KR102221453B1 - An oral mucoadhesive film composition comprising a low molecular weight collagen peptide and a natural extract compound and an oral mucoadhesive film prepared using the same - Google Patents

Abstract

The present invention relates to an oral mucosa-attached film composition comprising a low-molecular collagen peptide and a natural extract complex, and an oral mucosa-attachable film prepared using the same, the composition of which is to prevent skin aging, improve wrinkles, strengthen skin elasticity, and whitening A low-molecular collagen peptide with a molecular weight of 1×10 ⁴ dalton or less that exhibits a function, an elastin peptide that has a function of increasing moisture retention, epidermal flexibility and improving skin elasticity, a water-soluble flurane having adhesiveness and film formation, and its original structure Denatured starch ㄱ of any one of acid-treated modified starch, hydroxypropylated modified starch, and α-modified starch with modified physical properties, glycerin that absorbs moisture in the air to give it a function as a moisturizing agent, and the tensile strength of the film A natural extract complex consisting of carrageenan, which masks bitter taste even when applied in the oral cavity, and vitamin tree fruit extract that has anti-inflammatory and antioxidant properties, blackberry leaf extract%, perilla leaf extract, and hydrophilicity And sodium polyacrylate exhibiting a hygroscopic function.

Description

An oral mucoadhesive film composition comprising a low molecular weight collagen peptide and a natural extract compound and an oral mucoadhesive film prepared using the same prepared using the same}

The present invention is to increase the absorption rate of the skin dermis and increase the absorption rate of the skin by producing a low-molecular collagen and various natural extract complex ingredients that are active in skin beauty such as skin aging prevention, wrinkle improvement, skin elasticity, and whitening in a film type attached to the oral mucosa. It relates to an oral mucosa-attached film composition comprising a low-molecular collagen peptide and a natural extract complex that can be applied in, and an oral mucosa-attached film prepared using the same.

Collagen is known to play a key role in creating and supporting various organs and body shapes in the human body, and recently, it is showing a lot of interest in the medical system such as plastic surgery and reconstructive surgery. Functional products such as fine wrinkles, blemishes, anti-aging, pigmentation, and skin regeneration are also being produced as cosmetics products. Collagen can be obtained from by-products such as skin, bones and tissues of animals or marine organisms, and contains essential amino acids such as glycine, proline, hydroxyl proline, and arginine.

However, collagen, which is a by-product of animals and marine organisms, is composed of proteins and most of them are of high molecular weight, so it cannot be absorbed through human skin, so it has an inherent problem that is not suitable for use in collagen foods or cosmetics.

Accordingly, the inventors of the present invention have studied a composition capable of maximizing the absorption power of collagen to the human body, and as a result, collagen absorption through the oral mucosa is significantly greater than the amount absorbed through normal skin. It was confirmed through experiments that it was effective in anti-aging, skin elasticity improvement, wrinkle improvement, skin aging prevention, whitening, etc., and the present invention was completed.

US Patent No. 08840935 (Orally administrable films and preparation thereof, 2014.09.23.) U.S. Patent No. 10272038 (Film dosage form with extended release mucoadhesive particles, April 30, 2019) Japanese Patent Laid-Open No. 2017-206531 (Transmucosal administration system for a pharmaceutical drug, 2017.11.24.)

The present invention was invented as a part of solving the problems of the conventional high molecular collagen, and the object of the present invention is to prevent skin aging, improve wrinkles, strengthen skin elasticity, whitening, etc. A low-molecular collagen peptide and natural extract complex that increases the absorption rate of the skin dermis and can be easily applied to the oral cavity by manufacturing a complex component in a film form attached to the oral mucosa. Also, a low-molecular collagen peptide is used as the main component to increase the absorption rate in the body. To provide an oral mucosa-attached film composition comprising a and an oral mucosa-attached film prepared using the same.

