KR20050034271A - Antimicrobial composition containing natural extract, nano silver and natural essential oil - Google Patents

Abstract

The present invention is to provide a composition having excellent antibacterial activity consisting of natural extract grapefruit, machi, Echochocho extract and nano silver and natural essencell oil.

The antimicrobial composition of the present invention is 10 to 30% by weight of the natural extract consisting of a mixture of grapefruit, machi, and fish herb in a weight ratio of 1: 1: 1, nanosilver 1-5% by weight of 50-1000 ppm, one or more natural essentials. The oil mixture is characterized in that 5 to 30% by weight and the remainder consists of ethanol.

The antimicrobial composition according to the present invention exhibits excellent antimicrobial activity against bacteria and fungi such as Staphylococcus aureus, skin flora, and skin filamentous fungi, and is suitable for preparing formulations of hand cleaners, aromatic fragrances, and herbal gels containing the same. Do.

Description

Antimicrobial composition containing natural extract, nano silver and natural essential oil

The present invention relates to a composition excellent in antimicrobial activity consisting of grapefruit, machi, eochocho extract and nano silver and natural essencell oil.

Various microorganisms exist in the skin, and these microorganisms cause various skin diseases. For example, Staphylococcus aureus , which causes purulent in the skin, Staphylococcus epidermis , Propionibacterium acnes , Pity ovale ( Pity ovale ), which breaks down sebum or sweat secreted on the skin and produces an odor. In particular, Propionibacterium acnes decomposes sebum using fatty acid degrading enzyme lipase to generate free fatty acids. These free fatty acids not only irritate the skin but also cause inflammatory lesions such as papules, pustules and nodules, which are red rashes found in acne.

Skin fungi (dermatophytes) produces a superficial fungus invades the stratum corneum of the skin and hair. The skin filaments that cause superficial mycosis include Microsporum , Trichophyton and Epodermophyton , which represent ringworm. Candida albicans also causes cutaneous candidiasis and chronic mucosal candidiasis. These fungi represent superficial mycosis, in which the fungus parasitic in the keratinous tissue of the skin to reproduce and cause lesions. Another cause of granulomatous lesions in skin and subcutaneous tissue is Sporotriz schenickii .

Many herbal medicines have been known for a long time in skin diseases caused by bacteria and fungi. The research to scientifically prove the effectiveness of conventional medicinal herbs used and handed down conventionally, and to find a herbal ingredient with a new antibacterial activity has been actively conducted in recent years.

The most widely known antibacterial medicines are berberine, an alkaloid contained in Phellodendron amurense , Coptus japonica , etc. It is listed as berberine hydrochloride and has a broad antibacterial spectrum. It is widely used in diseases, and strong antimicrobial activity against acne causing bacterium propionibacterium has been reported.

Accordingly, an object of the present invention is to provide an antimicrobial composition containing natural extracts, nanosilver and natural essential oils that exhibit excellent antimicrobial activity against bacteria and fungi such as Staphylococcus aureus, skin flora, and skin filamentous fungi.

It is an object of the present invention to provide an antimicrobial composition containing natural extracts, nanosilver and natural essential oils suitable for use in the formulation of hand cleaners, aroma fragrances and herbal gels.

The antimicrobial composition of the present invention for achieving the above object is 10 to 30% by weight of the natural extract consisting of a mixture of grapefruit, machihyeon and eochochoche in a weight ratio of 1: 1 to 1, 1 to 5 weight of nanosilver with a concentration of 50 to 1000ppm %, 5-30% by weight of one or more natural essential oil mixtures and the remainder consisting of ethanol.

Hereinafter, the present invention will be described in detail.

In the present invention, as a natural extract using grapefruit, machihyeon, eoseongcho, grapefruit belongs to citrus (Citrus) and the size is slightly larger than the adult fist, the shell is smooth and pale yellow. The flesh is very soft, succulent and white. The fruit is sweet and sour with a bitter bitter taste. Grapefruit seed extract (GSE) blocks the supply of amino acids in the cellular tissue and has a low molecular weight. It is a component that is attracting attention as one of the most versatile and non-toxic bactericidal agents from the medical field by killing microorganisms through the decomposition and elution of intracellular substances.

