KR20010077679A - Additive composition for animal feed - Google Patents

Abstract

PURPOSE: Provided is a composition of feed additive containing chitin, chitosan and chitosan oligosaccharide as active principles, which can promote growth of livestock and decrease cholesterol of the livestock. CONSTITUTION: The composition includes 15-25% of crude protein, 5-15% of crude fat, 7-14% of crude fiber, 17-30% of crude ash, 0-18% of moisture, 2-5% of calcium, 1-3% of phosphorus, 0-4% of mannan, 0-4% of glucan, 0-30,000unit/kg of amylase, 0.00001-0.0001% of carotenoids antioxidant, 2.0-20% of chitin and chitosan and 2.0-20% of chitosan oligosaccharide.

Description

Additive composition for animal feed

The present invention relates to a composition for animal feed additives. More specifically, the present invention relates to a feed additive composition comprising chitin, chitosan and chitosan oligosaccharides as active ingredients.

Chitin was first discovered in 1811 when French historian Fracono discovered an unknown immune component contained in mushrooms and called it 'breakout'. Later, in 1823, the scientist of French quality, called it Chitin, the Greek envelope in the sense of a substance that forms the skin of a mushroom. In 1859, Gera was discovered by a scientist, Chitosan, a hydrolyzed form of chitin.In 1982, Japan's Ministry of Agriculture, Forestry and Fisheries reported 10 years after the death rate from adult diseases such as cancer, hypertension, diabetes and heart disease increased. The research on biomaterials, Bio-Mass, which was carried out by the plan, proceeds with the development of chitin and chitosan, which prevents the growth of bacteria, viruses, cancer cells, etc. and strengthens immune cells. It has come to practical use. In the 1990s, it began to be used for health food, agriculture, and industrial purposes. In 1996, it was used for the first time in the livestock industry.

Chitin is a natural animal fiber that exists in the natural world that constitutes the shell of lower organisms. More than 100 billion tons are produced annually, and the molecular weight of N-Acetyl Glucosamine is bound to β1-4. More than one million high molecular materials. Chitosan, on the other hand, is a polymer obtained from chitin through a deacetylation reaction, and the macromolecule of chitin is a low molecular form. The chitosan made in this way is insoluble in water and alcohol solvents, has a characteristic of being soluble in weak acid, and is a pollution-free and non-toxic biodegradable substance. On the other hand, the low molecular weight of chitin with molecular weight of 100,000 to 200,000 once again to break down 2 to 10 glucosamine is called chito-oligosaccharide, which is the best form of body absorption compared to chitin and chitosan. to be. The structural formulas of chitin and chitosan are as shown in the following general formula (I) and the properties of chitin, chitosan and chitosan oligosaccharides are shown in Table 1 below.

Characteristics of major chitin derivatives characteristic Chitin Chitosan Chitosan oligosaccharides Solubility Poorly soluble Soluble receptivity Biodigestion Indigestible Partially digested Full digestion Palatability - A bitter taste Sweetness Functional - Multifunctional Multifunctional

As shown in Table 2, chitin chitosan and chitosan oligosaccharides are divided into three types: chemical hydrolysis using hydrochloric acid and caustic soda, enzymatic digestion using chitin-chitosanase enzyme, and microbial fermentation using microorganism and fermentation medium. In the case of the microbial fermentation method, it is difficult to proceed with work and requires a lot of know-how, but it is the most stable and high functional method.

Production of chitin chitosan and chitosan oligosaccharides Manufacturing method Chemical hydrolysis Enzyme digestion Microbial fermentation Decomposition Hydrochloric acid and caustic soda Chitin-chitosanase Microorganisms and Fermentation Medium Manufacturing cost lowness height lowness Work in progress ease difficulty difficulty safety Residual chemicals such as hydrochloric acid height height Functional lowness height Very high Pollutant Emissions height middle none Palatability Falling Medium good

Chitin, chitosan and chitosan oligosaccharides are applicable to a wide variety of fields due to the various physiological activities of these substances and are actually used in many fields. Chitin is applied to wound healing, artificial skin (suture), textiles, fertilizers, industrial speaker membranes, and chitosan is mainly applied to fermentation accelerators, cellular activators, cholesterol lowering agents, hygroscopic agents, immunostimulants, hair products, cosmetics and wastewater treatment flocculants. Oligosaccharides have been applied as cell activators, immunoactivators and natural activators.

