KR20130029290A - Cosmetic compostion for skin cell regeneration - Google Patents

Abstract

PURPOSE: A cosmetic composition containing an Osmunda japonica Thunb. extract, a Nelumbo nucifera Gaertn. extract, and an Ulmus davidiana var. japonica NAKAI is provided to effectively regenerate skin and to ensure anti-inflammation with safety and less irritation. CONSTITUTION: A cosmetic composition for skin regeneration contains one or more plant extracts selected from the group consisting of Osmunda japonica Thunb., Nelumbo nucifera Gaertn. and Ulmus davidiana var. japonica NAKAI. The composition contains 0.001-10 wt% of Osmunda japonica Thunb., 0.001-10 wt% of Nelumbo nucifera Gaertn., and 0.001-10 wt% of Ulmus davidiana var. japonica NAKAI. The plant extract is prepared using water, organic solvent or a mixture thereof.

Description

Cosmetic composition for skin regeneration {Cosmetic compostion for skin cell regeneration}

The present invention relates to a cosmetic composition for skin regeneration, and more particularly to a cosmetic composition for skin regeneration containing at least one plant extract selected from the group consisting of ferns, lotus and elm.

Causes of skin aging include internal aging and external aging. Internal aging is caused by a decrease in the secretion of various hormones that regulate metabolism with age, deterioration of immune cell function and cell activity, and external aging is caused by free radicals caused by external factors such as UV and environmental pollution. And active toxic oxygen.

When skin aging occurs, not only decreases the thickness and elasticity of the skin, increases wrinkles, but also the color of the skin is poor, skin problems frequently occur, and blemishes, freckles, and mushrooms also increase.

In vivo, the synthesis and degradation of extracellular substrates such as collagen and elastin are properly regulated, but their synthesis decreases as aging progresses, and matrix metalloproteinase (MMP), an enzyme that breaks down collagen, breaks down elastin. Expression of the enzyme elastinase (elastase) is promoted to reduce the elasticity of the skin and wrinkles are formed.

In addition, prostaglandin E2 (Prostaglandin E2, Prostaglandin E2, Prostaglandin E2, Protetaglandin E2 (Proxtaglandin E2) due to the increase in cyclooxygenase-2 (COX-2), an enzyme that produces inflammatory cytokines in skin cells Biosynthesis of PGE2) is increased, and nitric oxide (NO) production is known to be increased by inducible nitric oxide synthase (iNOS). These inflammatory reactions result in increased biosynthesis of matrix metalloproteinase (MMP) resulting in collagen degradation.

Adenosine, retinoic acid, and the like are known to be effective in improving skin wrinkles, but adenosine is ineffective in clinical practice, retinoic acid can be used in women of childbearing potential, and erythema and the like are highly feared.

Skin protects against various stimuli from the outside and prevents excessive release of moisture through the skin, and this protection function can be maintained only when the stratum corneum, which is the outermost layer, is normally formed. The stratum corneum is formed from keratinocytes and forms the stratum corneum while producing natural moisturizing factor (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids). It has a function as a skin barrier.

However, the stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air and pollutants, and endogenous diseases such as atopic or aged skin.

Accordingly, the present inventors found that the composition containing one or more plant extracts selected from the group consisting of ferns, lotuses and elm improves skin elasticity and wrinkles by promoting the production of collagen and improves skin moisturizing, anti-inflammatory and skin regeneration It was confirmed that the effect is excellent.

Accordingly, the present invention provides a cosmetic composition for skin regeneration containing at least one plant extract selected from the group consisting of fern, lotus and elm, which improve skin elasticity and wrinkles, and are effective for anti-inflammatory and skin regeneration. There is this.

In one embodiment of the present invention, there is provided a cosmetic composition for skin regeneration containing at least one plant extract selected from the group consisting of ferns, lotus and elm.

The fern is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition, the lotus is contained in an amount of 0.001 to 10% by weight relative to the total weight of the composition, the elm is 0.001 to 10% by weight relative to the total weight of the composition It is contained in the amount of.

