KR20050025831A - 유전자 변형 농작물의 검출 키트 - Google Patents
유전자 변형 농작물의 검출 키트 Download PDFInfo
- Publication number
- KR20050025831A KR20050025831A KR1020030062772A KR20030062772A KR20050025831A KR 20050025831 A KR20050025831 A KR 20050025831A KR 1020030062772 A KR1020030062772 A KR 1020030062772A KR 20030062772 A KR20030062772 A KR 20030062772A KR 20050025831 A KR20050025831 A KR 20050025831A
- Authority
- KR
- South Korea
- Prior art keywords
- epsps
- conjugate
- antigen
- genetically modified
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000009007 Diagnostic Kit Methods 0.000 title abstract 3
- 239000000427 antigen Substances 0.000 claims abstract description 38
- 102000036639 antigens Human genes 0.000 claims abstract description 38
- 108091007433 antigens Proteins 0.000 claims abstract description 38
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 claims abstract description 36
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000012086 standard solution Substances 0.000 claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 239000011148 porous material Substances 0.000 claims abstract description 6
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 25
- 244000037671 genetically modified crops Species 0.000 claims description 19
- 239000000523 sample Substances 0.000 claims description 14
- 239000012488 sample solution Substances 0.000 claims description 14
- 239000000084 colloidal system Substances 0.000 claims description 13
- 239000010409 thin film Substances 0.000 claims description 12
- 238000011161 development Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000009739 binding Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 230000009261 transgenic effect Effects 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 17
- 235000003869 genetically modified organism Nutrition 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 230000001295 genetical effect Effects 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 235000013617 genetically modified food Nutrition 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 235000013305 food Nutrition 0.000 description 6
- 235000021067 refined food Nutrition 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- ZBKIUFWVEIBQRT-UHFFFAOYSA-N gold(1+) Chemical compound [Au+] ZBKIUFWVEIBQRT-UHFFFAOYSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 3
- 102000009027 Albumins Human genes 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 239000004009 herbicide Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 230000005875 antibody response Effects 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241000589196 Sinorhizobium meliloti Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020030062772A KR20050025831A (ko) | 2003-09-08 | 2003-09-08 | 유전자 변형 농작물의 검출 키트 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020030062772A KR20050025831A (ko) | 2003-09-08 | 2003-09-08 | 유전자 변형 농작물의 검출 키트 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20050025831A true KR20050025831A (ko) | 2005-03-14 |
Family
ID=37383983
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020030062772A Ceased KR20050025831A (ko) | 2003-09-08 | 2003-09-08 | 유전자 변형 농작물의 검출 키트 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR20050025831A (ko) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103792370A (zh) * | 2014-02-13 | 2014-05-14 | 中国检验检疫科学研究院 | 利用QCM传感器检测CP4-EPSPs蛋白的方法及专用金片 |
CN103792371A (zh) * | 2014-02-13 | 2014-05-14 | 中国检验检疫科学研究院 | 利用表面等离子共振传感器检测抗草甘膦cp4-epsps蛋白的方法 |
-
2003
- 2003-09-08 KR KR1020030062772A patent/KR20050025831A/ko not_active Ceased
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103792370A (zh) * | 2014-02-13 | 2014-05-14 | 中国检验检疫科学研究院 | 利用QCM传感器检测CP4-EPSPs蛋白的方法及专用金片 |
CN103792371A (zh) * | 2014-02-13 | 2014-05-14 | 中国检验检疫科学研究院 | 利用表面等离子共振传感器检测抗草甘膦cp4-epsps蛋白的方法 |
CN103792371B (zh) * | 2014-02-13 | 2016-01-06 | 中国检验检疫科学研究院 | 利用表面等离子共振传感器检测抗草甘膦cp4-epsps蛋白的方法 |
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Legal Events
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A201 | Request for examination | ||
PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20030908 |
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PA0201 | Request for examination | ||
PG1501 | Laying open of application | ||
E902 | Notification of reason for refusal | ||
PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20051109 Patent event code: PE09021S01D |
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E601 | Decision to refuse application | ||
PE0601 | Decision on rejection of patent |
Patent event date: 20060525 Comment text: Decision to Refuse Application Patent event code: PE06012S01D Patent event date: 20051109 Comment text: Notification of reason for refusal Patent event code: PE06011S01I |