KR19990046012A - The production method of tea or drink utilizing the myceloid Phellinus Linteus or Agaricus blazei - Google Patents

Abstract

The present invention relates to a method for producing tea and beverage using mushrooms and / or mushroom mushroom mycelium, assaying whether or not contaminated by the fruiting body or tissue of the mushroom or mushroom mushroom 1 to 2 times in Agar medium After shaking culture in a liquid medium, 22 to 30 in a fermenter with a liquid medium consisting of 2 to 5% carbon source, 0.3 to 0.5% nitrogen source, 0.05% MgSO ₄ ㆍ 7H ₂ O and 0.05% KH ₂ PO _4. Incubate for 30 to 70 days at a temperature of 80 ℃ to a stirring rate of 80 to 200ppm to form a mycelium of mushrooms or mushrooms, then the mycelium mass is juiced and heated to boil for about 30 minutes, filtered through a strainer and filtered 1 to 50 times the amount of water, sweeteners, vitamins, acidulants and other natural extracts are mixed and heated and sterilized at a temperature of 90 to 100 ° C. to prepare a beverage, or the filtrate is concentrated to obtain a concentrate, and thus sweeteners, vitamins, Acidic acid and other natural extracts are added to dry or roasted brown rice, ganoderma lucidum, Angelica, jujube, green tea leaf, 칡, chicory, shiitake, ginseng, etc. Tea or beverages using fragrant situational mushrooms and / or spiritual mushroom mycelium could be obtained.

Description

The production method of tea or drink utilizing the myceloid Phellinus Linteus or Agaricus blazei}

The present invention relates to a method for preparing tea and beverages using Phellinus Linteus and / or Agaricus blazei mycelium, and more specifically, to agar (Agar) of fruiting bodies or tissues of P. After subculture in medium, assayed for contamination and shaken in liquid medium, followed by carbon source 2-5%, nitrogen source 0.3-0.5%, MgSO ₄ ㆍ 7H ₂ O 0.05% and KH ₂ PO ₄ 0.05% In a fermentation tank consisting of a liquid medium consisting of a culture medium for 30 to 70 days at a temperature of 22 to 30 ℃ at a stirring speed of 80 to 200ppm to form a mycelium of a situation mushroom or a new mushroom, the mycelium mass is juiced and heated Boil for about 30 minutes, and then strain through a strainer to mix 1 to 50 times the amount of water, sweeteners, vitamins, acidulants and other natural extracts and heat sterilize at a temperature of 90 ~ 100 ℃ to prepare a beverage, or the filtrate of Concentrate to obtain a concentrated liquid and dry it by adding sweeteners, vitamins, acidulants and other natural extracts, or by adding powders such as roasted brown rice, ganoderma lucidum, donkey, jujube, green tea leaves, 칡, chicory, shiitake, ginseng, etc. It relates to a method for producing tea and beverages using the situation mushroom and / or mushroom mushroom mycelium characterized in that.

Phellinus linteus (wood mud mushroom) is a rare mud fungus native to the mulberry tree, which has been reported to have the strongest anti-tumority among mushrooms.

Currently, the treatment of cancer includes chemotherapy, radiation therapy and surgical procedures, but the range of treatment is limited and its use is limited due to the manifestation of side effects. In view of this, there have been attempts to treat harmless and more efficient treatment of humans by replacing the existing chemotherapy treatments with direct cytotoxicity or revitalizing the host's immune activity against cancer cells. Bacteria such as BCG and Zymosan, a cell wall polysaccharide of Saccaromyces cerevisiae, are known to stimulate the host's immunity and exhibit anti-cancer activity. It has been reported that lipopolysaccaride, proteoglycan and hemicellulose isolated from herbal, lichen herbal and various plant herbals have host mediated antitumor activity.

Recently, the anticancer activity of polysaccharides or protein polysaccharides isolated from basidiomycetes, such as Lentinan from shiitake mushrooms, PK and copolang from cloud mushrooms and proteoglycans from white silk mushrooms, has been found. Or is under development.

On the other hand, Ikekawa et al. Reported that the situation had the strongest host-mediated anti-tumor effect in the comparative studies of anti-tumor polysaccharides of basidiomycetes, but the situation is a parasitic mushroom in the mulberry tree. It is very rare to grow naturally in nature, and with the reduction of mulberry, it is difficult to collect the fruiting body, so there is no resource, and the artificial culture is also extremely difficult, and it is not practically used to this day.

