KR102285995B1 - A composition for burn or wound treatment or prevention comprising the extract of cynanchum atratum and phytosterol - Google Patents

Abstract

The present invention relates to a composition for preventing or treating a burn or wound, including an extract of Cynanchum atratum and phytosterol as active ingredients. The composition for preventing or treating a burn or wound according to the present invention, and a pharmaceutical composition and a cosmetic composition prepared therefrom advantageously have an effect of providing cell mobility and angiogenesis at a burn or wound site. In addition, the composition for preventing or treating a burn or wound according to an embodiment of the present invention shows an excellent effect of forming a wet environment and is advantageous in that the active ingredients of the composition can infiltrate rapidly into the wound by virtue of high flowability.

Description

A composition for preventing or treating burns or wounds comprising white rice extract and phytosterol as active ingredients

The present invention relates to a composition for preventing or treating burns or wounds, and more particularly, to a composition for preventing or treating burns or wounds containing white rice extract as an active ingredient.

The dermis plays a role in preventing the contraction of wounds and scarring. The dermis contains blood vessels, lymphatic vessels and nerves of the skin, and hair follicles, sweat glands and oil glands are also located deep in the dermis, which is mostly connective tissue. Collagen, the most common protein in the body, provides flexibility to the skin and supports its structure, mainly in the form of fibroblasts.

Fibroblasts are cells that form an important component of fibrous connective tissue, and active proliferation of fibroblasts can promote wound healing in burn wounds.

Burns are mainly caused by accidents, and can be classified into flame burns, scalding burns, electric current burns, chemical burns, ultraviolet burns, and contact burns depending on the cause.

A flame burn refers to a case of burns caused by fire or intense heat caused by a fire accident or explosion of propane gas or LPG gas. Hot water burns are burns caused by hot water, edible oil, steam, etc., and are mainly burns of 2nd degree or more, and are often worn by children. Electric current burns are burns caused by electric shock to the body, and can cause burns even at low voltages used in general households, and often cause serious aftereffects. Chemical burns are burns caused by contact with acids, alkalis, etc., or general organic solvents, and in some cases can cause serious disability. UV burns can be worn when exposed to strong sunlight in summer, and can be prevented to some extent by applying sunscreen to the skin. Contact burns are burns that occur when the skin is exposed to a hot iron plate, iron, electric blanket, or the like, and in most cases can progress to a third degree or higher burn.

In addition, the severity of burns is divided into first-degree, second-degree, third-degree, and fourth-degree burns according to the area and depth of the burn area, temperature and contact time of the object that caused the burn, and skin condition. can leave and require hospital treatment.

First-degree burns are accompanied by red, stinging, and pain in the skin. It causes damage to the epidermis, the outermost layer of the skin layer, and may swell with pain and redness. Symptoms go away within a few days, but there are cases where light dripping and pigmentation remain. After recovery, no scars (scars) remain. A sun burn is the most common example of a first degree burn.

Second-degree burns affect both the epidermis and dermis, causing erythema, pain, swelling, and blistering within 24 hours of the accident. These burns can also affect sweat glands or pores. Perceptually, there is a burning sensation and severe pain. When the blister bursts, it shows an erosive surface and a large amount of secretion is released. Particular attention is required when the burn area is more than 15% of the body surface area. It heals within a few weeks, but pigmentation or loss of pigmentation is often left in the spot. When secondary infection occurs, the local symptoms are more severe and the course lasts longer.

Third-degree burns affect the epidermis, dermis, and even the hypodermis, causing the skin to turn black or translucent white and clot the blood vessels just below the skin's surface. The burned area may become numb, but the patient feels excruciating pain, and the skin tissue and structures become necrotic, which takes a lot of time to heal and leaves behind scars. Two weeks after the accident, the scab peels off and the ulcer surface appears. There is a lot of secretion and it is easy to bleed, but gradually new tissue is formed and the epidermis is regenerated, leaving scars and healing. In the case of deep skin necrosis or secondary infection, healing is delayed and the scar surface becomes irregular, resulting in keloids or deformities or movement disorders. Particular attention is required when the burn area covers more than 10% of the body surface area.

