KR100385739B1 - Manufacturing method and composition of fruit-body or mycelial mat of medicinal mushroom - Google Patents
Manufacturing method and composition of fruit-body or mycelial mat of medicinal mushroom Download PDFInfo
- Publication number
- KR100385739B1 KR100385739B1 KR10-2000-0045669A KR20000045669A KR100385739B1 KR 100385739 B1 KR100385739 B1 KR 100385739B1 KR 20000045669 A KR20000045669 A KR 20000045669A KR 100385739 B1 KR100385739 B1 KR 100385739B1
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- KR
- South Korea
- Prior art keywords
- mushroom
- water
- cordyceps
- mushrooms
- mycelium
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/306—Foods, ingredients or supplements having a functional effect on health having an effect on bone mass, e.g. osteoporosis prevention
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/308—Foods, ingredients or supplements having a functional effect on health having an effect on cancer prevention
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/208—Fungi extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
- A23V2250/511—Chitin, chitosan
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
본 발명은 기능성 버섯의 균사체 또는 자실체, 균핵추출물을 천연 유효성분이 그대로 유지되도록 하고, 복용하기 편리하도록 함과 동시에 기능성 조성물을 첨가하여 효능을 높인 가공방법에 관한 것이다.The present invention relates to a processing method of increasing the efficacy by adding a functional composition to the mycelium or fruiting body of the functional mushroom, so as to maintain the natural active ingredient intact, and convenient to take.
구체적으로 본 발명은 자낭균류 중 동충하초(Cordyceps sp.), 담자균류 중 상황버섯(Phellinus linteus.), 뽕나무버섯 속균(Armillaria sp.), 표고버섯(Lentinus edodes.), 송이버섯(Tricholoma matsutake), 검은자작나무버섯(일명; 챠가버섯;Inonotus obliqua.), 영지버섯(Ganoderma lucidum.) 아가리쿠스버섯(Agaricus blazei), 운지버섯(Coriolus sp.), 및 저령(Grifola sp.)으로 구성된 그룹으로부터 선택된 하나 이상의 버섯의 자실체 또는 균사체, 균핵에서 액체상태의 엑기스 추출물에 기능성 조성물을 첨가하여 분말 또는 그레뉼 형태나 타블렛 형태의 수용성 고형분으로 재결정시켜, 온수는 물론 냉수에서도 쉽게 용해 될 수 있도록 가공하는 것을 특징으로 한다.More specifically, the present invention is Cordyceps (Cordyceps sp.), Phellinus linteus (Phellinus linteus.), Mulberry mushroom spp (Armillaria sp.), Shiitake (Lentinus edodes.), Matsutake mushroom (Tricholoma matsutake) of the Basidiomycetes of the Ascomycetes, Black birch mushroom (aka; chaga mushroom; Inonotus obliqua. ), Ganoderma lucidum. Agaricus blazei , Coriolus sp. , And Grifola sp. A functional composition is added to the liquid extract from the fruiting body or mycelium of the at least one mushroom or mycelium, and recrystallized into a water-soluble solid in powder or granule form or tablet form, so that it can be easily dissolved in hot water or cold water. It is done.
Description
본 발명은 버섯류의 가공방법에 관한 것으로, 상세하게는 각종 버섯류의 알코올 또는 열수추출물인 액체 상태의 엑기스에 기능성 조성물을 첨가하여 수용해성 고형분으로 제조하는 방법에 관한 것이다.The present invention relates to a method for processing mushrooms, and more particularly, to a method for preparing a water-soluble solid by adding a functional composition to a liquid extract which is an alcohol or hot water extract of various mushrooms.
