KR101712478B1 - Composition for Improving Scalp Condition Using an Extract of Perilla frutescens, Etc. - Google Patents

Abstract

The present invention discloses a composition for improving scalp condition using a Perilla frutescens extract. The Perilla frutescens extract reduces the amount of scalp sebum in a human body application test, and exhibits stimulation-relieving activity by using sodium lauryl sulfate (SLS) or a hair dye agent in cell experiments and animal experiments.

Description

TECHNICAL FIELD The present invention relates to a composition for improving the scalp condition using a natural lysolecule extract,

The invention chair leaflets (perilla (Perilla The present invention relates to a composition for improving the condition of the scalp and the hair using the extract of the present invention.

The scalp is a specialized skin tissue covered with hair, composed of epidermis, dermis, and subcutaneous tissue, and the outermost tissue is the epidermis. The scalp is richer in hair follicles and blood vessels than other skin tissues, and the blood vessels and lymph vessels in the subcutaneous tissue supply nutrients and oxygen in the scalp to provide an environment in which hair can grow.

As the desire for beauty has increased in the modern society where living standard has been improved, the use of hair dye, permanent agent, shampoo, etc. is increasing for expressing various personality, and the degree of exposure to harmful substances from the outside is also increasing. Healthy scalp and hair have adequate sebum secretion, elasticity and gloss, but continuous use of hair dye and permanent agent and continuous exposure to external harmful substances disturb this homeostasis, causing scalp trouble or inflammation such as itching and stinging, Tapering, and hair loss.

In recent years, hair cosmetics have been specialized and upgraded by adding simple styling and cleaning functions to improve the hair and scalp condition to satisfy consumers' desire for healthy scalp and hair, Research is being actively conducted on the related products.

Korean Patent Laid-Open Publication No. 2015-0057332 discloses that Abies Koreana extract has dandruff suppression and itching sedative effect. Korean Patent No. 1345156 discloses that Amaranth and Lentil mixed extract are safe on the scalp, And Korean Patent Laid-Open Publication No. 2009-0056296 discloses that herbal medicine extracts such as ginseng, black bean, and seaweed have a hair root strengthening effect and the like.

The present invention discloses an effect of improving the scalp state of natural products such as natural leaves.

It is an object of the present invention to provide a composition for improving the scalp condition using a self-leaf extract or the like.

Other objects and specific objects of the present invention will be described below.

The present invention relates to (i) a self-leaf extract, (ii) rosemary ( Rosemarinus ) officinalis) extract, (iii) character leaflets and rosemary extract mixture, (iv) Here leaflets and rosemary extract, mixed fermented product, (v) character leaflets, rosemary, soybean (black soybean, Glycine max) and Houttuynia cordata (Houttuynia cordata ) was found to reduce the amount of scalp sebum in human body tests and to demonstrate the activity of stimulation with sodium lauryl sulphate (SLS) or a hair dye in cell and animal experiments. Here, SLS is a surfactant contained in shampoo, hair lotion, etc., and is known to cause skin irritation by destroying the cell membrane (Korean Dermatology Journal 35 (3): 491-498, 1997).

(Ii) rosemary extract, (iii) mixed lobular and rosemary extracts, (iv) mixed lobules and rosemary extract and fermented product And (v) a mixed extract / fermented product of a natural lobule, rosemary, black bean, and rhizome, as an active ingredient.

As used herein, the term "extract" refers to a stem, a leaf, a fruit, a flower and a root of a plant to be extracted with water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, methylene chloride, ethylene, acetone, , An extract obtained by leaching using chloroform, ethyl acetate, butyl acetate, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, Refers to an extract obtained by using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract. The extraction method is a method of extracting the supernatant from a supernatant, such as cold water, reflux, warming, Any method such as supercritical extraction can be applied. In the case of the fractionated extract, the crude extract is suspended in a specific solvent, and the fractions obtained by mixing and leaving with a solvent having a different polarity are adsorbed on a column packed with silica gel or the like, and a hydrophobic solvent, a hydrophilic solvent or a mixture thereof Quot; means a fraction obtained by using a solvent as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In the present specification, the term "active ingredient" alone means an ingredient which exhibits the desired activity or can exhibit activity together with a carrier which is not itself active.

