KR102500758B1 - Method for preparing cosmetic composition containg liposomes containing five kinds of ceramides - Google Patents

Abstract

The present invention provides a manufacturing method of a cosmetic composition which stably collects active substances, has increased solubility and dispersibility, and contains a liposome including 5 types of ceramides consisting of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP. The cosmetic composition which contains the liposomes prepared according to the manufacturing method of the present invention has an advantage of being able to more stably collect active substances while having a small particle size, and having increased solubility and dispersibility.

Description

Manufacturing method of cosmetic composition containing liposomes including 5 types of ceramides

The present invention relates to a method for preparing a cosmetic composition containing liposomes containing 5 types of ceramides stably trapping active substances and increasing dissolving power and dispersing power.

The skin of the human body covers the outer surface of the body and is the primary defense system that serves to protect the human body from harmful external environments such as ultraviolet rays and bacteria. Due to this, the skin is continuously exposed to external stimuli and not only is damaged, but also its physiological function is lowered due to the natural aging process. Although these skin aging and damage processes inevitably occur as we age, measures to slow down skin aging and improve skin condition are required in modern society due to the improvement in the quality of living standards and the rise in interest in beauty. It is currently being demanded.

The skin is composed of three layers: epidermis, dermis, and subcutaneous fat, and the outermost epidermis plays the most important role in terms of skin aging and has been the subject of intensive research.

The components that make up the stratum corneum, the outermost layer of the epidermis, are keratinocytes and skin lipids. Skin lipids are responsible for the barrier function of the skin. This barrier function of the skin regulates cell division and differentiation to protect the skin from external harmful substances and , It can be said that it is an important function to prevent the leakage of substances in the body and the evaporation of skin moisture.

Among skin lipids, lipids responsible for the barrier function of the skin are mainly composed of ceramide, cholesterol, and free fatty acids, which account for 40-65%, 10%, and 25% of the total lipids in the stratum corneum, respectively.

In particular, ceramide, a component of the stratum corneum that protects the skin, loses its production ability with age, and is gradually broken down as it is exposed to external stimuli such as sunlight or fine dust for a long time. As a result, it does not function to protect the skin. There are problems that cause skin to become dry and accelerate various troubles, inflammation, wrinkles, and aging.

On the other hand, various studies are being conducted to improve these problems, and Korean Publication No. 10-2013-0023912 proposes a functional cosmetic composition stabilized by inclusion in nanoliposomes, and Korean Registration No. 10-2404209 A method for preparing liposome vesicles having an average size of 230 to 290 nm of liposome vesicles having excellent skin permeability is proposed.

Korean Publication No. 10-2013-0023912 Korean Registration No. 10-2404209

In the present invention, it is intended to provide a method for preparing a cosmetic composition containing ceramide-encapsulated liposomes having a small particle size with increased dissolving power and dispersing power while collecting active substances more stably.

In addition, the present invention is intended to provide a method for preparing a liposome-containing cosmetic composition that is easily absorbed into the skin, has a softer feeling of use and excellent formulation stability.

In order to achieve the above object, the present invention After mixing 5 types of ceramides consisting of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP, adding glycerin and 1,2-hexanediol, caprylic/capric triglyceride and baobab seed oil adding and dissolving under a temperature condition of 80° C. to obtain a primary mixture; adding hydrogenated lecithin and glycolipid to the first mixture and stirring at a rotational speed of 3,000 rpm for 1 hour under a temperature condition of 80° C. using a homomixer to obtain a second mixture; Purified water, tocopherol, phytosterol, and sodium hyaluronate were added to the secondary mixture, and agitated twice continuously under high-pressure conditions of 1,000 to 1,500 bar and temperature conditions of 80 ° C. using a high-pressure homomixer to form liposomes. obtaining a tea mixture; and filtering the tertiary mixture through a filter having a size of 0.45 μm to obtain liposomes having a size of less than 0.45 μm formed in the tertiary mixture; It provides a method for producing a liposome-containing cosmetic composition comprising a.

