KR100950195B1 - Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 - Google Patents
Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 Download PDFInfo
- Publication number
- KR100950195B1 KR100950195B1 KR1020090023821A KR20090023821A KR100950195B1 KR 100950195 B1 KR100950195 B1 KR 100950195B1 KR 1020090023821 A KR1020090023821 A KR 1020090023821A KR 20090023821 A KR20090023821 A KR 20090023821A KR 100950195 B1 KR100950195 B1 KR 100950195B1
- Authority
- KR
- South Korea
- Prior art keywords
- cord blood
- stem cells
- cells
- umbilical cord
- fibronectin
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Immunology (AREA)
- Pregnancy & Childbirth (AREA)
- Neurology (AREA)
- Physical Education & Sports Medicine (AREA)
- Neurosurgery (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims (22)
- 제대혈로부터 분리된 단핵세포를 피브로넥틴이 포함된 배양 용기내에서, 우태아혈청(FBS) 20%, bFGF 1~40ng/ml, 아스코르브산 0.1~5.0㎍/ml, EGF 1~40ng/ml, 하이드로코르티손 0.1~1㎍/ml, IGF-I 1~40ng/ml 또는 VEGF 1~5ng/ml 및 헤파린 20~25㎍/ml를 포함하는 EGM-2 또는 SNU-1 배지를 이용하여 배양한 후 배양물로부터 줄기세포를 회수하는 것을 특징으로 하는, ZNF281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법.
- 제 1항에 있어서,상기 단핵세포는 제대혈을 Hetasep과 혼합하여 적혈구를 제거한 후 피콜상(Ficoll-plaque)을 이용하여 단핵세포를 분리하는 단계를 거쳐서 수득하는 것을 특징으로 하는 분리방법.
- 제 2항에 있어서,제대혈과 Hetasep의 혼합 비율은 제대혈 5ml당 0.5~2ml로 하는 것을 특징으로 하는 분리방법.
- 제 1항에 있어서,상기 제대혈은 분만 직후 회수한 제대혈, 분만 직후 회수하여 실온에서 12~48시간 동안 보관한 제대혈 또는 3~5℃에서 6~72시간 동안 보관한 제대혈인 것을 특징으로 하는 분리방법.
- 제 1항에 있어서,상기 피브로넥틴이 포함된 배양용기는 피브로넥틴은 배양 용기에 코팅되거나, 입자 형태 또는 3차원 구조물 형태로 배지에 포함된 것을 특징으로 하는 분리방법.
- 제 5항에 있어서,피브로넥틴이 배양 용기에 코팅된 경우, 피브로넥틴은 0.1∼1mg/ml로 포함되는 것을 특징으로 하는 분리방법.
- 제 1항에 있어서,상기 피브로넥틴은 동물로부터 유래된 것을 특징으로 하는 분리방법.
- 제 7항에 있어서,상기 동물은 사람인 것을 특징으로 하는 분리방법.
- 제 1항에 있어서,상기 피브로넥틴은 인공적으로 합성되거나, 생합성된 것을 특징으로 하는 분리방법.
- 제 1항에 있어서,상기 피브로넥틴은 피브로넥틴의 단편(fragment) 또는 펩타이드를 포함하는 것을 특징으로 하는 분리방법.
- 삭제
- 제 1항에 있어서,배양물로부터 줄기세포를 회수함에 있어서, 줄기세포의 면역학적 특성을 이용하여 분리하는 것을 특징으로 하는 분리방법.
- 제 1항에 의해 분리된, 하기 특징을 갖는 제대혈 유래 만능 줄기세포:(a) 전사조절인자인 ZNF281에 대하여 양성의 면역학적 특성을 나타냄;(b) 세포외기질이 코팅된 바닥에 부착되어 부착 후 5 내지 30일 사이에 선 형태 또는 구 형태의 세포 집락을 이루면서 증식함; 및(c) 30 ~ 45의 CPDL(cumulative population doubling level)을 보임.
- 제 13항에 따른 제대혈 유래 만능 줄기세포 또는 이로부터 분화된 세포를 함유하는 세포치료제.
