KR102073760B1 - Cosmetics having anti-oxidant and anti-wrinkle and Manufacturing method thereof - Google Patents

Abstract

The present invention relates to an anti-aging cosmetic product and a method for manufacturing the same. More specifically, the present invention relates to, as a cosmetic product having optimal compositions and composition ratio using an extract of Pueraria montana, a multi-targeted anti-aging cosmetic product having excellent skin protection and skin regeneration effects by increasing the activity of Nrf2 in the skin to suppress skin damage caused by active oxygen (ROS), and having an excellent skin elasticity effect through the enhancement of collagen and hyaluronic acid synthesis.

Description

Antioxidant and anti-wrinkle cosmetics and its manufacturing method {Cosmetics having anti-oxidant and anti-wrinkle and Manufacturing method

The present invention relates to an anti-aging cosmetics and a method for producing the same using Root Root Extract.

Skin aging can be largely divided into two types. As shown in the table below, aging is classified into physiological aging, intrinsic aging, and photoaging due to external factors.

Photoaging is an aging phenomenon observed on the skin of the face, back of the hand, back of the neck, etc., which has been exposed to sunlight for a long time, and it is well known that ultraviolet rays are the main factor. Aging caused by UV accumulates in proportion to time, and continuous UV irradiation causes changes in both the epidermis and the dermis. In addition, the mechanism by which ultraviolet rays cause acute and chronic skin reactions is not yet clear, but reactive oxygen species have been reported to play an important role. In other words, in the skin exposed to ultraviolet rays, a large amount of free radicals are produced, which causes the transformation and loss of function of nucleic acids and proteins, the lipid oxidation of the cell membrane and the damage of the membrane function, resulting in decreased cell function and death. It is known to cause changes, especially in the dermis, which increases the activity of matrix metalloproteinases (MMPs) by ultraviolet rays and decreases the synthesis of collagen and elastic fibers (elastin), which causes wrinkles. have.

There is a wide range of metabolic systems in the human body that remove free radicals. Bioscientists have consistently researched and identified a broad spectrum of free radical scavenging metabolism systems that remove free radicals in the human body, and they are named phase I, phase II and phase III metabolic systems, respectively. All of the proteins involved in the Phase II reaction have an ARE sequence in the promoter, so Nrf2 increases the expression of phase II proteins at the transcriptional level that removes free radicals through ARE binding, thereby activating the human body. It has been found to protect from oxygen. Recently, many studies related to photoaging have increased the report on the molecular and biochemical changes occurring in dermal fibroblasts, and the role of Nrf2, a phase II detoxification enzyme, prevents apoptosis of dermal fibroblasts by UV rays. It has been reported that inhibition of Nrf2 activity can induce aging early (JID, 2005; 124: 825-832; MBC, 2013; 24: 1852-1862).

Most antioxidant cosmetics on the market today inhibit skin contact from factors that promote the generation of exogenous free radicals, or add antioxidants that can act directly on free radicals to inhibit skin aging. Is adopted. However, substances that inhibit skin contact with external factors reduce the skin's moisturizing ability or are irritating, and most of the added antioxidants in cosmetics have very low stability. Often difficult to obtain. For this reason, instead of cosmetics that passively protect the skin from free radicals, the manufacture of functional cosmetics that remove free radicals in a more aggressive manner has recently begun to attract attention.

Therefore, functional cosmetics using various natural plants have been developed and sold. Among these natural plants has been used habitually roots for food, and flowers, stems, etc. have been used as a medicinal herb. In the case of patents used in cosmetics, in the case of 대 the predominantly whitening effect is due to the antioxidant activity and tyrosinase inhibition. US Patent No. 5234706 has been filed for food using starch.

United States Patent No. 5234706 (document date 1993.08.10) Korea Registration No. 10-0628650 (Notice date 2006.09.26)

Taken together, photoaging is a phenomenon in which free radicals generated by UV light cause a combination of extracellular matrix synthesis and various biological mutations. The most effective response mechanism may be an antioxidant mechanism through Nrf2 pathway, a defense mechanism in skin tissue. have. In addition, if the proper concentration and activity of estrogen in the skin tissue is maintained, representative aging factors such as skin thickness reduction and wrinkle increase due to photoaging may be improved. That is, the inventors of the present invention will find that the multi-targeted cosmetics that can control the antioxidant mechanism and the synthesis of collagen and elastin at the same time will be effective. Knowing the aging cosmetic ingredients has completed the present invention. That is, an object of the present invention is to provide an anti-aging cosmetics and a method for producing the same using the Root Root Extract.

An object of the present invention for solving the above problems is to provide a method for producing anti-aging cosmetics by mixing with Root Root Extract and cosmetics.

Another object of the present invention is to provide an anti-aging cosmetics prepared by the above method.

The cosmetic of the present invention increases the activity of Nrf2 in the skin, thereby protecting the skin by inhibiting free radicals (ROS) and skin rejuvenation effect by inhibiting skin damage caused by active oxygen (ROS), multi-target excellent in skin elasticity effect through the synthesis of collagen and hyaluronic acid It is possible to provide anti-aging cosmetics and to provide various types of cosmetics such as various formulation toners, emulsions, essences and / or cream types.