In the present specification, the term "fermentation" refers to fermentation in a natural state without any artificial manipulation in fermenting microorganisms and fermentation conditions (e.g., presence of oxygen, temperature, humidity, etc.).

In the composition for improving scalp condition of the present invention, the active ingredient may be contained in any amount within a range that does not toxicity to the human body according to the purpose of use, formulation, etc. Specifically, it may be added in an amount of 0.01% 99.99% by weight.

The composition for improving scalp condition of the present invention can be identified as a cosmetic composition in a specific embodiment.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics, in the form of tablets or pills,

And any other ingredients used, such as cosmetics or adjuvants commonly used in the field of dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, surfactant- , A powder foundation, an emulsion foundation, a wax foundation, a spray, and the like. Specifically, the composition may be formulated into a form such as a hair tonic, a hair nutrition lotion, a scarf treatment, a hair treatment, a hair conditioner, a hair shampoo, Lt; / RTI >

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane, < / RTI > butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component. Specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be prepared according to the production method of a cosmetic composition known in the art, including its effective component and the above-mentioned carrier component.

The composition for improving scalp condition of the present invention can be grasped as a food composition in another specific embodiment.

The food composition of the present invention can be manufactured as health functional foods such as functional beverages, health supplements, special nutrition supplements, etc. The forms of foods include beverages such as tea, juice, carbonated beverage, ionic drink, milk, Food products such as processed oil, gum, rice cake, Korean lamb, bread, confectionery, cotton, etc., powders of health functional foods such as powders, tablets and capsules.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners. Such preservatives, emulsifiers and the like are well known in the art, and any of those known in the art can be used.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

It is preferable that the above preservatives, emulsifiers and the like are added and used in such a small amount as to attain the purpose to which they are added. As used herein, the term "trace amount " means, when expressed numerically, in the range of 0.0005 wt% to about 0.5 wt% based on the total weight of the food composition.

Food additives and food additives according to the Food Sanitation Law may be referred to in relation to additives and other additives that are required to be used depending on the type of food or type of food.

INDUSTRIAL APPLICABILITY As described above, the present invention can provide a composition for improving scalp condition using a self-leaf extract or the like. The composition of the present invention can be made into a cosmetic product, food or the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of sample

<Example 1> Preparation of a self-leaf extract

Five hundred folds by weight of 70% ethanol was added to 500 g of the lobulated leaf powder, which was then extracted at room temperature for 24 hours and then filtered with a 2 탆 filter paper. The resulting 70% ethanol filtrate was concentrated under reduced pressure and then lyophilized to obtain powdery black bean extract.

Example 2: Preparation of rosemary extract

Five times the weight of 70% ethanol was added to 500 g of the rosemary outpowder powder, which was then extracted at room temperature for 24 hours and then filtered through a 2 탆 filter paper. The resulting 70% ethanol filtrate was concentrated under reduced pressure and then lyophilized to obtain a powdery rosemary extract.

&Lt; Example 3 > Preparation of rosemary mixed extract

Five times the weight of 70% ethanol was added to a mixture of 250 g of the leaf lobate powder and 250 g of the rosemary outpowder powder, and the mixture was extracted at room temperature for 24 hours and then filtered through 2 탆 filter paper. The resulting 70% ethanol filtrate was concentrated under reduced pressure, and then lyophilized to obtain a powdery mixture of lobular and rosemary extracts.

&Lt; Example 4 > Preparation of rosemary mixed extract / fermented product

Five times the weight of 70% ethanol was added to a mixture of 250 g of the leaf lobate powder and 250 g of the rosemary outpowder powder, and the mixture was extracted at room temperature for 24 hours and then filtered through 2 탆 filter paper. The filtrate was spontaneously fermented at room temperature for 100 days, the fermentation broth was concentrated under reduced pressure, and then lyophilized to obtain a powdery foliar and rosemary mixed fermented product.

<Example 5> Here leaflets, rosemary, extract of black bean mixture and Houttuynia cordata, production fermentation water

Five times the weight of 70% ethanol was added to a mixture of 125 g of small leaf lobes, 125 g of rosemary seed powder, 125 g of black soybean powder, and 125 g of outpost powder, and the mixture was extracted at room temperature for 24 hours and then filtered with a 2 탆 filter paper. The filtrate was spontaneously fermented at room temperature for 100 days, the fermentation broth was concentrated under reduced pressure, and then lyophilized to obtain a powdery foliar and rosemary mixed fermented product.