In one embodiment, based on the total weight of the cosmetic composition, 0.1% by weight of ceramide NP, 0.002% by weight of ceramide NS, 0.002% by weight of ceramide AS, 0.001% by weight of ceramide EOP, 0.001% by weight of ceramide AP, caprylic / capric 0.2% by weight of triglyceride, 0.2% by weight of baobab seed oil, 0.1% by weight of hydrogenated lecithin, 0.001% by weight of glycolipid, 0.006% by weight of tocopherol, 0.001% by weight of phytosterol, and 0.001% by weight of sodium hyaluronate.

In one embodiment, the weight ratio of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP is 100: 2: 2: 1: 1, and the weight ratio of hydrogenated lecithin and glycolipid is 100: 1, The weight ratio of tocopherol, phytosterol and sodium hyaluronate may be 6:1:1.

In one embodiment, the liposome is composed of two inner and outer phospholipid bilayer structures based on the center, and the inner phospholipid bilayer is composed of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP It is composed of five kinds of ceramides, baobab seed oil, and hydrogenated lecithin, and the outer phospholipid double layer may be composed of tocopherol, glycolipid, and phytosterol.

In one embodiment, in the step of obtaining the tertiary mixture, continuously stirring twice under a high-pressure condition of 1,000 to 1,500 bar and a temperature condition of 80 ° C using a high-pressure homomixer at a rotational speed of 6,000 rpm for 25 minutes After stirring with (first stirring), it may be stirring at a rotational speed of 4,500 rpm for 10 minutes (secondary stirring).

The cosmetic composition containing liposomes prepared according to the production method of the present invention has the advantage of having a small particle size and increased dissolving power and dispersing power while being able to more stably collect active substances.

In addition, the cosmetic composition containing the liposome according to the present invention is easily absorbed into the skin, has a soft feeling, strengthens the skin barrier, strengthens the oil-moisture balance, improves skin protection and antioxidant effects, and simultaneously achieves excellent formulation stability. There are advantages that can be

1 is a schematic diagram showing the structure of a liposome prepared according to the present invention. As shown in Figure 1, the liposome according to the present invention has an inner and outer phospholipid bilayer structure.
2 is a schematic diagram showing a process of obtaining a tertiary mixture using a high-pressure homomixer according to the manufacturing method of the present invention.

In the following, preferred embodiments of the present invention will be described in detail.

However, specific structural or functional descriptions of the embodiments according to the concept of the present invention disclosed in this specification are only exemplified for the purpose of explaining the embodiments according to the concept of the present invention, and the embodiments according to the concept of the present invention may be embodied in various forms and are not limited to the embodiments described herein.

In addition, although terms such as first, second, and third are used to describe various elements in various embodiments of the present specification, these elements should not be limited by these terms. These terms are only used to distinguish one component from another. Thus, what is referred to as a first element in one embodiment may be referred to as a second element in another embodiment. Each embodiment described and illustrated herein also includes its complementary embodiments. In addition, in this specification, 'and/or' is used to mean including at least one of the elements listed before and after.

In the specification, expressions in the singular number include plural expressions unless the context clearly dictates otherwise. In addition, the terms "comprise" or "having" are intended to designate that the features, numbers, steps, components, or combinations thereof described in the specification exist, but one or more other features, numbers, steps, or components. It should not be construed as excluding the possibility of the presence or addition of elements or combinations thereof.

In the present invention, five types of ceramides consisting of ceramide NP, ceramide NS, ceramide AS, ceramide EOP, and ceramide AP are mixed, glycerin and 1,2-hexanediol are added, and then caprylic/capric triglyceride and baobab are mixed. Adding seed oil and dissolving under a temperature condition of 80 ° C. to obtain a primary mixture;

adding hydrogenated lecithin and glycolipid to the first mixture and stirring at a rotational speed of 3,000 rpm for 1 hour under a temperature condition of 80° C. using a homomixer to obtain a second mixture;

Purified water, tocopherol, phytosterol, and sodium hyaluronate were added to the secondary mixture, and agitated twice continuously under high-pressure conditions of 1,000 to 1,500 bar and temperature conditions of 80 ° C. using a high-pressure homomixer to form liposomes. obtaining a tea mixture; and

filtering the tertiary mixture through a filter having a size of 0.45 μm to obtain liposomes having a size of less than 0.45 μm formed in the tertiary mixture;

It provides a method for producing a liposome-containing cosmetic composition comprising a.

