KR101650873B1 - Method of cultivating mushrooms using bottle cap - Google Patents

Abstract

The present invention relates to a method of inserting a mushroom using a bottle cap having perforated holes in the edge portion. According to the insecticidal method of the present invention, there is no spoilage due to germs, quality is excellent, Can be provided.

Description

TECHNICAL FIELD The present invention relates to a method of cultivating mushrooms using a bottle cap,

More particularly, the present invention relates to a method of bottling a mushroom using a bottle cap having a perforated hole in the edge portion thereof.

Generally, the method of mushroom mushrooms includes logging, bagging, and mushrooms, and an appropriate method should be selected according to the type of mushroom to be cultivated and the environmental conditions.

Among the methods of treating the mushroom, the mushroom is cultivated at a temperature suitable for growth of the mycelium by inoculating sawdust into the mushroom and sterilizing the mushroom with the steam, sterilizing the mushroom culture, And a fruiting body is generated on the surface of the bundle.

In general, the above method is a method of cultivating the mushroom in a cultivation room without removing the lid and scratching the mushroom after the completion of the cultivation.

However, when cultivated by the general method as described above, not only the contamination during the cultivation process but also the direct influence of the cultivation environment such as ventilation, humidity, temperature, etc. grows differently in each culture bottle (container) There are many difficulties, and most of all, the mushrooms are different from each other and the merchantability is very low.

In addition, in harvesting mushrooms, it is harvested by harvesting or picking mushrooms, or harvested by using tools.

On the other hand, as a prior art relating to the method of inserting mushrooms using a bottle lid, there has been disclosed a method of inserting mushrooms to uniformize the shape of fruiting bodies (Korean Patent Laid-Open No. 10-2010-0122714) As a method of bottling using a perforated bottle cap, the size of the fruiting body is fixed and can be standardized, but it has a disadvantage that the yield is low due to decay by germs and the like.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a mushroom bottle dispenser having a uniform shape by using a bottle cap in a conventional mushroom bottle dispensing method, It is possible to harvest mushrooms having a constant weight and high value of commodity at a high yield, and to provide a method for bottling the rice which is very convenient for harvesting.

Another object of the present invention is to provide a mushroom cultivated by the above method.

In order to achieve the above object, the present invention provides a method for bottling a mushroom using a bottle lid, wherein the bottle lid has a perforated hole at the edge of the bottle lid.

In addition, the present invention provides a mushroom prepared by the above method.

Since the mushroom according to the present invention is cultivated, the mushroom is cultivated and harvested by closing the inlet of the culture bottle by replacing with a new lid perforated after the completion of cultivation. Thus, Since a fruiting body is formed to take a form having a constant shape, there is an advantage in that the merchantability is very excellent.

In addition, since the mushroom bottle according to the present invention uses a bottle cap having a perforated hole in the edge portion, there is no corruption due to germs.

In addition, the method of bottling the mushrooms according to the present invention is advantageous in that the size and weight of the mushrooms are almost constant since the mushrooms are harvested when they grow to the full outer diameter of the top bottle lid.

In addition, since the bottle cover is flat in the mushroom harvesting process according to the present invention, the mushroom can be harvested even by pushing or pulling the bottom portion of the mushroom between the bottle cap and a tool such as a knife Therefore, the harvesting operation is very convenient, and since it does not apply excessive force to harvest the mushroom, there is an advantage that it can be harvested without damaging the quality.

FIG. 1 is a plan view of a bottle cap according to a temporal example used in the method of bottling mushrooms according to the present invention.
FIG. 2 is a perspective view of a bottle lid according to a temporal example used in the method of bottling mushrooms according to the present invention.
FIG. 3 is a perspective view of a mushroom grown on a bottle lid according to a temporal example used in the method for growing mushrooms according to the present invention.
Fig. 4 is a photograph showing the fruit bodies harvested by Example 2, Example 3 and Comparative Example 1. Fig.

The present invention provides a method of bottling a mushroom using a bottle lid, wherein the bottle lid having a perforated hole at the edge of the bottle lid is used.

In accordance with the method of the present invention, the method for preparing a mushroom comprises: preparing a mushroom culture medium and introducing the prepared culture medium into a cultivation bottle; Attaching a high pressure sterilizable tape to a bottle cap having perforated holes in the edge region; Sealing the bottle with the bottle lid to which the tape is attached, and sterilizing the bottle; Inoculating the mushroom seeds into the medium in the sterilized bottle; Culturing the inoculated mushroom seeds; Removing the tape from the bottle cap after completing the seed culture; Placing the cultivation bottle from which the tape has been removed in a cultivation room and fingering the mushroom; And a step of growing the above-mentioned mushroom.

