KR102626620B1 - 임산부 자궁경부 탈락 세포로부터 태반 영양막 세포를 분리하는 방법 - Google Patents
임산부 자궁경부 탈락 세포로부터 태반 영양막 세포를 분리하는 방법 Download PDFInfo
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110549947.8 | 2021-05-20 | ||
| CN202110549947.8A CN112980779B (zh) | 2021-05-20 | 2021-05-20 | 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法 |
| PCT/CN2022/081234 WO2022242285A1 (zh) | 2021-05-20 | 2022-03-16 | 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法 |
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| Publication Number | Publication Date |
|---|---|
| KR20230004915A KR20230004915A (ko) | 2023-01-06 |
| KR102626620B1 true KR102626620B1 (ko) | 2024-01-17 |
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| KR1020227043777A KR102626620B1 (ko) | 2021-05-20 | 2022-03-16 | 임산부 자궁경부 탈락 세포로부터 태반 영양막 세포를 분리하는 방법 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980779B (zh) | 2021-05-20 | 2021-08-24 | 广州凯普医药科技有限公司 | 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726585A (zh) * | 2009-11-30 | 2010-06-09 | 宁波普赛微流科技有限公司 | 一种基于微流控芯片的流式细胞仪 |
| US20160090631A1 (en) * | 2013-06-11 | 2016-03-31 | James R. STELLING | Method for detection of fetal abnormalities |
| CN111304153A (zh) * | 2019-12-17 | 2020-06-19 | 广东凯普生物科技股份有限公司 | 一种分离滋养层细胞的方法 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE161960T1 (de) * | 1988-12-06 | 1998-01-15 | Flinders Technologies Pty Ltd | Isolierung von fetalen zellen aus mütterlichem blut zur ausführung von pränataler diagnostik |
| BR9306867A (pt) * | 1992-07-17 | 1998-12-08 | Aprogenex Inc | Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ |
| US5714325A (en) * | 1993-09-24 | 1998-02-03 | New England Medical Center Hospitals | Prenatal diagnosis by isolation of fetal granulocytes from maternal blood |
| JPH11503320A (ja) * | 1995-04-07 | 1999-03-26 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Ｈｌａ−ｇ検出用抗体 |
| US20020045196A1 (en) * | 2000-05-12 | 2002-04-18 | Walt Mahoney | Methods of isolating trophoblast cells from maternal blood |
| AUPR749901A0 (en) * | 2001-09-06 | 2001-09-27 | Monash University | Method of identifying chromosomal abnormalities and prenatal diagnosis |
| US20040197832A1 (en) * | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
| US20050181429A1 (en) * | 2003-04-03 | 2005-08-18 | Monaliza Medical Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
| WO2005047532A1 (en) * | 2003-11-17 | 2005-05-26 | Gribbles Molecular Science Pty Ltd | Improved method of performing genetic analyses on reproductive tract cell samples |
| US20070224597A1 (en) * | 2006-03-23 | 2007-09-27 | Biocept, Inc. | Isolating fetal trophoblasts |
| DE102007019017A1 (de) * | 2007-04-19 | 2009-01-22 | Ldt Laser Display Technology Gmbh | Verfahren und Vorrichtung zum Projizieren eines Bildes auf eine Projektionsfläche |
| ITTO20070307A1 (it) * | 2007-05-04 | 2008-11-05 | Silicon Biosystems Spa | Metodo e dispositivo per la diagnosi prenatale non-invasiva |
| EP2419507A1 (en) * | 2009-04-14 | 2012-02-22 | Institut Gustave Roussy | Prostate cancer cell lines and their use in screening method |
| AU2010239131A1 (en) * | 2009-04-21 | 2011-11-17 | Genetic Technologies Limited | Methods for obtaining fetal genetic material |
| WO2013174948A1 (en) * | 2012-05-24 | 2013-11-28 | Rarecells | Process for multi-analyses of rare cells extracted or isolated from biological samples through filtration |
| CA2888746C (en) * | 2012-10-19 | 2022-07-26 | Wayne State University | Identification and analysis of fetal trophoblast cells in cervical mucus for prenatal diagnosis |
| CN103983794B (zh) * | 2013-02-12 | 2016-06-15 | 李木 | 一种微流控芯片及一种微流控方法 |
| AU2017246480B2 (en) * | 2016-04-06 | 2023-06-01 | Wayne State University | Isolation and analysis of fetal DNA from extravillous trophoblast cells retrieved from the endocervical canal |
| CN107312747A (zh) * | 2016-12-20 | 2017-11-03 | 华东医药（杭州）基因科技有限公司 | 一种免疫磁珠法分离胎盘滋养层细胞的方法 |
| CN108265051B (zh) * | 2016-12-30 | 2020-08-25 | 张婧 | 用于从母体捕获和分离胎儿细胞的无创方法和试剂盒 |
| WO2019153032A1 (en) * | 2018-02-07 | 2019-08-15 | University Of South Australia | Pre-natal cell isolation |
| CN108535228B (zh) * | 2018-04-03 | 2020-12-25 | 德运康明(厦门)生物科技有限公司 | 一种从孕妇外周血中分离游离胎儿细胞的方法 |
| TWI668423B (zh) * | 2018-10-02 | 2019-08-11 | 吳宏偉 | 細胞分選方法及系統 |
| CN110117571B (zh) * | 2019-05-08 | 2023-01-31 | 南方医科大学南方医院 | 无创获取胎儿稀有细胞的试剂盒及其方法 |
| EP4242652A3 (en) * | 2019-06-07 | 2023-12-06 | Arcedi Biotech ApS | Isolation of fetal cells using facs |
| WO2021009682A1 (en) * | 2019-07-15 | 2021-01-21 | Menarini Biomarkers Singapore Pte Ltd | Compositions and methods for isolating, detecting, and analyzing fetal cells |
| CN111440696B (zh) * | 2020-02-26 | 2023-02-24 | 德运康明(厦门)生物科技有限公司 | 胎儿细胞捕获模块、用于胎儿细胞捕获的微流控芯片，及它们的使用方法 |
| CN112980779B (zh) | 2021-05-20 | 2021-08-24 | 广州凯普医药科技有限公司 | 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法 |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726585A (zh) * | 2009-11-30 | 2010-06-09 | 宁波普赛微流科技有限公司 | 一种基于微流控芯片的流式细胞仪 |
| US20160090631A1 (en) * | 2013-06-11 | 2016-03-31 | James R. STELLING | Method for detection of fetal abnormalities |
| CN111304153A (zh) * | 2019-12-17 | 2020-06-19 | 广东凯普生物科技股份有限公司 | 一种分离滋养层细胞的方法 |
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