CN1845677A - Microbial pesticide composition - Google Patents
Microbial pesticide composition Download PDFInfo
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- CN1845677A CN1845677A CNA2004800253777A CN200480025377A CN1845677A CN 1845677 A CN1845677 A CN 1845677A CN A2004800253777 A CNA2004800253777 A CN A2004800253777A CN 200480025377 A CN200480025377 A CN 200480025377A CN 1845677 A CN1845677 A CN 1845677A
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- 239000000203 mixture Substances 0.000 title claims abstract description 115
- 239000000575 pesticide Substances 0.000 title claims abstract description 89
- 230000000813 microbial effect Effects 0.000 title claims abstract description 87
- 244000005700 microbiome Species 0.000 claims abstract description 53
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000004202 carbamide Substances 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 26
- 201000010099 disease Diseases 0.000 claims abstract description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 31
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 31
- 235000010755 mineral Nutrition 0.000 claims description 31
- 239000011707 mineral Substances 0.000 claims description 31
- 239000001301 oxygen Substances 0.000 claims description 31
- 229910052760 oxygen Inorganic materials 0.000 claims description 31
- 150000003839 salts Chemical class 0.000 claims description 30
- 239000004094 surface-active agent Substances 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical group [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 7
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 7
- 229910021653 sulphate ion Inorganic materials 0.000 claims description 7
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical group [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 6
- 239000003945 anionic surfactant Substances 0.000 claims description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 3
- 235000011152 sodium sulphate Nutrition 0.000 claims description 3
- 238000003892 spreading Methods 0.000 abstract description 24
- 125000004334 oxygen containing inorganic group Chemical group 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 24
- 230000000052 comparative effect Effects 0.000 description 20
- 241000894006 Bacteria Species 0.000 description 19
- 238000009472 formulation Methods 0.000 description 18
- 239000003814 drug Substances 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 14
- 238000007865 diluting Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 9
- 244000061458 Solanum melongena Species 0.000 description 8
- 235000002597 Solanum melongena Nutrition 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 239000003905 agrochemical Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003595 mist Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000896533 Gliocladium Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000001549 Ipomoea eriocarpa Species 0.000 description 2
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 description 2
- 241000228143 Penicillium Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 235000012222 talc Nutrition 0.000 description 2
- 230000036962 time dependent Effects 0.000 description 2
- YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- FHKPLLOSJHHKNU-INIZCTEOSA-N [(3S)-3-[8-(1-ethyl-5-methylpyrazol-4-yl)-9-methylpurin-6-yl]oxypyrrolidin-1-yl]-(oxan-4-yl)methanone Chemical compound C(C)N1N=CC(=C1C)C=1N(C2=NC=NC(=C2N=1)O[C@@H]1CN(CC1)C(=O)C1CCOCC1)C FHKPLLOSJHHKNU-INIZCTEOSA-N 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- PANBYUAFMMOFOV-UHFFFAOYSA-N sodium;sulfuric acid Chemical compound [Na].OS(O)(=O)=O PANBYUAFMMOFOV-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a microbial pesticide composition which contains a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt and urea, reduces the occurrence of surface spots on crops caused by spreading the microbial pesticide composition, and has a sufficient effect of controlling plant diseases.
Description
Technical field
The present invention relates to microbial pesticide composition, relate to the microbial pesticide composition of the crop surface medicine spot that minimizing produces by the spreading microbial pesticide composition in more detail.
Background technology
In recent years, be not subjected to the method for various diseases, consider the continuation of safety, drug effect, use anti-ly to cause that the microbial pesticide of various disease pathogen microorganisms has obtained applying as cover crop.Usually, in the microbial pesticide,, use water-fast incremental agents such as clay mineral in order to prevent the active ingredient skewness in the composition and to improve bin stability.For example in patent documentation 1 and patent documentation 2, disclose and used the microbial pesticide of talcum as incremental agent.But containing in a large number under the situations of insoluble mineral as the microbial pesticide composition of incremental agent such as talcum, in the process of spreading microbial pesticide, incremental agent micro mist in the composition generates the medicine spot attached to crop surface on crop, exist the problem that reduces the crop commodity value.
Medicine spot when preventing the spreading agricultural chemicals, considered from microbial pesticide composition, to remove the method for insoluble incremental agent, but in this case, the microbial profile of active ingredient is inhomogeneous, it is inhomogeneous that composition also becomes, when making it be dissolved in the spreading liquid simultaneously, has the dispersed bad shortcoming of microbial pesticide composition.Also consider the method for incremental agent being changed into water-soluble substances on the other hand, but some incremental agent exists the microbial pesticide composition effect is reduced, or make the problems such as bin stability reduction of microbial pesticide composition.