In accordance with the method of the present invention, the method for preparing a mushroom comprises: preparing a mushroom culture medium and introducing the prepared culture medium into a cultivation bottle; Attaching a high pressure sterilizable tape to a first bottle cap having perforated holes in the edge region; Sealing the bottle with the bottle lid to which the tape is attached, and sterilizing the bottle; Inoculating the mushroom seeds into the medium in the sterilized bottle; Culturing the inoculated mushroom seeds; After completion of the seed culture, removing the first bottle cap and replacing the second bottle cap with a perforated hole in the edge portion; Placing the cultivation bottle containing the replaced second bottle cap in a cultivation room, and fingering the mushroom; And a step of growing the above-mentioned mushroom.

In the mushroom weeding method of the present invention, the cultured mushroom may have a uniform shape of the fruit body.

In the method for bottling mushrooms according to the present invention, the bottle lid is flat and circular in the upper part and the lower part, and a cylindrical hole may be formed in the edge part so that the foot of the mushroom can grow and grow.

In the mushroom bottle-making method of the present invention, the bottle lid can be modified in various ways depending on the shape of the fruiting body to be obtained. The bottle lid is square, and the foot of the mushroom can grow on the edge portion A square hole may be drilled.

In the method according to the present invention, the square may be triangular, rectangular, pentagonal, hexagonal, octagonal, twelvangular, and the like, but is not limited thereto.

In the method for treating a mushroom according to the present invention, the mushroom may include a mushroom, a mushroom, a mushroom, and a mushroom.

In the method for growing a mushroom according to the present invention, the step of growing the mushroom may be carried out at a temperature of 16 to 19 DEG C, a carbon dioxide concentration of 700 to 900 ppm, an illumination of 300 to 500 lux, and a relative humidity of 85 to 95% .

In the method of bottling a mushroom of the present invention, it is preferable that the bottle lid has one hole.

In the mushroom weaving method of the present invention, the medium may be prepared using oak sawdust or wheat bran, and the water content of the medium is preferably adjusted to 62-64% (w / w) The filling amount can be adjusted by adjusting to 650 ~ 750g when cultivating 1,100cc bottle. Particularly, it is desirable to control the chopping part of the entrance machine so that the depth of the chopping in the entrance is 7 to 8 mm from the upper part.

In the mushroom mashing method of the present invention, the mashing is performed rapidly and sterilization is performed immediately after completion. In this case, high-pressure sterilization is carried out at 121 DEG C for 100 minutes by a conventional method. After the sterilization treatment, it is preferable to cool again. The cooling temperature at this time is preferably cooled so that the medium temperature is 20 ° C or lower.

In the method according to the present invention, the inoculation amount of the mushroom strain is preferably 15 to 20 cc as the standard inoculum amount.

In the method for treating mushrooms according to the present invention, the culture conditions of the mushroom seedlings are maintained at a temperature of 20 to 23 ° C for about 27 to 30 days. When the whole surface of the mushroom culture becomes dark white, .

In the method according to the present invention, the relative humidity of the culture is preferably controlled to 65-70%, the concentration of carbon dioxide to 2,000 ppm or less, and the light condition is preferably controlled by dark culture as much as possible.

In the mushroom weaving method according to the present invention, it is preferable that the cultivation bottle is moved from the culture room to the workplace to perform the operation.

In the method of bottling the mushrooms of the present invention, the feet at the perforated portion of the lid from which the tape is removed, i.e., the mushroom generating step, may or may not be scraped. For example, in case of leaf mushroom, it is preferable not to scrape bacteria. In case of scraping bacteria, not only the foot is delayed but also the yield is reduced.

In the method of bottling the mushrooms of the present invention, mycelium in the culture bottle grows in the form of a hyphae bundle, which rises through the holes of the bottle lid, and grows in the flora.

In addition, the present invention provides a mushroom prepared by the above method.

The mushroom according to the present invention is characterized in that the shape of the fruit body is uniform.

Hereinafter, the present invention will be described in more detail by way of examples. EXAMPLES The following examples illustrate the invention and are not intended to limit the scope of the invention.

< Example  1> Preparation of culture medium, Admission , Sterilization and inoculation

First, a mixed material obtained by mixing oak sawdust, which is the main material of the culture medium, and wheat bran as nutrients, at a weight ratio of 85:15, was used as a culture medium.

