KR20210063085A - Food complex composition for Anti-obesity and preparation method - Google Patents

Abstract

According to the present application, disclosed is the anti-obesity effect of a single extract of Cirsium setidens, Reynoutria japonica and Saururus chinensis, and a complex composition blended in a certain ratio thereof, wherein the complex composition of Reynoutria japonica and Saururus chinensis has the most excellent anti-obesity use.

Description

Complex food composition for anti-obesity and its manufacturing method {Food complex composition for Anti-obesity and preparation method}

The present invention relates to a novel new material as a complex composition for reducing body fat and preventing and improving obesity, which includes extracts of Hojang-geun and trifolieae.

Obesity generally refers to an excess of adipose tissue in the body, and is a phenomenon in which energy consumed from food is not in balance with energy consumed through physical activity, and the excess energy is accumulated as body fat. When body fat increases abnormally due to energy imbalance over a long period of time, various metabolic diseases and adult diseases such as diabetes, hyperlipidemia, heart disease, stroke, arteriosclerosis, fatty liver, etc. are induced, which is emerging as a serious social problem not only in the West but also in Korea. .

Obesity is caused by a variety of causes, such as genetic factors, environmental factors caused by a westernized diet, psychological factors caused by stress, and abnormal energy metabolism. And depending on the cause, it can be divided into simple obesity caused by overeating and lack of exercise and symptomatic obesity caused by endocrine, hypothalamus, heredity, and metabolism.

There are two methods for assessing the degree of obesity: measuring body weight and measuring the thickness of skin folds. Generally, obesity is defined as obesity if the obesity index is 25 or higher. Body obesity index (body mass index, BMI) is a value obtained by dividing weight (kg) by the square of height (m). .

Various aspects of research are being conducted worldwide for the development of anti-obesity drugs. Drugs for the treatment of obesity can be broadly divided into fat absorption inhibition, lipolysis and heat generation promotion, appetite and satiety regulation, protein metabolism inhibition, and emotional regulation related to food intake. Representative anti-obesity treatments include Xenical™, which suppresses fat absorption by using oristat as a raw material, and Reductil™, which suppresses appetite by stimulating the sympathetic nervous system using sibutramine as the main ingredient. . However, in the case of Xenical™, side effects such as fatty stools, abdominal pain, vomiting, itching, and liver damage have been reported. In the case of Reductil™, side effects such as headache, anorexia, insomnia, and constipation as well as serious cardiovascular side effects have been reported. Controversy has arisen over the recent tightening of standards for use.

In addition to drug therapy through these anti-obesity drugs, methods for preventing and treating obesity include diet to limit food intake, exercise therapy to increase energy consumption, and psychotherapy, behavioral therapy, and surgical therapy.

As a preferred method of treating obesity, it is suggested as the safest and most effective way to promote energy consumption through exercise and to combine drugs for the treatment of obesity with fewer side effects. However, in the case of drugs for the treatment of obesity, serious side effects have been reported as in the examples of Xenical™ and Reductil™, and clear trust in safety is not supported. Therefore, there is a demand for the development of a substance that exhibits excellent anti-obesity efficacy on the human body while ensuring safety.

Gondree, also called 'Cirsium setidens', is a perennial plant belonging to the Asteraceae family. Common gondree is widely used as food such as greens and kimchi. It is known to be good for diuretic action, diuretic action, anti-inflammatory action, hemostatic action, and venous dilatation.

It is widely known that milk thistle has a direct protective action on hepatocytes and reinforcing energy by stabilizing changes in hepatocyte membranes with the food science oriental medicine efficacy of milk thistle.

In Korean, thistle is called Jobanggasae ('subgye') in Korean, and thistles that grow over 1 meter are called Hanggasae ('daegye') in Korean. Both are used as medicines, but mainly daffodils with better medicinal effects are used. It is not poisonous, has a sweet taste, and has diuretic, detoxifying and anti-inflammatory effects, and controls heat so that it does not interfere with normal blood circulation. It has hemostasis and is applied to various types of bleeding, for example, hematemesis, nosebleeds, gum bleeding, stool bleeding, urine bleeding, and uterine bleeding. In addition, when blood circulation is poor and hardens, causing pain and clots, it helps blood to circulate smoothly and cleans the accumulated clots. Therefore, it is very effective for malignant tumors including bruises, swelling, and boils.

In <Donguibogam>, the thistle is recorded as follows.

"Plain in nature, bitter in taste, and non-toxic. It relieves blood clots, stops vomiting and nosebleeds, and cures swelling, scabies, and ringworm.精) and protects blood. It grows everywhere, the freshly sprouted leaves are plucked in May of the lunar calendar, and the roots are dug up in September and dried in the shade [prime plant]. Designation (地丁) is thistle. It is called 'Hwanghwajijeong (黃化地丁)' and the purple flowers are called 'Jahwajijeong (紫花地丁)', and they all heal all kinds of diseases [Jeongjeon]."

