KR102603824B1 - Composition for anti-obesity with synergistic effect - Google Patents

Abstract

The present invention relates to an anti-obesity composition containing Goryanggang extract and bamboo leaf extract. The combination of Goryanggang and bamboo leaf extract has excellent effects on inhibiting adipocyte differentiation and changes in blood lipids, and furthermore includes extracts of fenugreek, Gilge, etc. It relates to an anti-obesity composition further comprising.

Description

Anti-obesity composition with synergistic effect {Composition for anti-obesity with synergistic effect}

The present invention relates to an anti-obesity composition containing Goryanggang extract and bamboo leaf extract. The combination of Goryanggang and bamboo leaf extract has excellent effects on inhibiting adipocyte differentiation and changes in blood lipids, and furthermore includes extracts of fenugreek, Gilge, etc. It relates to an anti-obesity composition further comprising.

Obesity is a condition in which excessive fat tissue is accumulated in the body. People who are obese generally weigh a lot, but even people with a lot of muscle can gain a lot of weight even if they are not obese, so obesity is defined as a condition in which there is excess fat tissue in the body. do. If nutrients are consumed excessively compared to energy consumption over a long period of time, obesity is induced due to energy imbalance, and in addition, hormonal changes, genetics, mental health problems, and socioeconomic factors are complexly related. The number of obese people is increasing worldwide, and the severity of obesity has recently emerged as an important issue as it has been pointed out as an important factor causing adult diseases.

Meanwhile, type 2 diabetes is closely related to obesity. PPAR (peroxisome proliferation activated receptor), one of the transcription factors, is known to regulate gene expression of enzymes related to lipid and glucose homeostasis and promote fat tissue production. Additionally, it is known that these changes in PPAR cause obesity. In addition, PPARγ is a major transcription factor that is expressed in adipocytes and changes preadipocytes into adipocytes, and is known to induce obesity through this mechanism.

In order to prevent and improve obesity and maintain a healthy weight, consistent management throughout life is necessary through a balanced diet and regular exercise. In particular, once fat cells are created, their size can be reduced, but it is impossible to remove them naturally and they remain in the body permanently, so it is important to prevent them from becoming obese from the beginning. To prevent, improve, or treat obesity, methods such as reducing calories consumed, increasing activity and amount of exercise, and taking drugs that reduce fat absorption are used.

Current treatments for obesity include fenfluramine, which inhibits the serotonergic nervous system, ephedrine and caffeine, which act on the noradrenergic nervous system, sibutramine, which acts simultaneously on the serotonin and noradrenergic nervous system, and Orly, which reduces fat absorption by inhibiting lipase produced in the pancreas. There are medications such as Start. However, among the previously used drugs, fenfluramine has been banned due to side effects such as primary pulmonary hypertension and heart valve lesions, and other drugs have also caused problems such as decreased blood pressure and lactic acidemia, so they are not used in patients with heart failure or renal disease. There is a problem that cannot be done.

In the case of drugs for dieting, they are divided into two mechanisms: suppressing fat accumulation (appetite suppressants, lipogenesis inhibitors, etc.) or increasing fat activity (thermogenesis, lipolysis, etc.). Although it can be prescribed and taken after treatment, side effects such as digestive problems and insomnia occur, so the need to develop natural raw materials that can replace them is increasing.

It was confirmed that the combination of Goryangkang and bamboo leaves of the present invention is an effective ingredient that can suppress obesity, and further experiments were conducted to increase its efficacy to develop a combination excellent for anti-obesity.

The present invention seeks to provide a highly effective anti-obesity composition.

The present invention is an anti-obesity composition containing Goryanggang extract and bamboo leaf extract as active ingredients. The ratio of the extract may be 50 to 150 parts by weight of the bamboo leaf extract, based on 100 parts by weight of the Goryang River extract. Preferably, it is better to use the same ratio as 100 parts by weight of the bamboo leaf extract.

In addition, the present invention can increase anti-obesity efficiency by further including fenugreek extract and Gilge extract. The amount of the fenugreek extract or Gilge extract is preferably 25 to 75 parts by weight, and more preferably about 50 parts by weight, based on 100 parts by weight of the Goryangkang extract.