The configuration of the problem solving means of the present invention for achieving the above object,
^{Low-molecular weight collagen peptide 1.5-22.0% by weight with a molecular weight of 1×10 4} dalton or less, exhibiting skin aging prevention, wrinkle improvement, skin elasticity, and whitening functions, moisture retention, increased epidermal flexibility and skin elasticity 0.1-1.0% by weight of elastin peptide that exhibits improved function, 50.0-54.0% by weight of water-soluble pullulan with adhesive and film-forming properties, acid-treated modified starch with modified original structure or physical properties, and hide Modified starch of either oxypropylated modified starch or α-modified starch 10.0-13.0 wt%, Glycerin 10.0-13.0 wt%, which absorbs moisture in the air and gives it a function as a moisturizing agent, film 0.6-2.0% by weight of carrageenan contained to increase the tensile strength of, 6.2-13.5% by weight of aspartame that masks bitter taste even when applied in the oral cavity, 15.0-32.0% by weight of vitamin tree fruit extract that has anti-inflammatory and antioxidant effects , Blackberry leaf extract 65.0-75.0% by weight, natural extract complex consisting of 3.0-10.0% by weight of perilla leaf extract 1.0-1.5% by weight, sodium polyacrylate 0.1-0.5% by weight exhibiting hydrophilic and hygroscopic functions ; Consists of

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An oral mucosa-attached film composition comprising a low-molecular collagen peptide and a natural extract complex according to the present invention, and an oral mucosa-attached film prepared using the same,

First, ingredients that are active in skin beauty such as skin aging prevention, wrinkle improvement, skin elasticity, whitening, etc. can be manufactured in the form of an oral mucosa film and can be conveniently applied in the oral cavity.

Second, the absorption rate in the body can be increased by using a low-molecular collagen peptide with a molecular weight of 1×10 ^{4 dalton or less as a main component.}

Third, it works more significantly on effects such as skin beauty by containing a natural extract complex composed of vitamin tree fruit, blackberry leaf, and perilla leaf.

1 is a graph measuring the amount of absorption of high molecular collagen permeated to oral mucosa cells and low molecular collagen according to the present invention in the evaluation of low molecular weight collagen permeability using 3D oral mucosa cells.
Figure 2 is a photograph showing the permeability of the high molecular collagen and the low molecular collagen according to the present invention to the oral mucosa cells in the evaluation of the permeability of low molecular collagen using 3D oral mucosa cells with a fluorescent label.
3 is a graph evaluating the effect of inhibiting the Elastase activity according to the content of the oral mucosa-attached film according to the present invention.
4 is a graph evaluating the effect of inhibiting collagenase activity according to the content of the oral mucosa-attached film according to the present invention.
5 is a graph evaluating the activity of promoting Type-1 procollagen synthesis according to the content of the oral mucosa-attached film according to the present invention.
6 is a graph evaluating the antioxidant effect according to the content of the oral mucosa-attached film according to the present invention.
7 is a graph evaluating the whitening effect through the evaluation of the Tyrosinase inhibitory activity of the oral mucosa-attached film according to the present invention.
8 is a graph evaluating the effect of improving skin wrinkles of the oral mucosa-attached film according to the present invention.
9 is a graph evaluating the effect of improving skin elasticity of the oral mucosa-attached film according to the present invention.

Hereinafter, an oral mucosa-attached film composition comprising a low molecular weight collagen peptide and a natural extract complex according to a preferred embodiment of the present invention will be described in detail.

Oral mucosa-attached film composition comprising the low-molecular collagen peptide and natural extract complex of the present invention,

^{Low-molecular weight collagen peptide 1.5-22.0% by weight with a molecular weight of 1×10 4} dalton or less, exhibiting skin aging prevention, wrinkle improvement, skin elasticity, and whitening functions, moisture retention, increased epidermal flexibility and skin elasticity 0.1-1.0% by weight of elastin peptide that exhibits improved function, 50.0-54.0% by weight of water-soluble pullulan with adhesive and film-forming properties, acid-treated modified starch with modified original structure or physical properties, and hide Modified starch of either oxypropylated modified starch or α-modified starch 10.0-13.0 wt%, Glycerin 10.0-13.0 wt%, which absorbs moisture in the air and gives it a function as a moisturizing agent, film 0.6-2.0% by weight of carrageenan contained to increase the tensile strength of, 6.2-13.5% by weight of aspartame that masks bitter taste even when applied in the oral cavity, 15.0-32.0% by weight of vitamin tree fruit extract that has anti-inflammatory and antioxidant effects , Blackberry leaf extract 65.0-75.0% by weight, natural extract complex consisting of 3.0-10.0% by weight of perilla leaf extract 1.0-1.5% by weight, sodium polyacrylate 0.1-0.5% by weight exhibiting hydrophilic and hygroscopic functions ; Consists of

