KR100608456B1 - Hangover Relief Composition Containing Complex Herbal Extracts of Palm Cactus, Alder, Root and Raven - Google Patents

Abstract

The present invention is a hangover relieve hangover containing a compound herbal extract consisting of Opuntia ficus indica , Alnus japonica STEUD., Pueraria lobata (WILLD.), OHWI and Hovenia dulcis THUNB. And relates to a functional food composition for the protection of the liver, the composition of the present invention has no side effects on the human body, so that the effect of alcohol decomposition and liver protection is excellent, the composition containing it as an active ingredient is healthy for the hangover and liver protection It can be usefully used as a functional food.

Hangover Relief, Complex Herb Extract, Health Functional Food

Description

Composition comprising complex herb extract of Opuntia ficus indica, Alnus japonica, Pueraria lobata and Hovenia dulcis showing hangover alleviating activity}             

1 is an oral dose of 3 mg / kg alcohol to an experimental animal, and then 30 minutes after oral administration of 8 g of the complex herbal extract of the present invention, and then blood alcohol concentration after 1, 2, 4, 6 and 8 hours of alcohol administration. Is a diagram representing

2 is an oral administration of 3 mg / kg of ethanol to an experimental animal, and then 30 minutes after oral administration of 8 g of the herbal extract of the present invention, and then 1, 2, 4, 6, 8 hours after administration of alcohol. A diagram showing the concentration of alcohol dehydrogenase (ADH) in the cytoplasm,

3 is an oral administration of 3 mg / kg of ethanol to an experimental animal, and then 30 minutes after oral administration of 8 g of the complex herbal extract of the present invention, and then 1, 2, 4, 6, 8 hours after administration of alcohol. Figure showing the concentration of aldehyde dehydrogenase (ALDH) in the cytoplasm.

The present invention relates to a functional food composition for the hangover and soy sauce protection containing a complex herbal extract consisting of palm cactus, alder, bark and reeds.

 Alcohol has not only been manufactured and consumed in human society for a long time, but it is also manufactured and sold in almost every country except the Islamic religion.

According to a survey conducted by 1,600 people at Samsung Hospital on the drinking culture of the workplace and health awareness of the liver, Koreans in their 20s and 50s drink about 87.1% of the alcoholic beverages 1 to 3 times a week. In terms of health, it is quite worrying.

As such, modern people consume excessive amounts of alcohol so much that they cannot digest it, causing side effects such as thirst, general boredom, fatigue, memory loss, bloating, indigestion, vomiting, diarrhea and vitamin deficiency. Many people suffer from the phenomenon, and the risk of alcoholism increases.

Normal alcohol metabolism is ingested by alcohols (ethyl alcohol, methyl alcohol, ethylene glycol, etc.), absorbed from the stomach or small intestine, 10% of which is excreted by breathing, sweating, urine, etc. Enters into blood vessels and is metabolized in the liver (M. Nakanishi; Saishin Igaku , 31 , p2086, 1976). Subsequently, alcohol dehydrogenase (ADH) present in hepatocytes oxidizes alcohol to acetaldehyde, and acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase in hepatocytes to muscle or adipose tissue throughout the body. It is finally decomposed into carbon dioxide and water. However, in this decomposition metabolic process, when alcohol metabolism abnormalities come due to excessive drinking, most suffer from hangovers such as heavy, painful, or sore throat due to un metabolized ethyl alcohol or acetaldehyde.

As part of the research on this phenomenon, compositions using choline acids, which are widely used as gallstone solubilizers, have been developed and marketed. These choline acids have an effect of edema, hepatic detoxification, hepatic blood flow improvement, fat absorption and fine action. Ursodeoxycholic acid, which is known to be effective in improving the liver function of chronic liver disease, systemic malaise due to hepatic dysfunction, indigestion, anorexia, physical fatigue due to biliary excretion, It is marketed as a gallstone dissolving agent, and is effective in treating fatty liver, and when administered orally, improvement of liver function and reduction of liver fat mass have been reported (Japanese Patent Laid-Open Publication No. 4-235918) Taurorusodeoxycholic acid ), And commercially available cheodeoxycholic acid and dehydrocholic acid as gallstone dissolving agents. .

However, the composition using choline acids is effective in general symptoms caused by stress such as increase of adrenal weight by stress and prevention of spleen atrophy, suppression of ascorbic acid content change of adrenal gland and suppression of blood biochemical changes. It is only proven and there is no mention of a hangover or liver protection effect.