Figure 1 is a schematic diagram of the root extract extract prepared in Example 1.
2 is a result of Western blotting (Western Blotting) measurement performed in Experimental Example 1.
Figure 3 is a result of measuring the relative expression amount by standardizing the protein expression experiment in Experiment 1 by the expression amount of the actin (actin), the reference protein using the Image J program.
Figure 4 is a cosmetic sample photo of the various formulations prepared in Preparation Example 1-4.

Hereinafter, the present invention will be described in detail.

Root is a perennial root plant with about 10 species distributed all over the world and 1 species (Peraria Radix) is growing in Korea. As a vine shrub, it grows wild in mountain fields all over Korea, and it is about 10m long and its roots are long, so it stores a lot of starch. , Make a rib. In oriental medicine, it is known to have the effects of sweating, fever, jingyeong, haegi, soojin, jigal, and branch office. The flowers, leaves, roots, etc. are known to be effective.

Roots are very safe for civilian use, and in some parts of Africa, they are grown as a food source because of their high starch content.

The anti-aging cosmetics of the present invention is a cosmetic using ingredients beneficial to the skin of the skin, mixed with the root extract and cosmetics, various formulations, for example, various formulations, such as cream formulation, toner formulation, emulsion formulation, essence formulation It can be manufactured as a cosmetic.

First, the method for producing the Root Root Extract in the cosmetic composition of the present invention, the Root Root Extract is a butylene glycol extract, after washing, the dried Root Root is mixed with butylene glycol, and then left To obtain an extract followed by primary filtration to separate the primary extract from the residue; Performing a second filtration of the primary extract; It may be prepared by carrying out a process comprising a; three steps of tertiary filtration of the mixed solution mixed with the secondary filtration extract and preservative.

In the butylene glycol, it is preferable to use butylene glycol at a concentration of 95.0 to 99.99% by volume, and preferably butylene glycol at a concentration of 99.0 to 99.99% by volume. At this time, the remaining amount of 100% by volume of the butylene glycol may be water.

In addition, in the first step, the mixed amount of the roots and butylene glycol is 5 to 15 parts by weight of the roots of roots, based on 100 parts by weight of butylene glycol, preferably about 100 parts by weight of butylene glycol, roots of 8 After mixing 12 parts by weight, an extraction process may be performed. At this time, when mixed with less than 5 parts by weight of root, there may be a problem of too little components such as isoflavones in the root extract, and when mixing more than 15 parts by weight, there may be a problem that the odor rises in the extract , It is good to perform the extraction process with the mixed solution mixed in the above range.

Then, the mixed solution obtained by mixing the primary extract of the first and second stages with the butylene glycol for 1 to 4 hours, preferably for 1.5 to 2.5 hours under the temperature of 180 to 220 ℃. It can be left to obtain a first extract. Further, the second extract may be obtained by leaving the mixed solution mixed with the residue and butylene glycol under the above conditions.

At this time, if the temperature is less than 180 ℃ extraction is not good, the content of the active ingredient in the extract is low, there may be a problem that the extraction time increases sharply, if the temperature exceeds 220 ℃ the problem and extracts that have a bad odor in the extract There may be a problem of too high viscosity. If the extraction time is less than 1 hour, the extraction time may not be sufficient, and there may be a problem of low content of the active ingredient in the extract. If the extraction time exceeds 4 hours, the extract may smell, and the viscosity may be too high.

Filtration of the first to third steps is for removing foreign matter in the extract, it can be carried out by a general filtration method used in the art. In addition, the extract of step 1 has a very high viscosity, so even if the residue is separated by primary filtration, foreign matter may be present in the extract. Therefore, it is preferable to additionally perform secondary and tertiary filtration.

In addition, the three-stage preservative may be used to minimize the skin irritation, preferably one or more selected from phenoxyethanol and 1,2-hexanediol. In addition, the amount used is preferably 0.1 to 1.5 parts by weight, preferably 0.2 to 1.3 parts by weight, and more preferably 0.2 to 1.2 parts by weight based on 100 parts by weight of the secondary filtration extract.

The anti-aging cosmetics of the present invention may include 2 to 15 parts by weight of Root Root Extract, preferably 4 to 10 parts by weight of Root Root Extract, based on 100 parts by weight of cosmetics. At this time, if the amount of root extract is less than 2 parts by weight of the amount is too small, the skin protection and skin regeneration effect may be inadequate, when used in excess of 15 parts by weight may cause skin trouble rather than due to overuse.

The anti-aging cosmetics of the present invention may vary in composition and composition ratio of the cosmetics, which are mixed with Root Root Extract, depending on the formulation.

As a preferred example, when the anti-aging cosmetics are cream formulations, the cosmetics may include an aqueous phase, an oil phase, a thickener and a neutralizing agent, and preferably 45 to 54% by weight, 20 to 25% by weight of an oil phase, a thickener. 20 to 30% by weight and the balance of neutralizing agent may be included, more preferably 47 to 50% by weight of water phase, 22.5 to 25% by weight of oil phase, 23.5 to 27.5% by weight of thickener and the balance of neutralizing agent.

