KR102093238B1

KR102093238B1 - 살모넬라 타이피뮤리움의 증식을 억제하는 신규 박테리오파지 및 이의 용도

Info

Publication number: KR102093238B1
Application number: KR1020180110234A
Authority: KR
Inventors: 박미경; 최인영; 장동욱; 박도현
Original assignee: 경북대학교 산학협력단
Priority date: 2018-09-14
Filing date: 2018-09-14
Publication date: 2020-05-22
Also published as: KR20200031339A

Abstract

본 발명은 살모넬라 타이피뮤리움에 특이적인 사멸능을 나타내는 신규 박테리오파지 및 이를 포함하는 항균 조성물에 관한 것이다. 또한, 본 발명은 상기 박테리오파지 또는 상기 항균 조성물을 이용하여 감염성 질병 예방용 조성물, 항생제, 소독제 등 다양하게 활용하는 방법에 관한 것이다.

Description

살모넬라 타이피뮤리움의 증식을 억제하는 신규 박테리오파지 및 이의 용도{Novel bacteriophage having pathogen Salmonella Typhimurium specific antibacterial activity and use thereof}

본 발명은 새롭게 분리 및 동정된 신규 파지, 이를 유효성분으로 포함하는 살모넬라 타이피뮤리움(Salmonella Typhimurium)의 증식 억제 또는 사멸용 조성물, 및 살모넬라 타이피뮤리움균 감염성 질병 예방용 조성물, 항생제, 소독제에 관한 것이다.

살모넬라(Salmonella)는 일정한 유사 성향을 가지고 있는 군의 세균류에 붙여진 속명으로서 이 명칭은 1885년에 D. E. Salmon이 그의 공동연구자 Theobald Smith와 같이 돼지 Cholera bacillus를 발견한 것을 기념하여 이와 유사한 세균에 대해 일괄적으로 명명한 것이다. 그 후 1888년 Gartner가 독일에서 발생한 식중독에서 같은 균을 발견하게 되었는데, 이것이 Salmonella 식중독에 대한 최초의 보고가 되었다. 현재 Salmonella 속은 2,500여 종 이상의 혈청형이 보고 되었으며, 동물에 숙주 특이성을 나타내는 균종과 숙주특이성이 없는 균종으로 분류할 수 있다. 이 중, 인체에 해가 되는 식중독을 유발하는 혈청형은 숙주특이성이 없는 Salmonella Typhimurium과 Salmonella Enteritidis 등이 대표적이며, Salmonella Typhimurium이 그 중에서도 가장 높은 비율(20%)을 차지하고 있다.

따라서, 현재 살모넬라의 예방 및 사멸을 위해 항생제를 주로 사용하고 있으나, 최근 많은 분야에서 항생제 내성균의 출현이 문제가 되고 있다. 이로 인해 산업동물의 배합 사료 내에서는 항생제의 사용을 금지를 시작하였으며, 우리나라에서는 2011년 하반기부터 규제하였다. 따라서 항생제를 대체할 수 있는 친환경 소재의 탐색 및 이를 기반으로 한 효과적인 제어법에 대한 연구가 다양한 방면에서 요구되고 있다. 이러한 연구 중 하나는 자연계에 널리(10³⁰-10³²) 존재하는 박테리오파지 분리하여 이의 억제능을 검토하는 것이다.

박테리오파지(bacteriophage)는 특정 세균을 숙주로 하여 생존하는 바이러스의 일종으로 단일 혹은 이중 사슬의 DNA(Deoxyribonucleic acid) 또는 RNA(Ribonucleic acid)를 유전 물질로 포함한다. 세균 내부로 침투한 박테리오파지가 증식하여 생성된 자손 박테리오파지들이 세균으로부터 밖으로 방출되기 위해 세포벽을 파괴함으로써 세균을 사멸시킨다. 이는 항생제와 비교하였을 때, 숙주로 하는 세균 외에는 영향을 미치지 않는 높은 선택성, 지속성, 낮은 부작용 및 항생제 대비 개발 비용 및 시간이 효율적이라는 측면에서 우위를 차지할 수 있다.

따라서, 축산업 및 식품 산업에서 막대한 경제적 손실을 유발하는 살모넬라에 의한 식중독 발병을 예방 또는 치료를 위해 살모넬라 타이피뮤리움에 특이적인 용균성을 갖는 박테리오파지 및 이의 응용에 대한 개발이 절대적으로 필요하다.

대한민국 등록특허 (0001) KR 10-1260655

이에, 본 발명자들은 항생제의 오남용으로 인한 항생제 내성균의 문제점 및 식품 내의 항생제의 잔류 문제 및 과거 항생제와 같이 광범위한 숙주범위의 문제점을 해결하기 위해, 살모넬라 타이피뮤리움(Salmonella Typhimurium)을 사멸시킬 수 있는 신규 박테리오파지를 자연계에서 분리하고 분리된 신규 박테리오파지 KFS-ST의 형태학적, 생화학적 및 유전학적 특성을 검토하여 상기 박테리오파지가 다양한 환경에서 살모넬라 타이피뮤리움을 특이적으로 사멸시킬 수 있음을 확인하였다.

본 발명의 목적은 살모넬라 타이피뮤리움(Salmonella Typhimurium)에 특이적으로 사멸능을 가지는 박테리오파지 KFS-ST(KCTC 13465BP)를 제공하는 것이다.

본 발명의 다른 목적은 살모넬라 타이피뮤리움균에 감염하여 살모넬라 타이피뮤리움균을 사멸시킬 수 있는 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 살모넬라 타이피뮤리움균의 감염을 방지하는 데에 활용 가능한 조성물 및 이 조성물을 이용한 살모넬라 타이피뮤리움균의 감염 방지 방법을 제공하는 것이다.

본 발명의 또 다른 목적은 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 항생제, 소독제 또는 세척제를 제공하는 것이다.

상기 목적을 달성하기 위하여, 본 발명은 살모넬라 타이피뮤리움(Salmonella Typhimurium)에 특이적으로 사멸능을 가지는 박테리오파지 KFS-ST(KCTC 13465BP) 제공한다.

또한, 본 발명은 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 항생제를 제공한다.

또한, 본 발명은 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 소독제 또는 세척제를 제공한다.

삭제

본 발명의 박테리오파지 KFS-ST는 살모넬라 타이피뮤리움균의 감염에 따른 질병의 예방에 유용하게 이용할 수 있을 것이다.

도 1은 살모넬라 타이피뮤리움균 ATCC 13076에 대한 KFS-ST의 용균 활성을 나타낸 것이다.
도 2는 신규 박테리오파지 KFS-ST의 투과전자 현미경 사진이다.
도 3은 신규 박테리오파지 KFS-ST의 온도 안정성 실험 결과를 나타낸 것이다.
도 4는 신규 박테리오파지 KFS-ST의 pH 안전성 실험 결과를 나타낸 것이다.
도 5는 신규 박테리오파지 KFS-ST의 유기용매 안정성 실험 결과를 나타낸 것이다.
도 6은 신규 박테리오파지 KFS-ST의 세척제에 따른 안정성 실험 결과를 나타낸 것이다.
도 7은 신규 박테리오파지 KFS-ST의 단백질 패턴을 확인한 결과를 나타낸 것이다.
도 8은 신규 박테리오파지 KFS-ST의 유전자의 크기를 전기영동 결과를 나타낸 것이다.
도 9는 신규 박테리오파지 KFS-ST의 시험분석(challenge assay) 결과를 나타낸 것이다.
도 10는 신규 박테리오파지 KFS-ST의 저장성 실험 결과를 나타낸 것이다.

이하, 본 발명에 대하여 보다 상세히 설명한다.

본 발명은 살모넬라 타이피뮤리움균을 특이적으로 사멸시킬 수 있는 능력을 갖는 박테리오파지 KFS-ST(KCTC 13465BP), 및 이를 유효성분으로 포함하는 조성물을 이용하는 살모넬라 타이피뮤리움균의 감염 방지 및 살모넬라 타이피뮤리움균에 의해 유발되는 질환에 대한 예방 및 치료방법을 제공한다.

본 발명의 박테리오파지 KFS-ST(KCTC 13465BP)는 본 발명자들에 의해 분리된 후 2018년 01월 22일자로 한국생명공학원구원 생물자원센터에 기탁되었다.

본 명세서에서 사용된 "방지" 또는 "예방"라는 용어는 (ⅰ)살모넬라 타이피뮤리움균 감염의 방지; 및 (ⅱ)살모넬라 타이피뮤리움균 감염에 의한 질병으로의 발전을 억제하는 것을 의미한다.

본 명세서에서 사용된 "치료"라는 용어는 (ⅰ)살모넬라 타이피뮤리움균에 의해 유발된 질환의 억제; 및 (ⅱ)살모넬라 타이피뮤리움균에 의해 유발된 질환의 병적 상태를 경감시키는 모든 행위를 의미한다.

본 명세서의 "분리", "분리한" 또는 "분리된"은 자연 상태로부터 여러 실험 기법을 활용하여 박테리오파지를 분리하는 것과 타 박테리오파지와 구별하여 특정 지을 수 있는 특징을 확보하는 일을 지칭하며, 이에 더하여 생물공학기술로 박테리오파지를 산업적으로 활용할 수 있게끔 증식시키는 것도 포함한다.

본 발명의 명세서에 있어서, 상기 ‘KFS-ST’에는 파지 자체뿐만 아니라 파지 배양물도 포함된다.

본 발명에 따른 KFS-ST는 통상적인 파지 배양법에 의해 대량으로 배양할 수 있다. 배양 배지로는 탄소원, 질소원, 비타민 및 미네랄로 구성된 배지를 사용할 수 있다. 예를 들어, TSB(tryptic soy broth), LB(luria-bertani broth) 와 같은 액체 배지, 또는 TSA(tryptic soy agar) 와 같은 고체배지 등을 사용할 수 있다. 배양은 통상의 배양조건으로 수행할 수 있다. 배양액 중의 배양배지를 제거하고 농축된 균체만을 회수하기 위해 원심분리 또는 여과과정을 거칠 수 있으며 이러한 단계는 당해 기술 분야에서 통상의 지식을 가진 자의 필요에 따라 수행할 수 있다. 농축된 균체는 통상적인 방법에 따라 냉동하거나 냉동 건조하여 그 활성을 잃지 않도록 보존할 수 있다.

본 발명의 조성물에 포함되는 약제학적으로 허용되는 담체는 제제 시에 통상적으로 이용되는 것으로서, 락토오스, 덱스트로오스, 수크로오스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산칼슘, 알지네이트, 젤라틴, 규산칼슘, 미세결정성 셀룰로오스, 폴리비닐피롤리돈, 셀룰로오스, 물, 시럽, 메틸 셀룰로오스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은아니다. 본 발명의 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다.

본 발명의 조성물은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화 됨으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수도 있다. 이때 제형은 오일 또는 수성 매질 중의 용액, 현탁액 또는 유화액 형태이거나 엑스제, 분말제, 과립제, 정제 또는 캡슐제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수도 있다.

본 발명의 조성물은 활용 방식에 따라, 이에 국한되지 않지만 소독제, 음수첨가제 및 사료첨가제로 구현될 수 있다.

본 발명의 박테리오파지 KFS-ST은 Salmonella Typhimurium ATCC 13311을 감염시키는 박테리오파지로서, 정이십면체의 머리(icosahedral head)를 가지며 꼬리 (tail)가 관찰되는 구조(도 2)를 갖는, 형태학상 시포비리대(Siphoviridae)에 속하는 박테리오파지이다. 박테리오파지 KFS-ST의 단백질 사이즈는 39kDa 및 41kDa으로 확인되었다(도 7). 박테리오파지 KFS-ST의 pH 안전성에 있어서는 pH 6.0부터 pH 11.0까지 활성을 잃지 않고 안정적이었으며(도 4), 온도 안전성에 있어서는 4 ~ 40℃에서 1시간 동안 노출 시 활성을 잃지 않고 유지하였다(도 3). 유기용매인 클로로포름(chloroform) 및 에탄올(ethanol)에서 용균활성을 유지하였으며(도 5), 세척제에 대한 안정성 실험에서는 모든 세척제(0.1% citric acid, 1% citric acid, 0.1% peracetic acid, 1% peracetic acid, 100ppm sodium hypochlorite, 200ppm sodium hypochlorite)에서 용균활성의 감소를 보였으나 citric acid에서는 90% 생존율을 보여 비교적 안정함을 확인하였다(도 6). Challenge assay 결과, 모든 MOI(0.01, 0.1, 1, 10, 100)에서 박테리오파지 KFS-ST 접종 후 배양 2시간 동안 살모넬라 타이피뮤리움의 사멸 및 성장 억제 효과를 보였다(도 9). 박테리오파지 KFS-ST 저장성 실험 결과, KFS-ST는 6주간 0.4 log PFU/ml 이하의 감소를 보였으며, 이 중 -20, 4, 22에서 저장한 KFS-ST가 비교적 안정함을 확인하였다(도 10).

이하, 본 발명에 대하여 보다 상세히 설명한다. 본 명세서에 기재되지 않은 내용은 당해 기술 분야 또는 유사 분야에서 숙련된 자이면 충분히 인식하고 유추할 수 있는 것이므로 그 설명을 생략한다.

< 실시예 1> 살모넬라 타이피뮤리움균에 감염하는 박테리오파지의 분리

<1-1> 대상 박테리아 및 파지의 샘플링

살모넬라 타이피뮤리움(Salmonella Typhimurium)에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해 용균반 형성 여부를 분석하였다. 경상북도 상주 소재 닭 가공 공장의 세척수 25 ml을 시료액으로 준비하고, 이를 이용하여 소프트 아가 오버레이 (soft agar overlay) 방법을 수행하였다. 상기 소프트 아가 오버레이 방법이란 탑-아가 (top-agar, 0.4% 한천을 이용하여 고체 배지 위에 붙이는 것)에서 성장하는 숙주세포를 이용하여 박테리오파지가 용균하는 것을 관찰하는 방법을 말한다.

구체적으로, TSB 배지(tryptone 17.0 g, soytone 3.0 g, glucose 2.5 g, sodium chloride 5.0 g, dipotassium hydrogen phosphate 2.5 g/l) 225ml에 샘플 25ml 혼합물에 살모넬라 타이피뮤리움 (Salmonella Typhimurium) 진탕 배양액 (OD₆₄₀ = 0.2) 1%를 접종하여 37℃에서 160 rpm으로 16시간 동안 배양한 다음, 상기 배양액을 8,000 rpm에서 10분간 원심 분리하여 그 상등액을 0.20 μm 필터로 여과하였다. 여과액의 박테리오파지 존재 여부를 확인하기 위하여, TSA 평판 배지의 위에 TA soft agar [0.4%(w/v)] agar, nutrient broth, NaCl, MgSO₄, CaCl₂) 4 ml와 살모넬라 타이피뮤리움 진탕 배양액 (OD₆₄₀ = 0.2) 200 μl의 혼합액을 부어 굳힌 후, 그 위에 상기 여과액 10 μl를 적가하고 37℃에서 16시간 동안 배양하여 용균반이 형성됨을 확인하였다.

하나의 용균반에는 한 종류의 박테리오파지가 존재한다고 알려져 있기 때문에, 상기 형성된 용균반으로부터 단일 박테리오파지를 분리하고자 하였다. 구체적으로, 상기 용균반을 900 μl의 SM 용액 (50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO₄)에 가하고 실온에서 1시간 동안 방치하여 박테리오파지 용액을 수득하였다.

<1-2> 박테리오파지의 대량 배양 및 정제

상기 실시예 1-1에서 수득한 박테리오파지를 살모넬라 타이피뮤리움균을 이용하여 대량으로 배양하고 이로부터 박테리오파지를 정제하였다.

구체적으로, 살모넬라 타이피뮤리움균을 진탕배양하여 TA broth (nutrient broth, NaCl, MgSO₄, CaCl₂) 3 ml에 분주한 후, 2시간 배양시킨 후, 실시예 1-1에서 수득한 박테리오파지를 분주하여 마찬가지로 2시간 배양시킨 후, 원심분리하여 여과하여 박테리오파지만을 분리하였다. 동일한 방법으로 TA broth의 양을 조절하여 8, 20, 200, 800 ml 순으로 진행한다. 그 다음 최종 농도가 10%(w/v)가 되도록 폴리에틸렌글리콜(polyethylene glycol, PEG)와 1M NaCl을 가하여 4℃에서 16시간 동안 추가 정치시켰으며, 4℃에서 8,000 rpm으로 20분간 원심 분리하여 침전물을 수득하였다.

정제된 파지는 CsCl 밀도 구배 초고속 원심분리 (밀도 구배: 1.20, 1.30, 1.40, 1.45, 1.50, 1.70g/ml) 4℃에서 22,000 rpm으로 2시간 동안 시행하였으며 SM 완충액 (50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO₄)을 사용하여 투석 과정을 거친 후 4℃에서 보관하였다.

본 발명자는 도축장으로부터 시료를 채취하여, 살모넬라 타이피뮤리움균을 특이적 사멸시키는 상기 박테리오파지를 "KFS-ST"로 명명하였다.

< 실시예 2> 분리된 박테리오파지 KFS -ST의 특성 분석

<2-1> 박테리오파지 KFS -ST의 기타 병원성 균주 감염성 비교

상기 실시예 1에서 정제한 박테리오파지 KFS-ST가 살모넬라 타이피뮤리움균 이외에 다른 병원성 균주에 대하여도 용균 활성을 나타내는지의 여부를 확인하기 위해 교차 감염을 수행하였다.

구체적으로, 50종의 병원성 균을 각각 배양하여 각각의 배양액에 대해 상기 정제한 KFS-ST의 용균반 형성 여부를 dot assay, 용균반검사를 수행하여 확인하였다.

그 결과, 하기 표 1에 나타난 바와 같이, Salmonella Typhimurium ATCC 13311, Salmonella Enteritidis ATCC 13076, Shigella boydii NCCP 11190, Shigella flexneri 2457 및 Shigella sonnei ATCC 929이 용균반이 형성되는 것을 확인하였으며, Salmonella Typhimurium ATCC 13311에 대한 용균반 형성이 가장 뛰어난 것을 확인하였습니다(도 1).