Therefore wish to have a kind of energy to reduce the crop surface medicine spot that produces owing to the spreading microbial pesticide composition, and the microbial pesticide composition with abundant controlling plant diseases effect.
Patent documentation 1: the spy opens flat 8-175919 communique
Patent documentation 2: the spy opens flat 8-175920 communique
Disclosure of the Invention
The present invention studies according to above-mentioned viewpoint, and purpose is to provide and can reduces the crop surface medicine spot that is produced by the spreading microbial pesticide composition, and the microbial pesticide composition with abundant controlling plant diseases effect.
In order to solve above-mentioned problem, the present inventor has carried out research with keen determination repeatedly, found that by use have the controlling plant diseases effect microorganism, contain oxygen mineral salt and urea, can achieve the above object, finished the present invention based on these viewpoints.
Main main points just of the present invention are as follows:
(1) microbial pesticide composition, it contains microorganism with controlling plant diseases effect, contains oxygen mineral salt and urea.
(2) according to the microbial pesticide composition described in (1), wherein, the aforementioned oxygen mineral salt that contain are 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, and aforementioned urea is 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
(3) according to the microbial pesticide composition described in (1) or (2), wherein, aforementioned micro organism is 10～30 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
(4) microbial pesticide composition, it contains microorganism with controlling plant diseases effect, contains oxygen mineral salt, urea and surfactant.
(5) according to the microbial pesticide composition described in (4), wherein, the aforementioned oxygen mineral salt that contain are 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, aforementioned urea is 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, and aforementioned surfactants is 5～30 weight % with respect to the content of aforementioned micro organism composition pesticide total amount.
(6) according to the microbial pesticide composition described in (4) or (5), wherein, aforementioned micro organism is 10～30 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
(7) according to the microbial pesticide composition described in any one of (4)～(6), wherein, aforementioned surfactants is an anionic surfactant.
(8) according to the microbial pesticide composition described in any one of claim (1)～(7), wherein, the aforementioned oxygen mineral salt that contain are carbonate or sulphate.
(9) according to the microbial pesticide composition described in (8), wherein, aforementioned carbonate is sodium bicarbonate.
(10) according to the microbial pesticide composition described in (8) or (9), wherein, aforementioned sulphate is sodium sulphate.
(11) according to the microbial pesticide composition described in any one of (1)～(10), wherein, aforementioned micro organism is a bacillus.
(12) according to the microbial pesticide composition described in (11), wherein, aforementioned bacillus is a bacillus subtilis.
(13) according to the microbial pesticide composition described in (11), wherein, aforementioned bacillus is NCIB12376 strain or NCIB12616 strain.
Implement preferred plan of the present invention
Below describe the present invention in detail.
Microbial pesticide composition of the present invention is to comprise the microorganism with controlling plant diseases effect, the microbial pesticide composition that contains oxygen mineral salt and urea.
Used microorganism among the present invention so long as have the microorganism of controlling plant diseases effect, then is not particularly limited.Said among the present invention " microorganism with controlling plant diseases effect " is meant by microorganism is sprayed at plant first-class, with this microorganism plant handled, and has to prevent certain plant disease, perhaps cures the microorganism of plant disease ability.
As such microorganism, for example bacillus, Gliocladium bacterium, trichoderma bacterium, ラ ロ マ イ セ ス (tararomyces) belong to bacterium, Penicillium bacterium etc.Bacillus is effective as gray mold, mildew and black points etc. for control, the Gliocladium bacterium is for preventing and treating gray mold etc. effectively, the trichoderma bacterium is for preventing and treating gray mold etc. effectively, it is effective for preventing and treating mildew and anthracnose etc. that ラ ロ マ イ セ ス (tararomyces) belongs to bacterium, and the Penicillium bacterium is for preventing and treating anthracnose etc. effectively.Consider from the effect of the various plant diseases of performance control,, preferably use the bacterium of bacillus, more preferably use bacillus subtilis as being used for microorganism of the present invention.As such bacillus subtilis, can enumerate NCIB12376 strain and NCIB12616 strain.NCIB12376 strain and NCIB12616 strain can be from NationalCollections of Industrial, and Food and Marine Bacteria (NCIMB) buys.
