KR101848250B1 - Composition for anti-inflammatory, skin whitening and anti-obesity comprising bamboo leaf extract as effective component - Google Patents

Abstract

The bamboo-leaf extract of the present invention is extracted by a bamboo-leaf extraction method including an enzyme treatment and an ultra-high pressure homogenization treatment. The extraction method solubilizes insoluble fibrous components to increase extraction yield and physiological activity, In terms of competitiveness can be secured.
In addition, the anti-inflammatory, skin whitening and anti-obesity compositions containing the bamboo leaf extract of the present invention as an active ingredient inhibit NO production, inhibit melanin production, inhibit fat accumulation, As well as functional cosmetics for skin whitening, as well as pharmaceutical compositions for preventing or treating inflammation, obesity and skin pigmentation diseases.

Description

TECHNICAL FIELD The present invention relates to a composition for anti-inflammation, skin whitening and anti-obesity comprising an extract of bamboo leaf as an active ingredient and a composition for anti-inflammation, skin whitening and anti-obesity comprising bamboo leaf extract as effective component.

The present invention relates to a composition for antiinflammation, skin whitening and anti-obesity comprising bamboo leaf extract as an active ingredient.

The color of human skin, hair and eyes is determined by melanin, carotene and hemoglobin. In particular, melanin is the most important factor in determining skin color, and skin color is determined by the amount and nature of melanin and its degree of distribution. The pigment of human skin or hair protects skin and hair from the harmful effects of sunlight, especially ultraviolet rays. For example, people who lack these pigments are very susceptible to sunlight and are susceptible to burns, and the likelihood of developing skin cancer at a young age is high. Short wavelength ultraviolet (290-320 nm) and carcinogens are known to form harmful radicals, such as oxygen radicals, in the skin, and these oxygen radicals attack skin cells and aging skin.

The main function of melanin is to remove these harmful radicals to protect the skin from damage caused by it. Thus, a high amount of melanin means that it has an effective countermeasure to protect the skin from physical or chemical toxicants. Factors that promote the production of melanin include sunlight (ultraviolet rays) as well as estrogen or prostaglandine.

On the other hand, melanin is produced by ultraviolet rays in melanocytes after tyrosinase is converted into dopa (DOPA) and dopachrome by an enzyme called tyrosinase and then subjected to complex oxidation and condensation reaction. At this time, melanin produced is transferred to skin cells, and melanin is lost with epidermal detachment and disappearing circulation.

This process of melanin production is a naturally occurring phenomenon and does not result in overproduction of melanin in normal human skin. However, when the skin responds to external stimuli such as ultraviolet rays, environmental pollution or stress, melanin is overproduced. When melanin is produced in such a way that it is not released to the outside of the skin and remains in the skin, pigmentation occurs and appears as spots and freckles.

Of these external stimuli, ultraviolet light is the largest source of melanin biosynthesis stimuli, which can affect various processes related to melanin production. That is, ultraviolet rays act as an important factor for inducing melanin overgrowth through promoting activity of melanocyte, a melanocyte-producing cell, promoting secretion of melanin biosynthesis stimulating hormone, promoting melanin oxidation, or stimulating tyrosinase activity.

In the prior art, ascorbic acid, which is known as a whitening substance, has low phase stability in a formulation and has a problem of deteriorating its activity over time. Hydroquinone is excellent in its effect, but is proved to be a carcinogenic substance and its use as a cosmetic composition is limited have. In addition, kojic acid is excellent in whitening effect due to its excellent tyrosinase inhibiting ability, but has a problem that phase stability in a formulation is inferior such as ascorbic acid.

Thus, research on whitening substances which are harmless to humans and excellent in formulation stability has been carried out for a long time, and it is expected that natural whitening substances which are harmless to human body and excellent in stability of formulation can be developed by searching for a tyrosinase inhibitory effect function from natural products .

In addition, the inflammatory reaction is one of biological tissue defense responses to external stimuli. It is involved in physiological and pathological processes of various diseases, and is a mechanism to repair or regenerate damaged tissue by external stimuli. These inflammatory responses are characterized by physicochemical, chemical and bacteriological changes in various tissues such as skin and joint tissues.

