KR101305274B1 - Composition for improving collagen synthesis - Google Patents

Abstract

The present invention discloses a composition for promoting collagen synthesis, including collagen hydrolyzate, protein hydrolyzate, vitamins and minerals. The composition not only has excellent absorption in the body, but also has excellent safety properties. The present invention also discloses a composition for preventing skin aging, preventing and improving skin wrinkles, wound healing or skin tissue repair and regeneration comprising the composition as an active ingredient.

Description

Composition for improving collagen synthesis {Composition for improving collagen synthesis}

The present invention relates to a composition for promoting collagen synthesis.

Ingredients currently used as functional materials in the field of skin care include hyaluronic acid for beauty, hypericin for atopic prevention, N-acetyl-, D-glucosamine for wound healing or moisturizing, grape seed for whitening or wound healing Examples include extracts, isoflavones, ginkgo extracts, ceramides, astaxanthin, horse riding, coenzyme Q10, chondroitin, lycopene, hesperidin, alpha lipoic acid, mandarin powder, cranberry, and the like. However, since the credible standards and specifications for the functional ingredients are not set, the difference between the quality and efficacy of products including the same is large.

On the other hand, the development trends related to functional ingredients are focused on the development of a single substance with proven functionality, and even if the functionality of a single substance is clarified, its dose is limited due to its toxicity. Most people do not have side effects.

The present invention is to provide a composition that is non-toxic and has a high collagen synthesis effect excellent in body absorption, and further provides a composition for preventing skin aging, preventing and improving skin wrinkles, wound healing or skin tissue repair and regeneration including the composition. .

One aspect of the present invention provides a composition for promoting collagen synthesis, including collagen hydrolyzate, protein hydrolyzate, vitamins and minerals.

Another aspect of the present invention provides a composition for preventing skin aging, preventing and improving skin wrinkles, wound healing or skin tissue repair and regeneration comprising the composition as an active ingredient.

The composition according to one aspect of the present invention includes a collagen hydrolyzate, a protein hydrolyzate, vitamins and minerals, thereby providing a composition for promoting collagen synthesis having no toxicity and excellent absorption in the body. In addition, by including the composition as an active ingredient, it is possible to provide a composition for preventing skin aging, preventing and improving skin wrinkles, healing wounds, repairing and regenerating skin tissue.

1 is a standard curve graph of a kit used for evaluating collagen synthesis promoting effect of dermal fibroblasts.
Figure 2 is a graph comparing the collagen synthesis amount of skin fibroblasts according to the sample (* <0.05, ** <0.001).

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a concept of the broadest notion including a scalp and hair as well as a tissue covering a body surface such as a face or a body.

Hereinafter, the present invention will be described in detail.

Currently, compositions containing collagen are applied to products for preventing skin aging and skin aging, but there are doubts about their actual effects because no appropriate standard is set for the evaluation of functionalities and exertion of efficacy in vivo. Accordingly, the present inventors have developed a low molecular weight composition composed of components that are harmless to the human body and have no side effects, considering the collagen synthesis mechanism of the dermal fibroblasts as a molecular biological unit, and confirmed the efficacy through functional examination using cell experiments, and optimized The weight ratio was derived to complete the present invention.

One aspect of the present invention provides a composition for promoting collagen synthesis, including collagen hydrolyzate, protein hydrolyzate, vitamins and minerals. In particular, the composition according to one aspect of the present invention may promote collagen synthesis in the body. In addition, collagen hydrolyzate, protein hydrolyzate, vitamins and minerals in the composition according to an aspect of the present invention can ensure excellent safety because they are harmless and non-toxic components to humans and animals.

In the present specification, "collagen" is a protein constituting the largest amount in the human body, quantitatively and functionally, the extracellular matrix forming the skeleton of all the cells in the human body (extracellular matrix), more specifically the connective tissue (connective tissue) Means a configuration factor. Human tissues containing large amounts of collagen include bone tissue such as skin, bone and cartilage, tendons, ligaments, and the like. Aging of the tissues, such as skin wrinkles and sagging, osteoporosis due to human aging diseases, etc., which is known to be due to a decrease in the amount of collagen and function in the body due to reduced collagen synthesis of tissues.