In addition, the fern, lotus or elm wood promotes the production of collagen and moisturizes the skin.

In one embodiment of the present invention, the cosmetic composition is selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, shampoo, oil-in-water (O / W) type and water-in-oil (W / O) type formulation 1 or more types.

In one embodiment of the present invention, the one or more plant extracts selected from the group consisting of ferns, lotus and elm are extracted with water, an organic solvent or a mixture thereof, and the organic solvent is anhydrous or contains 1 to 4 carbon atoms. At least one selected from the group consisting of methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.

The composition according to the present invention is effective in improving skin elasticity and wrinkles by promoting collagen production and improving skin moisturizing effect, effective in anti-inflammatory and skin regeneration, is less irritating to the skin and is safe for human body including long-term natural plant. There is no side effect even if used.

The present invention is described in more detail as follows.

Fern, lotus or elm, which are the active ingredients of the cosmetic composition of the present invention, have the following characteristics.

Gobi ( Osmunda japonica Thunb.) Is a plant belonging to the family of ferns. It looks similar to ferns, but unlike ferns, perennials are ferns in the fern family. It is a large plant about 1m in height. Young leaves are covered with white down, but grow reddish brown as they grow. In early spring, raw leaves come out first, followed by nutritious leaves, break young shoots, and eat herbs or soup. Spores are formed in March and spores are held in May. Young shoots are eaten as herbs or as ingredients of soup. In oriental medicine, root stem is used as a medicinal herb, and it is effective for fever and skin rash caused by cold, remove parasites, and hemostatic effect. In the private sector, they are dug up and dried in spring and summer, and stems and leaves are used for sore throats and roots are used as diuretics.

Lotus (Nelumbo nucifera Gaertn . ) Is a plant belonging to the water lily family and is a perennial herb that is grown in the sub-central region of Korea. It grows in wetlands or ponds near villages. It grows about 1m in height, and leaves come from the roots. Its diameter is about 40cm and it is raised above the water in the shape of a shield. Fruits are black, oval, and 2cm long. The leaves are said to be used as astringents or hemostatic agents or for bladder treatment in the private sector. Underground stems are called lotus root and are high in vitamins and minerals. Root lotus root is used a lot in general cooking. Rhizome and berries are also used for medicinal purposes, and in oriental medicine, sore throat, rhinitis, gynecological treatment.

Elm ( Ulmus) davidiana there is . japonica NAKAI ) is a plant belonging to the elm family, also known as spring or crayfish. Its height is 20m and its diameter is 60cm, the bark is gray brown, and the twig has reddish brown short hairs. The leaves are alternately oval, with the wide eggs upside down or the eggs upside down, with a sharp pointed tip and a leaf tooth on the edge of the leaf. Leaf front is coarse with short, coarse hair on back vein. In the spring, young leaves are edible, and in Chinese medicine, the bark is used as a medicinal herb called dermis or dermis.

The at least one plant extract selected from the group consisting of ferns, lotus and elm is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition, when the extract is contained in less than 0.001% by weight of the skin regeneration effect It may be insignificant, if it is included in more than 10% by weight, the stability of the formulation may be lowered or the fragrance may not be good when manufacturing cosmetics.

The fern, lotus and elm extracts are pulverized ferns, lotus and elm, respectively, and extracted by adding a hydrophilic organic solvent such as methanol, ethanol, or a mixed solution of hydrophilic organic solvent and water as an extractant and immersing for a predetermined time. The solution obtained after stirring at intervals can be obtained according to the usual natural product extraction method by filtration and recovery.

Preferred extraction solvents include one or more pure solvents selected from the group consisting of anhydrous or containing methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol, and water. Combined mixed solvents may be used, and the amount of extractant added to the plant material may be about 1 to 50 times the total volume of the plant material.

More preferably, as the extraction solvent of the present invention, it is preferable to use one pure solvent selected from anhydrous methanol, hydrous methanol, anhydrous ethanol and hydrous ethanol, or a mixed solvent of one of the methanol and one of the ethanol.

