KR20150124900A - Cosmetic composition and health functional food for skin moisturizing comprising honeybush extract, its fraction or fermentative product as effective component - Google Patents
Cosmetic composition and health functional food for skin moisturizing comprising honeybush extract, its fraction or fermentative product as effective component Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
Description
The invention Honey bushing (Cyclopia intermedia , honeybush) fractions, fractions thereof or fermented products thereof as active ingredients, and a health functional food.
The skin is an organs covering the outside, consisting of three distinct layers of skin from the outside, the epidermis and the subcutaneous fat layer. The epidermis is mostly keratinized cells of middle layer squamous epithelium. The dermis, composed of matrix proteins such as collagen fibers and elastic fibers, is located beneath the epidermis, and the dermis contains blood vessels, nerves, and sweat glands. The subcutaneous fat layer is composed of adipocytes. The skin composed of the above-mentioned structure has a barrier function to protect the body from body temperature control and various external stimuli while blocking the inner moisture. To this end, it develops into a thin stratum corneum containing moisture through the process of differentiation in the epidermis of the skin.
Therefore, the degree of skin moisturization is recognized as an important factor in the field of cosmetics, skin science, and medicine. That is, the moisturizing condition of the stratum corneum is directly related to the appearance of the skin (flexibility and roughness of the skin) and is a measure of health condition. In addition, since the skin moisture content of the stratum corneum influences the skin membrane homeostasis related to the condition and permeability of the skin, the moisture absorption of the cosmetic or the medicine to be applied can be changed, and skin moisturization is an important factor (Esposito E et al , Int J Cosmet Sci, 29, p 39-47, 2007; Kasting GB et al., J Pharm Sci, 92, p 1624-1631, 2003).
In recent years, there have been a lot of cases of exposure to strong ultraviolet rays due to environmental pollution and the like, and as a result, the loss of skin moisturizing amount due to the increase in skin moisture has accelerated the aging of the skin and the incidence of the related diseases caused by skin damage is increasing. There is an increasing demand for a cosmetic composition or a health functional food which maintains the moisture retention of the skin so that the skin does not lose moisture and elasticity due to photoaging, and that the effect is maintained for a long time.
Meanwhile, Honeybush ( Cyclopia intermedia , honeybush) is an evergreen shrub belonging to Melianthaceae, a plant that grows only in a narrow region of the ridge of the eastern coast of the Cape region of South Africa. It is a very similar hub to rooibos. The name 'Honeybush' is the name of the flower, because it has a scent of honey. It is similar to rooibos, but the flavor is much more sweet. Honeybush is mainly used as a car, with few tannins, no caffeine, and various minerals (eg iron, potassium, calcium, copper, zinc, magnesium). It has long been used to treat colds, insomnia, and stomachaches, and is known to have an effect of lowering blood sugar. In this respect, the anti-diabetic effect of Honeybush extract in PCT / EP2008 / 052863 has been described and reported in Sissing, L. et al., Nutrition and cancer, v.63, no.4, pp.600-610 , 2011], it is described that the honeybush extract can control the differentiation of esophageal papilloma, but its skin moisturizing effect is not yet known.
SUMMARY OF THE INVENTION The present invention has been made in view of the above needs, and the present inventors have made intensive studies to develop a natural plant extract having a function of skin moisturizing function. As a result, it has been found that the extract of Honeybush, its fractions and fermented products It has been found that the skin moisturizing effect is superior to the skin moisturizing effect which suppresses or reduces the loss of skin moisture and protects the skin barrier, so that it can be effectively used as an effective ingredient of cosmetic composition for skin moisturizing and health functional food.
The invention Honey bushing (Cyclopia intermedia , honeybush) extract, a fraction thereof, or a fermented product thereof as an active ingredient.
In addition, the present invention provides a health functional food for skin moisturizing which contains as an active ingredient a honeybush extract, a fraction thereof or a fermented product thereof.
The invention Honey bushing (Cyclopia intermedia , honeybush) fractions, fractions thereof or fermented products thereof as active ingredients, and a health functional food. The composition and the health functional food according to the present invention have a skin moisturizing effect that suppresses or reduces the loss of skin moisture caused by ultraviolet irradiation.