As a study on gondre, which is known to have such efficacy, as in the patent documents described below, research is generally limited to the cooking field, and there is a unfortunate problem that it is not widely used compared to its efficacy.

Conventional patent documents related to gondre include Korean Patent Publication No. 10-2012-0007325, a method for producing gondre namul rice using gondre herb juice, Korean Patent No. 10-0589649, a manufacturing method of gondre namul rice, and Korean Patent Publication No. 10-2010-00039841 There is a method for manufacturing tofu containing hoin gondree, and a method for manufacturing gondree herb block, which is a domestic registered patent No. 10-1214510.

In addition, as a patent document on a composition for preventing or improving hyperlipidemia using a natural plant extract, a natural plant extract composition effective in the recovery and prevention of hyperlipidemia and stroke of Korean Patent No. 10-0795822 using Albania and Schisandra, effective Various technologies are provided, such as a natural tea as an ingredient, a method for manufacturing it, and an antithrombotic and hyperlipidemia inhibitory composition using a natural plant material extract of Korean Patent Application Laid-Open No. 2001-0009590.

On the other hand, Hojanggeun (Polygonum cuspidatum Sieb. et Zucc. = Reynoutria japonica Hou.), a herbaceous perennial plant belonging to the family Knobaceae, refers to the roots or rhizomes of Hojang and related plants. It is also called , Banjang, Santongsun, Banjanggeun, Obudap, Sangan, Bangeun, Unghwangyeon, Tojiyu. Hojanggun is distributed in Korea, Japan, Taiwan, China, etc., and in Korea, it grows in the valleys of Sanya across the country. The rhizome is over 1m in height, and the rhizome extends from the basement to the side, is woody, yellowish-brown, and has clear nodes. Stems grow straight and are hollow in a circular shape. The surface is hairless and there are many red or purple spots. These roots, rhizomes and leaf parts have been used medicinally since ancient times. As the pharmacological action of hojanggeun, it is known as a relieving, diuretic, analgesic, and anti-thickening agent, and has antibacterial and antiviral pharmacological actions.

Cinnamomum cassia Blume, an evergreen tree belonging to the camphor family, is the stem bark of a broiler or other closely related plant with the epidermis and primary cortex removed. It is long plank-shaped or cylindrical, 10-20 cm long, and uneven in thickness. Both the outer and inner surfaces are reddish-brown, and the quality is well broken. The cut side is reddish-brown and rich in oil. It has a distinctive aroma and the taste is spicy and sweet. The pharmacological action of shiitake is known to be good for heart weakness as it promotes blood circulation as a strong cardiac agent, and is good for excitability, stomach upset, warm-heart nourishment, overall meridian veins, yangyang, thermoregulation, improvement of weak constitution, and cold wind.

The term "three-baekcho" used in the present invention has a scientific name of Saururus chinensis (Lour.) Baill. It is also called Cheonseongcho or Sumokgong. It is a perennial plant belonging to the family Trifoliumaceae, mainly growing near water and growing on Jeju Island in Korea. It is known to be effective in preventing and treating various adult diseases such as diabetes, liver disease, various cancers, heart disease, and kidney disease.

Patent Publication No. 10-2010-0130871, Patent No. 10-1621447, Patent Publication No. 10-2015-0036880, Patent No. 10-2040763, Patent No. 10-1461098

It is an object of the present application to provide a complex composition of gondre extract and ginseng extract, gondre extract and rhododendron extract, and rhododendron ginseng extract and anti-obesity use thereof.

The above object of the present application is to prepare a complex composition obtained by mixing each single extract of Gondree extract, Hojang-geun extract, and ginseng extract at a constant mixing ratio; evaluating the cytotoxicity by checking the viability of the single extract obtained in the above step and the complex extract obtained by mixing each single extract at a constant mixing ratio for the 3T3-L1 cell line; measuring the fat accumulation inhibitory effect through Oil-Red-O staining in the above step; In addition, following the above step, the 3T3-L1 cell line of a single extract and complex composition of each natural product was evaluated through the step of measuring the triglyceride production inhibitory effect in comparison with the positive control group.

In the present application, compared to a single material, the complex composition of the extract of rhododendron and ginseng extract has an excellent effect in increasing the cell viability and reducing body fat, as well as having a significantly superior effect compared to the HCA high-dose treatment group.

1 is a graph of the cytotoxicity evaluation result of a single extract of gondre, hojanggeun and trifolieae, and a complex composition mixed at a predetermined mixing ratio;
2 is a graph showing the results of the Oil-Red-O staining experiment to confirm the fat accumulation inhibitory effect according to the experimental plan;
3 is a graph of the experimental results confirming the inhibition of triglyceride production according to the experimental plan;
4 is a photograph showing the fat accumulation or neutral lipid generation effect according to the experimental plan.