And, in the case of a cream formulation, for example, a preferred embodiment of each of an aqueous phase, an oil phase, a thickener and a neutralizing agent, those having a composition as shown in Table 1 may be used. In Table 1 below, the water phase is based on 100 parts by weight of water, the oil phase is based on 100 parts by weight of shea butter, and the thickener and neutralizing agent are expressed in parts by weight based on 100 parts by weight of water.

division ingredient Usage (part by weight) Awards water 100 Disodium ID 0.05 to 0.35, preferably 0.12 to 0.20 Xanthan Gum 0.05 to 0.20, preferably 0.06 to 0.15 Sodium hyaluronate 0.01 to 0.15, preferably 0.03 to 0.10 Aloe Vera Leaf Powder 0.50 to 1.8, preferably 0.70 to 1.25 glycerin 7.0 to 12.5, preferably 8.0 to 10.5 Butylene Glycol 25 to 35, preferably 28 to 32 Biosaccharide Gum-1 0.10 to 0.50, preferably 0.15 to 0.35 Paid Shea butter 100 Stearic acid 25 to 55, preferably 32 to 48 Cetyl alcohol 50 to 70, preferably 55 to 65 Fiji-40 Stearate 20 to 40, preferably 25 to 35 Bis-pig-18-methyletherdimethylsilane 80 to 120, preferably 90 to 115 Glyceryl Stearate 20 to 40, preferably 25 to 35 Olive oil 1 to 10, preferably 2.5 to 6.5 Tocopheryl Acetate 10 to 25, preferably 12 to 18 Squalene 15 to 40, preferably 20 to 35 Sunflower Seed Oil 15 to 40, preferably 20 to 35 Grape seed oil 15 to 40, preferably 20 to 35 Sweet Almond Oil 40 to 70, preferably 45 to 60 Camellia Seed Oil 1 to 10, preferably 3.0 to 6.0 Gardenia oil 0.5 to 5, preferably 1.5 to 4 Cetylethylhexanoate 170 to 250, preferably 185 to 220 Cyclomethicone 280 to 370, preferably 300 to 340 Thickener water 100 Acrylate / C10-30 Alkylacrylate Crosspolymer 0.1 to 2.5, preferably 0.6 to 1.5 corrector water 100 Arginine 10 to 30, preferably 12 to 25

In addition, when the anti-aging cosmetics is a toner formulation, the cosmetic may include an aqueous phase, a solubilizer, a first thickener, a second thickener and a solubilizer, preferably 75 to 85% by weight of the aqueous phase, solubilizer 2.5 to 7 It may include a weight percent, 4.0 to 7.5% by weight of the first thickener, 6.0 to 10.0% by weight of the second thickener and the remaining amount of the solubilizer, more preferably 77 to 82.5% by weight of water, 3.0 to 6.5% by weight of the solubilizer It may include 4.5 to 6.5% by weight of the first thickener, 7.0 to 9.0% by weight of the second thickener and a balance of the solubilizer.

In the case of the toner formulation, a preferred embodiment of each of an aqueous phase, a dissolving agent, a first thickening agent, a second thickening agent, and a solubilizing agent may be used, having a composition as shown in Table 2 below. In Table 2, the water phase is based on 100 parts by weight of water, the solubilizer is based on 100 parts by weight of glycerol-26, the first thickener and the second thickener are based on 100 parts by weight of water, and the solubilizer is dipropylene glycol 100. The amount of other components based on parts by weight is expressed in parts by weight.

division ingredient Usage (part by weight) Awards water 100 Disodium ID 0.01 to 1, preferably 0.01 to 0.06 Allantoin 0.05 to 0.5, preferably 0.05 to 0.25 Arginine 0.05 to 0.5, preferably 0.10 to 0.20 Virginia Pond Water 0.40 to 2.0, preferably 0.5 to 1.5 Galactomyces fermentation filtrate 0.30 to 2.5, preferably 0.4 to 1.7 Methylgluses-20 0.10 to 2.0, preferably 0.4 to 1.2 Niacinamide 2.0 to 5.0, preferably 2.5 to 4.5 Butylene Glycol 7.4 to 13.0, preferably 8.0 to 11.5 Glycerin / Glyceryl Acrylate / Acrylic Acid Copolymer / Propylene Glycol (Hydrogel CGKC) 0.40 to 2.0, preferably 0.5 to 1.5 Sodium hyaluronate 0.40 to 2.0, preferably 0.5 to 1.5 Solvent Glyceres-26 100 1,2-hexanediol / caprylyl glycol 20 to 35, preferably 23 to 30 1st increment water 100 Hydroxyethyl cellulose 0.2 to 2.5, preferably 0.7 to 1.5 Secondary thickener water 100 Acrylate / C10-30 Alkylacrylate Crosspolymer 0.2 to 4.5, preferably 0.5 to 2.5 Solubilizer Dipropylene Glycol 100 Fiji-60 Hydrogenated Castor Oil 4 to 10, preferably 5.0 to 9.5 Polysorbate 80 0.5 to 5, preferably 0.5 to 4 Tocopheryl Acetate 0.5 to 8, preferably 1.0 to 4.5

