KR102343605B1 - 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 - Google Patents

비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 Download PDF

Info

Publication number
KR102343605B1
KR102343605B1 KR1020167035369A KR20167035369A KR102343605B1 KR 102343605 B1 KR102343605 B1 KR 102343605B1 KR 1020167035369 A KR1020167035369 A KR 1020167035369A KR 20167035369 A KR20167035369 A KR 20167035369A KR 102343605 B1 KR102343605 B1 KR 102343605B1
Authority
KR
South Korea
Prior art keywords
subsequence
sequence
target
blocker
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020167035369A
Other languages
English (en)
Korean (ko)
Other versions
KR20170003695A (ko
Inventor
데이비드 유 장
뤄지아 우
줴샤오 왕
Original Assignee
윌리엄 마쉬 라이스 유니버시티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 윌리엄 마쉬 라이스 유니버시티 filed Critical 윌리엄 마쉬 라이스 유니버시티
Publication of KR20170003695A publication Critical patent/KR20170003695A/ko
Application granted granted Critical
Publication of KR102343605B1 publication Critical patent/KR102343605B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020167035369A 2014-05-19 2015-05-19 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 Active KR102343605B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
US62/000,114 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Publications (2)

Publication Number Publication Date
KR20170003695A KR20170003695A (ko) 2017-01-09
KR102343605B1 true KR102343605B1 (ko) 2021-12-27

Family

ID=54554607

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020167035369A Active KR102343605B1 (ko) 2014-05-19 2015-05-19 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭

Country Status (13)

Country Link
US (2) US20170067090A1 (https=)
EP (3) EP4512908A3 (https=)
JP (4) JP2017515484A (https=)
KR (1) KR102343605B1 (https=)
CN (2) CN106661627B (https=)
AU (2) AU2015264357B2 (https=)
BR (1) BR112016027063A2 (https=)
CA (1) CA2985631A1 (https=)
DK (1) DK3146080T3 (https=)
ES (1) ES2861353T3 (https=)
PH (1) PH12016502542B1 (https=)
RU (1) RU2708992C2 (https=)
WO (1) WO2015179339A1 (https=)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en) 2013-12-05 2015-06-11 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
EP3082853A2 (en) 2013-12-20 2016-10-26 The Broad Institute, Inc. Combination therapy with neoantigen vaccine
US10993997B2 (en) 2014-12-19 2021-05-04 The Broad Institute, Inc. Methods for profiling the t cell repertoire
US10975442B2 (en) 2014-12-19 2021-04-13 Massachusetts Institute Of Technology Molecular biomarkers for cancer immunotherapy
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
WO2017117287A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Split-cycle and tape amplification
US11414686B2 (en) 2016-05-06 2022-08-16 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
WO2018140391A1 (en) * 2017-01-24 2018-08-02 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3697798A4 (en) * 2017-10-18 2021-08-11 Day Zero Diagnostics, Inc. SELECTIVE ENRICHMENT OF A POPULATION OF DNA IN A MIXED DNA SAMPLE BY TARGETED DELETION OF DNA AMPLIFICATION
US12331350B2 (en) * 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
CN108220444A (zh) * 2018-02-28 2018-06-29 无锡禾盛医疗器械有限公司 一种kras基因突变检测的引物探针组合及其应用
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
CA3122844A1 (en) * 2019-01-15 2020-07-23 Tangen Bioscience Inc. A method for suppressing non-specific amplification products in nucleic acid amplification technologies
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2020259678A1 (zh) * 2019-06-26 2020-12-30 南京金斯瑞生物科技有限公司 一种含有阻断物的寡核苷酸
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
WO2021125854A1 (ko) * 2019-12-20 2021-06-24 고려대학교 산학협력단 극소량의 희귀 단일 염기 변이체 검출용 프라이머 및 이를 이용하여 극소량의 희귀 단일 염기 변이체를 특이적이고 민감하게 검출하는 방법
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US20230220456A1 (en) * 2020-05-01 2023-07-13 William Marsh Rice University Quantitative blocker displacement amplification (qbda) sequencing for calibration-free and multiplexed variant allele frequency quantitation
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4172169A4 (en) * 2020-06-26 2025-04-30 William Marsh Rice University COMPREHENSIVE AMPLICON ENRICHEMENT
CN111647650B (zh) * 2020-07-06 2023-06-27 河南赛诺特生物技术有限公司 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US20230399687A1 (en) * 2020-11-02 2023-12-14 Nuprobe Usa, Inc. Quantitative Multiplex Amplicon Sequencing System
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN112522369A (zh) * 2020-12-08 2021-03-19 合肥欧创基因生物科技有限公司 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
WO2022146773A1 (en) 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
WO2022155397A1 (en) 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en) 2021-03-16 2022-09-22 Nuprobe Usa, Inc. High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) * 2021-11-01 2023-05-04 Nuprobe Usa, Inc. Rna quantitative amplicon sequencing for gene expression quantitation
CN120624641A (zh) * 2025-02-20 2025-09-12 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090053720A1 (en) * 2007-08-08 2009-02-26 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' nuclease activity