The bottle lid was pierced with a hole having a diameter of 20 mm on the edge portion, and then a tape capable of sterilizing with high pressure was attached to the hole portion. The culture bottle was used with a culture container for 1,100 cc of polypropylene mushroom. Was adjusted to 62 ~ 64% (w / w), and the amount of the mixed medium was adjusted to be 650 ~ 700g.

Particularly, the depth of the chopper was adjusted to 7 ~ 8mm from the upper part when the patient was admitted.

The cultivation bottles completed the bottles were closed with bottles using a lid with a tape capable of sterilizing high pressure in the perforated holes. The bottle was sterilized using a high-pressure sterilizer and the sterilization conditions were treated at 121 占 폚 for 100 minutes.

After the sterilization treatment was completed, the sterilizer door was opened at 98 ° C, and steam of high temperature in the sterilizer was forcibly discharged to the outside for 15 minutes and then moved to the cooling chamber to cool the medium temperature to 20 ° C.

Thereafter, the seedling of the leaf mushroom was inoculated into the medium at 20 캜 in which the cooling was completed using an automatic inoculator. At this time, the inoculum amount was 20 cc.

Cultivated bottles were incubated at 22 ~ 23 ℃ for about 27 days. Culture humidity was 65 ~ 70% and ventilation was done once every 10 minutes for one hour.

< Example  2> Foot of mushroom, growth and harvest

After completing the culturing process according to Example 1, the tape adhered on the bottle lid was removed, and it was granulated in the cultivation room.

In the cultivation room, the slope was tilted at about 45 degrees and the edge of the bottle lid was directed upward. Growth conditions of the cultivation room were maintained at a temperature of 16 to 19 ° C, a concentration of carbon dioxide of 900 ppm or less, a light condition of 300 to 500 lux, and a relative humidity of 85 to 95%.

As described above, it was possible to harvest after 18 days by controlling the growth. The harvests were harvested when the mushrooms formed between the holes were fully deployed in the fist size on the bottle lid.

In this case, the cultivation days to harvest were 17-20 days, and the average yield per mushroom was 90 ~ 110g. The overall shape of the harvested mushrooms was uniform in the shape of a steamed bread, and there was almost no contamination by blue mold (a)).

< Example  3> Culture after completion  Harvesting fruiting bodies using a bottle lid perforated at the edge in the granulation phase

The lid of the culturing bottle completed in Example 1 was removed and replaced with a bottle lid having a perforated hole in the edge portion without being scraped, , And harvested fruit bodies with the holes facing upwards.

In this case, the number of days of cultivation was 17-20 days, and the average yield per mushroom was 90 ~ 110g. The overall shape of the harvested mushrooms was uniform in the shape of a spinach of circular shape, and there was almost no contamination due to blue fungi b)).

< Comparative Example  1> Culture after completion  Harvesting fruiting bodies using a bottle cap with perforated holes in the central region during the granulation phase

The lid of the culture bottle which had been cultivated according to the above Example 1 was removed and a perforated hole was formed in the central portion without performing bacterial scratching, except that a bottle lid having a perforated hole was used instead of the edge portion , And the seedlings were harvested by tilting the seedlings at an angle of about 45 degrees as in Example 2, with the positions of the holes facing upward.

The number of days of cultivation was 17 ~ 20 days, and the average yield per 100 rice paddies was 100 ~ 110g. However, the mushrooms did not occur due to pollution caused by blue fungi and the like (5-30%) (Fig. 4 (c) and (d)).

< Comparative Example  2> Harvesting fruiting body according to the conventional foot and growing process (when scraping is performed)

The lid of the culture bottle which had been cultivated in accordance with Example 1 was removed, except that the conventional bottle lid having no perforated hole was used. After the liquor was scraped off, it was granulated in a cultivation room, Mature fruiting body was harvested by controlling the temperature to 16 ~ 19 ℃, the concentration of carbon dioxide was 900ppm or less, the light condition was 300 ~ 500 lux, and the relative humidity was 85 ~ 95%.

The average number of cultivated mushrooms per day was 80 ~ 90g. The total morphology of harvested mushrooms was very diverse, and the degree of firmness and size uniformity of mushrooms was very poor.

< Comparative Example  3> The harvesting of fruiting body according to the conventional foot and the growing process (in the case of no scraping)

The mature fruiting bodies were harvested by carrying out the same procedure as in Comparative Example 2 except that the lid of the culture bottle after completion of cultivation was removed and no bacterial scratching was performed.

The total number of cultivated mushrooms ranged from 16 to 18 days, and the average yield per mushroom ranged from 90 to 100 g. The overall shape of the harvested mushrooms was very diverse, and the degree of symptom severity appear. In addition, the hardness and uniformity of the size of the mushrooms were poor.

