AU2015349870B2 - Enzyme- and amplification-free sequencing - Google Patents

Enzyme- and amplification-free sequencing Download PDF

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Publication number
AU2015349870B2
AU2015349870B2 AU2015349870A AU2015349870A AU2015349870B2 AU 2015349870 B2 AU2015349870 B2 AU 2015349870B2 AU 2015349870 A AU2015349870 A AU 2015349870A AU 2015349870 A AU2015349870 A AU 2015349870A AU 2015349870 B2 AU2015349870 B2 AU 2015349870B2
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nucleic acid
detectable label
acid molecule
sequencing
target
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AU2015349870A1 (en
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Joseph M. Beechem
Rustem KHAFIZOV
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Bruker Spatial Biology Inc
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Bruker Spatial Biology Inc
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Priority to AU2022271472A priority patent/AU2022271472B2/en
Assigned to BRUKER SPATIAL BIOLOGY, INC. reassignment BRUKER SPATIAL BIOLOGY, INC. Request for Assignment Assignors: NANOSTRING TECHNOLOGIES, INC.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/149—Sequential reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2015349870A 2014-11-21 2015-11-19 Enzyme- and amplification-free sequencing Active AU2015349870B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2019275665A AU2019275665B2 (en) 2014-11-21 2019-12-06 Enzyme- and amplification-free sequencing
AU2022271472A AU2022271472B2 (en) 2014-11-21 2022-11-18 Enzyme- and amplification-free sequencing

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462082883P 2014-11-21 2014-11-21
US62/082,883 2014-11-21
PCT/US2015/061615 WO2016081740A1 (en) 2014-11-21 2015-11-19 Enzyme- and amplification-free sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2019275665A Division AU2019275665B2 (en) 2014-11-21 2019-12-06 Enzyme- and amplification-free sequencing

Publications (2)

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AU2015349870A1 AU2015349870A1 (en) 2017-06-08
AU2015349870B2 true AU2015349870B2 (en) 2019-09-26

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AU2015349870A Active AU2015349870B2 (en) 2014-11-21 2015-11-19 Enzyme- and amplification-free sequencing
AU2019275665A Active AU2019275665B2 (en) 2014-11-21 2019-12-06 Enzyme- and amplification-free sequencing
AU2022271472A Active AU2022271472B2 (en) 2014-11-21 2022-11-18 Enzyme- and amplification-free sequencing

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AU2022271472A Active AU2022271472B2 (en) 2014-11-21 2022-11-18 Enzyme- and amplification-free sequencing

Country Status (10)

Country Link
US (4) US20160194701A1 (enExample)
EP (4) EP4029952B1 (enExample)
JP (4) JP6674951B2 (enExample)
KR (4) KR102738175B1 (enExample)
CN (2) CN113403373B (enExample)
AU (3) AU2015349870B2 (enExample)
CA (1) CA2968376C (enExample)
ES (2) ES2910099T3 (enExample)
SG (1) SG11201704098VA (enExample)
WO (1) WO2016081740A1 (enExample)

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