KR102534346B1 - Cosmetic composition comprising a concentrate of collagen ingredients isolated from apricot - Google Patents

Abstract

It relates to a cosmetic composition and a manufacturing method thereof, and since the composition contains a high content of amino acids constituting collagen, it can be usefully used for preventing, improving or treating skin-related conditions.

Description

Cosmetic composition comprising a concentrate of collagen ingredients isolated from apricot}

It relates to a cosmetic composition and a manufacturing method thereof.

Apricot ( Prunus armeniaca ) is a plant belonging to the genus Rosaceae and is called a passerby in oriental medicine, and has many uses and medicinal effects, such that more than 200 treatment methods using apricot seeds have been recorded in Herbal Medicine and Donguibogam. In particular, herbal external preparations made by grinding apricot seeds are used for skin pigmentation such as spots and freckles, swelling, swelling, etc., and make the skin white and shiny.

Recently, interest in vegan, clean beauty, etc. has increased, and at the same time, consumer preference for clean and safe natural materials such as organic and non-GMO is increasing. In addition, interest in health functional foods and inner beauty products such as edible fish collagen and edible hyaluronic acid is increasing. In particular, consumers' interest in anti-aging in the field of skin care is increasing.

Therefore, there is a need to develop clean and safe vegetable raw materials that can replace chemical and animal materials while having anti-aging effects.

One aspect is to provide a cosmetic composition comprising an amino acid concentrate isolated from apricots as an active ingredient.

Another aspect is to provide a composition for external application for skin comprising an amino acid concentrate isolated from apricot as an active ingredient.

Another aspect is to provide a health functional food composition comprising an amino acid concentrate isolated from apricots as an active ingredient.

Another aspect is mixing vegetable enzymes with dried apricots; and hydrolyzing amino acids in the apricot mixed with the enzyme.

One aspect provides a composition comprising an amino acid concentrate isolated from apricots as an active ingredient. In one embodiment, the amino acid may be a component constituting collagen.

As used herein, the term "collagen" is a type of protein and constitutes bone, skin, cartilage, connective tissue, etc. depending on the type. Numerous collagen synthesis genes are involved in collagen synthesis, and matrix metalloproteinases (MMPs) that degrade connective tissue components including collagen exist. Aging reduces collagen synthesis and increases the activity of MMPs enzymes that decompose collagen, which increases the degradation of collagen, reducing elasticity and increasing wrinkles. Amino acids constituting collagen are mostly hydroxyproline, proline, and glycine. Accordingly, the composition according to one aspect may include, as an active ingredient, hydroxyproline, proline, and glycine, or a mixture thereof constituting collagen as amino acids isolated from apricots. The "hydroxyproline" is an amino acid component in which a hydroxyl group (-OH) is attached to the carbon at position 4 of proline and is contained in a large amount in collagen. The hydroxyproline is involved in promoting the contraction of collagen fibers and protecting elastin, and helps to manage elastic and lively skin by protecting the skin from active oxygen that promotes skin aging. In addition, the "Proline" is one of the aliphatic amino acids constituting protein and accounts for most of collagen together with hydroxyproline. The proline plays a role in conditioning the skin to help manage shiny and elastic skin. In addition, the "glycine (Glycine)" is an essential amino acid constituting collagen, has excellent moisturizing power, and quickly penetrates into skin cells to give luster and moisture to the skin.

In one embodiment, the amino acid concentrate isolated from apricots increases the content of anti-aging and anti-wrinkle factors, such as COL1A1, or reduces the expression of MMP-1, which is increased by ultraviolet rays, and skin moisturizing and skin barrier-related factors. It was confirmed that the expression of hyaluronan synthase 3 (HAS3) or aquaporin 3 (AQP3) was increased. Therefore, a composition comprising an amino acid concentrate isolated from apricot may have a skin beauty improvement effect, for example, skin aging inhibition, skin barrier strengthening, skin wrinkle inhibition, or skin moisturizing effect.

In one embodiment, the composition may be for improving the skin condition or improving skin beauty. For example, the skin condition or skin beauty improvement may be suppression or improvement of skin aging, anti-wrinkle, skin elasticity, skin regeneration, skin barrier strengthening, or skin moisturizing.

As used herein, the term "skin aging" refers to both tangible and intangible changes that appear in the skin with age, such as thinning of the epidermis, number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle , wound healing, skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical substance elimination ability, immune response, sensory function, and decrease in body temperature control. In particular, wrinkles and loss of elasticity are the main symptoms of skin aging, which are known to be caused by the reduction of collagen and elastin, which are the main proteins in the dermis, and the network deformation of these elastic fibers. Elastin and fibronectin, which exist in the dermis and epidermis, form a dense network to maintain the skin cushion layer. loses its holding power.

