BR112017026042A2 - métodos e produtos para síntese de proteína de fusão - Google Patents
métodos e produtos para síntese de proteína de fusãoInfo
- Publication number
- BR112017026042A2 BR112017026042A2 BR112017026042A BR112017026042A BR112017026042A2 BR 112017026042 A2 BR112017026042 A2 BR 112017026042A2 BR 112017026042 A BR112017026042 A BR 112017026042A BR 112017026042 A BR112017026042 A BR 112017026042A BR 112017026042 A2 BR112017026042 A2 BR 112017026042A2
- Authority
- BR
- Brazil
- Prior art keywords
- protein
- ligands
- peptide
- bound
- pair
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Pulmonology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
a presente invenção refere-se a um método para produzir uma proteína de fusão, em que o dito método compreende: a) colocar uma primeira proteína em contato com uma segunda proteína em condições que permitem a formação de uma ligação de isopeptídeo entre as ditas proteínas, em que a dita primeira proteína e a dita segunda proteína compreendem, cada uma, um ligante de peptídeo, em que os ditos ligantes de peptídeo são um par de ligantes de peptídeo que reage para formar uma ligação de isopeptídeo que liga a dita primeira proteína à dita segunda proteína para formar uma proteína ligada; e b) colocar a proteína ligada de (a) em contato com uma terceira proteína em condições que permitem a formação de uma ligação de isopeptídeo entre a dita terceira proteína e a dita proteína ligada, em que a dita terceira proteína compreende um ligante de peptídeo que reage com um ligante de peptídeo adicional na proteína ligada de (a) e em que os ditos ligantes de peptídeo são um par de ligantes de peptídeo que reage para formar uma ligação de isopeptídeo que liga a dita terceira pro-teína à dita proteína ligada para formar uma proteína de fusão, em que o dito par de ligantes de peptídeo usado em (a) é ortogonal ao par de ligantes de peptídeo usado em (b). os ligantes de peptídeo e o uso de pares ortogonais dos ditos ligantes na síntese de proteínas de fusão também são fornecidos. as proteínas recombinantes que compreendem os ditos ligantes, as moléculas de ácido nucleico que codificam as ditas proteínas e os ligantes, os vetores que compreendem as ditas moléculas de ácido nucleico e as células hospedeiras que compreendem os ditos vetores e as ditas moléculas de ácido nucleico também são contemplados.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1509782.7A GB201509782D0 (en) | 2015-06-05 | 2015-06-05 | Methods and products for fusion protein synthesis |
| PCT/GB2016/051640 WO2016193746A1 (en) | 2015-06-05 | 2016-06-03 | Methods and products for fusion protein synthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017026042A2 true BR112017026042A2 (pt) | 2018-08-14 |
Family
ID=53785013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017026042A BR112017026042A2 (pt) | 2015-06-05 | 2016-06-03 | métodos e produtos para síntese de proteína de fusão |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10526379B2 (pt) |
| EP (1) | EP3303374B1 (pt) |
| JP (1) | JP6883529B2 (pt) |
| KR (1) | KR20180050640A (pt) |
| CN (2) | CN108026148B (pt) |
| AU (1) | AU2016272543B2 (pt) |
| BR (1) | BR112017026042A2 (pt) |
| CA (1) | CA2987821A1 (pt) |
| DK (1) | DK3303374T3 (pt) |
| ES (1) | ES2880336T3 (pt) |
| GB (1) | GB201509782D0 (pt) |
| WO (1) | WO2016193746A1 (pt) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2016112921A1 (en) | 2015-01-15 | 2016-07-21 | University Of Copenhagen | Virus-like particle with efficient epitope display |
| DK3368068T3 (da) | 2015-10-30 | 2021-02-22 | Univ Copenhagen | Viruslignende partikler med effektiv epitopdisplay |
| WO2018170362A2 (en) * | 2017-03-16 | 2018-09-20 | The Penn State Research Foundation | Versatile display scaffolds for proteins |
| GB201705750D0 (en) * | 2017-04-10 | 2017-05-24 | Univ Oxford Innovation Ltd | Peptide ligase and use therof |
