KR100813187B1 - Composition for preventing and treating obesity comprising natural herb extracts - Google Patents

Abstract

A composition comprising an extract of natural herbs is provided to induce the body weight increase inhibition or body weight decrease, thereby being used for preventing and treating obesity. A composition for preventing and treating obesity comprises an extract of a mixture consisting of 1.0-30.0 parts by weight of Pinus densifloraSIEBOLD et ZUCCARINI, 1.0-20.0 parts by weight of Morus alba L., 1.0-20.0 parts by weight of Betula Platyphylla Sukatvar.japonica Hara, 1.0-30.0 parts by weight of Chaenomeles Sinensis Koeln and 1.0-20.0 parts by weight of Lonicera Japonica Thumb. A method for preparing the composition comprises the steps of: (a) cutting Pinus densifloraSIEBOLD et ZUCCARINI, Morus albaL., Betula Platyphylla Sukatvar.japonica Hara, Chaenomeles Sinensis Koeln and Lonicera Japonica Thumb finely; (b) extracting the cut mixture with water at a temperature of 50-70 deg.C and then subjecting the extract to ultrasonic wave treatment; (c) heating the extract at a temperature of 90-110 deg.C to evaporate; and (d) cooling the evaporated vapor using a cooling device. Further, a step of drying the cooled vapor at -35--45‹C, and manufacturing herbal dried powder is additionally comprised.

Description

Composition for preventing and treating obesity comprising natural herb extracts}

1 is a manufacturing process diagram of a suspension composition of the present invention.

2 is a graph showing the mouse weight inhibition effect of the suspension of the present invention.

Figure 3 is a graph showing the effect of reducing the body fat mass and body fat percentage of the suspension of the present invention.

4 is a graph showing the effect of the suspension of the present invention on the hip circumference and subcutaneous fat thickness in humans.

The present invention relates to a herb extract composition having the ability to prevent and inhibit obesity, more specifically pine needle (Pinus densiflora SIEBOLD et ZUCCARINI), upper leaf (and / or upper extremity) (Morus albaL.), Cotyledon (Betula Platyphylla Sukatvar.japonica Hara), The present invention relates to a composition for the prevention and treatment of obesity consisting of extracts of Juniperus Chinensis, Chaenomeles Sinensis Koeln and Lonicera Japonica Thumb.

Current treatments for obesity include calorie intake, increased exercise, lifestyle, medication, or gastrectomy. Among them, calorie control methods are mostly dietary fiber, and diarrhea foods and herbal extracts have not been scientifically proven.

Three types of drugs that are approved by the FDA for weight loss are Oristat, Xenical, Phentermine, Fastin, Zantryl, and Sibutramine, Reductil, Meridia. have. Orlistat (Xenical) reduces weight by preventing the absorption of about 30% of the fat in food, and phentermine and sibutramine are drugs that suppress appetite. Orlistat (Xenical) binds to lipase in the digestive tract, reducing the breakdown of fat in the small intestine, preventing the absorption of fat, so that undigested fat remains in the intestine, causing a number of side effects. It may be accompanied with various side effects such as abdominal pain, fatty stool, nausea, depression, leg pain, etc., and the more fats in the foods to be eaten, the greater the side effects.

Looking at the patents recently published and registered for the purpose of preventing and treating abandonment and treatment of hyperlipidemia by using herbal medicines, sesame fiber, or seaweed extract, Korean Patent No. 10-0499384 discloses Hwang Jung, Baek Ha Shou, Du Chung, Taxa, Disclosed is a method for preparing an herbal extract powder having excellent anti-hyperlipidemic activity using shiho, wolfberry, hyssop, nuro, moon dog, samchil-geun, myrrh, seaweed, mountain breeding, yeojeongsil, black jima, and zhaogapi.

Republic of Korea Patent 10-0624488 ginseng (Panax ginseng C. A. Meyer), dermis (Citrus unshiuMarcovich), upper lobe (and / or upper limb) (Morus albaL.), Euiin (Coix lachoryma-jobiLinne var. Ma-yuen STAPF), Poria cocosWOLF, Polyporus umbellatus FRIES, Acanthopanax sessiliflorum SEEMAN, Eucommia ulmoidesOLIVER, Rehmannia glutinosa LIBOSCHITZ var. Disclosed is a composition for the prevention and treatment of obesity consisting of extracts of Benincasa hispidaCOGNIAUX and pine needles (Pinus densiflora SIEBOLD et ZUCCARINI).

Republic of Korea Patent 10-0504124 is the main ingredient of Gilkyung (Pearly Bellflower), Uiin, Sukjihwang, Cheongung, Cha-eup, and leaf mushrooms, and contains various herbal medicines, food ingredients, vitamins and minerals, and other food additives as supplementary ingredients. There is disclosed a composition that can suppress and prevent various adult diseases.

Republic of Korea Patent 10-0645385 discloses a composition for inhibiting obesity as an active ingredient melissa leaf extract, jinjin wormwood extract, mulberry leaf extract.