In addition, when the anti-aging cosmetics is an emulsion formulation, the cosmetics may include an aqueous phase, an oil phase, a first thickener, a second thickener and a neutralizing agent, preferably 75 to 85% by weight of the aqueous phase, 5 to 10% by weight of the oil phase. %, The first thickener 0.1 to 2.0% by weight, the second thickener 8.0 to 15.0% by weight and the balance of the neutralizing agent, more preferably 76 to 80% by weight, 6.5 to 8.5% by oil phase, agent It may include 0.2 to 1.0 weight percent of a thickener, 10.5 to 13.8 weight percent of a second thickener, and a balance of neutralizing agent.

And, in the case of the emulsion formulation, a preferred embodiment of each of the water phase, oil phase, the first thickener, the second thickener and the neutralizing agent, for example, those having a composition as shown in Table 3 can be used. In Table 3 below, the water phase is based on 100 parts by weight of water, and the oil phase is based on 100 parts by weight of cetyl alcohol, and the first thickener, the second thickener, and the neutralizing agent are expressed in parts by weight based on 100 parts by weight of water. .

division ingredient Usage (part by weight) Awards water 100 Betaine 0.05 to 0.50, preferably 0.10 to 0.35 Disodium ID 0.01 to 0.20, preferably 0.02 to 0.10 Glyceres-26 2 to 8, preferably 4.0 to 6.5 Polyglyceryl-3PCA 0.05 to 0.50, preferably 0.10 to 0.35 Butylene Glycol 5 to 15, preferably 8 to 13 Niacinamide 1 to 6, preferably 2.0 to 5.0 Methylgluses-20 0.2 to 2.0, preferably 0.5 to 1.70 Sodium hyaluronate 0.01 to 1, preferably 0.03 to 0.20 Dipropylene Glycol 2 to 8, preferably 2.5 to 6.5 1,2-hexanediol / caprylyl glycol 0.5 to 4, preferably 1.0 to 2.5 Paid Cetyl alcohol 100 Glyceryl Stearate / Pig-100 Stearate 45 to 80, preferably 55 to 70 Polysorbate 60 15 to 40, preferably 20 to 32 Glyceryl Stearate 25-50, preferably 32-42 Cetylethylhexanoate 200 to 300, preferably 220 to 280 Tocopheryl Acetate 5 to 20, preferably 8 to 16 Cyclomethicone 200 to 300, preferably 230 to 275 Dimethicone and Dimethicone
Vinyl Dimethicone Cross Polymer 120 to 250, preferably 150 to 210 1st increment Ammonium Acryloyl-Dimethyl Taurate / V-Picopolymer 100 Secondary thickener water 100 Acrylate / C10-30 Alkyl acrylate cross polymer 0.1 to 5, preferably 0.5 to 2.5 corrector water 100 Arginine 2 to 12, preferably 4 to 10

In addition, when the anti-aging cosmetics is an essence formulation, the cosmetic may include an aqueous phase, a first thickener, a second thickener, a solubilizing agent and a neutralizing agent, preferably 50 to 70% by weight of the aqueous phase, the first thickener 5 to 15% by weight, 15 to 25% by weight of the second thickener, 1 to 10% by weight of the solubilizer and the remaining amount of neutralizing agent, more preferably 58 to 63% by weight of the water phase, 8.5 to 1% thickener 12.5% by weight, 18.5-22.5% by weight of the second thickener, 3-5% by weight of the solubilizer and the balance of the neutralizing agent.

And, in the case of an essence formulation, for example, a preferred embodiment of each of the aqueous phase, the first thickener, the second thickener, the solubilizer and the neutralizing agent, those having a composition as shown in Table 4 may be used. In Table 4, the aqueous phase, the first thickener, the second thickener, and the neutralizing agent are expressed in parts by weight based on 100 parts by weight of water and the solubilizing agent based on 100 parts by weight of dipropylene glycol.

division ingredient Usage (part by weight) Awards water 100 Disodium ID 0.010 to 0.150, preferably 0.02 to 0.10 Betaine 0.1 to 0.80, preferably 0.15 to 0.45 glycerin 6-15 parts by weight, preferably 8.0-12.5 Butylene Glycol 8 to 20 parts by weight, preferably 10 to 15 Polyglyceryl-3PCA 0.1 to 2, preferably 0.5 to 1.5 Methylgluses-20 1.5 to 5 parts by weight, preferably 1.8 to 3.2 Glycerin / Glyceryl Acrylate / Acrylic Acid Copolymer / Propylene Glycol 2.0 to 7.0, preferably 2.5 to 5.0 Niacinamide 2.0 to 10.0, preferably 3.5 to 8.0 Sodium hyaluronate 4 to 12, preferably 5.0 to 8.8 1st increment water 100 Hydroxyethyl cellulose 5 to 15, preferably 8.5 to 14 Secondary thickener water 100 Acrylate / C10-30 Alkylacrylate Crosspolymer 0.5 to 5, preferably 0.5 to 3 Solubilizer Dipropylene Glycol 100 Fiji-60 Hydrogenated Castor Oil 4.0 to 10.0, preferably 4.5 to 8.5 Polysorbate 80 0.5 to 5.0, preferably 1.0 to 2.7 1,2-hexanediol / caprylyl glycol 3 to 10, preferably 5 to 9 Tocopheryl Acetate 0.1 to 5, preferably 1 to 4 corrector water 100 Arginine 5 to 20, preferably 6 to 15