| GB201706430D0 (en) | 2017-04-24 | 2017-06-07 | Univ Oxford Innovation Ltd | Proteins and peptide tags with enhanced rate of spontaneous isopeptide bond formation and uses thereof |
| CN107299107A (zh) * | 2017-07-31 | 2017-10-27 | 北京大学 | 一种蛋白质层层组装功能材料的制备方法 |
| EP3701009A1 (en) | 2017-10-25 | 2020-09-02 | Abera Bioscience AB | Display of heterologous molecules on bacterial cells and membrane vesicles |
| EP3774926A1 (en) | 2018-04-05 | 2021-02-17 | Bio-Rad ABD Serotec GmbH | Display systems for proteins of interest |
| AU2019263706A1 (en) | 2018-05-04 | 2020-11-19 | SpyBiotech Limited | Vaccine composition |
| WO2020041202A1 (en) * | 2018-08-20 | 2020-02-27 | The Regents Of The University Of California | Graphene oxide affinity sample grids for cryo-em |
| CN111073925B (zh) * | 2018-10-19 | 2022-04-26 | 北京大学 | 一种基于无序蛋白偶联酶的高效多肽-多肽偶联系统和方法 |
| GB201819850D0 (en) | 2018-12-05 | 2019-01-23 | Univ Oxford Innovation Ltd | Polypeptide and its use in affinity purification |
| GB201903479D0 (en) * | 2019-03-14 | 2019-05-01 | Univ Oxford Innovation Ltd | Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof |
| CN113993889A (zh) * | 2019-03-18 | 2022-01-28 | 生物辐射Abd瑟罗泰克有限公司 | 与多个Fc同种型和亚类偶联的抗原结合片段 |
| US20200299358A1 (en) * | 2019-03-18 | 2020-09-24 | Bio-Rad Abd Serotec Gmbh | Antigen binding proteins |
| US11674164B2 (en) | 2019-03-18 | 2023-06-13 | Bio-Rad Abd Serotec Gmbh | Periplasmic fusion proteins |
| NL2023863B1 (en) | 2019-09-20 | 2021-05-25 | Univ Griffith | Protein particles and uses thereof |
| GB201915905D0 (en) | 2019-11-01 | 2019-12-18 | Spybiotech Ltd | Viruses with modified capsid proteins |
| EP3831843A1 (en) | 2019-12-08 | 2021-06-09 | Royal College Of Surgeons In Ireland | A hemostatic agent and uses thereof |
| EP4135665A2 (en) * | 2020-04-13 | 2023-02-22 | Mantra Bio, Inc. | Modular binding proteins for extracellular vesicles and uses thereof |
| IL297111A (en) * | 2020-05-07 | 2022-12-01 | Adaptvac Aps | Peptide labels and binding partners |
| WO2022076914A1 (en) | 2020-10-09 | 2022-04-14 | 10X Genomics, Inc. | Methods and compositions for profiling immune repertoire |
| GB202019817D0 (en) | 2020-12-15 | 2021-01-27 | Univ Oxford Innovation Ltd | Ligand-binding polypeptides and uses thereof |
| GB202104104D0 (en) * | 2021-03-24 | 2021-05-05 | Liliumx Ltd | Platform and method |
| GB202104999D0 (en) | 2021-04-08 | 2021-05-26 | Univ Oxford Innovation Ltd | Polypeptides that interact with peptide tags at loops or termini and uses thereof |
| CN113621031B (zh) * | 2021-04-23 | 2023-05-05 | 中山大学 | 一种利用自发异肽键进行蛋白共价自组装的肽链接头的组合 |
| GB202106361D0 (en) | 2021-05-04 | 2021-06-16 | Spybiotech Ltd | Viral vectored vaccines |
| WO2022234276A1 (en) | 2021-05-04 | 2022-11-10 | SpyBiotech Limited | Adenoviral vectors and vaccines thereof |
| WO2023049774A1 (en) * | 2021-09-21 | 2023-03-30 | University Of Washington | Genetically encoded and exogenously triggered protein-protein ligation |
| CN113862235A (zh) * | 2021-09-24 | 2021-12-31 | 苏州近岸蛋白质科技股份有限公司 | 一种嵌合酶及其在体外一步反应合成Cap0 mRNA的用途和方法 |
| GB202117283D0 (en) | 2021-11-30 | 2022-01-12 | Univ Oxford Innovation Ltd | Switchable polypeptide and its use for gentle affinity purification |
| WO2023102156A1 (en) | 2021-12-03 | 2023-06-08 | Wisconsin Alumni Research Foundation | Mutant ace2 proteins and methods of using same |
| US20230203562A1 (en) | 2021-12-28 | 2023-06-29 | Encodia, Inc. | High-throughput serotyping and antibody profiling assays |
| CN114672505A (zh) * | 2022-03-15 | 2022-06-28 | 江苏省中国科学院植物研究所 | 一种功能性表达细胞色素p450酶的方法及其应用 |
| CN114732898B (zh) * | 2022-04-01 | 2023-05-09 | 中国人民解放军军事科学院军事医学研究院 | 一种CpG佐剂与抗原定点共价结合方法 |
| WO2024006532A2 (en) * | 2022-06-30 | 2024-01-04 | La Jolla Institute For Immunology | Kari nanoparticle |