A situation in culture and extraction of the mycelium by the method of harvesting the fruit body situation by sanmyeong culture separate the mycelium from the agar medium and then cultured, sawdust 1,000g, soy flour 250g, peptone 3.0g, 5.0g KNO _3, CaCO ₃ 7.5g Inoculate the mycelia into a solid medium consisting of 1.8 to 2.0 l of distilled water, incubate at 25 ° C. for 3 months in an incubator, and reflux 100 g of dried mycelium with 1,000 ml of hot water for 1 hour and extract the extract at 100 ° C. The mixture is concentrated under reduced pressure to 100 ml at the following low temperature, and then the concentrate is lyophilized to obtain a powder.

However, the conventional situation mycelium cultivation method is a method for using the mycelium for medical use, the method of cultivating and extracting the mycelium is difficult, there is a problem that the cost of cultivating and extracting the mycelium is expensive.

Meanwhile, Agaricus blazei belongs to the genus of fold mushrooms and fold mushrooms in bioscenarios, and the results of the component analysis were revealed by Dr. WJ Sinden of Pennsylvania State University and Dr. ED Lambert of Lambert Institute (Pennsylvania State University Journal). No. 482 (1965)), which contains a large amount of vitamins, minerals, nucleic acids, amino acids, and enzymes.In 1978, the Iwade Mycology Research Institute in Japan used woolen soils on compost such as rice straw and sugar cane. The "cultivation method" of house cultivation, which inoculates spawning seeds at the source, has been established, and Tokyo University Pharmacy and National Cancer Center have published research findings that the new mushrooms have strong anticancer effects.

In addition, since the experimental results of the anticancer effect of the new mushrooms were published at the 39th Japan Cancer Society in November 1980, numerous studies have been conducted. Betaglucan, alphaglucan, galactoglucan, Polysaccharides such as xyloglucan and protein glucan have been identified, and studies have been made on steroids, nucleic acid components, lipid components, lectins, etc., which have anticancer effects, and their structures and activities have been found.

In addition, when compared with other mushrooms and components, rich in protein content, rich in various amino acids, vitamins and minerals higher than the average, and contains a lot of linoleic acid that acts to lower blood cholesterol of high blood pressure caused by arteriosclerosis.

In addition, in the case of the cultivation of the situation mushrooms or young mushrooms, since most of the solid medium is used, the medium material is not only expensive but also the infection of bacteria or viruses in the production of the liquid spawn cannot be confirmed. Not only has the disadvantage of having to discard all of the culture medium, it takes about 50 to 65 days to form mycelium, there is a problem that the yield is not much.

On the other hand, when manufacturing foods such as tea using a situation mushroom or a ghost mushroom, the medium itself uses a lot of grains, but in order to use grains as a medium component, sterilize the grains in water and sterilize them at high temperature and high pressure. Since the sugar is discharged from the grains and the mycelium of the situation mushrooms or spiritual mushrooms is cultivated, the nutrients of the grains are depleted, so it is difficult to produce satisfactory aroma and taste when preparing foods such as tea.

Therefore, the object of the present invention is not only economical low-cost mushroom or medium mushroom culture medium material to obtain a large amount of situation mushroom or fresh mushroom mycelium, it is economical and convenient and does not require a high level of technology easily Tea obtained by using the situation mushroom and / or Spirit mushroom mycelium having a taste and aroma that satisfies the general consumer by obtaining the situation mushroom or spiritual mushroom mycelium, and manufacturing tea and beverage using the cultured mushroom or mushroom mushroom mycelium. It's about serving drinks.

Another object of the present invention is to provide a method for producing a tea and a beverage of the above object.