A 4th degree burn refers to a case in which the tissues of the burned area have been carbonized and turned black. It occurs mainly in high-voltage electrical burns, and may occasionally occur when fungal infection occurs in deep second-degree burns. If the extent of the burn is more than 20%, a systemic body reaction may occur. Hypotension, shock, and acute renal failure may occur due to excessive fluid loss, and subsequent wound infection, pneumonia, sepsis, multiple organ dysfunction syndrome, etc. This may happen.

In the treatment of burns, it is important to heal as quickly as possible or to reduce the area of the burn. In the healing of burn wounds, the composition of a moist environment helps to regenerate the skin by promoting cell differentiation and migration by maintaining adequate moisture in the affected area. In addition, the initial treatment to prevent secondary infection by blocking the surface of the affected area and the external environment and to prevent conversion to deep burns is emphasized.

However, there has been no study reported that the white rice (Cynanchum atratum) extract has an effect on the healing of burns.

Republic of Korea Patent Publication No. 10-1374537

One embodiment of the present application provides a composition for the prevention or treatment of burns or wounds, characterized in that it contains Cynanchum atratum extract and Phytosterol as active ingredients in order to solve the conventional problems as described above. would like to provide

Another embodiment of the present application provides a manufacturing method for preparing the composition, the method comprising: pulverizing dried white rice to prepare dried white rice; using 80% (v/v) ethanol as an extraction solvent in the dried pulverized rice, stirring and extracting at room temperature for 1 to 3 hours, followed by filtration to prepare a white rice extract; concentrating the white rice extract with a reduced pressure concentrator to prepare an aqueous raw material; Preparing an oily raw material including phytosterol; After heating the prepared oil phase raw material and aqueous phase raw material at 75 to 85 ° C, respectively, adding the aqueous phase raw material to the oil phase raw material to prepare water-in-oil (w / o) fine emulsified particles; characterized in that it comprises a To provide a method for preparing a composition for preventing or treating burns or wounds.

Another embodiment of the present application, comprising the composition, balm (balm), dressing, ointment (ointment) and a pharmaceutical for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of patches A composition is provided.

Another embodiment of the present application includes the composition, and includes a solution, suspension, emulsion, paste, gel, cosmetic composition, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax It provides a cosmetic composition for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of foundation and spray.

The problems to be solved of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, one embodiment of the present application provides a composition for preventing or treating burns, characterized in that it includes an extract of Cynanchum atratum and Phytosterol as active ingredients.

In order to solve the above problems, one embodiment of the present application provides a composition for treating or preventing wounds, characterized in that it comprises an extract of Cynanchum atratum and Phytosterol as active ingredients.

In order to solve the above problems, one embodiment of the present application provides a composition for preventing or treating burns or wounds, characterized in that it comprises an extract of Cynanchum atratum and Phytosterol as active ingredients. .

In one embodiment of the present application, the phytosterol is selected from the group consisting of beta-sitosterol (β-sitosterol), campesterol, cholesterol (cholesterol), stigmasterol (stigmasterol) and stigmastanol (stigmastanol) It can be any one.

In one embodiment of the present application, the white rice extract is at least one selected from the group consisting of ethanol, methanol, butanol, hexane, ethyl acetate, butylene glycol, propylene glycol, dipropylene glycol and methylene chloride. It may be an extract obtained from white rice using an organic solvent containing

In one embodiment of the present application, the composition may have a ratio of white rice extract: phytosterol of 5: 1 to 1:1.

In one embodiment of the present application, the composition, 83.5 to 95% by weight of vegetable oil; 2 to 6% by weight of an emulsifier or dispersant; 2 to 10% by weight of beeswax; and 0.1 to 0.5% by weight of white rice extract.

In one embodiment of the present application, the vegetable oil may be any one or more selected from the group consisting of palm oil, olive oil, palm oil, canola oil, soybean oil and safflower extract oil.

In one embodiment of the present application, it may further include an epidermal growth factor.