Used microorganism among the present invention can be used alone, and also can use several simultaneously.
Used microorganism among the present invention both can have been used contained microorganism in live bacteria agent of selling on the market etc., the microorganism that also can use the strain culturing of selling on the market.
Method for cultivating microorganism used therefor of the present invention is not particularly limited, and can use conventional method to cultivate.The medium that for example uses microorganism to grow is cultivated, and uses method such as centrifugation to reclaim thalline.
The microorganism that the present invention is used is the viable bacteria of microorganism.Being used for microorganism live bacteria of the present invention simultaneously, can not be spore, but from considering as the viewpoint of microbial pesticide composition goods storage property, preferably uses spore.Therefore in order to form spore, the final stage cultivating preferably is adjusted to the condition that is suitable for forming this spore to the condition of culture such as oxygen concentration in the pH of the composition of medium, medium, cultivation temperature, cultivation humidity, the incubation.In addition, when using the spore of microorganism in the present invention, store the viewpoint of property from microbial pesticide composition and consider, preferably the water content of spore is controlled at 20 weight % or below the 20 weight %.
About microbial biomass contained in the microbial pesticide composition of the present invention,, then be not particularly limited as long as without detriment to effect of the present invention.Its with respect to microorganism, contain the oxygen mineral salt and the urea total amount is preferably 5～50 weight % (dry weight), more preferably 10～30 weight %.The concentration of contained microorganism in the microbial pesticide composition of the present invention simultaneously, as the unit that forms bacterium colony, can preferred 1 * 10
5～1 * 10
15Cfu/g, more preferably 1 * 10
9～1 * 10
12Cfu/g.
As the employed oxygen mineral salt that contain among the present invention, as long as do not hinder effect of the present invention, then be not particularly limited, for example can enumerate carbonate, sulphate, phosphate and nitrate etc.Preferred carbonate and sulphate, as carbonate, preferred especially sodium bicarbonate; As the special preferably sulfuric acid sodium of sulphate.
The contained amount that contains the oxygen mineral salt in the microbial pesticide composition of the present invention, as long as without detriment to effect of the present invention, then be not particularly limited, it is preferably 10～50 weight % with respect to microorganism, the total amount that contains oxygen mineral salt and urea, more preferably 20～45 weight %.This is owing to consider from the effect of controlling plant diseases, the hydrability of microbial pesticide composition and the viewpoint of the crop surface medicine spot that minimizing spreading microbial pesticide composition is produced, contain the oxygen mineral salt if contain, just can obtain the microbial pesticide composition of good combination property in these scopes.
As long as the amount of contained urea in the microbial pesticide composition of the present invention without detriment to effect of the present invention, then is not particularly limited, and it is preferably 10～50 weight % with respect to microorganism, the total amount that contains oxygen mineral salt and urea, more preferably 20～45 weight %.This is owing to consider from the effect of controlling plant diseases, the hydrability of microbial pesticide composition and the viewpoint of the crop surface medicine spot that minimizing spreading microbial pesticide composition is produced, if contain urea, just can obtain the microbial pesticide composition of good combination property in these scopes.
Microbial pesticide composition of the present invention in addition can not contain surfactant, also can contain surfactant.Because if make it contain surfactant, just can when microbial pesticide composition being diluted preparation spreading liquid, improve dispersiveness and the drapability of effective ingredient microorganism in spreading liquid.As surfactant used among the present invention, so long as do not hinder effect of the present invention, then be not particularly limited, can be anionic, unsolicited surfactant such as cationic.Preferred SOLPOL5082 anionic surfactant such as (eastern nation chemistry (strain) systems).
As long as the amount of the surfactant that is contained in the microbial pesticide composition of the present invention without detriment to effect of the present invention, then is not particularly limited.Preferred its total amount with respect to microbial pesticide composition is 5～30 weight %, more preferably 8～20 weight %.Because when when this scope contains surfactant, can improve the hydrability drapability of microorganism, fully guarantee the storage of microbial pesticide composition, can produce hardly owing to the spreading microbial pesticide composition causes the infringement of agricultural chemicals to plant simultaneously.
Microbial pesticide composition of the present invention except containing above-mentioned substance, can also contain the material that does not hinder effect of the present invention, for example water-soluble substances etc.