The inventors of the present invention, chitin chitosan and chitosan oligosaccharides, crude protein (crude fat), crude fat (crude fat), crude ash (crude ash), moisture (calcium), phosphorus (phosphorus), A composition containing a certain amount of antioxidants of mannan, glucan, amylase and carotenoid-based antioxidants is used to improve feed efficiency with animal feed additives such as weaning pigs, piglets, laying hens, lactating cows and finishing pigs. In addition, the present invention has completed the present invention by confirming the effect of increasing the intestinal useful microorganisms in large quantities and reducing the death of typhoid as well as lowering cholesterol.

Accordingly, an object of the present invention is to provide a composition for animal feed additives containing chitin chitosan and chitosan oligosaccharide as an active ingredient.

The object of the present invention is chitin chitosan and chitosan oligosaccharides and crude protein (crude protein), crude fat (crude fat), crude fiber (crude fiber), crude ash (moisture), calcium (calcium), phosphorus ( phosphorus, mannan, glucan, amylase and amylose, and a carotenoid antioxidant, each of which was formulated to prepare a composition for animal feed additives and prepared the composition for weaning pig feed, pig feed or The present invention was completed by adding to a laying hen's feed and feeding the livestock, and investigating feed efficiency, antibiotic effect, enteric beneficial bacterium growth effect, typhoid mortality reduction effect, cholesterol lowering effect and the like.

Hereinafter, the configuration of the present invention will be described.

Figure 1 is a graph showing the reduction of mortality due to the feed and feed composition for livestock feed additives of the present invention in laying hens.

Figure 2 is a photograph showing the antimicrobial effect of the composition for animal feed additives of the present invention.

The present invention is chitin chitosan and chitosan oligosaccharides and crude protein (crude protein), crude fat (crude fat), crude fiber (crude fiber), crude ash (moisture), moisture (calcium), phosphorus (phosphorus), Preparing a composition for animal feed additives by combining mannan, glucan, amylase and amylose and a carotenoid antioxidant each by a predetermined amount; The step of investigating the effectiveness and replacement of antibiotics as a weaning pig feed additive by comparing the weight gain and the control group fed the weaning feed containing the composition of the present invention for animal feed additives prepared in the weaning pig and then the antibiotic feed containing antibiotics ; Investigating the efficiency and the substitution of antibiotics as pig feed additives by comparing the weight gain with the control group fed the feed containing the composition for animal feed additives to piglets and the feed containing antibiotics; The composition of the present invention feed additive composition according to the present invention by comparing the egg production rate, egg shell quality, king special egg production rate, egg weight, eggshell degree before and after feeding the feed containing the composition for animal feed additives to the laying hens Investigating the effectiveness as a feed additive; The feed containing the composition for animal feed additives of the present invention was fed to lactating cows, and the control group fed the feed containing antibiotics, milk yield, milk fat content and somatic cell count, respectively, and containing the composition for animal feed additives of the present invention. Examining the somatic cell count before and after feeding the feed to the dairy cow to investigate the efficiency of the composition for animal feed additives of the present invention as the non-dairy feed additive; The present invention was prepared by comparing the feed containing the composition for feed additives of the present invention to the finishing pigs and feeding the antibiotic-containing feed or the control diet and the back fat thickness of the control group fed the basic diet. Investigating the effectiveness as a hog feed additive; Feeding the feed containing the composition for animal feed additives of the present invention to mice and counting Lactobacillus acidphillus to investigate the effect of the composition for animal feed additives on the growth of enteric beneficial bacteria by counting Lactobacillus acidphillus ; Investigating the effect of the composition for the feed composition of the present invention on mortality caused by typhoid by comparing the feed containing the composition for animal feed additives to the laying hen before and after feeding the egg laying mortality due to typus respectively ; The livestock of the present invention by feeding the feed containing the composition for animal feed additives of the present invention and the feed not containing the composition for animal feed additives of the present invention to cows respectively and comparing and measuring the contents of HDL-cholesterol and LDL-cholesterol in the blood, respectively. Examining the effect of the composition for feed additives on lowering cholesterol in livestock blood; Investigating the antimicrobial activity of the composition for animal feed additives of the present invention against E. coli 0157, Salmonella typimurium by the paper disk method.

