KR20020012391A - Manufacturing method and composition of fruit-body or mycelial mat of medicinal mushroom - Google Patents

Abstract

PURPOSE: Provided are a processing method of a fruit body or a mycelium of medicinal mushrooms to maintain its natural active ingredients and a composition using the same. CONSTITUTION: The fruit body or mycelium of medicinal mushrooms is processed to solids by adding a functional composition thereto then freeze-drying them for 4-48 hours under vacuum condition. The mushroom is selected from Cordyceps sp., Phellinus linteus, Armillaria sp., Lentinus edodes, Tricholoma matsutake, Inonotus obliquus., Ganoderma lucidum., Agaricus blazei, Coriolus so., and Grifola sp., and the functional composition is selected from: a) a food additive selected from glucose, maltose, galactose, lactose, sucrose, caramel and yeast extract; b) at least one of calcium carbonate, calcium gluconate, calcium lactate and vitamin D3; c) a medicinal herb selected from the group consisting of jujube, licorice root, Acanthopanax senticosus Harms, Cornus officinalis S. et Z, Angelica sinensis (Oliv) Diels, Polygonatum odoratum, Rosa rugosa, pine needle, Camellia sinensis, Codonopsis lanceolata, Lycii Fructus, Pueraria thunbergiana benth, Platycodon grandiflorum, acacia and buckwheat; d) a herb plant selected from the group consisting of rosemary, lavender, lemon grass, peppermint, lemon thyme, sage, lemon verbena, golden thyme, teatree, and pineapple sage; and e) soluble chitosan.

Description

Manufacturing method and composition of fruit-body or mycelial mat of medicinal mushroom}

The present invention relates to a method for processing mushrooms, and more particularly, to a method for preparing a water-soluble solid by adding a functional composition to a liquid extract which is an alcohol or hot water extract of various mushrooms.

In general, the use of mushrooms is used to take boiled extracts or boiled fruit as food. According to "a method for preparing tea and beverages using the situation mushrooms or mushroom fungus mycelium of Patent Publication 99-031," the cultured mycelium was juiced, filtered, concentrated and sterilized, and then sweetener was added. However, the present invention is distinguished from liquid extracts by adding hot water or alcohol extracted extracts to water-soluble solids to prepare the composition.

Anticancer ability such as Cordycepin (Cordyceps), polysaccharides (Sea, Cordyceps, Ganoderma, Situation, Matsutake, Black Birch Mushroom, Agaricus, Cloudy, Herb) of mushrooms recently; As well as hypoglycemic ability (Cordyceps sinensis); Thrombolytic ability (Mulberry mushroom; aka Gaegum mushroom); Prevention or treatment of osteoporosis, such as vitamin D ₃ (shiitake mushroom); As adenosine (A Cordyceps sinensis) such as nourishing tonic ability is found to be excellent, drink products and extract products are commercialized. However, existing products may not be fully reinforced with the functional composition in the liquid state in which the mushrooms themselves are processed into powder or extracted extract.

An object of the present invention is to improve the functionality and at the same time make it easy for the general public to take the extract of various mushrooms without going through a complicated process.

The present invention is to extract the extract of mushrooms using alcohol or hot water to add the functional composition chitosan (diabetes prevention and nourishment effect), calcium, vitamin D ₃ (osteoporosis treatment or prevention effect), sweeteners, herbal medicines, herbs, etc. In addition to the inherent efficacy of the mushrooms themselves, the functionality of the product was improved, and at the same time liquid extract type was prepared in the form of a solid powder or granules or tablets. That is, the water soluble type of the water-soluble type obtained in the present invention is easy to carry and easy to store and transport for a long time, and can be easily dissolved in water at any time any time if only cold water or hot water can be prepared Has

The present invention is Cordyceps sp. , Cordyceps spp. , Phellinus linteus . , Mushroom mulberry fungus ( Armillaria sp. ), Shiitake mushroom ( Lentinus edodes. ), Pine mushroom ( Tricholoma matsutake ), Black birch mushroom One or more mushroom fruiting bodies selected from the group consisting of Chaga, Inonotus obliquus. , Ganoderma lucidum. , Agaricus blazei , Coriolus sp. , And Grifola sp. One or more food additives selected from the group consisting of glucose, maltose, galactose, lactose, sucrose, caramel and yeast extract; Calcium carbonate, calcium gluconate. One or more osteoporosis application ingredients selected from the group consisting of calcium lactate and vitamin D ₃ ; One or more medicinal herbs selected from the group consisting of jujube, licorice, ginger, cornus, round root, guava, periwinkle, Angelica, safflower seed, pine needle, pine pollen, brown root, gilyeong, acacia, buckwheat; One or more herbal plants selected from the group consisting of rosemary, lavender, lemongrass, sage, peppermint, lemontime, lemon verbena, golden thyme, tea treero, pineapple grass sage; And it is achieved by the mycelium and fruiting body processing method of the mushrooms, characterized in that the water-soluble chitosan is added to the composition to obtain a water-soluble solids through the drying process, processing into a granule type.

The specific processing method of this invention is demonstrated.