At this time, the addition amount of the extraction solvent is preferably 5 to 20 ml per 1 g dry weight of the fern, lotus and elm. The conventional solvent extraction method, such as extraction by immersion at room temperature for 1 to 7 days or extraction for about 1 to 5 hours at a heating condition of 50 to 95 ℃.

The cosmetic composition is at least one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, shampoo, oil-in-water (O / W) type and water-in-oil (W / O) type.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, these Examples and Experimental Examples are not intended to limit the scope of the present invention only to aid the understanding of the present invention.

Manufacturing example  1. Fern Extract Manufacturing

After pulverizing the fertilizer and powdering it, 80% ethanol was added to the volume of about 2 times the volume of the high fertilizer and cooled for 3 days, and then all the solutions were collected and filtered through a filter paper. The solvent was removed using an evaporator and then dried in a freeze dryer. Dried for at least 48 hours to form a powder.

Manufacturing example  2. Lotus extract manufacture

After crushing and pulverizing the lotus, 80% ethanol was added to twice the volume of lotus and chilled for 3 days, and then the solution was collected and filtered through a filter paper. The solvent was removed using an evaporator and then dried in a freeze dryer. Dried for at least 48 hours to form a powder.

Manufacturing example  3. Elm Extract Manufacturer

After crushing and elmating the elm tree, 80% ethanol is added to the volume of about twice the amount of elm and cooled for 3 days, and then all the solutions are collected and filtered through a filter paper. Dried at, and dried for at least 48 hours to form a powder.

Experimental Example  1. Type I Procollagen Promoting Experiment

Human normal fibroblasts were incubated for 24 hours in a 24-well plate at a concentration of 5 × 10 ⁴ , and then replaced with a medium containing fern, lotus, and elm extract and treated for 24 hours. TGF-β (Human Transforming Growth Factor-β1, Roche Co.) was used as a positive control. The amount of type I procollagen produced by harvesting cells on the third day of culture was quantified by ELISA. The amount of procollagen of the control group without the extract was set to 0, and the positive control group was set to 100, and the comparison with each measured value was calculated. The results are shown in Table 1 below.

Type I procollagen biosynthesis (%) Control group (untreated group) 0 TGF-β (positive control) 100 Fern Extract (0.01%) 23 Lotus Extract (0.01%) 20 Elm Extract (0.01%) 22 Fern, Lotus, Elm Extract (0.01%) 25

As can be seen in Table 1, the fern, lotus, elm extract in human normal fibroblasts promoted the production of type I procollagen compared to the negative control. Therefore, it can be seen that the fern, lotus, and elm extract of the present invention promote collagen production to alleviate the reduction of collagen caused by aging of the human skin, thereby having an excellent anti-aging effect.

Experimental Example  2. Progtaglandin E2 ( PGE2 Inhibitory effect experiment

In order to confirm the inhibitory effect of prostaglandin E2 (PGE2) production, the inflammatory factor Protetaglandin E2 (PGE2), human keratinocyte cell line HaCaT cells were cultured in a 24-well plate for 24 hours in a concentration of 5 × 10 ⁴ lipopolysac After inducing prostaglandin production with 5 μg / ml of a lip (lipopolysaccharide (LPS)), fern, lotus, and elm extracts were treated for 24 hours. 100 μl of the culture supernatant was taken to quantify the produced prostaglandins using the kit of the R & D system, and the results are shown in Table 1 below.

(1)

% Inhibition = (A-B) / A × 100

A: PGE2 generation amount in LPS treatment compared to negative control group

B: PGE2 generation amount in sample processing compared to negative control group

PGE2 production inhibition rate (%) Fern Extract (0.01%) 44 Lotus Extract (0.01%) 47 Elm Extract (0.01%) 46 Fern, Lotus, Elm Extract (0.01%) 52

When the fern, lotus, and elm extracts were treated, prostaglandin production was inhibited by 52%, and the anti-inflammatory effect was confirmed through inhibition of progtaglandin production of the fern, lotus and elm extracts.

