JP2003206212A - Plant disease injury-controlling method and composition therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a plant disease injury-controlling method having high controlling effect on various plant disease injuries with expanded range of controllable disease injuries, and to provide a composition for use in the method. <P>SOLUTION: This plant disease injury-controlling method comprises simultaneously using Trichoderma atroviride SKT-1 strain (FERM P-16510) and/or Trichoderma atroviride SKT-3 strain and another kind of microorganisms having plant disease injury-controlling effect and/or separately using these two kinds of microorganisms in different period of times during the identical cultivation period. The composition for use in this method is also provided. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a microorganism Trichoderma atrobilide SKT-1 strain (FERM P-16510) and / or a fungicide benomyl highly resistant mutant strain derived from the SKT-1 strain, which is effective for controlling plant diseases. Trichoderma atrobilide SKT-3 strain (FERM P-16511) and the above-mentioned Trichoderma atroviride SKT-1 strain and Trichoderma trichoderma
A method for controlling plant diseases by mixing and / or using microorganisms such as filamentous fungi other than Atroviride SKT-3 strain, bacteria, actinomycetes at different treatment times during the same cultivation period (hereinafter referred to as system use) The present invention relates to a plant disease control composition and a method for producing the same.

[0002]

2. Description of the Related Art Plant disease control methods include cropping and physical control such as rotation and utilization of solar heat, chemical control with chemical pesticides, use of disease resistant varieties, and natural enemies, attenuated viruses, antagonistic microorganisms, etc. The biological control using is mentioned. Among them, chemical pesticides, especially the research and development of organic synthetic bactericides, have been developed remarkably, have higher potency, have been developed one after another with a large number of various actions, and further various application methods have been developed. The chemical control methods using these have greatly contributed to disease control and labor saving of the control work.
However, in recent years, a phenomenon in which the control effect is reduced due to the emergence of so-called drug-resistant bacteria has been observed in some crops and diseases, and has become a problem. Further, as crops have been designated as producing areas, continuous cropping is inevitable, and as a result, the occurrence of soil-borne diseases, which are difficult to control with chemical pesticides, has become a serious problem in various regions.

Against this background, in recent years, a control system biased toward the use of chemical pesticides has been reviewed, and microbial pesticides using microorganisms that are supposed to have higher environmental safety than chemical pesticides have been proposed. Some are reaching the stage of practical application.

In general, microbial pesticides have a narrower range of diseases that can be effectively controlled as compared with chemical pesticides, and the control effect is often unstable. In addition, conventional microbial pesticides exert a disease control effect by treating the plant or soil alone with the microorganisms, but an attempt to utilize two or more kinds of microorganisms for disease control, or No attempt has been made to systematically use two or more kinds of microorganisms and utilize them for disease control.

[0005]

The problem to be solved by the present invention is to enhance the effect of controlling various plant diseases using microorganisms which are supposed to have high environmental safety, and to control the range of diseases. Is to expand.

[0006]

The present invention provides a microorganism Trichoderma atrobilide effective for controlling plant diseases.
rma atroviride) SKT-1 strain (FERM P-16510) and /
Or Trichoderm atrobilide, a fungicide highly resistant mutant of benomyl derived from SKT-1 strain
a atroviride) SKT-3 strain (FERM P-16511) and a mixture of microorganisms having a plant disease controlling effect other than the above-mentioned Trichoderma atroviride SKT-1 strain and Trichoderma atroviride SKT-3 strain, or a plant disease controlling method using a system, and The present invention provides a plant disease control composition for mixed use and a method for producing the same. Also,
Trichoderma atrobilide SKT-1 strain and / or Trichoderma atroviride SKT-3 strain and the above-mentioned Trichoderma atroviride SKT-1 and Trichoderma atrobilide SKT-3 microorganisms having no plant disease control effect, and the Trichoderma atroviride SKT-1 strain and / or trichoderma By coexistence with the Atrobilide SKT-3 strain, the above-mentioned Trichoderma atrobilide SKT
-1 strain and / or Trichoderma atrobilide SKT-
It is intended to provide a plant disease control method characterized by being used in combination with a microorganism having an action of enhancing the plant disease control effect of 3 strains, a plant disease control composition used in the mixed use, and a method for producing the same.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be specifically described below. Examples of microorganisms used for mixing and / or systematic use in the present invention will be given, but the present invention is not limited to these examples.