The low molecular weight collagen peptide is a component that exhibits excellent effects on beauty such as preventing skin aging, improving wrinkles, strengthening skin elasticity, and whitening. In the present invention, collagen peptides (such as light proteins or albuminoids) to facilitate absorption of the skin dermis ^{The molecular weight is reduced to 1×10 4} dalton or less by reducing the molecular weight by a mechanism by any one or more of physical treatment, chemical treatment, and biological treatment. For example, the cell wall may be crushed to make it low molecular weight, or it may be made low molecular weight through hydrolysis treatment or the like.

As a result, the collagen peptide having a low molecular weight is easily absorbed in the application area, so that the blood concentration increases rapidly, thereby achieving a desired skin cosmetic effect.

According to a preferred embodiment of the present invention, the low molecular weight collagen peptide is contained in an amount of 1.5 to 22.0% by weight based on the total weight of the composition. If the low molecular weight collagen peptide is contained in less than 1.5% by weight, the amount absorbed into the skin dermis is insignificant, so that the functionality of the collagen cannot be sufficiently exhibited, and if it is contained in more than 22.0% by weight, the oral mucosa cannot maintain a smooth balance with other ingredients. Even if it is applied within, it is difficult to achieve the desired skin beauty effect.

The elastin peptide is a component having excellent functions of retaining moisture, increasing epidermal flexibility and improving skin elasticity.According to a preferred embodiment of the present invention, the elastin peptide is contained in an amount of 0.1 to 1.0% by weight based on the total weight of the composition. If the elastin peptide is contained in an amount of less than 0.1% by weight, effects such as moisture retention, epidermal flexibility and skin elasticity improvement cannot be exerted, and if it is contained in excess of 1.0% by weight, the balance between the components constituting the composition is broken and the oral mucosa Functions that are valid within are not working smoothly.

The flurane is water-soluble, has strong adhesion, and has excellent film-forming properties. According to a preferred embodiment of the present invention, the fluran is contained in an amount of 50.0 to 54.0% by weight based on the total weight of the composition. If the flurane is contained in an amount of less than 50.0% by weight, processing into a film form is not easy, and if it is contained in an amount exceeding 54.0% by weight, it is uneconomical because it contains more than necessary amount.

The modified starch is a modified starch that has undergone various processing treatments to modify its original structure and physical properties.In the present invention, it is selected from modified starch consisting of acid-treated modified starch, hydroxypropylated modified starch, α-modified starch, and starch decomposition product. Any one or more can be used.

According to a preferred embodiment of the present invention, the modified starch is contained in an amount of 10.0 to 13.0% by weight based on the total weight of the composition. If the modified starch is contained in an amount of less than 10.0% by weight, it shows a spherical molecular structure in an aqueous solution, so that the tensile strength of the film may be weakened or the film may not be formed. It causes an agglomeration phenomenon inside the molecule, making it impossible to form a film.

The glycerin is contained in order to impart a function as a moisturizing agent by absorbing moisture in the air, and according to a preferred embodiment of the present invention, it is contained in an amount of 10.0 to 13.0% by weight based on the total weight of the composition. When the glycerin is contained in an amount of less than 10.0% by weight, it does not function as a moisturizing agent, and when it is contained in an amount exceeding 13.0% by weight, the adhesiveness and elongation on the film surface may be increased, resulting in a problem in use.

The carrageenan is a hot water extract of seaweeds belonging to red algae, and has a basic structure of galactans having galactose as a repeating unit, and all of the structural units are sulfuric acid ester type or anhydrous type. The carrageenan is contained to increase the tensile strength of the film, and according to a preferred embodiment of the present invention, it is contained in an amount of 0.6 to 2.0% by weight based on the total weight of the composition. When the carrageenan is contained in an amount of less than 0.6% by weight, sufficient tensile strength is not exhibited, and when it exceeds 2.0% by weight, the tensile strength is excessively increased, making it difficult to process or cut.

The sweetener is intended to shield the bitter taste so that there is no discomfort even when applied in the oral cavity.In the present invention, any one selected from vitamin C (vitamin C), aspartame, xylitol, and lemon flavor, or these It can be made of a combination of.