The composition may be used to improve skin aging caused by exogenous factors or endogenous factors. The extrinsic factor refers to various external factors, such as ultraviolet rays (light), and the endogenous factor is also referred to as a chronological factor and refers to a factor mainly caused by the passage of time. That is, the skin aging is specifically a natural aging phenomenon that occurs as the proliferation of skin cells decreases with age as well as premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemicals, etc. It is a concept that includes wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include stimulation caused by changes in internal and external factors that cause wrinkles by changing components constituting skin tissue.

The aging may be photoaging. As used herein, the term "photoaging" is a phenomenon caused by external environmental factors, and the most representative factor is ultraviolet rays. Ultraviolet rays cause damage to biocomponents such as activation of proteolytic enzymes, chain scission of matrix proteins, and abnormal cross-linking, and repetition of these mechanisms results in apparent skin aging.

As used herein, the term "wrinkle" refers to a state in which elasticity of the skin is lost and loosened, and for example, the skin may be folded. The "prevention or improvement of skin wrinkles" may mean any action of preventing or improving wrinkles by suppressing the expression of wrinkle-related factors or increasing the total amount of collagen.

The "strengthen the skin barrier" may refer to any action that enhances the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients. In addition, "damaged skin barrier function" may refer to all changes in the skin due to a decrease or damage to the function of the skin barrier. Examples include increased skin wrinkling, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne, and the like.

The "moisturizing skin" may refer to any action that maintains skin moisture or prevents moisture loss.

In one embodiment, the composition may be a cosmetic composition.

The cosmetic composition may have, for example, softening lotion, nutrient lotion, massage cream, nutrient cream, essence, pack, gel, ampoule, or skin-adhesive cosmetic formulation.

Ingredients included in the cosmetic composition may include ingredients commonly used in cosmetic compositions other than the composition as active ingredients, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and flavors. can include

In another embodiment, the composition may be a composition for external application for skin.

The external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed. The external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin. Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix|blended suitably.

In another embodiment, the composition may be a health functional food composition.

The health functional food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of health functional food. Among the types of health functional foods, beverage compositions may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The health food composition may also contain nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonated beverages. carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverages, fruit flesh for preparing vegetable beverages, or a combination thereof.

Another aspect provides a method for preventing, improving or treating a skin condition in a subject comprising treating or administering to a subject in need thereof an effective amount of the composition. Details of the skin condition are as described above.

As used herein, the terms "administering," "introducing," and "implanting" are used interchangeably and according to one embodiment into a subject by a method or route that results in at least partial localization of a composition to a desired site. It may mean the arrangement of the composition according to one embodiment of.

Administration may be administered by a method known in the art. Administration can be administered directly to a subject by any means, for example, by routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. can The administration may be administered systemically or locally.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be a subject in need of skin beauty improvement, for example, skin moisturizing, skin barrier strengthening, and skin wrinkle improvement effects.

The administration is 0.1 mg to 1,000 mg per day of the composition according to one embodiment, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg , 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered. However, the dosage may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and reaction sensitivity, and those skilled in the art can Dosage can be appropriately adjusted in consideration of these factors. The number of administrations can be once a day or twice or more within the range of clinically acceptable side effects, and the administration site can be administered to one or more than two sites, daily or every 2 to 5 days, total The number of administration days may be administered from 1 day to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period. For non-human animals, the same dosage per kg as for humans is used, or the above dosage is converted by the volume ratio (eg, average value) of the organ (heart, etc.) between the target animal and the human. A single dose can be administered.

Another aspect is mixing vegetable enzymes with dried apricots; and hydrolyzing amino acids in the apricot mixed with the enzyme.

In one embodiment, the enzyme may be a natural vegetable enzyme. The enzyme may be derived from papain, pineapple, pear, fig, ginger, asparagus, kiwi, mint, melon, etc., and may be, for example, papain, chymopapain, bromelain, etc. there is.

In addition, in the step of hydrolyzing the amino acid, an air bubble brewing process may be performed for 4 to 48 hours.

In the present specification, the term "air bubble brewing" is a technique for generating fine air bubbles of 1 μm to 5 mm in size in water, and oxygen gas in water at a pressure higher than atmospheric pressure supplied from the outside. bubbles can be formed. By performing the air bubble brewing process, there is an effect of promoting hydrolysis of amino acids by physical stirring.