The main functions of dermal fibroblasts include the regeneration of damaged skin tissue through extracellular matrix synthesis and proliferation, including endogenous aging factors such as photoaging, accumulation of damage caused by free oxygen, and cellular aging due to telomeres. Aging factors reduce the physiological activity of dermal fibroblasts in the dermis. Type I collagen, which accounts for more than 95% of the extracellular matrix of the skin and plays a very important part in the physiological activity of fibroblasts through interactions and signal exchanges with cell adhesion molecules and cytoskeleton, etc. When the synthesis of Hg decreases, the amount of collagen in the skin tissue is reduced, thereby reducing the surface tension to form skin wrinkles, and causing a vicious cycle in which the bioactive functions including the proliferation and regeneration of cells are reduced. do. Therefore, collagen of dermal fibroblasts may be directly related to tissue repair or regeneration during skin aging, skin wrinkle formation and skin damage.

"Procollagen" is a precursor of collagen found in the skin and other organs of many vertebrates, and as a result, functions the same as collagen in the body.

That is, if the synthesis of collagen or procollagen of dermal fibroblasts is promoted, it is possible to prevent and improve skin wrinkles, heal wounds, repair and regenerate damaged skin tissues, and prevent and improve skin aging.

In the present specification, "collagen hydrolyzate" includes collagen itself or collagen-derived compositions processed by acid or base treatment, heat treatment, enzyme treatment, or the like to collagen or gelatin. As used herein, the term "collagen peptide (peptide)" is a collagen or gelatin decomposed by an enzyme, etc., means a low molecular weight collagen hydrolyzate having 2 to 20 amino acid residues. The enzyme may also be peptine or collagenase.

It is known that oral ingestion of gelatin or collagen hydrolysates extracted from other animals (mammals or fish) results in the transfer of collagen di- and tri- peptides into the blood (Iwai, K. et al. , J. Agric. Food Chem . , 2005, 53 (16), 6531-6536). In addition, collagen peptides have been reported to act as matrikine, a signaling material for collagen synthesis of dermal fibroblasts and osteoblasts in humans (Maquart, F. et. al ., Crit Rev Onco / Hematol. 2004, 49: 199-202). Since the amount of matrikain decreases in the blood as aging progresses, it is known that it is directly related to the inhibition of collagen synthesis and the decrease of turnover. Thus, when collagen hydrolyzate is applied, it can increase the amount of matrixicaine in the blood and further promote collagen synthesis.

On the other hand, the collagen composition administered by the oral route is absorbed by the Peptide transporter (PEPT1) in the state of a low molecular peptide with 2-5 amino acid residues and transferred to the blood. When applied by the transdermal route, it is known that the molecular weight that can be absorbed to the dermal layer containing the fibroblasts that synthesize collagen is about 500 Da (HAE Benson et al., Letters in Peptide Science , 10: 615-620, 2003). Thus, it would be desirable for compositions comprising collagen administered orally and transdermally to have low molecular weight in order to interact substantially with fibroblasts in the body. However, since most of the collagen compositions commercially available have a high molecular weight, which is much higher than the average molecular weight of 1000 Da, there is a limit to the absorption and physiological activity.

The composition according to one aspect of the present invention may include collagen hydrolyzate to promote collagen synthesis. The composition according to another aspect of the present invention may include gelatin to promote collagen synthesis.

In one aspect of the invention, the collagen hydrolyzate comprises a collagen peptide. In another aspect of the invention the collagen peptide comprises a collagen dipeptide, collagen tripeptide or collagen oligopeptide (oligo-peptide).

In one aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 1000 Da or less. In another aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 1 to 1000 Da. In another aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 500 to 1000 Da. In another aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 1 to 800 Da. In another aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 500 to 800 Da. In another aspect of the invention, the average molecular weight of the collagen hydrolyzate may be 1 to 500 Da.

In one aspect of the invention, the collagen hydrolyzate includes that obtained in mammals or fish. In another aspect of the present invention, the collagen hydrolyzate includes those obtained from the skin of a mammal or from the young (scales) or skin of a fish. In another aspect of the invention, the mammal comprises a cow or a pig, and the fish comprises a blowfish or a bream.

