JP6499656B2 - 鎖侵入に基づく増幅による核酸の検出 - Google Patents
鎖侵入に基づく増幅による核酸の検出 Download PDFInfo
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- JP6499656B2 JP6499656B2 JP2016533209A JP2016533209A JP6499656B2 JP 6499656 B2 JP6499656 B2 JP 6499656B2 JP 2016533209 A JP2016533209 A JP 2016533209A JP 2016533209 A JP2016533209 A JP 2016533209A JP 6499656 B2 JP6499656 B2 JP 6499656B2
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/125—Modifications characterised by incorporating agents resulting in resistance to degradation
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
- C12Q2537/1373—Displacement by a nucleic acid
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- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13194118 | 2013-11-22 | ||
| EP13194118.9 | 2013-11-22 | ||
| PCT/EP2014/075321 WO2015075198A1 (en) | 2013-11-22 | 2014-11-21 | Detection of nucleic acids by strand invasion based amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016537005A JP2016537005A (ja) | 2016-12-01 |
| JP2016537005A5 JP2016537005A5 (enExample) | 2017-12-28 |
| JP6499656B2 true JP6499656B2 (ja) | 2019-04-10 |
Family
ID=49622734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016533209A Active JP6499656B2 (ja) | 2013-11-22 | 2014-11-21 | 鎖侵入に基づく増幅による核酸の検出 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11008625B2 (enExample) |
| EP (1) | EP3071707B1 (enExample) |
| JP (1) | JP6499656B2 (enExample) |
| KR (1) | KR102324117B1 (enExample) |
| CN (1) | CN106414764B (enExample) |
| AU (1) | AU2014351800B2 (enExample) |
| CA (1) | CA2930786C (enExample) |
| ES (1) | ES2703792T3 (enExample) |
| RU (1) | RU2694976C1 (enExample) |
| WO (1) | WO2015075198A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201410022D0 (en) | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
| GB201618035D0 (en) | 2016-10-25 | 2016-12-07 | Orion Diagnostica Oy | Method |
| US20210108253A1 (en) * | 2017-03-30 | 2021-04-15 | Life Technologies Corporation | Quantification of ngs dna by adapter sequence |
| EP4589017A3 (en) * | 2017-10-06 | 2025-10-08 | 10x Genomics, Inc. | Rna templated ligation |
| EP3530755B1 (de) * | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
| GB201908481D0 (en) * | 2019-06-13 | 2019-07-31 | Kit Science & Service Ltd | In-situ concatenation of oligo-nucleotide probes for target detection |
| CN111690720B (zh) * | 2020-06-16 | 2021-06-15 | 山东舜丰生物科技有限公司 | 利用修饰的单链核酸进行靶核酸检测的方法 |
| GB202018125D0 (en) | 2020-11-18 | 2020-12-30 | Aidian Oy | Method |
| WO2023129167A1 (en) * | 2021-12-30 | 2023-07-06 | Cue Health Inc. | Compositions and methods for isothermal amplification of nucleic acids |
| WO2025205849A1 (ja) * | 2024-03-27 | 2025-10-02 | 株式会社カネカ | 標的核酸配列検出用の組成物又はキット |
| WO2025205850A1 (ja) * | 2024-03-27 | 2025-10-02 | 株式会社カネカ | 核酸プローブ |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| ATE196322T1 (de) | 1995-05-05 | 2000-09-15 | Perkin Elmer Corp | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| GB9715522D0 (en) | 1997-07-24 | 1997-10-01 | Zeneca Ltd | Assays |
| US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
| US6441152B1 (en) | 1998-12-08 | 2002-08-27 | Boston Probes, Inc. | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