One embodiment of the present application provides a manufacturing method for preparing a composition for preventing or treating burns or wounds, the method comprising: pulverizing dried white rice to prepare dried white rice; using 80% (v/v) ethanol as an extraction solvent in the dried pulverized rice, stirring and extracting at room temperature for 1 to 3 hours, followed by filtration to prepare a white rice extract; concentrating the white rice extract with a reduced pressure concentrator to prepare an aqueous raw material; Preparing an oily raw material including phytosterol; After heating the prepared oil phase raw material and aqueous phase raw material at 75 to 85 ° C, respectively, adding the aqueous phase raw material to the oil phase raw material to prepare water-in-oil (w / o) fine emulsified particles; characterized in that it comprises a To provide a method for preparing a composition for preventing or treating burns or wounds.

In one embodiment of the present application, in the step of preparing the microemulsion particles, the microemulsion particles may be selected from the group consisting of liposomes, niosomes, micelles, colloids and nanoparticles.

One embodiment of the present application, comprising the composition, for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of balm, dressing, ointment, and patch A pharmaceutical composition is provided.

One embodiment of the present application, including the composition, solution, suspension, emulsion, paste, gel, cosmetic composition, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, It provides a cosmetic composition for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of wax foundation and spray.

The composition for preventing or treating burns or wounds according to an embodiment of the present application has the advantage of having cell mobility and angiogenesis efficacy in the affected area due to burns or wounds.

The composition for preventing or treating burns or wounds according to an embodiment of the present application has an advantage in that it is excellent in the composition of a wet environment, and helps the active ingredient of the composition to quickly penetrate into the wound due to its high flowability.

The composition for preventing or treating burns or wounds according to an embodiment of the present application has an advantage that the active ingredient of the composition can be fixed to the wound for a long time due to its high viscosity and excellent fixing power.

Effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1A is an image of observing cell migration ability according to an embodiment of the present application for a scratch wound of keratinocytes (HaCaT).
1B is a graph showing the reduced wound area according to the treatment of an embodiment of the present application in a scratch wound of keratinocytes (HaCaT) as a wound healing ability.
2A is an image of observing cell migration ability according to Examples and Comparative Examples of the present application for scratch wounds of keratinocytes (HaCaT).
3A is an image of a tube generated for each sample treatment of vascular endothelial cells (HUVEC).
3b is a graph showing the number of tubes counted for each sample treatment of vascular endothelial cells (HUVEC) as a percentage compared to NC (negative control).
Figure 4a is an image of a wound on the 14th day in a burn-inducing rat model.
Figure 4b is a photograph of the affected tissue stained with H&E.

Hereinafter, the present application will be described in more detail.

The following specific structural or functional descriptions are only exemplified to describe embodiments according to the concept of the present application, and the embodiments according to the concept of the present application may be implemented in various forms, and It should not be construed as limiting.

Since the embodiment according to the concept of the present application may have various changes and may have various forms, specific embodiments will be described in detail herein. However, this is not intended to limit the embodiments according to the concept of the present application to a specific disclosed form, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present application.

The terms used herein are used only to describe specific embodiments and are not intended to limit the present application. The singular expression includes the plural expression unless the context clearly dictates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and are not to be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present specification. .

One embodiment of the present application provides a composition for preventing or treating burns or wounds, characterized in that it comprises an extract of Cynanchum atratum and Phytosterol as active ingredients.

As used herein, the term 'active ingredient' refers to an amount of a compound capable of promoting damaged skin regeneration, cell mobility effect, angiogenesis effect, and skin regeneration effect. The content of the extract included in the composition of the present application will vary depending on the formulation in which the composition is commercialized, the method in which the compound is applied to the skin, and the time it stays on the skin.

The white rice extract may be a solid content in a non-volatile state.

The term 'solid content' as used in the present application is made by concentrating powdered flour into a certain lump shape, and the solid white rice extract has the advantage of improving the wet strength of the composition to create a wet environment for the affected area with burn wounds. . In addition, it may further include a wet strength enhancer to provide a high wet environment.

In one embodiment of the present application, the phytosterol is selected from the group consisting of beta-sitosterol (β-sitosterol), campesterol, cholesterol (cholesterol), stigmasterol (stigmasterol) and stigmastanol (stigmastanol) It can be any one.

Specifically, the phytosterol may be beta-sitosterol.

