AU2014351800B2 - Detection of nucleic acids by strand invasion based amplification - Google Patents

Detection of nucleic acids by strand invasion based amplification Download PDF

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Publication number
AU2014351800B2
AU2014351800B2 AU2014351800A AU2014351800A AU2014351800B2 AU 2014351800 B2 AU2014351800 B2 AU 2014351800B2 AU 2014351800 A AU2014351800 A AU 2014351800A AU 2014351800 A AU2014351800 A AU 2014351800A AU 2014351800 B2 AU2014351800 B2 AU 2014351800B2
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rna
seq
dna
probe
sequence
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AU2014351800A1 (en
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Mirko BRUMMER
Kevin EBOIGBODIN
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Aidian Oy
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Orion Diagnostica Oy
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/125—Modifications characterised by incorporating agents resulting in resistance to degradation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1373—Displacement by a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2014351800A 2013-11-22 2014-11-21 Detection of nucleic acids by strand invasion based amplification Active AU2014351800B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13194118 2013-11-22
EP13194118.9 2013-11-22
PCT/EP2014/075321 WO2015075198A1 (en) 2013-11-22 2014-11-21 Detection of nucleic acids by strand invasion based amplification

Publications (2)

Publication Number Publication Date
AU2014351800A1 AU2014351800A1 (en) 2016-06-09
AU2014351800B2 true AU2014351800B2 (en) 2021-04-08

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AU2014351800A Active AU2014351800B2 (en) 2013-11-22 2014-11-21 Detection of nucleic acids by strand invasion based amplification

Country Status (10)

Country Link
US (1) US11008625B2 (enExample)
EP (1) EP3071707B1 (enExample)
JP (1) JP6499656B2 (enExample)
KR (1) KR102324117B1 (enExample)
CN (1) CN106414764B (enExample)
AU (1) AU2014351800B2 (enExample)
CA (1) CA2930786C (enExample)
ES (1) ES2703792T3 (enExample)
RU (1) RU2694976C1 (enExample)
WO (1) WO2015075198A1 (enExample)

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GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
GB201618035D0 (en) 2016-10-25 2016-12-07 Orion Diagnostica Oy Method
EP3601608A1 (en) * 2017-03-30 2020-02-05 Life Technologies Corporation Quantification of ngs dna by adapter sequence
CN120210336A (zh) * 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
EP3530755B1 (de) * 2018-02-26 2020-08-26 AGCT GmbH Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung
GB201908481D0 (en) * 2019-06-13 2019-07-31 Kit Science & Service Ltd In-situ concatenation of oligo-nucleotide probes for target detection
CN111690720B (zh) * 2020-06-16 2021-06-15 山东舜丰生物科技有限公司 利用修饰的单链核酸进行靶核酸检测的方法
GB202018125D0 (en) 2020-11-18 2020-12-30 Aidian Oy Method
WO2025205849A1 (ja) * 2024-03-27 2025-10-02 株式会社カネカ 標的核酸配列検出用の組成物又はキット

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WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
WO2009150467A1 (en) * 2008-06-11 2009-12-17 Geneform Technologies Limited Isothermal nucleic acid amplification
US20100055685A1 (en) * 2006-07-24 2010-03-04 Zygem Corporation Limited Isothermal detection methods and uses thereof
AU2011244916A1 (en) * 2007-06-21 2011-11-24 Gen-Probe Incorporated Instruments and method for exposing a receptacle to multiple thermal zones
US20120219945A1 (en) * 2011-02-24 2012-08-30 Samsung Techwin Co., Ltd. Use of single-stranded binding protein in amplifying target nucleic acid

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US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
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WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
US20100055685A1 (en) * 2006-07-24 2010-03-04 Zygem Corporation Limited Isothermal detection methods and uses thereof
AU2011244916A1 (en) * 2007-06-21 2011-11-24 Gen-Probe Incorporated Instruments and method for exposing a receptacle to multiple thermal zones
WO2009150467A1 (en) * 2008-06-11 2009-12-17 Geneform Technologies Limited Isothermal nucleic acid amplification
US20120219945A1 (en) * 2011-02-24 2012-08-30 Samsung Techwin Co., Ltd. Use of single-stranded binding protein in amplifying target nucleic acid

Also Published As

Publication number Publication date
CN106414764A (zh) 2017-02-15
US11008625B2 (en) 2021-05-18
WO2015075198A1 (en) 2015-05-28
CA2930786A1 (en) 2015-05-28
ES2703792T3 (es) 2019-03-12
CN106414764B (zh) 2020-10-30
RU2016116333A (ru) 2017-12-27
EP3071707B1 (en) 2018-10-31
AU2014351800A1 (en) 2016-06-09
RU2694976C1 (ru) 2019-07-18
CA2930786C (en) 2022-05-10
US20160289747A1 (en) 2016-10-06
JP2016537005A (ja) 2016-12-01
JP6499656B2 (ja) 2019-04-10
KR102324117B1 (ko) 2021-11-10
EP3071707A1 (en) 2016-09-28
KR20160085353A (ko) 2016-07-15

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