KR101857825B1 - 변성 없이 가용성 재조합 인터페론 단백질을 제조하는 방법 - Google Patents

변성 없이 가용성 재조합 인터페론 단백질을 제조하는 방법 Download PDF

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KR101857825B1
KR101857825B1 KR1020127025760A KR20127025760A KR101857825B1 KR 101857825 B1 KR101857825 B1 KR 101857825B1 KR 1020127025760 A KR1020127025760 A KR 1020127025760A KR 20127025760 A KR20127025760 A KR 20127025760A KR 101857825 B1 KR101857825 B1 KR 101857825B1
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interferon
protein
pseudomonas
recombinant
ifn
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KR20130018743A (ko
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제프리 알렌
핑-후아 펭
아난트 팻카르
케이트 엘. 해니
로렌스 츄
레이 레이 폭햄 셍찬탈랑시
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피페넥스 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020127025760A 2010-03-04 2011-03-02 변성 없이 가용성 재조합 인터페론 단백질을 제조하는 방법 Expired - Fee Related KR101857825B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31067110P 2010-03-04 2010-03-04
US61/310,671 2010-03-04
PCT/US2011/026921 WO2011109556A2 (en) 2010-03-04 2011-03-02 Method for producing soluble recombinant interferon protein without denaturing

Publications (2)

Publication Number Publication Date
KR20130018743A KR20130018743A (ko) 2013-02-25
KR101857825B1 true KR101857825B1 (ko) 2018-05-14

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KR1020127025760A Expired - Fee Related KR101857825B1 (ko) 2010-03-04 2011-03-02 변성 없이 가용성 재조합 인터페론 단백질을 제조하는 방법

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Country Link
US (2) US9187543B2 (https=)
EP (1) EP2542574B1 (https=)
JP (1) JP5840148B2 (https=)
KR (1) KR101857825B1 (https=)
CN (1) CN102906108B (https=)
AU (1) AU2011223627B2 (https=)
BR (1) BR112012022292A2 (https=)
CA (1) CA2791361C (https=)
CL (1) CL2012002378A1 (https=)
CO (1) CO6592086A2 (https=)
ES (1) ES2639398T3 (https=)
MX (1) MX2012010148A (https=)
NZ (1) NZ602255A (https=)
PE (1) PE20130557A1 (https=)
RU (1) RU2573909C2 (https=)
WO (1) WO2011109556A2 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011109556A2 (en) 2010-03-04 2011-09-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
CN104151420A (zh) * 2013-05-15 2014-11-19 复旦大学 长效干扰素及其制备方法和用途
LT6164B (lt) 2013-10-15 2015-06-25 Uab Biotechnologinės Farmacijos Centras "Biotechpharma" Sulieti interferono-alfa 5 baltymai su kitu citokinu ir jų gamybos būdas
CN107099569B (zh) * 2017-06-30 2021-05-07 北京生物制品研究所有限责任公司 一种规模化发酵生产重组人干扰素β1b蛋白的方法
AU2021286574A1 (en) * 2020-06-09 2023-01-19 Icosavax, Inc. Method of making virus-like particle
CN116120424A (zh) * 2022-06-06 2023-05-16 江苏靶标生物医药研究所有限公司 一种干扰素α2b可溶性重组表达和分离纯化方法及其应用

Citations (1)

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US20050283000A1 (en) * 2002-07-31 2005-12-22 Viktor Menart Process for the production of a heterologous protein

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Also Published As

Publication number Publication date
AU2011223627B2 (en) 2015-06-18
RU2573909C2 (ru) 2016-01-27
PE20130557A1 (es) 2013-05-19
JP5840148B2 (ja) 2016-01-06
EP2542574A2 (en) 2013-01-09
ES2639398T3 (es) 2017-10-26
US20160032345A1 (en) 2016-02-04
CN102906108A (zh) 2013-01-30
US9611499B2 (en) 2017-04-04
EP2542574A4 (en) 2014-01-29
CN102906108B (zh) 2016-01-20
NZ602255A (en) 2014-04-30
CA2791361A1 (en) 2011-09-09
JP2013524775A (ja) 2013-06-20
AU2011223627A1 (en) 2012-09-27
US20110217784A1 (en) 2011-09-08
CL2012002378A1 (es) 2014-01-10
WO2011109556A3 (en) 2012-01-12
CA2791361C (en) 2018-06-12
MX2012010148A (es) 2013-01-22
KR20130018743A (ko) 2013-02-25
EP2542574B1 (en) 2017-08-09
US9187543B2 (en) 2015-11-17
CO6592086A2 (es) 2013-01-02
BR112012022292A2 (pt) 2017-01-10
RU2012141653A (ru) 2014-04-10
WO2011109556A2 (en) 2011-09-09

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