| WO2024064112A2 (en) * | 2022-09-19 | 2024-03-28 | The Rockefeller University | Affinity capturing and directly determining structures of proteins and other materials on superparamagnetic beads by cryo-electron microscopy single-particle analysis |
| WO2024069180A2 (en) | 2022-09-28 | 2024-04-04 | LiliumX Ltd. | Multivalent proteins and screening methods |
| CN116165377A (zh) * | 2023-04-21 | 2023-05-26 | 常州伯仪生物科技有限公司 | 一种通过竞争elisa法进行标签蛋白定量的方法 |
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| AU530410B2 (en) | 1978-02-21 | 1983-07-14 | Sintef | Preparing aqueous emulsions |
| US6936252B2 (en) * | 1998-07-27 | 2005-08-30 | Microbial Technics Limited | Streptococcus pneumoniae proteins and nucleic acid molecules |
| DE69935986D1 (de) * | 1998-07-27 | 2007-06-14 | Sanofi Pasteur Ltd | Streptococcus pneumoniae proteine und nukleinsäuren |
| DE10108263A1 (de) * | 2001-02-21 | 2002-09-05 | Max Planck Gesellschaft | Analyse von Modifizierungen und Demodifizierungen von Proteinen mit Ubiquitinverwandten Proteinen mittels FRET (Fluorescence Resonance Energy Transfer) |
| WO2004031243A1 (ja) * | 2002-10-01 | 2004-04-15 | Kumamoto Technology & Industry Foundation | タンパク質ポリマー及びその製造方法 |
| AR059300A1 (es) * | 2006-02-02 | 2008-03-26 | Trimeris Inc | Peptidos inhibidores de la fusion de vih con propiedades biologicas mejoradas |
| US20110097360A1 (en) * | 2007-05-25 | 2011-04-28 | Claudio Donati | Streptococcus pneumoniae pilus antigens |
| GB0714963D0 (en) * | 2007-08-01 | 2007-09-12 | Novartis Ag | Compositions comprising antigens |
| WO2009061413A2 (en) * | 2007-11-05 | 2009-05-14 | Promega Corporation | Hybrid fusion reporter and uses thereof |
| GB201002362D0 (en) * | 2010-02-11 | 2010-03-31 | Isis Innovation | Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds |
| FR2973032A1 (fr) * | 2011-03-24 | 2012-09-28 | Commissariat Energie Atomique | Peptides aptes a former un complexe covalent et leurs utilisations |
| US20120259101A1 (en) * | 2011-04-11 | 2012-10-11 | Li Tan | Isopeptide Bond Formation in Bacillus Species and Uses Thereof |
| EP3008094B1 (en) | 2013-06-14 | 2018-12-26 | Pacific Biosciences Of California, Inc. | Bis-biotinylation tags |
| WO2015156870A2 (en) | 2014-01-15 | 2015-10-15 | Massachusetts Institute Of Technology | Biopolymer-mediated assembly of nanoparticles using genetically encoded proteins |
-
2015
- 2015-06-05 GB GBGB1509782.7A patent/GB201509782D0/en not_active Ceased
-
2016
- 2016-06-03 ES ES16728359T patent/ES2880336T3/es active Active
- 2016-06-03 EP EP16728359.7A patent/EP3303374B1/en active Active
- 2016-06-03 US US15/578,486 patent/US10526379B2/en active Active
- 2016-06-03 DK DK16728359.7T patent/DK3303374T3/da active
- 2016-06-03 JP JP2017563189A patent/JP6883529B2/ja active Active
- 2016-06-03 CN CN201680037511.8A patent/CN108026148B/zh active Active
- 2016-06-03 CA CA2987821A patent/CA2987821A1/en active Pending
- 2016-06-03 BR BR112017026042A patent/BR112017026042A2/pt unknown
- 2016-06-03 WO PCT/GB2016/051640 patent/WO2016193746A1/en active Application Filing
- 2016-06-03 AU AU2016272543A patent/AU2016272543B2/en active Active
- 2016-06-03 CN CN202211548024.1A patent/CN116199733A/zh active Pending
- 2016-06-03 KR KR1020187000488A patent/KR20180050640A/ko not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2016272543A1 (en) | 2018-01-04 |
| EP3303374B1 (en) | 2021-03-31 |
| DK3303374T3 (da) | 2021-07-05 |
| ES2880336T3 (es) | 2021-11-24 |
| US20180244730A1 (en) | 2018-08-30 |
| WO2016193746A1 (en) | 2016-12-08 |
| CN108026148B (zh) | 2022-12-30 |
| CN108026148A (zh) | 2018-05-11 |
| CN116199733A (zh) | 2023-06-02 |
| JP6883529B2 (ja) | 2021-06-09 |
| US10526379B2 (en) | 2020-01-07 |
| EP3303374A1 (en) | 2018-04-11 |
| KR20180050640A (ko) | 2018-05-15 |
| JP2018521640A (ja) | 2018-08-09 |
| AU2016272543B2 (en) | 2020-08-13 |
| CA2987821A1 (en) | 2016-12-08 |
| GB201509782D0 (en) | 2015-07-22 |
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