- 삭제
- 삭제
- 삭제
- 삭제
- 삭제
- 삭제
- 삭제
- 제 13항에 있어서,하기 특징 중 적어도 한 특성을 추가로 갖는 것을 특징으로 하는 제대혈 유래 만능 줄기세포:(a) 전사조절인자인 c-myc에 대하여 양성의 면역학적 특성을 나타냄;(b) CD14, CD31, CD34, CD45 및 HLA-DR에 대하여 음성의 면역학적 특성을 나타냄;(c) 중배엽, 내배엽 및 외배엽의 세포로 분화 가능함; 및(d) TIMP-2, TGF-β, RANTES, CINC-3, EOTAXIN, GM-CSF, IFN-γ, IL-1b, IL-3, IL-6, IL-8, IL-10, IL12p40, IL13, IL-16, IP-10, Leptin, MCP-2, MIG, MIP-3a, b-NGFm, sTNFRI, PFGF-bb로 이루어진 그룹으로부터 선택된 적어도 하나의 사이토카인 또는 케모카인을 분비함.
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090023821A KR100950195B1 (ko) | 2009-03-20 | 2009-03-20 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
PCT/KR2009/002333 WO2010107159A1 (ko) | 2009-03-20 | 2009-05-01 | Znf281을 발현하는 제대혈 유래 줄기세포의 분리방법 및 대량배양 방법 |
PL10753642T PL2410046T3 (pl) | 2009-03-20 | 2010-03-03 | Sposób izolowania pluripotencjalnych komórek macierzystych pochodzących z krwi pępowinowej wykazujących ekspresję ZNF281 |
AU2010225612A AU2010225612B2 (en) | 2009-03-20 | 2010-03-03 | Isolating method for umbilical cord blood-derived pluripotent stem cells expressing ZNF281 |
PCT/KR2010/001338 WO2010107192A2 (ko) | 2009-03-20 | 2010-03-03 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
MYPI2011004459A MY160164A (en) | 2009-03-20 | 2010-03-03 | Isolating method for umbilical cord blood-derived pluripotent stem cells expressing znf281 |
CN201080016872.7A CN102395673B (zh) | 2009-03-20 | 2010-03-03 | 来自脐带血的表达znf281的万能干细胞的分离方法 |
US13/257,961 US10584312B2 (en) | 2009-03-20 | 2010-03-03 | Isolating method for umbilical cord blood-derived pluripotent stem cells expressing ZNF281 |
JP2012500708A JP5995720B2 (ja) | 2009-03-20 | 2010-03-03 | Znf281を発現する臍帯血由来の万能幹細胞の分離方法 |
EP10753642.7A EP2410046B1 (en) | 2009-03-20 | 2010-03-03 | Isolating method for umbilical cord blood-derived pluripotent stem cells expressing znf281 |
ES10753642T ES2722930T3 (es) | 2009-03-20 | 2010-03-03 | Procedimiento de aislamiento para células madre pluripotentes derivadas de sangre umbilical que expresan ZNF281 |
RU2011142170/10A RU2511417C2 (ru) | 2009-03-20 | 2010-03-03 | Способ выделения происходящих из пуповинной крови плюрипотентных стволовых клеток, экспрессирующих znf281 |
JP2015000016A JP6038962B2 (ja) | 2009-03-20 | 2015-01-05 | Znf281を発現する臍帯血由来の万能幹細胞の分離方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090023821A KR100950195B1 (ko) | 2009-03-20 | 2009-03-20 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020090086315A Division KR101158664B1 (ko) | 2009-09-14 | 2009-09-14 | Znf281을 발현하는 제대혈 유래 줄기세포의 대량 배양방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR100950195B1 true KR100950195B1 (ko) | 2010-03-29 |
Family
ID=42183944
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020090023821A KR100950195B1 (ko) | 2009-03-20 | 2009-03-20 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
Country Status (11)
Country | Link |
---|---|
US (1) | US10584312B2 (ko) |
EP (1) | EP2410046B1 (ko) |
JP (2) | JP5995720B2 (ko) |
KR (1) | KR100950195B1 (ko) |
CN (1) | CN102395673B (ko) |
AU (1) | AU2010225612B2 (ko) |
ES (1) | ES2722930T3 (ko) |
MY (1) | MY160164A (ko) |
PL (1) | PL2410046T3 (ko) |
RU (1) | RU2511417C2 (ko) |
