KR101739086B1 - Cultivating method of grifola frondosa - Google Patents

Abstract

More particularly, the present invention relates to a method of cultivating leaf mushroom, and more particularly, to a method of cultivating leaf mushroom, and more particularly, to a method of cultivating leaf mushroom, The method comprising the steps of: preparing a plurality of pierced grooves; sterilizing the medium having the pierced holes formed therein through the piercing step; spraying the seedling mushroom seeds into one of the pierced holes formed in the sterilized medium through the sterilization step And a culture step of culturing the culture medium onto which the seeds have been sprayed through the seed spreading step.
Since the cultivation method of the mushroom with the above-mentioned process uses the culture medium formed with a lot of pore grooves, the growth period of the mushroom is fast and the culture period is shortened.

Description

{CULTIVATING METHOD OF GRIFOLA FRONDOSA}

The present invention relates to a method of cultivating a leaf mushroom, and more particularly, to a method of restoring a leaf mushroom which has a short growth period due to a high growth rate of a leaf mushroom mycelium because a culture medium having many pit grooves is used.

Leaf mushroom is a mushroom belonging to the mushroom family, and it grows in the base of the tree in autumn in the nature. Fruiting bodies of leaf mushrooms are complex, large mushroom pods that form a group of numerous small ghats at the ends of branches of a multi-branched sack. The umbrella is semicircular or fan-shaped, 2 to 5 cm wide, 2 to 4 mm thick, and the surface initially becomes black to gray or grayish brown. In addition, the flesh is white and fleshy, and when dried, it is hard and crumbly.

The bottom of the mushroom leaves is white and hangs down on the bag, and the bag hanging is thick and white. Spores are elliptical and smooth, spore patterns are white, and are white rot fungi parasitized on old trees of oak trees.

It has been known that Leaf mushroom having such characteristics is excellent in anticancer activity, blood pressure lowering, diabetes, obesity treatment (diet), reduction of blood cholesterol, antibacterial action, diuretic action, tonic action, The cultivation method has been developed, and it is an edible mushroom with good taste and fragrance, and it is distributed in Korea, Asia, Europe, and the United States.

However, conventionally, the artificial cultivation method of Leaf mushroom has taken about 40 days or longer to cultivate the Leaf mushroom, which causes a problem that productivity is lowered.

Korean Patent Registration No. 10-0879722 (January 14, 2009). Korean Patent Registration No. 10-1283450 (2013.07.02).

It is an object of the present invention to provide a method for restoring leaf mushrooms in which the growth period of leaf mushrooms is fast and the cultivation period is shortened because the medium is formed by using a medium in which many pit grooves are formed.

The object of the present invention is to provide a method for producing a microorganism which comprises a medium mixing step of mixing wheat bran, sawdust and calcium carbonate, a pouring step of putting the medium prepared through the medium mixing step into a culture container, A step of sterilizing a medium in which a pit-hole is formed through a punching step, a step of spraying a seedling mushroom seed to one of pore grooves formed in a sterilized medium through the sterilization step, And a cultivation step of culturing the medium. The present invention also provides a cultivation method of the mushroom.

According to a preferred aspect of the present invention, a step of inserting a perforated groove supporting bar having a ring in one of the perforated grooves formed through the perforating step is inserted between the perforating step and the sterilizing step, A support bar removing step of removing the perforated groove support bar after binding the upper end of the culture container on the basis of the ring provided on the support bar, and a step of removing the support bar from the support bar by removing the support bar, And a sealing step.

According to a more preferred feature of the present invention, the seedling spraying step is performed by spraying the mushroom seedlings after removing the membrane formed on the pore groove through the sealing step.

According to a further preferred feature of the present invention, the medium mixing step is performed by mixing 100 parts by weight of sawdust, 20 to 30 parts by weight of wheat bran, and 2 to 3 parts by weight of calcium carbonate.

According to an even more preferred feature of the present invention, the perforation grooves formed in the medium in the perforating step are 3 to 7.

According to a further preferred feature of the present invention, the sterilization step is performed by heating the culture medium having the pierced hole formed therein through the piercing step at a temperature of 120 to 125 DEG C for 120 to 140 minutes.