Palm cactus ( Opuntia ficus indica ) is a perennial herb belonging to the Cactaceae family , which is native to Jeju, Korea. The stem and leaves contain triterpen, malic acid, and succinic acid. Its main effects are known to significantly reduce the hangover symptoms such as nausea, dry mouth, and decreased appetite, and are known to have an excellent effect on the degenerative arthritis of the elderly, particularly for headaches of unknown cause. In addition, gastroenteritis, gastritis, gastric ulcers and diarrhea are known to have effects.

Hovenia dulcis THUNB is a deciduous tree belonging to the Rhamnaceae family. It grows in the area below 400m in Chungcheongbuk-do and Gyeongsangbuk-do and is distributed in Japan and China. Fruits, leaves, and branches of the larvae contain beneficial ingredients in the human body such as glucose, malic acid, calcium, furangurinine, hobenin, hobenoside, and habenic acid, which are known to be good for alcohol detoxification and fatigue recovery. It is known to use leaves, stems, and roots as medicinal herbs (Bo Bo-seop and Shin Mingyo, Hyangjedae, Yeonglimsa, pp 291-292, 1998).

Pueraria lobata (WILLD.) And OHWI belong to Leguminosae , and are used to dry the sun's roots of the perennial gland. Brown root contains isoflavone puerarin, puerarin xyloside, daidzein, beta-sitosterol and arackin acid. have. Its main effect is flavones extracted from the roots, which increases blood flow to the brain and coronary arteries, has an effect of palliative and antipyretic effects, and also relaxes muscle spasms (Jung Bo-seop and Shin Min-kyo, Yakdae, Yeonglimsa) , pp 704-705, 1998).

Alnus japonica STEUD. Belongs to the Betulaceae family and is also known as Yuri-mok in Chinese characters. The young leaves and bark of alder trees are called red lambs. Spring and autumn are collected and dried in the sun. Its main ingredients include large amounts of triterpenoids such as lupenone, beta-amylin, glutenol, taraxerol, and betulinic acid. Β-sitosterol, fatty alcohols, pyrocatechol-based tannins are contained. Its effects are known to soothe burns and traumatic hemorrhages, and are effective in purifying and strengthening (Jeong Bo-seop and Shin Mingyo, Hyangjeomsa, Younglimsa, p802, 1998).

In addition, as an herbal preparation, hangover and liver function are improved by using extracts obtained by differently blending persimmon peel, buttercup, ginjin, Schisandra chinensis, mung bean, sauerkraut, chestnut and licorice into one or two or more ingredients. The unique combination of foods "Hangover and liver function improving agent and its manufacturing method by the combination food (Korean Patent Publication No. 2002-81995)", "Low alcohol insoluble having a hangover toxicity and hangover removal activity isolated from the hawthorn tree Extraction fractions and polysaccharide materials and compositions containing them (Korean Patent Publication No. 2002-64151), "Hovenodulinol, an active ingredient of the earth fruit, a method for preparing the same and an alcohol decomposer or hangover containing the same ( Korean Patent Nos. 2002-86963), as well as preparations derived from various plants such as rice roe extract (Gurume), alder, oak vinegar, brownish, and milk thistle extract are effective for hangover. Although it has been reported that, among the various changes caused by drinking, it appears to be effective or ineffective only in some items, and it is difficult to find a big characteristic technique in that it simply uses an extract that has been used as a conventional herbal medicine. In general, the aim is to simply use nutrient tonic or known efficacy. Therefore, there is an urgent need for the development of new oral hangovers and hepatoprotective ingredients without any side effects.

Accordingly, the present inventors have completed the present invention by confirming that the composition of the present invention exhibits a wide range of effects on the hangover caused by drinking, and the liver protection and alcohol metabolism effects are superior to the existing hangover-resolved drinks. .

Therefore, the object of the present invention is not only the excellent side effects of alcohol decomposition and liver protection without side effects, but also has a hangover relieving effect containing the complex herbal extracts of palm cactus, alder, hawthorn and black root easy to take It is to provide a food composition and a health functional food containing the same as an active ingredient.

In order to achieve the above object, the present invention provides a functional food composition for hangover and soy sauce protection containing a complex herbal extract consisting of palm cactus, alder, barberry and brown root.

