KR102168053B1 - 고속 온 디맨드 미세유체 액적 생성 및 조작 - Google Patents

고속 온 디맨드 미세유체 액적 생성 및 조작 Download PDF

Info

Publication number
KR102168053B1
KR102168053B1 KR1020157029843A KR20157029843A KR102168053B1 KR 102168053 B1 KR102168053 B1 KR 102168053B1 KR 1020157029843 A KR1020157029843 A KR 1020157029843A KR 20157029843 A KR20157029843 A KR 20157029843A KR 102168053 B1 KR102168053 B1 KR 102168053B1
Authority
KR
South Korea
Prior art keywords
delete delete
microfluidic
droplets
microfluidic droplet
fusion component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020157029843A
Other languages
English (en)
Korean (ko)
Other versions
KR20150131345A (ko
Inventor
유-천 쿵
페이-유 이 치우
팅-샹 에스 우
위에 천
마이클 에이 테이텔
Original Assignee
더 리전트 오브 더 유니버시티 오브 캘리포니아
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 더 리전트 오브 더 유니버시티 오브 캘리포니아 filed Critical 더 리전트 오브 더 유니버시티 오브 캘리포니아
Publication of KR20150131345A publication Critical patent/KR20150131345A/ko
Application granted granted Critical
Publication of KR102168053B1 publication Critical patent/KR102168053B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00599—Solution-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00713—Electrochemical synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/00756—Compositions, e.g. coatings, crystals, formulations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)
  • Molecular Biology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020157029843A 2013-03-15 2014-03-13 고속 온 디맨드 미세유체 액적 생성 및 조작 Active KR102168053B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361798516P 2013-03-15 2013-03-15
US61/798,516 2013-03-15
PCT/US2014/026185 WO2014151658A1 (en) 2013-03-15 2014-03-13 High-speed on demand microfluidic droplet generation and manipulation

Publications (2)

Publication Number Publication Date
KR20150131345A KR20150131345A (ko) 2015-11-24
KR102168053B1 true KR102168053B1 (ko) 2020-10-20

Family

ID=51581005

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157029843A Active KR102168053B1 (ko) 2013-03-15 2014-03-13 고속 온 디맨드 미세유체 액적 생성 및 조작

Country Status (8)

Country Link
US (2) US10071359B2 (enExample)
EP (1) EP2972405B1 (enExample)
JP (1) JP6461905B2 (enExample)
KR (1) KR102168053B1 (enExample)
CN (1) CN105452873B (enExample)
AU (1) AU2014233699B2 (enExample)
CA (1) CA2909687A1 (enExample)
WO (1) WO2014151658A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT202100014972A1 (it) 2021-06-09 2021-09-09 Iodo S R L Sistema microfluidico a liquidi espansi per la produzione di drug carrier

