KR101402936B1 - Compositon with anti-obesity activity comprising extract of bamboo leaf and scutellaria and use thereof - Google Patents

Abstract

The present invention relates to a composition for prevention or treatment of obesity including a bamboo leaf extract and a gold extract, and a functional food for prevention or improvement of obesity including a bamboo leaf extract and a gold extract. The composition of the present invention can be usefully used for obesity and fatty liver improvement or treatment by inhibiting weight loss, reduction of fat tissue weight, and deposition of fat globules in liver tissue.

Description

Compositions with anti-obesity activity comprising extracts of bamboo leaves and golden extracts,

The present invention relates to a composition exhibiting an obesity-suppressing activity comprising bamboo-leaf extract and a gold extract, and a use thereof. More specifically, the present invention relates to a composition for preventing or treating obesity including bamboo leaf extract and gold extract, A functional food for preventing or improving obesity, and a functional food for prevention or improvement of fatty liver.

Due to the recent abundance of diverse and varied diet and lifestyle changes, nutritional intake is likely to be in an unbalanced state, and mechanized modern life has resulted in lack of exercise. As a result, the morbidity of chronic diseases such as hypertension is increasing, and the form of disease is changing to the advanced country type. Studies related to various factors as the cause of these diseases are being actively carried out, and studies on obesity are being actively carried out.

Obesity is a cause of hypertension, diabetes, cardiovascular diseases and various cancers. The medical expenses for the treatment of obesity related diseases are increasing day by day, Has become a big concern. In addition, the methods of treating and preventing obesity are being actively studied in various countries. Currently, dietary treatments that reduce the energy consumption by suppressing the intake of food by currently practiced methods, exercise therapy that emits energy through exercise , Surgery to remove parts of organs or liposuction, and drug therapy using energy metabolism accelerators, appetite suppressants, digestion inhibitors, and the like.

However, until now, there is no complete method of treating obesity, and there are various side effects such as a yoyo phenomenon in which the weight is increased again when the method is stopped, nutritional imbalance due to dietary restriction, and infection due to immunity degradation. Especially in the case of drug therapy, various side effects such as depression, insomnia, digestive disorder and the like are reported. Thus, in addition to these traditional methods, research continues to develop effective and safe methods to promote weight loss.

For example, Korean Patent Registration No. 951414 discloses a method for producing a diet food through drying, crushing, fermentation, extraction, and purification of persimmon leaves, bamboo leaves, golden leaves, green leaves, dermis, marshmallow and Garcinia cambogonia have. However, since the method includes a step of drying, crushing, fermenting, extracting and drying each natural product and then mixing the dried components, there is a disadvantage that the manufacturing process is too complicated and the production unit cost is increased.

Under these circumstances, the inventors of the present invention have made intensive efforts to develop a therapeutic agent derived from a natural product that exhibits safety while exhibiting an excellent activity to inhibit obesity and can be economically produced. As a result, it has been found that a composition comprising bamboo shoot extract and gold extract is safe, The present invention has been accomplished on the basis of the fact that the production process is simple and economical, while exhibiting the effect of inhibiting obesity.

It is an object of the present invention to provide a composition for preventing or treating obesity comprising bamboo leaf extract and gold extract.

Another object of the present invention is to provide a composition for prevention or treatment of fatty liver comprising bamboo leaf extract and gold extract.

Another object of the present invention is to provide a functional food for prevention or improvement of obesity including bamboo leaf extract and gold extract.

It is still another object of the present invention to provide a functional food for prevention or improvement of fatty liver including bamboo leaf extract and gold extract.

In one embodiment, the present invention provides a pharmaceutical composition for preventing or treating obesity or fatty liver including bamboo leaf extract and gold extract.

The term "extract" as used in the present invention means a specific component extracted using a solvent of liquid from bamboo leaves and gold. As the solvent, water, C1-4 alcohol, or a mixture thereof may be used. It is preferable to extract methanol, ethanol or a mixture thereof with water in order to efficiently extract the active ingredient. The golden extract of the present invention may be obtained by extracting roots of gold. As the extraction method, any one selected from the group consisting of hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction and steam extraction can be used.

The term "kelp leaf" of the present invention means a leaf of a bamboo branch, a bamboo branch, a bamboo branch, a bamboo branch, a bamboo branch and the like. In one branch, the efficacy of removing heat, eliminating fussiness, ≪ / RTI >

The term "golden (黄 芩)" of the present invention refers to a perennial plant with a monocotyledonous plant which grows in the mountainous region. Leaves are opposite to each other and have a straight edge. In oriental medicine, roots have been used as antipyretic, diuretic, antipyretic, sedative and anti-inflammatory drugs.