Pseudoperonospor
a) spp., Venturia spp., Erysiphe spp., Pyricularia spp., Botrytis spp., Rhizoctonia spp.
octonia spp., Puccinia spp., Septoria spp., Sclerotini
a) Genus, Pythium, Trichoderma (Tr)
ichoderma spp., Fusarium spp., Rhizopus spp., Mucor spp., Corticium spp., Phoma spp., Phytophthora spp., Coriobolus spp.
chliobolus), Claviceps (Claviceps),
Ceratobasidium spp., Aspergillus spp., Filamentous fungi, Burkholderia, Clostridiu
m) spp., Bacillus spp., Pseudomonas spp., Acidovorax
Genus bacterium, Enterobactor genus, Serratia genus, Erwinia genus, Acetobacter, Agrobacterium (A
grobacterium), Xanthomonas spp.,
Bacteria such as Gluconobacter spp., Rhizobacter spp., Claviobacter (C
genus lavibacter, Arthrobacter
Genus spp, Curtobacterium spp, Rhodococcus spp, Streptomyces spp, Micrococcu
s) Actinomycetes, Saccharopolyspora (genus Saccharopolyspora), Corynebacterium (genus Corynebacterium), actinomycetes such as Micromonospora (genus Micromonospora) can be used.

Microorganisms having an effect of controlling plant diseases other than the Trichoderma atroviride SKT-1 strain, SKT-3 strain and Trichoderma atrobilide SKT-1 strain and SKT-3 strain, Trichoderma atroviride SKT-1 strain and SKT strain. -3 strains having no plant disease control effect and coexisting with the Trichoderma atrobilide SKT-1 strain and / or the SKT-3 strain, whereby the Trichoderma atroviride SKT-1 strain and the SKT-3 strain When a microorganism having an action of enhancing a plant disease controlling effect is used as a disease controlling agent, spores or cultured cells of each microorganism may be mixed and / or used continuously as a system. In addition, each microorganism is a carrier,
A mixture of surfactants, dispersants, auxiliary agents, etc., and formulated into a form such as powder, granules, wettable powder, wettable granules, and flowable agent by a conventional method and / or systemically used. You may. Further, in the case of mixed use, spores or cultured bacterial cells of these two or more kinds of microorganisms may be mixed in advance and a formulation prepared by the above method may be used. The spore suspension can be used in the same manner as the above-mentioned flowable agent without special formulation.

As described above, in formulating these microorganisms, a water-soluble carrier or a water-insoluble carrier can be used as a suitable carrier, and these carriers can be used in combination. The water-soluble carrier includes, for example, organic or inorganic acid salts such as ammonium sulfate, ammonium bicarbonate, ammonium nitrate, ammonium chloride, potassium chloride, sodium sulfate, magnesium sulfate, sodium citrate, sodium carbonate, sodium hydrogen carbonate, citric acid and succinic acid. Organic acid salts such as, sugars such as sucrose and lactose,
Urea etc. can be mentioned. As the water-insoluble carrier, fine powder of mineral substances is generally used, and examples thereof include clays, calcium carbonate, talc, diatomaceous earth, and bentonite. Examples of the water-insoluble non-mineral fine powder extender include white carbon. The blending ratio of these fillers can be blended as needed, but can be 1 part by mass to 99 parts by mass, preferably 10 parts by mass to 90 parts by mass with respect to 100 parts by mass of the composition. Examples of surfactants and dispersants include nonionic surfactants such as polyethylene glycol higher fatty acid ester, polyoxyethylene alkyl ether, polyoxyethylene alkyl aryl ether, polyoxyethylene allyl phenyl ether, sorbitan monoalkylate, and alkyl aryl sulfone. Acid salt, alkyl sulfosuccinate, lignin sulfonate, naphthalene sulfonate and its condensate, alkyl sulfate ester salt, alkyl phosphate ester salt, alkyl aryl sulfate ester salt, alkyl aryl phosphate ester salt, polyoxyethylene alkyl ether sulfate Ester salts, polyoxyethylene alkylaryl ether sulfate ester salts, polyoxyethylene allyl phenyl ether phosphates, polycarboxylic acid type polymer activators, etc. Anion surfactants or the like, can be cited silicone, fluorinated, the soaps surfactant. Anionic surfactants are particularly preferable. The mixing ratio of these surfactants is usually 0.1 part by mass to 20 parts by mass, preferably 0.1 part by mass with respect to 100 parts by mass of the composition.
The amount can be 5 parts by mass to 10 parts by mass, more preferably 2 parts by mass to 7 parts by mass. Silicone-based, fluorine-based, and soaps can also be used as a defoaming agent. As an auxiliary agent,
Examples thereof include carboxymethyl cellulose, dextrin, polyoxyethylene glycol, gum arabic, xanthan gum, guaceed gum, starch, lactose, sucrose, polyvinylpyrrolidone, polyvinyl alcohol and the like. The blending ratio of these auxiliary agents is usually 0.01 parts by mass to 10 parts by mass, preferably 0.1 parts by mass to 5 parts by mass with respect to 100 parts by mass of the composition.

