KR20100048592A - Cosmetic composition containing gibberellins and herbal extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing gibberellins and herb extract is provided to promote type 1 procollagen biosynthesis, to suppress tyrosinase activation, and to ensure anti-aging and moisturization. CONSTITUTION: A cosmetic composition contains gibberellins, gibberellins-like substance, and plant extract containing gibberellins or gibberellins-like substance; and herb extract such as Cnidii Rhizoma extracts, Angelica gigas Nakai, or artemisia extract. The gibberellin-like substance is GA 1(gibberellin A1), GA3, GA4, GA5, GA6, GA15, GA17, gibberellic acid, gibberellin methyl ester, diterpenes.

Description

Cosmetic composition containing gibberellins and herbal extracts

The present invention (a) one or more selected from the group consisting of gibberellin, gibberellin analogues, and plant extracts containing gibberellin or gibberellin analogs; It relates to a cosmetic composition containing (b) one or more herbal extracts selected from the group consisting of cheongung extract, Angelica extract and mugwort extract.

The cosmetic composition according to the present invention not only has an excellent effect of promoting the growth of keratinocytes and an active oxygen scavenging effect generated by ultraviolet rays, but also reduces the biosynthesis of MMP-1 by ultraviolet rays related to skin matrix degradation, thereby reducing the type 1 procollagen. It promotes biosynthesis, inhibits tyrosinase activity, and shows excellent skin aging and moisturizing effect.

The skin is divided into three layers of epidermis, dermis, and subcutaneous fat tissue in order from the outside, and it is the body's primary protective layer that protects body organs from temperature and humidity changes and external environmental stimuli such as ultraviolet rays and pollutants. It plays an important role in maintaining homeostasis such as regulation. However, ultraviolet rays, excessive physical, chemical irritation, stress, and malnutrition from the outside degrade the skin's normal function and promote skin aging such as loss of elasticity, keratinization, and wrinkle formation. In particular, keratinocytes are made in sequence and the outermost layers of old keratinocytes sometimes deviate, so if the keratinization process that maintains a constant thickness of the epidermis is not smooth, abnormal keratinocytes are formed and the stratum corneum is thickened on the skin surface. The skin becomes rough and dull, fine wrinkles are generated and eventually aging of the skin. In addition, the reduction of biosynthesis of type 1 procollagen through the biosynthesis of MMP-1 by UV rays related to the decomposition of free radicals and skin matrix produced by UV rays, and the decrease of fibroblast division in the dermal layer of skin are also associated with skin aging. There is this.

A hormone involved in the growth and germination of plant gibberellin, Ascomycetes jibe Pasteurella Fu chikuwa Roy belonging to (Gibberella fujikuroi), SPA Kell Roman Mani hoti coke (Sphaceloma manihoticola) metabolite gibberellin compound group and commercial gibberellin synthesis are possible, such as To date , there are about 110 species of plants identified by vascular and physiological gates, as well as by the asymptomatic fungi Giberella fuzikuroi and Sparkello roma manihotica . Gibberellin is a small-molecular compound. Gibberellin's work began in 1809 by observing the disease in rice paddies. The disease is caused by the fungus Gibberella fujikuroi, whose origin was confirmed in Japan in 1925. Plant growth, seed germination, stem elongation, leaf growth, flowering, and maturation of pulp It is known to regulate. Commercially available production of the 110 kinds of gibberellin analogues are A ₃ type and analogs of different origins have the same effect as gibberellin in plants. In addition, these days, it is known that by treating the extract of a specific seed containing gibberellin or gibberellin analogues in a natural way instead of synthetic gibberellin, it is known that germination effects equivalent to or higher than those treated with gibberellin can be obtained.

Gibberellin is the first hormone in plants to allow seeds to start germinating. Seeds of plant herds belonging to the Pizza Botanical Gate, which have organs of endosperm or cotyledon, have a number of mechanisms that keep them dormant to prevent germination and to adapt to favorable circumstances. In this dormant state, all activities such as plant development and cell division are suspended, but they have a potential to be revived at any time.

Gibberellin is a hormone that breaks this dormant state and starts germination. When the embryos in the seed are suitable for germination, such as humidity and temperature, gerbere are secreted to convert many proteins and substances necessary for germination to convert proteins present in the whistle layer into amino acids. The enzymes and materials synthesized in this way are transferred to the udder to supply the necessary material for the embryonic cells to actively grow and divide.

Accordingly, the present inventors have tried to find a raw material having a skin moisturizing and anti-aging effect without causing skin side effects among natural substances, and (a) as a plant extract containing gibberellin, gibberellin analogues, and gibberellin or gibberellin analogues. One or more selected from the group consisting of; And (b) one or more herbal extracts selected from the group consisting of astronomical extracts, Angelica extracts and mugwort extracts; the cosmetic composition comprising as an active ingredient the growth promoting effect of keratinocytes, the active oxygen scavenging effect, the biosynthesis of MMP-1 The present invention was found to be excellent in promoting the biosynthesis of Type 1 procollagen, anti-aging effect, melanin production inhibitory effect and skin moisturizing effect.

Accordingly, an object of the present invention is to provide a cosmetic composition excellent in skin moisturizing, elasticity improvement and anti-aging effect.

In order to achieve the above object, the present invention (a) at least one selected from the group consisting of gibberellin, gibberellin analogues, and plant extracts containing gibberellin or gibberellin analogs; It provides a cosmetic composition comprising; and (b) at least one herbal extract selected from the group consisting of cheongung extract, Angelica extract and mugwort extract.