According to a preferred embodiment of the present invention, the sweetener is contained in an amount of 6.2 to 13.5% by weight based on the total weight of the composition. If the sweetener is contained in an amount of less than 6.2% by weight, the function of the sweetener is insignificant, and if it exceeds 13.5% by weight, the flavor of the sweetener becomes strong, which may cause discomfort.

The natural extract complex is composed of a vitamin tree fruit extract, a blackberry leaf extract and a perilla leaf extract, and according to a preferred embodiment of the present invention, the natural extract complex is a vitamin tree fruit extract 15.0 to 32.0% by weight, blackberry It may consist of 65.0 to 75.0% by weight of leaf extract and 3.0 to 10.0% by weight of perilla leaf extract.

The vitamin tree, which is an object for obtaining the vitamin tree fruit extract, contains a large amount of vitamins and beta-carotene, and according to a preferred embodiment of the present invention, the vitamin tree fruit extract is a natural extract complex. It may be contained in an amount of 15.0 to 32.0% by weight based on the weight. When the vitamin tree fruit extract exceeds 32.0% by weight, the desired functional effects such as anti-inflammatory and antioxidant activities are sufficiently exhibited, but an uneconomic problem occurs due to the addition of an amount more than necessary, and when the amount is less than 15.0% by weight, the added amount As it is insignificant, it is difficult to expect desired functional effects such as anti-inflammatory and antioxidant effects.

Blackberry, which is a subject for obtaining the blackberry leaf extract, improves liver, kidney, and bladder functions, and has a very high content of active ingredients that are beneficial for anticancer and antioxidant activities.

According to a preferred embodiment of the present invention, the blackberry leaf extract may be contained in an amount of 65.0 to 75.0% by weight based on the total weight of the natural extract complex. When the amount of the blackberry leaf extract exceeds 75.0% by weight, the functional action of the blackberry leaf extract becomes excellent, whereas the functional action of the remaining ingredients except for the blackberry leaf extract becomes relatively inferior, resulting in skin occurring through various routes. It targets only some of the factors causing the disease, and as a result, the efficiency of skin care decreases. If it is less than 65.0% by weight, the functional action of the blackberry leaf decreases, that is, the anti-inflammatory action and antioxidant action are insignificant.

Perilla lobules, which are subjects for obtaining the perilla lobules extract, are 1-year-old herbs belonging to the Lamiaceae family (Labiatae), and are known to be effective in anti-inflammatory, anti-allergic, anti-asthma, and antioxidant activities in modern medicine.

According to a preferred embodiment of the present invention, the perilla leaf extract may be contained in an amount of 3.0 to 10.0% by weight based on the total weight of the natural extract complex. If the perilla leaf extract exceeds 10.0% by weight, the herbal action of perilla leaf is sufficiently exhibited, but it is uneconomical because more than necessary amount is added, and if the perilla leaf extract is less than 3.0% by weight, the effect such as anti-inflammatory is insignificant. .

The sodium polyacrylate is a sodium salt of an acrylic acid polymer, exhibits high hydrophilicity and hygroscopic properties, and is also used as a film forming agent, skin conditioning agent, softener, absorbent, and the like.

According to a preferred embodiment of the present invention, the sodium polyacrylate is contained in an amount of 0.1 to 0.5% by weight based on the total weight of the composition. When the sodium polyacrylate is contained in an amount of less than 0.1% by weight, functions such as hydrophilicity and hygroscopicity are insignificant, and when it is contained in an amount exceeding 0.5% by weight, a change in physical properties (solidification) may be noticeable.

In a further embodiment of the present invention, the oral mucosa-attached film composition is a cosmetically acceptable cosmetic adjuvant, a pharmaceutically acceptable pharmaceutical adjuvant, a food pharmaceutically acceptable food adjuvant, a biologically active compound, an absorbent substance, and a personal Any one of a care compound, an active ingredient, a cosmetic adjuvant, or a combination thereof may be further included.

