KR101903983B1 - Composition for skin whitening comprising Geomgangsong extract as effective component - Google Patents

Abstract

The present invention relates to a skin whitening composition comprising an extract of Ganghwa extract as an active ingredient. The extract of Ganghang extract using the dimeric by-product of the present invention is characterized in that an insoluble fiber component is solubilized by an enzyme treatment and an ultrahigh pressure homogenization treatment to obtain an extraction yield and a physiological activity The composition for skin whitening using the extract of Kumganggang extract effectively inhibits the production of melanin. Therefore, functional cosmetics for skin whitening and skin cosmetics for skin whitening can be effectively used. Can be usefully used as a health functional food or a skin pigmentation disease which can prevent or improve skin pigmentation disease.

Description

[0001] The present invention relates to a composition for skin whitening,

The present invention relates to a composition for whitening skin, which comprises a ginseng extract as an active ingredient.

The skin may be exposed to various harmful substances such as air and ultraviolet ray caused by air pollution and may cause various skin troubles. Among them, dandruff, freckles, and dandruff are among the biggest skin problems, and many efforts have been made to improve them.

Several factors are involved in determining the skin color of a person. Among them, the activity of melanocyte which makes melanin pigment, distribution of blood vessels, thickness of skin, carotenoid, bilirubin and other factors related to pigment are important. In particular, the most important factor is melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. Skin pigmentation is caused by stimuli such as ultraviolet rays or inflammation, and it is also known to be caused by factors such as genetic factors, hormones, foods, and chemicals. In particular, inflammation related factors such as prostaglandins, histamine, alpha-MSH, and NO (nitric oxide) have been implicated in skin pigmentation abnormalities such as spots, freckles and pigmentation, and melanocytes ) Is known to activate melanin production. It is also reported that hyperpigmentation is accompanied by immunological mediators in tissue damage and regeneration process. When melanocytes are stimulated by various factors, tyrosinase produces melanin in the melanosome. As a result, melanocytes are melanosomes. That is, tyrosine in melanocytes is transformed into dopa by the action of tyrosinase and then transformed into dopaquinone by the action of dopaoxidase, and then tyrosinase-related related protein-2 (hereinafter referred to as TRP-2) and tyrosinase-related protein-2 (hereinafter referred to as TRP-2) to produce melanin. In this series of reactions, tyrosinase activates the rate-limiting reaction of the melanin pigmentation process, so that melanogenesis is primarily controlled by the activity and expression of tyrosinase. TRP-2, also known as the DOPA chromium mutant enzyme, activates the reaction of DOPA chromium with 5,6-dihydroxyindole-2-carboxylic acid (DHICA), TRP-1 activates DHICA with carboxylindole- . As a result, TRP-1 and TRP-2 are involved in the polymerization reaction, which is the final step in the formation of the insoluble pigment melanin. It has been reported that tyrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2) are activated by inflammatory mediators to increase the synthesis of melanin .

Currently developed whitening agents include kojic acid, arbutin, glutathione and vitamin C, but consumers do not have a satisfactory whitening effect and their use is limited due to skin safety. Thus, various attempts have been made to synthesize melanin.

The most common method for producing extracts from pine trees is by hot water extraction. Pine needles are poured into water, and boiled or pine needles are boiled in water, or the liquid thus prepared is powder-dried and the dried material is mixed with water again. However, in the extract obtained through conventional methods, most of the solids are precipitated and water is added during the manufacturing process, the purity of the extract is poor, the content of useful components in the produced extract is low, and the extraction process itself is relatively troublesome There were disadvantages.

In order to improve the efficiency and extraction yield of the materials reported so far, it is necessary to improve the yield according to the increase of the surface area according to the fine grinding in the physical aspect, to utilize the ultrasonic wave and the homogenizer for cell disruption and to increase the solubility according to the polarity of the solvent In the biological aspects, fermentation and enzymatic treatment processes for solubilization of insoluble materials have been reported, but the ability of the material to improve the yield and yield has been relatively limited. Recently, the improvement effect of extraction yield through hydrostatic hydrostatic pressure treatment is impossible to apply to mass production by batch type process, and it shows limit to improvement of extraction yield.