The cosmetic composition of the present invention is (a) at least one member selected from the group consisting of gibberellins, gibberellin analogues, and plant extracts containing gibberellin or gibberellin analogs; And (b) one or more herbal extracts selected from the group consisting of celestial extracts, Angelica extracts and mugwort extracts; as an active ingredient, promoting the growth of keratinocytes, the active oxygen scavenging effect produced by UV light, and the degradation of skin substrates. It is excellent in the effect of reducing the biosynthesis of MMP-1 due to the ultraviolet rays related to promoting the biosynthesis of type 1 procollagen, the inhibitory effect of tyrosinase activity, the anti-aging effect and the skin moisturizing effect.

Therefore, the composition of the present invention is a composition for promoting keratinocyte growth, a composition for promoting skin antioxidant, reducing the biosynthesis of MMP-1, a composition for promoting type 1 procollagen biosynthesis, a composition for promoting skin whitening, a composition for preventing skin aging, and skin It can be usefully used in cosmetics as a moisturizing enhancing composition.

The present invention (a) one or more selected from the group consisting of gibberellin, gibberellin analogues, and plant extracts containing gibberellin or gibberellin analogs; It provides a cosmetic composition comprising; and (b) at least one herbal extract selected from the group consisting of cheongung extract, Angelica extract and mugwort extract.

Hereinafter, the present invention will be described in more detail.

Gibberellin, which is used as an active ingredient in the composition of the present invention, is a hormone related to plant growth and germination, which is involved in the growth of stems, the promotion of fruit development, and the shedding of hibernation, and initiates germination of seeds. Is a hormone. When the embryos in the seed are suitable for germination, such as humidity and temperature, gerberis are secreted to synthesize numerous enzymes and substances necessary for the conversion of proteins in the whistle layer to amino acids and the nutrients necessary for germination. These synthesized enzymes and substances migrate to the milk and supply the necessary materials for the embryonic cells to actively grow and divide. The gibberellin can be separated by a conventional separation method, the method is not particularly limited.

Gibberellin analogs used as active ingredients in the compositions of the present invention refers to materials and precursors having the same effect as gibberellin, such as plant length growth, germination, etc. in the treatment of plants. Gibberellin analogues of the present invention include gibberellin compound family isolated from the plant corresponding to the vascular plant and portal plant, gibberellin compound family and metabolite of the asymptomatic fungi, and commercially available gibberellic acid. Specific examples of gibberellin analogs that can be used in the present invention include GA ₁ (gibberellin A ₁ ), GA ₃ , GA ₄ , GA ₅ , GA ₆ , GA ₁₅ , GA ₁₇ , gibberellic acid, gibberelline methylester, anseriogel and diterpene And the like.

Plants corresponding to the phyto-physical and portal plants are rice (Oryza Sativa L.), cherry (Prunus cerasus L.), citrus fruits (Citrus Fruits), Soneratia apetala (Sonneratia apetala), cucumber (Cucumis) sativus, Red beans (Phaseolus Multiflorus), Vine fern (Lygodium microphyllum), Lygodium reticulatum and Physcomitrella patens .

The fungus includes Gibberella fujikuroi, Sphaceloma manihoticola, Red fungus (Neurospora spp.), And Phaeosphaeria sp .

Representative compound structural formulas of gibberellins and gibberellin analogues according to the present invention are represented by the following Chemical Formula 1.

In addition, the plant extract containing the gibberellin or gibberellin analogues refers to a plant extract containing gibberellin or gibberellin analogues isolated from the seed itself or germinated seeds germinated from the seed naturally or in a conventional germination method as an active ingredient. Plant extracts may be prepared according to conventional methods known in the art, and the method is not particularly limited.

Specific examples of plant extracts comprising gibberellin or gibberellin analogues usable in the present invention include botanical (peach seed) extracts, rice extracts, barley extracts, cucumber seed extracts, corn extracts, and the like.

At least one selected from the group consisting of gibberellins, gibberellins analogues, and plant extracts containing gibberellins or gibberellins analogs of the present invention is preferably contained in 0.0001 to 25.0% by weight relative to the total weight of the composition. If the content is less than 10,000% by weight, the effect is insignificant, and if the content exceeds 25.0% by weight, there is a problem of the feeling of use of the product and the stability of the formulation.

Cnidium officinale, which is used as an active ingredient in the composition of the present invention, is a perennial herbaceous herbaceous plant with a height of 40-70 cm and has a scent, and serves for wound healing, skin regeneration, and antibacterial activity. In addition, it is excellent in the melanocyte stimulating hormone inhibitory effect excellent whitening effect.

Angelica Gigantis Radix, which is used as an active ingredient in the composition of the present invention, has a peculiar smell and tastes slightly sweet as the root of Angelica gigas NAKAI, a plant of the Umbeliferae plant. Angelica is excellent in the blood-producing action to produce blood and the blood activity to circulate blood smoothly, anti-cancer effect and blood pressure lowering effect is strong. Anti-aging and regulating peristalsis of the intestine remove chronic constipation and have excellent skin soothing, anti-inflammatory and whitening effects.

In addition, mugwort (Artemisia asiatica) used as an active ingredient in the composition of the present invention is a perennial herb of the compositae, hemostatic, analgesic, astringent, branch, anti-inflammatory, diuretic and antipyretic. It also has antioxidant, anti-allergic and anti-inflammatory effects and promotes skin regeneration and elasticity to maintain skin health.