The composition for animal feed additives of the present invention functions to inhibit the activity of pathogenic microorganisms by reacting cationic amine groups of oligosaccharides such as chitin, chitosan, chitosan oligosaccharides, mannans and glucans with anionic carboxyl groups of microbial cell walls. In addition, the antimicrobial activity is higher in the low molecular weight chitosan than the chitosan of the polymer, chitosan oligosaccharides have a high antimicrobial activity in the six-mer or more. In particular, the composition for animal feed additives of the present invention shows a strong antimicrobial effect against Salmonella, showing an excellent effect in preventing infection of Salmonella.

The composition for animal feed additives of the present invention has the effect of enhancing the body's immunity by activating T-cells, macrophages and natural killer cells in the immune system.

The carotenoid antioxidants contained in the composition for animal feed additives of the present invention may use β-carotene or astaxanthin, and these may act as bioprotective agents together with SOD and α-tocopherol (vitamin E). It has the effect of strongly inhibiting the formation of lipid peroxide, which causes free-radical chain reaction, and improves egg yolk color as well as egg shell quality through natural coloring effect. There is. Furthermore, when the natural animal astaxanthin is included in the composition for animal feed additives of the present invention, it may exhibit an antioxidant effect 10 times or more compared with the conventional synthetic astaxanthin.

Oligosaccharides such as chitosan oligosaccharides, mannans and glucans contained in the composition for animal feed additives of the present invention serve to promote the proliferation of useful microorganisms with various immune enhancing effects, and oligosaccharides are generally referred to as bifidus factor. The reason is that only beneficial bacteria are available sugars.

Various fermented metabolites and useful microorganisms contained in the composition for animal feed additives of the present invention have an intestinal stabilizing effect by inhibiting the growth of pathogenic microorganisms such as Escherichia coli and Salmonella, and promoting the growth of useful microorganisms, and in addition, feed efficiency And improving palatability to improve the daily weight gain of livestock. Especially useful microorganisms such as lactic acid bacteria, yeast, Aspergillus oryzae , Aspergillus niger , Bacillus subtilis and Streptomyces spp. Containing up to 4 billion to 400 billion each per kg composition for the animal feed additives of the present invention has the effect as shown in Table 3 through the various functions they have.

Effect of microorganisms contained in the composition for animal feed additives of the present invention microbe effect Lactic acid bacteria Produces lactic acid, lowers pH, produces natural antibiotics, produces homegrown vitamins, amino acids, nucleic acids, and antibacterial substances. Saccharomyces cerevisiae As active yeast, it increases digestion by acting yeast secretory enzyme in livestock digestive tract, promotes degradation of hardly degradable nutrients such as cellulose, and enhances palatability by action of alcohol and glutamic acid Aspergillus duck , Niger (Aspergillus oryzae, niger) It has excellent starch saccharification ability and protein degrading ability, improves utilization rate, produces various organic acids, promotes gastric acid saving and promotes enzyme activation, and is present in powder to improve the value of fermented fertilizer and organic fertilizer. Bacillus subtilis Release of in vitro secreting enzymes such as amylase and proteinase, produce DPA (dipicolinic acid) antibacterial material to inhibit harmful microorganisms, promote lactic acid bacteria growth, and synthesize vitamin B group. Streptomyces spp. Antibiotic production, chitinase enzyme action, chitin breakdown, jasmine secretion, and other microbial inhibition antagonism.

The chitosan and chitosan oligosaccharides contained in the composition for animal feed additives inhibit the action of lipolytic enzymes, promote the release of bile acids and lower cholesterol and triglycerides, thereby inhibiting the deposition of fat and cholesterol in the blood or liver. It works. That is, it lowers LDL cholesterol in the blood and liver and relatively raises HDL cholesterol. In particular, the ionic interaction and hydrophobic nature of chitosan have the strongest reaction with bile acids, especially cholic acid.

Preferred composition for animal feed additives in the present invention is crude protein (crude protein) 15-25%, crude fat (crude fat) 5-15%, crude fiber (crude fiber) 7-14%, crude ash (crude ash) 17-30% , 0 to 18% moisture, 2 to 5% calcium, 1 to 3% phosphorus, 0 to 4% mannan, 0 to 4% glucan, amylase 0 to 30,000 units / kg, 0.00001 to 0.0001% of carotenoid antioxidants, 2.0 to 20% of chitin and chitosan, and 2.0 to 20% of chitosan oligosaccharides.