In one embodiment of the present application, the white rice extract may include any one or more selected from the group consisting of ethanol, methanol, butanol, hexane, ethyl acetate, butylene glycol, propylene glycol, dipropylene glycol, and methylene chloride. can

Specifically, the white rice extract may be extracted using ultrasound or reflux extraction. When using the reflux extraction method, there is an advantage in that the production speed of the composition is shortened when the solid content is prepared by fast drying. More specifically, the white rice extract may use a reflux extraction method using ethanol.

In one embodiment of the present application, the composition may have a ratio of white rice extract: phytosterol of 5: 1 to 1:1.

The added ratio of the white rice extract: phytosterol may be 5:1 to 1:1, specifically, 3:1 to 1:1, and more specifically, 1.5:1 to 1:1. If the white rice extract is added higher than the addition ratio of phytosterol, there is a problem that cytotoxicity is confirmed and the exact cell migration ability cannot be confirmed. When the phosphorus phytosterol is stirred, there is a problem that the composition is not homogenized because it is not dispersed in w/o form.

In one embodiment of the present application, the composition, 83.5 to 95% by weight of vegetable oil; 2 to 6% by weight of an emulsifier or dispersant; 2 to 10% by weight of beeswax; and 0.1 to 0.5% by weight of white rice extract.

If the vegetable oil is less than 83.5 wt%, there is a problem in that the emulsifier or beeswax is stirred in a non-homogeneous state, and if it exceeds 95 wt%, there is a problem in emulsifying with the white rice extract.

The emulsifier or dispersant is, ceteth-3, cetes-2, cetes-1, ceteareth-2, polyethylene glycol, cetyl alcohol, cetearyl alcohol, glyceryl stearate, dicetyl phosphate and the group consisting of lecithin It may include any one or more selected from. In addition, if the emulsifier or dispersant is less than 2% by weight, there is a problem that the oil phase raw material and the aqueous phase raw material are not emulsified, and if it exceeds 6% by weight, it is not dispersed in w / o form and a non-homogenized composition is prepared. there is.

The beeswax is a solid animal wax containing palmitic acid ester and serotic acid of melicyl alcohol, does not cause allergies, and provides a protective film on the skin film of the affected area due to burns or cuts, It has the advantage of protecting the skin by preventing bacteria or impurities that can penetrate into the skin.

If the beeswax is less than 2% by weight, there is a problem that the moisturizing effect may be insignificant, and if it exceeds 10% by weight, there is a problem in that the cell migration effect of the composition is deteriorated.

If the content of the white rice extract is less than 0.1 wt%, there is a problem in that the therapeutic efficacy of wounds caused by burns is insignificant, and if it exceeds 0.5 wt%, the cytotoxicity of the composition is confirmed and the correct cell migration ability cannot be confirmed.

In one embodiment of the present application, the vegetable oil may be any one or more selected from the group consisting of palm oil, olive oil, palm oil, canola oil, soybean oil and safflower extract oil.

Specifically, the vegetable oil may be olive oil.

In one embodiment of the present application, epidermal growth factor (EGF) may be further included.

The epidermal growth factor has the advantage of having an excellent function in wound healing by promoting the proliferation and cell migration of keratinocytes in the affected area due to burns or wounds.

One embodiment of the present application provides a manufacturing method for preparing a composition for preventing or treating burns or wounds, the method comprising: pulverizing dried white rice to prepare dried white rice; using 80% (v/v) ethanol as an extraction solvent in the dried pulverized rice, stirring and extracting at room temperature for 1 to 3 hours, followed by filtration to prepare a white rice extract; concentrating the white rice extract with a reduced pressure concentrator to prepare an aqueous raw material; Preparing an oily raw material including phytosterol; After heating the prepared oil phase raw material and aqueous phase raw material at 75 to 85 ° C, respectively, adding the aqueous phase raw material to the oil phase raw material to prepare water-in-oil (w / o) fine emulsified particles; characterized in that it comprises a To provide a method for preparing a composition for preventing or treating burns or wounds.

In the step of preparing the white rice extract, if the stirring extraction time is less than 1 hour, there is a problem that the ground dried white rice is not completely extracted. If the stirring extraction time exceeds 3 hours, the crushed dried white rice is not homogeneous. manufactured, there is a problem that there is difficulty in filtration.

In addition, the stirring may be performed using a rotor/stator homogenizer such as a stirring vessel and a pressure homogenizer. These facilities provide high shear stress and shear rate, so that in the stage of preparing an oily raw material, a homogeneously dispersed solvent can be prepared even under a condition of high phytosterol concentration.