The present invention confirmed the cell survival effect on the 3T3-L1 cell line of each single or a combination of each of the extracts of Gondree extract, Hojang-geun extract and Trifolium trellis extract at a constant ratio.

Preparation of extracts of natural products and preparation of complex compositions thereof

The single extract of Gondae, rhododendron and trifolieae prepared in the present invention is hot water or an ethanol extract, more preferably a 30% aqueous ethanol solution.

Their complex composition is a lyophilized product of 9:1 to 1:9 (w/w) mixture of each single extract.

Experimental plan to confirm the prevention and treatment and improvement of obesity

The single extract prepared according to Example 1, each mixed composition at a mixing ratio (w/w) of 2:1 to 3:1 was applied to the experimental plan.

<Experimental Example 1>

Cell survival test (cytotoxicity evaluation)

Cell viability was confirmed for the 3T3-L1 cell line of a single or a mixture of Gondree extract, Hojanggeun extract, and Sambaekcho extract at a predetermined ratio.

As a result of the experiment, the extract of ginseng extract showed a very low (62~65%) cell viability at all concentrations, the extract of Gondree showed about 100% at all concentrations, and the extract of H. japonica was concentration-dependently (50 ug) depending on the concentration of treatment. /mL: about 85%, 100 ug/mL: 76%, 200 ug/mL: about 71%) cell viability.

In addition, the cell viability of the Gondree + Trifolium Root extract complex tends to increase as the composition ratio of Gondae increases, but there is no significant difference. In the Gondree + Trifolium Root extract complex, the cell viability tends to increase as the composition ratio of Gondae increases. There was a clear tendency towards dependence.

Finally, the Gondre + Trifolium Root extract complex showed a cell viability of around 80% at a low concentration (50 ug/mL) of all composition ratios, and a tendency to decrease the cell viability as it increased in a concentration-dependent manner was evident (FIG. 1).

<Experimental Example 2>

Confirmation of fat accumulation inhibitory effect (Oil-Red-O staining)

As a result of the experiment, although the concentration-dependent tendency was insignificant in the case of the monotone extract, it showed similar or high efficiency to that of the Garcinia cambogia extract (HCA), a positive control, even at a low concentration (50 ug/mL).

In particular, in the case of the extract of rhododendron + ginseng extract, the effect is shown in a concentration-dependent manner when the composition ratio of rhododendron + ginseng is 3:1 (w/w), especially at low concentrations (50 ug/mL, 62%) as a positive control group. It can be seen that the phosphorus HCA (79%) showed a higher effect (Fig. 2)

<Experimental Example 3>

Confirmation of inhibition of triglyceride production

In order to confirm the effect on the generation of triglycerides, the 3T3-L1 cell line induced to differentiate was treated with each extract and complex, and then fat-soluble components were extracted from the cells and the triglycerides contained therein were measured using a kit. .

As a result of the experiment, in the case of the positive control group (HCA), about 39.0% of triglyceride was measured compared to the control at 200 ug/mL, and the triglyceride level was lower than that at 200 and 100 ug/mL in the case of a single composition of rhododendron extract.

As mentioned above, in the case of H. janggeun + Trifolium Root extract complex, the neutral lipid production inhibitory effect was shown in a concentration-dependent manner at a composition ratio of 3:1 (w/w), and especially at a low concentration (50 ug/mL), it was higher than HCA 200 ug/mL. It was confirmed that a high effect was exhibited (FIG. 3).

<Experimental Example 4>

Confirm the effect of reducing body fat

In the case of a single material according to the present invention, although rhododendron and trifolieae showed a low cell viability, the body fat reduction effect showed a clear tendency in a concentration-dependent manner, and in the case of the gondre extract, the cell viability was very high, but the fat accumulation or triglyceride production was inhibited. The effect was found to be negligible.

However, the cell viability of the complex composition of rhododendron + trifolieae showed a tendency to slightly increase compared to each single composition. In particular, the effect of reducing body fat was confirmed even at low concentrations in both the Oil-Red O staining method and the TG analysis method. Since a significantly higher effect was confirmed compared to the HCA high-dose treatment group used as a positive control in the experiment, it was determined to be the most suitable composite composition in the present invention (FIG. 4).

The present application is a very useful invention in the food and biopharmaceutical industry because it has an excellent effect of providing a food composition for prevention and improvement of obesity containing a complex composition of extracts of natural substances raw material Hojang-geun and ginseng extract.

Claims (3)

A complex composition containing a mixture of hojang-geun and ginseng extract. A functional food composition for preventing and improving obesity comprising the complex composition of claim 1 as an active ingredient. A pharmaceutical composition for preventing and treating obesity comprising the complex composition of claim 1 as an active ingredient.

Images