The composition is palm cactus, alder, bark, barley, 8-12% by weight, palm tree cactus 16-24%, algae 4-8% by weight, barberry fruit 6-10% by weight, barb It is desirable to have a composition ratio of 4-8% by weight of tree trunks.

The extract means an extract available in water and a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, preferably an extract available in water or ethanol.

In addition, the present invention is a health and health care comprising a composition containing a herbal extract of palm cactus, alder, larvae and reeds as an active ingredient and a food supplement acceptable food supplement additives exhibiting a hangover relief and liver protection prevention and improvement effect Provide nutraceuticals.

The dietary supplement includes a form that is a powder, granules, tablets, capsules or beverages, and preferably includes a form of a beverage.

Hereinafter, the present invention will be described in detail.

The extract mixture of palm cactus, alder, hawthorn, and root of the present invention can be obtained by the following method, respectively. Specifically, after collecting the palm cactus, alder, hawthorn, and reeds, and drying and powdered with a crusher, about 2 to 40 times the weight of each dry sample weight of the palm cactus, alder, hawthorn, reeds, preferably Preferably it is about 3 to 10 times the volume of water and C ₁ to C ₄ lower alcohols such as methanol, ethanol, butanol and the like or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably Extraction methods such as soaking, hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for about 30 minutes to 24 hours, preferably 1 to 3 hours at 40 to 100 ° C., preferably 50 ° C., with water or 70% ethanol. Extraction was carried out, and then the above process of recovering the supernatant by vacuum filtration was repeated 2 to 5 times, preferably 3 times, and the supernatant was collected and concentrated under reduced pressure for 24 to 72 hours, preferably 48 hours. During the evaporation or lyophilization, the extracts of palm cactus, alder, cedar, and root roots, which are soluble in water, lower alcohols or mixed solvents thereof, can be obtained.

In this case, the ethanol used as the extraction solvent may be 100% ethanol or 90 to 50% ethanol, and the extraction solvent is not limited to the solvents described above, and all known solvents may be used.

The mixture of the present invention is a mixture of palm cactus, alder, cedar fruit and stem, and root extract obtained by the above method, preferably 8-12% by weight of palm cactus, 16-24% by weight of alder, It can be prepared by mixing with 4-8% by weight of brown root, 6-10% by weight of barberry, 4-8% by weight of bark.

The extract obtained by the above production method can be used in the food composition of the present invention, the extract can be used in the present food composition in the form of a high concentration or powder form of 20 to 80%.

The present invention is a health functional food comprising a composition containing a complex herbal extracts of palm cactus, alder, bark and reed as an active ingredient, and a food supplement acceptable food supplement, which exhibits the effect of preventing hangover and preventing liver improvement. To provide.

Specifically, the functional food composition of the present invention can be used as a health functional food, health beverage or beverage additives, the functional food composition is mixed with 0.001 to 80% by weight based on the total weight of food for functional hangover and liver protection It is preferable to use beverages, pills, tablets or the above ingredients in the form of dry powder filled soft or hard capsules. In this case, when the content of the mixture is less than 0.001% by weight, a large amount of administration may be necessary for effective efficacy, and when the amount exceeds 80% by weight, the efficacy may be similar to a low dose, although it may be uneconomical. Most preferably, the content is appropriately adjusted according to the purpose of the hangover drink.

Examples of the food to which the composite herbal extract of the present invention may be added include various foods, candy, chocolate, beverages, gums, teas, vitamin complexes, health supplements, and the like, powders, granules, tablets, pills, It can be used in the form of a capsule or a beverage.

The health beverage composition of the present invention has no special limitation except for containing the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose, for example polysaccharides such as maltose and sucrose, and conventional sugars such as dextrin and cyclodextrin. And sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract mixture of the present invention.

The complex herbal extract of the present invention has little toxicity and side effects, and therefore can be used with confidence even for prolonged administration for prophylactic purposes.

Complex herbal extract of the present invention may be added to food or beverage for the purpose of relieving hangovers and protecting the liver. At this time, the amount of the food composition of the present invention in the food or beverage is generally of the total food weight 20 to 80% by weight, the beverage composition may be added to the food composition of the present invention in a ratio of 0.02 to 30 g, preferably 0.3 to 1 g based on 100 ml.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.