Hereinafter, specific embodiments of the present invention will be described in detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited only to these Examples.

Example 1: The composition for animal feed additives of the present invention

The composition for animal feed additives of the present invention is a natural bioactive substance made by incubating chitosan-producing bacteria, chitin and chitosan oligosaccharide-producing bacteria, and lactic acid bacteria with fermentation aids in grain and whey medium, crude protein (crude protein) 15 to 25%, crude fat (crude) fat 5-15%, crude fiber 7-14%, crude ash 17-30%, moisture 0-18%, calcium 2-5%, phosphorus 1 to 3%, mannan 0 to 4%, glucan 0 to 4%, amylase 0 to 30,000 unit / kg, carotenoid antioxidant 0.00001 to 0.0001%, chitin and chitosan 2.0 to 20% , Chitosan oligosaccharide 2.0 ~ 20% feed additives.

Experimental Example 1: Feed efficiency improvement effect of the composition for animal feed additives of the present invention on weaning piglets

The composition for animal feed additives of the present invention prepared in Example 1 was added to the weaning pig feed 0.5% by 28 days to 56 days old weaning pigs were fed for 28 days and the daily weight gain was measured. At this time, the control group was added to 20ppm Avilacin + 110ppm CTC + 100ppm Sulfathiazole (Sulfathiazole) to feed the weaned pigs and the daily weight gain was measured and compared. As a result, as shown in Table 5, there was no significant difference between the control and the daily gain and feed efficiency. This indicates that the composition for animal feed additives of the present invention has a sufficient substitution effect on the existing antibiotics.

Effect of the composition for livestock feed on weaning piglet growth Item Control ¹ Inventive Composition ² Livestock 30 30 Initial weight (kg) 7.72 7.94 Late weight (kg) 18.17 17.78 ADFI ³ (kg) 0.56 0.54 ADG ⁴ (kg) 0.373 0.353 Salary: Increase (kg / kg) 1.53 1.55 [Note] 1: Feeding group containing 20ppm Avilacin + 110ppm CTC + 100ppm Sulfathiazole 2: 0.5% Dietary 3: Average daily feed 4: Average daily increase

Experimental Example 2: Feed efficiency improvement effect of the present invention for animal feed additive composition for piglets

The composition for animal feed additives prepared in Example 1 was added to feed to feed pigs for 21 days, and the daily gain and feed intake were examined. In the control group, 20 ppm of aviamycin + 110 ppm of CTC + 100 ppm of sulfathiazole were added to the feed, and the daily weight gain and feed intake were measured. As a result, as shown in Table 6, there was no significant difference with the control, but the daily gain, feed intake and feed efficiency were superior to the control with antibiotics.

Effect of the Invention for Livestock Feed Composition on Pig Growth Item Control ¹ Inventive Composition ² Livestock 30 30 Initial weight (kg) 10.06 10.05 Late weight (kg) 20.16 20.55 ADFI (kg) 0.742 0.768 ADG (kg) 0.481 0.500 Salary: Increase (kg / kg) 1.542 1.536 Note 1: Feed supplement group containing 20 ppm Avilacin + 110 ppm CTC + 100 ppm Sulfathiazole. 2: 0.5% diet

Experimental Example 3: Feed efficiency improvement effect of the composition for animal feed additives of the present invention on laying hens

The composition for animal feed additives prepared in Example 1 was added 0.5% to the feed and fed to laying hens for 21 days, and before and after feeding, egg production rate, eggshell quality, king egg production rate, egg weight, eggshell strength were compared. As a result of the experiment, as shown in Table 7, the group fed the feed composition of the present invention feed additives to the feed all showed a significant improvement.

Effect of the Invention for Animal Feed Additives on Laying Hens Item Before pay After salary Livestock 10,000 10,000 Egg production rate (%) 87,66 88.22 Eggshell (%) 1.04 0.80 Wang Specialty Egg (> 71g) Production Rate (%) 89.75 91.38 Egg weight (g) 67.3 71.2 Eggshell thickness (mm) 33.0 38.7 Eggshell Strength (kg) 3.60 4.80

Experimental Example 4: Feed efficiency improvement effect of the composition for animal feed additives of the present invention on lactating cows

The composition for animal feed additives of the present invention prepared in Example 1 was added to feed for 0.5 days and fed to lactating cows for 28 days, and the milk production and milk fat content were examined and compared with the control. It is an experimental group without adding. In addition, by adding the composition for animal feed additive 0.5% to the feed in the same manner compared to before feeding the somatic cell number of the group fed to lactating cows. As a result of the experiment, as shown in Table 8, the feed composition of the present invention was added to the feed and fed the milk yield, milk fat content increased and somatic cell count was greatly reduced. In addition, the composition of the present invention was added to feed and fed to the feed and compared with before the results shown in Table 9, the somatic cell count on day 7 and day 14 after the reduction was 29% and 43.6% respectively.