Agent shape as microbial pesticide composition of the present invention is not particularly limited, and can be granular, powdery, aqueous etc., preferably granular, powdery.At this moment average grain diameter is preferably in 1 μ m～100 mu m ranges, more preferably in 1 μ m～50 mu m ranges.Average grain diameter is in this scope the time, the use processing ease of microbial pesticide composition, in addition because when its particle diameter when 1 μ m or 1 μ m are following, the diameter owing to microbial spore reduces sometimes, microorganism can be dead, so can not give full play to the control efficiency to plant disease.
Microbial pesticide composition of the present invention can be used for preventing and treating the disease of plants such as grey mould of egg plant and petal gray mold.
Using method about microbial pesticide composition of the present invention, be not particularly limited, the extension rate of defined when for example can water it being diluted to registration agricultural chemicals such as 1000 times, 2000 times, 4000 times, preparation spreading liquid, working power sprayers etc. spray this vaporific spreading liquid to whole plants, method such as handle.
Further the present invention is specifically described by the following examples.
Embodiment
＜embodiment 1 〉
The manufacturing of the wet thallus (spore component) of (Production Example 1) bacillus
Prepare the slope mouth flask that 3 addings have the 500ml volume of 100ml bacteria culture media (meat extract 1 weight %, peptone 1 weight %, sodium chloride 0.5 weight %), the slant culture of bacterial strain is preserved in the bacillus subtilis NCIB12376 strain that inserts a platinum loop respectively, use the double vibrations machine with amplitude: 10cm then, revolution: 120rpm cultivated one day on 30 ℃ of following vibration flask limits.
Prepare an adding on the other hand 15L medium (glucose 2 weight %, polypepton 1 weight %, yeast extract 0.2 weight %, KH are arranged
2PO
40.1 the fermentation tank of 30L volume weight %) meets the culture total 300ml that bacterium obtains previously in this fermentation tank, under aerobic condition, in 30 ℃ of cultivations 72 hours, obtain culture fluid.According to conventional method about 15L culture fluid of gained is carried out centrifugation (6000rpm, 20 minutes) and be separated into culture supernatant and bacterial sediment thing.After removing culture supernatant, water cleans the bacterial sediment thing, obtains the wet thallus (spore component) that wet weight is about 780g, and this spore component is the spore component that contains 100 weight % bacillus subtilis NCIB12376 strain spores under drying regime.
(Production Example 2)
Use bacillus subtilis NCIB12616 strain to substitute bacillus subtilis NCIB12376 strain, carry out and above-mentioned Production Example 1 identical operations, obtain the spore component that wet weight is about 750g, this spore component, under drying regime, contain 50 weight % bacillus subtilis NCIB12616 strains.
Spore makes powder (Production Example 3)
The about 600g of spore component that obtains bacillus subtilis NCIB12376 strain by above-mentioned Production Example 1 is suspended among the water 300L, uses spray dryer, handle (inlet temperature is 150 ℃, and outlet temperature is 100 ℃) with the speed of 1.5～2.0L/h.The dry thing that spray-dried machine obtains is pulverized, obtained the spore powder that dry weight is about 78g.
(Production Example 4)
The about 600g of spore component that obtains with Production Example 2 is carried out the processing identical with above-mentioned Production Example 3, obtain the spore powder of about 78g bacillus subtilis NCIB12616 strain.
(Production Example 5)
Use the spore powder of the bacillus subtilis NCIB12376 strain that above-mentioned Production Example 3 obtains, material with record in the composition his-and-hers watches 1 of record in the table 1 mixes, use the jet mill cracker carry out micro mist broken after, fully mix with the crawler type mixer, obtain formulation example 1,2 and 3 and comparative example 1,2,3,4 and 5.
Use the spore powder of the bacillus subtilis NCIB12616 strain that above-mentioned Production Example 4 obtains, material with record in the composition his-and-hers watches 1 of record in the table 1 mixes, use the jet mill cracker carry out micro mist broken after, fully mix with the crawler type mixer, obtain formulation example 4.
As surfactant, used the SOLPOL5082 (eastern nation chemistry (strain) system) of anionic surfactant.