Example 1.Preparation of Palm Cactus, Alder, Raspberry, Root Extract

After washing and drying each of the palm cactus, alder, hut and reeds purchased from Kyungdong Market, the dried palm cactus 1000g, alder 2000g, walnut fruit 800g, hut stem 600g, reed 600 After grinding each finely with a mill, 500 ml of 70% ethanol was added thereto, soaked at 50 ° C. for 2 hours, and then filtered to obtain a single extract of each. Thereafter, each extract was completely removed by ethanol with a vacuum rotary evaporator (Laborota 4001, Heidolph), concentrated, and then completely dried by lyophilizer for 48 hours to remove 50 g of dry powdered palm cactus and alder 10. g, 40 g of barberry fruit, 30 g of bark tree trunk, and 30 g of root roots were obtained, respectively.

Example 2 Preparation of Complex Herbal Extracts of Palm Cactus, Alder, Raven, and Root

 Each of the palm cactus, alder, barberry fruit, barberry stem, and root extract powder obtained in Example 1 were mixed into 5: 10: 4: 3: 3 to prepare an extract mixture, and then the extract mixture 1 A stock solution (100 mg extract mixture / ml water) in which / 10 was dissolved in 10 ml of water was used for the experiment.

Experimental Example 1. The blood alcohol concentration drop effect of the extract mixture

1-1. Breeding of Experimental Animals

As a test animal, 200 male rats of Sprague-Dawley system with a body weight of 200-250 g were distributed from Daehan Biolink. The animals were bred at a temperature of 23 ± 2 ℃ and relative humidity of 55 ± 10% under constant light and dark cycles from 7 am to 7 pm, and food and water were freely ingested.

1-2. Decreased blood alcohol level

In order to experiment on the hangover effect of the palm cactus, alder, barberry and reed complex herbal extract prepared in Example 2, 3 mg / kg of ethanol at 8 pm was used by using an esophageal needle (jonde) 25 Diluted in% (to a concentration similar to soju), the mixture was then administered 30 minutes. The negative control group was given water, and the positive control group used G company's Y product, which has the largest market share in Korea, as a hangover eliminator. Animals were anesthetized with urethane (Sigma, USA) 1, 2, 4, 6, 8 hours after alcohol administration, and the chest cavity was opened and blood was collected from the heart. 20 animals were used for each time slot. The collected blood was precipitated with 1.8 ml of trichloroacetic acid (serum), centrifuged at 600 x g for 10 minutes, and 1 ml of the supernatant was taken. In addition, an assay kit 333-A (Sigma, USA) was added to a certain amount of the supernatant, and the ethanol content was calculated based on the analysis of the ethanol standard solution.

The results are shown in FIG. 1, and in FIG. 1, in the case of the negative control group administered with water, the blood concentration of alcohol was 120 mg / dl after 1 hour of alcohol intake, and these values were the positive control group or the extract of the present invention. The administration was similar. However, from 2 hours after alcohol administration, it was confirmed that the alcohol of the extract has an effect in reducing blood. That is, in the group administered with the negative control group and the positive control group, the alcohol concentration was slightly increased, but it was confirmed that the blood alcohol concentration was reduced by about 10% when the mixture was administered. On the other hand, 4 hours after the alcohol administration, the blood alcohol concentration was decreased by 32.6% in the positive control group and 36.8% in the mixture compared to the negative control group. After 6 hours of alcohol administration, the positive control group was found to have a blood alcohol lowering effect of 38.5% compared to the negative control group, and a 52.0% lowering effect was observed in the mixture. In addition, after 8 hours of alcohol administration, 74.6% of the positive control group and 79.5% of the mixture compared to the negative control group were able to confirm the blood alcohol inhibitory effect.

Experimental Example 2 Lowering Effects of Alcohol Dehydrogenase (ADH) and Aldehyde Dehydrogenase (ALDH) in Liver of Extract Mixture

2-1. Grinding of liver tissue and preparation of experimental samples

In order to test the hangover effect of the composite herbal extract prepared in Example 2, 3 mg / kg ethanol at 25% (to a concentration similar to soju) using an esophageal needle (jonde) at 8 pm Diluted and then the mixture was administered 30 minutes later. The negative control group was given water, and the positive control group used G company's Y product, which has the largest market share in Korea, as a hangover eliminator. Animals were anesthetized with urethane (urethane) 1, 2, 4, 6, 8 hours after alcohol administration, and the abdominal cavity was opened and liver was collected. Animals were used 20 animals at each time period.