It has been reported that inflammation can cause a variety of intractable diseases such as dementia, cardiovascular disease, cancer, obesity, arthritis, and diabetes due to persistent inflammation reaction such as fever, erythema and edema. When inflammatory reaction occurs, inflammatory mediators such as nitric oxide (NO), prostaglandin E ₂ (PGE ₂ ) and inflammatory cytokines are secreted. Nitric oxide (NO) is an indicator of inflammatory response. It is synthesized by enzyme (NOS). Nitric oxide (NO) formation plays a role in the immune response, but nitric oxide (NO), which is excessively produced by iNOS induced by liposaccharide (LPS) or inflammatory cytokines, induces the inflammatory response, Damage, genetic mutation, nerve damage, and the like.

Conventional anti-inflammatory drugs are mainly classified into steroidal and non-steroidal anti-inflammatory drugs. Most synthetic anti-inflammatory drugs are accompanied with various side effects in addition to the main action. Therefore, there is a desperate need for the development of anti- In particular, early inflammation through inhibition of NO generation has become a major target for the development of anti-inflammatory drugs.

In addition, obesity refers to a case where the body is excessively overgrown, and according to the national health statistics, the prevalence of obesity is increasing from 26.0% in 1998 to 31.4% in 2011. Obesity has been found to be associated with complications of various adult diseases. For example, obesity patients have been reported to have a higher mortality rate compared to those who have normal weight, twice as much in the case of cirrhosis, 1.6 times in the case of cerebrovascular disease and 1.8 times in the case of coronary artery disease.

Currently, obesity treatment methods such as diet, exercise and drug therapy are being tried. Among them, the therapeutic agents for obesity, which are used as drug therapy, include appetite control, obstruction of digestion and absorption of fat, increase of energy consumption, , But there are various side effects.

Xenical (Roche) inhibits fat absorption by inducing lipase inhibition in the pancreas and digestive system, but has a low weight loss effect of only 2 to 3% and is accompanied by frequent side effects such as diarrhea and fatty liver.

In addition, reductil (Abbott) inhibits appetite by inhibiting the reuptake of serotonin and noradrenaline, thereby reducing body weight by 5 ~ 10%. However, there are side effects of cardiovascular diseases such as stroke and myocardial infarction, The EMA and the US Food and Drug Administration (FDA) withdrew from the market with prescription, suspension and voluntary recall recommendations, and were discontinued by the Food and Drug Administration in October 2010 in Korea.

In addition, many of the products developed as anti-obesity drugs are prohibited from selling due to serious side effects. For example, aminophylline has been reported to have widespread adverse effects on the mental nervous system, circulatory system, and digestive system, despite its excellent body fat degradation, and penfluramine, dexfenfluramine, topiramime, or ephedrine, Sales were banned. As such, the value of natural products and medicines with anti-obesity effect is newly emerging due to the limitations of western medicine due to side effects of synthetic drugs and overcoming of chronic diseases.

On the other hand, 12 ~ 13 species of bamboo are distributed in Korea, such as bamboo jungjuk, wangdae, and cotton, and the bamboo leaf is composed of materials beneficial to human body and has been used as herbal medicine since ancient times. Seokbyeo has taste, nature, and is effective in relieving tumor, fever, and confrontation. It is effective for relieving fever, fever, diarrhea, diarrhea, headache, hypertension, dizziness, nervous breakdown, pregnancy anemia, epilepsy, insomnia, Recovery, etc., and bamboo leaves have the effect of promoting the biochemical metabolism in the body, so it is good for the treatment of hypertension and paralysis, and because it contains many essential unsaturated fatty acids linolenic acid and linolenic acid, .

Korean Patent Laid-Open Publication No. 2016-0024243 discloses a method for producing a fermented bamboo extract and a fermented bamboo extract using the same, and Korean Patent Publication No. 1402936 discloses a composition exhibiting an obesity-suppressing activity including a bamboo leaf extract and a gold extract, But the composition for anti-inflammation, skin whitening and anti-obesity containing the bamboo extract of the present invention as an active ingredient has not been disclosed.