In addition, it is more effective to administer the composition at a dose of 3 to 9 mg/kg based on the Koryanggang extract.

The anti-obesity composition of the present invention can maximize the anti-obesity function through synergistic effects between compositions.

Figure 1 is a graph showing the results of an experiment on preadipocyte growth inhibition.
Figure 2 is a graph showing the results of an experiment for inhibiting the growth of differentiated adipocytes.
Figure 3 is a graph showing changes in body weight as a result of experiments on animals administered in an example of the present invention.

Alpinia officinarum Hance used in the present invention is a perennial herb belonging to the ginger family with a height of about 1 meter, and the rhizome is dug up and dried in the fall. Galangal, also known as Asian ginger, is somewhat different from common ginger ( Zingiber officinale Roscoe). Ordinary ginger (health) does not show as much effect as Goryanggang. In traditional medicine, it is said to be effective in treating abdominal pain, vomiting, and diarrhea. It is also highly effective in treating abdominal pain and diarrhea caused by eating cold food, drinks, or fruit. It is also said to be effective in treating weak kidney function, loss of stamina due to poor circulation, diarrhea, and dysentery. The main components of essential oil include cineol, methylcinnamate, eugenol, pinene, and cadinene. In addition, it contains flavonoids such as galangin, kaempferide, kaempferol, quercetin, isoramnetin, and galangin-3-methyl ether, and as a sour ingredient, galangol, etc. It is known that its main pharmacological actions include promoting gastric juice secretion, analgesic action, anti-oxidation effect, anti-thrombotic action, anti-coagulant action, and improvement of peripheral circulation.

While researching the anti-obesity effect of Chazugi, the present inventors confirmed that Goryang-gang also has an excellent anti-obesity effect. In particular, the combination of Goryang-gang and bamboo leaves has an excellent anti-obesity effect. Furthermore, the combination of these with Chazugi also showed quite excellent effects. The present inventors confirmed that Goryangkang and bamboo leaf extracts have effects such as inhibition of adipocyte differentiation and changes in blood lipids, and proposed an anti-obesity composition containing them as an active ingredient.

In addition, the aim is to identify combinations of additional extracts that increase the anti-obesity effect and provide an anti-obesity composition further comprising them. Furthermore, the effective usage amount was confirmed.

In the case of Goryanggang extract, it showed a similar effect in inhibiting the growth of preadipocytes as Garcinia Cambogia extract, a health functional food ingredient recognized for its functionality in inhibiting body fat accumulation. In the case of the bamboo leaves used in the present invention, there was some effect, but it did not show a satisfactory level of effect. However, when Goryanggang extract and bamboo leaf extract were used together, the effect was much better than Garcinia Cambogia extract. The effect of inhibiting the growth of differentiated adipocytes showed similar results, and actual animal experiments also showed similar results.

Bamboo leaves are the dried leaves of Phyllostachys bambusoides Siebold & Zucc. Chamdae grows in the central and southern regions of our country. Pick green leaves in the summer and dry them in a well-ventilated shade. The taste is bitter and the nature is cold. It acts on the heart meridian, menopause, stomach meridian, and liver meridian. It reduces fever, relieves chest tightness, removes phlegm, and soothes spasms. It is used for fever, chest tightness, thirst, vomiting due to stomach fever, coughing with phlegm, shortness of breath, liver, laryngitis, and tongue sores.

Based on 100 parts by weight of Goryang River extract, bamboo leaves can be used within the range of 50 to 150 parts by weight. If too little is used, the synergistic efficiency is not good, and if too much is used more than necessary, the effect does not increase proportionally. The preferred amount of use is 100 parts by weight, and it was effective to use it at a similar specific gravity.

Meanwhile, the present inventors paid attention to fenugreek and fennel root from an oriental medicine perspective, selected them, and conducted experiments. Although fenugreek and apricot root did not have an effective anti-obesity effect on their own, they showed significant effects when used together. In the case of fenugreek and Giljung, as an auxiliary synergistic ingredient, it is appropriate to use about half of the Goryanggang extract. Fenugreek and Gilgeung can be used in an amount of 25 to 75 parts by weight based on 100 parts by weight of Goryanggang extract, and more preferably about 50 parts by weight. If too little is used, the auxiliary synergistic effect is not significant, and if more than necessary is used, the effect does not increase proportionally.