Collagen hydrolyzate according to an aspect of the present invention may be prepared by the method for preparing collagen hydrolyzate disclosed in Korean Patent Application No. 10-2008-0065511, but is not limited thereto.

The mechanism of collagen synthesis in dermal fibroblasts is largely characterized by ① collagen gene transcription initiation, ② transcription (mRNA synthesis), ③ translation (protein synthesis), and ④ translation. It is based on a five-step process of protein gene expression: post-translational modifications, ⑤ secretion and further modification. There is a limit to inducing collagen synthesis, so if administered together to promote collagen synthesis, collagen synthesis may be further promoted.

On the other hand, amino acids have a collagen synthesis promoting effect. Among them, glutamine, in particular, has an effect of increasing collagen synthesis and increases collagen synthesis at wound sites of humans or animals. Vitamins, hormones, cytokines, and minerals can also stimulate collagen synthesis.

The composition according to one aspect of the present invention may include protein hydrolysate to promote collagen synthesis. As used herein, a “protein hydrolyzate” includes a protein-derived composition processed by acid or base treatment, heat treatment, enzymatic treatment, or the like on the protein itself. In another aspect of the invention, the protein hydrolyzate comprises wheat gluten hydrolyzate or amino acid. In another aspect of the invention, the amino acid comprises arginine, glutamine, L-glutamine, arginine peptide or glutamine peptide.

As described above, it will be desirable for the oral and transdermal compositions to have low molecular weight in order to interact substantially with fibroblasts in the body. In one aspect of the invention, the average molecular weight of the protein hydrolyzate may be 1000 Da or less. In another aspect of the present invention, the average molecular weight of the protein hydrolyzate may be 1 to 1000 Da. In another aspect of the invention, the average molecular weight of the protein hydrolyzate may be 500 to 1000 Da. In another aspect of the invention, the average molecular weight of the protein hydrolyzate may be 1 to 800 Da. In another aspect of the invention, the average molecular weight of the protein hydrolyzate may be 500 to 800 Da. In another aspect of the invention, the average molecular weight of the protein hydrolyzate may be 1 to 500 Da.

The composition according to one aspect of the present invention may include vitamins to promote collagen synthesis. In another aspect of the invention, the vitamin is a concept that includes all the ingredients that can be classified as a vitamin, specifically may be vitamin C.

The composition according to one aspect of the present invention may include minerals to promote collagen synthesis. In the present specification, “mineral” may also be referred to as an inorganic salt, and refers to an inorganic component of an organism except carbon, hydrogen, and oxygen among the elements constituting the organism. In another aspect of the invention, the mineral comprises calcium, phosphorus, potassium, sodium, chlorine, magnesium, iron, iodine, copper, zinc, cobalt, manganese ions. In another aspect of the invention, the mineral may be calcium ions (Ca ^{2 +} ).

In one aspect of the present invention, the composition comprises a low molecular weight collagen hydrolyzate and protein hydrolyzate having an average molecular weight of 1000 Da or less, vitamins and minerals with a lower molecular weight, so that the oral intake is immediately absorbed in the intestine, and the blood transfer rate is high, When the epithelium is applied, the skin transmittance is excellent and the absorption is high. Therefore, even small amounts can effectively promote collagen synthesis.

In one aspect of the invention, the composition may comprise collagen hydrolyzate, protein hydrolyzate, vitamins and minerals in a specific ratio. In another aspect of the invention, the composition comprises 50 to 95% by weight collagen hydrolyzate, 4 to 50% by weight protein hydrolysate, 0.1 to 30% by weight vitamin and 0.1 to 30% by weight based on the total weight of the composition Contains minerals. In another aspect of the invention, the composition comprises 60 to 80% by weight collagen hydrolyzate, 15 to 45% by weight protein hydrolyzate, 0.1 to 10% by weight vitamin and 0.1 to 10% by weight based on the total weight of the composition May comprise% minerals. In another aspect of the invention, the composition comprises 65 to 75% by weight collagen hydrolyzate, 20 to 35% by weight protein hydrolyzate, 0.1 to 5% by weight vitamin and 0.1 to 5% by weight based on the total weight of the composition May comprise% minerals. When used in the above range is not only suitable for showing the intended effect of the present invention, it can satisfy both the stability and safety of the composition, it may be appropriate to use in the above range in terms of cost-effectiveness. More specifically, one aspect of the present invention discloses that by identifying a specific ratio of the components, the effective components can be used efficiently within a safe and stable range.