On the other hand, the oral mucosa-attached film composition of the present invention composed of the above-described components is manufactured in a film form through treatments such as molding and processing. At this time, the thickness of the prepared film is preferably 10-500㎛. This is because when the thickness of the oral mucosa-attached film is less than 10 μm, it is not easy to adhere to the oral mucosa, and on the contrary, when the thickness of the oral mucosa-attached film exceeds 500 μm, absorption into the body is not easily achieved. to be.

Hereinafter, the present invention will be described in more detail through Examples and Experimental Examples.

[Example] Oral mucosa adhesive film production

Low-molecular weight collagen peptide 10.5% by weight, elastin peptide 0.5% by weight, flulan 52.0% by weight, modified starch 12.5% by weight, glycerin 12.0% by weight %, carrageenan (carrageenan) 1.0% by weight, sweetener 10.0% by weight, vitamin tree fruit extract, blackberry leaf extract and perilla leaf extract 1.0% by weight of a natural extract composition consisting of a mixture of 0.5% by weight of sodium polyacrylate (sodium polyacrylate) Next, in the form of a film that can be attached to the oral mucosa, an oral mucosa-attached film according to the present invention was prepared, and a plurality of the prepared films were cut into a size of 2.5 cm in width and 2.5 cm in length for ease of experimentation.

[Experimental Example 1] Evaluation of low molecular weight collagen permeability using 3D oral mucosal cells

1. Measurement of the amount of collagen permeated to the oral mucosa cells

In order to measure the amount of collagen permeated to the oral mucosa cells, the BCA Assay method was used. Donor Solution (collagen diluted in PBS) was added to the cell culture insert of 3D oral mucosal cells (Epioral, MatTek), and the Receiver Solution was recovered after 1 hour and 2 hours, and the amount of permeated collagen was measured using the BCA Assay method. The amount of collagen was calculated from a standard curve calculated from a standard solution.

In Figure 1, the amount of collagen permeated to the oral mucosa cells was quantified and shown. Referring to Fig. 1 above, the experimental results are described, as the amount of collagen permeated to the 3D oral mucosa cells increases as time elapses, and the amount of low molecular collagen permeation is significantly higher than that of high molecular collagen. It can be seen that the absorption of low-molecular collagen occurs faster than that of high-molecular collagen in cells.

2. Confirmation of collagen permeability to oral mucosa cells

Fluorescently labeled collagen was used to check the collagen permeability to oral mucosal cells. Donor Solution (FITC-Collagen diluted in PBS) was added to the Cell Culture Insert of 3D oral mucosal cells (Epioral, MatTek), and after 1 hour, it was fixed using 4% PFA solution. Thereafter, frozen sections were prepared and 3D oral mucosal cells were observed using a fluorescence microscope.

Figure 2 shows a picture of the collagen permeability observed with a fluorescence microscope in the oral mucosal cells. Referring to FIG. 2, the experimental results are described as above, when the fluorescently-labeled collagen is treated for the same period of time, the high molecular collagen is mainly distributed in the upper part of the oral mucosa, whereas the low-molecular collagen is seen to be transmitted from the top to the bottom of the oral mucosa. , It can be seen that absorption of low-molecular collagen is easier.

[Experimental Example 2] Confirmation of wrinkle improvement effect of oral mucosa-attached film

1. Elastase activity inhibition ability evaluation

In order to evaluate the anti-wrinkle effect of the oral mucosa-attached film of the present invention, the ability to inhibit Elastase activity was evaluated. 220 µl of a sample diluted by concentration in 0.1M Tris-HCl buffer (pH 8.0) and 20 µl of 2 mg/ml N-succinyl-Ala-Ala-Ala-ρ-nitroanilide were added and reacted at 25° C. for 10 minutes. After adding 20 µl of 1µg/ml porcine pancreatic elastase (PPE) and reacting for 20 minutes at 25℃, the reaction was terminated on ice, and the absorbance was measured at 405nm using a microplate reader. It was expressed as the absorbance reduction rate of the and untreated groups.

Figure 3 shows the results of the Elastase activity inhibitory ability to evaluate the wrinkle improvement effect. As shown in Figure 3, it can be seen that the treatment of the oral mucosa-attached film is a concentration-dependent Elastase activity inhibitory ability is observed. From these results, it can be concluded that the oral mucosa-attached film comprising the low-molecular collagen peptide according to the present invention has a significant effect on wrinkle improvement.