Extracts of the cheonggung, tangui and wormwood may be prepared according to conventional methods known in the art, the method is not particularly limited. In general, after pulverizing the cheongung, Angelica and mugwort, respectively, the extraction solvent is added and heated to extract the active ingredient and concentrated under reduced pressure to obtain an extract. As the extraction solvent, a solvent that can be generally used in cosmetics may be used, and examples thereof include water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol.

One or more herbal extracts selected from the group consisting of cheon-ung extract, Angelica extract and mugwort extract of the present invention is preferably contained in 0.0001 ~ 25.0% by weight relative to the total weight of the composition. If the content is less than 0.0001% by weight, the effect is insignificant, and when the content is more than 25.0% by weight, the usability of the product and stability of the formulation occur.

On the other hand, the celery extract, Angelica extract and mugwort extract of the present invention can be used separately or in a mixture. In the mixed herbal extracts, the mixing ratio of Cheongung extract, Angelica extract and mugwort extract is preferably 1: 1: 1 to 10: 1: 1 in consideration of its effect and application as a cosmetic.

The cosmetic composition according to the present invention has excellent growth promoting effect of keratinocytes, active oxygen scavenging effect generated by ultraviolet light, type 1 procollagen biosynthesis promoting effect through reduction of biosynthesis of MMP-1 by ultraviolet light related to skin matrix degradation, and It is excellent in inhibiting tyrosinase activity and shows excellent skin anti-aging and moisturizing effect. Therefore, the composition of the present invention is a composition for promoting keratinocyte growth, a composition for promoting skin antioxidant, reducing the biosynthesis of MMP-1, a composition for promoting type 1 procollagen biosynthesis, a composition for promoting skin whitening, a composition for preventing skin aging, and skin It can be usefully used in cosmetics as a moisturizing enhancing composition.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other components that can give a synergistic effect to the main effect, preferably within the range of not impairing the main effect for which the present invention is intended. In addition to the active ingredient, other ingredients can be appropriately selected and blended by those skilled in the art according to the formulation or use purpose of other cosmetics.

For example, the cosmetic composition may contain a skin absorption promoting substance to increase the effect. In addition, the cosmetic composition of the present invention may include a material selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. In addition, the cosmetic composition of the present invention may include other components which are usually formulated into cosmetics as necessary in addition to the essential components, and examples thereof include fats and oils, humectants, emollients, surfactants, organic and inorganic substances. Pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, limiting agents and purified water.

Other compounding components that can be included in the cosmetic composition of the present invention is not limited thereto, and the compounding amount of the above components is possible within a range that does not impair the object and effect of the present invention.

The cosmetic composition according to the present invention is not particularly limited in the formulation, and for example, emulsion, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, Formulations such as cleansing foams, cleansing creams, cleansing water, soaps or sprays and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

[Test Example 1] keratinocyte growth promoting effect (MTT assay) effect

The effect of the herbal extracts mixed with Gibberellin A ₃ , Gibberellic acid, and causal extracts, and medicinal herb extracts, Angelica extracts, and mugwort extracts on the growth ability of keratinocytes was measured by MTT method.

Gibberellin A ₃ and gibberellic acid were purchased from Sigma Aldrich, and phosphorus extract was purchased from Bioland, South Korea. Cheonggung extract, Angelica extract and mugwort extract were purchased from Bioland Co., Ltd. (Bioland, South Korea) and each extract was mixed at a ratio of 1: 1: 1 and used as a mixture of herbal extracts.

First, the keratinocytes were planted in a 96 well plate at a concentration of 5 * 10 ³ per 200 μl per well and incubated for 24 hours, and then gibberellin A ₃ , gibberellic acid or phosphorus extracts were 0.001, 0.01 and 0.1, respectively. %, And the mixture of herbal extracts were treated at 10% concentrations for 48 hours, incubated for 48 hours, and then aspirated and removed, washed once with PBS and MTT (Methylthiazolyldiphenyl-tetrazolium bromide, Sigma, USA) (0.5 Mg / ml) solution was added to the cells at 100 μl and incubated at 37 ° C., 5% CO ₂ for 4 hours. Thereafter, the culture solution was aspirated and removed, and then 200 µl of DMSO (dimethyl sulfoxide) solution was added, shaken for 10 minutes in a shaker, and the absorbance at 540 nm was read with an ELISA reader (DIbiotech, Korea). . At this time, as a control group, gibberellin A ₃ , gibberellic acid, and the mixed solution untreated group of medicinal extract and herbal extracts were used, the results are shown in Table 1 below.

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.001% 135 192 0.01% 136 243 0.1% 143 276 Gibberellic acid 0.001% 125 175 0.01% 126 198 0.1% 138 227 Doin (Peach Seed) Extract 0.001% 148 188 0.01% 152 224 0.1% 162 250 Untreated control 0% 100 121

As shown in Table 1, the gibberellin A ₃ , gibberellic acid and phosphine extract used as an active ingredient in the present invention was all excellent in promoting the growth of keratinocytes. In addition, the use of herbal extract mixture with Gibberellin A ₃ , Gibberellic acid, or Doin extract increased the growth of keratinocytes faster, and the effect of promoting the division of keratinocytes increased proportionally with the concentration of each component. .