Ultra high pressure homogenization technology is a technique of passing a dispersion solution (hydrocolloid) through a micro-orifice module under ultra-high pressure (100 MPa or more) pressure, resulting in cavitation and high shear It is a technique to convert a dispersion solution into a homogeneous phase by deagglomeration and disconnection (depolymerization, low molecular weight) of the dispersions in the dispersion solution with a sudden increase in physical energy. Particularly, it is known that the fiber is made into a homogeneous dispersed phase and is effective for low molecular weight of protein and non-aggregated carbohydrate polymer.

Industrial applications using enzymes have been applied in various industrial fields, and in particular, in the field of food industry, it has become common to use pectinolytic enzymes to inhibit the formation of precipitates in fruit juice processing. However, many attempts have been made to improve the extraction yield and low molecular weight of insoluble components such as cellulose in the extraction process for the materialization, but it is not generalized. In particular, the fiber of the plant has a complex structure of fibrous structure, which is not easy to hydrolyse through a single enzyme treatment, because many fibrous tissues often form a complex with lignin in the fibrous tissue. However, with the help of various nano crushing technology, ultrahigh pressure homogenization technique and nano plasma treatment technology recently introduced, research for solubilizing insoluble polymer materials including cellulose by low molecular weight has been continuously carried out.

On the other hand, pine trees and pine needles have been known to have antimicrobial, sterilizing and deodorizing functions from the past, and Kumkansong, which is also called "Chungyangmok", is known to have excellent deodorizing action due to its unique wood structure. As an example of a technique relating to cosmetics, health functional foods or pharmaceutical compositions containing pine as an active ingredient, the natural fermented pine leaf extract disclosed in Korean Patent Publication No. 2015-0086922 has a composition for relieving skin irritation and skin moisturizing, Patent No. 2016-0038228 discloses a skin whitening composition containing an extract of pine cones as an active ingredient and Korean Patent No. 1608258 discloses a cosmetic composition for moisturizing skin containing a rosin extract, However, no description has been made on a composition for skin whitening comprising the extract of Ganghwa Sangsang as an active ingredient of the present invention.

The present invention provides a skin whitening composition comprising a ginseng extract as an active ingredient, wherein the ginseng extract, which is an effective ingredient, is obtained by enzymatic treatment from thinning by-products (leaves and branches) The present inventors have completed the present invention by confirming that it is possible to effectively inhibit the production of melanin as well as the extraction yield by producing the product through an ultra-high pressure homogenization process.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising an extract of Kumgangsanggang produced by a method comprising the step of adding an enzyme to a mixture of water-softening powder and water and hydrolyzing the mixture, to provide.

In addition, the present invention provides a pharmaceutical composition for preventing or treating melanin pigment hyperpigmentation diseases, which comprises the above extract of King Sejong transportation as an active ingredient.

The present invention also provides a health functional food composition for preventing or ameliorating hyperpigmentation of melanin pigment comprising a fermented product as a active ingredient.

The extract of Kum-jang Song using the thinning by-products of the present invention is obtained by solubilizing insoluble fibrous components through enzyme treatment and ultrahigh-pressure homogenization treatment to improve the extraction yield and physiological activity, and can efficiently utilize the discarded by- And the composition for skin whitening using the extracts of the present invention effectively inhibits the production of melanin. Therefore, functional cosmetics for skin whitening and health functional foods capable of preventing or improving skin pigmentation diseases or skin pigmentation diseases And the like.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing melanin biosynthesis of an extract of King Sejong Institute of the present invention. FIG.

In order to achieve the object of the present invention,

There is provided a cosmetic composition for skin whitening comprising, as an active ingredient, a gingko extract prepared by a method comprising the steps of:

(a) drying and grinding the soft steel sheet to produce a soft steel powder;

(b) hydrolyzing the mixture obtained by adding water to the monosilane powder prepared in the step (a) after adding the enzyme;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the ethanol-treated hydrolyzate of step (c) by ultra-high pressure.