In addition to phytosterol, the oily raw material may further include vegetable oil, an emulsifier or dispersant, and beeswax.

In addition, when the prepared oily raw material and aqueous phase raw material are heated at 75 ° C. to 85 ° C., respectively, if it is less than 75 ° C, the oil phase raw material is not emulsified and the composition is coagulated. There is a problem in that the water-in-oil type (w/o) fine emulsified particles are not prepared in the step.

As used herein, the term “water-in-oil type” is a generic term for a form in which water is dispersed in the form of fine particles in oil, which is a dispersion medium, and indicates the dispersion state of the fine emulsified particles.

In the step of preparing the microemulsified particles, the microemulsified particles may be selected from the group consisting of liposomes, niosomes, micelles, colloids and nanoparticles.

The colloid may be in a hydrophobic colloidal state, and may be a gelatinous colloid in unstable equilibrium with the solvent it contains.

The fine emulsified particles may further include a dispersing agent. A surfactant is used as the dispersant, and it is particularly preferred in the present application that it has excellent emulsification, dispersion and penetration effects, is extremely stable in acid and alkali solutions, exhibits excellent interfacial properties even in aqueous solutions in which acids and alkalis coexist, and is uniform in liquid phase. As such, it may be nonionic.

In addition, the penetrating agent included in the dispersion solution of the present application is a kind of surfactant to facilitate the penetration of colloidal particles. In particular, it has strong wet permeability and thus helps the active ingredient of the composition to quickly penetrate into the wound.

One embodiment of the present application, comprising the composition, for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of balm, dressing, ointment, and patch A pharmaceutical composition is provided.

The pharmaceutical composition may be prepared using a diluent or excipient such as a commonly used filler, extender, binder, wetting agent, disintegrant, and surfactant. The pharmaceutical composition of the present application may be prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. In addition, in addition to the simple diluent water and liquid paraffin, various excipients, for example, a wetting agent, a sweetener, a fragrance, a preservative, etc. may be included. As the suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used.

One embodiment of the present application, including the composition, solution, suspension, emulsion, paste, gel, cosmetic composition, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, It provides a cosmetic composition for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of wax foundation and spray.

Additives added to the respective compositions are not limited, and general additives in the cosmetic field may be added. Examples of general additives in the cosmetic field include antibiotics, binders, disintegrants, diluents, lubricants, stabilizers, preservatives, fragrances, oil, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments and preservatives. at least one selected from

Hereinafter, the present invention will be described in detail by way of Examples, Comparative Examples and Experimental Examples. However, the following Examples and Comparative Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples.

[Example 1]

As a raw material for the aqueous phase, dried white rice was used and extracted with 80% ethanol to prepare 1.26% by weight of white rice extract. As an oily raw material, it was prepared by mixing 91.24 wt% of olive oil, 3.00 wt% of an emulsifier, 4.00 wt% of beeswax, and 0.50 wt% of beta-sitosterol. A composition for preventing or treating ointment-type burns or wounds containing water-in-oil (w/o) fine emulsified particles by heating each dissolved oily raw material and aqueous phase raw material at 75°C to 85°C, and then mixing the above raw materials was prepared.

[Example 2]

As a raw material for the aqueous phase, dried white rice was used and extracted with 80% ethanol to prepare 1.26% by weight of white rice extract. As an oily raw material, 91.24 wt% of olive oil, 5.00 wt% of an emulsifier, 5.00 wt% of beeswax, and 0.50 wt% of beta-sitosterol were prepared by mixing. A composition for preventing or treating chestnut-type burns or wounds containing water-in-oil (w/o) fine emulsified particles by heating each dissolved oily raw material and aqueous phase raw material at 75°C to 85°C, and then mixing the above raw materials was prepared.

[Example 3]

As a raw material for the aqueous phase, dried white rice was used and extracted with 80% ethanol to prepare 0.50 wt% of white rice extract. As an oily raw material, it was prepared by mixing 91.24 wt% of olive oil, 3.00 wt% of an emulsifier, 4.00 wt% of beeswax, and 0.50 wt% of beta-sitosterol. After heating each dissolved oily raw material and aqueous phase raw material at 75° C. to 85° C., the above raw materials were combined to prepare a composition for preventing or treating burns or wounds containing water-in-oil (w/o) fine emulsified particles. .

