is a microorganism having an effect of controlling plant diseases.
  • microorganism having an effect of controlling plant diseases refers to preventing the occurrence of a certain plant disease or treating a plant disease by treating the plant with the microorganism, such as spraying the microorganism on the plant.
  • a microorganism that has the ability to heal bacteria is not particularly limited as long as it is a microorganism having an effect of controlling plant diseases.
  • microorganisms examples include Bacillus bacteria, Dariocladium bacteria, Trichoderma bacteria, Talalomyces bacteria, Penicillium bacteria and the like.
  • Bacillus bacteria are effective in controlling, for example, gray mold, powdery mildew, and mildew, Daliocladium bacteria are effective in controlling gray mold, etc.
  • Trichoderma bacteria are effective in controlling gray mold. It is effective in controlling disease and the like, Talalomyces bacteria are effective in controlling powdery mildew and anthracnose, and Penicillium bacteria are effective in controlling anthracnose and the like. Since it exerts a controlling effect on various plant diseases, it can be used as a microorganism in the present invention.
  • Bacillus subtilis which is preferred by Bacillus bacteria.
  • Bacillus subtilis includes, for example, strains NCIB12376 and NCIB12616.
  • NCIB12376 and NCIB12616 strains are available from the National Collections of Industrial, Food and Marine Bacteria (NCIMB).
  • the microorganism used in the present invention may be used alone or in combination of two or more.
  • microorganism used in the present invention for example, a microorganism contained in a commercially available viable agent or the like, or a microorganism cultured using a commercially available bacterial strain may be used.
  • the method for culturing the microorganism used in the present invention is not particularly limited, and can be performed by a conventional method.
  • the cells can be cultured in a medium in which microorganisms can grow, and the cells can be collected by means such as centrifugation.
  • the microorganism used in the present invention is a live microorganism.
  • the microorganisms used in the present invention may not be spores, but are preferably spores from the viewpoint of preservability of the microbial pesticide composition as a product. Therefore, in order to form spores, at the end of the culture, the culture conditions such as the composition of the medium, the pH of the medium, the culture temperature, the culture humidity, and the oxygen concentration during culturing are adjusted to match the spore formation conditions. Is preferred.
  • the water content of the spores is preferably 20% by weight or less from the viewpoint of the preservability of the microbial pesticide composition.
  • the amount of microorganisms contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but is 5 to 50% based on the total amount of microorganisms, oxygen-containing inorganic salts and urea. %, Preferably from 10 to 30% by weight (dry weight).
  • the concentration of microorganisms contained in the microbial pesticide composition of the present invention is preferably 1 ⁇ 10 5 1 ⁇ 10 15 cfti / g, more preferably 1 ⁇ 10 9 —1 ⁇ 10 12 It can be cfu / g.
  • the oxygen-containing inorganic salt used in the present invention is not particularly limited as long as the effects of the present invention are not hindered.
  • examples include carbonates, sulfates, phosphates, and nitrates.
  • Sodium hydrogen carbonate is particularly preferred as a carbonate, and sodium sulfate is particularly preferred as a sulfate.
  • the amount of the oxygen-containing inorganic salt contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired. Preferably it is 50% by weight, more preferably 20-45% by weight.
  • oxygen-containing inorganic salt is contained in these ranges, from the viewpoint of controlling effect on plant diseases, hydration of the microbial pesticide composition, and reduction of spots on the crop surface caused by spraying the microbial pesticide composition, This is because a microbial pesticide composition with a good balance can be obtained.
  • the amount of urea contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but is 1050% by weight based on the total amount of microorganisms, oxygen-containing inorganic salts and urea. 20% to 45% by weight is more preferable.
  • urea is contained in these ranges, a well-balanced microorganism can be obtained from the viewpoint of controlling plant diseases, hydrating the microbial pesticide composition, and reducing the spots on the crop surface caused by the application of the microbial pesticide composition. This is because an agricultural chemical composition can be obtained.
  • the microbial pesticide composition of the present invention may not contain a surfactant, but preferably contains a surfactant. This is because when a surfactant is contained, the dispersibility and suspension of the microorganism, which is an active ingredient, in the spray liquid are improved when the spray liquid is prepared by diluting the microbial pesticide composition.
  • the surfactant used in the present invention may be any type of surfactant, such as an anion type and a cationic type, which is not particularly limited, as long as the effects of the present invention are not hindered, but SOLPOL5082 ( Anionic surfactants such as Toho Chemical Co., Ltd.) are preferred.
  • the amount of the surfactant to be contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but it is preferably 5 to 30% by weight based on the total amount of the microbial pesticide composition. Preferably, the content is more preferably 8 to 20% by weight.
  • the surfactant is contained in these ranges, the hydration property of the microorganisms is improved, the susceptibility of the microorganisms is improved, and the preservation of the microbial pesticide composition is sufficiently ensured. This is because almost no phytotoxicity occurs.
  • the microbial pesticide composition of the present invention may contain, in addition to the above-mentioned substances, a water-soluble substance, for example, as long as it does not hinder the effects of the present invention.
