KR102818627B1 - 융합 단백질 합성용 방법 및 생성물 - Google Patents

융합 단백질 합성용 방법 및 생성물 Download PDF

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KR102818627B1
KR102818627B1 KR1020187000488A KR20187000488A KR102818627B1 KR 102818627 B1 KR102818627 B1 KR 102818627B1 KR 1020187000488 A KR1020187000488 A KR 1020187000488A KR 20187000488 A KR20187000488 A KR 20187000488A KR 102818627 B1 KR102818627 B1 KR 102818627B1
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protein
peptide linker
amino acid
seq
lys
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KR20180050640A (ko
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마크 호왈쓰
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옥스포드 유니버시티 이노베이션 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Pulmonology (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020187000488A 2015-06-05 2016-06-03 융합 단백질 합성용 방법 및 생성물 Active KR102818627B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1509782.7A GB201509782D0 (en) 2015-06-05 2015-06-05 Methods and products for fusion protein synthesis
GB1509782.7 2015-06-05
PCT/GB2016/051640 WO2016193746A1 (en) 2015-06-05 2016-06-03 Methods and products for fusion protein synthesis

Publications (2)

Publication Number Publication Date
KR20180050640A KR20180050640A (ko) 2018-05-15
KR102818627B1 true KR102818627B1 (ko) 2025-06-10

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KR1020187000488A Active KR102818627B1 (ko) 2015-06-05 2016-06-03 융합 단백질 합성용 방법 및 생성물

Country Status (11)