Next, the control method of the present invention will be exemplified. First, when it is used for controlling a disease occurring on the above-ground part, it is appropriately diluted with water or the like, and then sprayed on the whole plant with a sprayer to exert a preventive or therapeutic effect. Further, when used for the control of seed-borne disease or soil-borne disease, in the case of spores, cultured bacterial cells or viable bacterial preparations, soaking seeds or roots, spraying, coating or dressing treatment, or directly to the soil By mixing or suspending in water or the like and then irrigating, it acts on pathogenic fungi and plants in seeds or soil, and exerts a controlling effect. As the usage amount,
The type of microorganism to be used, the dosage form of the preparation, the method of application, the place of application, the type of disease to be applied, the desired control effect, etc. are selected appropriately, depending on the powder, granules, or formulations diluted with water In this case, the spore concentration of the fungus is 10 per ml.
^It is desirable to use in the range of about ² to 10 ¹¹ , preferably 10 ⁴ to 10 ¹⁰ .

[0012]

EXAMPLES The present invention will now be specifically described with reference to examples, but the present invention is not limited to these examples.

(Example 1: Wettable powder) 8 parts by weight of conidia formed by culturing Trichoderma atrobilide SKT-1 strain or Trichoderma atrobilide SKT-3 strain on a PDA plate medium for 7 to 14 days , Serratia marcescens IFO-4002 strain P
4 parts by mass, 40 parts by mass of diatomaceous earth, 46 parts by mass of clay, 1 part by mass of sodium alkylnaphthalene sulfonate, and 4 parts by mass of cells obtained by repeating centrifugation and resuspension several times from a culture solution cultured for 1 day in a liquid medium D. 1 part by mass of sodium lignin sulfonate was mixed and dried, and then pulverized to obtain a wettable powder.

(Example 2: Rice scab seedling disease control effect test)
Trichoderma atrobilide SKT-1 strain or SKT-
The 3 strains were cultured on a PDA medium for 10 days, and the conidia obtained were suspended in distilled water to prepare a spore suspension. Also, Fusarium oxysporum SN
The F-356 strain (Japanese Patent Laid-Open No. 11-89562), which had been shake-cultured in a PD medium for 3 days, was appropriately diluted to prepare a spore suspension. In addition, Streptovirtisillium (Strepto
verticillium) FERM P-120105 strain was shake-cultured in PD medium for 1 day, and appropriately diluted to prepare a similar suspension. After immersing rice sprouting seedling diseased rice (cultivar is Tanginbozu, spray inoculation of rice sapling seedling fungus at the flowering stage of rice) in a mixture of these bacterial suspensions alone or at a water temperature of 15 ° C. for 5 days, Discard the bacterial solution, 32 ℃, 100% humidity
And kept it for 1 day to promote germination. The seeds are sown in a plastic cup having a diameter of 6 cm filled with seedling cultivation soil, kept in a seedling storage room at 30 ° C for 3 days after seeding, and further kept at 15-25 ° C.
After controlling for about 20 days in the glass greenhouse, the presence or absence of disease was investigated for all seedlings, and the diseased seedling rate was calculated by Formula 1, and the control value was calculated by Formula 2. The seeding amount per one section was equivalent to 3 g of dry paddy (about 90 to 100 grains), and the test was repeated 3 times. The results are shown in Table 1.

[0015]

[Formula 1]

[0016]

[Formula 2]

[0017]

[Table 1]

The results are shown in Table 1, SKT-1 strain, S
By mixing KT-3 strain, SNF-356 strain, and P-120105 strain, compared to the use of each strain alone,
Clearly, the effect of controlling rice scab seedling disease was enhanced.