In order to achieve the above object as well as another object that can be easily expressed in the present invention, the fruit body or tissue of the situation mushroom or a new mushroom in agar (Agar) passage 1 to 2 times to test whether the contamination after liquid After shaking culture in the medium, the temperature of 22-30 ° C. in a fermenter with a liquid medium consisting of 2-5% carbon source, 0.3-0.5% nitrogen source, 0.05% MgSO ₄ 7H ₂ O and 0.05% KH ₂ PO _4. After incubation for 30 to 70 days at 80-200ppm stirring speed to form mycelium of situational mushrooms or young mushrooms, the mycelium mass was juiced and heated and boiled for about 30 minutes, filtered through a strainer, and filtered into 1-50. A batch of water, sweeteners, vitamins, acidulants and other natural product extracts are mixed and heated and sterilized at a temperature of 90 to 100 ° C. to prepare a beverage, or the filtrate is concentrated to obtain a concentrate and sweetener, vita Dried by adding acid, acid and other natural extracts, or roasted brown rice, ganoderma lucidum, Angelica, jujube, green tea leaf, 칡, chicory, shiitake, ginseng and other powders to make tea satisfactory to consumers Tea or beverage using the situation mushrooms and / or spiritual mushroom mycelium with the fragrance could be obtained.

The present invention is described in more detail as follows.

Tea and beverage production method using the situation mushroom and / or mushroom mushroom mycelium according to the present invention by passing the fruiting body or tissue of the situation mushroom or spirit mushroom in the agar (Agar) 1 to 2 times after assaying for contamination After shaking culture in a liquid medium, the mixture was added with a liquid medium consisting of 2 to 5% carbon source, 0.3 to 0.5% nitrogen source, 0.05% MgSO ₄ · 7H ₂ O and 0.05% KH ₂ PO ₄ to be added at 22-30 ° C. Incubate for 30 to 70 days at a stirring speed of 80 to 200 ppm at a temperature to form a mycelium of a situation mushroom or a new mushroom. Then, the mycelium mass is juiced and heated, boiled for about 30 minutes, filtered through a strainer, and then filtered into a filtrate. 50 times of water, sweeteners, vitamins, acidulants and other natural product extracts are mixed and heated and sterilized at a temperature of 90 to 100 ° C. to prepare a beverage, or the filtrate is concentrated to obtain a concentrated liquid and thus sweeteners, vitamins, acidulants Other natural extracts added to dried, or by the addition of this roasted brown rice, Ganoderma lucidum, angelica root, date palm, powder of green tea leaves, arrowroot, chicory, elevation, ginseng characterized by producing a difference.

First, the fruiting bodies or tissues of the situation mushrooms or spiritual mushrooms are passaged once or twice in Agar medium to test whether they are contaminated, and then 2 to 5% carbon source, 0.3 to 0.5% nitrogen source, and MgSO ₄ ㆍ 7H ₂ O 0.05 After aseptically inoculated into a liquid medium consisting of 0.05%% and KH ₂ PO ₄ and the remaining amount of distilled water, the mixture is shaken in a shaker at 80 to 200 rpm and 22 to 30 ° C.

Then, at a temperature of 22 to 30 ° C. in a fermentation tank to which a liquid medium composed of 2 to 5% carbon source, 0.3 to 0.5% nitrogen source, 0.05% MgSO ₄ · 7H ₂ O and 0.05% KH ₂ PO ₄ was added. Incubate for 30 to 70 days at a stirring speed of 200 ppm.

As the carbon source used in the culture medium, at least one selected from the group consisting of sugar, maltose, fructose, glucose, sucrose, malt extract and syrup, and at least one selected from soy flour or peptone as a nitrogen source Can be used.

The culture method as described above is a method commonly used in the art to which the present invention belongs, and the culture medium composition is also a medium composition commonly used in the art to which the present invention belongs.

By culturing as described above, it is possible to replace the culture medium components with cheap ones, so it is very economically advantageous to obtain mycelium of situation mushrooms or young mushrooms, and the culture medium is contaminated with various bacteria, but it is not confirmed. Even if it is contaminated, it is possible to solve the conventional problem of disposing all of the culture medium by disposing only the medium identified in the culture, and remarkably than the conventional method using the solid medium even if all the culture medium is disposed of. Economics can be obtained.

In addition, compared to the case of using a conventional solid medium was a short period of time to obtain a mycelium of the situation mushrooms or spiritual mushrooms was effective.

After completion of the main culture in the same manner as above to produce mycelium of the situation mushroom or aging mushroom, the mycelium mass is juiced and heated, boiled for about 30 minutes, filtered through a strainer and filtered 1-50 times the amount of water, sweetener , Vitamins, acidulants and other natural extracts are mixed and heated to sterilize at a temperature of 90 ~ 100 ℃ to prepare a beverage, or the filtrate is concentrated to obtain a concentrate, and added sweeteners, vitamins, acidulants and other natural extracts and dried or Prepared tea is added to the roasted brown rice, ganoderma lucidum, Angelica, jujube, green tea leaves, 칡, chicory, shiitake, ginseng and the like.