Country Link
US (1) US10526379B2 (enExample)
EP (1) EP3303374B1 (enExample)
JP (1) JP6883529B2 (enExample)
KR (1) KR102818627B1 (enExample)
CN (2) CN116199733A (enExample)
AU (1) AU2016272543B2 (enExample)
CA (1) CA2987821A1 (enExample)
DK (1) DK3303374T3 (enExample)
ES (1) ES2880336T3 (enExample)
GB (1) GB201509782D0 (enExample)
WO (1) WO2016193746A1 (enExample)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016112921A1 (en) 2015-01-15 2016-07-21 University Of Copenhagen Virus-like particle with efficient epitope display
GB201509782D0 (en) * 2015-06-05 2015-07-22 Isis Innovation Methods and products for fusion protein synthesis
ES2854726T3 (es) 2015-10-30 2021-09-22 The Univ Of Copenhagen Partícula similar a virus con presentación eficiente de epítopos
WO2018170362A2 (en) * 2017-03-16 2018-09-20 The Penn State Research Foundation Versatile display scaffolds for proteins
GB201705750D0 (en) * 2017-04-10 2017-05-24 Univ Oxford Innovation Ltd Peptide ligase and use therof
GB201706430D0 (en) 2017-04-24 2017-06-07 Univ Oxford Innovation Ltd Proteins and peptide tags with enhanced rate of spontaneous isopeptide bond formation and uses thereof
CN107299107A (zh) * 2017-07-31 2017-10-27 北京大学 一种蛋白质层层组装功能材料的制备方法
EP4524157A3 (en) 2017-10-25 2025-06-18 Abera Bioscience AB Omv display
US11453883B2 (en) 2018-04-05 2022-09-27 Bio-Rad Abd Serotec Gmbh Display systems for proteins of interest
SG11202010821TA (en) 2018-05-04 2020-11-27 Spybiotech Ltd Vaccine composition
US12360021B2 (en) 2018-08-20 2025-07-15 The Regents Of The University Of California Graphene oxide affinity sample grids for Cyro-EM
CN111073925B (zh) * 2018-10-19 2022-04-26 北京大学 一种基于无序蛋白偶联酶的高效多肽-多肽偶联系统和方法
GB201819850D0 (en) 2018-12-05 2019-01-23 Univ Oxford Innovation Ltd Polypeptide and its use in affinity purification
GB201903479D0 (en) 2019-03-14 2019-05-01 Univ Oxford Innovation Ltd Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof
WO2020188346A1 (en) 2019-03-18 2020-09-24 Bio-Rad Abd Serotec Gmbh Antigen binding proteins
WO2020188356A1 (en) 2019-03-18 2020-09-24 Bio-Rad Abd Serotec Gmbh Antigen binding fragments conjugated to a plurality of fc isotypes and subclasses
WO2020188350A1 (en) 2019-03-18 2020-09-24 Bio-Rad Abd Serotec Gmbh Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation
US12163164B2 (en) 2019-07-15 2024-12-10 Bio-Rad Laboratories, Inc. Hybrid reverse transcriptases
NL2023863B1 (en) 2019-09-20 2021-05-25 Univ Griffith Protein particles and uses thereof
CN112646800B (zh) * 2019-10-11 2025-10-17 北京科技大学 制备固定化酶或固定化蛋白质的方法以及固定化酶或固定化蛋白质
GB201915905D0 (en) 2019-11-01 2019-12-18 Spybiotech Ltd Viruses with modified capsid proteins
EP3831843A1 (en) 2019-12-08 2021-06-09 Royal College Of Surgeons In Ireland A hemostatic agent and uses thereof
US20240294585A1 (en) * 2020-04-13 2024-09-05 Colin David Gottlieb Modular binding proteins for extracellular vesicles and uses thereof
CA3177709A1 (en) * 2020-05-07 2021-11-11 Adaptvac Aps Peptide tags and binding partners
US12298314B2 (en) 2020-10-09 2025-05-13 10X Genomics, Inc. Methods and compositions for analyzing target binding of molecules
WO2022076914A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for profiling immune repertoire
GB202019817D0 (en) 2020-12-15 2021-01-27 Univ Oxford Innovation Ltd Ligand-binding polypeptides and uses thereof
GB202104104D0 (en) 2021-03-24 2021-05-05 Liliumx Ltd Platform and method
GB202104999D0 (en) 2021-04-08 2021-05-26 Univ Oxford Innovation Ltd Polypeptides that interact with peptide tags at loops or termini and uses thereof
CN113621031B (zh) * 2021-04-23 2023-05-05 中山大学 一种利用自发异肽键进行蛋白共价自组装的肽链接头的组合
GB202106361D0 (en) 2021-05-04 2021-06-16 Spybiotech Ltd Viral vectored vaccines
US20240240203A1 (en) 2021-05-04 2024-07-18 SpyBiotech Limited Adenoviral vectors and vaccines thereof
US20250129132A1 (en) * 2021-09-21 2025-04-24 University Of Washington Genetically encoded and exogenously triggered protein-protein ligation
CN113862235B (zh) * 2021-09-24 2024-07-05 苏州近岸蛋白质科技股份有限公司 一种嵌合酶及其在体外一步反应合成Cap0 mRNA的用途和方法
GB202117283D0 (en) 2021-11-30 2022-01-12 Univ Oxford Innovation Ltd Switchable polypeptide and its use for gentle affinity purification
US20250009853A1 (en) 2021-12-03 2025-01-09 Wisconsin Alumni Research Foundation Mutant ace2 proteins and methods of using same
US12043862B2 (en) 2021-12-28 2024-07-23 Encodia, Inc. High-throughput serotyping and antibody profiling assays
CN114672505A (zh) * 2022-03-15 2022-06-28 江苏省中国科学院植物研究所 一种功能性表达细胞色素p450酶的方法及其应用