(Example 3: Rice seedling bacterial bacterial disease control effect test) Trichoderma atrobilide SKT-1 strain or SK
The T-3 strain was cultured on a PDA medium for 10 days, and the conidia obtained were suspended in distilled water to prepare a bacterial suspension. Also,
Fusarium oxysporum S
The NF-356 strain was shake-cultured in a PD medium for 3 days and appropriately diluted to prepare a bacterial suspension. A suspension of these bacterial suspensions alone or in a mixture was used to soak rice seedling bacterial wilt disease cultivated in rice (variety is golden fine, spray inoculation of rice seedling bacterial germ during flowering of rice) at a water temperature of 15 ° C for 5 days. After soaking (immersion treatment), the bacterial solution was discarded, and germination was promoted by keeping it at 32 ° C. and 100% humidity for 1 day. The seeds were sown in a plastic cup having a diameter of 6 cm filled with seedling cultivation soil. Also, in the irrigation process, 10 ml of these bacterial liquids per cup at the time of seeding
Irrigated each. After seeding, the seedlings are kept for 3 days at 30 ° C., and the seedlings are kept in a humid chamber at 25-30 ° C. for about 10 days. After that, all seedlings are examined for the degree of disease according to the criteria shown in Table 2, and diseased according to Formula 3. And the control value was calculated by Formula 4. The seeding amount per plot is equivalent to 3 g of dry paddy (about 90
~ 100 grains), and the test was repeated 3 times. The results are shown in Table 3.

[0020]

[Table 2]

[0021]

[Formula 3]

[0022]

[Formula 4]

[0023]

[Table 3]

The results are shown in Table 3, SKT-1 strain, S
By mixing the KT-3 strain and the SNF-356 strain or using them systematically, the effect of controlling the bacterial seedling blight of rice was clearly enhanced as compared with the use of each strain alone.

(Example 4: Cucumber gray mold control effect test) Trichoderma atrobilide SKT-1 strain or Trichoderma atrobilide SKT-3 strain was cultured on a PDA plate medium for 14 days, and the conidia obtained were distilled. It was suspended in water to prepare a spore suspension. See also Micro Monospora
Carbonasia (Micromonospora carbonacea) IFO-
14108 strain, Bacillus cereu
s) The IFO-3001 strain was shake-cultured in a PD medium for 1 day and appropriately diluted to prepare a similar suspension. 9c
30 ml per pot of the cucumber seedlings at the cotyledon stage (cultivar: Sagamihanjiro, 10 stems planted) cultivated in a square plastic square bowl
It was sprayed using a spray gun. After air-drying, a mycelial suspension of cucumber gray mold (Botrytis cinerea) was spray-inoculated and immediately placed in a humid chamber at 20 ° C. Two days after inoculation, the degree of disease was investigated for each cotyledon according to the criteria in Table 4, and the formula 5
The degree of disease was calculated by the formula, and the control value was calculated by the formula 4 from the ratio with the untreated plot. The test was performed in triplicate and the results are shown in Table 5.
It was shown to.

[0026]

[Table 4]

[0027]

[Formula 5]

[0028]

[Table 5]

The results are shown in Table 5 by comparing the SKT-1 strain or the SKT-3 strain with the IFO-14108 strain and / or the IFO-3001 strain, as compared with the use of each strain alone. The effect of controlling gray mold of cucumber was increased.

Example 5 Bentgrass Red Burn Disease Control Effect Test A 6 cm diameter plastic cup was filled with sterilized sand, and bentgrass seeds were sown at a rate of 8 g per m 2, 8
The bentgrass cultivated in a greenhouse kept at a temperature of 15 to 25 ° C. for 10 weeks was subjected to a control effect test. As the management during the cultivation period, liquid fertilizer was appropriately irrigated every 7 to 10 days after 10 days after seeding, and pruning was performed every 3 to 5 days from the same period. Bentgrass cultivated in this pot was added to the SKT-1 strain or SKT-3 strain prepared in Example 1 and I.
A wettable powder containing the FO-4002 strain or a diluted wettable powder made by removing one of the strains was used in an amount of 10 per 1 pot.
The cells were irrigated with a spray gun. In addition, Pythiu separated from the diseased stems of bentgrass after about 6 hours of treatment
Bentgrass seed medium (100 ml
Bentgrass seeds (2 g) and distilled water (5 ml) were placed in a Kolben, and the cultivated cells were cultivated in a sterilized medium at 27 ° C. for 7 days, crushed in a mortar, and then diluted with sterile sand at a volume ratio of 1:50. Inoculation was carried out by rubbing into the bentgrass edge part at a rate of 10 g per 100 cm ^{2 using the above} as the inoculum source. After the inoculation, it was controlled in a moist chamber maintained at 30 ° C to promote the onset of disease. Four days after the inoculation, the degree of disease was investigated for each pot according to the criteria shown in Table 6, the disease degree was determined by the equation 6, and the control value was calculated by the equation 4 from the ratio with the untreated plot. The test was performed in 5 replicates and the results are shown in Table 7.