According to Korean Agricultural Products Storage Distribution Association 1, 1 ~ 7 (1994), grapefruit seed extract (GSE) has antimicrobial activity against a wide range of decaying microorganisms, and the minimum concentration of grapefruit seed extract (GSE) against various decaying microorganisms is In case of 10 ~ 500ppm, mold 250 ~ 1,000ppm, yeast 100 ~ 250ppm is mentioned.

According to the Korean Fisheries Society 23 (4), 297 ~ 302 (1990), the vibrio genus was 50ppm, Bacillus cereus and Staphylococcus aureus in the antibacterial activity of grapefruit seed extract (GSE) against bacteria. ) Is inhibited in the growth of bacteria at 30ppm or more, and has a bactericidal effect at 50ppm or more for Vibrio vulnificus .

In addition, according to Korean Journal of Nutrition and Nutrition 24 (5), 690-694 (1995), acute oral toxicity, skin irritation and acute effects were investigated to examine the stability of grapefruit seed extract (GSE), which is considered to be widely used as a natural antibacterial agent. As a result of eye irritation test, the acute LD50 value of experimental rats is 3.75g / kg, and the toxicity to skin is weak, and it is said that there are few lesions such as edema but eye irritation.

According to the Korean Journal of Food Hygiene and Safety 10 (4), 263 ~ 270 (1995), grapefruit seed extract (GSE) is an Entrerobacter Pyrinus, a fruit vegetable perishable microorganism. It has been reported that the antimicrobial effect of grapefruit seed extract (GSE) can be clearly observed by inhibiting the growth of bacteria at low treatment concentrations below 500 ppm against black fungal disease (Fusarium sp.) .

On the other hand, one of the natural extracts constituting the antimicrobial composition according to the present invention, Eungseongcho is a perennial herb belonging to over three hundreds, which breeds better in wetlands and has a bad smell like fishy fish in the whole plant, and the stem of the ground is white and soft. It extends straight to the side, and the main stem on the ground is hairless with leaves, grows straight to 20-50cm in height, and has several longitudinal lines. Because of the strong fishy smell in the grass (漁: meat fish), eoseongchae or chaechae called 10 kinds of military service to heal it is called a medicine (十 藥). Roots, leaves, stems, grasses, herbs, syphilis, cystitis, uterus, edema, phenone, otitis media, moorings, para stroke, pneumonia, dermatitis, hepatitis, hypertension, cardiac fever, diuresis, gonorrhea, remission, urethritis, etc. I use it as a medicine.

Eoseongcho is the most famous folk medicine, and the juice of fresh leaves is effective when applied to purulent, boils, wounds, etc. It also lowers venom and diuretic effect. For gonorrhea and urethritis, 30g of Eoseongcho is drunk and also used to wash hemorrhoids and scabies with decoction of this month, and it is also used as a bath (medicinal plant dictionary). In the private sector, juices can be attached to athlete's foot, hemorrhoids, snake venom, and lacquer, and are good for fever, detoxification, diuresis and eczema, and for wounds, washing face, hookworm, skin disease, and gastrointestinal diseases.

Echochocho like this is effective in the regeneration of skin tissue and wound treatment, antibacterial, antifungal effect, and also effective in preventing skin aging by promoting the antioxidant effect and blood circulation.