Specifically, the manufacturing method according to the present invention is characterized in that all five types of ceramides consisting of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP are mixed and used.

Ceramide is a type of sphingolipids in which sphingosine and fatty acids are linked by an amide bond. It exists in the skin barrier, that is, the stratum corneum, and is a substance constituting intercellular lipids that plays an important role in skin moisturizing. Ceramide NP, Ceramide NS, Ceramide NH There are various types such as ceramide AP, ceramide AS, ceramide EOP and ceramide EOS.

In the present invention, among various types of ceramides, in particular, ceramide NP, ceramide NS, ceramide AS, ceramide EOP, and ceramide AP are selected and mixed in an appropriate amount, and a ceramide mixture is used in the manufacturing method.

As suggested in the present invention, when ceramide NP, ceramide NS, ceramide AS, ceramide EOP, and ceramide AP are mixed and used in appropriate amounts, the skin barrier is strengthened and the lipid protective film of the lamella layer of the skin is formed. It has a more effective filling function, can protect the skin by blocking moisture evaporation of the skin, and has the advantage of recovering skin damage caused by external causes.

In the present invention, ceramide NP may be included in an amount of 0.0001 to 10% by weight, preferably 0.0001 to 8% by weight, more preferably 0.001 to 5% by weight, and most preferably 0.01 to 3% by weight, based on the total weight of the cosmetic composition. can be included as

In addition, ceramide NS may be included in an amount of 0.00001 to 1% by weight based on the total weight of the cosmetic composition, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, Most preferably, it may be included in 0.0001 to 0.005% by weight.

In addition, ceramide AS may be included in an amount of 0.00001 to 1% by weight, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, based on the total weight of the cosmetic composition. Most preferably, it may be included in 0.0001 to 0.005% by weight.

In addition, ceramide EOP may be included in an amount of 0.00001 to 1% by weight based on the total weight of the cosmetic composition, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, and most preferably 0.0001 to 0.001% by weight. can be included

In addition, ceramide AP may be included in an amount of 0.00001 to 1% by weight based on the total weight of the cosmetic composition, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, and most preferably 0.0001 to 0.001% by weight. can be included

As one specific example according to the present invention, the manufacturing method according to the present invention is based on the total weight of the cosmetic composition, ceramide NP 0.1% by weight, ceramide NS 0.002% by weight, ceramide AS 0.002% by weight, ceramide EOP 0.001% by weight, ceramide AP 0.001 It can be prepared by adjusting the content to satisfy the weight %.

As one specific example according to the present invention, the manufacturing method according to the present invention can be prepared so that the weight ratio of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP is 100: 2: 2: 1: 1.

In this way, there is an advantage of satisfying the skin improvement effect and formulation stability at the same time as the contents of the five ceramides are satisfied. Specifically, when the content of ceramide is less than the above range, the effect of improving skin conditions such as skin barrier, skin moisturizing, elasticity, wrinkle improvement, etc. cannot be sufficiently exerted, and when it exceeds the above range, formulation stability is reduced and ceramide is precipitated problems can arise.

That is, by satisfying the content or weight ratio of the five ceramide components presented in the present invention, there is an advantage in that a stable formulation can be maintained when dispersed in an aqueous solution while achieving an excellent skin improvement effect.

Next, after mixing 5 types of ceramides as described above, after adding glycerin and 1,2-hexanediol, caprylic / capric triglyceride and baobab seed oil were added to a temperature condition of 80 ° C. A primary mixture can be obtained by dissolving under

Baobab seed oil is an oil extracted from the seeds of the baobab tree. In particular, the oil component extracted from the seeds not only improves damaged skin elasticity but is also rich in vitamins A, D, E and F. In the present invention, baobab seed oil has an antioxidant effect, prevents skin aging, and has an effect of softening the skin by improving skin texture through excellent moisturizing action.

As one embodiment according to the present invention, the manufacturing method according to the present invention is prepared by adjusting the content to satisfy 0.2% by weight of caprylic / capric triglyceride and 0.2% by weight of baobab seed oil, based on the total weight of the cosmetic composition. can

Next, hydrogenated lecithin and glycolipids are added to the primary mixture obtained by the above method, and stirred at a rotational speed of 3,000 rpm for 1 hour under a temperature condition of 80° C. using a homomixer to obtain a secondary mixture.