[0031]

[Table 6]

[0032]

[Formula 6]

[0033]

[Table 7]

The results are shown in Table 7, by treating the mixed wettable powder of the SKT-1 or SKT-3 strain with the IFO-4002 strain, as compared with the single wettable powder of each strain, The effect of controlling red grass disease of bentgrass was obviously enhanced.

Example 6 Wheat wilt disease control effect test
Trichoderma atrobilide SKT-1 strain or SKT-
The 3 strains were cultured on a PDA medium for 10 days, and the conidia obtained were suspended in distilled water to prepare a spore suspension. Also Bacillus cereus IFO-3001
The strain was shake-cultured in PD medium for 1 day and appropriately diluted to prepare the same suspension. 10 seeds of wheat seeds (variety: Norin 61) were sown in each of the plastic pots with a diameter of 6.0 cm, and grown in a greenhouse to develop the second leaves.
10 ml per one pot was sprayed with the above-mentioned bacterial suspension alone or as a mixture. After air-drying, wheat bacterial wilt (Sept
Oria nodorum) spores were inoculated and kept in a greenhouse.
Ten days after the inoculation, the diseased area of the first leaf was investigated, the disease degree was calculated by the formula 5, and the control value was calculated by the formula 4 from the ratio with the untreated plot. The test was performed in triplicate, and the results are shown in Table 9.

[0036]

[Table 8]

[0037]

[Table 9]

The results are shown in Table 9, SKT-1 strain, S
These strains were also used against wheat wilt disease in which a high control value was not obtained even when the KT-3 strain was treated at a relatively high concentration.
By using the IFO-3001 strain in combination, the effect of controlling wheat wilt disease was obviously enhanced as compared with the use of each strain alone.

[0039]

The Trichoderma atrobilide SKT-1 strain, the SKT-3 strain according to the present invention and a microorganism having a plant disease controlling effect other than the Trichoderma atroviride SKT-1 strain and the Trichoderma atrobilide SKT-3 strain are mixed and / or continuous. The method for controlling plant diseases, which is characterized by treating, is highly effective against various crop diseases by foliage treatment on plants, dipping or dressing treatment on plant seeds or plant roots, and further irrigating or admixing soil. A control effect can be expected. In addition, the range of diseases that exert a controlling effect can be expanded, which is useful for agricultural production.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A01N 25/14 A01N 25/14 25/30 25/30 63/04 63/04 A // C12N 1/14 C12N 1/14 AF term (reference) 4B065 AA70X AC20 CA47 4H011 AA01 BA01 BA05 BA06 BB21 BC07 BC18 BC19 BC20 DA15 DC01 DC08 DD03 DD04 DE15 DH10

Claims (16)