The composition according to one aspect of the present invention includes the composition promoting the synthesis of collagen as an active ingredient to prevent and improve skin wrinkles, heal wounds, repair and regenerate damaged skin tissue, prevent and improve skin aging can do. That is, one aspect of the present invention provides a composition for preventing skin aging, preventing and improving skin wrinkles, wound healing or skin tissue repair and regeneration comprising the collagen synthesis promoting composition as an active ingredient. In particular, the composition according to one aspect of the present invention may be used for the purpose of reducing or ameliorating symptoms before or after the appearance of signs of skin aging or wrinkle formation. The composition according to another aspect of the present invention can be used for the purpose of more effective skin regeneration when the wound or burn damage to the skin.

One aspect of the present invention provides a food composition comprising the composition for promoting collagen synthesis. In one aspect of the present invention, the composition may contain other components and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, additives such as perfumes, pigments, fungicides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers and synthetic polymer materials may be further included to improve physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. For example, the addition amount of the components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight based on the total weight of the composition. In one aspect of the invention, the food composition includes, but is not limited to, health food compositions, functional food compositions, supplements, processed food compositions, food additives and the like.

One aspect of the present invention provides a cosmetic composition comprising the composition for promoting collagen synthesis. The cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. emulsions obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, emulsions obtained by dispersing the aqueous phase in oil phases, multi emulsions, suspensions, micro emulsions, micro Capsules, microgranulocytes, ionic (liposomal) and nonionic vesicle dispersants, foams, aerosols or patches containing further compressed propellant may be used. These compositions may be prepared according to conventional methods in the art.

The cosmetic composition may contain other ingredients in addition to the above substances in a range that does not impair the main effect, and preferably may have a synergistic effect on the main effect, and other cosmetics in addition to the active ingredient of the present invention Depending on the formulation of the composition or the purpose of use, those skilled in the art can appropriately select and blend without difficulty. For example, the cosmetic composition of the present invention may include other ingredients which are usually formulated into the cosmetic composition as necessary in addition to the active ingredient, and examples thereof include oil-fat ingredients, humectants, emollients, surfactants, organic and inorganic pigments, organic Powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, stabilizers, thickeners, glycerin, pH regulators, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, limiting agents, purified water and the like. Other compounding components that may be included in the cosmetic composition is not limited thereto, and the compounding amount of the above components is possible within a range that does not impair the object and effect of the present invention.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected as desired. For example, supple lotion, nourishing lotion, essence, nourishing cream, massage cream, pack, gel, makeup base, foundation, powder, lipstick, patch, spray, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, One selected from the group consisting of cleanser, hair shampoo, hair conditioning, hair treatment, hair essence, hair lotion, scalp hair tonic, scalp essence, hair gel, hair spray, hair pack, body lotion, body cream, body oil and body essence It may be prepared in the above formulation, but is not limited thereto.

One aspect of the present invention provides a pharmaceutical composition comprising the composition for promoting collagen synthesis. Such pharmaceutical compositions may contain, in addition to the active ingredient, preservatives, stabilizers, hydrates, emulsifiers, salts and / or buffers for osmotic pressure control, diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine), Glidants such as silica, talc, stearic acid and magnesium or calcium salts or polyethylene glycols thereof or binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpi Pharmaceutical adjuvant or other therapeutically useful substances, such as rolidine). Optionally, other pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, or sweeteners such as starch, agar, alginic acid or sodium salts thereof may be included.

The pharmaceutical compositions can be formulated in various oral or parenteral dosage forms according to conventional methods. The parenteral administration agent may be, for example, formulations of drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, injections, and the like, but is not limited thereto.

The pharmaceutical composition according to one aspect of the present invention may be administered orally, parenterally, for example, transdermal, intravenous and the like. The composition is based on the formulation and criteria suitable for each administration. In particular, in the case of intravenous injection, all additives not suitable for this are excluded, and the purity of the composition itself is also very high.