일반적으로 버섯류의 이용은 끓여서 우려낸 추출물을 복용하거나 자실체를 그대로 음식으로 만들어 이용되고 있다. "특허공보 99-031의 상황버섯 또는 신령버섯 균사체를 이용한 차 및 음료의 제조방법" 의하면 배양된 균사체를 착즙하여 여과후 농축시켜 살균한 후 감미료를 첨가하였다. 그러나 본 발명은 열수 또는 알콜 추출한 추출액을 수용해성 고형분으로 조성물을 가하여 조제함으로 액체 상태의 엑기스와는 구분된다.In general, the use of mushrooms is used to take boiled extracts or boiled fruit as food. According to "a method for preparing tea and beverages using the situation mushrooms or mushroom fungus mycelium of Patent Publication 99-031," the cultured mycelium was juiced, filtered, concentrated and sterilized, and then sweetener was added. However, the present invention is distinguished from the liquid extract by adding a composition with water-soluble solids to extract the hot water or alcohol extracted extract.
최근 버섯류가 가지고 있는 코르디세핀(Cordycepin)(동충하초), 다당류(표고, 동충하초, 영지, 상황, 송이, 검은자작나무버섯, 아가리쿠스, 운지, 저령) 등의 항암능력; 뿐만 아니라, 혈당저하능력(동충하초); 혈전용해 능력 (뽕나무버섯; 일명 개금버섯); 비타민 D3(표고버섯) 등의 골다공증에 관한 예방 또는 치료능력; 아데노신(Adenosine)(동충하초) 등 자양강장능력과 같은 우수한 효능이 밝혀지면서 드링크 제품이나 엑기스 제품이 상품화되고 있다. 그러나 기존 제품은 버섯 자체를 그대로 마쇠하여 분말로 가공하거나 엑기스를 추출한 액체상태로 기능성 조성물이 충분히 보강되어 있지 못하다.Anticancer ability such as Cordycepin (Cordyceps) and polysaccharides (Sea, Cordyceps, Ganoderma, Situation, Matsutake, Black Birch Mushroom, Agaricus, Cloudy, Herb) which mushrooms have recently; As well as hypoglycemic ability (Cordyceps sinensis); Thrombolytic ability (Mulberry mushroom; aka Gamgum mushroom); Prevention or treatment of osteoporosis, such as vitamin D 3 (shiitake mushroom); With the emergence of excellent efficacy such as adenosine (A Cordyceps sinensis) and nourishing tonic ability, drink products and extract products are commercialized. However, existing products may not be fully reinforced with the functional composition in the liquid state in which the mushrooms themselves are processed into powder or extracted extract.
본 발명의 목적은 각종 버섯류의 추출물을 복용함에 있어 일반인이 복잡한 과정을 거치지 않고 손쉽게 이용할 수 있도록 함과 동시에 그 기능성을 향상시키는 것이다.An object of the present invention is to improve the functionality and at the same time make it easy for the general public to take the extract of various mushrooms without going through a complicated process.
본 발명은 알코올 또는 열수를 이용, 추출한 버섯류의 엑기스를 기능성 조성물인 키토산(당뇨예방 및 자양강장 효과), 칼슘, 비타민 D3(골다공증 치료 또는 예방 효과), 감미료, 한방약재, 허브 등을 첨가하여 버섯류 자체가 가진 고유의 효능과 함께 상기 산물의 기능성을 향상시키고, 동시에 액체상태의 엑기스 타입을 고체상태의 분말 또는 그레뉼 이나 타블렛 형태로 조제할 수 있도록 하였다. 