균주명	용균반 형성 여부	EOPs**	균주명	용균반 형성 여부	EOPs
Salmonella Typhimurium ATCC 13311	++	1.00	Bacillus toyonensis SR070	-	-
Salmonella Enteritidis ATCC 13076	+	1.00 ± 0.01	Escherichia coli O157:H7 ATCC 43895	-	-
Shigella boydii NCCP 11190	+	0.87 ± 0.02	Escherichia coli O157:H7 700599	-	-
Shigella flexneri 2457	+	0.91 ± 0.01	Escherichia coli O157:H7 rifR	-	-
Shigella sonnei ATCC 9290	+	0.89 ± 0.01	Escherichia coli O157:H7 204p	-	-
Aeromonas hydrophila JUNAH	-	-	Escherichia coli ATCC BAA-2192	-	-
Aeromonas hydrophila SNUFPC A10	-	-	Escherichia coli ATCC BAA-2196	-	-
Aeromonas hydrophila SNUFPC A3	-	-	Escherichia coli BW 25113	-	-
Aeromonas hydrophilaSNUFPC A5	-	-	Escherichia coli K12 ER 2738	-	-
Aeromonas hydrophila SNUFPC A7	-	-	Escherichia coli K12 VSM 1692	-	-
Aeromonas hydrophila SNUFPC A9	-	-	Klebsiella pneumoniae ATCC 13883	-	-
Aeromonas hydrophila SNUFPC A11	-	-	Listeria innocua ATCC 33090	-	-
Bacillus cereus ATCC 13061	-	-	Listeria monocytogenes 1911	-	-
Bacillus cereus ATCC 1611	-	-	Listeria monocytogenes G3982 4b	-
Bacillus cereus ATCC 21768	-	-	Listeria monocytogenes G6055	-	-
Bacillus cereus NCCP 14795	-	-	Listeria monocytogenes H7738	-	-
Bacillus cereus RB002	-	-	Listeria monocytogenes H7757	-	-
Bacillus cereus RB003	-	-	Pseudomonas aeruginosa ATCC 9027	-	-
Bacillus cereus RB005	-	-	Salmonella Heidelberg	-	-
Bacillus cereus RB011	-	-	Salmonella Hartford	-	-
Bacillus cereus RB013	-	-	Salmonella Mission	-	-
Bacillus cereus RB015	-	-	Salmonella Montevideo	-	-
Bacillus cereus RB020	-	-	Salmonella Salamae	-	-
Bacillus cereus RB037	-	-	Salmonella Senftenberg	-	-
Bacillus pumilus SR067	-	-	Shigella sonnei ATCC 9290	-	-
Bacillus safensis SR138	-	-	Staphylococcus aureus ATCC 25923	-	-
Bacillus subtilis KACC 12680	-	-	Vibrio parahaemolyticus ATCC 17802	-	-

<2-2> 온도에 따른 박테리오파지 KFS -ST의 안정성 분석

박테리오파지 KFS-ST의 온도의 안정성을 확인하기 위하여 1.30 × 10⁶ PFU/ml의 KFS-ST를 다양한 온도(-70℃, -20℃, 4℃, 22℃, 37℃, 50℃, 60℃ 및 70℃)에 1시간 동안 노출 시킨 후 용균반검사를 진행하여 박테리오파지의 역가 변화를 측정하였다.

그 결과, 박테리오파지 KFS-ST는 4~40℃에서 온도범위에서 유의적인 차이 없이 안정하게 유지되었다. -20℃, 60℃에서는 유의적인 차이는 보였으나 그 활성이 80% 이상 유지되는 것으로 나타났다. 따라서 박테리오파지 KFS-ST의 경우 넓은 범위의 온도에서 활성이 유지되었다(도 3).

<2-3> pH에 따른 박테리오파지 KFS -ST의 안정성 분석

박테리오파지 KFS-ST의 pH 안정성을 확인하기 위하여 다양한 pH (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 및 12) 용액 900 μl와 3.52 × 10⁷ PFU/ml 농도의 KFS-ST 100 μl를 혼합하여 22℃ 1시간 동안 노출 시킨 후 용균반검사를 진행하여 역가를 측정하였다.

그 결과, 박테리오파지 KFS-ST는 pH 4부터 pH 12까지 유의차 없는 것으로 보아 넓은 범위의 pH에서 활성이 유지되었다(도 4).

<2-4> 유기용매에 따른 박테리오파지 KFS -ST의 안정성 분석

박테리오파지 KFS-ST의 유기용매에 대한 안정성을 확인하기 위해 다양한 유기용매(chloroform, isopropanol 및 ethanol)900 μl와 3.52 × 10⁷ PFU/mL 농도의 KFS-ST 100 μl를 혼합하여 22℃ 1시간 동안 노출 시킨 후 용균반검사를 진행하여 역가를 측정하였다.

그 결과, 박테리오파지 KFS-ST는 시험을 진행한 모든 유기용매(chloroform, isopropanol 및 ethanol)에서 활성이 유지되었다(도 5).

<2-5> 세척제에 따른 박테리오파지 KFS -ST의 안정성 분석

박테리오파지 KFS-ST의 세척제에 대한 안정성을 확인하기 위해 다양한 세척제(citric acid, peracetic acid, sodium hypochlorite)에 노출시켜, KFS-ST의 용균 활성의 변화를 측정하였다.

구체적으로, 각각의 유기용매[0.1% 시트르산(citric acid), 1% 시트르산(citric acid), 0.1% 과산화아세트산(peracetic acid), 1% 과산화아세트산(peracetic acid), 100 ppm 차아염소산나트륨(sodium hypochlorite), 200 ppm 차아염소산나트륨(sodium hypochlorite)] 900 μl와 3.52 × 10⁷ PFU/mL 농도의 KFS-ST 100 μl를 혼합하여 22℃ 1시간 동안 노출 시킨 후 용균반검사를 진행하여 역가를 측정하였다.

그 결과, 모든 세척제(0.1% citric acid, 1% citric acid, 0.1% peracetic acid, 1% peracetic acid, 100ppm sodium hypochlorite, 200ppm sodium hypochlorite)에서 용균활성의 감소를 보였으나 citric acid에서는 박테리오파지 KFS-ST의 생존율이 90%로 비교적 안정함을 확인하였다. 반면, 모든 농도의 peracetic acid에서는 박테리오파지 KFS-ST의 용균활성이 완전히 억제되었다(도 6).

< 실시예 3> 박테리오파지 KFS -ST의 전자현미경 분석

분리된 파지의 형태학적 분류를 위해 분리된 파지를 구리 그리드에 흡수시킨 후에, 4% 포스포텅스텐산 (phosphotungstic acid)로 음성 염색하고 건조하였다. 상기 파지의 형태는 바이오-전자투과 현미경 H-7100 (Hitachi Ltd, Japan)를 이용해 100 kV의 전압으로 관찰하였다.

그 결과, 파지 머리의 길이(length)와 너비(width)는 각각 69.31±6.17 nm와 61.46±8.84 nm로 확인되었고 파지 꼬리의 길이(length)와 너비(width)는 각각 225.23±15.19 nm와 13.77±1.78 nm였다. 그러므로 이 박테리오파지는 시포비리대(Siphoviridae)에 속하였다(도 2).

< 실시예 4> 박테리오파지 KFS -ST의 단백질 패턴 분석

4.32×10¹¹ PFU/mL 농도의 정제된 박테리오파지 KFS-ST 용액 80 μl와 5× SDS 시료 용액 20 μl를 혼합하여 5분간 가열 후, 10% SDS-PAGE를 수행하였다.

그 결과, 박테리오파지 KFS-ST를 대상으로 수행한 SDS-PAGE 결과를 나타내는 전기영동 사진에서 보이는 것과 같이, 90 kDa에서 39 kDa 사이 범위에서 단백질 밴드가 확인되었다. 2개의 주요 밴드가 생성되었으며, 그 크기는 약 41 kDa 및 39 kDa로 확인되었다(도 7).

< 실시예 5> 박테리오파지 KFS -ST의 전체 유전자 분석

박테리오파지 KFS-ST의 전체 유전자를 분석하기 위해, 박테리오파지의 유전체를 Phage DNA Isolation Kit (NORGEN Ltd, Canada)를 사용하여 분리하였다. 분리된 박테리오파지의 전체 유전자 분석은 454 및 Illumina sequencing platforms의 혼합 방법과 CodonCode Aligner 3.7.1에 의하여 수행하였다. 전체 유전자의 크기를 전기영동으로 확인하였다.

그 결과, 서열번호 1 내지 63에 나타낸 바와 같이 박테리오파지 KFS-ST의 전체 유전자 분석 결과, 이중가닥 DNA로 크기가 47 kbp이며(도 8), 84개의 ORF(open reading frame)가 확인되었다. 박테리오파지 KFS-ST의 전체 유전자 서열을 서열번호 1 내지 63로 나타내었다.

< 실시예 6> 박테리오파지 KFS -ST의 챌린지 어세이 분석

살모넬라 타이피뮤리움을 TSB 100 ml에 접종하고 190 rpm으로 37℃에서 3시간 동안 배양시켰다. 배양액의 흡광도가 640 nm에서 약 0.15에 도달하면 (10⁸ CFU/mL), KFS-ST 현탁액 1 mL을 MOI 0.01, 0.1, 1, 1, 100이 되도록 맞추고 37℃에서 110 rpm으로 24시간 동안 배양하여 박테리오파지 KFS-ST의 용균 활성을 확인하였다.

박테리오파지 KFS-ST를 접종하지 않은 살모넬라 타이피뮤리움 배양액을 대조군으로 사용하였다. 박테리오파지 KFS-ST에 의한 살모넬라 타이피뮤리움의 생장 억제 및 사멸 여부는 파장 640 nm에서의 흡광도 값을 2 시간 간격으로 측정하여 관찰하였다.

그 결과, MOI 0.01, 0.1, 1, 10, 100에서 0-2 시간 동안 박테리오파지 KFS-ST의 용균활성이 가장 활발하였다(도 9).

< 실시예 7> 박테리오파지 KFS -ST의 저장성 분석

박테리오파지 KFS-ST의 저장성 분석을 위해 100 μl의 4.32×10⁹의 박테리오파지 KFS-ST를 900 μl의 TA broth와 혼합하여 다양한 온도(-70℃, -20℃, 4℃ 및 22℃)에 저장하였다. 동결 손상을 최소화하기 위해 -70℃, -20℃에 보관하는 K박테리오파지 KFS-ST는 글리세롤의 15 %를 첨가하여 저장하였다. 2주 간격으로 용균반검사를 진행하여 -70℃, -20℃, 4℃ 및 22℃에 저장된 박테리오파지 KFS-ST의 활성을 확인하였다.

그 결과, 박테리오파지 KFS-ST의 저장성은 4℃와 22℃에서 가장 좋았으며 그 다음으로 -20°C에서 저장성이 좋게 나타났다. -20℃에서는 0주부터 6주까지 박테리오파지 KFS-ST의 농도가 0.26 log PFU/ml 감소하였으며 -70℃에서는 0주부터 6주까지 박테리오파지 KFS-ST의 농도가 0.34 log PFU/ml 감소하였다(도 10).