[Example 4]

As a raw material for the aqueous phase, dried white rice was used, and extracted with 80% ethanol to prepare 2.50% by weight of white rice extract. As an oily raw material, it was prepared by mixing 91.24 wt% of olive oil, 3.00 wt% of an emulsifier, 4.00 wt% of beeswax, and 0.50 wt% of beta-sitosterol. After heating each dissolved oily raw material and aqueous phase raw material at 75° C. to 85° C., the above raw materials were combined to prepare a composition for preventing or treating burns or wounds containing water-in-oil (w/o) fine emulsified particles. .

[Comparative Example 1]

As a raw material for the aqueous phase, 1.26 wt% of distilled water was prepared. As an oily raw material, it was prepared by mixing 91.24 wt% of olive oil, 3.00 wt% of an emulsifier, 4.00 wt% of beeswax, and 0.50 wt% of beta-sitosterol. A composition for preventing or treating ointment-type burns or wounds containing water-in-oil (w/o) fine emulsified particles by heating each dissolved oily raw material and aqueous phase raw material at 75°C to 85°C, and then mixing the above raw materials was prepared.

[Comparative Example 2]

As a raw material for the aqueous phase, dried white rice was used and extracted with 80% ethanol to prepare 1.26% by weight of white rice extract. As an oily raw material, 91.74 wt% of olive oil, 3.00 wt% of an emulsifier, and 4.00 wt% of beeswax were prepared by mixing. A composition for preventing or treating ointment-type burns or wounds containing water-in-oil (w/o) fine emulsified particles by heating each dissolved oily raw material and aqueous phase raw material at 75°C to 85°C, and then mixing the above raw materials was prepared.

[Experimental Example 1] Confirmation of cell mobility by mixing ratio (Cell migration)

In order to prepare the compositions for the prevention or treatment of burns or wounds of Examples 1 to 4 and confirm cell mobility by product mixing ratio, changes in cell mobility in Examples were observed for scratch wounds of keratinocytes (HaCaT).

The keratinocytes (HaCaT) used in the experiment ^{were inoculated with 2X10 5} in 24 plate wells, and then cultured for 24 hours in a culture medium composed of DMEM/HIGH GLUCOSE supplemented with 10% FBS and 1% penicillin/streptomycin. After washing with DMEM/HIGH GLUCOSE medium supplemented with 1% penicillin/streptomycin, a scratch was made in the center of the cells using 200 μL (yellow) TIP. Then, Examples 1 to 4 were treated at a concentration of 100 μg/mL, respectively, and photographed using a microscope at intervals of 0 h and 26 h.

The mixing ratios of Examples 1 to 4 are shown in Table 1 below, and the cell migration ability of Examples 1 and 3 to 4 is shown in FIG. A graph showing the wound healing ability as a percentage of the decreased wound area after 26 hours compared to the control at the area of 0 hours from sample treatment is shown in FIG. 1B .

Example 1 Example 2 Example 3 Example 4 olive oil 91.24% 88.24% 92% 90% emulsifier 3.00% 5.00% 3.00% 3.00% beeswax 4.00% 5.00% 4.00% 4.00% β-sitosterol 0.50% 0.50% 0.50% 0.50% white rice extract 1.26% 1.26% 0.50% 2.50% Sum(%) 100.00% 100.00% 100.00% 100.00%

As shown in Table 1, Examples 1 to 4 are the results of confirming cell mobility by preparing different compositions according to the amount of raw materials added for the composition for the prevention or treatment of burns or wounds, and Example 1 is 2 It is an ointment-type formulation that can be applied to wound wounds caused by primary and tertiary burns or scratches. It has high flowability and is excellent for creating a wet environment with high oil content. In addition, the high flowability helps the active ingredient to penetrate the wound quickly, and through this, the wound healing effect of the white rice extract and beta-sitosterol can be maximized. Example 2 is a balm type formulation having a higher viscosity than Example 1. It can be applied to primary burn wounds, and has a high viscosity, so it is easy to fix to the affected area and has good durability. Examples 3 and 4 were ointment-type formulations, and excellent cell mobility was confirmed compared to the control, but it can be seen that the efficacy of Example 4 was insignificant.