즉, 본 발명에서 얻어진 수용해성 고형분(water soluble type)의 제형은 휴대가 간편함과 동시에 장기간 저장 및 수송이 용이하고, 냉수 또는 온수만 준비할 수 있으면 언제 어느 때나 쉽게 물에 녹여 복용할 수 있는 장점을 갖는다.The present invention is to extract the extract of mushrooms using alcohol or hot water to add the functional composition chitosan (diabetes prevention and nourishment effect), calcium, vitamin D 3 (osteoporosis treatment or prevention effect), sweeteners, herbal medicines, herbs, etc. In addition to the inherent efficacy of the mushrooms themselves, the functionality of the product was improved, and at the same time liquid extract type was prepared in the form of a solid powder or granules or tablets. That is, the water soluble type of the water-soluble type obtained in the present invention is easy to carry and easy to store and transport for a long time, and can be easily dissolved in water at any time any time if only cold water or hot water can be prepared Has
상기 본 발명은 동충하초(Cordyceps sp.), 담자균류 중 상황버섯(Phellinus linteus.), 뽕나무버섯 속균(Armillaria sp.), 표고버섯 (Lentinus edodes.), 송이버섯(Tricholoma matsutake), 검은자작나무버섯(일명; 챠가버섯;Inonotus obliqua.), 영지버섯(Ganoderma lucidum.) 아가리쿠스버섯 (Agaricus blazei), 운지버섯(Coriolus sp.), 및 저령(Grifola sp.)으로 구성된 그룹에서 선택된 하나 이상의 버섯류 자실체 또는 균사체, 균핵으로부터 추출한 엑기스를 글루코즈, 말토즈, 갈락토즈, 락토즈, 슈크로즈, 캐러멜, 효모추출물로 구성된 그룹에서 선택된 하나 또는 그 이상의 식품첨가물; 탄산칼슘, 글루콘산칼슘. 젖산 칼슘 그리고 비타민 D3로 구성된 그룹에서 선택된 하나 또는 그 이상의 골다공증 적용성분; 대추, 감초, 생강, 산수유, 둥굴레 뿌리, 구아바, 해당화, 당귀, 홍화씨, 솔잎, 송화분, 갈근, 길경, 아카시아, 메밀로 구성된 그룹에서 선택된 하나 또는 그 이상의 한약재; 로즈마리, 라벤다, 레몬그라스, 세이지, 페퍼민트, 레몬타임, 레몬버베나, 골든타임, 티트리로, 파인애풀세이지 구성된 그룹에서 선택된 하나 또는 그 이상의 허브식물; 그리고 수용성 키토산을 조성물로 첨가하여 건조과정을 거쳐 수용해성 고형분을 획득, 그레뉼타입 타입으로 가공하는 것을 특징으로 하는 버섯류의 균사체 및 자실체 가공방법에 의해 달성된다.The present invention is Cordyceps sp. , Cordyceps spit fungi ( Phellinus linteus. ), Mulberry fungus ( Armillaria sp. ), Shiitake mushrooms ( Lentinus edodes. ), Pine mushrooms ( Tricholoma matsutake ), black birch mushroom (Aka; Chaga ; Inonotus obliqua. ), Ganoderma lucidum.Agaricus mushroom ( Agricus blazei ), Coriolus sp. , And Grifola sp. Or one or more food additives selected from the group consisting of glucose, maltose, galactose, lactose, sucrose, caramel and yeast extract; Calcium carbonate, calcium gluconate. One or more osteoporosis application ingredients selected from the group consisting of calcium lactate and vitamin D 3 ; One or more medicinal herbs selected from the group consisting of jujube, licorice, ginger, cornus, round root, guava, periwinkle, Angelica, safflower seed, pine needle, pine pollen, brown root, gilyeong, acacia, buckwheat; One or more herbal plants selected from the group consisting of rosemary, lavender, lemongrass, sage, peppermint, lemontime, lemon verbena, golden thyme, tea treero, pineapple grass sage; And it is achieved by the mycelium and fruiting body processing method of the mushrooms, characterized in that the water-soluble chitosan is added to the composition to obtain a water-soluble solids through the drying process, processing into a granule type.
본 발명의 구체적인 가공방법을 설명한다.The specific processing method of this invention is demonstrated.