The present invention provides a composition for anti-inflammation, skin whitening and anti-obesity comprising bamboo leaf extract as an active ingredient, and the bamboo leaf extract as an active ingredient is obtained by enzymatic treatment And an ultra-high pressure homogenization process, thereby confirming that the inhibition of nitric oxide (NO) production, the production of melanin, the inhibition of tyrosinase activity and the inhibition of fat accumulation are enhanced, thereby completing the present invention.

In order to achieve the above object, the present invention provides an anti-inflammatory and skin whitening agent comprising an extract of bamboo leaf, which is prepared by a method comprising adding an enzyme to a mixture of bamboo leaf powder and water and hydrolyzing the same, The present invention provides a cosmetic composition.

The present invention also provides a pharmaceutical composition for antiinflammation comprising the bamboo shoot extract as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating melanin pigment hyperpigmentation diseases comprising the bamboo shoot extract as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating obesity comprising the bamboo shoot extract as an active ingredient.

The bamboo-leaf extract of the present invention is extracted by a bamboo-leaf extraction method including an enzyme treatment and an ultra-high pressure homogenization treatment. The extraction method solubilizes insoluble fibrous components to increase extraction yield and physiological activity, In terms of competitiveness can be secured.

In addition, the anti-inflammatory, skin whitening and anti-obesity compositions containing the bamboo leaf extract of the present invention as an active ingredient inhibit NO production, inhibit melanin production, inhibit fat accumulation, As well as functional cosmetics for skin whitening, as well as pharmaceutical compositions for preventing or treating inflammation, obesity and skin pigmentation diseases.

FIG. 1 shows the results of confirming the effect of the balsam extract of the present invention on the inhibition of NO production by macrophage LPS.
FIG. 2 shows the results of confirming the effect of the bamboo shoot extract of the present invention on inhibiting melanin biosynthesis.
FIG. 3 shows the results of confirming the effect of the bamboo extract of the present invention on inhibiting the activity of tyrosinase.
4 is a result of confirming the effect of the bamboo extract of the present invention on the inhibition of fat accumulation.
In each figure, statistical significance of the mean value between the experimental groups was p <0.05 and tested by the Duncan multiple test method.

The present invention provides an anti-inflammatory and skin whitening cosmetic composition comprising a bamboo leaf extract prepared by a method comprising the following steps as an active ingredient:

(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;

(b) dissolving the bamboo leaf powder prepared in the step (a) with water and adding an enzyme to hydrolyze the bamboo powder;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the hydrolyzate treated with ethanol in step (c).

In the method for producing the bamboo leaf extract of the present invention, the bamboo leaf in the step (a) is preferably a bamboo leaf collected from any one or more bamboo selected from the group consisting of tubercle bamboo, It is not.

The enzyme of step (b) is preferably a carbohydrate hydrolase or a pectin hydrolyzing enzyme, but is not limited thereto.

The carbohydrate hydrolase is any one or more enzymes selected from the group consisting of arabinase, cellulase, beta-glucanase, hemicellulase and xylanase But is not limited thereto.

The pectin hydrolyzing enzyme is preferably one or more enzymes selected from polygalacturonase and pectin esterase, but is not limited thereto.

In addition, in the method for producing bamboo shoot extract of the present invention, the step (b) is preferably a step of adding 10 to 14 times (w / v) water adjusted to pH 3.5 to 5.5 to bamboo leaf powder, After adding pectinolytic enzyme, hydrolysis can be carried out at 30 to 60 ° C for 1 to 12 hours, more preferably 12 times (v / w) of water adjusted to pH 4.5 in bamboo leaf powder is added to the mixture After enzymatic or pectin degrading enzyme is added, it can be hydrolyzed at 50 ° C for 8 to 12 hours.

The hydrolysis of the bamboo leaf powder under the above conditions can improve the extraction yield by improving the extraction yield of the active material and solubilizing the insoluble matter of the bamboo leaf by solubilization through molecular rearrangement or molecular rearrangement.