Table 1
| Formulation example | Comparative example | ||||||||
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 5 | |
| Microbial spore (wt%) | 24 | 12 | 12 | 24 | 12 | 12 | 12 | 12 | 12 |
| Sodium bicarbonate (wt%) | 39 | 39 | 78 | ||||||
| Sodium sulphate (wt%) | 33 | 39 | 39 | 33 | 39 | 78 | |||
| Calcium chloride (wt%) | 78 | ||||||||
| Urea (wt%) | 33 | 39 | 33 | 78 | |||||
| Surfactant (wt%) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Add up to (wt%) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
＜embodiment 2 〉
For the bin stability with the microbial pesticide composition of Production Example manufacturing is studied, measured the time dependent situation of bacterium number in the composition.And make the special composition of opening explanation among the flat 8-175920 communique embodiment 1, with this as a comparative example 6, measured the time dependent situation of bacterium number in the composition equally, measurement result is shown in table 2.
Table 2
Shown in table 2 is clear and definite, compare the bin stability excellence of microbial pesticide composition of the present invention with comparative example.
＜embodiment 3 〉
Whether composition pesticide of the present invention for spreading uses with water-reducible composition, is fully studied the hydrability of microbial pesticide composition of the present invention.Measure hydrability in accordance with the following methods.
Calcium carbonate 0.3077g and magnesium carbonate 0.092g are dissolved in a spot of I standard watery hydrochloric acid.In sand bath resulting solution being carried out evaporation drying solidifies.Remaining solid constituent is dissolved among the desalted water 10L test water of using as soluble test.The volume that then preparing an adding has this test water 200ml is the beaker of 500ml, from the position spreading that is higher than the about 10cm of the water surface in beaker composition pesticide 5g of the present invention, makes it expand to the whole water surface then.Mensuration is deposited to the water-bed time to it after composition pesticide is dispensed onto the water surface, as the hydrability index.
The result of this hydrability test is shown in table 3.
Table 3
| Hydrability (second) | |
| Formulation example 1 | 9 |
| Formulation example 2 | 8 |
| Formulation example 3 | 9 |
| Formulation example 4 | 9 |
| Comparative example 1 | 5 |
| Comparative example 2 | 6 |
| Comparative example 3 | 5 |
| Comparative example 4 | 10 |
| Comparative example 5 | 9 |
＜embodiment 4 〉
Whether can bring into play sufficient control efficiency to plant disease to microbial pesticide composition of the present invention studies.
(1) test of control grey mould of egg plant
In plastic green house, carried out testing (kind: 1,002 No. 2,5 strain/districts, test region repeat 3 times) for the effect of control grey mould of egg plant.The inoculation of grey mould of egg plant is the eggplant fruit that infects the ash arrhizus bacteria cultivation to be suspended in the greenhouse carry out.The while microbial pesticide composition, in the blooming of eggplant, fruiting period, formulation example 1 (diluting 2000 times) every 7 days difference spreading dilute with waters, formulation example 2 (diluting 1000 times), formulation example 3 (diluting 1000 times), formulation example 4 (diluting 2000 times), comparative example 1 (diluting 1000 times), comparative example 6 (diluting 1000 times).Spreading is to be that the dilution that each composition pesticide is handled in 300L spraying carries out to whole plant with every 10a., after 7 days each plant is studied since the 3rd spreading, calculated morbidity fruit flower rate.The result is shown in table 4.
Simultaneously with spreading formulation example neither, also the method for spreading comparative example is not tested equally, calculating morbidity fruit flower rate, as standard, calculates the control value of formulation example and comparative example with this numerical value.
Table 4
(2) test of control petal gray mold
Use the morning glory petal, research formulation example and comparative example are to the effect of gray mold.The inoculation of gray mold is by spraying contains 1 * 10 to petal
5The cfu/g gray mold pathogen aqueous solution carries out.The formulation example 1 (dilute 2000 times) of spraying dilute with water on such petal, comparative example 1 (diluting 1000 times), comparative example 6 (diluting 1000 times) is handled, under 20 ℃ through one day after, study the situation of morning glory petal.The result is shown in table 5.
Table 5
| Occurring degree | |
| Formulation example 1 (diluting 2000 times) | -(not morbidity) |
| Comparative example 6 (diluting 1000 times) | -(not morbidity) |
| Comparative example 1 (diluting 1000 times) | + (a little morbidity) |
| Non-processor | +++(morbidity, petal decolouring) |
Interpretation of result by table 5 can confirm that microbial pesticide composition of the present invention has sufficient control efficiency for plant disease.
＜embodiment 5 〉
In microbial pesticide composition of the present invention, studied and in fact whether to have reduced the crop surface medicine spot that produces owing to the spreading microbial pesticide composition.
Prepare the 1000 times of dilutions and the eggplant (kind: 1,002 No. 2) of 1000 times of dilutions, the comparative example 6 of formulation example 2, carry out the processing identical, study the outward appearance of eggplant then with embodiment 4 (1).The result is shown in table 6.