Machihyeon, on the other hand, has no toxicity and has excellent efficacy in treating skin diseases such as boils and edema without irritation, and has been widely used as a medicine. In the treatment of copper, detoxification, drainage, diuretic effect of dysentery, enteritis, bacterial strains were eaten, used for swelling, scabies, eczema, etc. In addition to the insect poisons used to solve the snake venom and also acts to control a number of different diseases. Herbs are also eaten, so there is no side effect even if you use a large amount. This effect is effective in dealing with side effects that may be caused by skin diseases or cosmetic poisoning caused by cosmetics or other environment. In addition, Machi Prefecture contains natural moisturizing factors such as saccharides (Polysaccharides) and amino acids (Amino acids), which contains a lot of water in the event of a severe drought and is very difficult to dry. In other words, Machi has anti-inflammatory and skin irritant effects. In the antimicrobial composition of the present invention, the grapefruit, machi, and fish vinegar are used as a natural extract by mixing in a weight ratio of 1: 1: 1, and the amount of the natural extract is 10 to 30% by weight, preferably 1 to 5% by weight. It is good.

Here, if the natural extract is used in less than 10% by weight, the antimicrobial effect of the antimicrobial effect is less effective than the expected antibacterial effect, on the contrary, when used in excess of 30% by weight of nano silver and essential oil Since there is a problem that synergy is suppressed, it is preferable to use within the above range.

On the other hand, if the silver (silver) into a nano state has a strong sterilization and antibacterial properties. According to the research report, it is able to sterilize 650 kinds of bacteria and viruses, and it is known to have an excellent effect on fungi. In addition, nano silver has several times stronger sterilizing power than chlorine series, but is harmless to the human body. In general, pure nano silver is recognized as having no abnormality in human body even if it is eaten. The antimicrobial action of such nanosilver is estimated to work by three mechanisms.

First, silver cations (Ag +) bind strongly to bacteria -SH, -COOH, and -OH groups to destroy bacterial cell membranes or disrupt cell functions. Second, nano-colloidal silver catalyzes Oxygen is converted into active oxygen (O ²⁺ , O ^2- , O) to sterilize and sterilize. As a result, oxygen (bonded oxygen, dissolved oxygen, etc.) is used to catalyze silver cations (Ag ⁺ ). By converting into active oxygen and exerts bactericidal action, the superoxide dimutase (SOD) is added to it, and the basis for the antimicrobial activity is lost. Third, antimicrobial and bactericidal action as a metal can be mentioned. Unlike other metals and silver, nano silver is nanoscaled to a small size of less than 10 nm, so microorganisms of about 100 nm to 200 nm are ingested. It can be estimated that the bacteria die due to respiratory and metabolic disorders when ingested. In antimicrobial agents using other inorganic carriers, antimicrobial metal ions (Ag ⁺ , Zm ²⁺ , Cu ²⁺ ) dissociated from inorganic carriers are believed to inhibit cellular energy metabolism by binding to proteins such as cell membranes and enzymes. The evidence can be explained by the transfer of silver cations (Ag ⁺ ) into the cells. However, the size of silver particles is 2nm ~ 10nm or less, microorganisms can be considered to have a sterilizing power as a metal itself causing metabolic disorders when ingested.

The nano silver is preferably used in the particle size of the range of 5 to 20nm, 50 to 1000ppm nanosilver of 1 to 5% by weight, preferably 3 to 5% by weight relative to the antimicrobial composition. In the following ranges, the lowering of the antimicrobial effect occurs, and the above-mentioned range is preferable because the compatibility problems such as precipitation due to lowering the solubility is included.

On the other hand, aromatherapy with essential oils has been developed since ancient medicines, leaves, roots, and stems have been found in ancient Egypt, China, India, etc., which have beneficial effects on the management of the human mind and body. It can be said that it is the stem of various systems. Essential oils play an important role in the life cycle and germination process of the plant itself, and have the ability to fight disease, which has been described as a plant's hormone or vitality, and has been used for religious consciousness, wound healing and beauty.

Eucalyptus, German chamomile, cedarwood, cinnamon, bergamot, lemon, cypress, lemongrass, juniper berry, tea tree, peppermint, rosemary, sandalwood, ginger, which are known to have antibacterial and anti-inflammatory effects among natural essential oils , Lavender and thyme have long been used in skin diseases in the West due to their excellent antibacterial and anti-inflammatory properties.