In addition, in the method for producing the bamboo shoot extract of the present invention, the step of treating ethanol in the step (c) is preferably a step of adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate, (V / v) of 100% ethanol is added to the hydrolyzate, and the mixture is stirred at 20 to 30 ° C for 2 hours at 180 rpm.

In addition, in the method for producing the bamboo shoot extract of the present invention, the ethanol-treated hydrolyzate may further include a step of centrifuging and recovering the supernatant.

In addition, in the method for producing bamboo shoot extract of the present invention, the ultra-high pressure homogenization of step (d) can be homogenized at 100-120 MPa pressure, and more preferably at 110 MPa pressure.

In the cosmetic composition according to an embodiment of the present invention, the bamboo shoot extract inhibits the production of nitrogen oxides (NO), and the effect of inhibiting the amount of melanin biosynthesis and the activity of tyrosinase can be confirmed.

The cosmetic composition is preferably, but not limited to, at least one of inflammation selected from atopic dermatitis, acne and inflammation caused by insect bites.

In addition, the whitening effect refers to an effect to improve or prevent glaring skin, spots, freckles and dark circles caused by various factors such as exposure to ultraviolet rays, changes in hormone balance, genetic programs, etc., Or an effect of maintaining a beautiful skin with a sense of transparency and an effect of reducing the dullness of the skin to increase gloss and tautness. In general, it is known that the skin, spots, freckles, and dark circles of the skin, stamens, freckles and dark circles are stimulated by melanocytes due to stimulation by ultraviolet rays or changes in hormone balance, and melanin pigment is deposited on the skin. Therefore, if the production of melanin can be inhibited, it is possible to prevent or improve gross skin, spots, freckles and dark circles.

The method for producing the bamboo shoot extract of the present invention is more preferably

(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;

(b) adding Viscozyme or Pectinex to a mixture of balsamic lobster powder prepared in step (a) in which water adjusted to pH 3.5 to 5.5 was added 10 to 14 times (w / v) Followed by hydrolysis at 30 to 60 ° C for 1 to 12 hours;

(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And

(d) homogenizing the ethanol-treated hydrolyzate of step (c) under ultra-high pressure at a pressure of 100 to 120 MPa.

The viscozyme can be obtained from Aspergillus aculeatus- derived arabinase, cellulase, beta-glucanase, hemicellulase, xylanase, (xylanase).

The pectinex is a complex enzyme comprising a polygalacturonase derived from Aspergillus aculeatus .

The cosmetic composition of the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, It can have one formulation, but is not limited thereto.

The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

In addition, the present invention provides a pharmaceutical composition for antiinflammation comprising, as an active ingredient, a bamboo extract prepared by a method comprising the steps of:

(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;

(b) dissolving the bamboo leaf powder prepared in the step (a) with water and adding an enzyme to hydrolyze the bamboo powder;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the hydrolyzate treated with ethanol in step (c).

The bamboo leaf extract, which is an active ingredient of the anti-inflammatory composition of the present invention, is as described above.

The pharmaceutical composition is preferably to ameliorate or treat any one or more inflammation selected from atopic dermatitis, wound, laceration, acne, and inflammation caused by insect bite, but the present invention is not limited thereto.

The pharmaceutical composition of the present invention is preferably, but not limited to, a carrier, an excipient and a diluent in addition to bamboo shoot extract.

The pharmaceutical composition comprising the bamboo shoot extract of the present invention as an active ingredient may be various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of suppositories include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and it is preferable to select the intravenous, intraperitoneal, rectal, intravenous, intramuscular, subcutaneous, intrauterine or intracerebral injection methods during parenteral administration, But is not limited to.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition of the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The dosage of the pharmaceutical composition of the present invention may be varied depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease.

The present invention also provides a pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, which comprises a bamboo shoot extract prepared by a method comprising the steps of:

(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;

(b) dissolving the bamboo leaf powder prepared in the step (a) with water and adding an enzyme to hydrolyze the bamboo powder;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the hydrolyzate treated with ethanol in step (c).