The term "obesity" of the present invention is generally referred to as " obesity " when an excess amount of adipose tissue is present in the body or when the body mass index (body mass index, body weight index divided by the square of height . In individuals with obesity symptoms, the fatty acids and glucose that enter the adipocytes from plasma are usually esterified and accumulate in the form of triglycerides.

The term " fatty liver " of the present invention means a symptom in which more fat is accumulated in the liver than the ratio (5%) of fat in normal liver. The fatty liver can be divided into alcoholic fatty liver due to excessive drinking water, non-alcoholic fatty liver due to obesity, diabetes, hyperlipemia, drug, etc. Alcoholic fatty liver promotes fat synthesis and promotes normal energy metabolism in liver Non-alcoholic fatty liver disease is caused by causes other than alcohol, and it is known that non-alcoholic fatty liver is frequently accompanied by adult disease causing abnormal fat metabolism.

The term "prophylactic " as used in the present invention means any action that inhibits or delays obesity by administration of the pharmaceutical composition. The term "treatment" used in the present invention means all the actions of improving the symptom of obesity by administration of the pharmaceutical composition.

The composition of the present invention includes a bamboo leaf extract and a gold extract. The ratio of the bamboo leaf extract to the gold extract is not particularly limited, but is preferably 1: 0.1 to 1:10 (w / w), more preferably 1: 0.3 to 1: 1 (w / w), most preferably 1: 0.5 (w / w).

According to one embodiment of the present invention, each of the extracts was obtained by immersing and extracting the leaves and golden roots of 80% ethanol, and then mixing the extracts at a constant weight ratio to prepare a composite extract of bamboo leaf and gold Example 1). As a result of verifying the effect of the above-described extract, it was confirmed that not only the weight gain was reduced (Table 1), but also the total body weight was reduced (FIG. 1) and the fat tissue level and fat cell size were also lowered And FIG. 4), it has been confirmed that it reduces the level of adipose tissue and the fat cell size, so that it can be used for effective prevention or treatment of obesity, and also inhibits deposition of fat globules in liver tissues, .

Therefore, the pharmaceutical composition of the present invention can be used for suppressing obesity and fatty liver symptoms by the possibility of obesity and fatty liver symptoms or by administration to an individual who has developed it.

The term "individual" of the present invention means all animals, including humans, who have developed or are capable of developing obesity symptoms.

At this time, the administration route of the pharmaceutical composition can be administered through any general route as long as it can reach the target tissue. The pharmaceutical composition of the present invention may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, orally, intraperitoneally, intrapulmonarily, rectally, but not limited to, as desired . In addition, the pharmaceutical composition may be administered by any device capable of moving the active substance into the target cell.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable diluent, excipient or carrier. The composition comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablet pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose ), Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical composition may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations and suppositories It can have one formulation.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount " of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment And the effective dose level refers to the type and severity of the disease, age, sex, drug activity, drug sensitivity, time of administration, route of administration and rate of release, duration of treatment, Can be determined according to well-known factors. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and administer an amount that will achieve the maximum effect in the least amount without side effects.

As another embodiment for achieving the above object, the present invention provides a health functional food for prevention or improvement of obesity or fatty liver comprising the composition.

The health functional food may be prepared by adding the composition of the present invention to food for the purpose of prevention or improvement of obesity or fatty liver. The health functional food is not particularly limited, but may be a health functional food, a nutritional supplement, And may be any type of food such as food, pharmafood, health food, nutraceutical, designer food, food additive and the like, preferably meat, sausage, bread, chocolate, candy, snack, Noodles, other noodles, gums, dairy products including ice cream, various soups, drinks, tea, drinks, alcoholic beverages, and vitamin complexes. At this time, the method of adding is appropriately used according to a conventional method, and the amount to be added can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

The health functional food of the present invention may further include various components other than the above-mentioned composition. These various components are not particularly limited, but preferably include various nutrients, vitamins, electrolytes, flavors, colorants, Alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, they may contain flesh for the production of natural fruit juice, fruit juice beverage and vegetable beverages to add palatability and / or functionality, and these ingredients may be used independently or in combination.

Since the composition of the present invention shows effects of weight loss and fat tissue weight reduction, it can be widely used for the development of a pharmaceutical composition or health functional food.