In the method for producing the extract of the invention, which is an active ingredient of the cosmetic composition for skin whitening of the present invention, the step (a) of the step (a) may preferably be a leaf and a branch of a pearling, more preferably, And can be used as a raw material of Kumgangsong (a by-product of Kumgang Song) collected from leaves and branches of Kumgangsong.

In the method of the present invention, the enzyme of step (b) may be Viscozyme. Viscozyme is a commercially available enzyme, and is an Arabidase derived from Aspergillus aculeatus It is a complex enzyme including arabinase, cellulase, beta-glucanase, hemicellulase, and xylanase.

According to one embodiment of the present invention, the byproducts of the garnet thinning can be inhibited from the yield and melanin synthesis of the ginseng extract by high pressure homogenization after treatment with viscose.

In step (b) of the method of the present invention, the step (b) is preferably a step of adding viscose (Viscozyme) to a mixture prepared by adding 10 to 14 times (v / ) And then hydrolyzed at 30 to 60 ° C for 1 to 12 hours, more preferably 12 times (v / w) of water adjusted to pH 4.5 in Kumkang Song powder is added to a mixture of Viscozyme ) And then hydrolyzed at 50 ° C for 8 to 12 hours. The hydrolysis of the polystyrene powder under the above conditions can improve the extraction yield by improving the extraction yield and improving the elution of the active material, by insolubilizing the insoluble matter in the gold wire girdle into a low molecular weight or solubilizing it through molecular rearrangement.

In addition, in the method for producing the extract of the invention, the ethanol treatment in the step (c) is preferably carried out by adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate, (V / v) of 100% ethanol is added to the hydrolyzate, and the mixture is stirred at 20 to 30 ° C for 2 hours at 180 rpm.

In addition, in the method for producing the extract of the present invention, the ethanol-treated hydrolyzate may further include a step of centrifuging and recovering the supernatant.

In addition, in the method of the present invention, the ultra-high pressure homogenization of step (d) can be homogenized at a pressure of 100-120 MPa, and more preferably at a pressure of 110 MPa.

The method for producing the extract of the present invention is more preferably

(a) drying and pulverizing leaves and branches of the garment to prepare a paulowniae powder;

(b) Viscozyme is added to the mixture obtained by adding 10 to 14 times (v / w) of water adjusted to a pH of 3.5 to 5.5 to the Kumong propagation powder prepared in the step (a) Hydrolyzing for 1 to 12 hours;

(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And

(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa.

In the present invention, it is possible to improve the extraction yield of the active material by making the fibers in the ammonium sulfate extract into a uniformly dispersed phase by enzyme treatment and ultra-high pressure homogenization treatment, and by lowering the molecular weight and solubilizing the insoluble dietary fiber.

On the other hand, the whitening effect refers to an effect to improve or prevent glaring skin, spots, freckles, and dark circles caused by various factors such as exposure to ultraviolet rays, change in hormone balance, genetic program, Or an effect of maintaining a beautiful skin with a sense of transparency and an effect of reducing the dullness of the skin to increase gloss and tautness. In general, it is known that the skin, spots, freckles, and dark circles of the skin, stamens, freckles and dark circles are stimulated by melanocytes due to stimulation by ultraviolet rays or changes in hormone balance, and melanin pigment is deposited on the skin. Therefore, if the production of melanin can be inhibited, it is possible to prevent or improve gross skin, spots, freckles and dark circles.

The cosmetic composition for skin whitening may be a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, an emulsion foundation, a wax foundation and a spray , But the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, at least one skin whitening active ingredient exhibiting the same or similar functions. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenylalkanol, 2,7-dinitroindazole or Plant extracts such as mandarin orange extract, rice extract, licorice extract, and the like, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

In addition, the present invention provides a pharmaceutical composition for preventing or treating melanin pigment hyperproliferative disorder, which comprises, as an active ingredient, a goldfish extract prepared by a method comprising the steps of:

(a) drying and grinding the soft steel sheet to produce a soft steel powder;

(b) hydrolyzing the mixture obtained by adding water to the monosilane powder prepared in the step (a) after adding the enzyme;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the ethanol-treated hydrolyzate of step (c) by ultra-high pressure.