The bark extract, which is an active ingredient of the pharmaceutical composition for preventing or treating melanin pigment superficial disease of the present invention, is as described above.

According to one embodiment of the present invention, the bamboo leaf may be subjected to high-pressure homogenization after carbohydrase or pectin degrading enzyme treatment to increase the yield of bamboo shoot extract, inhibit melanin biosynthesis, and inhibit tyrosinase activity.

The pharmaceutical composition for preventing or treating melanin pigment hyperproliferative disorder comprising the bamboo leaf extract of the present invention as an active ingredient can be prepared according to the manufacturing method of the person skilled in the art, as described above.

In addition, the present invention provides a pharmaceutical composition for preventing or treating obesity, comprising an extract of bamboo shoots produced by a method comprising the steps of:

(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;

(b) dissolving the bamboo leaf powder prepared in the step (a) with water and adding an enzyme to hydrolyze the bamboo powder;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the hydrolyzate treated with ethanol in step (c).

The bark extract, which is an active ingredient of the pharmaceutical composition for preventing or treating obesity of the present invention, is as described above.

According to one embodiment of the present invention, the bamboo leaf was able to increase the yield of the bamboo leaf extract and inhibit fat accumulation by subjecting it to high pressure homogenization after carbohydrase or pectin degrading enzyme treatment.

The pharmaceutical composition for preventing or treating obesity which comprises the bamboo leaf extract of the present invention as an active ingredient can be produced according to the manufacturing method of a person skilled in the art, as described above.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Manufacturing example  1. Manufacture of bamboo leaf extract

 In order to observe the extraction yield and composition of the extract according to the enzyme treatment and the presence of ultra - high pressure homogenization, High pressure homogenization; And enzyme treatment and high pressure homogenization parallel treatment were prepared as follows.

(1) Enzyme treatment

30 ㎖ of distilled water adjusted to pH 4.5 in 2.5 g of pulverized bamboo shoots and 50 units / ㎖ and 500 unit / ㎖ of enzymes, Viscozyme and Pectinex, lt; / RTI &gt; The enzyme was treated at the optimum time (10 hours and 2 hours), and then 70 ml of 100% ethanol was added. After extraction at 180 rpm for 2 hours at room temperature, the mixture was centrifuged at 12,000 rpm for 15 minutes and then filtered.

Viscozyme can be obtained from Aspergillus aculeatus- derived arabinase, Cellulase, Beta-glucanase, hemicellulase, xylanase (e. G. xylanase), and Pectinex is a complex enzyme containing polygalacturonase derived from Aspergillus aculeatus .

(2) Ultra high pressure homogenization treatment

30 ml of distilled water adjusted to pH 4.5 and 70 ml of 100% ethanol were added to 2.5 g of the pulverized bamboo leaf, and the mixture was stirred at 12,000 rpm for 15 minutes. Then, the mixture was filtered to obtain a filtrate. It was passed through an orifice tube twice.

(3) Enzyme treatment and high pressure homogenization parallel treatment

30 ㎖ of distilled water adjusted to pH 4.5 in 2.5 g of pulverized bamboo shoots and 50 units / ㎖ and 500 unit / ㎖ of enzymes, Viscozyme and Pectinex, lt; / RTI &gt; The enzymes were treated at optimal times (10 hours and 2 hours), and 70 ml of 100% ethanol was added. The mixture was extracted at 180 rpm for 2 hours at room temperature (25 ° C), centrifuged at 12,000 rpm for 15 minutes, And then passed through an orifice tube twice under a pressure of 110 MPa.

Example  1. Identification of extraction yield of bamboo shoot extract

Changes in the extraction yields of various enzymes and ultrahigh pressure treatments were measured for the functional materials using the extracts of the leaves.

As a result, the extraction yield was improved when the enzyme treatment was performed, and the extraction yield was improved even under ultrahigh pressure homogenization compared with bamboo leaf powder. When the enzyme treatment and ultrahigh pressure homogenization treatment were combined, the yield enhancement effect was better than that of bamboo leaf powder. Especially, the highest extraction yield was obtained by 198.05% increase of the high pressure homogenization treatment after Viscozyme treatment (Table 1).