FIG. 1 is a graph showing the weight loss level according to the test substance in a high fat diet mouse model. FIG.
2 is a graph showing the level of adipose tissue according to the test substance in the high fat diet mouse model.
3 is a photomicrograph showing the effect of a high fat diet on liver tissue morphology according to the test substance in an obesity mouse model.
4 is a photomicrograph showing the effect of the high fat diet on fat tissue morphology according to the test substance in the obese mouse model.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1: Manufacture of complex extract of bamboo leaf and gold

1 kg of bamboo leaves and 1 kg of golden roots were immersed in 15 liters of 80% ethanol for 1 week at room temperature and extracted and filtered at 80 ° C for 5 hours. Each extract solution was concentrated under reduced pressure to obtain 89.1 g of bamboo leaf extract (BL) and 193 g of a golden extract (SB). The bamboo extract and gold extract were mixed in a ratio of 2: 1 (w / w) (BLSB) was prepared.

Example  2: High-fat diet of bamboo leaf and golden compound extract In the model  Confirming anti-obesity effect

The high fat diet-induced obese mouse model was used to observe the anti-obesity effect of bamboo leaves and golden compound extracts. The results were as follows: normal control (ND), obesity control (HFD), and positive control (Omega-3) groups. For this purpose, a high fat diet - induced obesity mouse model was prepared as follows.

First, seven-week-old male C57bl / 6 mice (Jackson Lab. USA) were exposed to constant temperature (25 ± 2 ° C), humidity (50 ± 5% (# D12492 60 kcal fat, Research Diets Inc., USA) was administered from 8 weeks of age after being ad libitum fed with 1 week of basic diet (AIN-76A diet) From 10 weeks of age, high - fat diet and test substance were administered for 6 weeks.

Next, the test substance (bamboo extract, golden extract or combined extract of bamboo leaf and gold) was administered at a dose of 200 mg / kg / day in two divided doses per day. Dietary intake and body weight were measured and recorded at regular times once a week. The fed test animals were sacrificed and adipose tissues from the abdominal, epididymal and inguinal tissues were obtained from each experimental animal. The weight of adipose tissue per unit body weight (kg) was calculated by measuring the total weight of adipose tissues (Figs. 1 and 2 and Table 1).

In addition, in order to observe pathologic morphology of hepatic and epididymal white adipose cells, the liver tissues and part of epididymal white adipose tissue were anesthetized with ethyl ether after autopsy and fixed in 10% formaldehyde solution for 24 hours, ≪ / RTI > Then, the cells were dehydrated with 2-fold ethanol and embedded in paraffin. The cells were stained with hematoxylin-eosin (HE) stained to a thickness of 4 μm and then subjected to microscopic examination under the light microscope. (Fig. 3). The adipocyte size was measured in 50 μm units using Nikon ACT-1 version 2.51 (Nikon, Japan) (FIG. 4).

Comparison of dietary efficiency according to test substance in high fat diet mouse model Experimental group Feed intake (g / day) Weight gain
(g / day) Dietary efficiency
(FER,%) The normal control (ND)
Obesity Control (HFD)
Positive control (Omega-3)
Bamboo shoot extract (BL)
Combined extract of bamboo leaf and gold (BLSB)
Golden extract (SB) 3.33
2.88
2.57
2.72
2.28
3.01 0.171 + - 0.01
0.483 + 0.03
0.303 + 0.03
0.309 ± 0.05
0.203 + 0.03
0.251 + 0.02 5.15 + 0.36
16.77 ± 1.06
11.78 ± 1.10
11.34 ± 1.96
8.90 ± 1.15
8.35 ± 0.57

As shown in Table 1, in the case of weight gain per day, the obesity control group was increased by about 2.8 times as compared with the normal control group. In the positive control group, the increase in body weight was suppressed by about 1.8 times as compared with the normal control group, The extract was inhibited in weight gain to a level similar to that of the positive control, and the combined extract of gold extract or bamboo shoot and gold was found to be superior to the positive control to inhibit weight gain.

In particular, the combined extract of bamboo leaf and gold increased the daily weight gain by about 1.2 times compared with that of the normal control. Therefore, synergy was induced compared with 1.5 times of gold alone and 1.8 times of bamboo leaf, and induction of weight gain by high fat diet It can be effectively suppressed.

From the viewpoint of dietary efficiency, the obesity control group was increased about 3.3 times as compared with the normal control group. In the positive control group, the dietary efficiency was decreased by about 2.3 times as compared with the normal control group. The dietary efficiency was reduced to a similar level and the combined extract of the golden extract or bamboo shoot and gold was found to be superior to the positive control.

Especially, the combined extract of bamboo leaves and gold increased the dietary efficiency about 1.6 times as compared with the normal control group, and it was found that the induction of weight gain by the high fat diet can be effectively suppressed.