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
WO2007106534A2 (en) * 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
EP2207896B1 (en) * 2007-10-04 2014-01-29 Commonwealth Scientific and Industrial Research Organisation Nucleic acid amplification
ES2527653T3 (es) * 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
EP3249053A1 (en) * 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
KR101312241B1 (ko) * 2010-04-27 2013-09-27 사회복지법인 삼성생명공익재단 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
WO2011146403A2 (en) * 2010-05-16 2011-11-24 Reniguard Life Sciences, Inc. Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use
GB2506760B (en) * 2011-01-14 2015-07-22 Genefirst Ltd Allele specific primers for EGFR exon 21 specific mutations
EP2705162B1 (en) * 2011-05-04 2018-07-11 Biocept, Inc. Methods for detecting nucleic acid sequence variants
EP2798078B1 (en) * 2011-12-30 2018-02-14 Polowang Biotech Inc. Method and primer set for detecting mutation
EP2841598B1 (en) * 2012-04-25 2018-03-14 Ho, Tho Huu Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090053720A1 (en) * 2007-08-08 2009-02-26 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' nuclease activity

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Front Zool.,doi: 10.1186/1742-9994-5-12(2008.7.20.)
Japanese Journal of Applied Physics.,45(1B):509-512(2006.)

Also Published As

Publication number Publication date
JP2021019607A (ja) 2021-02-18
EP3901278A1 (en) 2021-10-27
US20170067090A1 (en) 2017-03-09
EP3146080A1 (en) 2017-03-29
EP3146080B1 (en) 2021-01-06
EP3901278B1 (en) 2024-10-23
EP4512908A2 (en) 2025-02-26
AU2015264357B2 (en) 2021-08-12
JP7126718B2 (ja) 2022-08-29
DK3146080T3 (en) 2021-04-12
AU2021266291C1 (en) 2024-12-12
PH12016502542A1 (en) 2017-04-10
AU2015264357A1 (en) 2017-01-05
ES2861353T3 (es) 2021-10-06
CN106661627A (zh) 2017-05-10
EP3146080A4 (en) 2017-11-01
US20220090168A1 (en) 2022-03-24
EP4512908A3 (en) 2025-03-26
CN114032234A (zh) 2022-02-11
RU2708992C2 (ru) 2019-12-12
RU2016149307A3 (https=) 2018-12-10
PH12016502542B1 (en) 2024-01-17
BR112016027063A2 (pt) 2017-10-31
CA2985631A1 (en) 2015-11-26
RU2016149307A (ru) 2018-06-20
AU2021266291A1 (en) 2021-12-09
JP2024167406A (ja) 2024-12-03
CN106661627B (zh) 2021-11-23
WO2015179339A1 (en) 2015-11-26
JP2022166131A (ja) 2022-11-01
KR20170003695A (ko) 2017-01-09
AU2021266291B2 (en) 2024-09-19
JP2017515484A (ja) 2017-06-15

Similar Documents

Publication Publication Date Title
KR102343605B1 (ko) 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
CA2894635C (en) Methods and primer sets for high throughput pcr sequencing
US7618773B2 (en) Headloop DNA amplification
EP3377652B1 (en) A method and a kit for nucleic acid detection
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
CN111808977A (zh) 一种由snp所引起利福平类抗生素耐药的抗性基因的特异性引物的设计方法及检测方法
HK1236578B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
EP3377651B1 (en) A method and a kit for nucleic acid detection
AU2003212087B2 (en) Headloop DNA amplification

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20161216

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20200513

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20210305

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20210927

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20211222

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20211223

End annual number: 3

Start annual number: 1

PG1601 Publication of registration