Eucalyptus is a tall evergreen tree of the foliar family. It is an oil extracted from the leaves of the most native tree in Australia and has analgesic, antiviral and antibacterial effects.

German chamomile is an oil derived from the petals of the asteraceae native to the Mediterranean coast and has been used as a home medicine for more than 2000 years in Europe. It contains antibacterial and anti-inflammatory azulene ingredients, which are effective for skin irritation such as acne, eczema, diaper eczema, and burns.

Cedarwood is an oil extracted from the conifers of the Cypressaceae family and used as an antiseptic and cosmetics in ancient Egyptian times. Its scent has the ability to fight off ants, moths and other harmful insects. have.

Cinnamon is an antibacterial oil derived from the leaves and branches of tropical camphoraceae. It has antibacterial activity against most bacteria.

Bergamot is an oil extracted from peels such as citrus fruits of the fragrance and is effective in combating eczema, dry swelling, acne, seborrhea of the skin and scalp, chicken pox, shingles and insects.

Lemon is an oil extracted from the tangerine peel and has antiseptic, antibacterial and antioxidant effects, and is known to inactivate at 0.2% dilution against Mycobacterium tuberculosis.

Cypress is an oil extracted from the leaves of the evergreen tree of the Cypressaceae.

Lemongrass is an oil derived from the leaves of the poaceae and has antimicrobial, antioxidant and antifungal effects.

Juniper berry is an oil extracted from the fruit of the evergreen conifer of Thymeaceae. It has antibacterial and disinfecting effect and is used for lotion after shaving.

Tea tree is an oil extracted from the leaves and twigs of the small-leaved pods. It is known to be active against anti-bacterial, antibacterial and anti-viral disinfection.

Peppermint is an oil extracted from the leaves and flowers of the perennial herb. It is effective for bronchial diseases and has anti-inflammatory, antibacterial, anti-inflammatory and antiviral effects.

Rosemary is an oil extracted from the flower part of the shrub and it has antibacterial, antifungal, antiseptic and insect repellent effects.

Sandalwood is an algae, known as sandalwood, and is an oil extracted after drying the core of a tree.

Ginger is an oil extracted from the dried roots of Ginger Herb and has antiseptic, antibacterial and antioxidant effects.

Lavender is a perennial shrub and is the most popular and widespread antimicrobial, soothing and anti-inflammatory activity.

Thyme is an evergreen tree from the perennial herb, and is an oil extracted from leaves and flowers.

The antimicrobial composition of the present invention, as mentioned above, eucalyptus, german chamomile, cedarwood, cinnamon, bergamot, lemon, cypress, lemongrass, juniper berry, tea tree, peppermint, rosemary, sandalwood, ginger, lavender, thyme Natural essential oils are selected by selecting at least one from the group consisting of, the amount thereof is preferably used in the range of 5 to 30% by weight, preferably 5 to 15% by weight. If it is used less than 5% by weight, there is a problem of lowering the antimicrobial activity, on the contrary, when used in excess of 30% by weight, the strength of the aroma fragrance is high, so there is a problem that causes rejection, so adding within the above range desirable.

The present invention will be described in more detail based on the following examples.

Example 1

As shown in the following 1, an antimicrobial composition consisting of grapefruit, machi and Echochocho extract and a concentration of 1000ppm nanosilver, and natural essential oils of cinnamon, tea tree and lemon were prepared. The base solution at this time was 1.3-butylene glycol: ethanol = 1: 2.

The antibacterial activity was conducted by a paper disc test (Kirby-Bauer Antimicrobial Sensitivity Test) method. First, in order to activate each strain was incubated 48 ~ 72 hours ago in the appropriate medium for each strain. The culture medium thus prepared was smeared and dried 0.1 ml each in the medium. 30 μl of the antimicrobial composition prepared above was taken and dipped onto a paper disc having a diameter of 8 mm to incubate under the conditions shown in Table 2 below. Table 3 shows the results (antibacterial effect) of measuring the diameter of the clear zone appearing around the paper disk after incubation.