The anti-aging cosmetics of the present invention prepared by the above method is excellent in the Nrf2 expression effect, excellent collagen and matrix metalloproteinase-1 (MMP-1) control bar, prevents skin aging, improves skin wrinkles and elasticity, skin regeneration effect great.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are provided to help the understanding of the present invention, and the scope of the present invention should not be narrowly interpreted by the following examples.

EXAMPLE

Example 1 Preparation of Root Root Butylene Glycol Extract

After washing, dry roots were prepared by cutting to a certain size.

Next, after adding a circular flask butylene glycol, 10 parts by weight of the cut dried roots with respect to 100 parts by weight of the butylene glycol was added and stirred.

Next, after the circular flask was put into the heating mantle, the EYEA (cooler) machine was connected and operated. And it left for 2 hours at 205 degreeC, and obtained the extract.

Next, after inserting the funnel funnel connected to the decompression equipment, the filter paper was put on the funnel. After the decompression equipment was operated, the extract was poured on the funnel to separate the primary extract and the residue.

In addition, since the viscosity and a large amount of the suspended solids were not filtered smoothly, secondary filtration was performed in the same manner.

Next, with respect to 100 parts by weight of the secondary filtration extract, 0.5 parts by weight of phenoxyethanol as a preservative was mixed and stirred in the same manner as the secondary filtration to obtain a final root root extract.

Figure 1 shows a schematic process diagram of the preparation of the root root butylene glycol extract of the present invention.

Comparative Example 1 to Comparative Example 2

칡 Root butylene glycol extracts were prepared in the same manner as in Example 1, but when the extracts 1 and 2 were prepared, 칡 extracts were obtained at the temperature conditions as shown in Table 5, not at 205 ° C., and Comparative Examples 1 to 2 were obtained. Was carried out.

division Example 1 Comparative Example 1 Comparative Example 2 Extraction temperature 205 ℃ 170 ℃ 230 ℃

Experimental Example 1: Determination of Nrf2 expression and collagen expression control effect of 칡 extract

The Nrf2 expression and collagen (COL1A1) regulatory effects of the extract were determined by the expression level at the protein level. Nrf2 is a transcription factor that regulates the expression of the antioxidant enzymes HO-1, NQO-1, etc. It can be said that it maintains the intrinsic antioxidant defense mechanism of the substance depending on the expression of Nrf2.

Nrf2 expression and collagen expression regulation effect measurement Human fibroblasts were cultured in a culture dish and then treated with UVB to simulate the actual photoaging environment. The control group (con) was not treated at all, the negative control group (Non) is the experimental group treated with UVB only. The Q group was treated with 10 uM of quercetin (quercetin), which is widely known as a natural antioxidant as a positive control, and as a test group, 농도 extract was treated at concentrations of 25, 50, and 100 ug / ml, respectively.

In the experiment, the cells were recovered and the proteins were extracted and Western blotting was performed using protein antibodies of Nrf2, collagen, MMP-1, and HO-1, respectively, and the expression of these proteins was measured. Is shown in FIG. 2. In addition, expression of HO-1, a lower antioxidant enzyme expressed by Nrf2, was also observed.

In addition, the protein expression experiment was normalized to the expression level of the actin (actin), a reference protein, using the Image J program to measure the relative expression amount is shown in Figure 3 the results.

As can be seen in Figure 3, Nrf2, an antioxidant enzyme regulator, showed an increased expression level compared to quercetin, a positive control, and it was observed that the expression amount was changed in a concentration-dependent manner (Fig. 3A).

In addition, it was confirmed that the expression of the antioxidant enzyme HO-1 regulated by Nrf2 was expressed in a concentration-dependent manner similar to the expression pattern of Nrf2 (FIG. 3B). In the case of collagen, which is an important protein for maintaining the structure of the skin, the amount of expression was changed depending on the treatment concentration of the extract 칡 (FIG. 3C).

Therefore, in this experiment it was confirmed that the extract extracts have a concentration-dependent effect on the expression of various antioxidant factors and skin component proteins.

Preparation Example 1 Preparation of Cream Formulation Cosmetics

To 100 parts by weight of the cosmetic composition having the composition and the composition ratio of Table 6, 5.3 parts by weight of the Root root extract prepared in Example 1 was mixed and stirred to prepare a cream formulation cosmetic.