Ⅰ. 엑기스 추출I. Extract extraction
1)열수추출1) Hot water extraction
버섯의 균사체 및 사실체 또는 균핵을 시료로 하여 마쇄기를 이용 미세한 분말로 조제하고, 멸균증류수와 1: 1∼1: 5(V/%)가 되도록 준비한 후 약탕기에서 10분∼180분 간 130℃의 고온과 1∼3㎏/㎤의 압력을 가하여 액체상태의 추출물을 얻는다. 이 때 시료가 완전히 건조된 경우 4시간 정도 45∼70℃의 멸균 증류수에 미리 침수시켜 수분이 충분히 젖도록 한다. 추출이 완료되면 상온에서 서서히 식히면서 침전이 일어나도록 한 후 상등액 부분만을 취하여 침전물을 제거한 후 0.2마이크로 포아 정도의 필터를 이용 불순물을 제거하여 순수한 액기스를 얻어낸다. 영상 4℃ 정도의 냉장 상태로 48시간 정도 방치하면서 침전물이 생길 경우 불순물 제거과정을 반복한다.Take mushroom mycelium and real or mycelium as a sample to prepare a fine powder using a grinding machine, prepare with sterile distilled water 1: 1 to 1: 5 (V /%) and then 130 ℃ for 10 minutes to 180 minutes in a shaker The liquid extract was obtained by applying a high temperature of 1 to 3 kg / cm 3. At this time, if the sample is completely dried, immersion in sterile distilled water of 45 ~ 70 ℃ about 4 hours in advance so that the moisture is sufficiently wet. After the extraction is completed, the mixture is cooled slowly at room temperature to allow precipitation to take place, and then only the supernatant is removed to remove the precipitate, and the impurities are removed by using a filter of about 0.2 micropore to obtain a pure solution. If the precipitate is left for about 48 hours in the refrigerator at 4 ℃, repeat the process of removing impurities.
2)알코올추출2) alcohol extraction
70-80%에탄올 또는 메탄올, 부탄올을 이용 미세한 분말시료를 15∼65%(V/V)되도록 둥근 유리플라스크에 넣고 40∼50℃의 온수에 침수시켜 천천히 교반하면서 3∼12 시간 동안 시료 속의 유효성분을 용출시키다. 용출이 완료되면 상기 "Ⅰ - 1)항"의 경우와 동일하게 불순물 제거 과정을 거친 후 감압 증류 장치를 이용 65∼80℃에서 알콜 성분이 완전히 증발될 때까지 건조시킨다. 건조가 완료된 후 생성된 결정체를 모아 멸균 증류수를 가하여 용해된 액체 상태의 엑기스를 얻는다. 이 때 용해되지 않고 침전되는 물질은 상등 액만을 취하여 사용하지 안도록 하고, 만약 냉장상태(4℃)로 48시간 이상 방치할 경우 침전물이 생기면 상기 "Ⅰ - 1)"항의 방법과 동일하게 불순물 제거과정을 거친다.Using a 70-80% ethanol, methanol or butanol, put a fine powder sample into a round glass flask so that 15-65% (V / V) is immersed in warm water at 40-50 ℃, and slowly stirring it for 3-12 hours. Elute components After the elution is completed, the impurities are removed in the same manner as in the case of “I-1), and then dried using a vacuum distillation apparatus at 65 to 80 ° C. until the alcohol component is completely evaporated. After drying is complete, the resulting crystals are collected and sterile distilled water is added to obtain a dissolved liquid extract. At this time, do not dissolve and precipitate the supernatant only. Do not use it. Go through the process.
Ⅱ. 조성물 조제II. Composition preparation
1) 그룹Ⅰ의 조성물 조제1) Formulation of Group I
그룹Ⅰ에 속하는 글루코즈, 말토즈, 갈락토즈, 락토즈, 슈크로즈, 카라멜 중 1종 또는 그 이상을 상기 "Ⅰ항"에서 추출된 엑기스 총량의 10∼75%(V/%) 정도 계량하여 준비한다.Prepare one or more of glucose, maltose, galactose, lactose, sucrose, and caramel belonging to Group I by weighing about 10 to 75% (V /%) of the total amount extracted in the above "I". do.