Hydrogenated lecithin is a substance that regulates moisture in cells and can impart luster and luster to the skin. Hydrogenated lecithin in the present invention contains an unsaturated fatty acid that is easily oxidized, and has an effect of making the skin healthy through an antioxidant action that prevents oxidative destruction from harmful oxygen.

In addition, lecithin is a type of phospholipid and is one of the important components for forming cell membranes, helping to maintain normal cell homeostasis. Accordingly, there is an advantage in that it can help regenerate damaged cells or prevent abnormal proliferation to help activate cells.

Glycolipids are a type of lipids composed of sugars and fats, and form important components of cell walls and ceramides. In the present invention, glycolipids play a role in forming a wall that coats cell walls and retains moisture in the skin, and plays an important role in forming a stable micelle structure in the aqueous phase by being involved in polarity balance control.

In the present invention, the glycolipid may be included in an amount of 0.00001 to 1% by weight, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, and most preferably 0.0001 to 0.001% by weight, based on the total weight of the cosmetic composition. can be included as

As one specific example according to the present invention, the manufacturing method according to the present invention can be prepared by adjusting the contents to satisfy 0.1% by weight of hydrogenated lecithin and 0.001% by weight of glycolipids with respect to the total weight of the cosmetic composition.

As one specific example according to the present invention, the manufacturing method according to the present invention may be prepared so that the weight ratio of hydrogenated lecithin and glycolipid is 100:1.

When each component of hydrogenated lecithin and glycolipid satisfies the above content or weight ratio, a more stable formulation can be formed, and when the above content or weight ratio is not satisfied, the formulation stability decreases and the formed double layer structure is maintained. Difficult problems can arise.

Next, purified water, tocopherol, phytosterol, and sodium hyaluronate were added to the secondary mixture obtained by the above method, and a high-pressure homomixer was used to form liposomes under high-pressure conditions of 1,000 to 1,500 bar and temperature conditions of 80 ° C. Stir twice in succession to obtain a tertiary mixture.

Tocopherol is a type of vitamin E and plays a role in protecting cell membranes by removing active oxygen. Free radicals, also called free radicals, cause undesirable oxidation in the body and destroy structures such as cell membranes and DNA. In the present invention, tocopherol is a type of vitamin E, and may have an effect of preventing cell membrane damage to prevent skin aging, maintaining skin elasticity, and imparting a moisturizing effect.

Phytosterol is a generic term for sterols contained in plants, and plays an important role in the formation of a healthy protective film of stratum corneum cells. In the present invention, phytosterols protect the protective film of the stratum corneum, help normalize the oil-moisture balance control function, and have excellent effects in imparting elasticity to the skin and softening the skin texture. It also helps in the synthesis of skin collagen and hyaluronic acid, helping to firm up the skin structure.

Sodium hyaluronate is an ingredient that helps maintain skin structure, skin elasticity, and skin regeneration by having excellent water retention. In the present invention, sodium hyaluronate has the effect of making the skin elastic, preventing wrinkles, activating skin cells to help skin regeneration, and preventing inflammation to minimize skin trouble.

In the present invention, phytosterol may be included in an amount of 0.00001 to 1% by weight, preferably 0.00001 to 0.1% by weight, more preferably 0.0001 to 0.01% by weight, and most preferably 0.0001 to 0.001% by weight, based on the total weight of the cosmetic composition. can be included

As one embodiment according to the present invention, the manufacturing method according to the present invention can be prepared by adjusting the content to satisfy 0.006% by weight of tocopherol, 0.001% by weight of phytosterol, and 0.001% by weight of sodium hyaluronate, based on the total weight of the cosmetic composition. .

As one specific example according to the present invention, the weight ratio of tocopherol, phytosterol and sodium hyaluronate can be prepared to be 6: 1: 1.

When each component of tocopherol, phytosterol, and sodium hyaluronate satisfies the above content or weight ratio, there is an advantage in that skin improvement effect and body shape stability can be satisfied at the same time. Specifically, when the content of each component is less than the above range, the oil-moisture balance control function is not sufficient, so that the effect of improving skin conditions such as skin elasticity and skin regeneration cannot be sufficiently exerted. If the content exceeds the above range, formulation stability is reduced. It is difficult to maintain the double-layered structure formed by this, or problems such as precipitation of active ingredients may occur.