Experimental Example  3. Safety test for skin

Human patch test was performed to evaluate the degree of irritation to the skin. The skin patch test was carried out on 20 normal people over 20 years old. The samples were prepared by 1% and 10% aqueous solution, and then attached to the inner region of the upper arm for 48 hours using a Finn chamber. After 72 hours, a second reading was performed to determine the skin reaction result. Evaluation of the evaluation criteria and the stimulation index is shown in Table 3, the results are shown in Table 4 below.

reaction weight Evaluation standard - 0.0 No response ± 0.5 Suspected positive reaction + 1.0 Weak positive reaction ++ 2.0 Strong positive reaction +++ 3.0 Polar positive reaction

(2)

Skin irritation index = Σ (weighted number of responders) / (highest weight X total number of subjects) × 100

Skin irritation index Stimulation degree Fern Extract (1%) 0.4 No stimulation Lotus Extract (1%) 0.4 No stimulation Elm Extract (1%) 0.4 No stimulation Fern, Lotus, Elm Extract (1%) 0.4 No stimulation Fern, Lotus, Elm Extract (10%) 0.8 No stimulation

As a result of the experiment, fern, lotus and elm extract were found to be non-irritating to the skin at less than 10%.

Experimental Example  4. Moisturizing  Measurement test

The cream compositions of Examples 1 to 4 and Comparative Example 1 were prepared in the following wt.

Ingredients Content (% by weight) Comparative Example 1 Example One 2 3 4 Fern extract - 1.0 - - 0.33 Lotus extract - - 1.0 - 0.33 Elm extract - - - 1.0 0.33 glycerin 5.0 5.0 5.0 5.0 5.0 Stearic acid 8.0 8.0 8.0 8.0 8.0 Squalane 5.0 5.0 5.0 5.0 5.0 Self-emulsifying glycerin monostearate 2.5 2.5 2.5 2.5 2.5 Monostearic acid polyoxyethylene sorbitan 1.5 1.5 1.5 1.5 1.5 Propylene glycol 4.0 4.0 4.0 4.0 4.0 Stearyl glycyrrhetinate 0.2 0.2 0.2 0.2 0.2 vaseline 2.0 2.0 2.0 2.0 2.0 Antioxidant Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance

Moisturizing power measurement test was carried out in a constant temperature and humidity room with a temperature of 22 ° C. and a relative humidity of 45%. On the inside of the forearm of 20 test subjects, a square width × length 1.5 cm × 1.5 cm is indicated by the number of samples. The moisture content of the skin was measured before applying a certain amount of the creams prepared in Examples and Comparative Examples 1 to 4, after 1 hour and after 2 hours of application, and normal skin without any treatment was used as a control example. It was. The measuring device used a moisture content measuring instrument (Corneometer CM825, Courage + Khazaka, Germany) to measure the moisture content by measuring the electrical capacity of the skin according to the moisture content of the skin. The moisturizing improvement rate is shown in Table 6 below.

(3)

Moisturizing Improvement (%) = (B-A) / A × 100

A: Moisture measurement value before application

B: Moisture measurement value after sample application

Moisturizing improvement after 1 hour (%) Moisturizing improvement after 2 hours (%) Control Example (No Treatment Group) 8 9 Comparative Example 1 25 23 Example 1 36 30 Example 2 35 30 Example 3 38 32 Example 4 43 35

As a result of the experiment, when Examples 1 to 4 containing fern, lotus, and elm extract were applied to the skin, the effect of improving the moisturizing power was confirmed.

Experimental Example  5. Wrinkle improvement test on human skin

In order to confirm the skin wrinkle improvement effect of the cream prepared in Comparative Example 1 and Examples 1 to 4 was conducted a clinical trial.

For 25 women in their 20s and 40s, the creams prepared in Comparative Example 1 and Examples 1 to 4 were divided into 5 groups of 5 people, which were used twice a day after morning and evening face washes for 4 weeks.