In one embodiment, the air bubble brewing process is 4 to 48 hours, 4 to 36 hours, 4 to 24 hours, 12 to 24 hours, 18 to 36 hours, 18 to 30 hours, 24 to 36 hours, or 24 to 24 hours. It can be performed for 32 hours. At this time, when the air brewing process takes less than the above range, there is a problem in that hydrolysis does not proceed smoothly, so that hydroxyproline, proline, glycine and / or mixtures thereof, which are constituent amino acids of collagen, are not sufficiently produced. When the above range is exceeded, there is a problem in that amino acids decomposed from apricots not only decay or deteriorate, but also increase processing costs.

In one embodiment, compared to apricot extract, dried and mixed apricots with vegetable enzymes were subjected to an air bubble brewing process to isolate amino acids, resulting in increased COL1A expression, decreased expression of MMP-1, elastin, and HAS3. And it was confirmed that the effect of increasing AQP3 expression was remarkable. Therefore, the amino acid isolated according to the method of one aspect has a significantly higher content of hydroxyproline, proline and glycine constituting collagen compared to the apricot extract, and anti-aging activity, skin barrier strengthening, and skin moisturizing by the amino acid activity is excellent.

Since the composition according to one aspect contains a high content of amino acids constituting collagen, it can be usefully used for preventing, improving, or treating skin-related conditions.

1 is a graph showing the effect of a concentrate according to one embodiment on the expression of MMP-1.
Figure 2 is a graph showing the effect of the concentrate according to one embodiment on the expression of COL1A1.
3 is a photograph of the position and amount of production of collagen and elastin in cells observed with a fluorescent antibody.
Figure 4 is a photograph of the location and production of elastin in cells observed through a fluorescent antibody.
Figure 5 is a graph showing the effect of the concentrate according to one embodiment on the expression of HAS3.
Figure 6 is a graph showing the effect of the concentrate according to one embodiment on the expression of AQP3.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

[Example]

Example 1. Preparation of Apricot Amino Acid Concentrate

The apricots were naturally dried and then washed. Thereafter, the dried apricots were mixed with natural plant enzymes and then subjected to an air bubble brewing process for generating fine air bubbles in a water bath for 24 hours to hydrolyze the collagen component in the flesh of the apricots. After separating and extracting the hydrolyzed collagen component and filtering twice to obtain a filtrate, only the concentrated collagen component was isolated. Thereafter, the concentrated collagen components were lyophilized for 12 to 24 hours to prepare an apricot concentrated collagen component powder. The prepared powder was sterilized by irradiating gamma rays of 15 kGy.

[Comparative example]

Comparative Example 1. Preparation of apricot extract

The apricots were naturally dried and then washed. Thereafter, an apricot extract was prepared using dried apricots with 50° C. water.

[Experimental example]

Experimental Example 1. Confirmation of content of collagen components

The content of collagen components contained in the apricot amino acid concentrate of Example 1 was confirmed through High Performance Liquid Chromatography (HPLC) analysis. Specifically, after injecting 1.0 μl of the concentrate prepared in Example 1 into the column, the solvent was flowed at a flow rate of 2.0 ml/min. At this time, the solvent was composed of two mobile phases, 40 mM Phosphate buffer pH 7.8 (solvent A) and methanol / acetonitrile / water (45/45/10, v / v, solvent B), and the following concentration gradient conditions was used: 50-100% solvent A (20 min). In addition, the detection wavelengths were 262 nm and 338 nm. The extract of Comparative Example 1 was also tested in the same way.

ingredient Example 1 Comparative Example 1 Hydroxyproline 48.34 mg/kg 19.59 mg/kg Proline 178.78 mg/kg 66.58 mg/kg Glycine 41.04 mg/kg 0.2 mg/kg

As a result, as shown in Table 1, Example 1 had significantly higher contents of hydroxyproline, proline, and glycine than Comparative Example 1. Specifically, in Example 1, the contents of hydroxyproline, proline, and glycine were measured to be 2.5 times, 2.7 times, and 200 times higher than those of Comparative Example 1, respectively.

That is, it can be seen that the apricot concentrate according to one embodiment has a significantly higher content of amino acids constituting collagen than the apricot extract.

Experimental Example 2. Anti-aging activity 1

The effect of the apricot amino acid concentrate of Example 1 on factors related to aging skin aging and wrinkle formation was analyzed.