The photograph of the manufactured cosmetics is shown in FIG.

division Ingredients (brand name) Parts by weight weight% Awards water 100 48.87 Disodium ID (EDTA-2Na) 0.15 Xanthan Gum (keltrol-F) 0.09 Sodium hyaluronic acid 0.06 Aloe vera powder 0.92 glycerin 9.1 Butylene glycol (1, 3- butylene glycol) 30.35 Biosaccharide Gum-1 (Fucogel 100) 0.25 Paid Shea butter 100 23.95 Stearic Acid (Emersol7036) 40 Cetyl Alcohol (kalcohol 6870P) 60 Fiji-40 stearate (Myrj-52S) 30 Bis-Pig-18-Methyletherdimethylsilane (Dow Corning 2501 Wax) 100 Glyceryl Stearate 30 Olive oil 5 Tocopheryl Acetate (Vitamin E Acetate) 15 Squalene 25 Sunflower Seed Oil 25 Grape seed oil 25 Sweet Almond Oil 50 Camellia Seed Oil 5 Gardenia oil (Gardenia yellow OL M2649) 2.5 Cetylethylhexanoate (paester 9168) 200 Cyclomethicone (SF00152) 325 Thickener water 100 25.26 Acrylate / C10-30 Alkylacrylate Crosspolymer (cabopol-ETD2020) 1.01 corrector water 100 Remainder
Remaining amount Arginine (L-arginine) 16 Total weight 100

Preparation Example 2 Preparation of Toner Formulation Cosmetics

Toner formulation cosmetics were prepared by mixing and stirring 5.3 parts by weight of Root Root Extract prepared in Example 1 with respect to 100 parts by weight of cosmetics having the composition and composition ratio of Table 7.

The photograph of the manufactured cosmetics is shown in FIG.

division ingredient Parts by weight weight% Awards water 100 79.32 Disodium ID (EDTA-2Na) 0.03 Allantoin 0.15 Arginine 0.12 Virginia Pond Water Flower (Witch Hazel Water) 0.77 Galactomyces fermentation filtrate (galactomyces) 0.77 Methylgluses-20 (glucam E-20) 0.77 Niacinamide 3.09 Butylene glycol (1, 3- butylene glycol) 9.28 Glycerin / Glyceryl Acrylate / Acrylic Acid Copolymer / Propylene Glycol (Hydrogel CGKC) 0.77 Sodium hyaluronate 0.77 Solvent Glycereth-26 100 4 1,2-hexanediol / caprylyl glycol (ricomildiol) 26.67 1st increment water 100 5.26 Hydroxyethyl Cellulose (Natrasol-250HR) 1.01 Secondary thickener water 100 8.42 Acrylate / C10-30 Alkylacrylate Crosspolymer (cabopol-ETD2020) 1.01 Solubilizer Dipropylene Glycol (DPG-FC) 100 Remainder
Remaining amount Fiji-60 Hydrogenated Castor Oil (HCO-60) 6.67 Polysorbate 80 (Rheodol TW-O120V) 1.67 Tocopheryl Acetate (Vitamin E Acetate) 1.67 Total weight 100

Preparation Example 3 Preparation of Emulsion Formulation Cosmetics

To 100 parts by weight of the cosmetic composition having the composition and composition ratio of Table 8, 5.3 parts by weight of the Root root extract prepared in Example 1 was mixed and stirred to prepare an emulsion formulation cosmetic.

The photograph of the manufactured cosmetics is shown in FIG.

division ingredient Parts by weight weight% Awards water 100 77.51 Betaine 0.17 Disodium ID 0.03 Glyceres-26 5.08 Polyglyceryl-3PCA 0.17 Butylene glycol (1, 3- butylene glycol) 10.16 Niacinamide 3.39 Methylgluses-20 (gulcam E-20) 0.85 Sodium hyaluronate (hyaluronic acid powder) 0.08 Dipropylene Glycol (DPG-PC) 3.39 1,2-hexanediol / caprylyl glycol (Ricomildiol) 1.35 Paid Cetyl Alcohol (kalcohol 6870P) 100 7.79 Glyceryl Stearate / Pig-100 Stearate (Aracel-165) 62.5 Polysorbate 60 (Rheodol TW-S120V) 25 Glyceryl Stearate (CEH) 37.5 Cetylethylhexanoate 250 Tocopheryl Acetate 12.5 Cyclomethicone (SF00152) 250 Dimethicone & Dimethicone / Vinyl Dimethicone Crosspolymer (Serasilk EL61) 187.5 1st increment Ammonium Acryloyldimethyl Taurate / V-Picopolymer (Aristoflex AVC) 100 0.37 Secondary thickener water 100 12.58 Acrylate / C10-30 Alkylacrylate Crosspolymer (cabopol-ETD2020) 1.01 corrector water 100 Remainder
Remaining amount Arginine 6 Total weight 100

Preparation Example 4 Preparation of Essence Formulation Cosmetics

To 100 parts by weight of the cosmetic composition having the composition and the composition ratio of Table 9, 5.3 parts by weight of the Root root extract prepared in Example 1 was mixed and stirred to prepare an essence formulation cosmetic.