기탁기관명 : 한국생명공학연구원

수탁번호 : KCTC13465BP

수탁일자 : 20180122

<110> KYUNGPOOK NATIONAL UNIVERSITY <120> Novel bacteriophage having pathogen Salmonella Typhimurium specific antibacterial activity and use thereof <130> PN1712-480 <160> 63 <170> KoPatentIn 3.0 <210> 1 <211> 1117 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_1 <400> 1 agcaaccaca ccagcaatca ggacaacaac cgcaacggca acagaaacca acaaaacaac 60 aaagcccaca aggaggcaat gagcctccta tggattttga tgattcaata ccgttctgat 120 gataaaactt acccccgccg aagcggggtt ttacactata atcacacagt tgctaatgta 180 accctaacta aagaaacaat cagctttctt gacaagtgct tttagtgagc ttccttccca 240 gtatggaacg aagcaagagt tatcttttac tgcgctatcg ctaacgacgg ttgggcttaa 300 aaggtatttc tgctgccctg ttgtctgtgt agttaacctt tattgatacg gcatcactaa 360 cacttccaga cgcagccccc aaagacgccc atgccgaccc acttccagcg ccatttgaat 420 gtgccttaat actaacggca tccattgtat tgctcagctt atgcagcctg atatctaata 480 tgcccggtat ctttggtaac tgtttattcc tggaatttcc caagcatttg gttcaacaat 540 gttagcagca ttaacctttc tactgtcaac attaacaagt aatacctgat attgtagtgt 600 ttggcgcgtc tactatcagt ccgttagatg gggttggctt tataccaact aaacgcaaac 660 cattaacaat acaatctcct tgtatgtata cctgaatttg cattgaaatc cagcgtattt 720 gtatctatta ctgaaacatt ggtggaatgt ctttccatat gtaaaccata gtgctccaga 780 gcctgcgcaa tcttgcataa gacgtttgat acatagccgc cctttaccat ccattcctat 840 gccaacacct agtgagccaa tagctacaag gtcatcaatt atgtggtttg ttcggtaatt 900 gatgtactgg atactcagcc aacgtataat cacctggcct gtcagtttca ttgccgatat 960 cagcgcctaa atcaaaccca tcccacacag ggtacattga taatgatttt ctaaactgaa 1020 gattggaaac aacgggcaga tgtacttccg accgttccct gccatgtctt aacacctgac 1080 tccccaggac ggaatgacgt aaaacctata acaccgc 1117 <210> 2 <211> 269 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_2 <400> 2 tcaggaagag cggcaacagc tctaggtctt ggttggttca cgaacctcac cgcggcaatg 60 tcatcagcaa tttcaccagc aaggaccagg tggtttggta atgtcgtacc acttttcttt 120 gtttatatgt ctgccgtcag gtcataaatg gtttttatcg acatcatgag actttttgat 180 gattactttc ccatcacttg atttgcatgt gaatgatttc cctgttgcat gcccataagc 240 atgggctttt ctctgaacct ttgtatgct 269 <210> 3 <211> 7553 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_3 <400> 3 ggaaaaagaa cagggttatt cactggggcg ggctggtgtg cctgatgatg tgatttgccg 60 tcggtatgat atcgacgagg acttcctgct gcgcgctctg gattgatggt cttcgttaac 120 ctgcaagaga agcgcggata caagggtatc tgctgcaaga atgatttttt gagagggtga 180 tttgatggtt atcgaaaaaa caaatgaatg ccatagtctg tatataaata cagaacgagt 240 aatcaattta aggcaatgtg acgactgggt tcagcttgat aaaaaacaag ccgcgcagct 300 tatcgaagtc ctgcaaagct gggttaatga ttgaggagat tgagtgatga tgaataccaa 360 gcaaagatac aggatgggat attgcggttg gctgaaggag actgttggtg attttcttga 420 taagaaaatg ctggcagaca tggtttatgg cttattgtgc ctactgctac ttcatctctt 480 tccctgtttc aatgccgata gttgctgcaa taagaatgat agtaaccaag aggaatatga 540 gaaaacagta tagtcgcgat gaactgatgg gagattgagt gatgagcgtt taatttttat 600 tgctgtcaca ttgagcgaca aggaagcgtg gcgagcgtcg gtagtttctg cggattatca 660 attggccctg atgcagttat tgcatatgag gacgctatga gagcataagt gaaagaaatc 720 caggcgatca ctgaaagctg cgcttttaat ctttctgtta tttttgataa attcgaaaag 780 gtggagtgat ggctctatta tggtcgctct tgattccact taactttatg ttctggcggg 840 ctatggctgc cggagtgaat gaaccaaaga ttgattcact ttatggttgg ttcatttttc 900 gctgtggtaa ctggcgttgt gccatgtttg ctgtatcaat atttcaaata gaggtgaagt 960 gatgcacttc aggacactaa acgccatcag agatgagatg gttggtggta aatataaaaa 1020 ccacagaggc gaaatcgtaa ccgtagttaa tgttgttgcc gctggccgtg gctatcaggt 1080 tcatttttac tgcataccat acaatgttac ttgcgggctt ggaaagttca gaaagcgata 1140 cccgtaataa ggtttagaca tataccatcc atgctataat ccctccatcg tgagggattt 1200 tttattggtg aatcatgtca aaactcgacg ctttaaacgc ctatatacgc gaccgcgtgg 1260 ctaacaataa ccgggcgatt cagcaacaaa ggctttgtgc tggcgggaag aatctggacc 1320 agaagcacga cagactctgg acggaatgcg ggtacaagca ggaaattaac gcttgaggat 1380 ttccggtttg cttatgaacg ttacccgctg gcaaatgccg cagtaaatat cgttcttaac 1440 aagtcgtggc atggtatgcc tacagtgctt gagaatgacg ctgatgatga agccacttca 1500 ccgtgggaaa agagcgtcaa tgaccattct gaaaaaggcg cttccattca tcagggatgc 1560 agataagcgg aatctgatta accgatattc tgcgctaata ctgcaaatcc gtgatgggcg 1620 taagtggaat gagccggtag acgtcaccaa aactcgccgc attaaagata aatccattgt 1680 tcgctttatt ccggtgtggg gaggagcaac tccgcgtcag tgcgtggaat aatgatgaag 1740 ccaggcgaag actacggtat gcctgagatg tacgaatatc aggaaagtgc ccgttgaaga 1800 tttcgatagt gacggcaagc ctgatcgttc cgtgcagatt caccctgacc gcataattat 1860 tctggcagag ggtagctttg acggcagcat gttcagcggt gttcctttac ttcgcgctgg 1920 attcaactcc ctgatagatt gcgctaaggt ttccgggtag ctccgcagag ggattactca 1980 aaaactcatc gcgccaactg aacgtttctt tcaataaaga taacgtctca gctcagtcat 2040 tggcacagca gatgagtgtt gaagttgagg agcttgccga tctgctaaat gaaaatatag 2100 aaatgctcaa ctctggcatt gacgcggcaa tgtttaggct ttggcagtga cgtaagtgtc 2160 ctgtcaacgg caatgagcga tcctgagcca tttatgtatg ttgcagctag tcagttcgcc 2220 gcatcagtga atatccctct taactcattg cttggaagtc gaagcggcgt tcttgcatca 2280 gccaatgatg agcagtcact ggcaattgat ggctatgcaa cgacgtgatg gctggcttga 2340 ttatctggtt ggctcttttg ttgagcgttt aatcacgttt ggcattgtgg ataaggcgcc 2400 agtcgctgga tattattgca agtggaatga ccttcttgaa ccaacgcaga acgacaaggc 2460 tgaattgatt gttaaactcg ctcaggctgc acaaagtgcg gcaaatgctg gtgtcggtca 2520 aaatccttac agatgatgaa atcaggggat ttcttgggct tgagcctata gaaatgcctg 2580 atggatatat ggaagacaca cctccggaag atgaaaatga agatactccg gtttaacgct 2640 agacttccac agccgcgcat atcgcagagc cctgaatgat ccgttaggcg ctgcgactcg 2700 cctgtcaaag atggacaagg tgataacgcg caaatacaaa cagctcagga gtcgtgcact 2760 tgagttgttc cgcaccattc cgcatggtca aaccaactcc gaatcaagcg gtctgtattt 2820 ctacgatttc agtagtgcgc gcgcagcctc cttcatggat gaattgcagg cgctgattga 2880 cgagattctt cttgaaggtg atgatttcgg tcacggcagg atgtgggcca acgtgttcat 2940 tggtgatgcg tatcaggccg gaacgcaaaa ggcaaattca gagctgtcaa gcctgtctcc 3000 ggtttatgca gaacagaggc cgattgctgc gatactctac agtgagccat acctgaacag 3060 gcttcaactt gcctatgcag ctggctattc cgattggcgc ggattgagtg actattcccg 3120 ccagcaactg gcatcagtca tcatggaagg cattgcccgt ggcgctaacc ctcgcgatat 3180 tgaagatgat atcgttaagc gtgtcgatgt atctcacagc tacgcgaaac agatagcgca 3240 gactgaaatc accggaacgc ttaggcaggc taacaggcga gaagtcattg aggcgcgcga 3300 ggaattaggt attgagacgg tgatgctgtg gcagtcggca ttaatgctca caactcgcca 3360 gacacacgca gcgagacacg gacggtttta cacgccagaa gagattgaca cgttctatag 3420 cgagaatggt aataaatata attgtcactg tgcccaaatt ccaacactgt taatggatgg 3480 caaacccgtc attcttgaat cgtcacagga aagacttgat aagcagcgag aagcgtggca 3540 atccgcaaac aaaaagccct ctaagtgagg gctttgtttt atttcgatgg cttatcgcat 3600 tttctgcacc agctcgtaag gcacatgcgc ccgcaatgct tacatatagg ccacatatca 3660 atcatccagt ttcacgccgg gaattttgcc tgctgcgatg ggcttcataa atgagtttgg 3720 cctcatcctc tcgcttgtaa tcttgcgcta ccattgttaa taccgcgcag atagattcga 3780 gcacggcctc gtgcttcctt tccgcttctg tgcggagtgg tcgaatgttc atatgcctgt 3840 ggtagccgct gccttcttgc tgtgatgact tctgtttcca gacaataagt gaatcaccaa 3900 caaaaagaac ttcacactgc cctagtgatt tgccagaaaa tgatatctca cactcacacc 3960 ccactggtgg gacgccttct ccgttcccat gttggctttt gcgatgcggc gagtgctgct 4020 ttatattgcc aataggtcac tatcgactcc ctgtggtcgc aagataaagg tacgtcctca 4080 attccatgcc agtaatacac aaggctattc ccagtgtgat gtttccatga aaatccatca 4140 tagtgtggat tattcgtgtc ccatacacac agcaaaccgt catcatcttg agtaattgac 4200 agagcattaa tgaggccacc cgccacgctt aggtaattct tgaactaaaa ggtcgataag 4260 tttcatttca ttctcccagt gctttgttga ttgcatcctg tgctgattta agctcttctg 4320 ggtatgcatc attccagttt tgcttatacg ctttgctgag cattttctgc aaagcctcca 4380 gaagctcagg cgctgcggcg atgaggttgg cattatcctt atcggtaaca tcgcacacga 4440 attggccgcc tttactatag accccaccaa gttcatccat cgtaatgcca cgaccccagg 4500 gttcctttaa acttctttca tgcaacctca cttaatgtac tcaataatct caacttcgcg 4560 catctgcacc agcccaaatg gcgcaaccac tttaccgcac ggacggattt tgatttgttc 4620 aaggttgaat actccgcagc gtccgttttc tttgagtttg accattacca ttttgcactc 4680 atccatttca tttctttgac catgcgttga gctgctttct tggtgtgacc ggcagccatc 4740 aattgcttca cctttgcttt tttgttggca aacctttgca gccttcggat ttgactcact 4800 gtaaaacgta acatcacgtg gattcatttt tattacctca cgcatcattt gattgtagat 4860 actatgcacc accgctcaat ctacgtcaat acctgttgtg atagaattaa tcattatttg 4920 aacaggaggc agaatgaaac tatcggagcg cgccgcggag gtgcttggaa tcattgatgc 4980 tgtggacata tctccttaca tcaccactga gacaacgcag aatcagttcg acgcgctaac 5040 aagccttgcc gccgatatcg gcattgacgt gttccgtaaa tcagcgcttt ctgaagaaaa 5100 cacaatctcc gctgtttctc atgcgtcgtc gcgcatttca tcgtgtgggg cgagaagaca 5160 gacaacaaag caaaacgcaa ggctgaaaaa gaggtttact ggtatggcta ttagtaaaaa 5220 catgaaggcg tttctggata tgctggcgta cagcgaggga acggataatg ggcggcagaa 5280 aaccaataat cacggctatg atgtgattgt tggtggctca ctgtttgccg actattccga 5340 tcacccgcga aagctgatta gcctgcctaa gctggggtat caaatccact gccgccgggc 5400 gctatcaggt gctggctaag ttctatgatg catacaaaaa gcaactgcgt ttgcctgact 5460 tctcccccgc atcacaggac gctattgcaa tgcagctaat ccacgaatgc aaggccactg 5520 ccgatattga ggctggtcgc gtcgctgatg ccatccataa atgccgttcc cgctgggctt 5580 cattgccggg cgctggctat ggtcagcacg agcagaaact ggataagctg attcaggtgt 5640 acaaggatgc tggcggagtt gtggcatgaa aaagctaagc aactggctgc tcggcgcgtg 5700 gatttcgctc tgctcgctgt tgcagctatg gcctgacgca atgatgcatg tgtgggtaat 5760 gatgccggac gacctgaagg cggcactacc gccaatcgtg gttaaagggg tgagctactc 5820 aatcatgctg gttggtatcc tcggcaaaat gcaggcatga ggaaggaaaa ccggaggctg 5880 cgcaatgata aataatgcat ggattgcact tgcctcccga cttcacacca gatagcatca 5940 agagccacca gtggagaatg tgggagtaca gaacgcttta tcttggcatc aggaaaaaat 6000 acggcattca atggtcatat gaactgatgc gtatatttct tgataactat cagttatttc 6060 agaaggctgc gcaatgatgt cgattctcgc taaatactgg cgaccgctgg caattattat 6120 aattgttgcc gctggcgcgc tatggattcg cggagagctt atagaatacg gcgaccaacg 6180 gtacgcagaa ggaaaagctc aagcaatcac cgaacagaag gccgcagacg aacaagagga 6240 gcaacgacgc aatgcagaac tgcaaaagat tcaggccgac gcacagcaaa ggattgatgc 6300 tgcgcgcaat gatgctgtca atgctgctgc taagtctggc aggttgcagc aacaactcgc 6360 aaatatccgc aagcagctcg tcggatattc caccgctgag tccattggca atccagccgc 6420 agataccgga gttttgcttg cccaattgct ctcagaatct gtcgaaagaa atcgacaact 6480 ggcagattat gctgatagag caagagaagc tggattagca tgtgaggcgc agtacaattc 6540 gttacgcaac aaaaaagccc cgtgaagggg cttgtgttta atttgtttgc tctgagtttt 6600 ctcgttgctt ccacatctgc ttcagctgaa aataagcata cgcctcatca ccatctttcg 6660 cattgcgttc catttgctca cacccatgca gatggttttg gtggctcact ctgctggcaa 6720 tgttgttggc tcatcaacag attcctcaac cgcagcttca acgatgcgca ccagtgaaca 6780 ctcaaggtta ggtgcattgt aattgccacc cagaaacaga acgctaccag tgctaatcag 6840 cacctgcttt gcgtttgcct gagaaagatt ctgcacgatg aattgcgctg tttccgtgcc 6900 gattttcagt accgcactgc cgtcgctgtt caatgatacc agttgcgcgg aagtgtcagc 6960 aatgttggtg tagttcgcgt ttcgttgcgg cgattgtgat gtcgcagaat gacattattt 7020 aatctccagt tcttttgcgc gagttgcgct gtcaatgatg tcctgtaaat cttgttcgcg 7080 tgaatttgtg ccctcgatcg ccagcctgca acgccttttt aatcaggtgc tgcaatgccg 7140 ggttggtaac attccacgcc atgagcacat cgtacacatc aacaaatata cctggcttaa 7200 tctcgtgcag gtatttgttg tatttaacag tctcaactac atcaatgtca ttcaggttca 7260 tttcttttta aatccccatg attgctgtga aatcctaaat ttaattctgc tgattttctg 7320 catcttacag cctcatcaaa atcgacaaaa taacctaggc atgtatactt tccgtcaaac 7380 attatctgtg ctacccactt cctttggtgc ttatgccagc acacaccggt cactccacta 7440 gtgttgtatc tgtgctttga catgttgcgg gtattttccg catgactagc aagacgaaga 7500 ttttcaattc tgttgtcatc acggatgtgg tttatgcggt caatttccat tcc 7553 <210> 4 <211> 753 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_4 <400> 4 cggtgagaaa aaaccgccaa tcctgaaaca atagcagaat tacgagacct gctgttgaac 60 cttagtcaga aatatggata tgcaaacaag tctctatctg aactgactgg caaggttggc 120 gaatattcca tttcggcaga tcaggcagca gaatctacaa agatggctac tgatatgctg 180 aacgggcaca aagtagcttc agaagaggat gcgagagcca ctgaagaaaa cacaaaaagg 240 cttcaggctt atatccagtc aatcaaggag gaaggggaaa caattgcctt caccgccaga 300 caaaaagcga tatacagggc agagcagctt ggggcaagtg atgcggataa gcagacaatc 360 aatgcgtcat tcgacaaaat agaggcttac aaagctgaac ttgccgcaaa aaaagagcag 420 gaaaagcaag ataaaaaacg catagctgca tcgacaaaac aagcaaacct tgaggaatcg 480 gtgtcacaga agctggagaa attacgccag caaaccatgc tcaatgccac gtctaccagc 540 gaactatcac gcgagcaagc aattctcaac gcgcagcaat cgcttggcaa ggcagccact 600 caggagcaaa taaaactggc tggcgagtac gctgcgaaga tttgggacca gaagaatgcg 660 ctgaaggaac aggcggaagc ggagaaggaa aagcagcgcg tagagaaatc atatcaggga 720 ttgcgtgcca ttgcgtcacc aacgactggc att 753 <210> 5 <211> 298 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_5 <400> 5 agaaatcata tcagggattg cgtgccattg cgtcaccaac gactggcatt gacagtgaat 60 accagcaacg catggctgac cttgatgcct acgccgcagc atatccgcaa aaaatcacgg 120 agattgaaca gactcgcgca gcaattgaag cgcaatatcg ccagcagaga atggatgcaa 180 tgtgggctga atggcagcaa caaagcctcg gagcgcaact gttcggcact gctcttgatt 240 cagcgatgag cacagcgtca aacagcatta ccggattact gacaggcaca atgagcgt 298 <210> 6 <211> 278 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_6 <400> 6 ggcgatgaca tgagtgaaga ccctgatggc ggggttggtc atgttcgacg agttgtcagt 60 ctggttcagt ttgacaccac aagcacagca attcgaaagc ctggctaaaa gtgaatagct 120 acccgttcat attaattatc agtgcgctct atgtgcggca cgttttagtg gagatgtatg 180 gatgaccaga aaccaacgac gccgatacac cactggcgct aaaatcttcc ttgctgttta 240 tgtgctggca ctggtagcag ctattgcggg aagtggtg 278 <210> 7 <211> 10190 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_7 <400> 7 tcccacgcac aaccgcaaac cacggttttc cacttccgtc agggtcttca accaaatcac 60 cagtgcatcc agctgtatcc agtagcttca tctgattgta cagcgccacc cacggcttgc 120 ttccgtaagc gaatgattgt gatgcgccag acggtgcgct ctggctggtg acgtagcgaa 180 ccagctgtat ttgcgccaat caggatggag gcccacagca tgatcgcatt ttgtcggcat 240 tcatcacttg gatagttcag ttcaaggcat tcactgattg atgctacaag acacagaata 300 ccggacgcat ccgttgtggt gatggtcatc ccgcgcgatg ccatctggct gaccagttct 360 tcaggtgttg gtgctgtcat ttctctttga ctccctaact ttccaccaca tttcaaacag 420 gttctttatt accagagaca gagcgccaag aattgacgct actgccgccc actctgtcag 480 gctgtgtgga atcatagcct caaaataaga ttgcgtgaca ggtgtttgtt ctgcaacttt 540 cagaccgata ccggtgccaa tagaagcgta tccggccttg tcaatcacct gtcctgtcgt 600 tccgcttata atctgctctg cgacttgcct tagtgtcccg ttcattgcgt gtctcgctga 660 tgatatgttt