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US9176504B2 (en) 2011-02-11 2015-11-03 The Regents Of The University Of California High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
GB2539836B (en) 2012-08-13 2017-03-29 Univ California Methods for detecting target nucleic acids in sample lysate droplets
US10071359B2 (en) 2013-03-15 2018-09-11 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
CN105441309B (zh) * 2014-08-27 2018-04-27 中国科学院苏州纳米技术与纳米仿生研究所 循环式单细胞捕获转移芯片
CN105441308B (zh) * 2014-08-27 2018-03-20 中国科学院苏州纳米技术与纳米仿生研究所 循环式单细胞捕获芯片
WO2016064755A2 (en) * 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
WO2016133783A1 (en) * 2015-02-17 2016-08-25 Zalous, Inc. Microdroplet digital pcr system
CN106290492A (zh) * 2015-05-11 2017-01-04 宁波大学 驱动用构件易拆解并可循环再用的霍乱诊断用装置
US9855555B2 (en) 2015-05-20 2018-01-02 University Of Maryland Generation and trapping of aqueous droplets in a microfluidic chip with an air continuous phase
CN106290856A (zh) * 2015-05-26 2017-01-04 宁波大学 四通道并且双驱动耦合运作的艾滋病诊断用微流控装置
CN106290931A (zh) * 2015-05-26 2017-01-04 宁波大学 以廉价疏水材质作基片的亚型猪流感检测用多通道装置
CN106290857A (zh) * 2015-05-26 2017-01-04 宁波大学 多通道且双驱动耦合运作的霍乱诊断用微流控芯片装置
CN106290932A (zh) * 2015-05-26 2017-01-04 宁波大学 包含疏水基片的双驱动耦合的艾滋病诊断用多通道装置
CN106290933A (zh) * 2015-05-26 2017-01-04 宁波大学 双机制驱动耦合运作的霍乱诊断用微流控芯片装置
CN106290930A (zh) * 2015-05-26 2017-01-04 宁波大学 含疏水基片的双驱动耦合的亚型猪流感多通道检测装置
CN105136763B (zh) * 2015-09-10 2017-10-13 大连海事大学 基于气液界面单细胞捕获及叶绿素荧光表征的单微藻细胞活性动态监测新方法与装置
CN106556708A (zh) * 2015-09-24 2017-04-05 宁波大学 两种驱动方式耦合运作的含疏水基片的微流控芯片装置
EP3269444A1 (en) * 2016-07-14 2018-01-17 Base4 Innovation Ltd Method of identifying droplets in a stack and an associated sequencer
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US11376595B2 (en) 2016-11-30 2022-07-05 Pilot Gene Technologies (Hangzhou) Co., Ltd. Droplet digital PCR chip
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
EP3645167A4 (en) 2017-06-28 2021-02-24 Bio-rad Laboratories, Inc. DROPLET DETECTION SYSTEM AND METHOD
CN107694475B (zh) * 2017-09-25 2021-02-19 南京航空航天大学 一种微纳物质的环状聚集物成形装置
US20200376740A1 (en) 2017-10-03 2020-12-03 Cornell University Systems and methods for micropatterning objects
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
CN107904155B (zh) * 2017-10-19 2021-06-22 广州市第一人民医院 一种用于液体分散的微流控芯片及其应用、使用方法
US11045805B2 (en) 2017-11-01 2021-06-29 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
JP7030361B2 (ja) * 2017-11-06 2022-03-07 北京新▲い▼生物科技有限公司 微小液滴生成装置
WO2020002197A1 (en) 2018-06-27 2020-01-02 Institut Curie Method of inducing or preventing the merging of droplets
EP3586964A1 (en) 2018-06-27 2020-01-01 Inorevia Method of manipulating droplets in a channel
US11173464B2 (en) 2018-07-30 2021-11-16 Hrl Laboratories, Llc Methods for dissolving droplet particle assembly with co-solvent, and particle assemblies obtained therefrom
US11167287B2 (en) 2018-07-30 2021-11-09 Hrl Laboratories, Llc Dissolving droplet microfluidic particle assembly devices and methods, and particle assemblies obtained therefrom
US11845061B1 (en) 2018-07-30 2023-12-19 Hrl Laboratories, Llc Complex structures obtained from dissolving-droplet nanoparticle assembly
EP3613498A1 (en) * 2018-08-24 2020-02-26 Université de Liège Microfluidic module for co-encapsulation in droplets
CN109456890A (zh) * 2018-11-23 2019-03-12 大连理工大学 一种分层带状共培养4种肝细胞的微流控芯片及其应用
CN111215159B (zh) * 2018-11-26 2024-10-25 上海欣戈赛生物科技有限公司 微流控芯片及基于该芯片融合样本的方法
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
US20220250077A1 (en) * 2019-07-24 2022-08-11 Hewlett-Packard Development Company, L.P. Digital microfluidics device with droplet processing components
US12194660B2 (en) 2019-08-30 2025-01-14 Drexel University 3D capillary self-assembly of long aspect ratio particles
US12372465B2 (en) 2019-11-15 2025-07-29 Scintimetrics, Inc. Compositions and methods based on diffusion of fluorophores
CN115485068A (zh) * 2020-01-24 2022-12-16 康比纳蒂股份有限公司 用于微流体装置制造的方法和系统
US11376812B2 (en) 2020-02-11 2022-07-05 Helicoid Industries Inc. Shock and impact resistant structures
US12275227B2 (en) 2020-02-11 2025-04-15 Helicoid Industries, Inc. Composite materials and structures
CN115279482B (zh) * 2020-03-13 2024-12-13 国立研究开发法人科学技术振兴机构 微液滴、气泡生成装置
CN112871227B (zh) * 2021-01-07 2022-10-11 中国科学院青岛生物能源与过程研究所 基于光热效应进行微量液滴操控的微流控芯片及方法
US11852297B2 (en) 2021-06-01 2023-12-26 Helicoid Industries Inc. Containers and methods for protecting pressure vessels
US11346499B1 (en) 2021-06-01 2022-05-31 Helicoid Industries Inc. Containers and methods for protecting pressure vessels
CN113652333B (zh) * 2021-08-03 2023-03-21 中国科学院上海微系统与信息技术研究所 一种优化流体分布的微柱式多相位移通道
JP2025526269A (ja) 2022-06-27 2025-08-13 ヘリコイド インダストリーズ インコーポレイテッド 空気力学的構造物の前縁保護のための高耐衝撃性強化繊維
US11802870B1 (en) 2022-12-13 2023-10-31 Panazee Kinetic immunoassay systems and methods
US12030053B1 (en) 2023-05-05 2024-07-09 Panazee Inc. Multi-step kinetic immunoassay systems and methods
CN119633919A (zh) * 2024-12-31 2025-03-18 利德健康科技(广州)有限公司 液滴生成结构、微流控流道、微流控芯片及液滴生成方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523142A (ja) 2003-04-10 2006-10-12 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体種の形成および制御
JP2010054492A (ja) 2008-05-13 2010-03-11 Sony Corp マイクロチップとその流路構造
JP2010169551A (ja) 2009-01-23 2010-08-05 Foundation For The Promotion Of Industrial Science 駆動部を有する微小液滴の配列装置