The scientific name of fenugreek (Trigonellae Semen) is Trigonella foenum-graecum L and is also called Fenugreek. Its place of origin is India, China, Southeast Asia, Europe, Egypt, Sudan, the United States, Lebanon, Argentina, etc. Fenugreek seeds are known to be usually dried in the sun and used for medicinal purposes. Fenugreek is warm and non-toxic, so it has been used in Africa, the Middle East, and India to treat diseases of the bladder and kidneys for a long time. It is also used to treat people with cold sweats or a full stomach. In the herbal medicine class, it is used to treat men's waists. It is said to treat aches, cold knees, and wet scrotum, eliminate all symptoms of lethargy and numbness, treat symptoms of frequent urination, and treat symptoms of a cold stomach. Recently, it has been reported to be effective in maintaining the balance of blood sugar and insulin in the body, so research is actively underway.

Gilgyeong is a medicinal herb made by removing the roots or pericarp of bellflower ( Platycodon grandiflorum A. De Candolle) of the Campanula family. The name Gilgyeong was given because the roots are hard and straight. This medicine has a slight odor and a bitter and bitter taste, but its properties are flat and not biased towards one side. Gilgyeong acts on the lungs to treat symptoms of excessive seawater and phlegm and difficulty in breathing. It clears the lungs, relieves stuffy chest, and relieves cold energy in the stomach, stopping coughing and removing phlegm. It is also used when there is edema throughout the body and the amount of urine is small due to difficulty in urinating. It is used for sore throat, cough due to cold, phlegm, nasal congestion, asthma, bronchitis, pleurisy, headache, chills, tonsillitis, etc. Expectorant, blood sugar-lowering, cholesterol-lowering, and antibacterial properties have been reported as pharmacological effects.

The appearance is irregularly elongated, spindle-shaped or conical, sometimes branched, and the outer surface is grey-brown, light brown or white. The tap root has a concave mark at the upper end where the stem was removed, and there are thin horizontal wrinkles and vertical grooves near it, and some are slightly curved. Most of the roots, except the upper part, have rough vertical wrinkles, horizontal grooves, and horizontal lines in the shape of a lanceolate tree. The quality is strong but easy to break. The broken surface is not fibrous and sometimes has large gaps. Other names include Gilgyeonggeun, Gyeongcho, Gogyeong, Gogilgyeong, Liyeo, Bangdo, Baekyak, and Fuho. etc.

The core composition of the present invention is a combination of kaoliang steel and bamboo leaves. Their combination has a synergistic effect on the anti-obesity effect. On the other hand, fenugreek and apricot root showed significant excellent effects when added together. In the case of Goryanggang, a key active ingredient, the actual effect was better when administered at a dose of 3 to 9 mg/kg based on Goryanggang extract.

Meanwhile, in the present invention, the term “extract” refers to an active ingredient isolated from a natural product. The extract can be obtained through an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes the extract, its dry powder, or any form formulated using it. In the present invention, the Gilge extract, etc. can be extracted and used using water, an organic solvent, or a mixed solvent thereof. Preferably, the extract is soluble in lower alcohols having 1 to 4 carbon atoms or a mixed solvent thereof, and extraction using ethanol is particularly preferable. The extracted liquid can be used directly or after being concentrated and/or dried. When extracting using an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide An organic solvent such as (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof can be used, and the active ingredients of the herbal medicine can be extracted at room temperature or by heating under conditions where the active ingredients are not destroyed or minimized. The degree of extraction and loss of the active ingredient of the drug may vary depending on the organic solvent being extracted, so select and use an appropriate organic solvent. The extraction method is not particularly limited and includes, for example, cold needle extraction, ultrasonic extraction, and reflux cooling extraction. Filtration is a process of removing floating solid particles from the extract. The particles may be filtered using cotton, nylon, etc., or ultrafiltration, cryofiltration, centrifugation, etc. may be used, but are not limited thereto. Methods such as reduced pressure concentration and reverse osmosis concentration may be used to concentrate the extract. The drying step after concentration includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, and infrared drying. In some cases, a process of grinding the final dried extract may be added. Additionally, the extract can be subjected to further fractionation processes.