ccagcactta tagatttgaa tcagcgaaaa aacaatggcg accacgccaa 720 gaataatgtc caatttcgcc gccccattta aaagtgatgg aaaggatgaa cagatggatg 780 ccgataataa taaatgcgta ctgcgcatga agcggcgttt ctacaggcgt gataaattcc 840 catgcgaatg actctatcgc taccagccat tcgtaaaggc tcatcgtcag cacgcagagc 900 gccatctttg tacttttgcg cagcgcaata gccggaaaca accagaccat agactgcgca 960 aggtaataca gatattcggc gggaaatgaa tcaacaagca cccatccaag atacacagac 1020 atcaccattg ccggaatgaa caccagaagc gcaaccatgc ctgtgcaggc aaaccccaac 1080 acatacatga tcatgatggc aatgtctgcg ccgaacatta tttcttgccg cgagatggtg 1140 agcgggtgga gccgtttggc ttcactgcgc cagttttggt attgccagta gcgcgcgaac 1200 gagaaggtga attggtggaa cccatgttta aatcccctgt tgtttgatta gcatgatttt 1260 agcatattcc tgttgacgta gattgatggt gcgtttatag ttagtgacgt agaaacaaca 1320 ataaatggtt agaggtgatg agatgtttga tattgataac ccaagtgatg acaaagctaa 1380 gctgattgat gcagtcgctt gcagttttgc ttcttgccag acagaaggtg aatatcaggt 1440 tgtgaaaatg tgcattgttg agttcttctc ggcaatcaac attcctgacg atgaggctgt 1500 tgatattctg ctgagttccg ctggcaattg cgctgatgcg gacgaatgca ttgatgaact 1560 ggttgaagag tttggcggta tttttcaggg tgacgaggag tgattatgag cagtaacgca 1620 aaacattatg attattacat ggttgaaggt gctgatgtta agaatgttat tgacgcatat 1680 gatgacatcc aaaagcgccg caatgaaatt ctcggcgatg ccatgaaaaa ggttggtgct 1740 gtcgctttca ctacaacacg tagctgtggc gcggtcggag gtggcttact tgtgagcttt 1800 gtgtggagtg agcagtttga atttccgtgc caagtgacaa taaagcgtga agacttttgg 1860 gaaggtcagc gtgtaattgt ggctcgcgga aaaggaaata caaaggatgg gcgggaatac 1920 aacaaaaagc tggacgcagt gattcgcgag gcaaacgaga aattaaagtt attgccagaa 1980 tgaaaggact acattgttaa ccattatggc attgcacgca caggcattgg cgagcaatca 2040 ggtcgcggat ttggttttgc aatgctgtct actatggcgg taaacacccg caaaaggatg 2100 attgcctgat ttttgctgtg ccaaatacaa aagatgaagg tcacggacag gtaacgatac 2160 ctgataactt taagcagata acatacggtc agttttacga tattgctaat tcaggtgatg 2220 aggaataaac aatgaaaaca ctaagcaaaa tctattcaga caaagaaaca cgcaatggta 2280 tcgcggttaa caaaacctat ctcgtgccag ttgagcaaat ctatctggag ccggggtaca 2340 acatccgtga agcagatgag cagcacgtcg aatatttcgc gcagtgctgg gaatctggtc 2400 aaccattacc agcattaaca gttattcctg atgagaaagg cattcgcatt cttgatggtc 2460 agcaccgcta tctcggagca ttgcgtgcca ttgagcgtgg cgcgccaatc gttcgcatcg 2520 agtgtaagga tttcacgggc gatgaggcgg ataaaatcgc gttcatggtg tcatccagtc 2580 aagggtaagc aacttgaccc gtttgaacgc gcaaaggctt acacgcgact gaaaggcttt 2640 ggcgggacga atgaagaaat cgccaagaag gtaggtcgtt cagtatctga cgtgcaaatg 2700 catttgtcgc ttggtgatgt tccggcggaa gtaaaggcgc gcatcagtgc aggacaaatc 2760 agctatgcaa atgccgtagc ggtaacgcga gaacatggcg atgatgcggt taaagttatc 2820 gacgaagcgg tcgaagaagc caaagcacaa ggcaaggata aggtcacggc gaaggtgctg 2880 aagtcgaaaa agattaagca agtagaccgc ctgattgagt tactgaagcc agcggaccac 2940 gtaattctcc ccgctggtca tgtagtggca gaggatgagg agttcatcca gattcctgtt 3000 gctgatattc atgaggtcat ggcaattctg gagaagatgt gatgagtgaa cagccgcttg 3060 ctttcagaca aatagaaaga aaagctatta agagccataa atgctgcgag tgtggaagag 3120 aaatacacca aaggagataa atataagtat tcatcaggaa tctcatcggg aaaaagatgc 3180 tttttcatat aaacaatgct tgatatgcag caaggcattc aatgcagcat ctgaaatctc 3240 agattacgat gacatgccat gctttacggg tcttagggag tggatgggaa attacttctc 3300 tcgaaattac cctgcgcaga aaagcgtgga tagtatatgc atggacttag gtttaacaga 3360 atcggaagtt cgatacatca ttccagctaa gtggttggtc ctatgaacgc tgaacaattc 3420 atcgaaaaac aacttcgcac caagctacct gacattgaac cagatggcaa ttgatgcggc 3480 aattcagtat tacaagcgca ataagagcgc aaagaagggt ggcatttttg aagaatgcct 3540 gaaggttgca aaacagcata tgattaaggt gaagtgatga aactaaaaat cagcaaatta 3600 ttacttgagt cagcattaat atttcaggcg cgcaatgata tgcgctacta cctgaatggc 3660 atttgcttta tgcaagatgg tcgtgtttca tcaactgacg gtcatcgcgc attcattggt 3720 ggcagtcatg acaatacgct gacagaaaat gtgattatca agattggcaa atctccaaca 3780 aaacgctatg actacgccat cattgatacc aagtcgaaaa ttgcaacgta tcatgatgaa 3840 gctggcgaga tggttggtgc tggtatctgc gaagagattg atggtcgatt ccctgatatt 3900 gatcgcgtca ttccgaagca gaccttacca gcagaggaaa ttggattcaa cgctggatat 3960 attgttgata ttgagaaagt ggcgaagctg tttaatccta aattctgcgg tgtccaaatt 4020 tgagcttaat ggaaatacaa atgccgcagt ttgctgtctt agtgcgccat ctggagatac 4080 tgcaaagatt gttgttatgc ctatgcgcct gtagcaataa aaagccccgt catggggctt 4140 ttcttttatt ttgcatccat ggaagccaca ataaaaaacc cgcaattatg cgggttcagt 4200 ttactctgct ttctttttct ttatcgtctt ttcttgcggg gttgcgactt cgaaagactt 4260 ttccagctct ggcatgattc gcaggttttg gtagcagatg ctcatctggt tcggtaatca 4320 cctcgccaag ctgcaactca cgaatcttgc ctttctcttt aacaaagatt ccgcgtgcga 4380 tcacttcgta tttagccatt attcacctca aattcacaaa ggggccgaag cccctatctt 4440 tattacttcg caggttgcgt gccgtatccg ttgaacactt tggacttacc ggcgaaatcc 4500 ttgcgcacct gtaatcccat agctgaccac actaggaagt tgaagttatc gtgagggttt 4560 acgcgggccg ccgcataggt ggagacaggc tgagcaacac gcggacggat gtacatatcg 4620 ttgcgaacgt agccaacgaa atgggttacc agtcagcagg aagttggtgc caatctttcc 4680 gatgcgaccg tttcccgaac tgcgtgatgt actgctcaac tgtgccgcct ttgaagcccg 4740 ccgcattaga atacggacgc atgaagctgc gacgcactgc cggggaaacc cacagagtca 4800 cctgctaaaa tacgttctgc gcatccagaa tagcctgaaa atcctgattg aagaatttca 4860 cgatttcgtc tggcgttgcg gtttgcaggt cgatattcag gccgcccccg gaaacgttca 4920 ggttaacctg tacagtgttc gggtggttag tgataccgta accagtgtaa acgccgttca 4980 cgttcagagt ctggtcgcca gtcagcaggt actgcgccat atcggaacgc aggttgaagg 5040 tgacgttagc ctgatcgtcc agcagtgggt cgaaaccctc agactgcatg cccagcagtt 5100 cgcgccattc tcggctgtag ccagtcttgc agattggaat cacatcgcca gtgtaatcgt 5160 agcgggttta tccaaatctt ccggctcctg accagacaga gtgcgcacaa ccttaccagc 5220 atcggaagca atgcgactga ttgccacggt tttaccgata ttgatgtttg ccgcgatacc 5280 catcaggtca gccatcatgt cctgaccagc ttcgttacgg aaaacgcggg tagtgacgtt 5340 gtccacgtcg cgccagtaat ctttcgttac cagtgcggtg gcgttcacac cgtaagtttt 5400 cgccagctca gcttctgcgt tgcagaacac cttacggtcg atggtcagat gtttccactg 5460 gtcagccact actgcggagt tggctaccag gtctttggta aaaataatct tttccattat 5520 taacctccag caggcatgta agcattgcca gcgcgacgaa ctgcaaccag ctcagcgcca 5580 tcagaggcaa cagtataagt ttcataggag taaaacagga tattatcccc atcaccagca 5640 actttcagcg cgccagcgcc gttgcttgcc agcggagtgc ctttcttcag cgcagaagat 5700 gcagcaacca gtgcgtgata ggtgacgcca aattcacact gtacagccat gccagtagca 5760 ttagccggaa cagcctcaga cacatcgcca ccaccgaggt agttatgctg aagcacataa 5820 gggaaaccct gaccgccagc ggtagcgtgc gcgatgattt tgtcgtcagc agtatcgaag 5880 tcaaccagtg cgcccggctt caacgcgacc ttcatgatgc cttcgcgaat ctgcgggtcg 5940 ttcttgcgag ctgggccacc aatgatggtg ccataacgga tagtagccat tattcaggtg 6000 cctccatatc aaaatcgtca tcagcgtggt ttggctggaa gccacccttc agcgcggcag 6060 gcttgctggt cagcgcatag gtttcacgca gtgcttcgcc tttcagcgca ttcacagcgg 6120 attccggcag ttttagttca gcgatgatag cagcacgcat cgcggtttct tcctgctcgg 6180 tgttcgcctg caattgctct ttcagtttaa cgttttcagc ttcgatgtcg gtcagcttct 6240 ggttgacagc tgtcaacgat tcctgaaccg gcttgagggc ttcagcgagt accgcctgta 6300 attcctcgtt cgtcattgag atttcccctt cagttgattt taccggctca agttcagtct 6360 tatcaaacag ccttgacccg ctcaccgacc aattttacca catcatcttc aacgatgtag 6420 aactgctgaa aaatctggcc tttgatttcg aatccgacac ggtcgtcgta cacagccacg 6480 atataaggcc agacatcctc accgacttca gccttcagaa tctggcgaat ctgttcgctg 6540 atgttctcaa acgacaggtc tgatttgttg gtgatgtagt cgatagcctt atgtagccac 6600 gatttataat taatcttgtt ggcgctttcg tctggcacgg ttgaatcctc aaggtttacg 6660 gtgatgcgtt cgatttgctc gccattggtg gcaaagatgc caacaccatc ttctggcgtt 6720 ccggctccct gcactcccgg tggcaggatg gcgaggtggt cccattccat gttgcgcgca 6780 atccatgagt attttttgcc tttgctcgtg ccttccgcag cttcgcggtt gagcaataag 6840 ccagttgata catggattgg ttcagcacct tcagctgagt tcatcagcga ctcaatgcga 6900 ccgagcaact cctgaccttt ctctgagcgt tcagcaataa ctttgtttac tttaaggtca 6960 acaagcacct tgccaaccat catgagaagc attctctatc catgcgccaa catgatactg 7020 gtttaccgcc cgaataatgt gcgcagaaac gaaagagcca tcaaccattg ggtggttgta 7080 tggtgctggg tttccattta gcccatgata actattgcgt atctcttacg ccgggtacag 7140 gccgccgttc atcactatgt cgtcgcatag aggggtgaca ttttttataa cgtagtgcaa 7200 gtcgccatca atcacctgct cactgatatt gctcgcggaa ttgatagtgg tgagcacgtt 7260 aacttgcaat ctattcatcg cgtagctcca cggttagttc gtggtcgaat tataacacag 7320 ggagatatag caccatgagg cggcagccag tgcaaataat ggcgaattgg tgagtgcgta 7380 gaacgtgaga aagaaagtga tagggattat gttcatgggc tacctcctga ttaaatgata 7440 gtagcccatg atgaacaaaa ggtatacgga gttagtctga aatcacatca tccataaatg 7500 cgagcgcggc acatgaaagg cagagcaggg cgccatagca aatcatctgg tacagattgt 7560 ccgcctcaaa cacccttgcg aatgcgtagg ctgataatat ccacagtaat ggaatcattt 7620 acataccctc attattttgc gactatgaaa tacgtgagat gaagcctctc taactggcat 7680 tgtccccact ggcgatttct cgcaacgaat acactttcta gtccaatatt tttggccagg 7740 gttaaatcta taccaatcct ttgactcacc acacctgtag cactgataca tgcgcatcat 7800 accaacccag cctcctgttt tgcccggtga acgtatgaca taaacttgcc aatcggcatt 7860 tccttgcgga tttgcgccag aatcgcgcca tgcaacatcc tctcctcgcc aaaatacagc 7920 ttatcgagtc gcggttttga tgagtgctcg cgtgcgcttc ataaggtcgc gtgccttaag 7980 tgctttctca tgccagacgc gatcattctt cttatcggca tattgcagtt gacgctcaac 8040 ggtttcgatt tcaaacgcca gttgcatgtc ataatcttca agctgaatga tgtctgcttt 8100 cataatgtcg tttagttgga taatcatttt ttcaccttta atcctgcttg tttgattgct 8160 tcaataactt catctgcata gtaaataacg tgacatccaa gactctctac ctctggcaat 8220 tcaatctcaa tagccgtgcg tgatgcatgc catgccatcc aatacatctc gaccatattc 8280 acatatctgt tatcctttgg gtcgcatccg gtgtattcat gaaaccattc ctcaagctgc 8340 tctttacttg tcattttcac ttcctccact atccagccca tctttctacg gttattggaa 8400 ataaactcat ccgatgttac aaacagagtt ctgccagatt tatgcttaat agcccatctc 8460 atttagtaca tcctcagcaa gtttgcggaa catgatttgc actctcgcca ccttgcgcca 8520 ctctgcctcg gtgagaatta catcctcatg ctgcggcact ccagaaatct cacaaggcgg 8580 aagtggctca tactcttgtt tcttgcgttt cgctttcccc atgatgacct cttgcgttgc 8640 gaagaagtga attaaacaga tcgctaacac taacttcctg ttcatgccat gagtaacgcc 8700 atagcttctt gtggtggtca tagcttcgcg tcacatcaca gttatgaaac ataatgctga 8760 tgcggctttt cacgatgcga taatcaatgc cagtggtggc gctgatttct ttcacctcag 8820 tgccagcgtt atcaagcaaa ttggcactta attgcatcat caatacctgc cgcatcctca 8880 gagatgaaat acttgtactg ccacttatca ccacgaatca ctgacttctc acggcgaata 8940 aagccaagtg caagcatctc atgcaaacga tgcgttgtgg tgcttgagtg ctttcctccg 9000 caatgctttt caatgtatgc gcgcgttgct cctgggtgat taataatcac gcgcatgatt 9060 tgtgatttat agtacatagt cacgctcctc tgctgcctga ctgaaatctt cggctgtgta 9120 taggtgcccg ttgcgtgtat tccatgactt cactgctgat tcttgatgga gccactcatc 9180 agtactgcac ccacactgcg agcaagcaac gtaatagcca tgaatgtttt tgtcattgta 9240 tgcgtcattg ctgccacaga atgggcactc cagcagtcct tcatcattca tcatcggcat 9300 atgtggtgct gtcattttgt catctcctta actaatttct tcatgtttaa gtcgcacaat 9360 cttcgcagaa tcctgtcgcg tcggtaatgt ttcttgctgg ttttaaacat tgctttcaaa 9420 aagccaatgc cgccatacca attcttttga ttcctgcttt tcttgtttct ggcgttggat 9480 tccctgatat cagccataac taattggtgt atcgtgctgc atttgctcat ttcacccact 9540 ccccaagatt attaaattta ggcgtatcgc ccgaccagtc gataaagtcc ttctgctggc 9600 tgcgcttacg ttgcagtcgg ttacgcactt ctcgcaactc gcactctatc cactcacgag 9660 tgcgctcaac ttcattcagt cgctggatta acgattcttc gtacagttca tctgttgtca 9720 ttggatgttc ctcaaaattt accagttgtt gccattctca gtagctcaag tacctcggtt 9780 gagtaatcga cctgcccctg atgataccct tcaaggatgg cggtttttga tatgcacaag 9840 acacagttca atgtcttcaa tatttactgc tcgcgcctcc aagcatggag cgcaaatact 9900 cacatctttc tgttattact ttttccacgc tcatttccac atcacctttt tcgttgtggt 9960 acaatctacg tcaataagta ttgactaagt gatgtagatt ggtcaacaat aattttcgag 10020 aaggagtagt aaatatgcca agaccacgac gagagccgat ggacattatc accagcattg 10080 tggagaagcg gcagccgctg acactccgcg atgttcgcta ctttgcccgt tgctatgtgg 10140 cacttgctga tatgcctaaa gatgagatgt ataaccttat cagggacaac 10190 <210> 8 <211> 2357 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_8 <400> 8 atgacatcat taaaagagct cgctctcact caggaaagac ttaagcatct actgcattac 60 aatccagata ctggtgtttt cacatgggtt cagcgcgcct caaaaagcgt aagagttggt 120 aattcaagct ggcgtaagaa taagtcaggc tatatagata tacgcatcga taagtcttta 180 cacaaagctc atcggcttgc gtggctttac atttatgtgt gtggcccaat gggaaaatag 240 atcatatcaa taacgtgaag acagataaca gaatctgcaa cctgagagaa gcaagtaata 300 atgaaaacgg atggaatgtt ggtaaaccat cgacaaatac gtcaggagta aaaggtgtga 360 gttgggatgc agagaaaaat aaatggaagg cgcattgcag gggtttttgg gaaaaaaata 420 tacggtaggt aggtttttca tcacaagaag atgctgagag agctgtggcg gagttcagag 480 agaaacatca cggggagttt tgtaatcatg gctagaaaaa gactctcaag tgtggcaatc 540 gaaaaacttg aacaggtagt tggcaactca actgcaaagc ctgaatcagc agtttttggg 600 cttgtagata agctacttca ggatggttct cccaatgtcg taaagcgcct taagatgaca 660 gccactggtg tatctgaaac tgatgaagag ccaacaatat taattcctga gcggatggag 720 cttatccttt acccgcggcg ctttaaggtg ttttatggtg gtaggggcag ttccaagagc 780 cgaacgtgta tatcctacct tatcgaaaag gcaaggttcc gcaacagtcg cgttggttgt 840 tttcgtgaga tacagaactc aatcaaagaa tcaagctatg cagagctggt cgatgagata 900 aacaggaagg gccacactca ggaataccgt tgcgttgatg gtgagataac gcaccattca 960 acgaggtcaa agtttgtttt tcgcggcctg tggcgaaaca taaccgccat caaaggtatt 1020 ggctggtctt acagatgtat tctgtgaaga atcagaaaat atcagtcagg tgtcatggga 1080 tacattaatc ccgacagtcc gtgcggctgg ctctgaaatt attattgttt ttaacccgaa 1140 taaagagact gacccaacgt ggactaactt tgttgagcct tacatcgaca agatggtcga 1200 tggcatatat caggattgat gacattgttg ttgttaatgt taactacgtc cacaacccgt 1260 ggttcactga agaacttaaa cagcacatga accagatgaa ggcagtggat tatgatcgct 1320 atctgtgggt gtatgaaggg ttattcaaca agcgttccga agaggccgtg cttggcggga 1380 aatggcaaac gctggacttc gagccatctc ccgaatgggg agggccatat tatggcattg 1440 acttttggtt tttctcagga tgcaacccgc agcaacagaa tcatatgtag aagatttggg 1500 cgatggacgc agaaacctgt atatctatcg tgactttgca aaggttggcc ttgagataac 1560 tgacacgccg gaagcgatga gatccagcat ttccaaattc agagaagtac agatggtatg 1620 gggattgcgc aaggcctgaa acaataagcc atatacgccg atctggattc gacattcatc 1680 cgtgcgcaaa atggccgggg agcattgagg acggtattac gtggctgcgt gggtgtgaac 1740 aggatttaac gtacactcaa gatgcaagaa cgtcattgaa gagatgacaa tgtacagcta 1800 caaggtggat aaattaacag ggaacatact cccagacata gttgataaat acaatcacgc 1860 gattgacagt ttgcgctatg gccttggtga ccacatcgtt cagcgcggta gcgggatgtt 1920 aatccggcgc aggcgataaa aacaaagccc tcaatcgagg gctttgtttc atttaatcaa 1980 cgctcggcga ccaagttcag caatccattc