Test Example 2 Active Oxygen Scavenging Effect

In order to investigate the inhibitory effect of free radical production produced by UV light of the herbal extracts of Gibberellin A ₃ , Gibberellic acid or Doin extract, Cheongung extract, Angelica extract and mugwort extract, The experiment was performed as follows.

First, the cell line used in the experiment was a human keratinocytes HaCaT cell line distributed from Dr. Fusenig of the German Cancer Research Center, which is a 96 well black plate for fluorescence measurement. Dispense at 2.0 * 10 ⁴ per well, and at 37 ° C., 5% CO ₂ conditions using DMEM (Dulbeccos Modification of Eagles Medium, FBS 10%, Gibco, USA) medium supplemented with penicillin / streptomycin After incubating for 1 day at 10%, the mixture of 10% herbal extract and gibberellin A ₃ , gibberellic acid or phosphorus extract was 0.5, 0.25, 0.125, 0.0625 and 0.03125%, respectively. The concentration was treated.

Next, the test sample was added and incubated for 24 hours, and then washed with HCSS (HEPES-buffered control salt solution, Gibco, USA) to remove the remaining medium, and DCFH-DA (2 ', 7'-dichlorodihydro prepared in 20 μM in HCSS). 100 μl of fluorescein diacetate, Molecular Probes, USA) was incubated at 37 ° C. and 5% CO ₂ for 20 minutes and washed with HCSS. After 100 μl of HCSS treated for each sample concentration, the fluorescence of DCF (dichlorofluorescein) oxidized with active oxygen was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). After UVB (30mJ / cm ² ) was irradiated and measured immediately after the treatment (Table 2) and 3 hours after the treatment (Table 3) by the fluorescent plate reader (Ex = 485nm, Em = 530nm). At this time, as a control group, an untreated group of gibberellin A ₃ , a gibberellic acid and a phosphorus extract untreated group and a herbal extract mixture was used.

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.03125% 84.82 77.26 0.0625% 84.02 76.93 0.125% 82.11 74.22 0.25% 80.10 71.43 0.5% 79.95 70.84 Gibberellic acid 0.03125% 87.56 77.84 0.0625% 85.84 77.00 0.125% 84.09 74.93 0.25% 82.88 72.93 0.5% 80.04 72.32 Doin (Peach Seed) Extract 0.03125% 88.03 80.82 0.0625% 86.37 79.87 0.125% 84.09 78.01 0.25% 82.46 76.93 0.5% 81.00 75.56 Untreated control 0% 100 98

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.03125% 79.93 70.83 0.0625% 77.29 65.96 0.125% 75.98 62.02 0.25% 72.79 60.21 0.5% 69.01 59.43 Gibberellic acid 0.03125% 81.11 71.19 0.0625% 79.43 69.01 0.125% 77.84 66.78 0.25% 73.07 62.59 0.5% 71.91 60.22 Doin (Peach Seed) Extract 0.03125% 80.54 70.22 0.0625% 76.09 68.75 0.125% 73.35 65.97 0.25% 71.69 63.22 0.5% 70.12 61.23 Untreated control 0% 100 92

As shown in Tables 2 to 3, Gibberellin A ₃ , Gibberellic acid and Doin extract used as the active ingredient in the present invention was all found to have an excellent effect of inhibiting the production of active oxygen. In addition, the use of herbal extract mixture with Gibberellin A ₃ , Gibberellic acid or Doin extract further increased the effect of inhibiting the production of free radicals, and as the concentration of each component increased, the effect of inhibiting the production of free radicals also increased proportionally. .

Test Example 3 Free Radical Scavenging Effect

In order to determine the free radical scavenging effect of the herbal extracts of Gibberellin A ₃ , Gibberellic acid or phosphine extract and cheongung extract, Angelica extract and mugwort extract were tested by the following method.

As a measuring method, 1,1-diphenyl-2-pyrylylhydrazyl (1,1-Diphenyl-2-pycryl-Hydrazyl; DPPH) method was used, and 1,1-diphenyl-2-picryl The 2-hydrazyl reagent was purchased from Sigma, USA (St. Louis, MO, USA) and used. This reagent is a relatively stable free radical and is the first in vitro method used to confirm the free radical scavenging action.

The concentration of 5.0, 1.0, 0.1, and 0.01% of the Gibberellin A ₃ , Gibberellic acid or Doin extract used for DPPH measurement was added to a 96 well plate at a concentration of 10%, and the DPPH prepared as a 5 mM ethanol solution. After the total volume was 200µl and left at 37 ° C for 30 minutes, the absorbance was observed with a 520nm ELISA reader. The free radical scavenging action (%) was calculated by the following Equation 1, and the results are shown in Table 4 below.

Free radical scavenging effect (%) = 100-(B / A) 100

A: Absorbance of control wells without sample

B: Absorbance of Experimental Wells Treated with Samples

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.01% 21.9 33.1 0.1% 36.7 54.2 One% 55.2 85.6 5% 83.0 97.2 Gibberellic acid 0.01% 10.8 29.8 0.1% 26.6 42.9 One% 54.2 75.2 5% 73.0 91.2 Doin (Peach Seed) Extract 0.01% 15.3 26.6 0.1% 31.9 50.6 One% 59.3 79.1 5% 76.8 95.0 Untreated control 0% 0 8.1

As shown in the results of Table 4, it was found that the Gibberellin A ₃ , Gibberellic acid, and Doin extract, which are used as an active ingredient in the present invention, are excellent in eliminating free radicals. In addition, the use of herbal extract mixture with gibberellin A ₃ , gibberellic acid or phosphine extract also increased the effect of free radical scavenging, and the free radical scavenging effect increased proportionally with the concentration of each component.