According to an even more preferred feature of the present invention, the perforation groove has a diameter of 2 to 3 centimeters and a depth of 10 to 20 centimeters.

According to still another more preferred characteristic of the present invention, the seedling spraying step is performed by spraying 0.05 to 0.1 part by weight of seedling mushroom seeds on 100 parts by weight of the sterilized medium through the sterilization step.

Since the cultivation method of the leaf mushroom according to the present invention is carried out using a culture medium formed with a plurality of pit grooves, the growth period of the leaf mushroom is fast and the culture period is shortened.

1 is a flowchart illustrating a cultivation method of a mushroom according to an embodiment of the present invention.
2 is a flowchart illustrating a cultivation method of a mushroom according to another embodiment of the present invention.
3 is a perspective view schematically showing a mushroom punch automatic piercing apparatus according to an exemplary embodiment of the present invention.
4 is a perspective view schematically illustrating a hopper of a mushroom punch automatic piercing apparatus according to an exemplary embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

The method for cultivating the mushroom according to the present invention comprises: a medium mixing step (S101) for mixing wheat bran, sawdust and calcium carbonate; feeding the medium prepared through the medium mixing step (S101) into a culture vessel; (S103) forming a plurality of pierced holes, sterilizing a medium having pore holes formed through the piercing step (S103) (S105), forming a piercing hole formed in the sterilized medium through the sterilizing step (S105) (S107) for spraying the mushroom seedlings on one of the seeds (S107) and a seeding step (S109) for culturing the medium onto which the seeds have been sprayed through the seeding spraying step (S107).

The medium mixing step (S101) comprises mixing 100 parts by weight of sawdust, 20 to 30 parts by weight of wheat bran, and 2 to 3 parts by weight of calcium carbonate by mixing bran, sawdust and calcium carbonate.

In the medium mixing step (S101), sawdust which occupies the largest proportion among the materials constituting the culture medium can be selected from oak sawdust or oak chips, and the oak sawdust and oak chips are mixed in a weight ratio of 1: 1 The medium composed of the above components preferably has a moisture content of 60 to 70%, more preferably 64%.

The purging step (S103) is a step of putting the medium prepared through the medium mixing step (S101) into a culture container and forming a plurality of pierced holes using the piercing device, Into a culture container made of a plastic container or a plastic material, and piercing 3 to 7 pierced holes using a piercing device.

If the number of the perforation grooves is less than 3, the spraying efficiency of the leaf mushroom seedlings is lowered in the seedling spreading step (S107), the incubation period of the leaf mushrooms becomes longer, and when the number of the perforated grooves exceeds 7, The culture period of the mushroom is not greatly improved and the efficiency of the pore groove forming process is lowered.

At this time, the perforation device (1) automatically inserts the mushroom cultivation medium into the envelope and automatically forms the inoculation seeds, separates the step of performing the operation through the step rotation method, A hopper 200, a tread 300, a rotation unit 400, a clamping unit 500, and a control unit 600. The main frame 100, the hopper 200, the treadmill 300, The hopper 200 installed on the main frame 100 includes a stirring member 203 and a rotation driving unit 201 for rotating the stirring member 203. The rotation unit 400 installed in the main frame 100 includes a vertical central axis 401 extending vertically and a central axis rotation driving unit 403 for stepping the central axis 401, A support shaft 405 which is connected to a lower portion of the support shaft 401 and rotates together with the center shaft 401 and rotates together with the center shaft 401 and is spaced apart from the support shaft 405, And an injection plate 407 having a plurality of through holes 409 formed at regular intervals and a through hole guide surface 411 extending downward along the perimeter of the through holes 409. The clamping part 500 installed at one side of the lower part of the injection plate 407 for each of the through holes 409 is supported by the support plate 405 and is for clamping the discharge envelope inserted into the through hole 409. The tread 300 installed on the upper side of the main frame 100 and positioned above the injection plate 407 is provided with a pressure actuator 301 and a lower part connected to the lower part of the pressure actuator, And a rotation driving unit 303 installed between the pressing actuator 301 and the punching rods 307 and 309 for rotating the punching rod. The control unit 600 is for supplying or shutting off electricity to the hopper rotation drive unit 201, the central axis rotation drive unit 403, and the pressure actuator 301.