Table 6
| Medicament | Extension rate | Leaf | Fruit |
| Comparative example 6 | 1000 | ++ | ++ |
| Formulation example 2 | 1000 | - | + |
-: can not confirm the pollution that causes by the medicine spot.
+: can confirm the light contamination that causes by the medicine spot.
++: can confirm the obvious pollution that causes by the medicine spot.
By interpretation of result shown in the table 6 as can be known, if use microbial pesticide composition of the present invention, in fact reduced the crop surface medicine spot that produces by the spreading microbial pesticide composition.
Practicality on the industry
Can provide by the present invention and to reduce the crop surface medicine spot that is produced by the spreading microbial pesticide composition, and the microbial pesticide composition with abundant controlling plant diseases effect.
Claims (13)
1. microbial pesticide composition, it contains microorganism with controlling plant diseases effect, contains oxygen mineral salt and urea.
2. according to the microbial pesticide composition described in the claim 1, wherein, the aforementioned oxygen mineral salt that contain are 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, and aforementioned urea is 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
3. according to the microbial pesticide composition described in claim 1 or 2, wherein, aforementioned micro organism is 10～30 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
4. microbial pesticide composition, it contains microorganism with controlling plant diseases effect, contains oxygen mineral salt, urea and surfactant.
5. according to the microbial pesticide composition described in the claim 4, wherein, the aforementioned oxygen mineral salt that contain are 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, aforementioned urea is 10～50 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount, and aforementioned surfactants is 5～30 weight % with respect to the content of aforementioned micro organism composition pesticide total amount.
6. according to the microbial pesticide composition described in claim 4 or 5, wherein, aforementioned micro organism is 10～30 weight % with respect to aforementioned micro organism, the aforementioned content that contains oxygen mineral salt and aforementioned urea total amount.
7. according to the microbial pesticide composition described in any one of the claim 4～6, wherein, aforementioned surfactants is an anionic surfactant.
8. according to the microbial pesticide composition described in any one of the claim 1～7, wherein, the aforementioned oxygen mineral salt that contain are carbonate or sulphate.
9. the microbial pesticide composition described in according to Claim 8, wherein, aforementioned carbonate is sodium bicarbonate.
10. according to Claim 8 or the microbial pesticide composition described in 9, wherein, aforementioned sulphate is sodium sulphate.
11. according to the microbial pesticide composition described in any one of the claim 1～10, wherein, aforementioned micro organism is a bacillus.
12. according to the microbial pesticide composition described in the claim 11, wherein, aforementioned bacillus is a bacillus subtilis.
13. according to the microbial pesticide composition described in the claim 11, wherein, aforementioned bacillus is NCIB12376 strain or NCIB12616 strain.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003311582A JP2005075813A (en) | 2003-09-03 | 2003-09-03 | Microbial pesticide composition |
| JP311582/2003 | 2003-09-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1845677A true CN1845677A (en) | 2006-10-11 |
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ID=34269704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800253777A Pending CN1845677A (en) | 2003-09-03 | 2004-08-11 | Microbial pesticide composition |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2005075813A (en) |
| KR (1) | KR20060125696A (en) |
| CN (1) | CN1845677A (en) |
| TW (1) | TW200511932A (en) |
| WO (1) | WO2005023004A1 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3554592B2 (en) * | 1994-12-20 | 2004-08-18 | 出光興産株式会社 | Bacillus spore fraction and plant disease control method using the spore fraction |
| JPH08175921A (en) * | 1994-12-22 | 1996-07-09 | Idemitsu Kosan Co Ltd | Agricultural and horticultural germicidal composition |
-
2003
- 2003-09-03 JP JP2003311582A patent/JP2005075813A/en active Pending
-
2004
- 2004-08-11 CN CNA2004800253777A patent/CN1845677A/en active Pending
- 2004-08-11 WO PCT/JP2004/011532 patent/WO2005023004A1/en active Application Filing
- 2004-08-11 KR KR1020067004365A patent/KR20060125696A/en not_active Application Discontinuation
- 2004-09-03 TW TW093126725A patent/TW200511932A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| TW200511932A (en) | 2005-04-01 |
| WO2005023004A1 (en) | 2005-03-17 |
| KR20060125696A (en) | 2006-12-06 |
| JP2005075813A (en) | 2005-03-24 |
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