Meanwhile, FIG. 1 is a graph showing a weight loss level according to a test substance in a high fat diet mouse model. As shown in FIG. 1, when the bamboo extract, golden extract, bamboo leaf and gold complex extract were treated, the weight gain was suppressed to the level of the omega-3 group used as a positive control. In particular, the treatment of the combined extract of bamboo leaf and gold showed that the weight gain was significantly suppressed to a level of significance (p <0.05) as compared with the bamboo leaf or gold single extract.

2 is a graph showing the level of adipose tissue according to the test substance in the high fat diet mouse model. As shown in FIG. 2, the weight of the adipose tissue of the normal control group (ND) was extremely low, 0.787 g, and the weight of the adipose tissue was 3.104 g in the obesity control group (HFD) Omega-3), 2.420 g, and 2.640 g of bamboo shoot extract (BL) and 1.910 g (BLSB), respectively. Particularly, in the case of mixed extract of bamboo leaf and gold, the level of fat tissue was significantly (p <0.05) lower than that of bamboo leaf or gold single extract.

3 is a photomicrograph showing the effect of a high fat diet on liver tissue morphology according to the test substance in an obesity mouse model. In the hepatic tissue induced by the high fat diet, the micro lipids were accumulated in the hepatocytes and the micro lipids progressed to macrovesicular lipids, resulting in large fat droplets in the tissues, and hematoxylin- When stained with eosin (HE), fatty accumulation occurs and fat cells with white fat accumulating cells are widely distributed. As shown in FIG. 3, in comparison with the control group (ND), the high fat diet in which obesity was induced by the high fat diet was strongly present in the control group (HFD) and the macrovesicular ) Were observed. (BL), gold extract group (SB), and bamboo leaves and gold complex extract group (BLSB) were similar to or higher than the positive control group (Omega-3) . Compared with the level of white foliage accumulation cells and giant bacillary alopecia in the group treated with extracts of bamboo leaves or gold alone, the bamboo shoots and golden complex extract administered group (BLSB) showed white fat accumulation cells and giant bacilli Normal) was improved in the fatty liver tissue. Therefore, the extracts of Seokbyeol and Gangbyeon were especially effective in inhibiting the accumulation of white fat accumulation cells and giant follicular fat globules in liver tissues and improving fatty liver.

4 is a photomicrograph showing the effect of the high fat diet on fat tissue morphology according to the test substance in the obese mouse model. When the high fat diet is induced by obesity, the size of fat cells is significantly increased. As shown in FIG. 4, the adipocyte size of the control group (HFD) was 4 times higher than that of the normal group (ND), whereas that of the control group (Omega-3) , And the size of adipocytes was decreased to almost normal level in BLSB group. On the other hand, the golden extract administration group (SB) showed almost no effect on the high fat diet similar to the control group (HFD). Therefore, it was confirmed that the extracts of Seokbyeol and Gangbyeon complexes inhibit the increase of adipocyte size with synergistic effect in comparison with each single extract, and thus it can be used for the treatment of obesity induced by high fat diet.

As a result of the above results, it was confirmed that the combined extract of bamboo leaf and gold of the present invention not only reduced the weight gain but also decreased the overall body weight, decreased the fat tissue level and the fat cell size, It can be used for the treatment, and it is also confirmed that it is effective for the improvement of fatty liver by inhibiting deposition of fat globules in liver tissue.

Claims (8)

A pharmaceutical composition for prevention or treatment of obesity comprising bamboo shoot extract and golden root extract.
A pharmaceutical composition for prevention or treatment of fatty liver comprising bamboo leaf extract and golden root extract.
The method of claim 1 or claim 2, wherein the extract and jukyeop golden root extract were maengjongjuk, Timber Bamboo, the water leaves and roots of bamboo gold selected from the group consisting of somdae and Sasa, C _{1 -4} alcohol, or their &Lt; / RTI &gt; by weight of the composition.
3. The pharmaceutical composition according to claim 1 or 2, wherein the ratio of the bamboo leaf extract to the golden root extract is 1: 0.1 to 1:10 (w / w).
A health functional food for prevention or improvement of obesity including bamboo leaf extract and golden root extract.
A health functional food for prevention or improvement of fatty liver including bamboo leaf extract and golden root extract.
The method of claim 5 or 6, wherein the bamboo leaves and the golden root extract are selected from the group consisting of water, C _{1 -4} alcohol, &Lt; / RTI &gt; by weight of the composition.
The health functional food according to claim 5 or 6, wherein the ratio of the bamboo leaf extract to the golden root extract is 1: 0.1 to 1:10 (w / w).

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