Referring to FIGS. 3 and 4, the hopper 200 includes a rotation drive unit 201 for the hopper, stirring members 203 and 205, and a hopper 200. The hopper 200 temporarily stores the mushroom medium and supplies a proper amount of mushroom medium to the medium envelope. And includes a discharge port cover 209 and a hopper guide surface 207.

The stirring members 203 and 205 are rotated by the rotation driving unit 201 for the hopper to stir the mushroom culture medium in the hopper 200. Particularly, the screw stirring member 205 located at the lower portion of the hopper 200 and adjacent to the hopper outlet cover 209 has a helical screw shape. When the screw stirring member 205 rotates, The mushroom medium is pushed out to the discharge port cover 209 along the spiral screw of the screw stirring member 205. At this time, the hopper outlet cover 209 is connected to the hopper 200 by the hinge 211 as shown in FIG. 1, so that the hopper 200 automatically rises up due to the pushing force of the mushroom medium. When the screw stirring member 205 rotates The hopper outlet cover 209 descends again to close the outlet. In this manner, the mushroom culture medium can be discharged out of the hopper 200, and after being discharged, into the through hole 409 of the injection plate 407 along the hopper guide surface 207.

Since the seed medium sprayed in the seed spraying step (S107) penetrates into the inside of the medium through the perforation groove formed in the medium, the medium having the perforated groove formed through the perforation apparatus (1) The cultivation speed of the leaf mushroom is greatly improved compared to the cultivation method. In general, the cultivation period of the leaf mushroom is about 40 to 50 days. When the culture medium having pore grooves is used as described above , The bacterium of the leaf mushroom grows rapidly in the medium, and the culture of the leaf mushroom can be completed in about 20 to 25 days.

At this time, it is preferable that the culture container is capable of accommodating 2.5 to 3 kg of the mixed medium through the medium mixing step (S101). In the case of the culture container in which 2.5 to 3 kg of medium is added as described above, , It is superior in efficiency to those cultivated and discarded once.

The sterilization step S105 is a step of sterilizing the medium having the pit-hole formed through the punching step S103. The medium having the pit-hole formed through the punching step S103 is heated at a temperature of 120 to 125 ° C, Min. After the sterilization step (S105), the microorganisms contained in the medium constituting the culture medium are removed, so that the seeds of the mushroom can be rapidly proliferated and the mushroom of excellent commercial quality is provided.

If the heating temperature is less than 120 ° C., the sterilization efficiency is lowered. In addition, the sterilization process takes a long time to lower the workability. If the temperature of the sterilization step (S 105) exceeds 125 ° C. , The nutritional components contained in the materials constituting the medium may be destroyed, and the cultivation efficiency of the leaf macker may be lowered.

If the heating time is less than 120 minutes, the sterilization process of the medium does not progress properly. If the heating time exceeds 140 minutes, the heating is continued in a state where the sterilization is almost completed, and the efficiency of the process is lowered.

(S104-1) for inserting a perforated groove supporting bar having a ring in one of the perforated grooves formed through the perforating step (S103) between the perforating step (S103) and the sterilizing step (S105) (S104-2) for removing the perforated groove support bar after binding the upper end of the culture container with respect to the ring provided in the inserted support bar through the inserting step (S104-1) and the support bar removing step (S104-2 The sealing step S104-3 of sealing the ring formed on the upper end of the culture container with the cotton may be further performed. In the above-described insertion step S104-1 of the support bar and the removal of the support bar (S104-2), the shape of the pierced hole is maintained in the process of binding the upper end of the culture container, so that the seed can be uniformly sprayed into the culture container in the seed spraying step. In the sealing step (S104-3) , The sterilization step (S105) is performed It is possible to inhibit the penetration of the germs into the sterilized culture container.

At this time, the ring provided in the perforated groove supporting bar inserted into the perforated groove through the supporting bar inserting step (S104-1) is removed by removing the perforated groove supporting bar through the supporting bar removing step (S104-2) And is fixed to the upper end of the culture container by rubber strings or the like so as to prevent the pierced hole from being clogged by external force and to maintain the shape of the groove.