FIG. 1 is a graph showing the effect of inhibiting transdermal water loss by administration of honey extract and fermented product (**** indicates that the transdermal water loss is statistically significant compared with the control, and **** indicates a p value of 0.0001 #### means that the amount of transdermal water loss of the honey extract and the fermented product is statistically significant, and #### means that the p value is less than 0.0001).
1. CON: control group;
2. UV: ultraviolet irradiation group;
3. HB EtOH EXT: group of 20% ethanol extract of honeybush;
4. HB H 2 O EXT: group of Honey bush water extract;
5. HB W: the group of fermented products of honeybees without animal milk; And
6. HB STC M: group of administration of fermented honey bush with animal milk.
FIG. 2 is a graph showing the results of evaluating the skin moisture content of the group administered with the honey extract and the fermented product (**** indicates that the skin moisture amount is statistically significant as compared with the control group, and **** indicates that the p value is less than 0.0001 ### and #### means that the skin moisture content of the honey extract and fermented product is statistically significant compared to the ultraviolet irradiation group, ### indicates that the p value is less than 0.001, #### is the p value Is less than 0.0001).
1. CON: control group;
2. UV: ultraviolet irradiation group;
3. HB EtOH EXT: group of 20% ethanol extract of honeybush;
4. HB H 2 O EXT: group of Honey bush water extract;
5. HB W: the group of fermented products of honeybees without animal milk; And
6. HB STC M: group of administration of fermented honey bush with animal milk.
FIG. 3 is a graph showing the results of evaluation of the mRNA expression level of involucrin by the Honeybush extract, the fraction and the fermented product (*, **, ***, and **** are the results of Honeybush * Indicates that the p value is less than 0.05, ** indicates that the p value is less than 0.01, *** indicates that the p value is less than 0.001, **** indicates that the p value is less than 0.0001. Further, # and ## indicate that the amount of phosphorylcholine mRNA expression of the honey broth fermented product is statistically significant compared to that of Honey bush water extract. , And ## indicates that the p value is less than 0.01).
1. CON: control group;
2. UV: ultraviolet irradiation group;
3. Water fraction of HB H 2 O fr: HB H 2 O EXT;
4. Butanol fraction of HB BuOH fr: HB H 2 O EXT;
5. Ethyl acetate fraction of HB EA fr: HB H 2 O EXT;
6. Methylene chloride fraction of HB MC fr: HB H 2 O EXT;
7. HB H 2 O EXT: group of Honey bush water extract; And
8. HB STC M: group of administration of honey bush fermented product using animal milk.
FIG. 4 is a graph showing the results of evaluation of the mRNA expression level of filaggrin by Honeybush extract, fractions and fermented products (*, ** and *** show the results of Honeybush extract, fraction and fermentation * Indicates that the p value is less than 0.05, ** indicates that the p value is less than 0.01, *** indicates that the p value is less than 0.001, and ## indicates Means that the amount of pilar green mRNA expressed in the honeycomb fraction compared to the honeycomb water extract is statistically significant, and ## indicates that the p value is less than 0.01).
1. CON: control group;
2. UV: ultraviolet irradiation group;
3. Water fraction of HB H 2 O fr: HB H 2 O EXT;
4. Butanol fraction of HB BuOH fr: HB H 2 O EXT;
5. Ethyl acetate fraction of HB EA fr: HB H 2 O EXT;
6. Methylene chloride fraction of HB MC fr: HB H 2 O EXT;
7. HB H 2 O EXT: group of Honey bush water extract; And
8. HB STC M: group of administration of honey bush fermented product using animal milk.
FIG. 5 is a graph showing the results of evaluation of the mRNA expression level of loricrin by the Honeybush extract, the fraction and the fermented product (*, **, ***, and **** are the concentrations of the Honey bush extract And * indicates that the p value is less than 0.05, ** indicates that the p value is less than 0.01, *** indicates that the p value is less than 0.001, and **** indicates that the amount of lorikreen mRNA expression of the fermented product is statistically significant. Means that the p value is less than 0.0001).