Test Example 4 Increased Antioxidant Protective Activity of Human Cell Lines

Cell lines used in this experiment were divided into 6.0 * 10 ⁶ cells in 150 * 150 culture plate with Human keratinocytes HaCaT cell line, and cultured for 1 day, and then, Gibberellin A ₃ , Gibberellic acid and Doin extracts were 5.0%, 1.0% and 0.1% concentrations. The mixed solution of the crude herbal extract was treated to the test sample at 10% concentration. DMEM (Dulbeco's modified eagles medium, GIBCO BRL, Life Technologues) medium containing 10% fetal bovine serum (FBS) used for the culture was used and cultured at 37 ℃, 5% CO ₂ conditions. After incubation, 1 * PBS buffered saline (Sigma Co.) was used for washing. After the test sample was added and cultured for 1 day, UVB was treated at 30mJ / cm ² intensity, cultured for 1 day at 37 ° C. and 5% CO ₂ , and then harvested and used to measure antioxidant defense factors. Antioxidant defense factors to be measured are superoxide dismutase (SOD), catalase and glutathione (GSH). Superoxide dismutase was measured by McCord's method (J. Biol. Chem). 1969; 244: 6049-6055), catalase was measured according to Budhuin's method (Biochem. J. 1964; 92: 179-184), and glutathione according to Akerboom's method (Methods in Enzymology 1981; 77: 373-382). .

The antioxidant protective factor recovery effect was expressed in% of the activity compared to this value in terms of 100% of the control (Control) not irradiated with UVB, the results are shown in Table 5 below.

density% Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% SOD Catalase GSH SOD Catalase GSH Control 100 100 100 100 100 100 UVB treatment group 45.5 67.2 62.5 49.2 70.3 71.1 Gibberellin A ₃ 0.1% 50.9 65.2 63.1 77.0 80.1 76.9 One% 68.3 72.1 79.2 90.6 91.2 89.3 5% 78.9 79.8 90.1 96.2 96.1 95.8 Gibberellic acid 0.1% 42.3 64.9 60.2 50.4 70.6 65.7 One% 65.4 71.1 73.1 77.0 76.4 79.2 5% 66.1 77.6 81.2 80.0 80.0 92.1 Doin (Peach Seed) Extract 0.1% 45.6 67.8 61.9 53.0 69.9 72.2 One% 69.4 71.1 78.8 71.5 77.6 85.4 5% 72.6 77.3 88.6 83.2 79.5 94.0

As shown in the results of Table 5, Gibberellin A ₃ , Gibberellic acid and doin extract used as an active ingredient in the present invention all can be seen that the antioxidant efficacy is excellent by restoring the activity of antioxidant defenses reduced by ultraviolet light there was. In addition, the use of herbal extract mixture with gibberellin A ₃ , gibberellic acid or phosphine extract also increased the effect of restoring the activity of antioxidant defenses, and as the concentration of each component increased the antioxidant effect proportionally increased.

Test Example 5 MMP-1 and Procollagen Assay

MMP-1 and Procollagen Analysis to Determine the Biosynthesis Reduction of MMP-1 and Promoting Biosynthesis of Type 1 Procollagen by Gibberellin A ₃ , Gibberellic Acid and Doin Extracts, Herbal Extracts, Angelica Extracts, and Mugwort Extracts (procollagen assay) was performed in the following manner.

First, the fat layer of the normal epidermis is removed and chopped to separate the epidermis and the dermis with collagenase, and then the epidermis and the dermis are put into 0.25% trypsin solution, respectively, at 37 ° C. and 5% CO ₂ incubator. Treated for a minute. Since vortex was performed to release the keratinocytes and fibroblasts, respectively. After collecting and washing the free cells, keratinocytes were cultured at a concentration of 1 * 10 ⁴ cells / cm ² in KGM (keratinocyte growth medium, Clonetics, USA), and fibroblasts were added with 10% fetal bovine serum (FBS). Cultured in DMEM medium. When grown at about 70-80%, the cells were subcultured at a ratio of 1: 3, and cells subjected to 3-4 subcultures were used for the experiment.

For experiments to measure the amount of MMP-1 fibroblasts were cultured in more than 90% in a 48 well plate (star plate) was placed in a starvation (starvation) state for one day. After washing twice with PBS, the lid of the plate (plate) was opened with 100 μl of PBS and irradiated with UV A (Dermlight cube 401, UVAtec, USA) equipped with UVA filters at 15 J / cm ² . Washed once again with PBS Immediately after irradiation, and the gibberellin A _3, gibberellin acid, degree extract each 0.001, 0.01%, and put in a DMEM which does not include fetal calf serum containing a mixture of 10% of the herbal extract 37 ℃, 5% CO After 48 hours of incubation in ₂ incubators, the amount of free MMP-1 in the medium was measured using the MMP-1 human ELISA system (RPN2610, Amersham Pharmacia Biotech, UK) and the total number of fibroblasts Corrected by protein amount (Table 6). At this time, as a control group, a mixed solution untreated group of gibberellin A ₃ , gibberellic acid, and a causal extract untreated group and a herbal extract was used.