CN114732898B (zh) * 2022-04-01 2023-05-09 中国人民解放军军事科学院军事医学研究院 一种CpG佐剂与抗原定点共价结合方法
WO2024006532A2 (en) * 2022-06-30 2024-01-04 La Jolla Institute For Immunology Kari nanoparticle
CN116284278B (zh) * 2022-07-22 2025-10-03 南京工业大学 一种高酯键形成效率分子肽突变体
EP4590626A2 (en) * 2022-09-19 2025-07-30 The Rockefeller University Affinity capturing and directly determining structures of proteins and other materials on superparamagnetic beads by cryo-electron microscopy single-particle analysis
EP4594344A2 (en) 2022-09-28 2025-08-06 Valink Therapeutics Ltd Multivalent proteins and screening methods
CN116047055A (zh) * 2022-11-18 2023-05-02 常州伯仪生物科技有限公司 一种大规模定向标记碱性磷酸酶的方法
CN116165377A (zh) * 2023-04-21 2023-05-26 常州伯仪生物科技有限公司 一种通过竞争elisa法进行标签蛋白定量的方法
GB202306808D0 (en) 2023-05-09 2023-06-21 Univ Oxford Innovation Ltd Method for detecting interactions
GB202313175D0 (en) 2023-08-30 2023-10-11 Univ Oxford Innovation Ltd A bivalent polypeptide scaffold and uses thereof
US20250122482A1 (en) 2023-09-07 2025-04-17 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
GB202315107D0 (en) 2023-10-02 2023-11-15 Univ Oxford Innovation Ltd Polypeptides and peptide tags capable of spontaneous isopeptide bond formation at low temperature and low pH and uses thereof
US20250163468A1 (en) 2023-11-21 2025-05-22 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus
GB202317925D0 (en) 2023-11-23 2024-01-10 Spybiotech Ltd Vaccines
WO2025135040A1 (ja) * 2023-12-18 2025-06-26 公立大学法人大阪 イソペプチド結合形成による新規ポリペプチドの作製方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011098772A1 (en) 2010-02-11 2011-08-18 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
WO2012127060A1 (fr) 2011-03-24 2012-09-27 Commissariat à l'énergie atomique et aux énergies alternatives Peptides aptes à former un complexe covalent et leurs utilisations
WO2012142113A2 (en) 2011-04-11 2012-10-18 The Uab Research Foundation Isopeptide bond formation in bacillus species and uses thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU530410B2 (en) 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
ATE361365T1 (de) * 1998-07-27 2007-05-15 Sanofi Pasteur Ltd Streptococcus pneumoniae proteine und nukleinsäuren
US6936252B2 (en) * 1998-07-27 2005-08-30 Microbial Technics Limited Streptococcus pneumoniae proteins and nucleic acid molecules
DE10108263A1 (de) * 2001-02-21 2002-09-05 Max Planck Gesellschaft Analyse von Modifizierungen und Demodifizierungen von Proteinen mit Ubiquitinverwandten Proteinen mittels FRET (Fluorescence Resonance Energy Transfer)
WO2004031243A1 (ja) * 2002-10-01 2004-04-15 Kumamoto Technology & Industry Foundation タンパク質ポリマー及びその製造方法
ATE537183T1 (de) * 2006-02-02 2011-12-15 Trimeris Inc Hiv-fusionshemmerpeptide mit verbesserten biologischen eigenschaften
BRPI0811908A2 (pt) * 2007-05-25 2014-11-18 Novartis Ag Antígenos de pilus de streptococcus pneumoniae
GB0714963D0 (en) * 2007-08-01 2007-09-12 Novartis Ag Compositions comprising antigens
US20090253131A1 (en) * 2007-11-05 2009-10-08 Promega Corporation Hybrid fusion reporter and uses thereof
EP3008094B1 (en) 2013-06-14 2018-12-26 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US10073087B2 (en) 2014-01-15 2018-09-11 Massachusetts Institute Of Technology Biopolymer-mediated assembly of nanoparticles using genetically encoded proteins
GB201509782D0 (en) * 2015-06-05 2015-07-22 Isis Innovation Methods and products for fusion protein synthesis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011098772A1 (en) 2010-02-11 2011-08-18 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
WO2012127060A1 (fr) 2011-03-24 2012-09-27 Commissariat à l'énergie atomique et aux énergies alternatives Peptides aptes à former un complexe covalent et leurs utilisations
WO2012142113A2 (en) 2011-04-11 2012-10-18 The Uab Research Foundation Isopeptide bond formation in bacillus species and uses thereof

Also Published As

Publication number Publication date
GB201509782D0 (en) 2015-07-22
AU2016272543A1 (en) 2018-01-04
CA2987821A1 (en) 2016-12-08
US20180244730A1 (en) 2018-08-30
JP2018521640A (ja) 2018-08-09
ES2880336T3 (es) 2021-11-24
CN108026148A (zh) 2018-05-11
AU2016272543B2 (en) 2020-08-13
JP6883529B2 (ja) 2021-06-09
KR20180050640A (ko) 2018-05-15
EP3303374B1 (en) 2021-03-31
WO2016193746A1 (en) 2016-12-08
DK3303374T3 (da) 2021-07-05
CN116199733A (zh) 2023-06-02
BR112017026042A2 (pt) 2018-08-14
US10526379B2 (en) 2020-01-07
EP3303374A1 (en) 2018-04-11
CN108026148B (zh) 2022-12-30

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