cgctgcatcc tctattttgt tgaactcata 2040 aagatgcttg ttgtcctcca taacataaaa caggatgcga ccaaatttat ttttctttgc 2100 gttaaactta aaacatcttt catatttcac tcctttcgtt tcatgagttc aatctacacc 2160 agcatcatat ctatgtcaac atttattatt aatgtttata aatctgcgcc accacacttg 2220 accaatctac accgagcgca tagtataatc tacatcaggc ttaaaggaag atatcactat 2280 gcgcagctat gcaggattta cacaggagga aaaagaacag gtttattcac tggcgcgggc 2340 tggtgtgcct gatgatg 2357 <210> 9 <211> 1528 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_9 <400> 9 tttcccatat gatgcggtgc gctaaatgct tagacttacc aatcttaacc ctaatgtatc 60 cattatcatc aactgttcca gcttttgtca tggccttaat tctgttattc ccggctggtg 120 cagaccagta cagttcacca tttatatacg tgaataatga atttaaattc atgagattac 180 ctttatattg cttgatttct atgtgaaatt tttactgcga attgcttagg ggattgattt 240 gtggtttttt agtttagatg gcacaccatg caggattcga acctgcaacc aatagcttag 300 aaggctactg ctctttccat tgagctaatg gtgtttaatt tggtggacac ggacggattt 360 gaaccttcaa tcatccgatt atgagtcggt tgctttaacc aattaagcct acgcgtccat 420 aagtgctggt ttaagcattg ccagcgtgct ttcttaatat ccagccccgt aacccataca 480 tacccctaca tatgattgcg ataatgctgg atattaagtg ttgtggagtg gcgcaccatc 540 tcactcttaa atgtcaaccc attcccagcc acgcaacaca tgctacagtt gagattatcg 600 cgcgtgttgt atggtcttgc tggtcacaac ggaaagagca cctcttagat tgctttcgca 660 tcaacttctt ggcgagatgc tctttcctgt tgtgttccct gacttattaa tcacacctca 720 gggacgcagt gcgccgaatt tgcttacaag gaatcggaat accttgctga cttacaggct 780 attacgccgc catcagaaca acatcatcgt ttgcatttat ctttgtggtc agtttctaaa 840 aagtccgcaa agtcgctcac gaaaaccatc ttgaaataca atctacacca caaaataatc 900 actgtcaaca cctgtgatat aatcaccacg ttagcagcta acacaattcg gagatcgaaa 960 tggctatttg tgcaaatgat aaaggcgttc tggtcggtcg catgacccga ctgttccttg 1020 ctgaggggtg cggtgacgca gttcctgatg cagaagactg gaagtattta ggttaaccac 1080 cagcaaaggt gttgactact cgccgcagac cactacgtcg gaagcggata ccgctggcgg 1140 atttgtttcc actctcgtta ccagctctga tatgaccatc agcgcagagg ttgaaatccg 1200 caagaatgac ccgagcgatg aatttggctt ccatcgcctg gttgagattt acgccactga 1260 actgaaagcg cgtcgccagc cttccttgtg ggtgcgcgag gtgactggtg ctactatcgt 1320 taccgcgtat tgcaacatca ccagcatcag ttatgaaggt ggcacgaacg acatcgttac 1380 tggtagcctt gagttcaagg tttacgattc tgacagcgtt accgtagaaa gccttgagcc 1440 tctggcattc actaccgacc tgcaatcaac tggcagcact ggcagcccgt taactgttgc 1500 tgttgaaggc ggagttgctc cttacact 1528 <210> 10 <211> 3150 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_11 <400> 10 caggtctcgt aattctgcta ttgtttcagg attggcgttt tttctcaccg ccgccagagc 60 ttcaaccagc ttcaaagcat cctcggcgct tatgccaaac tcatctgcca gactttttac 120 cacctgattt aagctggttt agtccgagaa tattaccctc atatgtcccg ccaagattct 180 tgagtgctgt gtttacatca attcctcttg ctttcagtgt gtcaagctgg ctgatggcgg 240 cggatagtgg cgctttccat gagccaagtg cgtcgccaag ctcattgacg ccctgtactg 300 aagccttgat gatgttgttt gcatcaatta gagccattgt taactgtgct cttgcggcgt 360 tttctgactg cttagccagt gttgcaaatt tgtcagagag aaccacaaca accattagca 420 gaaagctgga atatgtcggc aagacgctgt tgtgcggcgg tcagtgcctt tgtggaaaca 480 gctgcgttat ccatgccagc aattaatgag ccaacgatta ccgtaccgat ggttagcaat 540 gcaccggcaa ctgcgcctcc agaaccaaag acgcttaata actgactacc ttgttgactt 600 aaagcaacga gtgccgattg tccgctctgc acctgaacta caaagtcctg aatctggtag 660 ccgccctgtc tcatggcgtt atttaaactg gagaaactcc tgccagattt ctcagctgtc 720 gatgcggtgt tttttagcct tgtgtcaagt ttatttatcc cggcaattga ctcatcaatt 780 gacttctcaa tctcctgaag aaccttgttt acccttttgc ccgccagtag tcagcccgga 840 tacatccatc ccgacttcat aaacaattcc gccgacttct tcagccatta tgtattcctc 900 gcttttttcg ctttgcgttc agccagtgcc ttcatgcgct cacggtctgc tttagcctga 960 tcgtactcag ccgcacgttc ttctttcgtt aaccctttcg gccctggata tttattctta 1020 tcatcatctg aaactctgtc atggacaggg ttttcggcct catctcgcgt catgtcgaag 1080 tgcgtgcgcg ctgagatgat gtattgcgac gcatgaaact cgtttgtggt tttcttgctc 1140 tgctcttcca gacgctcagg cactttgagt ggtgacttac cgatgatgcc atgctgcatc 1200 aggttgcgcg caataataat aatgtcgttc actggcatga tgccaggcac gtatcgcacg 1260 ccacgcggag ttggcttcca tccgccaatc agcacagaaa tatcatcttc acagcatgac 1320 tgcatgacga tataggccgc actcagcaca tggcgaccat acactggctt gctgatggtt 1380 ttcatgactt gcatctgcgc accaaatggc aggcactcgc cgtgctgcag cggcgtaaca 1440 taatcaatgc cattgagctt agcgtacacc tctacagttt cttttggcgt gccgatggag 1500 ttcatggcgc ggaatgatgg cttaaagaaa aaactcctgt cggaaagcga gatgcgcatc 1560 tctccgattt ctgttagtgg cgtgcgattg ctcatgtttt gcatcctgaa tttgactgat 1620 gttgattata tcatctcagt ggtgttgaca cctgctaggt ggtgatgtag attcaagtca 1680 tcgaaacgag tgatgaataa atgaggtgag ttatgaaaat taaaacggtg gattgcatca 1740 gtgctatttg cttcctcctt tgcggtaagc gcaacctctg aggtttgcaa aaacatcgga 1800 gaaatcgcca tgaacactgc cgatgctcgc gataacggta ttagcaagaa tcttgccgag 1860 gtggtagtaa aaggctcagc aaaaaacaac gaatcagcag aaataatcgg ccttgctatc 1920 gtagaaatgg gtttatgcac gcaaggatat gactaaagag caattgcgtg atgtggctgt 1980 tgctttgtgc gaaaagaatg gcatgtaata taaacaagtt attaacagtt gaggttaggt 2040 tatggtcgtt gttattttgt tggttttttg ttagggttgg gtgatatttg cgtacctcat 2100 gccatctttt gtggctctgc aacgcaagca cgttaaccac gactgcaatc tgcgtactga 2160 ttattcttgt cggatggagt tttattggct gggttgcagc acttgtctgc gcattagtta 2220 acagttggcg acaagaaatg aacgaacaaa caaaagctga cctgattttc tacactgaac 2280 tgtatgtcga tgcagggtac gactatgaag aagctgaacg catggcgaaa gatttgcttc 2340 gtgtgattgg tgtgattttt gatgaggata cggtgatatg aaaaattatg aggacatgag 2400 tgattcacaa atagcgcaaa aggttttctt ttttgtgagt ggtaatcttt gccctaacgg 2460 agggttagct catatcagtt cagatggctt tttctttttt gatgaaaaga acattaagag 2520 aaagtttgac ccatgcaaca acccatatga tgcgtggcct attattgttg aaaaccatat 2580 cgcagtcgta ccatatcaat acaccatccc acaggcttgg cccacggcat ttggcgcggc 2640 ttcaaagttc accactgaag acggaaaccc actccgcgct gccatgattg tctttctgaa 2700 aatgaatgag ggtaaattat gagtcagtgg attaagtgta gtgagcggat gccggaagaa 2760 ggcaaaatgg ttctcacggc gtttcgtggc gtggttagaa cagcaatatg caaaacagcc 2820 gatgggaatg gaagccagga tatttgttag tgaattagac cgctgccacg gaattccaga 2880 tacccatttg ggccaatcca ccgcaaacca ccacaagaat aaaacaaagc ccctttcggg 2940 gctttttctt tgtcaggata cggtgcacgc cttgctgaca ataatttccg ggtcagtaga 3000 tgaatccgtg actgtgacgg tatatacacc agcggtaggg cttgccagtg tcgcgccaga 3060 cttaacaacc aacaaccgcg ccatctttgc gccatacata agtgtaagga gcaactccgc 3120 cttcaacagc aacagttaac gggctgccag 3150 <210> 11 <211> 210 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_11 <400> 11 tatgtaaaca gcaagcgtcg gtagcggaaa gtcactgatg aatggccttg agccaaggcg 60 ttttcaggaa attggctatt cagggatgaa ttttatcaag acagggcgaa atagtggagc 120 aagatgcgga agagctatgg tcgcttggtg taagaaactc gctattcagc gcttcgttgg 180 ggaggaaaag ttctacgtat ccaataattt 210 <210> 12 <211> 733 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_12 <400> 12 taccatgccg catgatgtgg tgttcaaact gaatatcact tacgcgcagg gaggtggtgc 60 atctgtaact gaaaacataa caatgtctgg tcgtcttata tctgcaacag aaacagacga 120 tggagggttt accgccaacc caatactggt acacatttat tataaataat attcaaacta 180 ctcaggcgcg ccgatatcat ctctaaatgg cgtgacgatt aataatacct atttcaacct 240 gacagacaac cagccgattg tttctggtcc gtatttctcg cccaattgaa ggtgatcagc 300 tttgggttca cctgcaacac cagaccaatg atggcaatga tttcagcgta ctcattgagt 360 ggtggaagat tgacgatgat aacgttcaga ttcccgggac atatcagtcg atgaactatt 420 atcaggacgt ggacagaaat gatacgttct actacacgat caagttaaca ccatcctgct 480 ggtactggtc gctacgcgat ccagatgcga cggacaaaca acagttccga cacttcaatt 540 cttcagcttg aggaggttca ctcaatcgtc acgagaacaa acgtctcata tccagatgac 600 actgtggtta aggttgttgt gcgcgccaca gagaacgcaa caggcagccg tgacaggaaa 660 tataatgcgc taatcacgcg ccacaccatc ggatacaacc gtgatactgg aacggtgcgc 720 tacacgcttg cac 733 <210> 13 <211> 655 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_13 <400> 13 taatgatggc attcgtgaat cctcggttac cggcggtctc ttgattggat gcggcagccc 60 ggagaccaat ccgggcaggg agctaccctt tgccgcatta attattataa atcagtttcc 120 tgttgttgct ccagcagttt ctccagtcgc tcaaagccga gcagcgaggt tcggtaactt 180 cggcaatatc aagaccagcc tttataacct cggcaaaagt cttgccaatg tccacaggta 240 taacgccagc agctatgcca cgaataacag catcaatctt ctctaccgga gttccgtcat 300 cagggaaatc aacttccaaa accaggggcg acaggcttag gtaacggact gaatcgaacg 360 ataagttctt tcatcattgc cgttgtcacg ttcaatcatc gccatctcaa cgatggtgtc 420 atagagtttt tcctcgctaa accccttcct ttcaagagct tcaacgagca ccttacgcct 480 gtcttttccg cttttatttt gttgtttgaa ttttctttac tggaatagct ttttagccat 540 agataaaacc tatcaatatt ccgctatttt tccggttatt cgatattgta acagattcta 600 caccataggg ttaatggaaa aaaagaaccc gcaacaggag ggttaaaggt tgggt 655 <210> 14 <211> 639 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_14 <400> 14 acgccgacac agagtacatt aatggctctg gtttgtggaa catttatgac cgctgtcccg 60 cagcatggcg ctacaaagac gaagaggatg agcagtcaaa tgccctcaat ttccggcaca 120 ggaagccaca cagctctgct tgaacctgaa cgtttcgatg ctgaatatgc tcgtatgcca 180 acaaaagaag attttggtga tgacctgctt gttactgcca gtgacatgaa ttcatgggca 240 aaagaacgag gaatcaaagg actttccggg gaagtcgaaa gctgaggtga tcaaaatcat 300 ccaggcgact ggcgagccag tgaaaattta cgatgtcatc cgttaaagag gctgaaaaag 360 ctgctgtagg aaagcaaatg ctggaaggtt gtgattatga cgccattcag caaatgcgtg 420 ctgtaatcca cgcagaccca tactttaaga gtgttttcac tggtggttac tctgaagttt 480 caatacttgg agaactaatg ggtgaaccat ccaaaggtta gatatgatta tttatctact 540 ggagctgatg tttgggatta caaaacagcc ataagttcaa agcctgaaga gtttttccgg 600 catgctgcgc gacttggtta ctttatgaag atggcgatg 639 <210> 15 <211> 1643 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_15 <400> 15 cggcatggta aggttcaacg aaataatcaa acgccgaatc cttcggtatc tgactgctat 60 cttggccacc agcaaattct gtctccgtga ctgtagtggt tgttgcagtc tcaataacca 120 ctggcggtgg gtcggtatcc agttcgtttg gcccgtacag ttcctgccca tccaacatca 180 tcaaaagcat aaggctcata cacaacaggg atgacctccc ctggatgata aatagtgtaa 240 cttgcaccag caagtgagcc gaggtttgac tcggagtaac gcacagaaga tacatcatac 300 ttgccgaggc caaagttcat gaactcggtc acgtacttga tattgtttac atattcaaac 360 aatgattcct gaagcaaatc agggaacgcg cgaatctgcc cgaagttatc agggcgcgcc 420 tcaccatttc gcgcaatggt tggtctgtgc tttcaggctg gtgttaggtg atgttttgga 480 gctggtgtcc tttttggcgt ggataccttc ggtgtaagga aggagaaaat cttcgttact 540 ggcttcagta tcgagctgat aaggtcgcca attgcgccgg atggctggca ataaatgttg 600 acaacgtcgc catctctcaa gcagaagaaa agctcatcat cttcaccaag cactctacca 660 tttaccgcaa ttgaaatgct ggctggcagg tttgatttat tcagccactt ccacaggtta 720 gtgcccgctg gcacaattcc cgtctctttc ggcgtttccc ggcatcttct gaacatgaat 780 tactggcata ggtgagaaac cttaactttg ttgatttttt ttgagtgttc gcagcctgtc 840 gaatctgact gccgttttct ctcgcgcatg aagtattcta tcacggcccc atatcattgc 900 cgatgtgtac cggaacgttg ccgcgatatg ccacgacaac atcgccagtt actggtgatt 960 gcgtgtcctt ccagaacgtc acctcgctat cgaaacaggt aacaaaagcg ccgccattgt 1020 cgtagctatc gtcatgatga atattgatgc cacggcacag gcggtaataa agaaccacca 1080 gcccccagca atcaacggcg tcaacatggc aacatctgtc cttgtacggc ttgccaaaca 1140 ttaactgcgc aaattcttca tcagacatta cgcagcccgg ggaattgagc gatgtcataa 1200 agttttgcca cgtttccttt gattgggttt ttgattgaca gggtcacggt aacgttaaga 1260 accatccatt gccacatcgc tgacatacag gcgatattgg cttcagtggt gtgtttgtgc 1320 cagtttcttc aaatcgctga tacagtgcgc tgattggctc aatacgaacc ggaaaccagt 1380 ccacaattta aggtattgct tgaaatcatt cgccagacgt gcaaacttga cggttgcgtt 1440 catggctgga gtgttcgact gctgagactg tgtgatgtcc atgcgcactg gcagataagt 1500 ttcaccgcca agcactatct catccagcac gttagccaca agccgcacat aaccaaaaga 1560 cgagttggta aaacgttatc gtgtcgaata acgcccagtt agggcgcttt gctttgtaat 1620 cgcgtaatga tggcataatt gat 1643 <210> 16 <211> 717 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_16 <400> 16 tcggcaatag cgagctgtag ggtcgttttt agtgccgcaa ccatcatcaa aaattctgat 60 tccgtgctta tcgaaaccgc aacggatata actgaaaaac tcttcgcagc gaccatctgg 120 cgacaatgca tctttgccaa tgcagcgacg cgcatatgga ctgttcatcg ttccgcattt 180 cgggcacgga acctgttcac cactgcgttt tgaacgttgc gcctcagcct cttcaagaat 240 cgggtccttc catacagctg accaagctca aacatcgtgc ctgagaaatc cagaaccaga 300 tggtcttctt tatgataccc cggcgtcaat ttgctctttc ttcagcaagc gcatcccgcg 360 accaagaagc tggacaagga gagttagcga cattattttt cgtaatatgc agctcgtatc 420 ccatagcgga atgttgacac cagtggtaag ggccgctatc tggaatgtga attttttgcg 480 cccggtgtat gcatctttta gagccttcct tctggctttt ggccccatat cttctgtaac 540 tattgaatag cttccttcag gtagatattt tgccgcctcc tgacagttgc ttcttgccag 600 cacatgtaat caatactccg tttctgtttt tagtaagctc catcaccttt aagcatgatt 660 ttctgtgtaa gagttccttg ctcaaggatt tctttctgca tctgcttgag ctgctcg 717 <210> 17 <211> 373 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_17 <400> 17 ctctttgctt tgttgatgta ttttagcacg gcgcaagttt caccactcca tcagtagctg 60 gtttaaaatg cattcgttcc atgatgtttt gccatctaaa atagcttgca aaaattctac 120 atttgaatgt agaatttaat tgtcacatta aacaggagaa ataatcatga aactgcaaac 180 attagagttt ccggtacgga gagatcactt tcgaccgtga aaattttatt aacgttaatg 240 agcttgttaa ccagatgaac cgctggagac tggataatgg gatggggaca agaggaaata 300 cgacaggtaa tggcaactga tgcatttaag gatttcaagg ctgagtgcga aaagcaactt 360 ggtatcgata gcg 373 <210> 18 <211> 213 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_18 <400> 18 agcattaagt tccacgaaac ttaccgcacc aatatatcgc aactcaacct gtctctgcgt 60 aatgaatcgg tcgatgacaa ggaaatggaa atatgtgctg tattgctctg ctatatatac 120 agccagatgc aagcaagcaa aagtggaacc attcctgcct cgcgttgatt ttattgcgct 180 atcgatacca agttgctttt cgcactcagc ctt 213 <210> 19 <211> 1486 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_19 <400> 19 tgcataacgg agcaatacct gacggaatgg aaattgaccg cataaaccac gacaagattg 60 ataacaggat tgagaattta agaatcgtta gcaggttaat aaatatgcaa aataagagta 120 aatacaaaaa caattcatca ggagtgtgtg gcgtaagttg gcacaaggtt aagtgtttat 180 gggtgtcgta tatccaaaaa aacaagagca gggtcatgct tggttatttt gatgatttct 240 tcgaggctgt atgtgccaga aagtctgctg agataagcct cgaatttcat agcaatcacg 300 gcaaataatt acccgtaaac tacccttaat aacaattcgt gaacaggcct cttctcttct 360 gttaacatca tatcgccaag cggagattgc atctgtatgt agttcaggca gctatcaatc 420 gggttttctt ttatgtattc aattatttcg ttagctttat tatcaacaac ttcggcatca 480 tattgccctt gctgaccaac caacaaacag cttgaaataa aagtcatttg ataacccttg 540 gcggatacct gttccgccac ttgttttcag gacaagaaag cgatcctcgc ctactccagt 600 atcattccat aattgaagtt gtgcagtcca cccatccatc agtcctgcat cgtttaaata 660 attataaact