The applied amount of the active ingredient will vary depending on the age, sex, weight, disease, pathological condition, route of administration and prescriber's judgment of the subject to be treated. Application amount determination based on these factors is within the level of skill in the art. Typical dosages may be 5-15 g, but are not limited thereto.

One aspect of the present invention also provides a composition including, but not limited to, a laboratory reagent or a medium composition, a composition for household goods, a composition for clothing, including the composition for promoting collagen synthesis.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples. However, these Examples, Comparative Examples and Experimental Examples are provided only for the purpose of illustration in order to facilitate understanding of the present invention, and the scope and scope of the present invention is not limited by the following Examples, Comparative Examples and Experimental Examples.

[Examples 1 to 9 and Comparative Examples]

(1) Preparation of Collagen Peptides

Collagen peptide compositions with high molecular weight oligopeptide content were prepared from the skin (bream) or skin of sea bream or puffer fish or cow or pig skin. Raw materials introduced into the reactor were treated with base (1N NaOH, Na ₂ SO ₄ and NaHCO ₃ ) and acid (1N HCl and H ₂ SO ₄ ), and pretreated by sterilization through atmospheric heating. Complex proteolytic enzymes composed of an endo type protease and an exo type peptidase were treated at 2% by weight per total weight. After hydrolysis, collagen peptides were prepared by sequentially undergoing purification processes such as decolorization and deodorization, micro-, ultra-, and nano-filter systems. Example 1 It was set as. The peptide produced by the above method is composed of low molecular oligopeptides having an average molecular weight of about 800 Da or less.

(2) Vitamin C (Ascorbic acid) or Ca ^{2 +} containing composition

Vitamin C (ascorbic acid) and CaCl ₂ were commercially available and obtained in Examples 2 and 3, respectively.

(3) Preparation of Glutamine Peptides

Using low gluten high glutamine content as a raw material instead of the skin of fish or mammals, a low molecular glutamine peptide having an average molecular weight of 500-1000 Da was prepared in the same manner as in the collagen peptide of (1) to be Example 4.

(4) Preparation of Mixed Composition

Examples 1 to 4 prepared above were mixed as in the composition of Table 1 to prepare Examples 5 to 9.

(5) Comparative Example

It was a commercially available collagen powder manufactured amino collagen ^® in Japan Meiji Seika as a comparative example.

Examples 1 to 9 and the control, the specific composition of the comparative example is shown in Table 1 below.

division Constituent Component concentration Negative control group No treatment Basal (DMEM) medium Positive control group TGF-beta 10 nmol / ml TGF-β Comparative example Amino Collagen ^® 150 μg / ml product powder  Example 1 Collagen Peptides 150 μg / ml collagen peptide Example 2 Vitamin C 1 μg / ml L-ascorbic acid Example 3 CaCl ₂ (Ca ^{2 +} ) 1 μg / ml CaCl ₂ Example 4 Glutamine Peptides 50 μg / ml Glutamine Peptide Example 5 Collagen Peptides + Vitamin C 149 μg / ml collagen peptide + 1 μg / ml L-ascorbic acid Example 6 Collagen Peptides + Glutamine Peptides 100 μg / ml collagen peptide + 50 μg / ml glutamine peptide Example 7 Collagen Peptides + Vitamin C + Ca ^{2 +} 148 μg / ml collagen peptide + 1 μg / ml L-ascorbic acid + 1 μg / ml CaCl ₂ Example 8 Collagen Peptides + Vitamin C + Glutamine Peptides 100 μg / ml collagen peptide + 1 μg / ml L-ascorbic acid + 49 μg / ml glutamine peptide Example 9 Collagen Peptides + Vitamin C + Glutamine Peptides + Ca ^{2 +} 100 μg / ml collagen peptide + 1 μg / ml L-ascorbic acid + 48 μg / ml glutamine peptide + 1 μg / ml CaCl ₂

Experimental Example Evaluation of Collagen Synthesis Promoting Effect of Skin Fibroblasts

Human Dermal Fibroblast (HDF) was added Dulbeccos's Minimum Essential with 10% (volume / volume) Fetal Bovine Serum (FBS) and 1% (volume / volume) penicillin and streptomycin. Incubate in Medium (DMEM) to maintain Passage 5 or less, and change the amount of collagen synthesis according to the above Example or Comparative Example treatment in vitro ( in in vitro ) to evaluate the collagen synthesis promoting effect of human dermal fibroblasts.