1. CON: control group;
2. UV: ultraviolet irradiation group;
3. Water fraction of HB H 2 O fr: HB H 2 O EXT;
4. Butanol fraction of HB BuOH fr: HB H 2 O EXT;
5. Ethyl acetate fraction of HB EA fr: HB H 2 O EXT;
6. Methylene chloride fraction of HB MC fr: HB H 2 O EXT;
7. HB H 2 O EXT: group of Honey bush water extract; And
8. HB STC M: group of administration of honey bush fermented product using animal milk.
The invention Honey bushing (Cyclopia intermedia , honeybush) extract, a fraction thereof, or a fermented product thereof as an active ingredient.
The honeycomb extract
a) extracting honeybees with an extraction solvent;
b) cooling the extract of step a), followed by filtration;
c) concentrating the filtered extract of step b) under reduced pressure; And
d) drying and pulverizing the concentrated extract of step c), but it is not limited thereto.
The honeybush of step a) can be used without limitation, such as grown or sold. The honeybush may utilize leaves, seeds, fruits, roots or aerial parts, but is not limited thereto.
It is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in the step c), but it is not limited thereto.
The drying in step d) is preferably, but not exclusively, reduced-pressure drying, vacuum drying, boiling drying, spray drying, far-infrared drying or freeze drying.
The extraction solvent is water, C < RTI ID = 0.0 > It is preferable to use at least one extraction solvent selected from the group consisting of C 4 lower alcohol, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. Most preferably, water is used but not limited thereto. As the lower alcohol, ethanol or methanol is preferably used, but it is not limited thereto.
The extraction solvent is preferably added by 2 to 10 times the total weight of the honeycomb, more preferably by 5 to 10 times, more preferably by 10 times, but not limited thereto.
The extraction method is preferably a shaking extraction or a reflux extraction, more preferably a reflux cooling extraction, but not always limited thereto.
The extraction temperature is preferably from room temperature to 100 ° C, but is not limited thereto. Further, the extraction time is preferably 1 to 3 hours, more preferably 1 hour, but is not limited thereto.
The number of times of extraction is preferably 1 to 3 times, more preferably 2 times, but is not limited thereto.
The honeycomb fermentation product
(a) inoculating the animal milk with lactic acid bacteria;
(b) mixing the carbon source and the honeycomb extract with the inoculum of step (a); And
(c) fermenting the mixture of step (b), but is not limited thereto.
Wherein the lactic acid bacteria of step a) are selected from the group consisting of Streptococcus thermophilus), but any lactic acid bacteria used for fermentation can be used.
The lactic acid bacterium of step a) is preferably cultivated in MRS (de Man, Rogosa, & Sharpe) medium. However, the medium is not limited thereto and any medium used for culturing lactic acid bacteria for fermentation may be used.
The lactic acid bacterium of step a) is preferably inoculated with 3 × 10 4 to 3 × 10 6 CFU / ml of lactic acid bacteria, more preferably, but not limited to, 3 × 10 5 CFU / ml of lactic acid bacteria.
The carbon source of step b) is preferably glucose or sugar, but is not limited thereto. The honeybush extract of step b) is preferably added in an amount of 3 to 10% (v / v) based on the total inoculum, more preferably 5 to 6% (v / v) v) is most preferably added, but not always limited thereto.
The fermentation of step c) is preferably carried out at a temperature of 35 to 38 ° C for 1 to 3 days, more preferably at a temperature of 37 to 38 ° C for 1 day, but is not limited thereto. Those skilled in the art can easily recognize that the fermentation temperature and time may be appropriately changed depending on the type of lactic acid bacteria.
The cosmetic composition of the present invention may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic liposome, , A skin, a lotion, a powder, an ointment, an essence, a spray or a cone stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.
In addition, the cosmetic composition may further contain, in addition to the honey extract, the fraction or the fermentation product of the present invention, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, ), Perfumes, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipids Or any other ingredient conventionally used in cosmetics, to name but a few.
In the cosmetic composition containing the honey extract, the fraction thereof or the fermentation product of the present invention, the extract of the present invention is added to the cosmetic composition usually contained in an amount of 1 to 15% by weight, preferably 2 to 10% by weight .