Korean Patent Publication No. 10-2006-0094228 discloses a powder, hot water extract or ethanol extract of a plant containing a large amount of bioflavonoid-based material, polyphenol-based material or dietary fiber, and then alone or mixed with them to produce meat, meat products or dairy products. Disclosed is a method for manufacturing a food for preventing obesity and hyperlipidemia using a product.

Republic of Korea Patent 10-0597033 is persimmon, strawberry, banana, chaff, goji berry, cornus, safflower seed, bokbunja, pine needles, sprouts, stone parsley, barley sprout, cabbage, kale, buttercup, green tea leaf, ganoderma lucidum, mortar calcium, seaweed calcium, cucumber , Deodeok, chitosan, roasted salt, vitamin E, vitamin A, chlorella, etc. Constitution has been disclosed by the constituents for the treatment by the composition, and many other compositions can be seen to use health supplements.

Looking at the composition in detail, it can be found in other herbal compositions that have been used for the prevention and treatment of obesity, ginseng, licorice, goji berry, tofu, sukjijihwang, cheongung, leek, buttercup, bokbunja, ginger, lotus root, gold silver flower, Ogapi, There are wormwood, bellflower, donkey, and celestial arch. Comparing the composition except these substances, it can be seen that there are pineal leaves, upper leaves, and the like in the specifically seen composition for the purpose of prevention and treatment of the uterus.

Accordingly, the present invention adds pineal leaves and upper leaves, which are effective in obesity, to the composition that is included in Chinese medicine, and also known as birch, juniper, tree, and silver coins, which are recognized as substances capable of enhancing the prevention and treatment effect of obesity. The added composition was found to be more effective in inhibiting weight loss or increase, and completed the present invention.

Therefore, the main object of the present invention is to provide a composition that can be used for the prevention and treatment of obesity by efficiently inducing weight gain inhibition or reduction containing herbal extracts.

Another object of the present invention is to provide an effective method for preparing a composition that can be used for the prevention and treatment of obesity.

According to one aspect of the present invention, the present invention provides a composition for the prevention and treatment of obesity, containing the extract of pine needles, upper leaves, birch, neck, and Lonicera Japonica Thumb as an active ingredient. The present invention has been found for the first time that extracts combining these herbal drugs can help in the prevention and treatment of obesity by inducing inhibition or reduction of weight gain.

In the composition of the present invention, preferably from 1.0 to 30.0 parts by weight, 1.0 to 20.0 parts by weight of the upper leaves, 1.0 to 20.0 parts by weight of the leaves, 1.0 to 20.0 parts by weight of the leaves, neck and 1.0 to 30.0 parts by weight, gold and silver (Lonicera Japonica Thumb ) 1.0 to 20.0 parts by weight.

Pine needles (Pinus densiflora SIEBOLD et ZUCCARINI) are pine leaves, have been described as reassuring and not hungry, and are known to reduce arteriosclerosis and to suppress obesity.

Upper leaf (桑葉 or upper limb) (Morus albaL.) Is a mulberry leaf, has a breeze, blue fever, nominal efficacy, heat-fever, headache, purpose, dry mouth. Moracetin, rutin, quercetin, ecdysterone isoquercetin, inosterone, etc., and the mulberry leaf extract contains anti-obesity and hypoglycemic It has an effect.

The main components of birch leaf (Betula Platyphylla Sukatvar.japonica Hara) are Trierpenoid betulin, behenic acid, etc., and it can be found in Chinese medicine dictionary, which is effective in nourishing tonic and skin diseases.

In juniper (Juniperus Chinensis) extracts, Udercyleric aicd and cedrolic acid were found to be higher than those found in other extracts.

The flowering components of Chaenomeles Sinensis Koeln are found to contain pelrgonidin, cyanin, idaein, chrysanthemin, callisephin, etc., and they are effective in vomiting, blood, and pulmonary tuberculosis.

Gold Silver Coin (Lonicera Japonica Thumb) is mainly composed of Luteolin inositol Iuteolin, tannin. (cerylalcohol, sterin, arachic, myristic acid, elaidic acid, linolenic acid) It is composed of Ionicerin (Luteolin-7-rhamnoglucoside), and its pharmacological action has antibacterial properties, and it is lab-borne Staphylococcus aureus. ), Hemolytic streptococcus, erythrocytes, etc., which exhibit growth inhibition or bactericidal effect, can be found in Chinese medicine dictionaries.

In the composition of the present invention, preferably, as an auxiliary ingredient in addition to the active ingredient, Lycium chinenseMILLER, Ginseng (Panax ginseng CA Meyer), Ginger (Zingiber officinale Rose), Licorice (Glycyrrhiza Glabra. L), Ginseng (Codonopsis lanceolata) Traut), Lonicera Japonica Thumb, Angelica gigas Nakai, and 1.0 to 10.0 parts by weight, respectively.

Lycium chinenseMILLER is a dried fruit of the Goji berry, known to have interpolation, voicing and nominal action.