WO (2) | WO2010107159A1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012033352A3 (ko) * | 2010-09-08 | 2012-06-28 | 주식회사 강스템홀딩스 | 말과동물 양수 유래 다분화능 줄기세포 및 그 제조방법 |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100950195B1 (ko) | 2009-03-20 | 2010-03-29 | 서울대학교산학협력단 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
US8940294B2 (en) | 2012-03-02 | 2015-01-27 | Tissuetech, Inc. | Methods of isolating and culturing stem cells |
CN103396995B (zh) * | 2013-07-16 | 2015-09-23 | 广州赛哲生物科技有限公司 | 一种筛选乳腺癌干细胞的三维培养方法 |
WO2015064795A1 (ko) * | 2013-11-01 | 2015-05-07 | 주식회사 비비에이치씨 | 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포 |
SG10201912086QA (en) * | 2015-07-14 | 2020-02-27 | Medimmune Llc | Compositions and methods for treating cancer |
US10384207B2 (en) | 2015-07-21 | 2019-08-20 | Neuro Probe Incorporated | Assay apparatus and methods |
WO2017123312A2 (en) | 2015-09-02 | 2017-07-20 | Regeneration Worldwide Company, Inc. | Composition and methods of using umbilical cord lining stem cells |
CN105087494A (zh) * | 2015-09-11 | 2015-11-25 | 中国人民解放军第四军医大学 | 一种乳腺癌干细胞的培养方法 |
EP3385368B1 (en) * | 2015-12-03 | 2023-03-01 | Takara Bio Inc. | Method for producing mesenchymal stem cells |
JP6912789B2 (ja) * | 2016-03-16 | 2021-08-04 | 株式会社日本触媒 | 神経幹細胞の培養方法、およびニューロスフェロイドの形成方法 |
EP3490572A4 (en) * | 2016-08-01 | 2020-05-13 | Embryogenesis Pty Ltd | SKIN CARE FORMULA |
KR101798217B1 (ko) * | 2016-10-21 | 2017-11-15 | 차의과학대학교 산학협력단 | 만능줄기세포로부터 유래된 중간엽줄기세포를 선별하는 방법 |
RU2645255C1 (ru) * | 2016-12-16 | 2018-02-19 | Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр акушерства, гинекологии и перинатологии имени академика В.И.Кулакова" Министерства здравоохранения Российской Федерации | Способ получения биобезопасной культуры мезенхимальных стволовых клеток из ворсин хориона человека |
CN109666630A (zh) * | 2017-10-17 | 2019-04-23 | 澳门大学 | 多能干细胞分化为间充质干细胞的方法及其培养基和应用 |
CN109112099A (zh) * | 2018-08-30 | 2019-01-01 | 丰泽康生物医药(深圳)有限公司 | 一种提高单核细胞向多潜能细胞转化的无血清培养基 |
CN111424014B (zh) * | 2019-11-22 | 2022-03-29 | 上海交通大学医学院附属第九人民医院 | 人颈静脉球副神经节瘤永生化细胞株培养基 |
TW202208613A (zh) * | 2020-05-05 | 2022-03-01 | 美商加速生物科學有限公司 | 非永生的多潛能幹細胞 |
CN112430626A (zh) * | 2020-11-27 | 2021-03-02 | 成都康景生物科技有限公司 | 一种基因修饰的脐间充质干细胞、制备方法及应用 |
US11674090B1 (en) | 2021-11-30 | 2023-06-13 | Honeywell International Inc. | Energy optimization in fluid catalytic cracking and dehydrogenation units |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL143085A0 (en) * | 1998-12-04 | 2002-04-21 | Naval Medical Res Ct | Human brain endothelial cells and growth medium and method for expansion of primitive cd34+cd38 - bone marrow stem cells |
US6610535B1 (en) | 2000-02-10 | 2003-08-26 | Es Cell International Pte Ltd. | Progenitor cells and methods and uses related thereto |
US20040005559A1 (en) * | 2000-07-24 | 2004-01-08 | Loring Jeanne F. | Markers of neuronal differentiation and morphogenesis |
EP1491093B1 (en) * | 2001-02-14 | 2013-07-31 | ABT Holding Company | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
US7736892B2 (en) * | 2002-02-25 | 2010-06-15 | Kansas State University Research Foundation | Cultures, products and methods using umbilical cord matrix cells |