The cosmetic composition for moisturizing the skin of the present invention may further comprise a known skin moisturizing component in addition to the honeybush extract, its fractions or the fermented product. The skin moisturizing effect of the composition of the present invention can be further enhanced by including a raw material that provides additional skin health. In addition, when the raw material that contributes to skin health is added, skin safety due to combined use, ease of formulation , The stability of the active ingredients can be considered. The additional skin moisturizing ingredient is a functional ingredient disclosed in the Korean Food and Drug Administration, such as oil, sugar, polyol and the like, and the above-mentioned ingredient is not limited to a raw material for helping skin health.
In addition, the present invention relates to a method for producing Cyclopia intermedia , honeybush), a fraction thereof, or a fermented product thereof as an active ingredient.
The honeycomb fermentation product
(a) inoculating the animal milk with lactic acid bacteria;
(b) mixing the carbon source and the honeycomb extract with the inoculum of step (a); And
(c) fermenting the mixture of step (b), but is not limited thereto.
The health functional food of the present invention may be prepared by adding Honeybush Extract, its fraction or fermented product as it is, or with other food or food ingredients, and may be suitably prepared according to a conventional method. Examples of the food to which the Honeybush extract, its fraction or the fermented product can be added include dairy products including caramel, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramie noodles, other noodles, gums, , Various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense. That is, there is no particular limitation on the kind of the food.
The health functional foods include various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, , pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. The above components can be used independently or in combination.
In addition, the health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient, and the natural carbohydrates may be selected from the group consisting of glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, Polysaccharides such as dextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. The ratio of the natural carbohydrate is not critical, but is preferably 0.01 to 0.04 g, more preferably 0.02 to 0.03 g per 100 g of the composition of the present invention, but is not limited thereto.
Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.
The health functional food for skin moisturizing of the present invention may further comprise a known skin moisturizing ingredient in addition to the honeybush extract, its fraction or the fermented product. The inclusion of a raw material which contributes to additional skin health will further enhance the skin moisturizing effect of the health food of the present invention. In addition, when the raw material which helps the skin health is added, the skin safety according to the combined use, Ease of use, and stability of efficacy can be considered.
The additional skin moisturizing ingredient is a functional ingredient disclosed as a health functional food of the Korea Food & Drug Administration. Specifically, it is an oil, a sugar, a polyol, and the like. .
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.
Example One. Honeybush Preparation of extract
Honeybush was purchased from Renewal Life (http://www.renewallife.com) and samples were stored in a drug storage refrigerator maintained at a temperature of 5.5 ± 0.3 ° C and a humidity of 55 ± 5%. The honeybush extract was prepared by refluxing and cooling twice for 1 hour using water equivalent to 10 times of the total weight of Honeybush. The filtrate obtained by using the filter paper was subjected to removal of the solvent by using a vacuum concentrator, and then a honeybush extract was obtained. In the case of the 20% ethanol extract, an extract was obtained in the same manner as above.
Example 2. Honeybush Fermented Produce
In Example 2, a honeycomb fermented product was prepared. Honey bushing fermentation water producing process is MRS (de Man, Rogosa, Sharpe &) were cultured in a culture medium Streptococcus Thermo filler's (Streptococcus thermophilus), Lactobacillus 1 ㎖ (3 × 10 5 CFU / ㎖) of animal milk (milk white) were inoculated in 100㎖, sugar and honey 3g Bush extract 5% (v / v) was added, at a temperature of 37 ℃ And fermented for 1 day. In addition, fermentation without animal milk (white milk) was carried out in the same manner as above.
Experimental Example One. Honeybush Extract, objection Fraction or Fermented Evaluation of moisturizing efficacy
1) Experimental animal and sample administration
Seven-week-old male skeletal mice (male HR-1, hairless mice, Japan SLC, Inc.) were purchased from the central laboratory animals and used for 1 week. Animals in healthy state were used for the test by observing the general condition during the adaptation period. The breeding environment was maintained at a temperature of 23 ± 3 ° C, a humidity of 50 ± 5%, and a light period of 12 hours (07: 00-19: 00 / lighting time). The experimental animals were housed in a polycarbonate cage (200 × 320 × 145 mm, Three-shine Co., Daejeon, Korea) at a rate of 5 per group. And the drinking water which was sterilized by ultraviolet rays was fed free of negative water.