Effect of the Invention for Livestock Feed Additives on Beef Cattle Item Control Inventive Composition ¹ Livestock 27 27 Milk output (kg) 24.3 24.5 Maintenance fat (%) 4.2 4.5 Somatic cell count (× 10 ⁴ ) 80.3 57.9 Note 1: 0.5% Diet

Effect of the Invention for Livestock Feed Additives on Beef Livestock Somatic cell count (× 10 ³ ) Before pay 7 days after salary 14 days after salary 20 2,792 1,975 (29.0%) 1,569 (43.6%)

Experimental Example 5: Feed efficiency improvement effect of the composition for animal feed additives of the present invention on the finishing pigs

The composition for animal feed additives prepared in Example 1 was added to feed to feed the pigs, the carcass rate and back fat thickness were measured, and the carcass grade was determined. For comparison, the carcass ratio and backfat thickness were measured and the carcass grades were compared between the group fed with 0.05% chlortetracycline and the basic diet. As a result, as shown in Table 10, when the group fed the antibiotic-added and antibiotic-free feed and the group fed the feed containing the animal feed additive composition of the present invention, the carcass ratio was improved while the backfat thickness was increased. Reduced, and the conductor grade was also excellent.

Effect of the Invention for Livestock Feed Additives on Pig Carcass Characteristics Item Control Invention Composition Antibiotic Additives ¹ Antibiotic Free ² In hog pigs (kg) 102.31 98.77 100.46 Conductor weight (kg) 76.80 73.44 75.78 Conductor rate 75.05 74.38 75.46 Back Fat ³ (cm) 2.53 2.37 2.34 Grade ⁴ 1.93 2.06 2.00 Note 1: Basic diet + 0.05% chlortetracycline 2: Basic diet 3: Back fat thickness at 10th rib 4: Grade A = 1, B = 2, C = 3, D = 4

Experimental Example 6: Effect of the present invention composition for animal feed additives on the enteric beneficial bacteria

The composition for animal feed additives of the present invention prepared in Example 1 was added to feed to feed 40 mice, and Lactobacillus acidopillus , an intestinal useful microorganism, was collected from the feces of the mice and counted. The control group was made into the group fed without adding the composition for animal feed additives of the present invention to the feed. As a result of the experiment, as shown in Table 11, the population of L. acidophillus, L. acidophillus, which is an enteric beneficial bacterium, was significantly increased in the case of the control group, while that of the control group was significantly decreased. Improves the growth of intestinal useful microorganisms.

Effect of the Invention on the Growth of Intestinal Beneficial Bacteria Payroll date Number of Lactobacillus ashidophilus (× 10 ⁸ ) Control Invention Composition 0 2.61 2.61 6 0.49 2.06 12 0.12 5.49 18 0.09 8.30 24 0.03 15.14

Experimental Example 7: Effect of the present invention composition for animal feed additives on the mortality of typhoid

The composition for animal feed additives prepared in Example 1 was added to feed and fed to 150,000 laying hens, and the typhoid mortality was examined and compared with the typhoid mortality before feeding. As a result, as shown in Fig. 1, 8 days after the start of the salary, the mortality caused by poultry typhoid was reduced to 40% before the salary.

Experimental Example 8: Cholesterol lowering effect in cow's blood of the composition for animal feed additives of the present invention

The composition for animal feed additives of the present invention was added 0.5% to the feed and fed to 40 cows for 7 days, and the content of HDL and LDL in the blood was measured and compared with the control. At this time, the control group is the experimental group without adding the composition for animal feed additives of the present invention. As a result, as shown in Table 12, the content of HDL-cholesterol in blood was significantly increased while the content of LDL-cholesterol was significantly decreased.

