KR102324117B1 - 가닥 침입에 기초한 증폭에 의한 핵산 검출 - Google Patents

가닥 침입에 기초한 증폭에 의한 핵산 검출 Download PDF

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KR102324117B1
KR102324117B1 KR1020167016721A KR20167016721A KR102324117B1 KR 102324117 B1 KR102324117 B1 KR 102324117B1 KR 1020167016721 A KR1020167016721 A KR 1020167016721A KR 20167016721 A KR20167016721 A KR 20167016721A KR 102324117 B1 KR102324117 B1 KR 102324117B1
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rna
probe
seq
dna
nucleic acid
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KR20160085353A (ko
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케빈 에보이그보딘
미르코 브루머
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오리온 디아그노스티카 오와이
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/125—Modifications characterised by incorporating agents resulting in resistance to degradation
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1373—Displacement by a nucleic acid
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    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020167016721A 2013-11-22 2014-11-21 가닥 침입에 기초한 증폭에 의한 핵산 검출 Active KR102324117B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13194118.9 2013-11-22
EP13194118 2013-11-22
PCT/EP2014/075321 WO2015075198A1 (en) 2013-11-22 2014-11-21 Detection of nucleic acids by strand invasion based amplification

Publications (2)

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KR20160085353A KR20160085353A (ko) 2016-07-15
KR102324117B1 true KR102324117B1 (ko) 2021-11-10

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US (1) US11008625B2 (enExample)
EP (1) EP3071707B1 (enExample)
JP (1) JP6499656B2 (enExample)
KR (1) KR102324117B1 (enExample)
CN (1) CN106414764B (enExample)
AU (1) AU2014351800B2 (enExample)
CA (1) CA2930786C (enExample)
ES (1) ES2703792T3 (enExample)
RU (1) RU2694976C1 (enExample)
WO (1) WO2015075198A1 (enExample)

Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
GB201618035D0 (en) 2016-10-25 2016-12-07 Orion Diagnostica Oy Method
EP3601608A1 (en) * 2017-03-30 2020-02-05 Life Technologies Corporation Quantification of ngs dna by adapter sequence
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
EP3530755B1 (de) * 2018-02-26 2020-08-26 AGCT GmbH Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung
GB201908481D0 (en) * 2019-06-13 2019-07-31 Kit Science & Service Ltd In-situ concatenation of oligo-nucleotide probes for target detection
CN111690720B (zh) * 2020-06-16 2021-06-15 山东舜丰生物科技有限公司 利用修饰的单链核酸进行靶核酸检测的方法
GB202018125D0 (en) 2020-11-18 2020-12-30 Aidian Oy Method
WO2025205849A1 (ja) * 2024-03-27 2025-10-02 株式会社カネカ 標的核酸配列検出用の組成物又はキット

Citations (2)

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US20120219945A1 (en) 2011-02-24 2012-08-30 Samsung Techwin Co., Ltd. Use of single-stranded binding protein in amplifying target nucleic acid

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US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
GB9715522D0 (en) 1997-07-24 1997-10-01 Zeneca Ltd Assays
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KR100612551B1 (ko) * 1999-11-08 2006-08-11 에이켄 카가꾸 가부시끼가이샤 핵산의 합성방법
US20020061530A1 (en) 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
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WO1999021881A1 (en) 1997-10-27 1999-05-06 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US20120219945A1 (en) 2011-02-24 2012-08-30 Samsung Techwin Co., Ltd. Use of single-stranded binding protein in amplifying target nucleic acid

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CA2930786C (en) 2022-05-10
ES2703792T3 (es) 2019-03-12
AU2014351800B2 (en) 2021-04-08
RU2694976C1 (ru) 2019-07-18
JP2016537005A (ja) 2016-12-01
EP3071707B1 (en) 2018-10-31
CN106414764A (zh) 2017-02-15
WO2015075198A1 (en) 2015-05-28
US20160289747A1 (en) 2016-10-06
EP3071707A1 (en) 2016-09-28
AU2014351800A1 (en) 2016-06-09
CN106414764B (zh) 2020-10-30
RU2016116333A (ru) 2017-12-27
CA2930786A1 (en) 2015-05-28
JP6499656B2 (ja) 2019-04-10
US11008625B2 (en) 2021-05-18
KR20160085353A (ko) 2016-07-15

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