That is, by extracting the cultured mushrooms or mushroom mushroom mycelium to remove the medium component to be contained in the liquid culture, heated and boiled for about 30 minutes while extracting the nutrients or pharmacological components contained in the mushrooms or mushroom mushrooms, the solid content Remove the situation mushrooms or mushrooms mycelium mycelium extract to obtain.

Then, 1 to 50 times of water, sweeteners, vitamins, acidulants and other natural product extracts are mixed and heated and sterilized at a temperature of 90 to 100 ° C. to prepare a beverage.

Alternatively, concentrate or extract of mushroom or mycelium mycelium mycelium extract to concentrate and add sweeteners, vitamins, acidulants and other natural extracts to dried or dried concentrate and roasted brown rice, ganoderma lucidum, angelica, jujube, green tea leaf, 칡, chicory Tea is prepared by mixing and powdering powders such as shiitake and ginseng.

In particular, in the present invention, at least one selected from the group consisting of brown rice powder, ganoderma lucidum powder, Angelica powder, jujube powder, green tea leaf powder, 칡 powder, chicory powder, yulmu powder, roasted in the mycelium powder of the situation mushroom or spiritual mushroom The above powders are mixed and packaged to produce edible or medicinal teas.

In the present invention, by using the roasted brown rice in particular, using the same grain as the conventional ingredients such as brown rice as a medium component was able to solve the problem of the taste and improvement of the grain produced in the tea using the same as the mycelium of the situation mushroom or spiritual mushroom .

In addition to the roasted brown rice powder, ganoderma lucidum powder, Angelica powder, jujube powder, green tea leaf powder, 칡 powder, chicory powder, yulmu powder can be used in the technical field to which the material can be commonly used in the art It can be easily inferred.

At least one powder selected from the group consisting of mycelium powder, roasted brown rice powder, roasted brown rice powder, ganoderma lucidum powder, jujube powder, jujube powder, green tea leaf powder, 칡 powder, chicory powder, and yul mushroom powder The ratio of 1: 2 to 2: 1 was effective in terms of the taste and aroma of the tea produced.

The following examples illustrate the invention in more detail, but do not limit the scope of the invention.

Example 1

The fruiting bodies or tissues of the situational mushrooms and new mushrooms were each passaged twice in Agar medium to test whether they were contaminated and shaken in liquid medium, followed by 5% carbon source, 0.5% nitrogen source and MgSO ₄ ㆍ 7H ₂ O. The mycelium of the situation mushroom and the aging mushroom was formed by culturing for 40 days at a temperature of 26 ° C. and a stirring speed of 140 ppm in a fermentation tank containing 0.05% and 0.05% KH ₂ PO ₄ .

On the other hand, while producing a mycelium of mushrooms or mushrooms by the method of using a known solid medium, compared to the method of producing a mycelium of the mushrooms or mushrooms of the above state, the degree of production and mycelial density of the mycelium was visually confirmed. It was confirmed that more than the mycelium produced by the method using a solid medium, the method of the present invention was much more effective considering the mycelium production period of the situation mushroom or young mushroom.

In addition, by producing a mycelium of mushrooms or mushrooms mushrooms in the same manner as described above, the cultured mushrooms or mushrooms mushroom mycelium cultured, heated and boiled for about 30 minutes while boiling or nutrients contained in the mushrooms or spirit mushrooms After extracting the pharmacological components, the solids are removed to obtain a mycelium mushroom or a mycelium mushroom mycelium extract.

Then, concentrated mushroom or myrtle mushroom mycelium extract concentrated, dried 200g and roasted brown rice, ganoderma lucidum, Angelica, jujube, green tea leaves, 칡, chicory, shiitake, ginseng 25g each to make tea to 100 panelists As a result of drinking, satisfactory results were obtained in taste (average grade: 7.98) and flavor (average grade: 7.74).