Extraction yield (%) of bamboo leaf by enzyme treatment and ultra high pressure homogenization treatment Processing conditions of bamboo leaves Extraction yield Growth rate compared to untreated group (%) Untreated group 5.63 Enzyme V (viscozyme) 10.12 79.75 Enzyme P (pectinex) 12.96 130.19 Ultrahigh pressure homogeneity 15.42 173.89 Enzyme V + Ultra High Pressure Homogeneity 16.78 198.05 Enzyme P + ultrahigh pressure homogeneity 15.36 172.82

In the above table, the untreated group means that 30 ml of distilled water adjusted to pH 4.5 was mixed and stirred in 2.5 g of bamboo shoots.

Example  2. In the macrophage of bamboo shoot extract LPS  (NO) production by nitric oxide

Mouse macrophage RAW264.7 cell line was dispensed in a 96-well plate to a final concentration of 1 x 10 ⁶ cells / ml, and then cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. Then, 400 μg / ml And then treated with LPS (1 μg / ml) for 1 hour and cultured for 24 hours. 100 μl of the cell culture solution and 100 μl of the grease reagent were mixed and reacted at room temperature for 15 minutes. The absorbance was measured at 540 nm using a microplate reader, and a standard curve was prepared using sodium nitrate to calculate NO production amount .

Experimental results showed that NO production induced by LPS decreased during enzyme treatment compared to bamboo leaf powder, and that NO production decreased even under ultrahigh pressure homogenization treatment. In particular, when the enzyme treatment and the ultra-high pressure homogenization treatment were performed in parallel, it was confirmed that LPS-induced NO production was suppressed (FIG. 1).

Example  3. Identification of whitening activity of bamboo shoot extract

3-1. Melanin Biosynthesis amount  Inhibitory effect

In order to confirm the whitening activity of the bamboo shoot extract, the amount of melanin biosynthesis was measured. The B16F10 cell culture was inoculated to a 1 × 10 ⁵ cells / ㎖ in 12-well (well), and 24 hours later jukyeop extract to each well (100 ㎍ / ㎖) and arbutin handle (100 ㎍ / ㎖) for 48 hours. Respectively. After the treatment, the cells were washed twice with PBS (phosphate buffered saline) and centrifuged to make cell precipitate. 200 μl of 1 N NaOH solution containing 10% DMSO (dimethyl sulfoxide) was added and dissolved at 60 ° C for 1 hour. Absorbance was measured at 405 nm.

The melanin biosynthesis amount of the bamboo shoot extract of the present invention prepared by the method comprising the enzymatic treatment and the ultra-high pressure homogenization step was analyzed to be similar to that of the positive control group arbutin (Ar), and the enzyme treatment and high pressure homogenization Bark extracts showed the highest inhibitory effect on melanin production.

In addition, it was confirmed that the bamboo leaf extract which was subjected to the enzyme treatment or the ultra-high pressure homogenization step had an effect of further reducing the melanin production as compared with bamboo leaf powder (bamboo leaf extract not containing the enzyme treatment or the ultra-high pressure homogenization step) (FIG.

3-2. Inhibitory Effect of Tyrosinase on Activity

In order to confirm the whitening activity of bamboo shoot extract, tyrosinase inhibitory activity was measured. In order to determine tyrosinase inhibitory activity, 160 μL of a substrate solution obtained by dissolving 10 mM L-3,4-dihydroxypheny-lalanine (L-DOPA) in 1 mL of a 0.1 M sodium phosphate buffer (pH 6.8) mu] L of the mixture was reacted at 37 [deg.] C for 1 hour to measure DOPA chrome produced in the reaction solution at 475 nm.

As a result, the efficacy was similar to that of arbutin (Ar), which is a positive control, and the extract of bamboo leaves treated with both enzyme treatment and ultra - high pressure homogenization showed the most effective inhibitory activity of tyrosinase.