< Experimental Example > Evaluating activity improvement of scalp condition and evaluation of activity of scalp stimulation relaxation

< Experimental Example  1> Evaluating activity improvement of scalp condition

<Experimental Example 1-1> Evaluation of activity of improving scalp sebum amount

Using the samples of the above examples, shampoo was prepared as shown in [Table 1] below and used for six adults each. The amount of sebum was measured before use, immediately after use and after 5 hours of use using Sebumeter. Germany . The results are shown in Table 2 below.

Shampoo composition and its content (% by weight) Ingredients / Manufacturing Example Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Comparative Example Lauryl polyglucoside 3 3 3 3 3 5 Ammonium laureth sulfate solution 4 4 4 4 4 2 Sodium lauryl sulfate 4 4 5 5 5 2 Isoamidopropylamine oxide 0.2 0.2 0.2 0.2 0.2 0.2 &Lt; RTI ID = 0.0 &gt; 0.3 0.3 0.3 0.3 0.3 0.3 Citric acid 0.2 0.2 0.2 0.2 0.2 0.2 Example 1 2.0 - - - - - Example 2 - 2.0 - - - - Example 3 - - 2.0 - - - Example 4 - - - 2.0 - - Example 5 - - - - 2.0 - Spices 0.3 0.3 0.3 0.3 3.0 0.3 Purified water Balance Balance Balance Balance Balance Balance * The above component content is expressed as% by weight.

Scalp sebum volume (/ / cm ² ) division Before use Immediately after use After 5 hours of use Comparative Example 187.27 28.92 132.64 Production Example 1 192.64 26.27 124.29 Production Example 2 189.62 27.94 127.54 Production Example 3 184.32 25.64 112.36 Production Example 4 188.94 24.42 108.23 Production Example 5 192.31 24.27 107.67

Referring to the results of the above Table 2, the products of the manufacturing examples were comparable to those of the products of the comparative examples and the amounts of the scalp immediately after use, but after 5 hours of use, the products of the examples of the present invention had a significantly lower effect of suppressing the scalp amount (Preparation Example 3), mixed extract / fermented product of Rosmarinus and Rosemary (Preparation Example 4), and mixed extract / fermented product (Production Example 5) of Rosa Lavender, Rosmarinus, And the degree of increase in scalp sebum was significantly lowered.

<Experimental Example 1-2> Sensory Evaluation on Scalp Itching and Hair Protection

Using the shampoo of the preparation example of the above Table 1, 18 applicants with scalp itching were subjected to the sensory evaluation of scalp itching relief and hair blurring. First, each preparation shampoo of Table 1 was used once a day for 2 weeks. After 2 weeks, the degree of scalp itching relief and the degree of hair softness were evaluated according to the 5-point scaling method. 3].

Sensory evaluation result division Scalp itching Hair softness Comparative Example 3.8 2.4 Production Example 1 3.2 3.2 Production Example 2 3.4 2.9 Production Example 3 2.8 3.6 Production Example 4 2.4 3.9 Production Example 5 2.2 4.1

< Experimental Example  2> Experiments on the activation of scalp stimulation

<Experimental Example 2-1> Protective activity against human fibroblast

(Dermatological Journal 35 (3): 491-498, 1997), which is known to be a substance that induces skin irritation, which is contained in shampoo using human fibroblast Hs68, inhibits cytotoxicity by sodium laurylsulfate (SLS) MTT assay.

Human fibroblast Hs68 was added at a concentration of 1 × 10 ⁵ cells / mL per 96 well plate, and the sample of 0.3% (w / v) and 0.002% (w / v) SLS were simultaneously treated and cultured for 3 days. Next, 100 μg of 3- (4,5-dimethylthiazol) -2,5-diphenyltetrazolium bromide was added and further cultured for 4 hours. Then, the medium was centrifuged at 1,000 rpm for 10 minutes and the medium was carefully removed. After adding 150 μl of DMSO (dimethylsulfoxide; Sigma, St. Louis, Mo., USA), the formazan precipitate produced by the reduction of MTT was dissolved in the microplate reader (Bio-TEK instrumenis, Inc., Winooski Vermont, WI, USA ) Was used to measure the absorbance at 540 nm.