I. Extract extraction

1) Hot water extraction

Take mushroom mycelium and real or mycelium as a sample to prepare a fine powder using a grinding machine, prepare with sterile distilled water 1: 1 to 1: 5 (V /%) and then 130 ℃ for 10 minutes to 180 minutes in a shaker The liquid extract was obtained by applying a high temperature of 1 to 3 kg / cm 3. At this time, if the sample is completely dried, immersion in sterile distilled water of 45 ~ 70 ℃ about 4 hours in advance so that the moisture is sufficiently wet. After the extraction is completed, the mixture is cooled slowly at room temperature to allow precipitation to take place, and then only the supernatant is removed to remove the precipitate, and the impurities are removed by using a filter of about 0.2 micropore to obtain a pure solution. If the precipitate is left for about 48 hours in the refrigerator at 4 ℃, repeat the process of removing impurities.

2) alcohol extraction

Using a 70-80% ethanol, methanol or butanol, put a fine powder sample into a round glass flask so that 15-65% (V / V) is immersed in warm water at 40-50 ℃, and slowly stirring it for 3-12 hours. Elute components After the elution is completed, the impurities are removed in the same manner as in the case of “I-1), and then dried using a vacuum distillation apparatus at 65 to 80 ° C. until the alcohol component is completely evaporated. After drying is complete, the resulting crystals are collected and sterile distilled water is added to obtain a dissolved liquid extract. At this time, do not dissolve the material that is precipitated, do not use only the supernatant, and if it is left for more than 48 hours in the refrigerated state (4 ℃), if a precipitate is formed, remove impurities in the same manner as described in "I-1)". Go through the process.

II. Composition preparation

1) Formulation of Group I

Prepare one or more of glucose, maltose, galactose, lactose, sucrose, and caramel belonging to Group I by weighing about 10 to 75% (V /%) of the total amount extracted in the above "I". do.

2) Preparation of Group II Composition

One or more of calcium carbonate, calcium gluconate, and calcium lactate, which are calcium agents belonging to group II, are prepared in the same manner as in the above section "II-1).

3) Preparation of Group III Composition

Herbal medicine, pine pollen, pine needles, pine needles, pine stalks, rosin and pine roots belonging to group III shall be prepared in powder or carried out in parallel with hot water extraction in the above "I-1)" section. At this time, the composition addition rate is 0.5 to 38% (W / W) of the sample amount. And if the herbs are dried products absorb water enough for about 4 hours, if the raw materials as it is extracted juice using a green juice. If there is not enough water in the process of extracting the juice, an appropriate amount of sterile distilled water can be added to make herbal green juice. Then prepare the juice solution obtained through the filtering process to prevent the formation of sediment.

4) Preparation of Group IV Composition

A water-soluble chitosan, a composition of Group IV, was prepared by weighing 0.1-55% (V /%) of the extract extracted in the above section "I".

III. Solidification

When all the processes of "I" and "II" are completed, the composition prepared in "II" is mixed and dissolved in the extract prepared in "I". At this time, in order to fully melt | dissolve a composition, if temperature is maintained about 45 degreeC for 4 to 5 hours, stirring is performed gradually.

Subsequently, freeze-vacuum drying for 4 to 48 hours in a vacuum of about 10-2 to 3 torr to obtain a solid content to obtain a solid crystals. At this time, care should be taken so that the drying temperature does not exceed 65 ° C so as not to lose the efficacy and aroma of the functional ingredient. The crystallized solids are ground and used as granule-type powders, or the powders are used as tea or health food additives.

Solids produced by the present invention can be used in whole or in part as a therapeutic and preventive agent for tuberculosis, asthma, anticancer, antithrombotic, antiglycemic, immune enhancing, nourishing tonic, osteoporosis, alopecia, hypertension. And the present invention can be taken as tea or functional beverages dissolved in water as a health supplement food as a water-soluble solids, solids can be taken with water as it is.

Hereinafter will be described in more detail using an embodiment of the present invention.

Example 1 Extraction

2,500 ml of sterile distilled liquor dried powdered Cordyceps sinensis (4% W: V), green mushroom (2.5% W: V), green mushroom mycelium (2.0% W: V), pine mushroom (1.0% W: V), pine mycelium (1.2% W: V), shiitake mushroom (3.0% W: V), mulberry mycelium (2.5% W: V) was soaked for 4 hours to absorb water, and then in a shaker for 65 minutes to 120 ℃, 1.2 A kg / cm 3 pressure is applied to give an extract in the liquid state.

In order to remove the precipitate contained in the extracted extract was slowly cooled at room temperature, only the supernatant portion was taken and transferred to another container, impurities were removed using a 0.2 microporous filter to obtain 2,000 ml of pure extract. The container used here is made of heat-resistant glass or stainless steel container, and should not be used in plastic container.

Example 2 Preparation of Additive Composition

Glucose (32.4% W: V), Calcium Carbonate (3.0% W: V), 20.0% (W: V) pine needle hot water extract (10.0% V: V), 45% (W: V) licorice and jujube, Cornus hot water extract (12.0% V: V), 30% (W: V) lemongrass and lemon juice (1.5% V: V), water-soluble chitosan powder (1.45% W: V) was prepared.