In the present invention, The method for producing the gimme extract which is the active ingredient of the composition is as described above. The drug? The composition preferably further comprises a carrier, an excipient or a diluent in addition to the gingiva extract, but is not limited thereto.

As used herein, the term " melanin pigment hyperpigmentation "means darkening or darkening compared to other areas due to excessive increase of melanin in specific areas of skin or hands and toenails.

The melanin pigment hyperpigmentation disorder can be selected from the group consisting of freckles, senile spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, gravidic chloasma, Post-inflammatory hyperpigmentation due to burns or dermatitis including burns, and the like.

The pharmaceutical composition of the present invention may be various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of suppositories include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and it is preferable to select the intraperitoneal, rectal, rectal, intravenous, intramuscular, subcutaneous, intrauterine or intracerebral injection methods during parenteral administration, It is preferably used for external skin application.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition of the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The dosage of the pharmaceutical composition of the present invention may be varied depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease.

The present invention also provides a health functional food composition for preventing or ameliorating a melanin pigment hyperproliferative disorder, which comprises, as an active ingredient, a gangjae extract prepared by a method comprising the steps of:

(a) drying and grinding the soft steel sheet to produce a soft steel powder;

(b) hydrolyzing the mixture obtained by adding water to the monosilane powder prepared in the step (a) after adding the enzyme;

(c) treating the hydrolyzate of step (b) with 100% ethanol; And

(d) homogenizing the ethanol-treated hydrolyzate of step (c) by ultra-high pressure.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient can be suitably used depending on its use purpose (prevention or improvement). Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in case of long-term ingestion for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the health functional food. Examples of the foods to which the health functional food composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, soups, Drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, in the case of a drink, a natural carbohydrate or a flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Production Example 1. Preparation of Kumgangsong extract

In order to observe the extract yield and compositional change of the extract according to the type of enzymes and the presence of ultra - high pressure homogenization, extracts were prepared as follows according to enzyme type, presence of ultra - high pressure homogenization and enzyme treatment + hyperbaric homogenization.

(1) Enzyme treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of viscozyme and pectinex (enzymes) were added to 50 g / 100 g of 500 unit / Ml, and the mixture was stirred at 50 DEG C and 37 DEG C at 180 rpm. The enzyme was treated at the optimum time (10 hours and 2 hours), and then 70 ml of 100% ethanol was added. The mixture was extracted at 180 rpm for 2 hours at room temperature and then centrifuged at 12,000 rpm for 15 minutes.

(2) Ultra high pressure homogenization treatment

30 ml of distilled water adjusted to pH 4.5 and 70 ml of 100% ethanol were added to 2.5 g of dried and pulverized gangmonggwon by-products or sawdust samples, and the mixture was stirred and centrifuged at 12,000 rpm for 15 minutes and filtered to prepare a filtrate And then passed through an orifice tube twice at a pressure of 110 MPa.

(3) Enzyme treatment and high pressure homogenization parallel treatment

30 ml of distilled water adjusted to pH 4.5 and 2.5 g of Viscozyme and pectinex (enzymes) were added to 50 g / ml and 500 unit / Lt; 0 > C and 37 [deg.] C at 180 rpm. The enzymes were treated at optimal times (10 hours and 2 hours), and 70 ml of 100% ethanol was added. The mixture was extracted at 180 rpm for 2 hours at room temperature (25 ° C), centrifuged at 12,000 rpm for 15 minutes, And then passed through an orifice tube twice under a pressure of 110 MPa.