2. Evaluation of inhibitory ability of collagenase activity

In order to evaluate the anti-wrinkle effect of the oral mucosa-attached film containing a low-molecular collagen peptide and a natural extract complex, the ability to inhibit collagenase activity was confirmed. After diluting the sample by concentration, it was measured using a Collagenase Activity Assay kit (Abcam). After adding 2 µl of sample diluted by concentration and 10 µl of Collagenase to 88 µl of assay buffer, 100 µl of Collagenase Reaction Mix was added to react, and absorbance was measured at 345 nm using a microplate reader. It was expressed as the absorbance reduction rate of the and untreated groups.

Figure 4 shows the results of the collagenase activity inhibitory ability to evaluate the wrinkle improvement effect of the oral mucosa-attached film of the present invention.

As shown in Fig. 4, the treatment of the oral mucosa-attached film was observed to have a concentration-dependent collagenase activity inhibitory ability, and in view of these results, it was concluded that the oral mucosa-attached film according to the present invention had a significant effect on wrinkle improvement. Can be derived.

3. Evaluation of Type-1 procollagen synthesis promotion activity

In order to evaluate the anti-wrinkle effect of the oral mucosa-attached film containing a low-molecular collagen peptide and a natural extract complex, the activity of promoting Type-1 procollagen synthesis was confirmed. HDF cells (Human Dermal Fibroblast cells) were cultured in DMEM cell culture solution containing 10% FBS at 37°C and 5% CO ₂ conditions, and then spread on a 96-well plate to become ^{1×10 4 cells per well, and then for 12 hours.} The cells were completely attached while culturing. Cells in each well were washed with serum-free DMEM culture, and then the samples were treated in each well to a final concentration of 62.5, 125, 250, 500, 1000, and 2000 µg/ml, followed by incubation for 48 hours. The culture medium was collected and the collagen content was measured using a Procollagen type 1 C-peptide assay kit (Takara Bio Inc.), and the collagen concentration was calculated from a standard curve calculated from the standard solution included in the kit.

Fig. 5 shows the results of evaluating Type-1 procollagen synthesis promoting activity for evaluating the wrinkle improvement effect of the oral mucosa-attached film of the present invention.

Referring to Figure 5 to explain the experimental results, the treatment of the oral mucosa-attached film was observed to promote concentration-dependent Type-1 procollagen synthesis, and this result is the oral mucosa-attached type containing the low-molecular collagen peptide according to the present invention. It can be concluded that the film has a significant effect on wrinkle improvement.

[Experimental Example 3] Antioxidant Effect of Oral Mucosa Attached Film

In order to evaluate the antioxidant effect of the oral mucosa-attached film containing a low molecular weight collagen peptide and a natural extract complex, the free radical scavenging ability of DPPH was confirmed. To 200 μl of 200 μM DPPH (1,1-diphenyl-2-picryl hydrazyl) dissolved in methanol, 50 μl of samples diluted with 125, 250, 500, 1000, and 2000 μg/ml were added and reacted at 37° C. for 30 minutes. Absorbance was measured at 405 nm using a microplate reader. The free radical scavenging ability of DPPH was expressed as the rate of decrease in absorbance of the sample-treated group and the non-treated group.

Figure 6 shows the free radical scavenging ability of DPPH for showing the antioxidant effect of the oral mucosa film-type film of the present invention.

Referring to Figure 6 to explain the experimental results, it was observed that the concentration-specific treatment of the oral mucosa film containing a low molecular weight peptide as a main component increases the free radical scavenging ability of DPPH, and in light of this result, the oral mucosa adhesion according to the present invention It can be concluded that the type film has a significant effect on antioxidants.

[Experimental Example 4] Whitening effect of oral mucosa-attached film

In order to evaluate the whitening effect of the oral mucosa-attached film containing a low molecular weight collagen peptide and a natural extract complex, the ability to inhibit tyrosinase activity was confirmed. 50 µl of a sample and 50 µl of 100 unit/ml Tyrosinase were added to 50 µl of 0.1M phosphate buffer (pH 6.5), and reacted at room temperature for 5 minutes. After adding 100 µl of 1mM L-DOPA and reacting for 30 minutes at 37°C, absorbance was measured at 475 nm using a microplate reader, and the tyrosinase inhibitory activity was expressed as the decrease in absorbance of the sample treated and untreated groups.