Meanwhile, in order to measure the amount of procollagen, gibberellin A ₃ , gibberellic acid, and phosphine extracts 0.001, 0.01% and herbal extracts, respectively, without UV irradiation under starvation under the same conditions as the method for measuring the amount of procollagen. 10% of the mixture was added, and after 24 hours, the amount of free collagen in the medium was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan). It was measured (Table 7). At this time, as a control group, a mixed solution untreated group of gibberellin A ₃ , gibberellic acid, and a causal extract untreated group and a herbal extract was used.

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.001% 70 65 0.01% 66 51 Gibberellic acid 0.001% 77 74 0.01% 72 61 Doin (Peach Seed) Extract 0.001% 79 70 0.01% 66 54 Untreated control 0% 100 99

density(%) Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 0% 10% Gibberellin A ₃ 0.001% 107 135 0.01% 115 144 Gibberellic acid 0.001% 117 123 0.01% 125 135 Doin (Peach Seed) Extract 0.001% 109 127 0.01% 125 137 Untreated control 0% 100 103

As shown in the results of Table 6, it was found that the gibberellin A ₃ , gibberellic acid and the extract of causal used as an active ingredient in the present invention all inhibit MMP-1. In addition, the use of herbal extract mixture with gibberellin A ₃ , gibberellic acid or phosphine extract further increased the inhibitory effect of MMP-1, and as the concentration of each component increased, the amount of MMP-1 decreased proportionally.

As shown in the results of Table 7, it was found that the gibberellin A ₃ , gibberellic acid and doin extract used as active ingredients in the present invention all increase the biosynthesis of type 1 procollagen. In addition, the use of herbal extract mixture with Gibberellin A ₃ , Gibberellic acid or Doin extract further increased the biosynthetic effect of Type 1 procollagen, and as the concentration of each component increased, the amount of procollagen produced increased proportionally. It was.

EXAMPLES 1-6 AND COMPARATIVE EXAMPLES 1-2

The cosmetic compositions of Examples 1 to 6 and Comparative Examples 1 and 2 were prepared according to the compositions of Table 8 below.

Ingredients (content: wt%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 1.Beads wax 2 2 2 2 2 2 2 2 2. Stearyl Alcohol 3 3 3 3 3 3 3 3 3. Stearic acid 8 8 8 8 8 8 8 8 4. Squalene 10 10 10 10 10 10 10 10 5. Propylene Glycol Monostearate 3 3 3 3 3 3 3 3 6. Polyoxyethylene ether One One One One One One One One 7. Preservative Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 8. Glycerin 5 5 5 5 5 5 5 5 9. Propylene Glycol 4 4 4 4 4 4 4 4 10. Triethylamine One One One One One One One One 11. Sodium Polyacrylate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 12. Purified Water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 13. Gibberellin A ₃ 5 - - - - - - - 14. Gibberellic acid - 5 - - - - - - 15. Doin Extract - - 5 5 5 5 - - 16. Cheongung Extract - - - 5 - - - - 17. Angelica Extract - - - - 5 - - - 18. Mugwort Extract - - - - - 5 - - 16. Herbal Extracts Containing Cheonung Extract, Angelica Extract and Mugwort Extract 10 10 10 - - - - 10 17. Carbomer Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity

<Production method>

1) The components 1-7 were melt | dissolved by heating at 65-75 degreeC, and the oil phase part was manufactured.

2) Components 8-12 were dissolved by heating at 65-75 ° C., thereby preparing an aqueous part.

3) While stirring 2), 1) and component 17 were slowly added and emulsified at 7000 to 8000 rpm for 5 minutes to prepare a cream formulation.

4) After cooling to 45 ℃ was added to components 13-16 and dispersed for 1 minute at 1500rpm.

[Test Example 7] Wrinkle improvement effect

In order to examine the wrinkle improvement effects of Examples 1 and Comparative Examples 1 and 2, 140 women in their 30s were divided into 7 groups of 20 people each, and Example 1 and Comparative Example 1 were applied to the eye area designated twice a day for 8 weeks each morning and evening. After applying the cream of ~ 2 each, a replica of silicon was made, and the condition of wrinkles at the designated area was measured by a visometer (SVI60, Courage + Khazaka electronic GmbH, Germany), an image analyzer. The results are shown in Table 9 below. This result represents the average of each parameter value after 8 weeks minus the parameter value 8 weeks ago. In other words, the negative the value, the higher the wrinkle improvement effect.

R1 R2 R3 R4 R5 Example 1 -0.23 -0.20 -0.12 -0.09 -0.08 Comparative Example 1 0.28 0.26 0.22 0.04 0.03 Comparative Example 2 -0.10 -0.09 -0.05 -0.05 -0.03

R1: difference between the highest and lowest of the wrinkle contour

R2: Average of R1 values after randomly dividing the wrinkle contour by 5 spaces

R3: Highest value of R1 divided by 5

R4: Baseline of the wrinkle contour, minus the top and valley of each angle

R5: Average of the baseline of the wrinkle contour, minus each wrinkle contour

As can be seen in Table 9, Example 1 containing a mixed solution of gibberellin A ₃ and herbal extracts compared to Comparative Examples 1 to 2 showed a large negative value in all items. Therefore, it was found that the skin wrinkle improvement effect of the cosmetic composition of the present invention is very excellent.

