KR102738175B1 - 무효소 및 무증폭 시퀀싱 - Google Patents
무효소 및 무증폭 시퀀싱 Download PDFInfo
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- KR102738175B1 KR102738175B1 KR1020227022673A KR20227022673A KR102738175B1 KR 102738175 B1 KR102738175 B1 KR 102738175B1 KR 1020227022673 A KR1020227022673 A KR 1020227022673A KR 20227022673 A KR20227022673 A KR 20227022673A KR 102738175 B1 KR102738175 B1 KR 102738175B1
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- South Korea
- Prior art keywords
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- nucleic acid
- probe
- detectable label
- acid molecule
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/149—Sequential reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462082883P | 2014-11-21 | 2014-11-21 | |
| US62/082,883 | 2014-11-21 | ||
| PCT/US2015/061615 WO2016081740A1 (en) | 2014-11-21 | 2015-11-19 | Enzyme- and amplification-free sequencing |
| KR1020217027822A KR102417849B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027822A Division KR102417849B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20220100098A KR20220100098A (ko) | 2022-07-14 |
| KR102738175B1 true KR102738175B1 (ko) | 2024-12-04 |
Family
ID=54705918
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027822A Active KR102417849B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
| KR1020177016946A Active KR102105236B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
| KR1020227022673A Active KR102738175B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
| KR1020207011298A Active KR102298387B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027822A Active KR102417849B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
| KR1020177016946A Active KR102105236B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020207011298A Active KR102298387B1 (ko) | 2014-11-21 | 2015-11-19 | 무효소 및 무증폭 시퀀싱 |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20160194701A1 (enExample) |
| EP (4) | EP4029952B1 (enExample) |
| JP (4) | JP6674951B2 (enExample) |
| KR (4) | KR102417849B1 (enExample) |
| CN (2) | CN113403373B (enExample) |
| AU (3) | AU2015349870B2 (enExample) |
| CA (1) | CA2968376C (enExample) |
| ES (2) | ES2770055T3 (enExample) |
| SG (1) | SG11201704098VA (enExample) |
| WO (1) | WO2016081740A1 (enExample) |
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| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| ES2952728T3 (es) | 2011-12-22 | 2023-11-03 | Harvard College | Métodos para la detección de analitos |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| RU2690935C2 (ru) | 2013-06-04 | 2019-06-06 | Президент Энд Фэллоуз Оф Харвард Коллидж | Направляемая рнк регуляция транскрипции |
| EP4234716A3 (en) | 2013-06-25 | 2023-12-06 | Prognosys Biosciences, Inc. | Methods for determining spatial patterns of biological targets in a sample |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| ES3014093T3 (en) | 2014-07-30 | 2025-04-16 | Harvard College | Method for determining nucleic acids |
| EP4119677B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US10640816B2 (en) | 2015-07-17 | 2020-05-05 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
| SG10202107055SA (en) | 2015-07-17 | 2021-08-30 | Nanostring Technologies Inc | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| EP4613756A3 (en) | 2016-04-25 | 2025-11-12 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN109715825B (zh) * | 2016-05-16 | 2023-01-06 | 纳米线科技公司 | 用于检测样品中目标核酸的方法 |
| WO2017222453A1 (en) | 2016-06-21 | 2017-12-28 | Hauling Thomas | Nucleic acid sequencing |
| CN109923216B (zh) | 2016-08-31 | 2024-08-02 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| EP3507364A4 (en) | 2016-08-31 | 2020-05-20 | President and Fellows of Harvard College | METHODS OF GENERATING NUCLEIC ACID SEQUENCE LIBRARIES FOR IN SITU FLUORESCENT SEQUENCING DETECTION |
| JP6730525B2 (ja) * | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ，インコーポレイティド | 化学組成物とそれを利用する方法 |
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| KR20250161650A (ko) | 2018-02-12 | 2025-11-17 | 브루커 스페이셜 바이올로지, 인크. | 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법 |
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