Seed human dermal fibroblasts in a well plate at a density of 1 x 10 ⁴ cells / cm ² and incubator (37 ° C, 5% CO) for 24 hours for cell attachment and environmental adaptation. ₂ ) were incubated. After the medium was removed, the medium without serum was treated and starvation was carried out for 8 hours. The control, Example and Comparative Example compositions were dissolved in a medium and treated for each sample. After the sample treatment, the human skin fibroblasts were cultured for 72 hours, and the supernatant of the culture medium was taken to quantify the synthesized collagen. Collagen quantification was performed using a Type 1 Procollagen C-Peptide EIA kit (Takara, Japan). The standard curve of the kit used for quantification of collagen is shown in FIG. 1, and the experimental results are shown in Table 2 and FIG. 2.

As can be seen in Table 2 and Figure 2, Examples 1 to 9 all significantly increased the amount of collagen synthesis compared to the negative control. Especially, the collagen synthesis increase effect of Examples 5-9 which combined Examples 1-4 was more excellent. In particular, Example 9 including all of Examples 1 to 4 shows that the collagen synthesis increase rate of about 235% when the collagen synthesis amount of the negative control to 100% shows the most excellent collagen synthesis increase effect. In addition, it shows excellent collagen synthesis increasing effect compared to the comparative example, Example 9 has a significantly superior collagen synthesis promoting effect in that the comparative example further includes whey protein, gelatin, etc. which can have a positive effect on the promotion of collagen synthesis It can be confirmed that has.

Hereinafter, a formulation example of a food composition, a cosmetic composition, and a pharmaceutical composition comprising the composition for promoting collagen synthesis according to one aspect of the present invention as an active ingredient will be described in more detail, but the food composition, the cosmetic composition, and the pharmaceutical composition are provided in various formulations. It is applicable and not intended to limit the invention, but rather to explain in detail.

Formulation Example 1 Preparation of Beverage

Example ..... 1000 mg

Citric acid ..................... 1000 mg

Oligosaccharide ......... 100 g

Plum concentrate ......... 2 g

Taurine ............... 1 g

Purified water is added to the whole ............ 1000 ml

The above ingredients are mixed according to a conventional beverage preparation method, followed by stirring and heating at 85 ° C. for about 1 hour. The resulting solution was obtained by filtration in a sterilized 2 L container, sealed sterilized and then refrigerated to be used for preparing the beverage composition of the present invention.

Formulation Example 2 Cream

The cream may be prepared according to a conventional method as shown in Table 3 below.

Compounding ingredient weight% Example 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hardened castor oil 2.0 Liquid paraffin 10 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Suitable amount Pigment Suitable amount Spices Suitable amount Purified water to 100

Formulation Example 3 Preparation of Tablet

Examples ............... 50 mg

Corn starch ........... 100 mg

Lactose ...................... 100 mg

Magnesium Stearate ......... 2 mg

After mixing the above components is prepared by tableting according to a conventional tablet production method.

Formulation Example 4 Preparation of Injection

Example ............... 200 mg

Sterile Distilled Water for Injection ...

pH adjuster ...

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Claims (10)

Collagen synthesis comprising 65 to 75 wt% collagen peptide, 20 to 35 wt% gluten peptide derived from wheat gluten, 0.1 to 5 wt% vitamin C and 0.1 to 5 wt% calcium, based on the total weight of the composition Promotional cosmetic composition. The method of claim 1,
Collagen peptide cosmetic composition for promoting collagen synthesis, characterized in that the average molecular weight of 1000 Da or less. The method of claim 1,
Collagen peptide is a cosmetic composition for promoting collagen synthesis, characterized in that it comprises a mammal or fish obtained. The method of claim 1,
Cosmetic composition for promoting collagen synthesis, characterized in that the average molecular weight of the glutamine peptide is 1000 Da or less. A cosmetic composition for preventing skin aging, preventing and improving skin wrinkles, promoting wound healing or promoting skin tissue regeneration, comprising the composition according to any one of claims 1 to 4 as an active ingredient.
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