The photograph of the manufactured cosmetics is shown in FIG.

division ingredient Parts by weight weight% Awards water 100 61 Disodium ID 0.047 Betaine 0.23 glycerin 11.72 Butylene glycol (1, 3- butylene glycol) 11.72 Polyglyceryl-3PCA 0.7 Methylgluses-20 (gulcam E-20) 2.34 Glycerin / Glyceryl Acrylate / Acrylic Acid Copolymer / Propylene Glycol (Hydrogel CGKC) 3.51 Niacinamide 4.69 Sodium hyaluronate (hyaluronic acid powder) 7.03 1st increment water 100 11 Hydroxyethyl Cellulose (Natrosol-250HR) 10.1 Secondary thickener water 100 21 Acrylate / C10-30 Alkylacrylate Crosspolymer (cabopol-ETD2020) 1.01 Solubilizer Dipropylene Glycol 100 4 Fiji-60 Hydrogenated Castor Oil (HCO-60) 6.67 Polysorbate 80 (Rheodol TWO 120V) 1.67 1,2-hexanediol / caprylyl glycol (ricomildiol) 6.67 Tocopheryl Acetate (Vitamin E Acetate) 1.67 corrector water 100 Remainder
Remaining amount Arginine 10 Total weight 100

Experimental Example 2: Evaluation of Skin Safety

Skin safety evaluation was performed using the cream formulation cosmetic prepared in Preparation Example 1.

Evaluation period was from September 10 to October 1, 2018, the evaluation method was carried out through a 24-hour closed patch test, the specific method is shown in Table 10 below.

24 hours closed cloth
1. Subjects: 33 adult men and women were selected (within 10% dropout rate, 33 final women completed, average 44.03 ± 7.89 years old)
2. Patch area: Remove the sample after 24 hours by applying the sample to the back of the subject
3. Observation of test site: Observation of skin reactions on 5-point scale (erythema, skin, edema) after 30 minutes, 24 hours, 48 hours after patch removal.
4. Determination of test site stimulus
Skin response was evaluated according to the criteria of the Draize Dermal Irritation Scoring System [Figure 1].
After calculating the Mean Irritation Index (MII) according to the formula for calculating the average skin irritation, the stimulation group was determined according to the skin primary stimulation test result determination table reflecting the stimulation classification criteria of the Environmental Protection Agency (EPA) Standard Evaluation Procedure Dermal Classification System. TABLE 11
5. Test methods and evaluation: Evaluation based on the regulations on functional cosmetics examination, PCPC Safety Evaluation Guidelines, Draize Dermal Irritation Scoring System and EPA Standard Evaluation Procedure Dermal Classification System.
6. Evaluation of Skin Adverse Events: Evaluation of skin adverse events by researchers and subjects.

[Figure 1]

Grade M.I.I. Negligible 0.00≤M.I.I <0.50 Slight 0.50≤ M.I.I <2.00 Moderate 2.00≤ M.I.I <5.00 Strong 5.00≤ M.I.I <8.00

The skin patch test results are shown in Table 12 below.

Number of respondents Skin irritation degree Average skin
Stimulus After 30 minutes After 24 hours After 48 hours One 2 3 4 One 2 3 4 One 2 3 4 - Erythema 7 4 One - - 4 - - - One - - - 0.11 edema 0 - - - - - - - - - - - -

Results of adverse events assessed by the investigator and subjects for 33 subjects who completed the final trial. During the research institute, the samples did not show any specific skin adverse events other than those expected or likely to be involved in the patch test.

Claims (10)