  • the dosage form of the microbial pesticide composition of the present invention is not particularly limited, and may be granular, powdery, or liquid. Etc., but are preferably in the form of granules or powder.
  • the average particle diameter is more preferably in the range of 1 ⁇ -100 ⁇ , and more preferably in the range of 1 ⁇ -50 / im.
  • the microbial pesticide composition is easy to handle, and when the average particle size is lxm or less, the microbial spore diameter may be smaller than the microbial spore diameter. This is because there is a case where the plant is killed and the effect of controlling plant diseases is not sufficiently exhibited.
  • the microbial pesticide composition of the present invention can be used for controlling plant diseases such as gray mold and petal gray mold.
  • the method for using the microbial pesticide composition of the present invention is not particularly limited, but may be diluted with water so as to have a predetermined dilution ratio determined at the time of pesticide registration, for example, 1,000 times, 2000 times, 4000 times, and the like.
  • a spray liquid can be prepared, and the spray liquid can be used, for example, by spraying the whole plant in a mist state using a power sprayer or the like.
  • Bouillon medium of 100 ml (meat extract 1% by weight, peptone 1%, sodium chloride 0.5 by weight 0/0) are prepared three Sakaguchi flask 500ml flasks containing, respectively, Bacillus' Zubuchi squirrel NCIB12376 strain After inoculating one platinum loop of the slant culture of the stock bacterium, the flask was cultured at 30 ° C. for 1 day while shaking the flask at 10 cm amplitude and 120 rpm using a reciprocating shaker.
  • a 30-liter fermenter containing a 15-liter medium (glucose 2% by weight, polypeptone 1% by weight, yeast extract 0.2% i%, KHPO 0.1% by weight) was prepared, and the culture obtained above was prepared.
  • a total of 300 ml of the product was inoculated into the fermenter and cultured under aerobic conditions at 30 ° C. for 72 hours to obtain a culture solution.
  • About 15 L of the obtained culture solution was centrifuged (6000 rpm, 20 minutes) according to a standard method to separate into a culture supernatant and a cell precipitate.
  • the cell precipitate was washed with water to obtain a wet cell (spore fraction) with a wet weight of about 780 g.
  • This spore fraction and have you in a dry state, are those containing 100 parts by weight 0/0 spores of Bacillus' subtilis NCIB12376 shares (Production Example 2)
  • Bacillus subtilis NCIB12376 instead of using Bacillus subtilis NCIB12376, the same operation as in Production Example 1 was performed using Bacillus subtilis NCIB12616 to obtain a spore fraction having a wet weight of about 750 g. This spore fraction contains 50% by weight of spores of Bacillus subtilis NCIB 12616 in a dry state.
  • Example 1 of JP-A-8-175920 was prepared, and this was used as Comparative Example 6, and the number of bacteria in the composition was similarly measured over time. Table 2 shows the results.
  • a 500 ml beaker containing 200 ml of the test water was prepared, and a positioning force of about 10 cm above the water surface in the beaker was sprayed so that 5 g of the pesticidal composition of the present invention was spread over the whole water surface.
  • the time from when the pesticide composition was sprayed on the water surface until it sank to the water floor was measured and used as an index of hydration.
  • Table 3 shows the results of this hydration test.
  • the control effect on the eggplant gray mold was tested in a vinyl greenhouse (variety: Senryo No. 2, 5 test plots / division, 3 replicates). Inoculation of eggplant gray mold was carried out by hanging eggplant fruits cultivated by infection with gray mold and hanging them in a greenhouse.
  • the microbial pesticide composition was formulated in the form of eggplant flowering, fruiting season, diluted with water in Formulation Example 1 (2000-fold dilution), Formulation Example 2 (1000-fold dilution), Formulation Example 3 (1000-fold dilution), Formulation Example 4 (2000-fold dilution), Comparative Example 1 (1000-fold dilution), and Comparative Example 6 (1000-fold dilution) were each sprayed at 7-day intervals.
  • Spraying was performed by spraying a diluted solution of each pesticide composition on the entire plant at a rate of 300 L per 10a. Seven days after the third application, each strain was examined, and the diseased flower rate was calculated. The results are shown in Table 4.In addition, the same test was conducted without spraying the formulation example and the comparative example, and the flowering rate of the diseased fruit was calculated, and the control value of the formulation example and the comparative example was determined based on this value. Calculated.
  • Table 5 From the results in Table 5, it was confirmed that the microbial pesticide composition of the present invention has a sufficient control effect against plant diseases.
  • the microbial pesticide composition of the present invention it was examined whether or not drug spots on the crop surface caused by the application of the microbial pesticide composition were actually reduced.
  • Table 6 1 No stain due to spots is observed.
  • the microbial pesticide composition which reduces the drug spot on the crop surface which arises by spraying a microbial pesticide composition, and has a sufficient plant disease control effect is provided.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne une composition de pesticide microbien contenant un micro-organisme ayant un effet de suppression des lésions pathologiques sur les plantes, un sel inorganique contenant de l'oxygène et de l'urée, au moyen desquels la formation de taches sur la surface de la récolte, induite par l'application de composition de pesticide microbienne, est supprimée et un effet suffisant de suppression de lésion pathologique sur les plantes peut être obtenu.
PCT/JP2004/011532 2003-09-03 2004-08-11 Composition de pesticide microbien WO2005023004A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003311582A JP2005075813A (ja) 2003-09-03 2003-09-03 微生物農薬組成物
JP2003-311582 2003-09-03