Test Example 8 Skin Barrier Function Restoration Effect

In order to measure the skin barrier function recovery effect of Examples 1 to 3, acetone was repeatedly applied to the skin of hairless mice to impair barrier function, and then epidermal moisture loss (TEWL) was measured. When measured by servomed (Sweden) evaporimeter EP1 and reaches 4.0 g / m 2 / h, the above Examples 1-3 and Comparative Examples 1-2 were applied to an area of 5 cm 2, and after 1, 2, 4 and 8 hours, the epidermis The extent to which barrier function is restored is assessed by measuring the amount of water loss and evaluating the extent of the decrease. At this time, a conventional lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The epidermal moisture loss in the initial damaged barrier state was set to 100% and the change over time was compared, and the results are shown in Table 10 below.

sample Epidermal moisture loss 1 hour later 2 hours later 4 hours later 8 hours later Example 1 56 47 35 26 Example 2 69 60 48 39 Example 3 60 55 40 30 Comparative Example 1 89 80 76 72 Comparative Example 2 77 69 62 59 Lipid mixture 64 44 37 25

As can be seen from the results in Table 10, Examples 1 to 3 containing a mixed solution of herbal extracts with Gibberellin A ₃ , Gibberellic acid or Doin extract are less epidermal moisture loss than Comparative Example 1 containing no active ingredient. In addition, it showed better skin barrier function recovery effect than the positive control lipid mixture.

Test Example 9 Moisturizing Effect Test in Human Body

Appealing dry or 60 adult men and women who are considered to be dry skin divided into 5 sets, each of Examples 1 to 3 and Comparative Examples 1 to 2 were provided and applied to the face twice a day for 4 weeks. Moisturizing power was compared by measuring TEWL at constant temperature and humidity conditions (24 ° C., 40% relative humidity) at the time of 2 weeks and 4 weeks after the start of coating and after application, and the results are shown in FIG. 1. In addition, the skin moisture content was measured with a corneometer, and the results are shown in FIG. 2.

<How to measure Trans Epidermal Water Loss (TEWL)>

Principle: The amount of moisture evaporated from the skin is calculated according to the area and time, and the amount of moisture evaporated is read and quantified by the temperature and moisture electronic sensors to measure the moisture of the skin.

1. Environmental conditions: The temperature and humidity are kept constant, leaving only the minimum number of people except the meter and the subject. (If possible, shut off the flow of air.)

2. Place the TEWL probe on the measurement site of the subject.

3. Do not apply excessive force to the measuring part. Place the probe lightly so that no marks remain on the skin and make an angle of 90 degrees with the ground.

4. Measure the value that the skin's TEWL value becomes constant over time. (1 ~ 3 minutes)

5. Hold the probe handle as far as possible to prevent the body temperature from being transferred.

6. Calculate the amount of water evaporated per hour per area (g / m ² / h).

As can be seen from the results of Figure 1, when applying the skin to Examples 1 to 3 containing a mixture of herbal extracts with Gibberellin A ₃ , Gibberellic acid or Doin extract, TEWL changes over time (moisture) Evaporation) decreased. Therefore, it turned out that the moisturizing effect of the cosmetic composition of this invention is excellent.

<How to measure the Koniometer>

Principle: Moisture is measured by measuring the amount of moisture in the skin's epidermis using a sensor and quantifying the amount of moisture.

1. Place the probe of the Koniometer on the area of the skin to be measured.

2. Press the probe on the skin and the sensor will quantify the skin's electrical conductivity and display it on the screen.

3. Repeat the measurement with different measuring area.

4. After measuring once, wipe the sensor with Kimwipe and measure again.

As can be seen from the results of Figure 2, Examples 1 to 3 containing a mixture of herbal extracts with Gibberellin A ₃ , Gibberellic acid or Doin extracts are compared to Comparative Examples 1 and 2 in the amount of moisture increase in the skin over time This was found to be large, thereby better moisturizing effect of Examples 1-3.

Test Example 10 Usability Evaluation

96 adult men and women who were called dry or thought to be dry skin were divided into 8 sets, and each group was provided with Examples 1 to 6 and Comparative Examples 1 and 2 to be applied to the face twice a day for 4 weeks.

After using for 4 weeks, the evaluation of the skin dryness improvement effect was conducted through a questionnaire about usability, and the results are shown in Table 11 below.

Applicator Respondents Very good Good usually Inadequate Example 1 5 6 One 0 Example 2 4 7 3 0 Example 3 3 6 3 0 Example 4 2 4 6 0 Example 5 2 4 5 0 Example 6 2 5 5 0 Comparative Example 1 0 2 8 2 Comparative Example 2 One 3 7 One

As can be seen from the results of Table 11, the people who applied the Examples 1 to 6 was found to feel that the skin dryness improvement effect and moisturizing effect is relatively superior to those who applied the Comparative Examples 1 to 2.

Hereinafter, the formulation examples of the composition will be described, but this is not intended to limit the invention but only to explain in detail.

[Formulation Example 1] Suspension emulsion lotion

To prepare a suspension emulsion lotion in the composition of Table 12.

Ingredient Content (% by weight) Methylparaben flavor Cetyl Octanoate Polyoxyethylene Cured Castor Oil Polysiloxane Tocopherol Ethanol Glycerin Propylene Glycol Carboxyvinyl Polymer Gibberellins Analogs Suitable Quantity 1.0 0.6 0.4 0.1 12.0 6.0 2.0 0.12 1.5 50 TO 100

Formulation Example 2 Essence

To prepare an essence in the composition of Table 13.