2) 그룹Ⅱ의 조성물 조제2) Preparation of Group II Composition
그룹 Ⅱ에 속하는 칼슘제인 탄산칼슘, 글루콘산칼슘, 젖산칼슘 중 1종 또는 그 이상을 상기 "Ⅱ - 1)"항과 같은 방법으로 동일한 비율로 준비한다.One or more of calcium carbonate, calcium gluconate, and calcium lactate, which are calcium agents belonging to group II, are prepared in the same manner as in the above section "II-1).
3) 그룹Ⅲ의 조성물 조제3) Preparation of Group III Composition
그룹 Ⅲ에 속하는 한약재와 송화분, 솔잎, 솔순, 소나무 줄기, 송진, 소나무 뿌리는 분말로 만들어 준비하거나 상기 "Ⅰ - 1)"항의 열수추출 시 병행하여 실시한다. 이때 조성물 첨가비율은 시료 량의 0.5∼38%(W/W)되게 한다. 그리고 허브류는 건조품일 경우 약 4시간 정도 충분히 수분을 흡수시키고, 생품일 경우 그대로 녹즙기를 이용 즙을 추출한다. 만약 즙을 추출하는 과정에서 수분이 부족할 경우 적당량의 멸균 증류수를 가하여 허브 녹즙을 만들 수 있다. 그리고 침전물이 생기지 않도록 하고 거르는 과정을 거쳐 얻어진 즙액을 준비한다.Herbal medicine, pine pollen, pine needles, pine needles, pine stalks, rosin and pine roots belonging to group III shall be prepared in powder or carried out in parallel with hot water extraction in the above "I-1)" section. At this time, the composition addition rate is 0.5 to 38% (W / W) of the sample amount. And if the herbs are dried products absorb water enough for about 4 hours, if the raw materials as it is extracted juice using a green juice. If there is not enough water in the process of extracting the juice, an appropriate amount of sterile distilled water can be added to make herbal green juice. Then prepare the juice solution obtained through the filtering process to prevent the formation of sediment.
4) 그룹Ⅳ의 조성물 조제4) Preparation of Group IV Composition
그룹Ⅳ의 조성물인 수용성 키토산을 상기 "Ⅰ항"에서 추출한 엑기스의 0.1∼55%(V/%)를 계량하여 준비한다.A water-soluble chitosan, a composition of Group IV, was prepared by weighing 0.1-55% (V /%) of the extract extracted in the above section "I".
Ⅲ. 고형화(固形化)III. Solidification
상기 "Ⅰ" 과 "Ⅱ"의 과정이 모두 완료되면 "Ⅰ"에서 준비된 엑기스에 "Ⅱ"에서 준비한 조성물을 혼합하여 용해시키다. 이때 조성물을 충분히 용해시키기 위하여 45℃ 정도의 온도를 4∼5시간 유지시키면 서서히 교반을 실시한다.When all the processes of "I" and "II" are completed, the composition prepared in "II" is mixed and dissolved in the extract prepared in "I". At this time, in order to fully melt | dissolve a composition, if temperature is maintained about 45 degreeC for 4 to 5 hours, stirring is performed gradually.
이어서 고형분을 획득하고자 10∼2torr정도의 진공상태에서 4∼48시간 냉동 진공 건조시켜 고형화된 결정체를 얻는다. 이때 기능성 성분의 효능과 향이 상실되지 않도록 건조 온도가 65℃ 이상을 넘지 않게 주의한다. 결정화된 고형분을 마쇠하여 그래뉼 타입의 분말로 이용하거나, 분말을 차 또는 건강식품 첨가물로 이용한다.Subsequently, freeze-vacuum drying for 4 to 48 hours in a vacuum of about 10 to 2 torr to obtain a solid content to obtain a solid crystals. At this time, care should be taken so that the drying temperature does not exceed 65 ° C so as not to lose the efficacy and aroma of the functional ingredient. The crystallized solids are ground and used as granule-type powders, or the powders are used as tea or health food additives.