cgctcaagca tattcatagt ttcatctcct tcttaatcac gccatcaaca 720 aggtctatgg tgcgctgcgc cgctttggtc aggaattgtg tctcaccgcc cggcgaccag 780 tatgtgccat tgccgttgct gcgtggcctt cccttttagt ttgccactgg cgttatgaac 840 atacagagca taattcgcag aatagccaac cttgccagtg attcgcgttc cgttaacttc 900 cacggtatca aactgactgt taatcagcgt tgatgtatca attggcgtta gcgtggctga 960 ttcggtgcgg atgatgtatg tcgctgactt gattgcacgc acagctttcg tggcgattat 1020 ctcatccaca atttgcgaag ttctctctac agcctgccgg acacctctta atttcgctgg 1080 cattatgtca ccagtgcaaa gtctggaggc tctgccctat tgaaagtcga accgtaatta 1140 atcacactca gaatctgatt tgcgccagcc gccagttggg tcagcttctg tcgccgtgcc 1200 aatcatgatg tagtcaccta ccatagctgc agtgtattct gtccagaagg tattcttctg 1260 cacaatctca ttacctttcg catcggtcga tacatcatca ttaaagccgt aatcacacat 1320 gatgctcact ggtgcatcaa aagtcggctt gccgtacttg tcagtgccgc ttttgtgcca 1380 gatggtgcat ggctgcgtgt taactccagt tagccagtga agtcatttgc atttactccc 1440 acgcacaacc gcaaaccacg gttttccact tccgtcaggg tcttca 1486 <210> 20 <211> 763 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_20 <400> 20 aaggataggt cgaattttct cattttccgc tagcagtcca cttcgattta attcgtaaac 60 aagcagtagt aattcctgct ttaacaagat aatttttcga ctcatcagaa atatccgaaa 120 gtgttaactt tctgcgtcat ggaagcgata aaactctgca ggttggatac gccaatcatt 180 tttatcgaag cgcgcataaa aatttgagca gatttgtcgt cacaggttgc gccgccaaaa 240 cgtcggctac agtaactttt cccagcctca atctcatctc tcttttttgc gttctgcttc 300 aatatctggt tgaacggcgt cgcgtcgtaa cccagcttgg taagttggga ttaagcactc 360 cgtggacaga tttgtcattg tgagcatttt catccccgaa gttgcggctc attctgattc 420 tgaacagctt cttgggaagt agcgacagct tggtttttag tgagttgttc cattctttag 480 ctccctagac cttttagcag caaggtccat atctgacttt ttgttaacgt atttagccac 540 atagaaacca aacagccata taactggtag ctttaagcgg ctcaccttta gtatcaacag 600 gccacaacca aaccagaacg tgaaaaaagc gtcctgcgtg tagcgaactg cgatgggcat 660 actgtaacca taagggccac gtattttgca agctatttaa ctggcggcga ttgcgtaccc 720 gacgaccaaa attagggtca acgctacctg taggaagtgt cct 763 <210> 21 <211> 922 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_21 <400> 21 ggatgacata ttcgtacatg tcatcactgt attcgctcat gggtcagagt tttgtaccgt 60 cacttcctgg atttttctgg ctgacaaacc aacagagtgg tatccagttc tttttcagtg 120 ctcagcacat agcgagattc agactgcacg cttgctccat cccatatatt cagctggaaa 180 tcagtcggca ggttgcaggt gaatgtatgc aatccggtca ctgttgctgg taatctgtct 240 gatgtgctgc catcagaact tgttaatcac aacataaaga ttatcatcag ccgttagttg 300 ctcgcttgtt gtgaatacgt ttccgttgcg cgcttcaata acgccagtct gttgcacatc 360 atcatacata tcgacaacct gaatcatgtc accaacgttc acatattccc catccgaaag 420 agccttaatc tccatgctac gacgggagta aatcaggcgg cgacactcaa ggattgccct 480 gtcaacagcc tgatatcgat tgcgaacata aagcatgtcg aatttcttgg ctttcgttgg 540 ttcgccttcg actattccgc tatcggttaa ttcggtagta aacgttggct tgctaattcg 600 ttgttgggtc gcggtattcg acgttaaccc cgtcataagt ccctggaagg ctgatgtcgt 660 aacttaactt gtatccgtcg ctctgcgtgt ttctggtgtt aaatactgtt gctggatatt 720 ctcgtttctc atcacgcgaa aagctcatca cgccatcatt ccaagaatgc ggtgactcgt 780 gctgcgtcgc agattggttt gcaggcgttc gccaatactt ttatcttcat catcgaatgt 840 atagtcgaaa caaccaagtc gctcatcagg caggctgtcg gcaatttcat acagcttcac 900 tatgtcgatt gtgttttctg ga 922 <210> 22 <211> 304 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_22 <400> 22 ccttgctgtc cacattgacg atggacatga tggcagcctg ccagttgccg agcatttttg 60 gctcgatttc tttccagtcg tatctcataa aatcttcctg ttgacgatga tgttggaatc 120 attgtaattt acctacatga aacaatcaat cgttacttgc aatgaaacaa ttaatcttta 180 caatagagga taagaaatgg ctatcaccgt taagaagtgc gaggtttgcg gaaatgagtt 240 tatcggaact gcaaaagcca aattgttgct caggaaagtg tagattgcgc aagcacagac 300 agaa 304 <210> 23 <211> 206 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_23 <400> 23 ttatgacatg agagtttcac ttggtggcgt tgagtctcac gaatcgcctt ttctgctgat 60 tctatggtgt caaacaaacc aagatgaacc ctcttcttgt ttattgatgt ctgcgctctc 120 ccatttgttg catcttttat cgaaatgcac accttttaca ccagatttat ttgttgattt 180 ctttttcatg ttgaacatgt tttgtt 206 <210> 24 <211> 328 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_24 <400> 24 ttgaacatgt tttgttggtg actagaaatt cctaaggttg caaattctat ttctgatttg 60 tcaccattta tatggttcta tttcttaccc atcaatatct tctccataaa cataaatcca 120 tgcccagcct attgagcttt ccttagtttc ccctagtagc cgtgatgggt aattgtaccc 180 tgccttattc ttatgcccaa caacccttgc ctttacgcca gagtgttcca tgtgttttgg 240 cagctgtaaa gacgccagtc tcggggttat attcaagata ttccattatc tgtttttgag 300 ttatgaattg ggtttttctt ctgtctgt 328 <210> 25 <211> 467 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_25 <400> 25 cttttgatgc cgaccctaaa ttttttgcct gtttggttcg ctttgagtct tcttcggttc 60 cgactaccct cccgactgcc tatgatgttt atcctttgga tggtcgccat gatggtggtt 120 attataccgt caaggactgt gtgactattg acgtcctttc cccgtacgcc gggcaattaa 180 tggtttatgt tggtttcatg gtttggtcta actttaccgc tactaaattg ccgcggattt 240 ggtttcgctg aatcaggtta ttaaagagat tatttgtctc cagccactta agtgaggtga 300 tttatgtttg gtgctattgc tggcggtatt gcttctgctc ttgctggttg gcgccatgtc 360 taaattggtt gggaggcggt caaaaagccg cctccggtgg cattcaaggt gatgtgcttg 420 ctaccgataa caatactgta ggcatgggtg atgctggtat taaatct 467 <210> 26 <211> 207 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_26 <400> 26 ccattcaagg ctctaaatgt tcctaaccct ggatgaggcc gtccctagtt ttttctggtg 60 ctattggcta aagctggtta aaggacttct tgaaggtacg ttgcaggctt ggcacttctg 120 ccgtttctga taagttgctt gatttggttg gacctggttg gcaagtctgc cgctgataaa 180 ggaaaggata ctcgtgatta tcttgct 207 <210> 27 <211> 201 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_27 <400> 27 catggccgta cttatctttg caccttttca ttagctcatt aattatcact gcatactgcg 60 tatcaccatc attaacaaca tcaatgtcat taaccatatg attttcgtct atcagcagaa 120 atctcgggca aaagtcagaa agcgcacctt ttgcaataac attccctgat tcatcctttt 180 tatcaaacaa agcattttac a 201 <210> 28 <211> 1117 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_28 <400> 28 gtattgctta ttggttaatt cattattgaa atagactctc atttaatcac cactgtattc 60 acgtaacgct tcgacaattt tcattgcatc atcaagcaat gaatagccat cactggacaa 120 catgaccgcc atcatcaagt tcgtttaact taacaagaag attgcgtagc atttcgcatt 180 gccactcagc atcaaaacta tccggaatct caatatccat cttcatcacc tcgcttgtta 240 ttgttagttg aatctacatc aatccacatc aaaaaggcaa gccaaaatag aaaaagtgtg 300 gcttttttta gttcatcaag tccataagcg atggcagcat aatgaccaag actgcacatc 360 tcagttaaca cagccgattg ctccttgcta accctgctct tacttttgtc gcggcgtttt 420 gcctcaatca ttccgcaact gtgtttcacc cccggagtca ttatcacatt atcgcctatt 480 ccagtcttaa cccccatctt ctggcgcttc agtacaaact gcaccctgct tgatgtgcca 540 gtttcattag gcacatgaaa ccacagaaca tcagggaagc gatgttgcat ccatgttccg 600 taacccatct ggtctatctc ctctttcgga cattctccgc gataaccgct atcgaatacc 660 ccatataccg ctgtcaagcc gcttcacttt gctctccttt aaagtctttt cggtggatga 720 tgtctcgacc tttatcatta atgcgatgcg tgatgcgctt tggcgcttta atcaatcctg 780 catacatcat gaattgcttg gcgttctggc acttcaggat ttttccagcc attgatttat 840 catcaaggtg aggggaatat agcctttgcc ttgaacatgt ttttcatgtg agtcgcgccg 900 ccgtatggat agaatacttc gttagcccat ccttctttgc catcgcaacg attaatccag 960 tagcgatata aaatcccctc tccatccttc gttaactgaa ctttgaaatc cataacatca 1020 gcccactcat tatccgtata cgcacgctca ttcaacgccg cgttagggtc gcgcaaaacg 1080 tgatcgcaat gtcggcaata gcgagctgta gggtcgt 1117 <210> 29 <211> 239 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_29 <400> 29 actcaaagca ttgggattat cataaaacgc ccctataatc ggtcgtcagc acgtgagagt 60 gtcaaaaacg ataaaccaac cccatcagca tgagcctgtc gcattgcatt catcaaaaac 120 gctgaattag caaagcctct acgcgatttc atagtggagg cctccagcca atcttgaaca 180 ctcatcctta atacctttct ttttggggta attatactca tcgcgaatag ccttaagag 239 <210> 30 <211> 469 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_30 <400> 30 ccttaagagg gccgttcagc agcaagcttg cggcaaaact gcgtaaccgt ctctcgttcc 60 tctaaaaacc atttttcgtc cccttcgggg cggtggtcta tagtgttatt aattatcaag 120 ttgggggagc acattgtaag cattgtgcca attcatccat taacttctca gtaacagata 180 caaactcatc acgaacgtca gaagcagcct tatggccgtc aacatacata tcaccattat 240 cgaactcaac gccctgcata cgaaaagaca gaatctcttc caagagcttg atgcggttat 300 ccatctgctt atggaagcca agcattgggg gattgagaaa gagtagaaat gccacaagcc 360 tcaatagcag gtttaagaag cctcgatacg ctcaaagtca aaataatcag cgtgacattc 420 agaagggtaa taagaacgaa ccaataaaaa agcctccaag atttggagg 469 <210> 31 <211> 257 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_31 <400> 31 acgcgaaaag gctggatgca atcaggaaag agctaccgaa gataaatcac acgatggact 60 tcatcactga gcaggaagtc gccgcaatca tggctaaaac tacgcgtgta gcgatgaaat 120 gagtggacat tgataaatgg tattggttgc gacgttaatg tcgccagagg taaatctgga 180 gagcttattt gcggttgaga tgatgcgcgc tgacacaatg aagccgtgca acgtggcatt 240 tcattggcat ggatggt 257 <210> 32 <211> 266 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_32 <400> 32 cgtggcattc attggcatgg atggtgattc attcagagcg tttttccgcg caggatacaa 60 caaggactca accatcaacg gcaaactcac tcgcggcgcg ataagttcaa tcggagaaga 120 caatggaaag ttcatttacc tggtatctga ttatgccgat gcgtggaaat gccattactt 180 cactggtgcg catgtctggt gctgctggtc gccggaaaac atgtgggaag tggtgcgttc 240 tgttagccga tgagacgaaa gcacgg 266 <210> 33 <211> 421 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_33 <400> 33 ctccaaagat ttggaggcat gaaaacatac aattgggaag ggtgtcaatc caccggttat 60 ttcctagaca aattagagcc aatacccatc agctttaccg tctttccaga aattgttcca 120 agtatcgcgg caacagcctt tatcaatacc atgaaaaatt atcaaccaca ccagaagcag 180 catcagtgac gacattagaa atatcctttg caagtagcgc caatatgaga agagccatac 240 cgctgattct gcgtttgctg atgaactaag gtccaacctc agcactaacc ttgcgagtca 300 tttctttgat ttggtcattg gtaaaatact gaccagccgt ttgagcttga gtaagcattt 360 ggcgcataat ctcggaaacc tgctgttgcc ttggaaagat tggtgttttc cataataggc 420 g 421 <210> 34 <211> 400 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_34 <400> 34 ccaattgaag gattttaata aatggcacgg cggcgtaaac aaagtaatta tgtcggcact 60 ctcggaaacg accctggagg ttaaatatgc agcatcaggc tctgcaattg ccaacctttc 120 cgttgcaaca tctgagcagt ggaaagacaa gcaaacagga gaaaagaaag agcagactga 180 atggcatcgc gtggtcatct tcggaaaact tgcagaagtt gcaggagaat accttcgaaa 240 aggctcgcag gtttatatcg agggacagct tcgcactcgt aagtggactg acagcaacgg 300 cgtagaccga tataccactg tgatattttc attccacaga tgggcggagt tatgcagatg 360 ctttggcggt aaacgtgatg attccgggcc agcaaccaca 400 <210> 35 <211> 5764 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_35 <400> 35 ccactttccg atcgcccaaa acaaaacccc tgactgcgtt agcaatcagg ggttctggaa 60 tttaatcttg acgatgacct actctcacat ggggaaaccc cacactacca tcggcgatgc 120 atcgtttcac tgctgagttc gggatgggat caggtggttc caatgctcta tggtcgtcaa 180 gaaattcggg tactgagtcg tgacctgttg gcctcgcttc agcaaattgg gtatgtgaca 240 gctttcggtg ttttgtgaac gtcgaacttt cggttcattt cgtcttcaca caccgcaatc 300 tggtgccttt tcaggtcagc aaattgcttg ggtgttatat ggtcaagcct cacgggcaat 360 tagtattggt tagctcaacg cctcacagcg cttacacacc caacctatca acgtcgtagt 420 cttcgacggc ccttcaggga actcaaggtt ccagtgagat ctcatcttga ggctagtttc 480 ccgcttagat gctttcagcg gttatctatt ccgaacatag ctacccggca atgccactgg 540 cgtgacaacc ggaacaccag aggttcgtcc actccggtcc tctcgtacta ggagcagccc 600 ctctcaaatc tcaaacgtcc acggcagata gggaccgaac tgtctcacga cgttctaaac 660 ccagctcgcg taccacttta aatggcgaac agccataccc ttgggaccgg cttcagcccc 720 aggatgtgat gagccgacat cgaggtgcca aacaccgccg tcgatatgaa ctcttgggcg 780 gtatcagcct gttatccccg gagtaccttt tatccgttga gcgatggccc ttccatacag 840 aaccaccgga tcactaagac ctactttcgt acctgctcga cgtgtctgtc tcgcagtcaa 900 gcgcgctttt gcctttatac tctacgaccg atttccgacc ggtctgagcg caccttcgta 960 ctcctccgtt actctttagg aggagaccgc cccagtcaaa ctacccacca tacactgtcc 1020 tcgatccgga taacggacct gagttagaac ctcaaagttg ccagggtggt atttcaagga 1080 tggctccacg cagactggcg tccacgcttc aaagcctccc acctatccta cacaagcaaa 1140 ttcaaagtcc agtgcaaagc tatagtaaag gttcacgggg tctttccgtc tagccgcgga 1200 tacactgcat cttcacagcg atttcaattt cactgagtct cgggtggaga cagcgccgcc 1260 atcgttacgc cattcgtgca ggtcggaact tacccgacaa ggaatttcgc taccttagga 1320 ccgttatagt tacggccgcc gtttaccggg gcttcgatca agagcttcgc gttagctaac 1380 cccatcaatt aaccttccgg caccgggcag gcgtcacacc ctatacgtcc actttcgtgt 1440 ttgcagagtg ctgtgttttt aataaacagt cgcagcggcc tggtatcttc gaccggcatg 1500 agcttacgga gcaagtcctt caccctcacc ggcgcacctt ctcccgaagt tacggtgcca 1560 ttttgcctag ttccttcacc cgagttctct caagcgcctt ggtattctct acccaaccac 1620 ctgtgtcggt ttggggtacg gttcctggtt acctgaagct tagaagcttt tcttggaagc 1680 atggcatcaa ccacttcgtt acctaaaagg taactcgtca tcagctctcg gccttagaat 1740 cccggattta cctaagattc cagcctacca ccttaaactt ggacaaccaa cgccaagctg 1800 gcctagcctt ctccgtccct ccatcgcaat aaccagaagt acaggaatat taacctgttt 1860 tccatcgact acgcttttca gcctcgcctt agggaccgac taaccctgcg tcgattaacg 1920 ttgcgcagga aaccttggtc tttcggcgtg ggtgtttttc acacccattg tcgttactca 1980 tgtcagcatt cgcacttctg atacctccag caagcttctc aactcacctt cacaggctta 2040 cagaacgctc ctctaccgca tcacttacgt gatacccgta gcttcggtgt atggtttgag 2100 ccccgttaca tcttccgcgc aggccgactc gactagtgag ctattacgct ttctttaaag 2160 ggtggctgct tctaagccaa cctcctagct gtctaagcct tcccacatcg tttcccactt 2220 aaccataact ttgggacctt agctgacggt ctgggttgtt tcccttttca cgacggacgt 2280 tagcacccgc cgtgtgtctc ccatgctcgg cacttgtagg tattcggagt ttgcatcggt 2340 ttggtaagtc gggatgaccc cctagccgaa acagtgctct accccctaca gtgatacatg 2400 aggcgctacc taaatagctt tcgaggagaa ccagctatct ccgagcttga ttagcctttc 2460 actccgatcc acaggtcatc cgctaacttt tcaacggtag tcggttcggt cctccagtta 2520 gtgttaccca accttcaacc tgcccatgga tagatcgccc ggtttcgggt ctattcccag 2580 cgactagacg ccctattaag actcgctttc gctacgcctc ccctattcgg ttaagctcgc 2640 cactgaaaat aagtcgctga cccattatac aaaaggtacg cagtcaccca acaaagtggg 2700 ctcccactgc ttgtacgcat acggtttcag gatctatttc actcccctct ccggggttct 2760 tttcgccttt ccctcacggt actagttcac tatcggtcag tcagtagtat ttagccttgg 2820 aggatggtcc ccccatattc agacaaagtt tctcgtgctc cgtcctactc gatttcatga 2880 ctaagagatt ttcgcgtaca gggctatcac ccactatggc cgcactttcc agagcgttcc 2940 gctaatctca aagccactta agggctagtc cccgttcgct cgccactact aagggaatct 3000 cggttgattt cttttcctca gggtacttag atgtttcagt tcccctggtt cgcctcttgc 3060 acctatgtat tcagtacaag ataaccatct tatgatggct gggttccccc attcagacat 3120 ctccggatca aagtctgttt