Therefore, Example 1 was selected as the composition for the prevention or treatment of burns or wounds of the most ideal mixing ratio.

[Experimental Example 2]

Cell mobility of Examples and Comparative Examples for scratch wounds of keratinocytes (HaCaT) in order to prepare a composition for preventing or treating burns or wounds of Example 1 and Comparative Examples 1 and 2 to confirm cell mobility of the product Changes were observed.

The keratinocytes (HaCaT) used in the experiment ^{were inoculated with 2X10 5} in 24 plate wells, and then cultured for 24 hours in a culture medium composed of DMEM/HIGH GLUCOSE supplemented with 10% FBS and 1% penicillin/streptomycin. After washing with DMEM/HIGH GLUCOSE medium supplemented with 1% penicillin/streptomycin, a scratch was made in the center of the cells using 200 μL (yellow) TIP. Thereafter, NC (negative control group), PC (positive control group), Examples 1 and Comparative Examples 1 and 2 were each treated at a concentration of 100 μg/mL and photographed using a microscope at intervals of 0h and 26h.

Cell migration ability was shown in Figure 2a by observing the scratch wound images at 0 hours and 26 hours after sample treatment under a microscope. The percentage values expressed in terms of the healing ability are shown in Table 2.

NC PC Example 1 Comparative Example 1 Comparative Example 2 wound healing ability - 17% 16% 7% 6%

As shown in Table 2, NC (negative control group), PC (positive control group), Examples 1 and Comparative Examples 1 and 2 were different depending on the amount of the raw material added to the composition for the prevention or treatment of burns or wounds. As a result of preparing the composition and confirming cell mobility, Example 1 was found to show a higher wound healing ability than Comparative Examples 1 and 2.

Therefore, it was found that the composition for the prevention or treatment of burns or wounds containing both the white rice extract and beta-sitosterol was more effective than the beta-sitosterol and the white rice extract alone.

[Experimental Example 3] Confirmation of in vitro blood vessel formation (Tube formation)

Angiogenesis ability was observed for each sample treatment of vascular endothelial cells (HUVEC) in order to confirm the angiogenesis ability of the product by preparing the composition for preventing or treating burns or wounds of Example 1 above.

In order to confirm that Example 1 can promote the epithelialization process among wound healing mechanisms by inducing angiogenesis, a tube formation assay was performed using Matrigel.

Specifically, 64 μL of matrigel was dispensed into 96 plate wells, respectively, and placed at room temperature for 10 minutes and 37° C. CO _{2 in an} incubator for 30 minutes or more. After dispensing into the wells coated with Matrigel so that the final treatment concentration of NC, PC and Example 1 was 50 μg/mL, it was _{placed again in a 37° C. CO 2} incubator for 30 minutes or more. Human Umbilical Vein Endothelial Cells (HUVEC) ^{were suspended in serum-free EBM-2 medium for seeding at 1.5 x 10 4} cells/well, aliquoted into Matrigel and sample-treated wells, followed by 37 for 10 hours. ℃ CO ₂ After culturing in an incubator, it was photographed under a microscope (40X).

An image of the generated tube is shown in FIG. 3A, and a graph showing the angiogenesis ability as a percentage compared to the NC by counting the number of generated tubes is shown in FIG. 3B, and the percentage value of the angiogenesis ability Table 3 shows.

NC PC Example 1 blood vessel
generative - 54% 53%

As shown in Table 3 and Figure 3a, NC (negative control group), PC (positive control group) and Example 1 were prepared for the composition for the prevention or treatment of burns or wounds, depending on the amount of the raw material added, different compositions were prepared As a result of confirming the angiogenesis ability, it was visually confirmed that active angiogenesis appeared in PC and Example 1 compared to NC.

In addition, as shown in Table 3 and FIG. 3b, it can be seen that the degree of blood vessel generation increases by 54% and 53% compared to NC in PC and Example 1, so that the angiogenesis efficacy in Example 1 among wound healing mechanisms I found this to be excellent.