| WO2001034790A1 (en) * | 1999-11-08 | 2001-05-17 | Eiken Kagaku Kabushiki Kaisha | Method for synthesizing nucleic acid |
| US20020061530A1 (en) | 2000-07-31 | 2002-05-23 | Belotserkovskii Boris P. | Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation |
| US7468244B2 (en) * | 2001-09-27 | 2008-12-23 | University Of Delaware | Polymorphism detection and separation |
| US20040219565A1 (en) | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
| CA2512110A1 (en) * | 2002-12-31 | 2004-07-22 | Mmi Genomics, Inc. | Compositions, methods, and systems for inferring bovine breed |
| KR101041106B1 (ko) | 2003-11-25 | 2011-06-13 | 한면기 | 핵산과 단백질의 신규한 실시간 탐지방법 |
| US7824890B2 (en) * | 2005-02-19 | 2010-11-02 | Avacta Group Plc | Isothermal amplification of nucleic acids |
| JP5027126B2 (ja) | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ, インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
| NZ548731A (en) * | 2006-07-24 | 2008-12-24 | Zygem Corp Ltd | Isothermal detection methods and uses thereof |
| AU2011244916B2 (en) * | 2007-06-21 | 2013-07-18 | Gen-Probe Incorporated | Instruments and method for exposing a receptacle to multiple thermal zones |
| HRP20171286T1 (hr) * | 2008-06-11 | 2017-11-17 | Orion Pharma (Uk) Limited | Izotermna amplifikacija nukleinske kiseline |
| KR20120097214A (ko) | 2011-02-24 | 2012-09-03 | 삼성테크윈 주식회사 | 핵산 증폭 과정에 있어서 단일 가닥 결합 단백질의 용도 |
| US8809021B2 (en) * | 2011-04-07 | 2014-08-19 | Alere San Diego Inc. | Monitoring recombinase polymerase amplification mixtures |
| KR102146523B1 (ko) * | 2012-01-06 | 2020-08-20 | 조원창 | 표적 핵산의 향상된 증폭 |
| CN105358711A (zh) | 2013-04-25 | 2016-02-24 | 欧雷恩诊断公司 | 基于链侵入的dna扩增方法 |
| EP3058100A4 (en) * | 2013-10-18 | 2017-04-19 | California Institute of Technology | Enhanced nucleic acid identification and detection |
-
2014
- 2014-11-21 KR KR1020167016721A patent/KR102324117B1/ko active Active
- 2014-11-21 CA CA2930786A patent/CA2930786C/en active Active
- 2014-11-21 RU RU2016116333A patent/RU2694976C1/ru active
- 2014-11-21 WO PCT/EP2014/075321 patent/WO2015075198A1/en not_active Ceased
- 2014-11-21 JP JP2016533209A patent/JP6499656B2/ja active Active
- 2014-11-21 AU AU2014351800A patent/AU2014351800B2/en active Active
- 2014-11-21 CN CN201480073793.8A patent/CN106414764B/zh active Active
- 2014-11-21 EP EP14800105.0A patent/EP3071707B1/en active Active
- 2014-11-21 US US15/038,439 patent/US11008625B2/en active Active
- 2014-11-21 ES ES14800105T patent/ES2703792T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2694976C1 (ru) | 2019-07-18 |
| JP2016537005A (ja) | 2016-12-01 |
| WO2015075198A1 (en) | 2015-05-28 |
| CA2930786A1 (en) | 2015-05-28 |
| EP3071707A1 (en) | 2016-09-28 |
| CN106414764B (zh) | 2020-10-30 |
| CN106414764A (zh) | 2017-02-15 |
| RU2016116333A (ru) | 2017-12-27 |
| US11008625B2 (en) | 2021-05-18 |
| CA2930786C (en) | 2022-05-10 |
| EP3071707B1 (en) | 2018-10-31 |
| KR102324117B1 (ko) | 2021-11-10 |
| ES2703792T3 (es) | 2019-03-12 |
| KR20160085353A (ko) | 2016-07-15 |
| AU2014351800A1 (en) | 2016-06-09 |
| US20160289747A1 (en) | 2016-10-06 |
| AU2014351800B2 (en) | 2021-04-08 |
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