Sample administration was performed in the same manner as in the control (CON), ultraviolet treatment group (UV), the 20% ethanol extract group (HB EtOH EXT), the Honeybush water extract administration group (HB H 2 O EXT) (HBW), and a group of administration of fermented honeycomb flesh using animal milk (HB STC M). Samples were administered orally using a mouse mouse. The administration period was 8 weeks for a total of 5 days.
2) UV irradiation
UV irradiation was performed three times a week for 8 weeks except for the control group. UV irradiation was performed using a UV lamp (Mineralight UV Display lamp, UVP, USA). The ultraviolet irradiation dose was 60 mJ / cm 2 for 1 to 7 weeks and 90 mJ / cm 2 for 8 to 12 weeks for 12 weeks. The amount of ultraviolet radiation was measured by using a photometer (Delta OHM, Italy) and then adjusted to the irradiation time.
3) Percutaneous Water loss ( TEWL : transepidermal water loss ) Evaluation and analysis
The amount of transdermal water loss was calculated by area and time using the equipment of Tewameter Courage & Khazaka (Germany) at constant temperature and humidity (23 ℃, 50% relative humidity) (G / m 2 / hr) was read by a moisturizing electronic sensor, and the moisture content of the skin was measured. In order to confirm the moisturizing effect, transcutaneous water loss analysis was performed on the skin of reared mice in each experimental group.
The amount of transdermal water loss is the amount of water emanating from the skin. The higher the value, the lower the moisturizing function of the skin. This indicates that the inherent barrier function of the skin is impaired.
As a result, as shown in Fig. 1, it was confirmed that the skin barrier was damaged by ultraviolet irradiation and the skin moisturizing function was lost (p <0.0001), and the composition of the present invention was treated to prevent loss of skin water caused by ultraviolet rays (P <0.0001). The results were as follows.
4) Skin moisture measurement
Moisture content of the skin was measured using a Corneometer Courage & Khazaka, Germany instrument at constant temperature and humidity conditions (23 ° C, 50% relative humidity). The amount of moisture present in the epidermis of the skin was measured by measuring the degree of ion of water using a sensor, numerically calculating the amount of moisture, and measuring the moisture content.
The results obtained in this example were analyzed using statistical analysis program (one-way ANOVA and Student t-test).
As shown in FIG. 2, the honeycomb extract, its fractions or the fermented product group showed higher water content than the ultraviolet irradiation group. Based on these results, it was confirmed that the extracts of honeybees, their fractions or fermented products had skin moisturizing effect.
Experimental Example 2. Honeybush extract, Fraction And In the fermented water by Moisturizing factor mRNA Expression level evaluation
To investigate the effects of UV irradiation on the moisturizing factors of honeybees, the amounts of involucrin, filaggrin and loricrin mRNAs, which are moisturizing factors, were evaluated.
HaCaT cells were treated with each sample for 24 hours, irradiated with ultraviolet light (UV Crosslinker, Ultra Lum), and RNA was isolated according to the instruction using TRIzol reagent. The isolated RNA was dissolved in distilled water to which diethyl pyrocarbonate (DEPC) had been added, and the absorbance was measured at 260 nm and the concentration was quantified and used in the experiment. cDNA synthesis was performed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) according to the manufacturer's instructions. PCR was performed using TaqMan probes and primers (Involucrin, Filaggrin), and the expression levels were compared using β-actin as an internal control. The samples were divided into control, UV treatment group, Honey bush water extract group (HB H 2 O EXT), Honey bush fraction administration group (HB H 2 O fr, HB BuOH fr, HB EA fr, HB MC fr ) And Honeybush fermented water treatment group (HB STC M).
The results obtained in the present invention were analyzed using one-way ANOVA and Student t-test to determine the significance between the control group and the experimental group.
One) Honeybush extract, Fraction And In the fermented water by Inboluklein ( involucrin ) mRNA Expression level evaluation
Involucrin, which plays a role of skin moisturizing function, confirmed that the amount of involucrin mRNA expression decreased by UV irradiation. In the group treated with Honeybush extract, fraction and fermented product, It was confirmed that the expression of involucrin mRNA was increased compared to the ultraviolet irradiation group (Fig. 3).