본 발명에 의해 생산된 고형분은 결핵, 천식, 항암, 항혈전, 항혈당, 면역력 향상, 자양강장, 골다공증, 탈모증, 고혈압 등의 치료용 및 예방제제로 전체 또는 부분적으로 활용할 수 있다. 그리고 본 발명은 수용성 고형분임으로 건강보조 식품으로 물에 녹여 차나 기능성 음료로 복용할 수 있고, 고형분을 그대로 물과 함께 복용할 수 있다.Solids produced by the present invention can be used in whole or in part as a therapeutic and preventive agent for tuberculosis, asthma, anticancer, antithrombotic, antiglycemic, immune enhancing, nourishing tonic, osteoporosis, alopecia, hypertension. And the present invention can be taken as tea or functional beverages dissolved in water as a health supplement food as a water-soluble solids, solids can be taken with water as it is.
이하 본 발명의 실시 예를 사용하여 보다 상세하게 설명한다.Hereinafter will be described in more detail using an embodiment of the present invention.
실시예 1 엑기스 추출Example 1 Extraction
멸균증류주 2,500㎖에 건조 분말화된 동충하초(4% W: V ), 상황버섯(2.5% W : V), 상황 균사체(2.0% W : V), 송이버섯(1.0% W : V), 송이 균사체 (1.2% W : V), 표고버섯 (3.0% W : V ), 뽕나무버섯 균사체 (2.5% W : V)를 4시간 동안 침적시켜 수분을 흡수시킨 후 약탕기에서 120분 동안 65∼120℃, 1.2㎏/㎤의 압력을 가하여 액체 상태의 추출물을 얻는다.Dry powdered Cordyceps sinensis (4% W: V), green mushroom (2.5% W: V), green mycelium (2.0% W: V), matsutake mushroom (1.0% W: V), pine mycelium (1.2% W: V), shiitake mushroom (3.0% W: V), mulberry mycelium (2.5% W: V) was soaked for 4 hours to absorb water, and then in a shaker for 65 minutes to 120 ℃, 1.2 A kg / cm 3 pressure is applied to give an extract in the liquid state.
추출이 완료된 엑기스 중에 들어있는 침전물을 제거하기 위해 상온에서 서서히 식히면서 상등액 부분만을 취하여 다른 용기에 옮기고, 0.2마이크로 포아의 필터를 이용 불순물을 제거하고, 순수한 엑기스 2,000㎖를 얻어냈다. 이 때 사용되는 용기는 내열 유리 또는 스텐레스제 용기를 사용하며, 플라스틱 용기를 사용해서는 안 된다.In order to remove the precipitate contained in the extracted extract was slowly cooled at room temperature, only the supernatant portion was taken and transferred to another container, impurities were removed using a 0.2 microporous filter to obtain 2,000 ml of pure extract. The container used here is made of heat-resistant glass or stainless steel container, and should not be used in plastic container.
실시예 2 첨가 조성물의 조제Example 2 Preparation of Additive Composition
글루코즈 32.4g, 탄산 칼슘 3.0g, 솔잎 열수추출물(10.0% V : V) 20g, 감초와 대추, 산수유 열수추출물(12.0% V : V) 45g, 레몬그라스와 레몬타입 즙액(1.5% V : V) 30g, 수용성 키토산 분말 1.45g을 준비하였다.Glucose 32.4g, Calcium Carbonate 3.0g, Pine Leaf Hot Water Extract (10.0% V: V) 20g, Licorice and Jujube, Cornus Hot Water Extract (12.0% V: V) 45g, Lemongrass and Lemon Type Juice (1.5% V: V) 30 g and 1.45 g of water-soluble chitosan powders were prepared.
실시예 3 고형화(固形化)Example 3 Solidification
1) 상기 "실시예 1" 에서 준비한 엑기스 65ml에 "실시예 2"에서 준비한 첨가 조성물을 혼합하여 45℃의 항온기에 4시간 동한 교반을 실시하였다.1) The additive composition prepared in "Example 2" was mixed with 65 ml of the extract prepared in "Example 1", and stirring was performed for 4 hours in a 45 degreeC thermostat.