※ Sensory Evaluation Criteria

● Very good. : 9 points

● Outstanding : 7 points

● Normal. : 5 points

● Bad. : 3 points

● Very bad. : 1 point

Example 2

10 g of known sweeteners, 20 g of vitamin C, 10 g of acidulants, and 10 g of ganoderma lucidum, jujube, shiitake and ginseng extract were added to 10 g of situation mushrooms or mushroom fungus mycelium extracts, and tea was dried in the same manner as in Example 1. As a result of manufacturing and evaluating the taste and the aroma in the same manner as in Example 1, it was possible to confirm the excellent quality of the average score of 8.07 in taste and the average score of 7.97 in aroma.

Example 3.

To 10 g of situation mushroom or mushroom fungus mycelium extract, add 200 g of water, 10 g of known sweetener, 10 g of vitamin C, 10 g of acidulant, and 10 g of ganoderma lucidum, Angelica, jujube, shiitake, ginseng extract, and heat sterilization for 1 minute at 95 ℃. Except that was prepared in the same manner as in Example 1, and the taste and aroma were evaluated in the same manner as in Example 1, it can be confirmed that the excellent quality of the average rating 8.01 in taste, the average rating 7.82 in aroma. there was.

As described above, in the present invention, the fruiting bodies or tissues of the situation mushrooms or spiritual mushrooms are passaged 1-2 times in Agar medium to test whether they are contaminated and then shaken in a liquid medium, followed by carbon source 2-5%, nitrogen source. Incubate for 30 to 70 days at a temperature of 22 to 30 ° C at a stirring speed of 80 to 200 ppm in a fermentation tank containing a liquid medium consisting of 0.3 to 0.5%, MgSO ₄ .7H ₂ O 0.05% and KH ₂ PO ₄ 0.05%. After forming the mycelium of the situation mushroom or spiritual mushroom, the mycelium mass is extracted, heated and boiled for about 30 minutes, filtered through a strainer and filtered in a filtrate with 1 to 50 times the amount of water, sweeteners, vitamins, acidulants and other natural substances. The extract is mixed and heated and sterilized at a temperature of 90 to 100 ° C. to prepare a beverage, or the filtrate is concentrated to obtain a concentrate, which is then added with sweeteners, vitamins, acidulants and other natural extracts, or dried. Prepared by adding powders such as roasted brown rice, ganoderma lucidum, angelica, jujube, green tea leaf, 칡, chicory, shiitake, ginseng, etc., to prepare situational mushrooms and / or spiritual mushroom mycelium that have a taste and aroma satisfying to consumers. Could get used tea or drink.

Claims (3)

Fruiting bodies or tissues of situational mushrooms or young mushrooms are passaged once or twice in Agar medium and tested for contamination, and then shake-cultured in liquid medium, followed by carbon source 2-5%, nitrogen source 0.3-0.5%, MgSO ₄ · 7H ₂ O 0.05% and KH ₂ PO ₄ 0.05% of the situation in the fermentation tank with a liquid medium added at a temperature of 22 ~ 30 ℃, 80 to 200ppm stirring rate for 30 to 70 days After forming mycelium of young mushrooms, the mycelium mass is extracted, heated and boiled for about 30 minutes, filtered through a strainer, and then mixed with filtrate with 1 to 50 times water, sweeteners, vitamins, acidulants and other natural extracts. Method for producing a beverage using a mushroom or mycelium mushroom mycelium, characterized in that the heat sterilization at a temperature of 100 ℃. Fruiting bodies or tissues of situational mushrooms or young mushrooms are passaged once or twice in Agar medium and tested for contamination, and then shake-cultured in liquid medium, followed by carbon source 2-5%, nitrogen source 0.3-0.5%, MgSO ₄ · 7H ₂ O 0.05% and KH ₂ PO ₄ 0.05% of the situation in the fermentation tank with a liquid medium added at a temperature of 22 ~ 30 ℃, 80 to 200ppm stirring rate for 30 to 70 days After forming mycelium of young mushrooms, the mycelium mass is extracted, heated and boiled for about 30 minutes, filtered through a strainer to obtain a filtrate, and concentrated by adding sweeteners, vitamins, acidulants and other natural extracts. Method of producing a tea using the mushroom or mycelium mushroom mycelium characterized in that. The method of claim 2, wherein the filtrate is concentrated and dried, and at least one selected from the group consisting of brown rice, ganoderma lucidum, Angelica, jujube, green tea leaf, sauerkraut, chicory, shiitake, and ginseng powder is added thereto. Method for producing tea using the situation mushroom or mushroom mycelium.