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2832117B2 (ja) 1991-11-29 1998-12-02 キヤノン株式会社 サンプル測定デバイス及びサンプル測定システム
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US20060128006A1 (en) 1999-11-10 2006-06-15 Gerhardt Antimony L Hydrodynamic capture and release mechanisms for particle manipulation
CA2390375A1 (en) 1999-11-10 2001-05-17 Massachusetts Institute Of Technology Cell analysis and sorting apparatus for manipulation of cells
JP2001158099A (ja) 1999-12-02 2001-06-12 Fuji Xerox Co Ltd インクジェット記録ヘッド及びインクジェット記録装置
US6481453B1 (en) * 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
AU2001290879A1 (en) 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
GB0105831D0 (en) 2001-03-09 2001-04-25 Toumaz Technology Ltd Method for dna sequencing utilising enzyme linked field effect transistors
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP3610349B2 (ja) 2002-08-06 2005-01-12 キヤノン株式会社 液体搬送装置
US7582482B2 (en) 2002-09-03 2009-09-01 Dionex Corporation Continuous ion species removal device and method
CN1261767C (zh) 2003-01-30 2006-06-28 财团法人工业技术研究院 低电压低功率热气泡薄膜式微流体驱动装置
GB0315438D0 (en) * 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
EP1663460B1 (en) 2003-09-04 2015-07-08 Premium Genetics (UK) Limited Multiple laminar flow-based particle and cellular separation with laser steering
FR2873171B1 (fr) 2004-07-19 2007-12-07 Centre Nat Rech Scient Cnrse Circuit microfluidique a composant actif
US20060073035A1 (en) * 2004-09-30 2006-04-06 Narayan Sundararajan Deformable polymer membranes
WO2006076567A2 (en) 2005-01-13 2006-07-20 Micronics, Inc. Microfluidic rare cell detection device
JP4047336B2 (ja) 2005-02-08 2008-02-13 独立行政法人科学技術振興機構 ゲル電極付セルソーターチップ
US7918244B2 (en) 2005-05-02 2011-04-05 Massachusetts Institute Of Technology Microfluidic bubble logic devices
JP5114702B2 (ja) 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
WO2007024914A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
CN101715483A (zh) * 2007-02-05 2010-05-26 微芯片生物工艺学股份有限公司 微流体和纳米流体装置、系统和应用
JP4539707B2 (ja) 2007-10-25 2010-09-08 ソニー株式会社 微小粒子分取装置及び微小粒子分取用基板、並びに微小粒子分取方法
US8062903B2 (en) 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
KR100955845B1 (ko) * 2008-05-15 2010-05-04 재단법인서울대학교산학협력재단 액적 병합장치 및 이를 이용한 액적 병합 방법
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
KR100967414B1 (ko) * 2008-11-27 2010-07-01 한국과학기술원 유체 방울 혼합용 미세 유체 제어 장치 및 이를 이용하여 유체 방울을 혼합하는 방법
WO2010092845A1 (ja) 2009-02-13 2010-08-19 コニカミノルタホールディングス株式会社 マイクロ流路構造体及びマイクロポンプ
KR101125060B1 (ko) * 2009-07-22 2012-03-21 한국과학기술원 입자를 포획하는 미세유체 소자 및 이를 이용한 입자 포획 방법
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US8563325B1 (en) 2009-09-29 2013-10-22 Sandia Corporation Coaxial microreactor for particle synthesis
US20110114190A1 (en) 2009-11-16 2011-05-19 The Hong Kong University Of Science And Technology Microfluidic droplet generation and/or manipulation with electrorheological fluid
US9772261B2 (en) 2010-02-12 2017-09-26 Fluidion Sas Passive micro-vessel and sensor
BR112012020285B1 (pt) 2010-02-12 2020-12-29 Dan Angelescu dispositivo, ferramenta, sistema e método para comunicar sua posição por emissão acústica
US20130217113A1 (en) 2010-07-15 2013-08-22 Advanced Liquid Logic Inc. System for and methods of promoting cell lysis in droplet actuators
US9176504B2 (en) 2011-02-11 2015-11-03 The Regents Of The University Of California High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
GB201211342D0 (en) * 2012-06-26 2012-08-08 Cambridge Entpr Ltd Microfluidic device
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US10071359B2 (en) 2013-03-15 2018-09-11 The Regents Of The University Of California High-speed on demand microfluidic droplet generation and manipulation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523142A (ja) 2003-04-10 2006-10-12 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体種の形成および制御
JP2010054492A (ja) 2008-05-13 2010-03-11 Sony Corp マイクロチップとその流路構造
JP2010169551A (ja) 2009-01-23 2010-08-05 Foundation For The Promotion Of Industrial Science 駆動部を有する微小液滴の配列装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT202100014972A1 (it) 2021-06-09 2021-09-09 Iodo S R L Sistema microfluidico a liquidi espansi per la produzione di drug carrier