< Experimental Example > Using a hole-drilled bottle cap Leaf  Mushroom Masturbation  Quantity characteristics and pollution rate survey

Table 1 shows the results of water content and contamination rate of the mushroom in the bottle using a bottle lid having perforations 20 mm in diameter at the central part and the edge part.

Hole
location Fruit body size (mm) Freshly colored Quantity
(g / bottle) Contamination rate
(%) Height Long length Short diameter L a b center 65.5 104.6 75.7 42.6 5.5 9.2 92.6 39.6 edge 51.3 100.5 79.7 34.6 4.8 7.5 99.0 0

* Test system: GWM20659, Completion days: 24 days, Length of stay: 9 days

As can be seen from the above results, the conventional bottling method (Comparative Examples 2 to 3) using the bottling method using the bottle lid having the perforated hole at the central portion (Comparative Example 1) or the bottle lid without the perforated hole (Examples 2 to 3) using the bottle cover according to the present invention is a useful method for preventing pollution caused by blue fungi and improving the productivity and merchantability of mushroom have.

The reason why the mushroom method according to the present invention can prevent contamination by blue fungi is that when a mushroom mat is formed from the edge portion and a hole is formed in the edge portion, the formation of the mushroom is quick, When using a bottle cap with a perforated hole in the center area, there is a perforation hole in the central part of the bottle, so that the hole is pierced, the yellow liquid water is caught in the area, and a pathogenic bacteria such as a blue fungus This is because pollution occurs.

According to the seedling growing method of the present invention, it is possible to provide a mushroom which is free from decay by germs, is excellent in quality, and has a constant size of fruiting body and can be standardized, so that it can be usefully used for growing farmers.

1: Mushroom cultivation bottle lid,
2: perforated hole for foot and growth
3: Mushroom grown on the bottle cap of mushroom cultivation

Claims (12)

Claims [1] A method for treating mushroom by using a bottle cap having perforated holes in an edge portion thereof,
Wherein the cultivation bottle having the bottle lid is inclined at an inclination of 45 DEG, and the cultivation is carried out with the opening facing upward. The method according to claim 1,
Preparing a mushroom culture medium and introducing the prepared culture medium into a cultivation bottle;
Attaching a high pressure sterilizable tape to a bottle cap having perforated holes in the edge region;
Sealing the bottle with the bottle lid to which the tape is attached, and sterilizing the bottle;
Inoculating the mushroom seeds into the medium in the sterilized bottle;
Culturing the inoculated mushroom seeds;
Removing the tape from the bottle cap after completing the seed culture;
Placing the cultivation bottle from which the tape has been removed in a cultivation room and fingering the mushroom; And
Wherein the step of growing the mushroom comprises culturing the mushroom. The method according to claim 1,
Preparing a mushroom culture medium and introducing the prepared culture medium into a cultivation bottle;
Attaching a high pressure sterilizable tape to a first bottle cap having perforated holes in the edge region;
Sealing the bottle with the bottle lid to which the tape is attached, and sterilizing the bottle;
Inoculating the mushroom seeds into the medium in the sterilized bottle;
Culturing the inoculated mushroom seeds;
After completion of the seed culture, removing the first bottle cap and replacing the second bottle cap with a perforated hole in the edge portion;
Placing the cultivation bottle containing the replaced second bottle cap in a cultivation room, and fingering the mushroom; And
Wherein the step of growing the mushroom comprises culturing the mushroom. [3] The method according to claim 1, wherein the cultivated mushroom has uniform fruiting bodies. The method according to claim 1, wherein the bottle lid has a flat top and a bottom and is circular, and a cylindrical hole is perforated so that the foot of the mushroom can grow and grow at the edge portion. . [3] The method according to claim 1, wherein the mushroom is selected from the group consisting of a mushroom, a mushroom, a mushroom, and a mushroom. [3] The method according to claim 2, wherein the step of growing the mushroom is carried out at a temperature of 16 to 19 DEG C, a carbon dioxide concentration of 700 to 900 ppm, an illumination of 300 to 500 lux, and a relative humidity of 85 to 95% Of mushroom using. 4. The method according to claim 3, wherein the step of growing the mushroom is carried out at a temperature of 16 to 19 DEG C, a carbon dioxide concentration of 700 to 900 ppm, an illumination of 300 to 500 lux, and a relative humidity of 85 to 95% Of mushroom using. [2] The method according to claim 1, wherein the bottle lid has one perforated hole. [2] The method according to claim 1, wherein the bottle lid is square, and a prismatic hole is perforated so that the foot of the mushroom can grow and grow. delete delete

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