The results are shown in Table 4 below as the mean value ± standard deviation of the cell survival rate relative to the untreated group in the triplicate experiments.

Human fibroblast survival rate (%)  Name of sample  Fibroblast survival rate (%) Sample-free treatment group 44.64 + - 7.92 Example 1 61.38 + - 7.48 Example 2 58.94 + - 8.82 Example 3 72.83 + - 4.96 Example 4 84.64 ± 6.27 Example 5 82.28 + - 7.42

The results of the above Table 4 show that all the samples of the Examples have an effect of canceling the toxic effect of SLS by human fibroblasts. In particular, here, the mixed extract of Lilium and Rosemary (Preparation Example 3), the mixture of Liliaceae and Rosemary Fermented product (Preparation Example 4), and the mixed fermented product (Preparation Example 5) of wild lobster, rosemary, black soybean and whitewash were remarkably high.

&Lt; Experimental Example 2-2 > Animal experiment for relieving hair dye stimulation

A female mouse C57BL / 6 at 6 weeks of age was purchased and used for the test after 10 days of quarantine and adaptation period. As the hair dye, a hair dye having the components and contents shown in the following Table 5 was used.

Hair dye composition and content division ingredient Content (% by weight)



1 Component Purified water Balance Sodium sulfide 0.5 Cetostearyl alcohol 6.0 Polyoxyethylene oleyl ether 5.0 Polyoxyethylene oleyl ether 5.0 Resorcinol 0.5 Ascorbic acid 0.5 Monoethanolamine 0.2 p-phenylenediamine 0.5 Ascorbic acid 0.5 ammonia 0.5
2 ingredients
Hydrogen peroxide (30%) 17.0 Phosphoric acid 0.1 Purified water 0.1

Specifically, the hair on the abdomen of the mouse was removed and 1% paraphenylenediamine (PPD) was applied for 3 days (three times in total), followed by a rest period of 1 week, The hair dye was applied to the ear of the mouse, and after 30 minutes, the hair dye was wiped off and the hair was rested for one week. Then, each sample of 3.0% (w / v) of the sample dissolved in physiological saline was applied three times at intervals of 1 hour, then the hair dye was applied again, and after 12 hours from the application of the hair dye, each sample of the above example was treated for 1 hour And applied three times at intervals. After 24 hours, the weight of the mouse ear was measured and the thickness of the ear was measured by H & E staining. In the comparative example, physiological saline not containing the sample of the example was used, and the results are shown in Table 6 below, with the mean value ± standard deviation of the triplicate experiments.

Ear weight (mg) and thickness (占 퐉) division Ear Weight (mg) Ear thickness (㎛) Comparative Example 15.76 ± 0.29 354.74 ± 18.25 Production Example 1 13.74 ± 0.63 265.64 + 24.27 Production Example 2 14.62 + - 0.42 272.84 ± 21.72 Production Example 3 12.82 + 0.47 259.73 + - 23.69 Production Example 4 10.64 ± 0.52 243.84 + - 23.37 Production Example 5 10.26 + - 0.46 246.92 + - 22.45

The results of the above Table 6 also show that the samples of the Examples have the effect of alleviating the hair dye stimulation. In particular, the effect of reducing the hair dye stimulation was high in the mixed fermented product of japanese lobule and rosemary (Preparation Example 4), and the mixed fermented product of japanese lobule, rosemary, black soybean and horseradish (Preparation Example 5).

Claims (2)

The mixture was prepared by adding 70% ethanol to the mixed mixture of lobular powder and rosemary pasta powder for 24 hours at room temperature. The mixture was filtered with 2 ㎛ filter paper, and the filtrate was spontaneously fermented at room temperature for 100 days. Wherein the cosmetic composition is a cosmetic composition for relieving scalp irritation.
The mixture was prepared by adding 70% ethanol to the mixed mixture of lobular powder and rosemary pasta powder for 24 hours at room temperature. The mixture was filtered with 2 ㎛ filter paper, and the filtrate was spontaneously fermented at room temperature for 100 days. Wherein the composition is a composition for relieving scalp irritation.