CN106414764B - 通过基于链侵入的扩增的核酸检测 - Google Patents

通过基于链侵入的扩增的核酸检测 Download PDF

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CN106414764B
CN106414764B CN201480073793.8A CN201480073793A CN106414764B CN 106414764 B CN106414764 B CN 106414764B CN 201480073793 A CN201480073793 A CN 201480073793A CN 106414764 B CN106414764 B CN 106414764B
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nucleic acid
nucleotides
seq
target nucleic
acid sequence
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CN106414764A (zh
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K·伊伯伊格柏丁
M·布鲁默
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Aidian Oy
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Orion Diagnostica Oy
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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CN201480073793.8A 2013-11-22 2014-11-21 通过基于链侵入的扩增的核酸检测 Active CN106414764B (zh)

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EP13194118 2013-11-22
EP13194118.9 2013-11-22
PCT/EP2014/075321 WO2015075198A1 (en) 2013-11-22 2014-11-21 Detection of nucleic acids by strand invasion based amplification

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EP (1) EP3071707B1 (enExample)
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KR (1) KR102324117B1 (enExample)
CN (1) CN106414764B (enExample)
AU (1) AU2014351800B2 (enExample)
CA (1) CA2930786C (enExample)
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Families Citing this family (9)

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GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
GB201618035D0 (en) 2016-10-25 2016-12-07 Orion Diagnostica Oy Method
EP3601608A1 (en) * 2017-03-30 2020-02-05 Life Technologies Corporation Quantification of ngs dna by adapter sequence
CN120210336A (zh) * 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
EP3530755B1 (de) * 2018-02-26 2020-08-26 AGCT GmbH Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung
GB201908481D0 (en) * 2019-06-13 2019-07-31 Kit Science & Service Ltd In-situ concatenation of oligo-nucleotide probes for target detection
CN111690720B (zh) * 2020-06-16 2021-06-15 山东舜丰生物科技有限公司 利用修饰的单链核酸进行靶核酸检测的方法
GB202018125D0 (en) 2020-11-18 2020-12-30 Aidian Oy Method
WO2025205849A1 (ja) * 2024-03-27 2025-10-02 株式会社カネカ 標的核酸配列検出用の組成物又はキット

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021881A1 (en) * 1997-10-27 1999-05-06 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
GB9715522D0 (en) 1997-07-24 1997-10-01 Zeneca Ltd Assays
US6441152B1 (en) * 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
KR100612551B1 (ko) * 1999-11-08 2006-08-11 에이켄 카가꾸 가부시끼가이샤 핵산의 합성방법
US20020061530A1 (en) 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
CA2512110A1 (en) * 2002-12-31 2004-07-22 Mmi Genomics, Inc. Compositions, methods, and systems for inferring bovine breed
KR101041106B1 (ko) 2003-11-25 2011-06-13 한면기 핵산과 단백질의 신규한 실시간 탐지방법
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
CA2616241C (en) * 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
NZ548731A (en) * 2006-07-24 2008-12-24 Zygem Corp Ltd Isothermal detection methods and uses thereof
AU2011244916B2 (en) * 2007-06-21 2013-07-18 Gen-Probe Incorporated Instruments and method for exposing a receptacle to multiple thermal zones
CN102119225B (zh) * 2008-06-11 2015-04-08 基因排列技术有限公司 等温核酸扩增
KR20120097214A (ko) * 2011-02-24 2012-09-03 삼성테크윈 주식회사 핵산 증폭 과정에 있어서 단일 가닥 결합 단백질의 용도
JP5961247B2 (ja) * 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
KR102146523B1 (ko) * 2012-01-06 2020-08-20 조원창 표적 핵산의 향상된 증폭
US10227660B2 (en) 2013-04-25 2019-03-12 Orion Diagnostica Oy Strand-invasion based DNA amplification method
US10196684B2 (en) * 2013-10-18 2019-02-05 California Institute Of Technology Enhanced nucleic acid identification and detection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021881A1 (en) * 1997-10-27 1999-05-06 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons

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CN106414764A (zh) 2017-02-15
US11008625B2 (en) 2021-05-18
WO2015075198A1 (en) 2015-05-28
CA2930786A1 (en) 2015-05-28
ES2703792T3 (es) 2019-03-12
RU2016116333A (ru) 2017-12-27
EP3071707B1 (en) 2018-10-31
AU2014351800A1 (en) 2016-06-09
RU2694976C1 (ru) 2019-07-18
CA2930786C (en) 2022-05-10
US20160289747A1 (en) 2016-10-06
JP2016537005A (ja) 2016-12-01
JP6499656B2 (ja) 2019-04-10
AU2014351800B2 (en) 2021-04-08
KR102324117B1 (ko) 2021-11-10
EP3071707A1 (en) 2016-09-28
KR20160085353A (ko) 2016-07-15

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