Also Published As

Publication number Publication date
US10071359B2 (en) 2018-09-11
KR20150131345A (ko) 2015-11-24
AU2014233699A1 (en) 2015-11-05
CN105452873B (zh) 2019-01-18
EP2972405A4 (en) 2016-10-19
AU2014233699B2 (en) 2018-05-24
US20190060861A1 (en) 2019-02-28
WO2014151658A1 (en) 2014-09-25
CA2909687A1 (en) 2014-09-25
US10780413B2 (en) 2020-09-22
EP2972405B1 (en) 2020-12-23
US20160051958A1 (en) 2016-02-25
JP6461905B2 (ja) 2019-01-30
CN105452873A (zh) 2016-03-30
JP2016515214A (ja) 2016-05-26
EP2972405A1 (en) 2016-01-20

Similar Documents

Publication Publication Date Title
KR102168053B1 (ko) 고속 온 디맨드 미세유체 액적 생성 및 조작
EP2812709B1 (en) High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
US10226768B2 (en) Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
CN107107058A (zh) 高清晰度微液滴打印机
US20180297028A1 (en) Self-powered microfluidic chip with micro-patterned reagents
EP3000528B1 (en) Microfluidic device for the generation of combinatorial samples
Hoefemann et al. Sorting and lysis of single cells by BubbleJet technology
US20230053160A1 (en) Selective and High-Resolution Printing of Single Cells
Quan et al. Robust and rapid partitioning in thermoplastic
Yoshida et al. Highly efficient combination of multiple single cells using a deterministic single-cell combinatorial reactor
Akhtar A microsystem for on-chip droplet storage and processing
Kung High-Speed Dielectrophoresis and 3D Microfluidics for Biological Applications
Schneider Studies of Sample Compartmentalization By Microfluidic Methods

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20151015

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20181102

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20200103

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20200715

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20201014

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20201014

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20240927

Start annual number: 5

End annual number: 5