As one embodiment according to the present invention, the step of obtaining a tertiary mixture according to the present invention can be obtained by continuously stirring twice under a high pressure condition of 1,000 to 1,500 bar and a temperature condition of 80 ° C using a high pressure homomixer, At this time, stirring twice may be first stirring at a rotation speed of 6,000 rpm for 25 minutes (first stirring), then stirring at a rotation speed of 4,500 rpm for 10 minutes (second stirring).

As described above, by continuously stirring twice under a high-pressure condition of 1,000 to 1,500 bar and a temperature condition of 80° C. using a high-pressure homomixer, the yield of small-sized liposomes to be achieved in the present invention can be increased. At this time, although not necessarily limited, as suggested in the present invention, after stirring at a rotation speed of 6,000 rpm for 25 minutes (first stirring), stirring at a rotation speed of 4,500 rpm for 10 minutes (second stirring) stirring), more liposomes with a size of less than 0.45 μm, which is to be achieved in the present invention, could be obtained.

Next, the tertiary mixture obtained by the above method is filtered through a filter having a size of 0.45 μm to obtain liposomes having a size of less than 0.45 μm formed in the tertiary mixture.

Liposomes prepared according to the present invention are composed of two phospholipid bilayer structures, the inner and the outer, based on the center. At this time, the inner phospholipid double layer is composed of 5 types of ceramides composed of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP, baobab seed oil, and hydrogenated lecithin, and the outer phospholipid double layer is It is characterized by being composed of tocopherols, glycolipids and phytosterols (see FIG. 1).

In the present invention, by obtaining liposomes having a size of less than 0.45 μm having two phospholipid bilayer structures as described above, effective substances can be more stably collected and ceramide encapsulation having a small particle size with increased dissolving power and dispersing power. A liposome material can be obtained. In addition, when the liposome according to the present invention is included in the cosmetic composition, it can be easily absorbed into the skin, and the hyaluronic acid-based formula has the advantage of having a softer feeling of use and excellent stability.

On the other hand, the formulation of the cosmetic composition obtained according to the present invention can be prepared in a preferred form according to the method of use, for example, lotion, essence, lotion, cream, ampoule, pack, foundation, hair shampoo, hair rinse, body wash, It can be manufactured in various forms such as gel, spray, foam cleansing, and ointment.

The cosmetic composition may include ingredients commonly used in cosmetic compositions in addition to the composition as active ingredients, for example, stabilizers, solubilizers, vitamins, pigments, and conventional adjuvants such as perfumes. .

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are merely illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications can be made within the scope and spirit of the present invention, and it is natural that such changes and modifications fall within the scope of the appended claims. will be.

Example

Example 1

According to the composition of Table 1 below, a cosmetic composition containing the liposome according to the present invention was prepared.

ingredient Content (% by weight) glycerin 30.0 1,2-Hexanediol 2.0 Caprylic/Capric Triglyceride 0.2 baobab seed oil 0.2 Hydrogenated Lecithin 0.1 Ceramide NP 0.1 tocopherol 0.006 Ceramide NS 0.002 Ceramide AS 0.002 Ceramide EOP 0.001 Ceramide AP 0.001 phytosterols 0.001 glycolipids 0.001 sodium hyaluronate 0.001 Purified water balance total 100.0

Preparation of primary mixture

Ceramide NP, Ceramide NS, Ceramide AS, Ceramide EOP and Ceramide AP were mixed to prepare a ceramide mixture, then glycerin and 1,2-hexanediol were added, followed by caprylic/capric triglyceride and baobab seeds A first mixture was prepared by adding oil and dissolving under a temperature condition of 80°C.

Preparation of secondary mixtures

Hydrogenated lecithin and glycolipid were added to the first mixture, and stirred at a rotation speed of 3,000 rpm for 1 hour under a temperature condition of 80 ° C. using a homomixer to prepare a second mixture.