Comparative Example 1

In order to observe the antimicrobial effect by the natural extract only was carried out in the same manner as in Example 1 except for preparing an antimicrobial composition in the composition and composition ratio as shown in Table 1. Table 3 shows the results (antibacterial effect) of measuring the diameter of a clear zone appearing around the paper disk.

Comparative Example 2

In order to observe the antimicrobial effect by the nanosilver only was carried out in the same manner as in Example 1 except for preparing an antimicrobial composition in the composition and composition ratio as shown in Table 1. Table 3 shows the results (antibacterial effect) of measuring the diameter of a clear zone appearing around the paper disk.

Comparative Example 3

In order to observe the antimicrobial effect by only the natural essential oil was carried out in the same manner as in Example 1 except for preparing an antimicrobial composition in the composition and composition ratio as shown in Table 1. Table 3 shows the results (antibacterial effect) of measuring the diameter of a clear zone appearing around the paper disk.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Grapefruit extract 0.5 0.5 - - March extract 0.5 0.5 - - Eoseongcho Extract 0.5 0.5 - - Nano silver One - One - Cinnamon 5 - - 5 Tea tree 3 - - 3 lemon 3 - - 3

(Unit: weight%)

number host badge Time / temperature condition One Candida albicans ATCC10259 Agar Badge 48 hours, 37 ℃ Exhalation 2 Escherichia coli (ATCC 25922) Blood agar medium 48 hours, 37 ℃ Exhalation 3 Staphylococcus aureus ATCC29737 Agar Badge 48 hours, 37 ℃ Exhalation 4 Bacillus subtilis ATCC6633 Agar Badge 48 hours, 37 ℃ Exhalation 5 Straptococcus mutans ATCC25175 Liquid medium 24 hours, 37 ℃ Exhalation 6 Staphylococcus epidermidis (Staphylococcus epodermidis ATCC12228) Agar Badge 48 hours, 37 ℃ Exhalation 7 Acne bacteria (Propionibaterium acne ATCC6919) RCM 72 hours, 37 ℃ anaerobe

※ RCM: Reinforced clostridial agar medium

Classification (mm) Use strain (number of table 2) One 2 3 4 5 6 7 Example 1 27 26 28 25 27 25 26 Comparative Example 1 11 12 11 12 12 13 11 Comparative Example 2 17 19 21 20 22 18 19 Comparative Example 3 15 14 19 15 21 17 16

As shown in Example 1, the area of the clean zone is wider than that of Comparative Example 1, Comparative Example 2, and Comparative Example 3, and thus, it can be seen that it is superior to the antibacterial activity of natural extracts, nanosilver, and essential oil, respectively. It can be seen that these have synergistic effects with each other and exert an excellent antimicrobial effect.

Preparation Example 1

Hand cleaners were prepared in the following composition. Table 4 shows the results of measuring the antimicrobial effect on the prepared antimicrobial hand cleaner.

ingredient usage Grapefruit extract 0.1 wt% Machi Prefecture Extract 0.1 wt% Eoseongcho Extract 0.1 wt% Nano silver 1.0 wt% Cinnamon 3 wt% Tea tree 1 wt% lavender 1 wt% Camomer 0.5 wt% ethanol 50 wt% TEA 0.34 wt% antiseptic a very small amount Distilled water Remaining amount

Comparative Production Example 1

A hand cleaner having antimicrobial properties was prepared as follows. The antimicrobial effect of the antimicrobial hand cleaner is shown in Table 4 below.

ingredient usage Grapefruit extract 0.1 wt% Machi Prefecture Extract 0.1 wt% Eoseongcho Extract 0.5 wt% Nano silver - Cinnamon - Tea tree - lavender - Camomer 0.5 wt% ethanol 50 wt% TEA 0.34 wt% antiseptic a very small amount Distilled water Remaining amount

Comparative Production Example 2

A hand cleaner having antimicrobial properties was prepared as follows. The antimicrobial effect of the antimicrobial hand cleaner is shown in Table 4 below.