The seedling spraying step (S107) is a step of spraying the mushroom seedlings to the sterilized medium through the sterilization step (S105). In the sterilization step (S105), 100 parts by weight of the sterilized medium is sprayed with 0.05 to 0.1 By weight.

At this time, in the seeding step S107, it is preferable that the membrane formed in the pore groove through the sealing step (S104-3) is formed by spraying the mushroom seedlings after the cotton is removed, The infiltration of germs is suppressed in the container, and the mushroom seedlings sprayed through the seeding step (S107) can rapidly grow.

In addition, when the seedling mushroom seedlings are sprayed through the seedling spraying step (S107), the seedlings of the leaf mushroom are rapidly infiltrated by a plurality of pore grooves formed in the sterilized medium through the punching step (S103) , It is possible to shorten the cultivation time of the leaf mushroom.

The culturing step (S109) is a step of culturing the medium onto which the seeds have been sprayed through the seeding step (S107), and the medium to which the seeds have been sprayed through the seeding step (S107) To 40% humidity and carbon dioxide at 2000 to 3000 ppm for 20 to 25 days.

After the above cultivation step (S109), the growth of the leaf mushroom is completed. In the above culture step, since a large amount of oxygen is easily supplied to the medium, the high quality leaf mushroom is cultivated so as to be ready for shipment.

Therefore, since the cultivation method of the mushroom according to the present invention is carried out using a culture medium formed with a plurality of pore grooves, the growth period of the mushroom is fast and the culture period is shortened.

S101; Medium mixing step
S103; Step
S104-1; Step of inserting a support rod
S104-2; Step of removing support rods
S104-3; Sealing step
S105; Sterilization step
S107; Seed spraying step
S109; Incubation step
1: Automatic mushroom badge piercing device
2: Automatic piercing device of mushroom badge
10: Mushroom badge
20: badge envelope
100: Mainframe
200: Hopper
201: Rotary drive unit for the hopper
203: stirring member
205: screw stirring member
207: hopper guide surface
209: Hopper outlet cover
211: Hinge
300:
301: Pressure actuator
303: piercing rod rotation driving part
305: compass plate
307: Central piercing rod
309: Perforated rod
311: Central bored hole rod gear
313: Peripheral perforated rod gear
315: guide rod
400: rotation unit
401: center axis
403:
405:
407: Injection plate
409: Through hole
411: Through hole guide face
500: clamping part
600:

Claims (8)

A medium mixing step of mixing wheat bran, sawdust and calcium carbonate;
A piercing step of putting the culture medium prepared through the medium mixing step into a culture container and forming a plurality of pierced holes using the piercing device;
A sterilizing step of sterilizing the culture medium having the pierced hole formed therein through the piercing step;
A step of spraying the seeds of the mushroom on the one of the pit grooves formed in the sterilized medium through the sterilization step; And
And a culture step of culturing the medium onto which the seeds are sprayed through the seeding step,
A support bar inserting step of inserting a perforated groove supporting bar having a ring in one of the perforated grooves formed through the perforating step between the perforating step and the sterilizing step;
A support bar removing step of removing the piercing hole support bar after binding the upper end of the culture container with respect to the ring provided in the support bar inserted through the support bar insertion step; And
A sealing step of sealing the ring formed on the upper end of the culture container with a cotton wool after the support bar is removed through the removal of the support bar,
In the punching step, the pore grooves formed in the medium are 5 to 7,
Wherein the sterilization step comprises heating the medium having the pit-hole formed therein through the piercing step at a temperature of 120 to 125 DEG C for 120 to 140 minutes.
delete The method according to claim 1,
Wherein the seedling spraying step is performed by spraying the mushroom seedlings after removing the membrane formed on the pore groove through the sealing step.
The method according to claim 1,
Wherein the medium mixing step comprises mixing 100 parts by weight of sawdust, 20 to 30 parts by weight of wheat bran, and 2 to 3 parts by weight of calcium carbonate.
delete delete The method according to claim 1,
Wherein the perforation groove has a diameter of 2 to 3 centimeters and a depth of 10 to 20 centimeters.
The method according to claim 1,
Wherein the spraying step is performed by spraying 0.05 to 0.1 part by weight of a mushroom seedling on 100 parts by weight of the sterilized medium through the sterilization step.

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