KR101848945B1 - 개선된 세포 배양 배지 - Google Patents

개선된 세포 배양 배지 Download PDF

Info

Publication number
KR101848945B1
KR101848945B1 KR1020127030708A KR20127030708A KR101848945B1 KR 101848945 B1 KR101848945 B1 KR 101848945B1 KR 1020127030708 A KR1020127030708 A KR 1020127030708A KR 20127030708 A KR20127030708 A KR 20127030708A KR 101848945 B1 KR101848945 B1 KR 101848945B1
Authority
KR
South Korea
Prior art keywords
cell culture
medium
polypeptide
production
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020127030708A
Other languages
English (en)
Korean (ko)
Other versions
KR20130098869A (ko
Inventor
볼프강 에른스트 구스타프 부다흐
엘렌느 쎄. 샤쌩
커스틴 도르쉬
Original Assignee
노파르티스 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44276356&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101848945(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 노파르티스 아게 filed Critical 노파르티스 아게
Publication of KR20130098869A publication Critical patent/KR20130098869A/ko
Application granted granted Critical
Publication of KR101848945B1 publication Critical patent/KR101848945B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020127030708A 2010-04-26 2011-04-25 개선된 세포 배양 배지 Active KR101848945B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783710P 2010-04-26 2010-04-26
US61/327,837 2010-04-26
PCT/EP2011/056509 WO2011134921A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
KR20130098869A KR20130098869A (ko) 2013-09-05
KR101848945B1 true KR101848945B1 (ko) 2018-04-16

Family

ID=44276356

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020127030708A Active KR101848945B1 (ko) 2010-04-26 2011-04-25 개선된 세포 배양 배지

Country Status (14)

Country Link
US (2) US8986957B2 (OSRAM)
EP (2) EP2563903B1 (OSRAM)
JP (2) JP6113653B2 (OSRAM)
KR (1) KR101848945B1 (OSRAM)
CN (2) CN102858953B (OSRAM)
AU (1) AU2011246504B2 (OSRAM)
BR (1) BR112012027434B1 (OSRAM)
CA (1) CA2797140C (OSRAM)
ES (1) ES2755907T3 (OSRAM)
IL (1) IL222453B (OSRAM)
MX (1) MX2012012525A (OSRAM)
RU (1) RU2563353C2 (OSRAM)
SG (1) SG185037A1 (OSRAM)
WO (1) WO2011134921A1 (OSRAM)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012027434B1 (pt) 2010-04-26 2020-09-29 Novartis Ag Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção
WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
JP2015531244A (ja) 2012-10-15 2015-11-02 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ タンパク質を製造するための哺乳動物細胞培養方法
CA2943667C (en) * 2014-01-29 2022-11-08 Lg Life Sciences Ltd. Method for modulating galactosylation of recombinant protein through optimization of culture medium
WO2015155975A1 (ja) * 2014-04-07 2015-10-15 東洋製罐グループホールディングス株式会社 動物細胞用培養液、及び培養容器
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
KR20240093910A (ko) 2015-04-01 2024-06-24 베링거 인겔하임 인터내셔날 게엠베하 세포 배양 배지
WO2016192830A1 (en) * 2015-05-29 2016-12-08 Merck Patent Gmbh Deep eutectic solvents and/or ionic liquids in cell culture media
DK3303554T3 (da) * 2015-05-29 2020-08-31 Merck Patent Gmbh Dybe eutektiske opløsningsmidler og/eller ioniske væsker som fodringsmedie
WO2016207721A1 (en) 2015-06-25 2016-12-29 University Of Limerick Mechanical device for generating combinatorial library
WO2017083224A1 (en) * 2015-11-09 2017-05-18 Bristol-Myers Squibb Company Methods to manipulate quality attributes of polypeptides produced in cho cells
KR20190005966A (ko) * 2016-05-10 2019-01-16 제넨테크, 인크. 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을 감소시키기 위한 방법
KR102893388B1 (ko) * 2016-10-31 2025-11-28 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
KR20220097421A (ko) 2019-11-05 2022-07-07 아지노모토 가부시키가이샤 단백질의 제조 방법
CA3167733A1 (en) * 2020-01-14 2021-07-22 Ajinomoto Co., Inc. High-density cell culture method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030087372A1 (en) * 2001-06-13 2003-05-08 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767704A (en) * 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US5266479A (en) * 1989-04-12 1993-11-30 Board Of Regents, The University Of Texas System High calcium chemically defined culture medium
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
WO1992015670A1 (en) * 1991-02-28 1992-09-17 Hyclone Laboratories, Inc. Blended bovine sera cellular growth media and their methods of production and use
GB9118664D0 (en) 1991-08-30 1991-10-16 Celltech Ltd Cell culture
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
JP2000507812A (ja) * 1996-03-12 2000-06-27 ライフ テクノロジーズ，インコーポレイテッド 造血細胞培養栄養補充成分
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
JP4543402B2 (ja) 1996-10-10 2010-09-15 ライフ テクノロジーズ コーポレーション 植物由来栄養素を含む動物細胞培養培地
US6692961B1 (en) * 1996-10-11 2004-02-17 Invitrogen Corporation Defined systems for epithelial cell culture and use thereof
US20060286668A1 (en) * 1999-04-30 2006-12-21 Invitrogen Corporation Animal-cell culture media comprising non-animal or plant-derived nutrients
JP4972261B2 (ja) 1999-08-27 2012-07-11 ライフ テクノロジーズ コーポレーション 金属結合化合物および細胞培養培地組成物中でのそれらの使用
US20030096414A1 (en) 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
HUP0402158A2 (hu) 2001-10-02 2005-01-28 Novo Nordisk Health Care Ag Rekombináns fehérjék előállítási eljárása eukarióta sejtekben
AU2004280064A1 (en) 2003-10-09 2005-04-21 Kyowa Hakko Kirin Co., Ltd. Genomically modified cell neutralized to serum-free system
JP4740138B2 (ja) 2003-10-10 2011-08-03 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 真核生物細胞におけるポリペプチドの大規模生産方法及びそれに適した培養容器
TWI374935B (en) 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
US7300773B2 (en) 2004-08-27 2007-11-27 Wyeth Research Ireland Limited Production of TNFR-Ig
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
WO2006050050A2 (en) * 2004-10-29 2006-05-11 Centocor, Inc. Chemically defined media compositions
JP4833991B2 (ja) * 2004-11-02 2011-12-07 アレス トレーディング ソシエテ アノニム 哺乳動物細胞のための無血清細胞培養培地
TWI369401B (en) 2005-07-05 2012-08-01 Ares Trading Sa Serum-free culture medium for the production of recombinant gonadotropins
US20070231895A1 (en) 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
SI2522717T1 (sl) 2006-01-04 2014-05-30 Baxter International Inc. Mediji celične kulture brez oligopeptidov
US8192951B2 (en) 2006-11-03 2012-06-05 Wyeth Llc Glycolysis-inhibiting substances in cell culture
FR2910024B1 (fr) 2006-12-19 2009-03-27 Agronomique Inst Nat Rech Milieu de culture synthetique chimiquement defini.
WO2008141207A1 (en) 2007-05-11 2008-11-20 Amgen Inc. Improved feed media
ES2695999T3 (es) 2007-10-12 2019-01-11 Hoffmann La Roche Expresión de proteínas a partir de múltiples ácidos nucleicos
JP2011516086A (ja) * 2008-04-18 2011-05-26 上海中信国健薬業股▲ふん▼有限公司 濃縮培養液及びその使用方法
CN101381702A (zh) * 2008-09-10 2009-03-11 吉林省赛达生物科技有限公司 无动物组分细胞培养基
EP2342318A1 (en) * 2008-09-26 2011-07-13 Schering Corporation High titer antibody production
BR112012027434B1 (pt) 2010-04-26 2020-09-29 Novartis Ag Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção
CA2795461C (en) 2010-04-26 2018-04-24 Novartis Ag Improved cell cultivation process
PT2563904E (pt) 2010-04-26 2015-05-12 Novartis Ag Meio de cultura de células aperfeiçoado