Example 1. Confirmation of Extraction Yield of Kumkang Song

Changes in the extraction yield of various enzymes and ultrahigh pressure treatments were measured for functional materials using the extracts of Kumkangsong. As a result, it was found that the extraction yield was improved when the enzyme treatment was performed and that the extraction yield was improved by about 17% when the ultra high pressure homogenization was performed. Especially, when the enzymatic treatment and the ultrahigh pressure homogenization treatment were applied, the yield improvement was better than that of Kumkangwon powder. Especially, when the by - product of thinning was used, the highest yield was obtained by 91% (Table 1). When sawdust was used as a raw material, the yield could not be confirmed because it did not become an ultra-high pressure homogeneous after treatment with viscose.

Extraction Yield (%) of Geumgang Song by Enzymatic Treatment and Ultra High Pressure Homogeneous Treatment Cashmere raw material Treatment conditions of by-products Extraction yield (%) Growth rate compared to untreated group (%) Thinning Untreated group 24.29 Enzyme V (viscozyme) 35.20 45.0 Enzyme P (pectinex) 29.80 23.0 Ultrahigh pressure homogeneity 28.47 17.2 Enzyme V + Ultra High Pressure Homogeneity 46.50 91.4 Enzyme P + ultrahigh pressure homogeneity 31.02 27.7 sawdust Untreated group 4.98 Enzyme V (viscozyme) 7.34 47.0 Enzyme P (pectinex) 8.64 73.0 Enzyme P + ultrahigh pressure homogeneity 8.18 64.2

Example 2. Confirmation of whitening activity of Kumkangsong extract

In order to confirm the whitening activity of Ganghwa extract, the amount of melanin biosynthesis was measured. B16F10 cells were inoculated in 12 wells at a density of 1 × 10 ⁵ cells / ml and cultured. After 24 hours, thallus extract (50, 100, 200 μg / ml) and arbutin (100 μg / ml) Lt; / RTI > for 48 hours. After the treatment, the cells were washed twice with PBS (phosphate buffered saline) and centrifuged to make cell precipitate. 200 μl of 1 N NaOH solution containing 10% DMSO (dimethyl sulfoxide) was added and dissolved at 60 ° C for 1 hour. Absorbance was measured at 405 nm.

In order to measure the amount of melanin biosynthesis in the thinning extracts through enzymatic treatment and homogenization, all of the dilutions were diluted to the same dilution, and all of them were as effective as albutin (Ar) as a whitening agent. It was confirmed that melanin production was lowered in the extract with the high-pressure homogenization in comparison with the untreated group (without the enzyme treatment or the ultra-high pressure extraction) to exhibit more excellent efficacy (FIG. 1).

Claims (7)

A cosmetic composition for skin whitening comprising an extract of Ganghwa extract prepared by a method comprising the steps of:
(a) drying and pulverizing leaves and branches of the garment to prepare a paulowniae powder;
(b) Viscozyme is added to the mixture obtained by adding 10 to 14 times (v / w) of water adjusted to a pH of 3.5 to 5.5 to the fabric of the present invention at 30 to 60 ° C Hydrolyzing for 1 to 12 hours;
(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa. delete delete delete delete A pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, comprising an extract of Ganghwa extract prepared by a method comprising the steps of:
(a) drying and pulverizing leaves and branches of the garment to prepare a paulowniae powder;
(b) Viscozyme is added to the mixture obtained by adding 10 to 14 times (v / w) of water adjusted to a pH of 3.5 to 5.5 to the fabric of the present invention at 30 to 60 ° C Hydrolyzing for 1 to 12 hours;
(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa. A health functional food composition for preventing or ameliorating a melanin pigment hyperpigmentation disease comprising an extract of Ganghwa extract prepared by a method comprising the steps of:
(a) drying and pulverizing leaves and branches of the garment to prepare a paulowniae powder;
(b) Viscozyme is added to the mixture obtained by adding 10 to 14 times (v / w) of water adjusted to a pH of 3.5 to 5.5 to the fabric of the present invention at 30 to 60 ° C Hydrolyzing for 1 to 12 hours;
(c) adding 6 to 8 times (v / v) 100% ethanol to the hydrolyzate of step (b) and treating the mixture at 20 to 30 ° C for 1 to 3 hours; And
(d) homogenizing the ethanol-treated hydrolyzate obtained in step (c) under a pressure of 100 to 120 MPa.

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