In addition, it was confirmed that the bamboo leaf extract which has undergone the enzymatic treatment or the ultra-high pressure homogenization step has an effect of further inhibiting the activity of tyrosinase as compared with the bamboo powder (bamboo leaf extract containing no enzymatic treatment or ultra-high pressure homogenization step) 3).

Example  4. bamboo extract Anti-obesity  Check efficacy

To determine the degree of differentiation of 3T3-L1 cells, a lipogenic precursor cell line, the cells were stained with oil red-O. In order to observe the differentiated cells, the cultured cells were fixed with 10% formalin / PBS for 5 minutes at room temperature, and then the cells were washed once with 60% isopropanol and dried. Then, oil red-oak was added for 30 minutes, stained, and washed 4 times with distilled water. Then, fat droplets were observed under a microscope while containing distilled water. After observation, 99% isopropanol was added and dissolved at 180 rpm for 10 minutes and then measured at 520 nm.

According to the above-mentioned method, in order to measure the fat accumulation inhibitory ability of 3T3-L1 cells of the bamboo shoot extract by the enzyme treatment and the ultra-high pressure homogenization process of the present invention, it was diluted with the same dilution ratio and found that fat accumulation Inhibitory effect, and showed the effect of suppressing fat accumulation even under ultrahigh pressure homogenization treatment.

In particular, it was confirmed that when the bamboo extract was treated with the enzyme treatment and the ultrahigh pressure homogenization treatment, the fat accumulation inhibitory effect was further enhanced (FIG. 4).

Claims (9)

An anti-inflammatory and skin whitening cosmetic composition comprising a bamboo leaf extract prepared by a method comprising the steps of:
(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;
(b) dissolving the balsamic acid powder prepared in step (a) in water, adding a carbohydrate hydrolase or pectin hydrolase, and hydrolyzing the balsaw powder;
(c) treating the hydrolyzate of step (b) with 100% ethanol; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa. The cosmetic composition for anti-inflammatory and skin whitening according to claim 1, wherein the bamboo leaf is a bamboo leaf collected from any one or more bamboo selected from the group consisting of tubular carmium, goryeo sari, delete delete The method of claim 1, wherein the bamboo leaf extract
(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;
(b) adding a carbohydrate hydrolase or a pectin hydrolase to a mixture of the balsamic acid powder prepared in the step (a) in which water adjusted to a pH of 3.5 to 5.5 is added 10 to 14 times (w / v) Hydrolyzing at &lt; RTI ID = 0.0 &gt; 60 C &lt; / RTI &gt; for 1 to 12 hours;
(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa under ultra-high pressure. The cosmetic composition for anti-inflammatory and skin whitening according to claim 1, wherein the cosmetic composition improves at least one inflammation selected from atopic dermatitis, acne and inflammation caused by insect bites. An antiinflammatory pharmaceutical composition comprising a bamboo leaf extract prepared by a method comprising the steps of:
(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;
(b) adding a carbohydrate hydrolase or a pectin hydrolase to the mixture of the balsamic acid powder prepared in the step (a), and then hydrolyzing the mixture;
(c) treating the hydrolyzate of step (b) with 100% ethanol; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa. A pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder comprising an extract of bamboo shoots prepared by a method comprising the steps of:
(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;
(b) dissolving the bamboo leaf powder prepared in the step (a) with water and adding hydrolysis by adding a carbohydrate hydrolase or pectin hydrolase;
(c) treating the hydrolyzate of step (b) with 100% ethanol; And
(d) homogenizing the hydrolyzate treated with ethanol in step (c) under a pressure of 100 to 120 MPa. A pharmaceutical composition for preventing or treating obesity comprising bamboo extracts prepared by a method comprising the steps of:
(a) drying a bamboo leaf and then pulverizing the bamboo leaf to produce a bamboo leaf powder;
(b) dissolving the bamboo leaf powder prepared in the step (a) with water, and hydrolyzing the mixture by adding a carbohydrate hydrolase or a pectin hydrolase;
(c) treating the hydrolyzate of step (b) with 100% ethanol; And
(d) homogenizing the hydrolyzate treated with ethanol in step (c) under a pressure of 100 to 120 MPa.

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