gccgactccc cgaagctttt cgcaggctac cacgtctttc 3180 atcgcctctg actgccaagg catccaccgt atgcgcttct tcacttgacc atataacccc 3240 aagcaatctg gttatactgt gaagacgaca ttcgccgaaa attcgaattt ctcaattaag 3300 agaactcaca aattttacct tagcctgatc cgttaccagt gaaagtaacg ttcagtctat 3360 ctttctatca catacccaaa tttttaaaga acgatctaat caaagactag aaatcaacat 3420 tcaccatcac tttgatggaa tgctcatttc taagctttaa actttcagaa gcagtagtgg 3480 tggagccaaa cgggatcgaa ccgttgacct cctgcgtgca aggcaggcgc tctcccagct 3540 gagctatggc cccgtatttc tacaggcgtt tcccacacaa aattggtggg tctgggcaga 3600 ttcgaactgc cgacctcacc cttatcaggg gtgcgctcta accaactgag ctacagaccc 3660 aatttcgggc tgcttcttta tcgtcttctt caatgaatca agcaattcgt gtgggaactt 3720 atggagcagc tgatgtcgtc gattaaggag gtgatccagc cgcaggttcc cctacggcta 3780 ccttgttacg acttcacccc agtcatgaat cacaccgtgg taaccgtcct cccgaaggtt 3840 agactagcta cttctggtgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc 3900 cgggaacgta ttcaccgcga cattctgatt cgcgattact agcgattccg acttcacgca 3960 gtcgagttgc agactgcgat ccggactacg atcggtttta tgggattagc tccacctcgc 4020 ggcttggcaa ccctctgtac cgaccattgt agcacgtgtg tagcccaggc cgtaagggcc 4080 atgatgactt gacgtcatcc ccaccttcct ccggtttgtc accggcagtc tccttagagt 4140 gcccaccatg acgtgctggt aactaaggac aagggttgcg ctcgttacgg gacttaaccc 4200 aacatctcac gacacgagct gacgacagcc atgcagcacc tgtctcaatg ttcccgaagg 4260 caccaatcta tctctagaaa gttcattgga tgtcaaggcc tggtaaggtt cttcgcgttg 4320 cttcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt 4380 taaccttgcg gccgtactcc ccaggcggtc aacttaatgc gttagctgcg ccactaaaag 4440 ctcaaggctt ccaacggcta gttgacatcg tttacggcgt ggactaccag ggtatctaat 4500 cctgtttgct ccccacgctt tcgcacctca gtgtcagtat tagtccaggt ggtcgccttc 4560 gccactggtg ttccttccta tatctacgca tttcaccgct acacaggaaa ttccaccacc 4620 ctctaccata ctctagtcag tcagttttga atgcagttcc caggttgagc ccggggattt 4680 cacatccaac ttaacaaacc acctacgcgc gctttacgcc cagtaattcc gattaacgct 4740 tgcaccctct gtattaccgc ggctgctggc acagagttag ccggtgctta ttctgtcggt 4800 aacgtcaaaa caattacgta ttaggtaact gcccttcctc ccaacttaaa gtgctttaca 4860 atccgaagac cttcttcaca cacgcggcat ggctggatca ggctttcgcc cattgtccaa 4920 tattccccac tgctgcctcc cgtaggagtc tggaccgtgt ctcagttcca gtgtgactga 4980 tcatcctctc agaccagtta cggatcgtcg ccttggtgag ccattacccc accaactagc 5040 taatccgacc taggctcatc tgatagcgca aggcccgaag gtcccctgct ttctcccgta 5100 ggacgtatgc ggtattagcg tccgtttccg aacgttatcc cccactacca ggcagattcc 5160 taggcattac tcacccgtcc gccgctctca agagaagcaa gcttctctct accgctcgac 5220 ttgcatgtgt taggcctgcc gccagcgttc aatctgagcc atgatcaaac tcttcagttc 5280 aaacatcttt gggtttttaa gaaaccctaa acttggctca gcaatcgttg gttacatctt 5340 tgatttctcg cggagtaact tgtgatgctg ataatcttgt tgactatcag tctgactcca 5400 caagcaccca cacgaattgc ttgattcagt tgttaaagag cggttggtta agatctttcg 5460 tctcaaccga ggcgcgcatt ctacagcagc ctcatttgct gtcaagtgat tattttcaga 5520 agttttcgaa gatttcttca acaacttcaa ccacttgcgc ttccgatcac tcgttagcgg 5580 gaggcgaatt ctacagcgtt acacgctgct gtcaacacct ctttttcaac ttcttgcggg 5640 cttcgatgaa ctgaagcaac tgactgccga aacctacata actcattgtt taccaaggag 5700 ttttccgttt cgactgcgcc ggaagtgggg cgaattatag gcctccagaa tctgccgtca 5760 acct 5764 <210> 36 <211> 573 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_36 <400> 36 tggagtggtg aaacttgcgc cgtgctaaaa tacatcaaca aagcaaagag gattccacag 60 gatagtcaaa taccctgact ggctgccttt ggcgcaaaga gctagcaaaa accttacgtt 120 tcaaacccct ttcaggaagg ataccccagc cgttggggca ccaatatttc aaaaattaac 180 gactgacgtc gcagtaacat ggagcctgac gtgggttttt acgctggcgg aagaacgagc 240 gtttatccag gtggttgcgt agctcacgct acctgaataa gtgtaacaac tggttcacca 300 tgatgattga cctcggcggc agcggattac aggagcagac gctgcacttt accgattatc 360 ctgtgcaaac cagcattgat ggcggcgtgg tcacatggac tggcaatgtt atcgcttaag 420 aaactcaata acacgatgga tgagtttgat gatgttctgg ttgaactgga ttacagatgg 480 tttggatggc tggatgaagt cgttaaccgt gacctgctgg agtatcaata atgccatcat 540 tacgcgatta caaagcaaag cgccctaact ggg 573 <210> 37 <211> 402 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_37 <400> 37 ggcgccagtt tgaatattag acataattta tccttcaagt aaggggccga acccctgcaa 60 ttaaaattgt tgaccaccga caataccaaa gacgagcgcc tttacgcttg ccttttagta 120 cctcgcaacg gctgcggacg accagggcga gccgccagaa acgtttttta cctttagaca 180 ttacatcact ccttctgcac gtaatttttg gacgcacgtt ttcttctgcg tcagtaagaa 240 cgtcagtgtt tcctgcgcgt acacgcaagg taaacgcgaa caattcagcg gctttaaccg 300 gacgctcgac gccattaata atgttttccg taaattcagc gccttccatg atgagacagg 360 ccgtttgaat gttgacggga tgaacataat aagcaatgac gg 402 <210> 38 <211> 474 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_38 <400> 38 cgcagctcga gaagctctta ctttgcgacc tttcgccatc aactaacgat tctgtcaaaa 60 actgacgcgt tggatgagga gaagtggctt aatatgcttt ggcacgttcg tcaaggactg 120 ggtttagata tgagtcacat tttgttcatg gtagagattc tcttgttgac attttaaaag 180 agcgtggaat tactatctga gtccgatgct gttcaaccac taataggtaa gaaatcatga 240 gtcaagttac tgaacaatcc gtacgtttcc agaccgcctt tggcctctat taagctcatt 300 caggcttctg ccgttttgga tttaaccgaa gatgatttcg attttctgac gagtaaccaa 360 ggtttggatt gctactgacc gctctcgtgc tcgtcgctgc gttgaggctt gcgtttattg 420 gtacgcttga actttgtagg ataccctcgc tttcctgctc ctgttgagtt tatt 474 <210> 39 <211> 468 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_39 <400> 39 taaatgctgc gtcaccaaca ctaatccacg cgcctaaacc aacgccacca gttgatgcag 60 gagttgaacc agcagctaca gccttaggaa atgcaccatc ccatcggtaa tattctccgg 120 ttgcctcgta acgtagcacc tgatttggta gcgtccaccg tcgcgccatc ttccaaagct 180 atccattgtg atatatccat aagcacgaat ggcctcttcc gcagtgtact ggaatccagc 240 aatagtccag cgttttacgc caaatctgtc cacatagtaa tgtgcatcag atgtcatgac 300 ttcatcaaat ttaccagcgt taaacttcag gtcgcgcggg tcttctgacg ggattggtaa 360 gctagtcggt gtggtagcca ttataataaa tgctcctttg tgaatttgat gtatattgta 420 tcacgatgac gggatgacat attcgtacat gtcatcactg tattcgct 468 <210> 40 <211> 352 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_40 <400> 40 aaatgaagaa ttgaccatca tggtaacgct gcatgaagta atcacgttct tgtcagtatg 60 caaaattagc ataagcagct tgcagaccca taatgtcaat agatgtggta gaagtcgtca 120 tttggcgaga aagctcagtc tcaggaggaa gcggagcagt ccaaatgttt tgagatggca 180 gcaacggaaa ccataacggg catcatcttg attaagctca ttagggttta gcctcggtac 240 ggtcagggca tccacggcgc tttaaaatag ttgttataga tattcaaata accctgaaac 300 aaatgcttag ggattttatt ggtatcaggg ttaatcgtgc caagaaaagc gg 352 <210> 41 <211> 311 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_41 <400> 41 tggtggcgtt ggtttaggcg cgtggattag tgttggtgac gcagcattta gacaggaagc 60 caacaaaaaa ttcaaatatt cagtaaaatt atcagattat tctacttgca ggagcagcaa 120 cagcagcagt tgacggagtg ttgattgacc gcgactatac attcaccgac aacgagacag 180 ttgattttgg cggcaaagta ctaaccatcg actgtaaagc taaatttatc ggagatggta 240 atcttacatt tactaatctt ggcgcgggtt ctttagtaaa ctctccgtat atggaaagtg 300 ccacaactcc g 311 <210> 42 <211> 494 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_42 <400> 42 ccgccatcat ggtcaacccc gccatcttgg cgcaggaatt gaactgagct aacagaaccg 60 taagcaattc tcccacctat acagtagtta ccaaccccct tattaccggg ttagattcat 120 aaaggtaatt cccgcagccc cacctgctac cgccaaatca gaatcaacag ctttacaata 180 aaagcaagta tcaaacaggt agcaaccgac cgacccttta gcatttttta cagtaatacc 240 agtacagtta aaaatacgaa gtgtggaatt gaatatttgg tctttcgcat aactaggcag 300 caatgcctca atttcaagga aatttggtgt agtcgcttac ggttggttga taaaccccta 360 tccctgggtt tgtgccagcg ttgccgcaac tgccgcggcc tccgtaatcc aacttccatc 420 tccattccat ggaaaaatca tccacggagt tgtggcactt tccatatacg gagagtttac 480 taaagaaccc gcgc 494 <210> 43 <211> 551 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_43 <400> 43 tcatacatat gagcgtgcag gaatttacca tcaaggatgt ttgaatacag gcgaccgcga 60 ccgttttcag tgtcaaggaa tccgatttct tctggtgaat caaccatgcc tcgtgccagc 120 ttaagtgcgc tatatgtttt cccgctacca gactggcctg aaataccgat gaccacgcga 180 gaaccggaac gctctgctgg tttaatgttg agaatgccca tttgcatcac ctcactaatt 240 ataaattaaa ttgcttcttg aaccattcag gcgtttccat ttctataact ggattaccca 300 ttgaataacc cggccacgaa ttggcttttt tgcatgcttt gtaaattttc catagcattt 360 ctcaattgga tgcgacccaa tctgtaattg ctcatctgaa agtctaatcc ctgtatgtat 420 atacggagct gctttctctt gtaccagaag attaactgag cgcggagggt gaccatatgc 480 ctcaacaaac atgtcgtgct gcatcgccat cttcataaag taaccaagtc gcgcagcatg 540 ccggaaaaac t 551 <210> 44 <211> 782 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_44 <400> 44 cgcttgttcg ttttccgcct actgcgacta aagagattca gtaccttaac gctaaaggtg 60 ctttgactta taccgatatt gctggcgacc ctgttttgta tggcaacttg cccgccgcgt 120 gaaatttcta tgaaggatgt tttccgttct ggtgattcgt ctaagaagtt taagatttcc 180 tgaagggtca gtggtatcgt tatgcgcctt cgtatgtttc tcctgcttat caccttcttt 240 aaaggcttcc cattcattca ggaaccgccc tcttggtgat ttgcaagaac gcgtacttat 300 tcgccaacca tgattatgac cagtgtttcc agtccgttca gttgttgcag tggaatcgtc 360 aggttaaatt taatgtgacc gtttatcgca atctgccgac cactcgcgat tcaatcatga 420 cttcgtgata aaagattgag tgtgagggtt ataacgccga agcggtaaaa attttaattt 480 ttgccgctga ggggttgacc aagcgaagcg cggtaggttt tctgcttagg agtttaatca 540 tggtttcaga cttttatttc tcgccataat tcaaactttt tttctgataa gctggttctc 600 acttctgtta ctccagcttc ttcggcacct gttttacaga cacctaaagc tacatcgtca 660 acgttatatt ttgatagttt gacggttaat gctggtaatt ggtggttttc ttcattgcat 720 tcagatggat acatctgtca acgccgctaa tcaggttgtt tctgtttgtg ctgatattgc 780 tt 782 <210> 45 <211> 771 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_45 <400> 45 attcaggctc gcacgaatgg gaaggggaag gaagttgcac ggagattgct gaaaagcctc 60 tgcttaatgg caaaaagatg gctgactgga agcgcgccaa agctgaacac aagaagttca 120 tgaatgccat gctgcaaagc aatatgcata tcattgtatg tctacgcgct cgccagaaaa 180 ccgacttcgc aaacccggag ggaacctgta tcacttgggt tgcagccagt gtgcgagaaa 240 gacttcatgt tcgaaatgac tgtgagcatg atgatgcacg atggcggtaa aattcaggaa 300 ttcactaaat taccagaaga actacgccca atattctttg agtctggtcg ggaaagcgtt 360 cgacatggat acattggcga agcgcacggt cgcggactca tcaagtgggt ggactctggc 420 gttaaagttg acgaagagtt tgaatcttgg cgttcacgac ctccagcttt ccgccgcaaa 480 aggcatggaa ggattgaagg aagaagcaaa atcaataact tgataacctg aaagataaaa 540 ttcgcgcaat ctggccttct ctcgctgctt ctgctgcaga atacgatcgc attgagtctt 600 tcattaatga tgagcaatta ccacaggtgg taatcactcc tcaggataat ttcgaaccct 660 gcaaaactgg ctaaaccgaa accacaacag ccagaaccgc aggaagaaac aaaaaccgaa 720 cataaaccaa ctccaattga aggattttaa taaatggcac gcggcgtaaa c 771 <210> 46 <211> 696 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_46 <400> 46 ttgctggcaa gcgcaagaat ggcggccctg ttagcgcagg aagcatgtat gaagtgggcg 60 agggaaatgc accagaaata ttccaggcga gcacaggcag gcaatacatg atccctggca 120 actcaggaaa ggttatcagc aataaggata tttccggcgg aggtggcggt gttgtggttt 180 ataatagcgt tatcaacaac agctcagcgc aggttagcag ccagcgccag agataatggg 240 gacggcagcg tgacaattga gacgattgtg agtgattatt tcggaaaatg gcccaatagg 300 gcaagccatc tcaagaaact acaacaccaa cagacgagca acagagtaaa aagaagcccc 360 ttatggggct ttgttttaaa tcttatcagc gcatttacgt acgaccctcg gtatcgtttc 420 tttcagttca tcccatgagt tgaccgcttc catatccaga agtgtgacaa gagacttctg 480 gatatcactg cgtaactggt tagtttttgc atcctgagag tcccatgtgt caaacccagt 540 aacttcacgg atatctatcg actccttgac tgactttgcc acctgaatga aatgcccact 600 atgaccaatt ctaccctcag tgcgttcaag cattctgctg acggcagcat taagttccac 660 gaaaacttac cgcaccaata tttcgcaact caaccc 696 <210> 47 <211> 299 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_47 <400> 47 atgcggtcaa tttccattcc atccggaata gtaccttaat gcatttccca gatcaccctg 60 tgcgcagggt gttttttgcc tttgaaatca aataaaagat agcctttatt gttttttgac 120 cagcaatgtc accaacctta accctattcg caggccttat cttccaatac aactgaccat 180 cacggtattc aaatacatca ttccaattca tcatcacccc ttttcattgt gactttgtta 240 ttctcgtcaa cattgaagtt gtccctgata aggttataca tctcatcttt aggcatatc 299 <210> 48 <211> 403 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_48 <400> 48 agcacagcgt caaacagcat taccggatta ctgacaggca caatgagcgt tcaggatgcc 60 atgcgcagcc tcggctctac ggtgctgaat tctctggtaa acagctttgt tgaaatgggc 120 gtgcagtggg tgaaatctgc ggttatgggg cagacagctc aggtagcagc aaccgctacc 180 acaacggcgg cgcaaacggc aggactggca accaccacag ctgcctccaa ctgcggctgc 240 agccaccacg acggcggcat ggacccccgc tgcaatcgtt gcgtcaatcg gttcattcgg 300 cggcgcagct gcaattggtg taggcgctgt ccttggggca ctggcgatgg gtattgctgg 360 caagcgcaag aatggcggcc ctgttagcgc aggaagcatg tat 403 <210> 49 <211> 1115 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_49 <400> 49 tcggcggttg cagaagctga gtgcaacacg gttattgatt tgaagctgga gcatgatgcc 60 gcatagccat ggcttcctgc atcacctcgc ccatgacttc gatcgggcac ttgaaatcga 120 agcgtttacg ggggcgcata ttcagctcca aggcgatggc attcaattgc tcttgactgt 180 gtaccgacaa gtcagtcccc ttgggcaggt actggcggat caggccgttg atgttttcgt 240 tgctaccgcg ctgccagggg ctgtgcgggt cgcagaagta aatagcgacc cctgtctgct 300 gggtgatttc agcatgccgc gccatctccc ggccctggtc gtaggtcatg ctcttgcgca 360 tcgccagcgg catgctgttg agcgctgcac tgaagccctc catcgccgag gtcgccgttg 420 cgtcgttcat cttcatcagc atcaggtagc cactggtgcg ctccaccaac gtgcctaccg 480 acgaggcgtt ggccttaccc ttgatcaggt cgccctccca atgccccggc atcagccggt 540 cttcgatctc tggcgggcga acatgaatgc tgaccatctc agggatctgg ccgcgccgat 600 ccacgccacc agagcgcggc cggcgcgtcg tcttgccttg gcgcaggcag acgatcagct 660 ccttgcgcaa ctcgccgacc ggcaaggcat agatcgcgtt atagatcgtc tcacgacaga 720 cgtaggcatc tctgaggttg ggtatggtca tgctgcgcag cttgccggca atctgctcgg 780 gagacaaacg cttacgcagc atgtgggcca ccagttcgaa gcgctcgctg cccggcacaa 840 gcttgcgctt ggggcgacaa acctggcgcc gaatgttcat ctgttgctgg gcgcagcggg 900 ccgagtaagc accactactg cctcgattgc gcttcagctc tcggctgatg gtcgagggcg 960 acctgtcaat caggcgagca atgccgcgca ggctgaagcc ttgagcttga ctgagttgaa 1020 tgatggcgcg ctcttcgacg ctgagttcgg aataggacat aggggcagca ccgtaccgga 1080 aaggtcaggt gttgcactca gttttcgcgg ccgcc 1115 <210> 50 <211> 1197 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_50 <400> 50 ttagggatgg tctgaagtag ccatgtattt ccccggcata ccacctagcg caacttttaa 60 actcgccaaa tgatcaaaaa acaatcaatt cgacgagtat ttcttacgtt ttcgccaccc 120 ggaccccact ccggtggcca tttcccgcat tacaggcgca cctctcctgc attcgtcagt 180 aaatgtcggc gcgccatcca caggttcgac agagcgaaca gtgtcaccag ttgcgccgtg 240 tttttggcca agccgcgaaa gcgcaccttg gtgtaaccga