[Claims] 1. Trichoderma Atrobilide
rma atroviride) SKT-1 strain (FERM P-16510) and /
Or Trichoderma atroviide
ride) SKT-3 strain (FERM P-17021) and a mixture of Trichoderma atroviride SKT-1 strain and a microorganism having a plant disease controlling effect other than Trichoderma atroviride SKT-3 strain, and / or different treatment during the same cultivation period A method for controlling plant diseases, which is characterized in that it is used at different times. 2. Trichoderma Atrobilide
rma atroviride) SKT-1 strain (FERM P-16510) and /
Or Trichoderma atroviide
ride) SKT-3 strain (FERM P-17021) and the above-mentioned Trichoderma atroviride SKT-1 strains, which are microorganisms having no plant disease controlling effect other than the above-mentioned Trichoderma atroviride SKT-1 strain and Trichoderma atroviride SKT-3 strain.
Strain and / or Trichoderma atroviride SKT-3 strain, whereby the above-mentioned Trichoderma atroviride SK
T-1 strain and / or Trichoderma atrobilide SKT
-3. A method for controlling plant diseases, which is characterized in that it is used in combination with a microorganism having an action of enhancing the plant disease controlling effect of the strain-3. 3. The microorganism having a plant disease controlling effect according to claim 1 or the microorganism having an action of enhancing the plant disease controlling effect according to claim 2, is selected from at least one kind of filamentous fungi, bacteria and actinomycetes. A method for controlling plant diseases, which comprises: 4. The filamentous fungus according to claim 3, wherein the genus Pseudoperonospora, the genus Venturia, the genus Erysiphe, the genus Pyricularia, the Botrytis.
s) genus, Rhizoctonia genus, Puccinia genus, Septoria genus, Sclerotinia genus, Pythium
um genus, Trichoderma genus, Fusarium genus, Rhizopus genus, Mucor genus, Corticium genus, Phoma genus, Phytophth
ora), Cochliobolus, Claviceps, Ceratbacillium
obasidium), Aspergillus (Aspergillus) (Aspergillus) at least 1 sort (s) or more selected from the plant disease control method characterized by the above-mentioned. 5. The bacteria according to claim 3, wherein the bacteria are Burkholderia and Clostridiu.
m) spp., Bacillus spp., Pseudomonas spp., Acidovorax
Genus bacterium, Enterobactor genus, Serratia genus, Erwinia genus, Acetobacter, Agrobacterium (A
grobacterium), Xanthomonas spp.,
A method for controlling plant diseases, which is selected from at least one species of Gluconobacter spp. And Rhizobacter spp. 6. The actinomycete according to claim 3, wherein the actinomycete is a genus Clavibacter, Arthrob.
acter), Curtobacterium
Genus, Rhodococcus, Streptomyces, Micrococcus
crococcus), Saccharopolys
pora), Corynebacterium
A method for controlling plant diseases, which is selected from at least one or more species belonging to the genus Microbacterium and genus Micromonospora. 7. Trichoderma Atrobilide
rma atroviride) SKT-1 strain (FERM P-16510) and /
Or Trichoderma atroviide
ride) SKT-3 strain (FERM P-17021) and a composition for controlling plant diseases, which comprises a microorganism having a plant disease controlling effect other than the above-mentioned Trichoderma atrobilide SKT-1 strain and Trichoderma atrobilide SKT-3 strain . 8. Trichoderma Atrobilide
rma atroviride) SKT-1 strain (FERM P-16510) and /
Or Trichoderma atroviide
ride) SKT-3 strain (FERM P-17021) and the above-mentioned Trichoderma atroviride SKT-1 strains, which are microorganisms having no plant disease controlling effect other than the above-mentioned Trichoderma atroviride SKT-1 strain and Trichoderma atroviride SKT-3 strain.
Strain and / or Trichoderma atroviride SKT-3 strain, whereby the above-mentioned Trichoderma atroviride SK
T-1 strain and / or Trichoderma atrobilide SKT
-3. A composition for controlling plant diseases, which comprises a microorganism having an action of enhancing the plant disease controlling effect of the 3 strain. 9. The composition for controlling plant diseases according to claim 7, which further contains a surfactant. 10. The composition for controlling plant diseases according to claim 9, wherein the surfactant is an anionic surfactant. 11. The composition for controlling plant diseases according to claim 7, which is a solid pesticide formulation. 12. The solid agricultural chemical formulation is a powder, wettable powder, granule,
The composition for controlling plant diseases according to claim 11, which is a wettable granule. 13. The composition for controlling plant diseases according to claim 7, which is a liquid pesticide formulation. 14. The composition for controlling plant diseases according to claim 7, which is a spore suspension. 15. Trichoderma atrobilide (Tricho
derma atroviride) SKT-1 strain (FERM P-16510) and
/ Or Trichoderma atrovide
iride) SKT-3 strain (FERM P-17021) and a microorganism having a plant disease controlling effect other than the Trichoderma atroviride SKT-1 strain and the Trichoderma atroviride SKT-3 strain, and a plant disease controlling composition characterized by the above-mentioned. Manufacturing method. 16. Trichoderma atrobilide (Tricho
derma atroviride) SKT-1 strain (FERM P-16510) and
/ Or Trichoderma atrovide
iride) SKT-3 strain (FERM P-17021) and microorganisms having no plant disease control effect other than Trichoderma atroviride SKT-1 strain and Trichoderma atroviride SKT-3 strain, and Trichoderma atroviride SKT-
1 strain and / or Trichoderma atrobilide SKT-3
Due to coexistence with the strain, Trichoderma astrobilide S
KT-1 strain and / or Trichoderma atrobilide SK
A method for producing a plant disease controlling composition comprising a microorganism having an action of enhancing the plant disease controlling effect of the T-3 strain.