That is, from the increase of the amount of involucrin mRNA expression, the honeycomb extract, fraction and fermentation product of the present invention significantly recover the moisturizing function damaged by ultraviolet irradiation, prevent skin dryness, It is confirmed that there is a moisturizing effect that prevents damage and maintains the moisture of skin epidermis.
2) Honeybush extract, Fraction And In the fermented water by Pillar Green ( filaggrin ) mRNA Expression level evaluation
Filaggrin also plays a role of skin moisturizing function like Involucrin. It was confirmed that the amount of filaggrin mRNA expression decreased by ultraviolet irradiation, and the honeybush extract, fraction and fermented product In the treated group, the expression of filaggrin mRNA was increased compared to the ultraviolet irradiation group (Fig. 4).
That is, the increase in the amount of filaggrin mRNA expression significantly restored the moisturizing function of the honeybush extract extract, fraction and fermentation product of the present invention, which was damaged by ultraviolet irradiation, and prevented skin dryness, To prevent moisture in the skin of the skin.
3) Honeybush extract, Fraction And In the fermented water by Loricrin ( loricrin ) mRNA Expression level evaluation
Loricrin also acts as a skin moisturizing function and it was confirmed that the amount of loricrin mRNA expression decreased by ultraviolet irradiation. In the group treated with Honeybush extract, fraction and fermented product, loricrin, mRNA expression was increased compared to the ultraviolet-irradiated group (Fig. 5).
That is, from the increase in the amount of loricrin mRNA expression, the honeycomb extract extract, fraction and fermentation product of the present invention significantly recover the moisturizing function damaged by ultraviolet irradiation, prevent skin dryness, To prevent moisture in the skin of the skin.
Manufacturing example One. Honeybush Extract, objection Fraction or The fermented product For moisturizing skin containing active ingredient Cosmetic Produce
1) Manufacture of flexible lotion
A flexible lotion containing the honeybush extract of the present invention, a fraction thereof or a fermented product thereof as an active ingredient was prepared.
10.00 parts by weight of honeybush extract, a fraction or fermented product thereof, 1.00 parts by weight of 1,3-butylene glycol, 0.05 parts by weight of disodium ditallow, 0.10 parts by weight of allantoin, 0.05 parts by weight of dipotassium glycyrrhizate, 0.01 part by weight of acid, 0.02 part by weight of sodium citrate, 1.00 parts by weight of glycerin-26, 2.00 parts by weight of arbutin, 1.00 parts by weight of hydrogenated castor oil, 30.00 parts by weight of ethanol, a preservative, And the softening longevity of the manufacturing cost was manufactured.
2) Manufacture of nutritional cream
A nutritional cream containing the honeybush extract of the present invention, a fraction thereof or a fermented product thereof as an active ingredient was prepared.
10.0 parts by weight of honeybush extract, its fraction or fermented product; 7.0 parts by weight of 1,3-butylene glycol; 1.0 part by weight of glycerin; 0.1 part by weight of D-Panthenol; 3.2 parts by weight of plant extract; 0.3 part by weight of magnesium aluminum silicate; 1.2 parts by weight of PEG-40 stearate; 2.0 parts by weight of stearic acid; 1.5 parts by weight of
3) Manufacture of Essence
According to the following composition ratio, purified water, honeybush extract, fraction or fermented product thereof, methylparaben, hyaluronic acid extract and glycerin were weighed and mixed at 80 ± 2 ° C to complete a water phase. In addition, propylparaben, lecithin and macadime mannut oil were weighed and mixed at 80 ± 2 ° C to complete an oil phase. Thereafter, the oil phase was added to the aqueous phase, and the two phases were mixed with each other to prepare an emulsion-type mixture. The mixture was added to the mixture and cooled to 35 DEG C to prepare a honeybush extract, its fraction or a fermented product .
7.58 parts by weight of purified water as an aqueous phase component; 8.00 parts by weight of honeybush extract, its fraction or fermented product; 0.15 part by weight of methylparaben; 2.50 parts by weight of hyaluronic acid extract; Glycerin and 8.00 parts by weight of glycerin.