Hereinafter, the present invention will be described in more detail through examples and test examples. However, these examples are used for illustrative purposes to aid understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

<Example 1>

2 kg of Goryanggang purchased from a Seoul herbal medicine store was dried in the shade and at room temperature for 5 days and ground. The pulverized chazuki was immersed in 30 liters of 40% alcohol (ethanol) and extracted at 50°C for 24 hours. This was filtered through filter paper, dried and concentrated under reduced pressure to obtain a total of 310 g of extract.

<Example 2>

Extraction was performed in the same manner as in Example 1 using 2 kg of bamboo leaves purchased from a Seoul herbal medicine store, and a total of 290 g of extract was obtained.

<Example 3>

Extraction was performed in the same manner using 3 kg of fenugreek purchased from a Seoul herbal medicine store, and a total of 530 g of extract was obtained.

<Example 4>

Extraction was performed in the same manner using 3 kg of Gilgyeong purchased from a Seoul herbal medicine store, and a total of 510 g of extract was obtained.

<Example 5>

The composition was prepared in a ratio of 100 parts by weight of the bamboo leaf extract of Example 2 to 100 parts by weight of the Goryang River extract of Example 1.

<Example 6>

The composition was prepared at a ratio of 50 parts by weight of the fenugreek extract of Example 3 to 100 parts by weight of the Goryanggang extract of Example 1.

<Example 7>

The composition was prepared at a ratio of 50 parts by weight of the Gilge extract of Example 4 to 100 parts by weight of the Koryanggang extract of Example 1.

<Example 8>

For Example 5, the composition was composed of 50 parts by weight of the fenugreek extract of Example 3 based on 100 parts by weight of the Koryanggang extract.

<Example 9>

For Example 5, the composition was composed of 50 parts by weight of the Gilge extract of Example 4 based on 100 parts by weight of the Koryanggang extract.

<Example 10>

For Example 5, based on 100 parts by weight of Goryanggang extract, the composition was composed of 50 parts by weight of fenugreek of Example 3 and 50 parts by weight of Gilge extract of Example 4.

<Toxicity evaluation>

MTT analysis was performed to confirm the effect of the examples on the survival rate of 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were seeded in 96-well plates at a concentration of 1Х10 ⁵ cells/ml. Afterwards, cells differentiated through differentiation induction were treated with DW as examples and negative controls at a concentration of 50 to 250 ppm and cultured for 2 days. After 2 days, the medium was replaced with 0.5 mg/ml MTT reagent and cultured for 1 hour. Afterwards, the medium was discarded, 100 μl of DMSO was dispensed to dissolve the produced formazan, and the absorbance was measured at a wavelength of 540 nm.

There was no significant difference compared to the negative control group at all concentrations, so it was judged that cytotoxicity was not a problem.

<Experiment to inhibit growth of preadipocytes>

3T3-L1 preadipocytes were incubated at a density of 1x10 ⁴ cells/ml in a 96-well plate. Except for the negative control group (DMSO solvent control group), cells were treated with Garcinia cambogia extract (positive control group) and Examples at a concentration of 100 ppm. Each group was repeated three times and the average value was used. Garcinia cambogia extract is a health functional food ingredient recognized for its functionality in inhibiting body fat accumulation. After the administration process and 48 hours of incubation, the cell growth status was recorded, and the growth inhibitory effect of each test substance on 3T3-L1 preadipocytes was analyzed by MTT analysis.

The results are shown in Figure 1 compared to the control group. Although the Goryanggang extract did not show better results than the positive control Garcinia cambogia extract, it showed almost similar effects. Bamboo leaves, fenugreek, and apricot root themselves had some effect, but did not show significant results. However, in the case of 5, which is a combination of Goryanggang and bamboo leaves, it showed a superior effect than the positive control group, confirming the synergistic effect of these combinations. Also, adding fenugreek and apricot root alone to these combinations did not show a significant change. However, Example 10, in which fenugreek and apricot root were added together, showed the best results.