2) 고형분을 획득하고자 10∼2torr의 진공 상태에서 영하 65℃에서 12 시간 냉동진공건조시켜 고형화된 결정체를 얻는다. 이때 얻어진 고형분은 730g 으로 수득율은 36.50 % 였다.2) To obtain solids, freeze-drying for 12 hours at -65 ° C in a vacuum of 10 to 2 torr to obtain solidified crystals. The obtained solid content was 730g, and the yield was 36.50%.
실시예 4. 버섯차의 시료별 용해도 비교 실험Example 4 Comparative Test of Solubility of Mushroom Tea by Samples
실시예 3에서 얻은 본 발명의 수용성 버섯차 고형분과 종래의 고형분 형태의 기존 제품을 각각 10g씩 측량하여 500㎖비이커에 넣고 멸균수 100㎖를 넣었다.교반기에서 똑같은 교반속도로 30분간 교반(실온에서 약 25±1℃)하였다. 30분 동안 충분히 용해를 시킨 시료를 여과시켰다(여과지 90mm/5C) 여과된 여과지를 수거하여 50℃ 건조기에서 밤새(8시간) 건조시켜 충분히 수분이 제거된 순수 팰렛(pellet)의 무게를 측정하여 용해도를 비교하였다.The water-soluble mushroom tea solids of the present invention obtained in Example 3 and the conventional products in the form of conventional solids were each weighed 10 g each and placed in a 500 ml beaker, and 100 ml of sterile water was added. About 25 ± 1 ° C.). The sample that had been sufficiently dissolved for 30 minutes was filtered (filter 90mm / 5C). The filtered filter paper was collected and dried overnight in a 50 ° C. dryer (8 hours) to determine the weight of the pure pellet that had been sufficiently dehydrated. Was compared.
결과는 하기 표 1에 게시하였다.The results are published in Table 1 below.
본 발명에서 얻어진 시료는 기존 고형분 형태의 버섯 차에 비해 4.2배 정도로 용해도율이 높게 나타났으며, 수용성 버섯 차로 우수하였다.The sample obtained in the present invention had a solubility of about 4.2 times higher than that of the conventional solid mushroom form, and was excellent as a water-soluble mushroom tea.
상기 실시예에서 알수 있는 바와 같이 본 발명은 수용성 고형분이므로 건강보조 식품으로 물에 녹여 차나 기능성 음료로 복용할 수 있다. 즉, 드링크제나 엑기스형태의 제품보다 휴대가 간편하고, 장기간 저장하여도 변질될 염려가 없다.As can be seen in the above embodiment, the present invention is a water-soluble solid, so that it can be taken as a health supplement food dissolved in water or tea or functional beverage. That is, it is easier to carry than a drink or extract type product, and there is no fear of deterioration even after long-term storage.
그리고 본 발명의 기능성 조성물 중 칼슘제는 골다공증 치료 및 예방효과가 있으며, 키토산 성분은 자양강장 효과와 면역력 증강효과가 있다.And the calcium agent in the functional composition of the present invention has an osteoporosis treatment and prevention effect, chitosan component has a nourishing tonic effect and immune enhancing effect.
또한, 본 발명의 버섯류는 항암효과, 혈당저하, 면역력 향상, 혈압강하, 비타민 D3를 공급하는 성분이 함유되어 있을 뿐만 아니라 한약재 중에서 플라보노이드계 성분이 풍부한 재료를 사용하였으므로 버섯류에 함유된 아데노신의 체내흡수를 돕게되어 스태미너 향상, 관상동맥 관련 질병 및 피로회복, 스트레스해소에 도움을 준다.In addition, the mushrooms of the present invention not only contain anti-cancer effect, lower blood sugar, improve immunity, lower blood pressure, supply vitamin D 3 , but also use ingredients rich in flavonoid-based ingredients in Chinese medicine, so the adenosine contained in mushrooms Helps to improve stamina, coronary artery disease, fatigue and stress relief.
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