Ingredient Content (% by weight) Methylparaben scent Cetyl Octanoate Octyldodes-16 Cholese-24 / Cetes-24 Polysiloxane Tocopherol Ethanol Glycerin Xanthan Gum Propylene Glycol Carboxyvinyl Polymer Gibberelella Analogs Suitable Quantity 1.0 0.5 0.2 0.4 0.1 6.0 15.0 0.07 4.0 0.12 6.0 50 TO 100

Formulation Example 3 Nutrition Cream

To the nutrition cream was prepared in the composition of Table 14.

Ingredient Content (% by weight) Liquid paraffin cetostearyl alcohol beeswax polysiloxane lipophilic monostearic acid stearate stearic acid cetearyl glucoside monostearic acid polyoxyethylene sorbitan (20 EO) hydrogenated lecithin cetyloctanoate methyl paraben propylparaben fragrance sodium ethylene amine tetraacetate Mixed solution of carboxyvinyl polymer propylene glycol ginkgo leaf extract herbal extract 15.0 2.0 3.0 0.5 2.0 2.0 1.5 1.3 2.0 1.0 0.2 0.1 Quantity 0.02 5.0 0.10 2.0 6.0 10 TO 100

Formulation Example 4 Gel

To prepare a gel in the composition of Table 15.

Ingredient Content (% by weight) Preservatives Flavors Cetearylglucoside Cetyloctanoate Glycerin Jojoba Wax DL-Pantenol Ethanol Carboxyvinyl Polymer Propylene Glycol Gibberellin-like Herbal Medicine Extract Purified Water Proper quantity 1.5 1.0 5.0 3.0 1.0 7.0 0.6 3.0 3.0 50 TO 100

[Formulation Example 8] Pack

To prepare a pack in the composition of Table 16.

Ingredient Content (% by weight) Methyl paraben fragrance Cetyl octanoate polyoxyethylene hardened castor oil polysiloxane cellulose gum ethanol glycerin propylene glycol polyvinyl alcohol gibberellin analogs Suitable Quantity 1.0 0.6 0.2 0.2 6.0 5.0 3.0 15.0 3.0 20 TO 100

1 is a graph measuring the moisturizing power of the composition according to the present invention by a method of measuring transdermal moisture loss (TEWL).

Figure 2 is a graph measuring the moisture content of the skin by the composition according to the present invention with a conniometer.

Claims (14)

(a) at least one member selected from the group consisting of gibberellins, gibberellin analogs, and plant extracts containing gibberellin or gibberellin analogs; And (b) one or more herbal extracts selected from the group consisting of Cheongung Extract, Angelica Extract and Mugwort Extract; Cosmetic composition containing the as an active ingredient. According to claim 1, wherein the gibberellol analogue is selected from the group consisting of gibberellin compound family isolated from the plant corresponding to the vascular plant door and the selection plant, gibberellin compound family metabolites of asymptococci and commercially available gibberellic acid Cosmetic composition, characterized in that at least one. According to claim 2, wherein the plants corresponding to the phyto-physical and portal plants are rice (Oryza Sativa L.), cherry (Prunus cerasus L.), citrus fruits (Citrus Fruits), Soneratia apetara (Sonneratia) 1 selected from the group consisting of apetala , cucumber (Cucumis sativus), red beans (Phaseolus Multiflorus), vine fern (Lygodium microphyllum), Lygodium reticulatum and Physcomitrella patens Cosmetic composition characterized by the above-mentioned. The method of claim 2, wherein the ascomycetes is jibe Pasteurella Fu chikuwa Roy (Gibberella fujikuroi), SPA Kell Roman Mani hoti coke (Sphaceloma manihoticola), red bread mold (Neurospora spp.) And par O Spa Area species (Phaeosphaeria sp.) Cosmetic composition, characterized in that at least one selected from the group consisting of. The method according to claim 1, wherein the gibberellol analogs are GA ₁ (gibberellin A ₁ ), GA ₃ , GA ₄ , GA ₅ , GA ₆ , GA ₁₅ , GA ₁₇ , gibberellic acid, gibberellin methyl ester, anseriogel and diterpenes Cosmetic composition, characterized in that at least one selected from the group consisting of. The cosmetic composition according to claim 1, wherein the plant extract containing gibberellin or gibberellin analogues is at least one member selected from the group consisting of ginseng extract, rice extract, barley extract, cucumber seed extract and corn extract. The method according to claim 1, wherein (a) at least one member selected from the group consisting of gibberellins, gibberellin analogues, and plant extracts containing gibberellin or gibberellin analogs is contained in an amount of 0.0001 to 25.0% by weight relative to the total weight of the composition Cosmetic composition. The cosmetic composition according to claim 1, wherein the medicinal herb extract is mixed in the ratio of Cheongung extract, Angelica extract and mugwort extract in a ratio of 1: 1: 1 to 10: 1: 1. The cosmetic composition according to claim 1, wherein the herbal extract is contained in an amount of 0.0001 to 25.0% by weight based on the total weight of the composition. The cosmetic composition according to any one of claims 1 to 9, wherein the composition is for promoting skin keratinocyte growth. The cosmetic composition according to any one of claims 1 to 9, wherein the composition is for skin antioxidant enhancement. The cosmetic composition according to any one of claims 1 to 9, wherein the composition is for promoting skin whitening. The cosmetic composition according to any one of claims 1 to 9, wherein the composition is for preventing skin aging. The cosmetic composition according to any one of claims 1 to 9, wherein the composition is for promoting skin moisturization.

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