<Experiment to inhibit growth of differentiated adipocytes>

3T3-L1 cells were incubated at a density of 1x10 ⁵ cells/well in 12-well plates. 3T3-L1 preadipocytes were cultured in medium containing 10% BCS and 1% P/S, and the culture environment was maintained at 37°C and 5% carbon dioxide. Two days after cell confluency was reached (Day 0), the medium was replaced with differentiation induction medium containing 5 μg/ml insulin, 1 dexamethasone, 0.5mM IBMX, and 10% FBS. From Day 2, the medium was replaced with only 5 μg/ml insulin and 10% FBS, and cultured for 8 days, replaced with new medium every two days. The extract was treated with the cells starting from Day 0, and the final concentration applied to the cells was 250 ppm based on the concentration of Chazugi.

The results are shown in Figure 2 compared to the control group. Although the Goryanggang extract did not show better results than the positive control Garcinia Cambogia extract, it showed a relatively similar effect, confirming that the Goryanggang itself was of considerable significance. Excellent effects were shown when Goryanggang and bamboo leaves were used together as in Example 5, and when Goryanggang, bamboo leaves, fenugreek, and Gilgyeong were used together as in Example 10. Overall, it showed a similar trend to the results of the preadipocyte growth inhibition experiment.

<Weight change experiment after inducing obesity>

In this experiment, 8-week-old female B6 mice were used. In this experiment, the groups were divided into normal control group, obese control group, and group administered the extract of the example. This experiment was performed using 5 female mice in each group. During the experiment, except for the normal control group of mice fed a normal-fat diet, mice in other groups were fed a high-fat diet for 8 consecutive weeks to induce obesity. At the same time, the test substances were administered orally daily for 8 weeks. Reflecting the results obtained through the preadipocyte growth inhibition experiment and the differentiated adipocyte growth inhibition experiment, Garcinia cambogia extract was used as a positive control, and the effectiveness of the combination of Examples 1, 5, and 10 was verified. Differences in body weight and body fat between mice within groups were assessed, and each animal's body weight and average food intake were recorded weekly during the experiment.

The final weight change is shown in Figure 3. The normal control group showed a weight gain of approximately 9g, and the obese control group on a high-fat diet gained 33.5g. When garcinia cambogia extract, which was a positive control, was administered, a weight gain of 14.8 g was observed, showing an anti-obesity effect. Meanwhile, Example 1 was somewhat less effective than the positive control group, but showed a similar level of effect. In the case of Example 5 and Example 10, it showed a superior effect than the positive control group. Example 10 showed a similar level of effect to the normal control group.

<Changes in blood biochemical indicators>

Before and 8 weeks after the start of the animal experiment, the animals were fasted (water supplied) for more than 16 hours, then first anesthetized through isoflurane (Baxter, USA) inhalation, and then fasting blood was collected from the inferior vena cava of the abdomen. Total cholesterol (Total-cholesterol; Total-C), triglyceride (TG), free fatty acid (FFA), high-density lipoprotein-cholesterol (HDL-C), and And the amount of non-high-density lipoprotein-cholesterol (nonHDL-cholesterol; nonHDL-C) was measured. The content of all five lipids in the obese control group increased compared to the normal control group. In the case of the positive control group, the content of TG, Total-C, and FFA decreased slightly compared to the obese control group, but did not show much difference. HDL-C showed a similar level, and the content of non-HDL-C significantly decreased. did. In contrast, Example 5 and Example 10 of the present invention showed excellent results overall.

<Sensory evaluation>

Since the composition of the present invention is a safe material that can be used as a food composition, 25 adult men and 25 adult women (in their 40s to 50s) were tested for body weight measurement and subjective feelings of body fat change (fatigue, bowel movement effect). Five adult men and five adult women were assigned to each of the compositions of placebo, Garcinia cambogia extract, Example 1, Example 5, and Example 10. Examples 5 and 10 showed more satisfactory characteristics than the Garcinia cambogia extract used as a positive control.

Claims (4)

An anti-obesity composition containing Goryanggang extract, bamboo leaf extract, fenugreek extract, and Gilge extract as active ingredients, based on 100 parts by weight of Goryanggang extract, 100 parts by weight of bamboo leaf extract, 50 parts by weight of fenugreek extract, and 50 parts by weight of Gilge extract. An anti-obesity composition, characterized in that it is contained in a negative ratio. delete delete delete