Preparation of Tertiary Mixtures

Purified water, tocopherol, phytosterol, and sodium hyaluronate were added to the secondary mixture, and agitated twice continuously under high-pressure conditions of 1,000 to 1,500 bar and temperature conditions of 80 ° C. using a high-pressure homomixer to form liposomes. A tea mixture was prepared.

Preparation of cosmetic composition

The tertiary mixture was filtered through a filter having a size of 0.45 μm to obtain liposomes having a size of less than 0.45 μm formed in the tertiary mixture to prepare a cosmetic composition.

Example 2

It was prepared in the same manner as in Example 1, but after stirring at a rotation speed of 6,000 rpm for 25 minutes in the step of obtaining a tertiary mixture (first stirring), stirring at a rotation speed of 4,500 rpm for 10 minutes (second stirring) ) to obtain a tertiary mixture.

Comparative Examples 1 to 3

It was prepared in the same manner as in Example 1, except that it was prepared with the component content (wt%) of Table 2 below.

ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Ceramide NP 0.2 0.1 0.1 Ceramide NS 0.002 0.002 0.002 Ceramide AS 0.002 0.002 0.002 Ceramide EOP 0.001 0.001 0.001 Ceramide AP 0.001 0.001 0.001 glycolipids 0.001 0.01 0.001 phytosterols 0.001 0.001 0.01

Comparative Example 4

It is prepared in the same manner as in Example 1, but after obtaining the first mixture, the steps for preparing the second and third mixtures are not separately added, and hydrogenated lecithin, glycolipid, purified water, tocopherol, phytosterol, and sodium hyaluronate are added at the same time, A mixture was obtained by stirring at a rotational speed of 3,000 rpm for 1 hour under a temperature condition of 80° C. using a homomixer, and then filtered through a filter having a size of 0.45 μm.

Experimental example

Formulation stability confirmation

The cosmetic composition according to Examples 1 and 2 and Comparative Examples 1 to 4 was stored at 25° C. for 3 months and the appearance was observed to determine the stability of the formulation.

period Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 day stability stability stability stability stability stability 1 month stability stability Instability Instability Instability Instability 3 months stability stability Instability Instability Instability Instability

As shown in the table above, the formulation seemed relatively stable in all compositions on the day of manufacture, but in the case of Examples 1 and 2 of the present invention, it was confirmed that the formulation stability was well maintained even after 3 months, whereas the content of the present invention In the case of Comparative Examples 1 to 3 prepared outside the range of the weight ratio and the ceramide was precipitated within 1 month, and the formulation was separated, confirming that the formulation stability was not excellent. In addition, in the case of Comparative Example 4, which is divided into secondary and tertiary mixture preparation steps as in the present invention and does not undergo stirring under high pressure conditions, the formulation is separated within one month and the formulation surface is unbalanced, resulting in excellent formulation stability. confirmed that it does not.

That is, in the case of Examples 1 and 2 prepared according to the production method of the present invention, it can be confirmed that the double layer structure of the liposome can be stably maintained for a long time than in the case of Comparative Examples 1 to 4, and thus the formulation stability is much superior. .

Wrinkle improvement effect evaluation

The wrinkle improvement effect of the cosmetic compositions according to Example 1 and Comparative Example 4 was evaluated.

10 women each in their 20s, 30s, and 40s were selected as targets, using the product of Example 1 on one side of the face and using the product of Comparative Example 4 on the other side of the face, after 8 weeks The wrinkle improvement effect was evaluated using an expert's visual evaluation.

division Great a little doesn't exist Effectiveness (%) Example 1 19 7 4 86.7 Comparative Example 4 4 12 16 53.4

As shown in the table above, Example 1 was evaluated to have a skin moisturizing effect with an effective rate of 86.7%, whereas Comparative Example 4 was evaluated to have an effective rate of 53.4%. That is, it can be confirmed that the cosmetic composition of Example 1, which can maintain the stabilized liposome structure prepared according to the present invention for a long time, has a significantly superior wrinkle improvement effect than that of Comparative Example 4.

feeling of use

Sensory evaluation of 30 women evaluated the feeling of use (moisture retention, spreadability). Twice a day (morning and evening), the cosmetic composition of each Example and Comparative Example was applied to the face in an appropriate amount, and after 1 minute, the feeling of use was evaluated using a 5-point scale. The results on the day of use, after 3 days, after 7 days, and after 14 days are shown in the table below.