[Experimental Example 4] In vivo burn wound healing effect

In the experiment, a total of 5 female SD (Sprague-Dawley) rats 7 to 8 weeks old and 250 to 300 g in weight were used. After shaving the back of the anesthetized rat, a circular steel bar with a diameter of 130 mm was heated in an alcohol lamp for 30 seconds and then brought into contact with the skin surface for 10 seconds to induce 3 second-degree burns per subject. The sample treatment groups were i) NC (normal saline); ii) PC, iii) Example 1. After the burn induced date was set to 0, 0.15 g of the sample was applied to the affected area twice daily from 0 to 14. At the time of the 14th day, a ring having the same hole as the induced image size was prepared and adhered around the affected area, and then the size and condition of the wound reduced compared to day 0 were observed and shown in FIG. 4A. In addition, the tissue of the affected area was collected on the 16th day and subjected to H&E staining, and was photographed with a microscope (40X, 100X) for histopathological observation, and is shown in FIG. 4b.

As shown in FIG. 4A , as a result of observation of burn wounds on the 14th day after induction of burns, it was confirmed that the wound size decreased in PC and Example 1 compared to NC. As a result of referring to FIG. 4b, in the affected area treated with PC and Example 1, the infiltration of neutrophils, macrophages, and lymphocytes was overall reduced compared to the NC treatment, and it was observed that blood vessels and fibroblasts were well formed compared to NC. In addition, it was observed that granulomas were well formed in the PC and Example 1 groups, confirming that Example 1 could promote wound healing in burn wounds.

Although the embodiments of the present application have been described above, the present application is not limited to the above embodiments, but may be manufactured in various different forms, and those of ordinary skill in the art to which this application belongs may or may not have access to the technical spirit of the present application. It will be understood that the invention may be embodied in other specific forms without changing essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (11)

A composition for preventing or treating burns or wounds, comprising an extract of white rice (Cynanchum atratum) and beta-sitosterol (β-sitosterol) as active ingredients.
delete The method according to claim 1,
The white rice extract,
It is characterized in that it is an extract obtained from white rice using an organic solvent containing at least one selected from the group consisting of ethanol, methanol, butanol, hexane, ethyl acetate, butylene glycol, propylene glycol, dipropylene glycol and methylene chloride. A composition for preventing or treating burns or wounds.
The method according to claim 1,
The composition is
A composition for preventing or treating burns or wounds, characterized in that the ratio of white rice extract: beta-sitosterol is 5: 1 to 1: 1.
The method according to claim 1,
The composition is
83.5 to 95% by weight of vegetable oil;
2 to 6% by weight of an emulsifier or dispersant;
2 to 10% by weight of beeswax; and
A composition for preventing or treating burns or wounds, comprising: 0.1 to 0.5% by weight of white rice extract.
6. The method of claim 5,
The vegetable oil is
A composition for preventing or treating burns or wounds, characterized in that at least one selected from the group consisting of palm oil, olive oil, palm oil, canola oil, soybean oil and safflower extract oil.
delete In the manufacturing method for preparing the composition of any one of claims 1 and 3 to 6,
pulverizing the dried white rice to prepare a dried white rice;
using 80% (v/v) ethanol as an extraction solvent in the ground dried white rice, stirring and extracting at room temperature for 1 hour to 3 hours, followed by filtration to prepare a white rice extract;
concentrating the white rice extract with a reduced pressure concentrator to prepare an aqueous raw material;
Preparing an oily raw material including beta-sitosterol;
After heating the prepared oily raw material and the aqueous phase raw material at 75 to 85 °C, respectively, adding the aqueous phase raw material to the oily raw material to prepare water-in-oil (w/o) fine emulsified particles;
Method for producing a composition for the prevention or treatment of burns or wounds, comprising a.
9. The method of claim 8,
In the step of preparing the fine emulsified particles,
The microemulsified particles are
A method for producing a composition for preventing or treating burns or wounds, characterized in that it is selected from the group consisting of liposomes, niosomes, micelles, colloids and nanoparticles.
Including the composition of any one of claims 1 and 3 to 6,
A pharmaceutical composition for the prevention or treatment of burns or wounds, characterized in that any one selected from the group consisting of balm, dressing, ointment and patch as a formulation of the composition.
delete

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