delete delete delete delete In the cream formulation cosmetic manufacturing method, it is prepared by mixing 2-15 parts by weight of Root Root Extract, based on 100 parts by weight of the cosmetic of the cream formulation,
The Root Root Extract
With respect to 100 parts by weight of butylene glycol at a concentration of 95.0 to 99.99% by volume, after preparing a mixed solution mixed with 5-15 parts by weight of roots, the mixed solution was left at 180 to 220 ° C. for 1 to 4 hours to extract the extract. Obtaining and then filtering the first, the first step of separating the primary extract and the residue;
Performing a second filtration of the primary extract; And
It was prepared by performing a process comprising; three steps of filtration three times the mixed solution of the secondary filtration extracts and preservatives;
Cosmetics of the cream formulations include an aqueous phase, an oil phase, a thickener and a neutralizing agent,
The aqueous phase includes water, disodium ethane, xanthan gum, sodium hyaluronate, aloe vera leaf powder, glycerine, butylene glycol and biosaccharide gum-1,
The oil phase is glycerylstearate, stearic acid, cetyl alcohol, cYG-40 stearate, bis-PYG-18 methyl ether dimethyl silane, shea butter butter), cetearyl alcohol, olive oil, tocopheryl acetate, squalene (squalene), sunflower seed oil, grape seed oil, sweet almond oil, camellia seed oil, gardenia oil, cetylethylhexanoate and cyclomethicone Include,
The thickener comprises water and an acrylate / C 10-30 alkylacrylate crosspolymer,
The neutralizing agent is a method for producing an antioxidant and skin wrinkle improvement cosmetics comprising water and L-arginine (L-arginine).
In the toner formulation cosmetic manufacturing method, it is prepared by mixing 2 to 15 parts by weight of Root root extract with respect to 100 parts by weight of cosmetics of the toner formulation,
The Root Root Extract
With respect to 100 parts by weight of butylene glycol at a concentration of 95.0 to 99.99% by volume, after preparing a mixed solution mixed with 5-15 parts by weight of roots, the mixed solution was left at 180 to 220 ° C. for 1 to 4 hours to extract the extract. Obtaining and then filtering the first, the first step of separating the primary extract and the residue;
Performing a second filtration of the primary extract; And
It was prepared by performing a process comprising; three steps of filtration three times the mixed solution of the secondary filtration extracts and preservatives;
The cosmetic of the toner formulation includes an aqueous phase, a dissolving agent, a first thickener, a second thickener and a solubilizer,
The water phase is water, disodium IDTA, allantoin, allginin, arginine, Virginia plunger water, galactomises fermentation filtrate, methylgluses-20, niacinamide, butyl Len glycol, glycerin / glyceryl acrylate / acrylic acid copolymer, and propylene glycol mixture and sodium hyaluronate,
The solubilizer includes glycereth-26 and 1,2-hexanediol / caprylyl glycol,
The first thickener includes water and hydroxyethyl cellulose aqueous solution,
The second thickener comprises water and an aqueous solution of acrylate / C 10-30 alkyl acrylate crosspolymer,
The solubilizer is a method for producing an anti-oxidant and anti-wrinkle cosmetic comprising dipropylene glycol, PIG-60 hydrogenated castor oil, polysorbate 80 and tocopheryl acetate.
In the emulsion formulation cosmetic manufacturing method, it is prepared by mixing 2 ~ 15 parts by weight of Root Root Extract, based on 100 parts by weight of cosmetics of the emulsion formulation,
The Root Root Extract
With respect to 100 parts by weight of butylene glycol at a concentration of 95.0 to 99.99% by volume, after preparing a mixed solution mixed with 5-15 parts by weight of roots, the mixed solution was left at 180 to 220 ° C. for 1 to 4 hours to extract the extract. Obtaining and then filtering the first, the first step of separating the primary extract and the residue;
Performing a second filtration of the primary extract; And
It was prepared by performing a process comprising; three steps of filtration three times the mixed solution of the secondary filtration extracts and preservatives;
The cosmetic formulation of the emulsion formulation includes an aqueous phase, an oil phase, a first thickener, a second thickener and a neutralizing agent,
The aqueous phase is water, Betaine, Disodium IDTA, Glycereth-26, Polyglyceryl-3P, Butylene glycol, Niacinamide, Methyl Gluses-20, sodium hyaluronate, dipropylene glycol and 1,2-hexanediol and caprylyl glycol mixture,
The oil phase is cetyl alcohol, glyceryl stearate and PG-100 stearate mixture, polysorbate 60, glyceryl stearate, cetyl ethyl hexanoate, tocopheryl acetate, cyclomethicone and dimethicone & dimethicone / Vinyl dimethicone cross polymer,
The first thickener comprises ammoniumacryloyldimethyltaurate / VPicopolymer,
The second thickener comprises water and an acrylate / C 10-30 alkylacrylate crosspolymer,
The neutralizing agent is a method for producing an antioxidant and skin wrinkle improvement cosmetics comprising water and arginine.
Essence (essence) formulation cosmetic manufacturing method, based on 100 parts by weight of cosmetics of the essence formulation, it is prepared by mixing 2 to 15 parts by weight of root extract,
The Root Root Extract
With respect to 100 parts by weight of butylene glycol at a concentration of 95.0 to 99.99% by volume, after preparing a mixed solution mixed with 5-15 parts by weight of roots, the mixed solution was left at 180 to 220 ° C. for 1 to 4 hours to extract the extract. Obtaining and then filtering the first, the first step of separating the primary extract and the residue;
Performing a second filtration of the primary extract; And
It was prepared by performing a process comprising; three steps of filtration three times the mixed solution of the secondary filtration extracts and preservatives;
Cosmetics of the essence formulations include an aqueous phase, a first thickener, a second thickener, a solubilizer and a neutralizer,
The aqueous phase may be water, disodium ethane, betaine, glycerin, butylene glycol, polyglyceryl-3PCA, methylgluses-20, glycerin / glycerylacrylate / acrylic acid copolymer and Propylene glycol mixture, niacinamide and sodium hyaluronate,
The first thickener includes water and hydroxyethyl cellulose,
The second thickener comprises water and an acrylate / C 10-30 alkylacrylate crosspolymer,
The solubilizer includes dipropylene glycol, Fiji-60 hydrogenated castor oil, polysorbate 80, 1,2-hexanediol and caprylyl glycol mixture and tocopheryl acetate,
The neutralizing agent is a method for producing an antioxidant and skin wrinkle improvement cosmetics comprising water and arginine.
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