TWI288779B (en) * | 2002-03-28 | 2007-10-21 | Blasticon Biotech Forschung | Dedifferentiated, programmable stem cells of monocytic origin, and their production and use |
WO2004033396A2 (en) * | 2002-10-08 | 2004-04-22 | American National Red Cross | Method for enriching adherent monocyte populations |
US20030224411A1 (en) * | 2003-03-13 | 2003-12-04 | Stanton Lawrence W. | Genes that are up- or down-regulated during differentiation of human embryonic stem cells |
US20060223177A1 (en) * | 2003-06-27 | 2006-10-05 | Ethicon Inc. | Postpartum cells derived from umbilical cord tissue, and methods of making and using the same |
JPWO2005056778A1 (ja) | 2003-12-11 | 2007-07-12 | 独立行政法人理化学研究所 | 造血幹細胞の分化抑制又は増殖方法 |
WO2006012404A2 (en) * | 2004-07-20 | 2006-02-02 | Case Western Reserve University | Novel cell populations and uses thereof |
KR100697326B1 (ko) * | 2005-12-02 | 2007-03-20 | 재단법인서울대학교산학협력재단 | Oct4 발현능을 가지는 제대혈 유래 다분화능 성체줄기세포 및 그의 제조방법 |
WO2008110570A1 (en) | 2007-03-13 | 2008-09-18 | Medizinische Universität Graz | Method to study proliferation of endothelial progenitor cells and the potential influence of compounds on their proliferation behaviour |
RU2343928C1 (ru) * | 2007-04-03 | 2009-01-20 | Открытое акционерное общество "Институт стволовых клеток человека" | Способ получения ядросодержащих клеток из пуповинной крови |
WO2008149129A1 (en) * | 2007-06-08 | 2008-12-11 | Nova Thera Limited | Cell expansion |
KR100950195B1 (ko) | 2009-03-20 | 2010-03-29 | 서울대학교산학협력단 | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 |
-
2009
- 2009-03-20 KR KR1020090023821A patent/KR100950195B1/ko active IP Right Grant
- 2009-05-01 WO PCT/KR2009/002333 patent/WO2010107159A1/ko active Application Filing
-
2010
- 2010-03-03 JP JP2012500708A patent/JP5995720B2/ja active Active
- 2010-03-03 AU AU2010225612A patent/AU2010225612B2/en active Active
- 2010-03-03 RU RU2011142170/10A patent/RU2511417C2/ru active
- 2010-03-03 US US13/257,961 patent/US10584312B2/en active Active
- 2010-03-03 CN CN201080016872.7A patent/CN102395673B/zh active Active
- 2010-03-03 EP EP10753642.7A patent/EP2410046B1/en active Active
- 2010-03-03 MY MYPI2011004459A patent/MY160164A/en unknown
- 2010-03-03 WO PCT/KR2010/001338 patent/WO2010107192A2/ko active Application Filing
- 2010-03-03 PL PL10753642T patent/PL2410046T3/pl unknown
- 2010-03-03 ES ES10753642T patent/ES2722930T3/es active Active
-
2015
- 2015-01-05 JP JP2015000016A patent/JP6038962B2/ja active Active
Non-Patent Citations (4)
Title |
---|
Cell, Vol. 132, pp.1049-1061.* |
Contemporary Cardiol. Stem Cells Myocardial Regeneration, pp.67-81.* |
Cytotechnology, Vol. 54, pp.121-133.* |
Exp. Hematol., Vol.34, pp.1589-1595.* |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012033352A3 (ko) * | 2010-09-08 | 2012-06-28 | 주식회사 강스템홀딩스 | 말과동물 양수 유래 다분화능 줄기세포 및 그 제조방법 |
KR101380561B1 (ko) | 2010-09-08 | 2014-04-02 | 주식회사 강스템바이오텍 | 말과동물 양수 유래 다분화능 줄기세포 및 그 제조방법 |
US9290739B2 (en) | 2010-09-08 | 2016-03-22 | Kang Stem Biotech Co., Ltd. | Equine amniotic fluid-derived multipotent stem cells and a method for producing the same |