The collected liver tissues were homogenized by adding Tris Buffer (250 mM sucrose, 10 mM Tris, pH 7.4) using a Potter-Elvehyem type homogenizer (Glas-Col, USA). At this time, the amount of Tris buffer was adjusted to 10% (w / v) homogenate. The homogenate was centrifuged at 700 x g for 10 minutes. The supernatant produced at this time was centrifuged at 10,000 × g for 15 minutes, and only the supernatant was collected, and the cytoplasm was separated by ultracentrifugation at 105,000 × g for 1 hour, and the cytosolic fraction was-. The mitochondrial fraction was washed in Tris buffer, and the precipitate formed by centrifugation once more was dissolved in an appropriate amount of Tris buffer (mitochondrial fraction) and stored until measuring ADH activity.

2-2. Identification of Alcohol Dehydrogenase in Liver Tissues

Determination of alcohol dehydrogenase (ADH) was performed by mixing 50 mM glycine buffer (pH 9.6), 0.8 mM NAD, 3 mM ethanol, and 50 μl cytosolic fraction in a total of 1 ml of reaction mixture. As a result, the change of ADH with time was confirmed.

The results are shown in FIG. 2, and in FIG. 2, the concentrations of ADH were similar at 1 hour after alcohol administration in both the negative control group, the positive control group, and the group administered the mixture of the present invention. , The concentration of ADH in the group administered with the mixture was found to be maintained about 60% higher than the control group administered with water, the positive control group continued to rise, but about half of the mixture Only ADH synergy was found.

2-3. Identification of Aldehyde Dehydrogenase in Liver Tissue

Aldehyde dehydrogenase (ALDH) is 100 mM pyrophosphate buffer (pH 8.0), 1 mM NAD, 2 mM pyrazole, 15 mM acetaldehyde, 50 μl in a total of 1 ml of reaction mixture. Mitochondrial fraction was added and measured.

The results are shown in FIG. 3, and in FIG. 3, the concentrations of ALDH at 1 hour after alcohol administration were similar in both the negative control group, the positive control group, and the group administered the mixture of the present invention. In the case of administration of the mixture, the concentration of ALDH was maintained at about 50% higher than that of the negative control group administered with water, and in the case of the positive control, it was maintained at 25% higher. It could be confirmed and continuously increased, but the synergistic effect was confirmed to be lower than that of the mixture.

Hereinafter, an example of the preparation of the composition containing the complex herbal extract of the present invention will be described, but the present invention is not intended to limit the present invention but is only specifically intended to be described.

Formulation Example 1 Preparation of Health Functional Food

1000 mg of the composite herbal extract of Example 2

20 g of vitamin mixture

70 μg of Vitamin A Acetate

Vitamin E 1 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food production method. The granules may be prepared and used for preparing the nutraceutical composition according to a conventional method.

Formulation Example 2 Preparation of Health Beverage

ingredient Composition ratio (%) Complex herbal extract of Example 2 50 Vitamin c 6 Vitamin E 6 Taurine 5 Citric acid 5 Ginger juice 5 Bean Sprout Juice 5 licorice 6 cinnamon 5 Ginseng 2 Situation Mushroom 5 Sum 100

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention It is used to prepare a health functional beverage composition.

Formulation Example 3 Preparation of Capsule

ingredient Composition ratio (%) Complex herbal extract of Example 2 95 Vitamin c One Vitamin E 1.5 Taurine 2.5 Sum 100

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

As described above, the composition for releasing hangover of the present invention is an active ingredient of natural herbal medicines such as palm cactus, alder, hawthorn and reeds, so that there is no side effect to the human body, and thus the hangover relieving effect is excellent, and the liver protection effect is excellent. It can be usefully used in the functional food field, such as functional drinks, health functional food for hangover relief.

Claims (5)

delete delete delete The mixing ratio of the fruit of the palm cactus ( Opunita ficus indica ), alder ( Alnus japonica STEUD.), The root ( Pueraria lobata (WILLD.), OHWI), the fruit of the tree ( Hovenia dulcis THUNB.) And the stem of the tree : 16-24: 4-8: 6-10: A dietary supplement for hangover and soy sauce protection, comprising 4-8% by weight of complex herbal extracts and food acceptable food supplements. delete

Images