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030087372A1 (en) * 2001-06-13 2003-05-08 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells

Also Published As

Publication number Publication date
AU2011246504A1 (en) 2012-11-22
JP6113653B2 (ja) 2017-04-12
AU2011246504B2 (en) 2013-09-26
CA2797140A1 (en) 2011-11-03
EP3599276A1 (en) 2020-01-29
SG185037A1 (en) 2012-11-29
US9243224B2 (en) 2016-01-26
JP2013524826A (ja) 2013-06-20
CA2797140C (en) 2018-01-23
EP2563903A1 (en) 2013-03-06
CN105331659B (zh) 2019-07-16
BR112012027434A2 (pt) 2015-09-22
WO2011134921A1 (en) 2011-11-03
US20130102032A1 (en) 2013-04-25
US8986957B2 (en) 2015-03-24
RU2012150281A (ru) 2014-06-10
CN102858953B (zh) 2015-09-09
JP2016052310A (ja) 2016-04-14
KR20130098869A (ko) 2013-09-05
CN105331659A (zh) 2016-02-17
US20150218509A1 (en) 2015-08-06
BR112012027434B1 (pt) 2020-09-29
RU2563353C2 (ru) 2015-09-20
IL222453B (en) 2018-11-29
IL222453A0 (en) 2012-12-31
EP2563903B1 (en) 2019-08-21
CN102858953A (zh) 2013-01-02
ES2755907T3 (es) 2020-04-24
MX2012012525A (es) 2012-11-23

Similar Documents

Publication Publication Date Title
JP6113653B2 (ja) 改良型細胞培養培地
JP6603628B2 (ja) 改良型細胞培養培地
KR101828624B1 (ko) 개선된 세포 배양 방법
WO2001044442A1 (en) Arginine-enriched medium composition used for mass-producing recombinant protein in animal cell culture
RU2798069C2 (ru) Улучшенная среда для культивирования клеток

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

D14-X000 Search report completed

St.27 status event code: A-1-2-D10-D14-srh-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E601 Decision to refuse application
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T13-X000 Administrative time limit extension granted

St.27 status event code: U-3-3-T10-T13-oth-X000

T13-X000 Administrative time limit extension granted

St.27 status event code: U-3-3-T10-T13-oth-X000

AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PX0901 Re-examination

St.27 status event code: A-2-3-E10-E12-rex-PX0901

PX0701 Decision of registration after re-examination

St.27 status event code: A-3-4-F10-F13-rex-PX0701

X701 Decision to grant (after re-examination)
R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

R18-X000 Changes to party contact information recorded

St.27 status event code: A-5-5-R10-R18-oth-X000

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 8