Also Published As
Publication number | Publication date |
---|---|
CN102395673A (zh) | 2012-03-28 |
ES2722930T3 (es) | 2019-08-20 |
WO2010107192A3 (ko) | 2011-02-24 |
JP2012520671A (ja) | 2012-09-10 |
RU2511417C2 (ru) | 2014-04-10 |
EP2410046B1 (en) | 2019-01-23 |
AU2010225612B2 (en) | 2014-06-26 |
JP6038962B2 (ja) | 2016-12-07 |
EP2410046A4 (en) | 2013-02-27 |
PL2410046T3 (pl) | 2019-10-31 |
RU2011142170A (ru) | 2013-04-27 |
EP2410046A2 (en) | 2012-01-25 |
WO2010107159A1 (ko) | 2010-09-23 |
AU2010225612A1 (en) | 2011-11-10 |
JP2015091254A (ja) | 2015-05-14 |
US10584312B2 (en) | 2020-03-10 |
US20120021509A1 (en) | 2012-01-26 |
JP5995720B2 (ja) | 2016-09-21 |
MY160164A (en) | 2017-02-28 |
WO2010107192A2 (ko) | 2010-09-23 |
CN102395673B (zh) | 2015-07-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR100950195B1 (ko) | Znf281을 발현하는 제대혈 유래 만능 줄기세포의 분리방법 | |
EP2374871B1 (en) | Pluripotent stem cells, method for preparation thereof and uses thereof | |
US7534606B2 (en) | Placental stem cell and methods thereof | |
US7101710B2 (en) | Two-stage culture protocol for isolating mesenchymal stem cells from amniotic fluid | |
US20100105132A1 (en) | Human Mesenchymal stem cells and preparation thereof | |
US20030152558A1 (en) | Methods and compositions for the use of stromal cells to support embryonic and adult stem cells | |
CN110468099B (zh) | 一种骨髓间充质干细胞培养基及其应用 | |
KR20210044749A (ko) | 무혈청 배지 조성물 | |
KR101896803B1 (ko) | 인간 만능줄기세포로부터 중간엽 줄기세포로의 분화 유도 생산율을 증가시키는 방법 및 이에 의해 생성된 중간엽 줄기세포 | |
KR101627907B1 (ko) | Znf281을 발현하는 제대혈 유래 줄기세포의 대량 배양방법 | |
KR102142254B1 (ko) | 3,4''-다이하이드록시플라본을 이용한 소변줄기세포의 분리 효율 향상 및 소변줄기세포유래 만능줄기세포의 조혈줄기세포 분화 효율을 촉진시키는 방법 | |
JP7198524B2 (ja) | スフェロイドの製造方法および多能性幹細胞マーカーを発現させる方法 | |
KR101158664B1 (ko) | Znf281을 발현하는 제대혈 유래 줄기세포의 대량 배양방법 | |
US20200131480A1 (en) | Method For Enhancing Proliferation Capability Of Stem Cells Using Ethionamide | |
KR20120030515A (ko) | Znf281을 발현하는 제대혈 유래 줄기세포의 대량 배양방법 | |
KR100818216B1 (ko) | Cripto-1 및 Oct4를 동시 발현하는 인간 지방조직 유래 다분화능 줄기세포 또는 종양 줄기세포 및 그 확인방법 | |
KR100818213B1 (ko) | 파골세포 기반 적소 유사 구조에 의해 증식능이 증가된인간 제대혈 유래 다분화능 줄기 세포 및 그 제조방법 | |
TWI307717B (en) | Placental stem cell and methods thereof | |
KR20190040957A (ko) | 분화된 심실 근육세포의 성숙방법 | |
KR20180123794A (ko) | 분화된 심실 근육세포의 성숙방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
A302 | Request for accelerated examination | ||
E902 | Notification of reason for refusal | ||
A107 | Divisional application of patent | ||
E90F | Notification of reason for final refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20130326 Year of fee payment: 4 |
|
FPAY | Annual fee payment |
Payment date: 20140319 Year of fee payment: 5 |
|
FPAY | Annual fee payment |
Payment date: 20141212 Year of fee payment: 6 |
|
FPAY | Annual fee payment |
Payment date: 20170103 Year of fee payment: 8 |
|
FPAY | Annual fee payment |
Payment date: 20180112 Year of fee payment: 9 |
|
FPAY | Annual fee payment |
Payment date: 20190109 Year of fee payment: 10 |
|
FPAY | Annual fee payment |
Payment date: 20200306 Year of fee payment: 11 |