Publications (1)

Publication Number Publication Date
WO2005023004A1 true WO2005023004A1 (fr) 2005-03-17

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ID=34269704

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Application Number Title Priority Date Filing Date
PCT/JP2004/011532 WO2005023004A1 (fr) 2003-09-03 2004-08-11 Composition de pesticide microbien

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JP (1) JP2005075813A (fr)
KR (1) KR20060125696A (fr)
CN (1) CN1845677A (fr)
TW (1) TW200511932A (fr)
WO (1) WO2005023004A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08175921A (ja) * 1994-12-22 1996-07-09 Idemitsu Kosan Co Ltd 農園芸用殺菌剤組成物
JPH08175919A (ja) * 1994-12-20 1996-07-09 Idemitsu Kosan Co Ltd バチルス属胞子画分及びその胞子画分を利用する植物病害防除法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08175919A (ja) * 1994-12-20 1996-07-09 Idemitsu Kosan Co Ltd バチルス属胞子画分及びその胞子画分を利用する植物病害防除法
JPH08175921A (ja) * 1994-12-22 1996-07-09 Idemitsu Kosan Co Ltd 農園芸用殺菌剤組成物

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Publication number Publication date
CN1845677A (zh) 2006-10-11
JP2005075813A (ja) 2005-03-24
TW200511932A (en) 2005-04-01
KR20060125696A (ko) 2006-12-06

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