Effect of the Invention for Livestock Feed Additives on Cholesterol Content in Cow's Blood Item Control Invention Composition HDL-cholesterol (mg / dL) 114 137 LDL-cholesterol (mg / dL) 141 111

Experimental Example 9: Cholesterol lowering effect of laying hens muscle composition of the present invention feed additive composition

The composition for animal feed additives of the present invention was added to the feed 0.5% for broiler and 100 laying hens for 18 days and the intramuscular cholesterol content was measured and compared with the control. At this time, the control group is the experimental group without adding the composition for animal feed additives of the present invention. As a result, as shown in Table 13, the content of cholesterol in muscle was significantly decreased in broiler and the content of cholesterol in egg yolk was significantly decreased in laying hens.

Effect of the Invention for Animal Feed Additives on Cholesterol Content in Laying Hens Item Cholesterol (mg / 100g) Control Invention Composition Broiler muscle 96.3 64.5 skin 118.6 78.4 Laying hen Yolk 1,350 584.1

Experimental Example 10 Antimicrobial Effect of the Invention for Livestock Feed Composition

Antibacterial effect was measured by paper disc (filter paper 8mm, Watman) method. The sterilized medium was prepared, cooled to about 35 ° C., and 1 mL of bacteria in a broth state were added thereto, mixed evenly to prevent foaming, and then dispensed about 20 mL in a petri dish. When the medium is hardened, sterile paper disc (8mm) is soaked in the test solution at a certain concentration and placed on it using tweezers, followed by incubation for 24 hours in an incubator at 37 ° C to the size of the inhibitory ring around the paper disc. The intensity of the activity was measured. The control group (Con) was compared with the antimicrobial effect using 0.1M acetate buffer of pH 5.0. Sample concentration setting was performed by dividing the chitosan concentration into four types. That is, C-Ⅰ is an experimental group containing 0.1% chitosan as an experimental group containing the composition for animal feed additives of the present invention, and C-II is an experimental group containing 0.2% chitosan as an experimental group including the composition for the animal feed additives of the present invention, C- III is an experimental group containing 0.3% chitosan as an experimental group containing the composition for animal feed additives of the present invention, and C-IV is an experimental group in which 0.4% chitosan was added to the experimental group where the composition for animal feed additives was tested. Test bacteria were obtained from E. coli 0157 (Korea Food Research Institute).E. coli0157), Salmonella typhimurium (Salmonella typimurium), Listeria monocytogenes (Listeria monocytogenes) E. coli 0157 (E. coliO157): Nutrient broth + Agar 0.7%, Salmonella typhimurium (Salmonella typimurium)silver Nutrient broth + Agar 0.7%, Listeria monocytogenesListeria monocytogenes) Used Brain heart infusion + Agar 0.7%, respectively. As a result of the experiment, E. coli 0157 (E. coliO157) and Salmonella typhimurium (Salmonella typimurium), The inhibitory ring was clearly seen.

As described above through the above examples and experimental examples, crude protein (crude protein) 15 to 25%, crude fat (crude fat) 5 to 15%, crude fiber (crude fiber) 7-14%, crude ash (crude ash) 17 to 30%, moisture 0 to 18%, calcium 2 to 5%, phosphorus 1 to 3%, mannan 0 to 4%, glucan 0 to 4%, Amylase (amylase) 0 ~ 30,000unit / kg, carotenoid-based antioxidant 0.00001 ~ 0.0001%, chitin and chitosan 2.0 ~ 20%, chitosan oligosaccharide composition containing the present invention for animal feed additive composition is antimicrobial action and immune enhancement It has the effect of promoting the growth of livestock, promoting the growth of fat, increasing the intestinal beneficial bacteria, reducing the amount of cholesterol, and improving egg shell and egg yellow color in laying hens, and producing high quality milk in lactating cows. In the livestock and livestock feed industry Wu will be useful inventions.

Claims (2)

Animal feed additives Composition for animal feed additives containing 2.0 to 20% chitosan and chitosan oligosaccharide 2.0 to 20% of the total composition Crude protein 15-25%, crude fat 5-15%, crude fiber 7-14%, crude ash 17-30%, moisture 0-18%, Calcium 2 ~ 5%, Phosphorus 1 ~ 3%, Mannan 0 ~ 4%, Glucan 0 ~ 4%, Amylase 0 ~ 30,000unit / kg, Carotenoid antioxidant A composition for animal feed additives comprising 0.00001 to 0.0001%, chitin and chitosan 2.0 to 20%, and chitosan oligosaccharide 2.0 to 20%.