Specifically, a human dermal fibroblast cell line (Hs68) was cultured in DMEM (Dulbecco's modified Eagle's medium) containing 10% fetal bovine serum (FBS) and 1% penicillin, and the culture was carried out at 37°C, 5% CO ₂ and It was performed for 24 hours in an incubator at 95% humidity. Thereafter, the medium was removed, DPBS was added, and 20 mJ/cm 2 of UVB was irradiated or not irradiated. Immediately after UVB irradiation, DPBS was removed and replaced with FBS-free medium, and Example 1 and Comparative Example 1 were treated at different concentrations (10 ppm, 100 ppm) and further cultured for 24 hours. Pure distilled water was added as a negative control group and 1 µM EGCG was added as a positive control group. Thereafter, cells in each medium were obtained, washed with cold PBS, and RNA was isolated using an RNAprep kit (Monarch®Total RNAprep Kit, NEB, USA), RNA was quantified at 260 nm by nanodrop, and each 2 μg cDNA was synthesized in an amplifier using the RNA of (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, cybergreen (SYBR Green supermix, Applied Biosystems, USA) was added together with primers (see Table 2 below) and cDNA for the target genes COL1A1 and MMP-1, followed by real-time PCR. Real-time polymerase chain reaction was performed on a machine (Step One Plus, Applied Biosystems, USA). The real-time PCR reaction was performed by repeating 40 cycles of polymerase activation at 94°C for 5 minutes, followed by 95°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds. The expression levels of the COL1A1 and MMP-1 genes were finally analyzed through correction for the β-actin gene, and the results are shown in FIGS. 1 and 2, respectively.

gene forward primer reverse primer COL1A1 5′-GAGGGCCAAGACGAAGACATC-3′ 5'-CAGATCACGTCATCGCACAAC-3' MMP-1 5′-CGAATTGCCGACAGAGATGA-3′ 5′-GTCCCTGAACAGCCCAGTACTT-3′ β-actin 5′-GGCCATCTCTTGCTCGAAGT-3′ 5′-GAGACCTTCAACACCCCAGC-3′

1 is a graph showing the effect of an amino acid concentrate according to one embodiment on the expression of MMP-1, and FIG. 2 is a graph showing the effect of an amino acid concentrate according to one embodiment on the expression of COL1A1.

As a result, as shown in FIG. 1, it was confirmed that Example 1 and Comparative Example 1 reduced the expression of MMP-1, which was increased by ultraviolet light, in a concentration-dependent manner. In particular, in the case of Example 1, compared to Comparative Example 1, it was confirmed that the MMP-1 expression level was significantly reduced. In addition, as shown in Figure 2, it was confirmed that Example 1 and Comparative Example 1 increased the expression of COL1A1 in a concentration dependent manner. In particular, in the case of Example 1, it was confirmed that the COL1A1 expression level significantly increased compared to Comparative Example 1.

That is, the amino acid concentrate according to one aspect inhibits the production of MMP-1 increased by ultraviolet rays and increases collagen, thereby having an excellent effect on preventing skin aging such as reducing elasticity and suppressing wrinkle formation.

Experimental Example 3. Anti-aging activity 2

The intracellular location and amount of production of Type 1 collagen and elastin in the cells cultured in the above experimental example were observed under a confocal microscope.

Specifically, the cell line of Experimental Example 1 was dispensed at 3.0×10 ⁴ cells/well into a 4-well lab-tek chambered cover glass coated with Poly-L-Lysine, and cultured for 24 hours at 37 °C and 5% CO ₂ conditions. Thereafter, the medium was removed, washed with PBS, replaced with Serum free media, and cultured for 48 hours at 37°C, 5% CO ₂ conditions. Thereafter, the medium was removed and fixed with 10% neutral buffered formalin for 10 minutes, followed by permeabilization with 0.5% Trixon X-100. Then, after adding 1% BSA (0.01 g/ml in PBS), blocking was performed at room temperature for 1 hour, and the primary antibodies, Anti-collagen 1 antibody and Anti-Elastin antibody, were added and incubated overnight at 4°C. did The next day, secondary antibodies, Anti-Rabbit and Anti-Mouse Antibody, were added and stained while blocking light for 1 hour at room temperature. Then, after removing the Lab-tek cover, 1 drop of VECTASHIELD® MOUNTING MEDIUM with DAPI was added, covered with a coverslip, fixed by applying nail polish around the area, and dried completely to prepare a sample for observation. Thereafter, fluorescence of the sample was observed using a confocal microscope (Olympus FV 1200). Ascorbic acid was used as a positive control.

FIG. 3 is a photograph of the location and amount of intracellular collagen type I and elastin observed with a fluorescent antibody.

Figure 4 is a photograph of the location and production of elastin in cells observed through a fluorescent antibody.