ingredient usage Grapefruit extract - Machi Prefecture Extract - Eoseongcho Extract - Nano silver 1.0 wt% Cinnamon - Tea tree - lavender - Camomer 0.5 wt% ethanol 50 wt% TEA 0.34 wt% antiseptic a very small amount Distilled water Remaining amount

Comparative Production Example 3

A hand cleaner having antimicrobial properties was prepared as follows. The antimicrobial effect of the antimicrobial hand cleaner is shown in Table 4 below.

ingredient usage Grapefruit extract - Machi Prefecture Extract - Eoseongcho Extract - Nano silver - Cinnamon 3 wt% Tea tree 1 wt% lavender 1 wt% Camomer 0.5 wt% ethanol 50 wt% TEA 0.34 wt% antiseptic a very small amount Distilled water Remaining amount

Classification (mm) Use strain (number of table 2) One 2 3 4 5 6 7 Example 1 25 28 24 25 24 21 23 Comparative Example 1 9 12 10 9 9 10 9 Comparative Example 2 14 16 18 19 17 17 16 Comparative Example 3 14 16 17 14 19 17 15

Antimicrobial activity test results of Preparation Example 1, Comparative Preparation Example 1, Comparative Preparation Example 2, Comparative Preparation Example 3 as a hand-cleaning moisturizing agent, it was found that the antibacterial synergistic effect of natural extracts and nano silver and natural essential oils is increased.

The antimicrobial composition of the present invention can be applied to various products as follows.

Preparation Example 2

Aroma fragrances having antimicrobial properties were prepared in the following compositions.

ingredient usage Octyldodecanol 10 wt% Rosemary 5 wt% time 7 wt% Sandalwood 3 wt% Fiji-60 Hydrogenated Castor Oil 0.5 wt% Benzophenone-3 0.05 wt% Nano Silver (1000 ppm) 0.5 wt% Distilled water 5 wt% ethanol Residual weight%

Preparation Example 3

An antimicrobial herb gel was prepared in the following composition.

ingredient usage Pyromethicone 0.1 wt% Trimethylsiloxysilicate 0.1 wt% Polyethylene Oxide Propylene Oxide Monoallyl Ether Acetate 0.1 wt% Nano Silver (1000 ppm) 1.0 wt% Grapefruit extract 3 wt% Machi Prefecture Extract 1 wt% Eochocho extract 1 wt% ginger 0.5 wt% Lemongrass 50 wt% Peppermint 0.34 wt% Octyldodecanol a very small amount ethanol Remaining amount

The antimicrobial composition according to the present invention exhibits excellent antimicrobial activity against bacteria and fungi such as Staphylococcus aureus, skin flora, and skin filamentous fungi, and is suitable for preparing formulations of hand cleaners, aromatic fragrances, and herbal gels containing the same. Do.

Claims (4)

10 to 30% by weight of the natural extract consisting of a mixture of grapefruit, machi and eocho vinegar in a weight ratio of 1: 1: 1 to 5% by weight of nanosilver with a concentration of 50 to 1000 ppm, 5 to 30% by weight of one or more natural essential oil mixtures And the remainder is made of ethanol, characterized in that the antimicrobial composition containing natural extracts, nano silver and natural essential oils. According to claim 1, wherein the natural extract is 1 to 5% by weight, the concentration is 50 to 1000 ppm nano silver 3 to 5% by weight and natural essential oils, characterized in that 5 to 15% by weight natural extract, nano Antimicrobial composition containing silver and natural essential oils. The antimicrobial composition of claim 1 or 2, wherein the nanosilver particles have a size of 5 to 20 nm. The method of claim 1, wherein the natural essential oils are eucalyptus, German chamomile, cedarwood, cinnamon, bergamot, lemon, cypress, lemongrass, juniper berry, tea tree, peppermint, rosemary, sandalwood, ginger, lavender , Antimicrobial composition containing natural extract, nanosilver and natural essential oil, characterized in that at least one selected from the group consisting of thyme.