Example 3 Solidification

1) In order to mix and dissolve the extract prepared in "Example 1" and the additive composition prepared in "Example 2", stirring was performed for 4 hours in a constant temperature of 45 degreeC.

2) To obtain a solid, freeze-dried for 12 hours at minus 65 ℃ in a vacuum of 10-2 toru to obtain a solid crystals. The obtained solid content was 730g, and the yield was 36.50%.

Example 4 Comparative Test of Solubility of Mushroom Tea by Samples

The water-soluble mushroom tea solids of the present invention obtained in Example 3 and the conventional products in the form of conventional solids were each weighed 10g each and placed in a 500ml beaker and 100ml of sterile water was added. Stirred for 30 minutes at the same stirring speed (about 25 ± 1 ℃ at room temperature) in a stirrer. The sample that had been sufficiently dissolved for 30 minutes was filtered (filter 90mm / 5C). The filtered filter paper was collected and dried overnight in a 50 ° C. dryer (8 hours) to measure the weight of the pure pellet that had been sufficiently dehydrated. Was compared.

The results are published in Table 1 below.

Table 1

Sample Name Measures Remarks Newly developed water soluble mushroom tea 0.02 g Solubility rate (%) = 99.8 Existing Products in Solid Form 7.64 g Solubility Rate (%) = 23.6

※ Solubility rate formula: Measured value (g) ÷ sample weight (g) X 100 ---- ①

100-① = Solubility rate (%)

The sample obtained in the present invention had a solubility of about 4.2 times higher than that of the conventional solid mushroom form, and was excellent as a water-soluble mushroom tea.

As can be seen in the above embodiment, the present invention is a water-soluble solid, so that it can be taken as a health supplement food dissolved in water or tea or functional beverage. That is, it is easier to carry than a drink or extract type product, and there is no fear of deterioration even after long-term storage.

And the calcium agent in the functional composition of the present invention has an osteoporosis treatment and prevention effect, chitosan component has a nourishing tonic effect and immune enhancing effect.

In addition, the mushrooms of the present invention not only contain anti-cancer effect, lower blood sugar, improve immunity, lower blood pressure, supply vitamin D ₃ , but also use ingredients rich in flavonoid-based ingredients in Chinese medicine, so the adenosine contained in mushrooms Helps to improve stamina, coronary artery disease, fatigue and stress relief.

Claims (5)

Cordyceps sp. , Cordyceps sp. , Phellinus linteus. , Armillaria sp. , Shiitake mushroom ( Lentinus edodes. ), Matsutake mushroom ( Tricholoma matsutake ), Black birch mushroom ( Agar, Chaga , Inonotus obliquus. , Ganoderma lucidum.Agaricus mushroom, Agaricus blazei , Coriolus sp. , And mushrooms of Grifola sp. (A) one or more food additives selected from glucose, maltose, galactose, lactose, sucrose, caramel, and yeast extract; (b) one or more osteoporosis application ingredients selected from calcium carbonate, calcium gluconate, calcium lactate, and vitamin D ₃ ; (c) jujube, licorice, thorny goose, cornus, donkey, roundcorn, guava, perilla, pine needles, tea tree One or more herbal medicines selected from the group deodeok, wolfberry, brown root, gilkyung, acacia, buckwheat; (d) one or more herbal plants selected from the group of rosemary, lavender, lemongrass, sage, peppermint, lemon thyme, lemon verbena, golden thyme, tea tree, pineapple grass sage; and (e) adding at least one functional composition selected from the group consisting of water-soluble chitosan and freeze-vacuum drying for 4 to 48 hours in a vacuum state of 10-2 to 3 torr to produce solids. The method of claim 1, wherein the drying is performed at a temperature of 65 ° C. or lower in the freeze vacuum drying process. The method of claim 1, wherein the water-soluble solid is molded into a powder, granule or tablet. Cordyceps sp. , Cordyceps sp. , Phellinus linteus. , Armillaria sp. , Shiitake mushroom ( Lentinus edodes. ), Matsutake mushroom ( Tricholoma matsutake ), Black birch mushroom ( Agar, Chaga , Inonotus obliquus. , Ganoderma lucidum.Agaricus mushroom, Agaricus blazei , Coriolus sp. , And mushrooms of Grifola sp. And (a) one or more food additives selected from glucose, maltose, galactose, lactose, sucrose, caramel, and yeast extract; (b) one or more osteoporosis application ingredients selected from calcium carbonate, calcium gluconate, calcium lactate and vitamin D ₃ ; One or more herbal medicines selected from the group deodeok, wolfberry, brown root, gilkyung, acacia, buckwheat; (d) one or more herbal plants selected from the group of rosemary, lavender, lemongrass, sage, peppermint, lemon thyme, lemon verbena, golden thyme, tea tree, pineapple grass sage; A dietary supplement comprising solids obtained by hot air or freeze-vacuum drying of at least one functional composition selected from the group consisting of (e) and water-soluble chitosan. 5. The dietary supplement of claim 4 wherein the water soluble solid has a powder, granule or tablet form.