Figure 7 shows the ability to inhibit Tyrosinase activity for showing the whitening effect of the oral mucosa-attached film of the present invention.

Referring to Figure 7 to describe the experimental results, the treatment of the oral mucosa-attached film was observed to have a concentration-dependent inhibitory ability to inhibit Tyrosinase activity, and in view of these results, the oral mucosa-attached film according to the present invention had a significant effect on whitening. It can be concluded that there is.

[Experimental Example 5] Skin wrinkle improvement effect of oral mucosa-attached film

In order to evaluate the effect of improving skin wrinkles in humans, an expert's visual evaluation (8 point scale) was conducted. Twenty healthy women with an average age of 47 years (46.80±3.32) were allowed to use two films a day on the inner cheeks of the oral cavity for 12 weeks, and then visually evaluated the wrinkles of the eyes. Scores from 0 to 7 were given according to the degree of wrinkles, and specific evaluation criteria are shown in Table 1 below.

Rating Description 0 None One None / Mild 2 Mild 3 Mild / Moderate 4 Moderate 5 Moderate /Severe 6 Severe 7 Very Severe

8 is a graph evaluating the effect of improving skin wrinkles of the oral mucosa-attached film according to the present invention. Referring to FIG. 8, the experimental results will be described with reference to FIG. 8, after 20 healthy women with an average age of 47 years (46.80±3.32) were allowed to use two films a day on the inner cheeks of the oral cavity for 12 weeks, and the skin wrinkles were examined. Improvement of wrinkles around the eyes could be observed with the naked eye, and in light of these results, it can be seen that the oral mucosa-attached film according to the present invention has a significant effect on improving skin wrinkles.

[Experimental Example 6] Effect of improving skin elasticity of oral mucosa-attached film

In order to evaluate the effect of improving skin elasticity in humans, skin elasticity was measured using a skin elasticity meter. Twenty healthy women with an average age of 47 years (46.80±3.32) attach two films a day to the inner cheeks of the oral cavity and use them for 12 weeks. Changes in the skin surface were measured.

FIG. 9 is a graph showing the results of evaluating the effect of improving skin elasticity of the oral mucosa-attached film according to the present invention. Referring to FIG. 9, the experimental results will be described. After attaching two films a day to the cheeks inside the oral cavity to use for 12 weeks, the skin elasticity was measured using a Reviscometer. As a result, statistically significant skin elasticity effect was observed at 12 weeks. It can be seen that the oral mucosa-attached film according to the invention is effective in improving skin elasticity.

Claims (7)

Low-molecular weight collagen peptide 1.5-22.0% ^{by weight, with a molecular weight of 1×10 4} dalton or less, which exhibits skin aging prevention, wrinkle improvement, skin elasticity, and whitening functions,
0.1-1.0% by weight of elastin peptide that exhibits the function of increasing moisture retention, epidermal flexibility and improving skin elasticity,
50.0-54.0% by weight of water-soluble flulan having adhesiveness and film-forming properties,
10.0-13.0% by weight of modified starch of any one of acid-treated modified starch, hydroxypropylated modified starch, and α-modified starch with modified original structure or physical properties,
Glycerin 10.0-13.0% by weight, which absorbs moisture in the air and imparts the function as a moisturizing agent,
0.6-2.0% by weight of carrageenan contained in order to increase the tensile strength of the film,
6.2-13.5% by weight of aspartame, which masks the bitter taste even when applied in the oral cavity,
Natural extract complex consisting of 15.0-32.0% by weight of vitamin tree fruit extract with anti-inflammatory and antioxidant activity, 65.0-75.0% by weight of blackberry leaf extract, 3.0-10.0% by weight of perilla leaf extract, 1.0-1.5% by weight,
0.1-0.5% by weight of sodium polyacrylate exhibiting hydrophilic and hygroscopic functions; Oral mucosa-attached film composition comprising a low-molecular collagen peptide and a natural extract complex, characterized in that consisting of.
delete delete delete delete delete An oral mucosa-attached film comprising a low-molecular collagen peptide ^{having a molecular weight of 1×10 4} dalton or less and a natural extract complex prepared by using the composition according to claim 1.

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