As a result, as shown in FIG. 3, it was confirmed that Example 1 showed strong fluorescence intensities of elastin (red) and collagen type I (green) compared to Comparative Example 1. In addition, as shown in FIG. 4, Example 1 showed a stronger fluorescence intensity of elastin (red) compared to Comparative Example 1, and it was confirmed that the fluorescence intensity was stronger compared to the positive control group.

That is, the amino acid concentrate according to one aspect can help increase skin elasticity by promoting the production of collagen and elastin, which are factors affecting skin elasticity.

Experimental Example 4. Skin barrier strengthening and skin moisturizing activity

The effect of the amino acid concentrate of Example 1 on skin barrier strengthening and skin moisturizing activity was analyzed.

Specifically, human keratinocyte HaCaT cells were cultured in DMEM medium (Dulbecco's modified Eagle's Medium, Gibco 1210-0038) containing 10% fetal bovine serum and 1% penicillin, and the culture was All were performed in an incubator at 37°C, 5% CO ₂ and 95% humidity. The cultured cell lines were treated with Example 1 and Comparative Example 1 at different concentrations (10 ppm, 100 ppm) and further cultured for 24 hours. As a positive control, 1 μM of retinoic acid was used. Thereafter, the cells in each medium were collected, washed with chilled PBS, RNA was isolated using an RNAprep kit (Monarch® Total RNAprep Kit, NEB, USA), RNA was quantified at 260 nm with nanodrop, and 2 μg each cDNA was synthesized in an amplifier using the RNA of (C1000 Thermal Cycler, Bio-Rad, USA). The synthesized cDNA was added as a template along with primers (see Table 3 below) and cDNA for HAS3 (hyaluronic acid synthase) and AQP3 (aquaporin synthesis protein), which are factors related to strengthening and moisturizing the skin barrier, which are target genes. Real-time polymerase chain reaction was performed in a real-time PCR machine (Step One Plus, Applied Biosystems, USA). The real-time PCR reaction was performed by repeating 40 cycles of polymerase activation at 94°C for 5 minutes, followed by 95°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds. The expression levels of the HAS3 and AQP3 genes were finally analyzed through correction for the β-actin gene, and the results are shown in FIGS. 1 and 2 .

gene forward primer reverse primer HAS3 5′-CTTAAGGGTTGCTTGCTTGC-3′ 5′-GTTCGTGGGAGATGAAGGAA-3′ AQP3 5′-GTCACTCTGGGCATCCTCAT-3′ 5′-CTATTCCAGCACCCAAGAAGG-3′ β-actin 5′-GGCCATCTCTTGCTCGAAGT-3′ 5′-GAGACCTTCAACACCCCAGC-3′

Figure 5 is a graph showing the effect of the amino acid concentrate according to one embodiment on the expression of HAS3, Figure 6 is a graph showing the effect of the amino acid concentrate according to one embodiment on the expression of AQP3.

As a result, as shown in FIGS. 5 and 6, it was confirmed that Example 1 and Comparative Example 1 significantly increased the expression of HAS3 and AQP3 in a concentration-dependent manner. In particular, in Example 1, it was confirmed that the effect of increasing HAS3 and AQP3 expression was remarkable compared to Comparative Example 1 at the same concentration.

Therefore, the composition according to one aspect can restore the damaged skin barrier and enhance skin defense by strengthening the skin barrier. In addition, it is excellent in moisturizing the skin and is effective in preventing or treating diseases related to moisturizing.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

Claims (8)

A cosmetic composition comprising an amino acid concentrate isolated from apricot as an active ingredient, wherein the amino acid is obtained by performing air bubble brewing. The cosmetic composition according to claim 1, wherein the amino acid constitutes collagen. The cosmetic composition according to claim 1, wherein the amino acid is selected from the group consisting of Hydroxyproline, Proline, and Glycine. The method according to claim 1, skin condition improvement, or cosmetic composition for moisturizing. The cosmetic composition according to claim 4, wherein the skin condition is skin barrier enhancement, skin wrinkles, skin elasticity, or skin regeneration. A composition for external application for skin for improving skin wrinkles or skin elasticity, comprising an amino acid concentrate isolated from apricot as an active ingredient, wherein the amino acid is obtained by performing air bubble brewing. A health functional food composition comprising an amino acid concentrate isolated from apricot as an active ingredient, wherein the amino acid is obtained by performing air bubble brewing. mixing vegetable enzymes with dried apricots; and
A method for separating amino acids constituting collagen from apricots, comprising: performing air bubble brewing to hydrolyze amino acids from apricots mixed with the enzyme.

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