actggcgctt gattacccgg 300 aacggatgct cgaccttggc gcgcacctga gccttggcct tttcaatctt gcgcttggct 360 ttgtacagca cgctgcgctt atcgagtttt ttgtaggtgc tgcggcgtgc cgccacctgc 420 cagataactt cacgtccttc atgctcgggc cgcttttcga caccggtgta gcctgcatcg 480 gcgcagacca cgttttcgtc gccatgcagc aatttgtcca cctgggtgac atccgccaca 540 ttggccgccg tgcccactac gctgtgcacc agacccgatt cgtcatcggc gccgatgtgg 600 gccttcatgc caaaataata ctggtttccc ttcttggtct ggtgcatttc cgggtcgcgc 660 ttgccgtcct tgttcttggt cgaactgggc gcattgatca gcgttgcatc gacgatggtg 720 ccctggcgca gcgacaggcc gcggtcgccc agatagccat tgatgacagc gaggatgccg 780 gccgccagct cgtgtttctc cagcaagcgg cggaagttga gaatggtggt ttcgtcgggg 840 atgcgctcca ggttcagccc ggcaaactgg cgcaggatcg tggtttcgta cagcgcttcc 900 tccatcgctg gatcgctgta gccgaaccag ttctgcagca gatgcacacg cagcatcgcc 960 atcaacgggt aggccggacg gccaccttca cctttcggat aatgtggctc gatcaaagca 1020 atcaagccct tccacggcac cacccgatcc atctcgatca ggaacaactc cttgcgggtt 1080 tgcttgcgct tgccagcgta ctcggcgtcg gcgaaggtca tctgcttcat cgggaaactc 1140 ggtgggtggg gtcgcggtat tttgccaaat cagaaagtct ttttcagagt ttcccta 1197 <210> 51 <211> 650 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_51 <400> 51 cggcatcact ttaagatgct ggatcagcag gcattgaacg aaaagctcca ctcgacccag 60 agaattttgt ccgggttgaa cagcatcgat caattcagcg aatttaagcc tgagctggag 120 gcgctgctgg gggctcaccg tgatttgact gccctaataa tcgatagtga cggcaagctg 180 ctgtttgctg atcccggtcc ggtagatatt ccgaaggagt tccgcacaac cacaaacaac 240 aacgtctggg agtggcggga gcaagggcag atgttccgtg gagtgacggc acaagccgtt 300 gtgacggggc aaaacaagcc gttgacagtc attttgatct ttgacgttac tcagcatatg 360 tcctttttcg agacgcttga acgatggttt tggatagggt tggtgatcag cgcattggtc 420 agtgctgcac tcggctggat ggtagcaagc agtggcatgc ggcccattcg ccaggtcacg 480 cgggtcgctg cctcaatgtc agcaaaatca ctcaaagagc gcatcccctt agcacccgtt 540 cccaaagagc ttcagcaaat ggtgctgtcg tttaacgcaa tgttgtccag gctcgacgat 600 gcatttgtcc ggttatcgaa cttttccgca gacattgccc acgagctacg 650 <210> 52 <211> 2580 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_52 <400> 52 tgttggcgct gattgaaaac tgacccaccc tgccgattga aaattgaccc agggcggatt 60 gctgattttt gtcccagcaa ttgtggataa gcttagcagt agtgtcctag ttgaagacgc 120 atctggcccc accgcatgca ggcatgcgga agggtgttta cggtgcagat caaaacggct 180 catcgtcctc aataccatct tcgtccttgc gctttcgttc acgcgatttg atctttgtct 240 gagcggccaa ggtgctgtgt tgcaggcggt aggactcgtt gcccgtttcg acgatatggc 300 agtggtgtgt cagccgatcc agcaacgctg tggtcatctt ggcgtcgcca aacacactcg 360 accattccga gaagctgaga ttggtggtga tgaccacgct ggtgtgctcg tacagttttg 420 aaagcaggtg aaataacagc gcgccaccac tctgactgaa gggcaaatac ccaagctcat 480 cgagtatcac caggtctgtc cgaagcagtc cctgggcgat tcggcctgcc ttaccatcgt 540 acttttcacg ctccagcagg ttgacgagat cgaccgtgga gaagaaccgt actcgtttgt 600 tatgagtggt gattccggac acggccaagg cactggccag gtgtgttttt ccggttccag 660 gcccaccgat gaacacaaca ttttgcgcag tctccgtaaa ctcaaggctg gataattcct 720 tgattagacg ggcatcagcg ctggaagcac tgaagtcaaa gccagctaaa tcacggtgca 780 tggggagctt tgccatgttc atctgatgat tcactgagcg cacggcgcga tctgcctgct 840 cctgttggag cagatgctca agcagccact tcgaggatgc cgtcgaagct tctccctggg 900 ccgccagttc ttcccaagct tgcgccatgc catgcagacg cagttcttta agttctgcca 960 tcaagtcacg cattgcggct ctcctcatcg gttgtacgga gttggtcata tcgggccgtg 1020 ttggcaacgg gagccacctt gagttgcagg ctggtttcta cggaaggcgg tggtgcagta 1080 gcggtgagtc gggcgagaac atttaagatg tgatcggcgc tcagacttcc cgactcaagc 1140 accagttcta ccgctaccag caccgggtcg agaccggcga tgggcacggc agcgagcacc 1200 tgcgtcatga ttcgatcacc attgctgtga cgtctcaggc cccgtttgag aagccgcaac 1260 ggattgggca gatcggcaaa cggtgcgcca ttgcgcagtg cacccggctt gcgttcgatg 1320 agtgggatgt aatgctgcca gtcaaaactg acctgatctc gatcaaagag gcgctcgtgg 1380 ctggcaatca ttgcgtcatc ggcgatgacc acgatccggg atgggtaaag acggctgctg 1440 acccactggc cgactcgctc acaaggcacc gaatagcgat ttcgcgccac gctgatcagg 1500 caggtgctgg agacccgaac ggtgcgctcg acgtagccat caaaggccgt cggcatcggc 1560 atcatttcga cccgttccag ctccaaaact tccgccaccg taagtccgtt gtactgcgga 1620 tgcagcagct cgctccagag cgcacgacag cgttggccga gccaggcatt cagttcctcg 1680 aaggtgtgga acatgcagtt ttgagcgtca agccaaatgc gccgtcgact gtcttgaacg 1740 ttcttctcga caatgccctt ttcccagcca gaggctacgt tgcagaaatc cggatcgaac 1800 aggtagtgcg cgcacatcac ggaaaaccgg gcattgaccg tgcggccctt gcctttattg 1860 accttgtcga cagccgtttt catgttgtcg tagatgccgc gacgcggcac gccacctaac 1920 gctccgaacg agcgcgtatg tgcatcgaat agcatctcgt gaccttggct agggtatgca 1980 accaaccaga atgcgcgact ggcgcacagc ttcatgtggg agacctggat gcgtcgaaac 2040 aggcctccga tcagcagccc ttcttcgctc cagtcaaatt gaaaagcttc accgagcgca 2100 aacgtcagcg gtacaaaagc gcgtgaagac ttgccttgtt cacctcgcca cgagcgtacg 2160 aacgccgtga gctggctgta gccgccgtca taaccctcgg ccttgatctg ctcgaaaagt 2220 gctttggcgc ttctgcgatt gtgctttgcc cggaacgaat cggctttcag cgcctgttcc 2280 agcgtgtcgt ggaaagggct gagtttgttg aaggtcgcgc accgctggta cgccggctga 2340 gtagcttcgg gcgctctgac ccattttcgg atggtgtttc tcgacagccc ggtacgcttg 2400 gctatctggt gcagcgagag cttgtcgcgg aagtacatcc gccggatttt tcccaacatt 2460 tccatgctga tcaccctgtg ttctcctgct cggaaagtga gcagaagcag ttgaacacct 2520 gggtcagttt tcagtcggca gaacagcctt tactgggtca gttttcggtc agcggcaaca 2580 2580 <210> 53 <211> 2297 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_53 <400> 53 gtaaccgtcc ggccagcacc ctctcctgac agccctccaa attagccaag atagtggaca 60 ccatttttta gtggacacta tcttggatac agttgccccc gctcgtcgaa ccggtcgccg 120 ccccaacttt tcgctgacgt tcaaacgcca agtcgtcgag gccaccctcc agcctggtgc 180 ttccgtgtcg ttgatcgctc gtgagcatga cgtcaatgcc aacttggtct ttcgctggcg 240 gcatcaatat catgaaggga tgttcggccc gactaaccag agcgcaacgc ttttgcctgt 300 tcaggtgatt gaggcgtcga cggatgcatc gcaaaccctc cagccacagc ccgtatctta 360 ttccgagatc gtgattgagc ttggaaaagc caagctgcgc atcactggca cgcctgaccc 420 gcataccctg gaagtcatct tgcagcagtt actgcgatga ttgccccgcc tgctggaacc 480 cgtatctgga tcgccgccgg cgtcacggat atgcgccgtg gcttcgatgg attggcagcc 540 ttggtgcaaa ctcaacttga agccgatcca ttctccggcc agatttttgc ctttcgcgga 600 cggcgcggtg accggatcaa gctgttgtgg tgggatggcg acggtttgtg cctgttttgc 660 aaacggctgg aacaagggcg cttcgtttgg ccgcaagcta ccagcggcag cgtgtcgcta 720 acgcctgcgc agttgtcgat gctattggaa ggcattgatt ggcgcaggcc cactcgtaca 780 gcgcccgtcc ttgcggtctg acttgccacg ctgaaaaaat cccaagtaac attcggtcat 840 gactatcgcg accgactcct tgcctaacga tcttcaagcc ttgaaagctc tggtctctgc 900 ccaacgggca gagattgagc gcttgaaaat gatgatcgcc aagctgcgtc gtatgcagtt 960 cggtcgcagc tccgagcaac tcgacgcgat gatcgatcag ctccaactga gtcttgaaga 1020 gttgcaagtc agccagactg aactgacgcc gccaaccgaa ccgccacctc gccctgtctc 1080 tcggcgcaag ccattacctg aacacctgcc tcgcgaaatt cacgtccatc agcccgaatc 1140 acagtgttct ggttgtggtg ggaaacttcg ccacttgggg gaggatgtgt ctgaggtgct 1200 ggagtacgtc ccagcgcgtt tcaaagtgat ccgccatgtt cgccccaaat gggtttgccg 1260 ctgctgtgag cacattgctc aggtaccggc gccgagtcgc ccgatcgctc gaggctttgc 1320 aggcccgggg ctactggccc atgtgctggt ctccaagttt gttgaccatt tgccgttgta 1380 tcgacaatcc gagatctacg cccgtgaggg cgtggatctg gagcgttcaa ccctggccga 1440 ctgggtcggc cagagcagtc agttgctcag gccactgatc aacgcccttg agcaccacgt 1500 catgagcggt cacaaagtcc atgccgacga cacgccaatt ggcgtgctcg cgccgggcaa 1560 cggcaaaacc aaaacagctc gcctgtggac gtacgtgcgc gacgaccgac ctgctggtta 1620 cacgacaccg gcggcggtct ggtttgccta ctcgccggat cgcaagggag aacatccccg 1680 cgctcatctc aagagtttca gcgggatctt gcaggctgac ggctatgccg gttttgctca 1740 gttgtatgcg acgggcatta ttcaggaagc cgcctgctgg gctcatgccc ggcgcaagtt 1800 ttacgatgtc tacaaagacc aggcttcacc acttgctggc gaggcgctgc aaagaattgc 1860 tgccctgtat gccatcgaga gcgaaattcg agggcaaccg cctgaccaac gcaaagcgat 1920 tcggcaaagc cgggccagtc cgctgctgga acaattacac gcatggctta accagacgct 1980 gactcaactg tcgaaaaaat cggcattggg cggtgcgatc ttctatgccc tcaatcggtg 2040 gcaggcttta atgcgctact gcgatgacgg ccgaatcgaa atcgacaaca acaccgccga 2100 acgggcgctg cgcgccgttg cgctgggccg caagaactat ctgtttgccg gttccgatgc 2160 cggcggagaa cgcgcggctg cgatttacag cttggtgggc actgccaaat taaacgggtt 2220 aaatccgcag gcgtatctga cctacgtgct ggagcatatt gccgagcacc caatcaatcg 2280 ggtaagcgag ttgctgc 2297 <210> 54 <211> 417 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_54 <400> 54 agtgtctttt cctgcggcag tgagcactgc agagccgccc ttgattttcg taaggccgaa 60 tcaatcaaac acgttctgct tttgcaaagt tctgggctct cccggcgcct ggacgggttt 120 ttcgttcgtt ggtgtttcag ggcaaggcac atcgggatcg tggttttgcg ccgcatttaa 180 gtcgaatgct acggctaagt ttggacttag gttgtgggat gcttcaagta aattgctttt 240 cgataatgga actcaagcag cacagtttac gaggactatt gcgggctggg atttcattgg 300 gtccgagcaa acaagtcagg gcgtgtatcg gctaagttgg actgcctatt cgccgcttaa 360 cacaggcgac tatatgttgc tgaacaacat cgcgatggat gttgctggga gtacttc 417 <210> 55 <211> 245 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_55 <400> 55 tatccatttt ggccttatct gattctgttg atctgtccat tgatgcattt ttttggacat 60 ggacatggcg gacatagcca tggcgatcag gcagcaaccc acaaggacga aaaaggggaa 120 tagccatgcc taccatatcg agccaccacg accacagtca cgcccatgct gctccgctca 180 atgacgatga tctacgtgac ccggtgtgcg gaatggcagt cacttcatca agtaagtttg 240 gtgag 245 <210> 56 <211> 557 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_56 <400> 56 tgagccgtgc ccagcaccaa tggccaaccc cacacgacca tgaaccacag ggcgagcacc 60 tgcgacagtg tcgacttgcc gttctgcctt gcgacgagaa cgaccaccgt gcgaaaacgc 120 aaggtgttgt cggggagaag ttcgagcatg tggatcagca gccaccgctg ccacgggaac 180 aggtcaatcc cgatcgccgt agcgaagtcg atgacgtcga aaccgagcgt cgtctcccgc 240 gtcaactccc gaagtggcgg ggtgaacacc cgcggcacct cggaaccgag acgcttagac 300 cgctcggcgg cgcttggctg cctgggcttg gaggtcgccg agcttgttgc caccgctcgc 360 ctccggcttc tggtccccga gacgcacacg gccggcagga gtcagcccca acgactcggc 420 gtagcgcaga taggtaggga tcgtcacgtt gtcgagcttc cccgcagggt tcaagcctga 480 gtcgtcgatc aggtcagccg ccttcgaaag ggaaagcagc atggcgaccg cgcccgcgtc 540 ctcgtccgtc aggtgct 557 <210> 57 <211> 638 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_57 <400> 57 tctcgcgccg tgaggaagag cacgggaaca tcttttccgg atgcacgaac cttctgaagt 60 acttcccagc catccagccc aggcatcatg acgtctagaa tcagcaggtc ataggcctcg 120 ctcaaagcgt gctgtagcgc caccttgcca ttggtaaagc gatccacatt gaaccccgcc 180 tccgtgagtc cttgctgcaa atagatacct attttgggtt cgtcttcagc aaccagaagt 240 ctcatgaggg tggctctcgg ataatgatga agcgcagtct gcaacgaaac acgccagcca 300 accagcaact gacagaaaag taatattggc ttcaggtaga tgtcagcgag cgctgtctag 360 ggttcaagca tgagtacttc ctggtgctcg accgactctt cagcatgaaa cgttagggag 420 ccggaaaaca attccataag gagttgcccc atgaatcgcc tgaaaatgct gttttttgtg 480 agctcgatac tcgtctcagc ttctgtatgg gctgaaagtg gcggagaccg ggtgatagag 540 cgtatggaag gcctgcgcga cagggctgaa gccgtactga tccaagctga aaaagcccct 600 gaaggacagc gtcatgtcca catggccgag catatgaa 638 <210> 58 <211> 544 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_58 <400> 58 cctcgaatca acagctcttg ctgtgaggag tcttgcatgt cattccttaa aactacgacc 60 gtagctgttg cacttaccgc tggtttgctt ctgagtgcag ttgctcaagc acatccaaag 120 ctcctttctt ctactccggc agaaggctcg aatgcggcgg ccccttcgaa aattgaactg 180 cacttttctg agaatctcac cactcagttt tccggtgcaa aactgatcat gaccgatatg 240 cctggcatgc cgagctcccc aatgggtgtt aaggccgccg tcgctggtgg cggtgatccg 300 aaaacgatgg tgattacacc ggcagcacct ttgaccaccg gtacctacaa agtcgaatgg 360 cgtgctgtct cctcggacac tcacccgatc accggcaact tctcatttaa agtgaaatga 420 tttatgagcg actcaataaa tattgtggtt cgctttgctc tttatttcga cctgatgctg 480 ttattcggat tggcaatttt cggcttgtac agcctcaggg gaaaggaaag agtatcgggc 540 acgg 544 <210> 59 <211> 372 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_60 <400> 59 tcatccaggc cgtgatgggc tggagtgata gccatctgca tgagtttgaa attgccggtg 60 agcgttatgg tatgcccgat cccgatggct gggggccggc ggttaacccg gaagctcgta 120 aaacgctgat caaggcgctg aacggaaagc ggacatttaa ttatctctac gactttggtg 180 acagctggca tcacagtatc aaggtcgaaa aaacgttacc cgctattgcc tgtcctcagg 240 cgccgtattg catcgatggc gccaatgctt gcccaccgga agacgtgggt ggcgggcctg 300 gctatgaggg ctttttggca gcgatggcca accccgatca tccagagcat gacgacatgg 360 ttgaatggca tg 372 <210> 60 <211> 253 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_60 <400> 60 ttttgcgtta tgaactcgga catatcgtcg ccgttgatta ccagattcag cgggcaaagg 60 gttatgagcc ctacgtctgg gtcactgagc aaagtgtttt ttcctacgcc tacgacgaac 120 ttggacggtt ggtttcggcg accaatgccg tcggcgaaag tgagatttat cggtacgacg 180 atcagcacgt cattcttgaa cgtggtctgg ccggtggagc gagtttcttc tgggagtggg 240 aacggtctgg caa 253 <210> 61 <211> 309 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_61 <400> 61 atcaccaccg cactcacacc cagccaggtc gaggtgtggc cagcgtcccc gccatgagcc 60 tgccatgact gtgcatatgc gagacgccac ccacgattcg atagattttc ccatcctttc 120 cgcaggtcac tcggtcgcct tcaaaggcat gaaaaagggc atgggttttg attccgttat 180 caccatccag aacttcccct ccgcaggtgg ttttgtcacc caggcctatg taatagcctt 240 cagtcattat tcactcctcg atatgcctcg aacgagttgg gcatgtttaa aaccatcact 300 gaacctacg 309 <210> 62 <211> 310 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_62 <400> 62 ttgtagtagt ccgattgctt cttgagcttg gccatgacgc gactaggatc ttcatcggtc 60 cagtcagtca acatcaccac ataatcgcgg ttgtattgaa aaggttcagg ctctttcgag 120 tcgatgacga ttggaccgta aacgcctgcc tgctcctgca aaccagagtg gctgtggtac 180 cagtacgtac cgttctgatg caccttgaat ttgtactcgt acatgccatc gggcgcgatg 240 ccatggaaac tcaaacccgg tacaccgtcc atattggcgg gcagaatgat cccgtgccaa 300 tgaatggagg 310 <210> 63 <211> 268 <212> DNA <213> Unknown <220> <223> Salmonella Typhimurium KFS-ST contig_63 <400> 63 atttggtctc tgttggaaat gtcaaaaatc gccgggatga tcatgatgcc aaagtcacac 60 atccgcttag gcgacttgga ccccgtagac tttgaaaact tcccgcctca tctgaaacgt 120 cttctcacaa tcgttacgac gatgatcttt acctccgtaa tttggatggg agctgcagcc 180 attctagtga agctaaaata aatcaagggg gactaattta ataccgctta cagataactt 240 tctaaccaca attgaacctg cgttgaat 268

Claims

살모넬라 타이피뮤리움(Salmonella Typhimurium)에 특이적으로 사멸능을 가지는 박테리오파지 KFS-ST(KCTC 13465BP).
제1항의 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 살모넬라 타이피뮤리움균의 감염 예방용 조성물.
제1항의 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 항생제.
제1항의 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 소독제.
제1항의 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 사료첨가제.
제1항의 박테리오파지 KFS-ST(KCTC 13465BP)를 유효성분으로 포함하는 세척제.