0.10 parts by weight of propylparaben as an oily component; 0.60 part by weight of lecithin; 10.0 parts by weight of Matademia nut oil; And 0.07 parts by weight of fragrance as an additive.
Production Example 2. Preparation of health functional food for skin moisturizing containing honeybush extract, its fraction or fermented product as an active ingredient
1) Manufacture of flour food
0.5 to 5.0 parts by weight of the honeybush extract of the present invention, its fraction or the fermented product was added to wheat flour, and the mixture was used to prepare bread, cake, cookies, crackers and noodles.
2) Dairy products ( dairy products )
5 to 10 parts by weight of the honeybee extract of the present invention, its fractions or fermented product were added to milk, and various dairy products such as butter and ice cream were prepared using the milk.
3) Solar Produce
Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.
Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a grinder. The honeybee extract of the present invention, its fraction or the fermented product was concentrated under reduced pressure in a vacuum concentrator, dried by spraying and dried with a hot air drier, and pulverized with a granulator to a size of 60 mesh to obtain a dry powder.
The cereals, seeds, and honeybush extracts, fractions or fermented products thereof prepared above were blended in the following proportions. Cereals (30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley); Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame): Honeybush extract, its fraction or fermented product (3 parts by weight); Manure (0.5 parts by weight); (0.5 parts by weight)
4) Manufacture of chocolate
30.0 parts by weight of cacao butter; 30.0 parts by weight of whole milk powder; 41.85 parts by weight per minute; 0.45 parts by weight of lecithin; 0.1 part by weight fragrance; A fraction thereof, or a fermented product at a production ratio of 0.6 part by weight to prepare a honey containing honeybush extract, a fraction thereof, or a fermented product.
5) Health drink Produce
5 g of the extract of Honeybush Extract of the present invention, its fraction or the fermentation product of the present invention is homogenized in a homogenous state such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% And sterilized at the moment, and then packaged in small containers such as glass bottles and plastic bottles.
6) Vegetable juice Produce
Vegetable juice was prepared by adding 5 g of the honeybush extract of the present invention, its fraction or fermented product to 1,000 ml of tomato or carrot juice.
7) Manufacture of fruit juice
Fruit juice was prepared by adding 1 g of the honeybush extract, its fraction or fermented product of the present invention to 1,000 ml of apple or grape juice.
Claims (12)
(a) inoculating the animal milk with lactic acid bacteria;
(b) mixing the carbon source and the honeycomb extract with the inoculum of step (a); And
(c) fermenting the mixture of step (b).
(a) inoculating the animal milk with lactic acid bacteria;
(b) mixing the carbon source and the honeycomb extract with the inoculum of step (a); And
(c) fermenting the mixture of step (b).
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| KR20140051263 | 2014-04-29 | ||
| KR1020140051263 | 2014-04-29 |
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| KR101761125B1 KR101761125B1 (en) | 2017-07-25 |
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| KR1020150058721A KR101761125B1 (en) | 2014-04-29 | 2015-04-27 | Cosmetic composition and health functional food for skin moisturizing comprising honeybush extract, its fraction or fermentative product as effective component |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101988878B1 (en) * | 2017-12-19 | 2019-06-14 | 강원대학교산학협력단 | Composition for enhanced flavor, antioxidation and immune function comprising fermented garlic using solid state fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102091873B1 (en) * | 2019-10-22 | 2020-03-23 | 손찬락 | Functional food composition including artemisia princeps extract and natural fermented soybean extract and method for preparing thereof |
| KR102290291B1 (en) | 2020-04-02 | 2021-08-13 | 건국대학교 글로컬산학협력단 | Composition for improving moisturizing skin containing fermented blueberry and black rice extracts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101295368B1 (en) * | 2011-07-18 | 2013-08-09 | 한국 한의학 연구원 | Composition for skin wrinkle improvement comprising extracts of honeybush extract or its fermentation solution as an active ingredient |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101988878B1 (en) * | 2017-12-19 | 2019-06-14 | 강원대학교산학협력단 | Composition for enhanced flavor, antioxidation and immune function comprising fermented garlic using solid state fermentation |
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