Skin wrinkle improvement before and after use was measured using an anterior vision imaging system (facial stage DM-3 (Moritex, Japan)), and the effect of obtaining wrinkles area change after 4 weeks using an analysis program was compared. The results are shown in Table 7.

(Equation 4)

Wrinkle area change = wrinkle area before use-wrinkle area after 4 weeks

Wrinkle area variation (mm ² ) Comparative Example 1 2 Example 1 8 Example 2 7 Example 3 8 Example 4 10

As a result, when using Examples 1 to 4 containing fern, lotus, elm extract for 4 weeks, it was confirmed that the effect of improving the skin wrinkles compared to Comparative Example 1.

Hereinafter, based on the results of the experimental example described above, various formulation examples using the fern, lotus, elm extract are presented. However, these formulation examples are intended to illustrate the present invention, and it is apparent to those skilled in the art that the formulation of the present invention is not limited to these formulation examples.

Formulation Example  1.Flexible Lotion ( skin toner )

The components and contents of Formulation Example 1 are shown in Table 8.

Ingredients Content (% by weight) Fern extract 0.33 Lotus extract 0.33 Elm extract 0.33 glycerin 5.0 1,3-butylene glycol 3.0 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.5 Spices Suitable amount antiseptic Suitable amount Purified water Balance

Formulation Example  2. Converging lotion ( astrigent )

The components and contents of Formulation Example 2 are shown in Table 9.

Ingredients Content (% by weight) Fern extract 0.33 Lotus extract 0.33 Elm extract 0.33 glycerin 3.0 Citric acid 0.1 ethanol 10.0 Polyoxyethylene oleyl ether 1.0 Sorbitol 2.0 Spices Suitable amount antiseptic Suitable amount Purified water Balance

Formulation Example  3. Lotion ( emulsion )

The components and contents of Formulation Example 3 are shown in Table 10.

Ingredients Content (% by weight) Fern extract 0.33 Lotus extract 0.33 Elm extract 0.33 glycerin 5.0 1,3-butylene glycol 8.0 Squalane 10.0 Monooleic acid polyoxyethylene sorbitan 2.0 Triethanolamine 1.5 Glyceryl stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxyvinyl polymer 0.1 Arginine 0.1 Spices Suitable amount antiseptic Suitable amount Purified water Balance

While the present invention has been described with reference to exemplary embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. You will know. In addition, many modifications may be made to adapt a particular situation and material to the teachings of the invention without departing from the essential scope thereof. Accordingly, it is intended that the invention not be limited to the particular embodiment disclosed as the best mode contemplated for carrying out this invention, but that the invention be construed as including all embodiments falling within the scope of the appended claims.

Claims (9)

Cosmetic composition for skin regeneration containing at least one plant extract selected from the group consisting of ferns, lotuses and elm. The method of claim 1,
The cosmetic composition for skin regeneration, characterized in that the high ratio is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The method of claim 1,
The lotus flower is a cosmetic composition for skin regeneration, characterized in that contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The method of claim 1,
Cosmetic composition for skin regeneration characterized in that the elm is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The method of claim 1,
The cosmetic composition for skin regeneration, characterized in that the fern, lotus or elm promote the production of collagen. The method of claim 1,
The cosmetic composition for skin regeneration, characterized in that the fern, lotus or elm to supply moisture to the skin. The method of claim 1,
Skin, characterized in that the cosmetic composition is one or more formulations selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, shampoo, oil-in-water (O / W) type and water-in-oil (W / O) type. Cosmetic composition for regeneration. The method of claim 1,
At least one plant extract selected from the group consisting of ferns, lotuses and elm is a cosmetic composition for skin regeneration, characterized in that extracted with water, an organic solvent or a mixture thereof. The method of claim 8,
The organic solvent is a cosmetic composition for skin regeneration, characterized in that at least one selected from the group consisting of anhydrous or containing methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.