evaluation date division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 day Moisturizing
persistence 4.8 4.9 4.0 4.1 4.0 4.4 spreadability 4.7 4.8 4.1 4.1 3.9 4.2 3 days later Moisturizing
persistence 4.5 4.7 3.6 3.9 3.9 4.0 spreadability 4.6 4.8 3.8 3.8 3.7 4.1 7 days later Moisturizing
persistence 4.3 4.5 3.5 3.7 3.5 3.8 spreadability 4.7 4.7 3.6 3.8 3.5 3.7 after 14 days Moisturizing
persistence 4.3 4.4 3.2 3.5 3.4 3.7 spreadability 4.5 4.6 3.3 3.5 3.4 3.6

(5: very good, 4: excellent, 3: average, 2: poor, 1: very poor)

As shown in the table above, while the cosmetic compositions of Examples 1 and 2 according to the present invention showed excellent moisturizing durability and spreadability, Comparative Examples 1 to 2 prepared with contents and weight ratios outside the scope of the present invention In the case of Comparative Example 4, which was prepared by proceeding at once without dividing the 3rd, 2nd mixture and 3rd mixture preparation steps, the effect was not superior to the compositions of Examples 1 and 2 in all items of feeling of use evaluated. confirmed what was shown.

Claims (5)

After mixing 5 types of ceramides consisting of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP, adding glycerin and 1,2-hexanediol, caprylic/capric triglyceride and baobab seed oil adding and dissolving under a temperature condition of 80° C. to obtain a primary mixture;
adding hydrogenated lecithin and glycolipid to the first mixture and stirring at a rotational speed of 3,000 rpm for 1 hour under a temperature condition of 80° C. using a homomixer to obtain a second mixture;
Purified water, tocopherol, phytosterol, and sodium hyaluronate were added to the secondary mixture, and agitated twice continuously under high-pressure conditions of 1,000 to 1,500 bar and temperature conditions of 80 ° C. using a high-pressure homomixer to form liposomes. obtaining a tea mixture; and
filtering the tertiary mixture through a filter having a size of 0.45 μm to obtain liposomes having a size of less than 0.45 μm formed in the tertiary mixture;
Method for producing a liposome-containing cosmetic composition comprising a. According to claim 1,
Based on the total weight of the cosmetic composition, ceramide NP 0.1% by weight, ceramide NS 0.002% by weight, ceramide AS 0.002% by weight, ceramide EOP 0.001% by weight, ceramide AP 0.001% by weight, caprylic / capric triglyceride 0.2% by weight, baobab 0.2% by weight of seed oil, 0.1% by weight of hydrogenated lecithin, 0.001% by weight of glycolipids, 0.006% by weight of tocopherols, 0.001% by weight of phytosterols, and 0.001% by weight of sodium hyaluronate. Method for producing a cosmetic composition containing liposomes. According to claim 1,
The weight ratio of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP was 100:2:2:1:1, the weight ratio of hydrogenated lecithin and glycolipid was 100:1, tocopherol, phytosterol and sodium hyaluronate. A method for producing a liposome-containing cosmetic composition, characterized in that the weight ratio of 6: 1: 1. According to claim 1,
The liposome is composed of two phospholipid bilayer structures, inner and outer, based on the center,
The inner phospholipid double layer is composed of 5 types of ceramides composed of ceramide NP, ceramide NS, ceramide AS, ceramide EOP and ceramide AP, baobab seed oil, and hydrogenated lecithin,
The method for producing a liposome-containing cosmetic composition, characterized in that the outer phospholipid double layer is composed of tocopherol, glycolipid and phytosterol. According to claim 1,
In the step of obtaining the tertiary mixture, stirring twice continuously under a high-pressure condition of 1,000 to 1,500 bar and a temperature condition of 80 ° C using a high-pressure homomixer is stirred at a rotational speed of 6,000 rpm for 25 minutes (1 Secondary stirring), stirring at a rotational speed of 4,500 rpm for 10 minutes (secondary stirring), characterized in that, a method for producing a liposome-containing cosmetic composition.

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