Angelica gigasNakai is the root of Angelica gigas, it is known to have uterine function, sedation, analgesic, diuretic, vitamin E deficiency treatment, antibacterial action and hypogonadism.

Licorice (Glycyrrhiza Glabra.L) is a sterol compound, a large amount of vitamins, matairesinoside, pinoresinol glucoside (pinoresinol glucoside), etc. It has the effect of expectorant, antitussive, detoxification, food poisoning, blood It is stated in the agreement.

Ginseng (Panax ginseng C. A. Meyer) contains ginseng alkaloids, which enhance physical strength by activating preservation, viability, sympathetic nervous system, and energy metabolism.

Ginger (Zingiber officinale Rose) has been used in various Chinese medicines for its effects such as dry stomach, analgesic, stroke, vomiting, haemul, hydrangea, and warm middle earth. Also, Ginseng (Codonopsis lanceolata Traut) is used for bowel, tonsillitis, and sore throat. It is used in herbal medicine to show effect.

In the composition of the present invention, the extract may be extracted with water, a hydrophilic organic solvent or a mixed solvent thereof, preferably, hot water extraction or alcohol extraction.

The herbal medicine contained in the composition of the present invention may be used by drying the herbal medicine itself according to a conventional drying method, and drying the dried product into finely divided or powdered forms, using a conventional extraction method such as hot water extraction or an organic solvent. It is also possible to purchase and use extract powders already commercially available. The crude drug used in the composition of the present invention can be used for extraction, either dry, undried, or a mixture of these.

According to another aspect of the present invention, the present invention comprises the steps of chopping pine needles, upper leaves, birch leaves, neck and gold leaf (Lonicera Japonica Thumb); Immersion extraction and ultrasonic extraction in water of 50-70 ° C .; Heating the extract to a temperature of 90-110 ° C. to evaporate it; And cooling the evaporated vapor by using a cooling device.

In the manufacturing method of the present invention, preferably further comprising the step of lyophilizing the cooled solution at minus 35-45 ℃ to prepare a herbal dry powder, more preferably by suspending the herbal dry powder in distilled water It characterized in that it further comprises the step of preparing a herbal extract suspension.

Specifically, the extract contained in the composition of the present invention, after cutting the herbal medicine, hot water extraction, cold leaching extraction for about 24 hours to 10 days at a temperature of 60 ℃ with water or alcohol of about 10 to 20 times the weight of the dry slices After the ultrasonic extraction, and secondly heated for about 4 hours at a temperature of 100 ℃ can be prepared by the method of gathering the vapor evaporated to quench using a cooling device.

The present invention also provides a method for preventing and treating obesity comprising administering a composition of the present invention.

In the embodiment of the present invention, the herbal extract composition was compared with Oristet, which is a medicine, and a comparative animal experiment was performed. The composition of the present invention exhibits a weight increase inhibitory effect when the rats are fed a high fat component for 6 weeks. In addition, the clinical trial showed a significant decrease in body weight, body fat percentage, abdominal visceral fat reduction, and did not change the arteriosclerosis index.

The composition of the present invention may be prepared by a method known in the pharmaceutical field for use as a therapeutic agent, and may be mixed with a pharmaceutically acceptable carrier, excipient, diluent, etc. to powder, granules, tablets, capsules, drinks, Or in the form of an injection or the like. They can also be administered orally or parenterally.

The dosage of the pharmaceutical composition comprising the herbal extract as an active ingredient in the present invention may be appropriately selected according to the age, sex, condition and symptoms of the disease of the patient, and preferably administered so that the daily intake of adult is 1 to 3000 mg. It is suitable.

Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

Example 1 Preparation of Compositions of the Invention

(1) Method of Preparation Composition A

17 parts by weight of pine needles, 17 parts by weight of upper leaves, 10 parts by weight of birch leaves, 10 parts by weight of neck and head, 10 parts by weight of Lonicera Japonica Thumb, 6 parts by weight of Lycium chinenseMILLER, 8 parts by weight of Panax ginseng CA Meyer , 8 parts by weight of ginger (Zingiber officinale Rose), 6 parts by weight of licorice (Glycyrrhiza Glabra. L), 6 parts by weight of ginseng (Codonopsis lanceolata Traut), 2 parts by weight of Angelica gigasNakai, and then 20 times of the dried section After immersion in water, the temperature is extracted by hot water extraction and ultrasonic extraction at 60 ° C for about 72 hours, and then secondly heated at 100 ° C for about 4 hours, and the vaporized vapor is quenched by using a cooling device. Rapid freezing at 15 hours, and vacuum-dried at least 35 hours under a pressure of 0.23 mHg or less to obtain a powder (FIG. 1). A solution (5% / 10% suspension) suspended in 100 mL of distilled water, 5 g / 10 g of dry powder, was prepared before administration and used in animal and clinical experiments.

(2) Preparation of Composition B

22 parts by weight of pine needles, 22 parts by weight of upper leaves, 10 parts by weight of Lycium chinenseMILLER, 11 parts by weight of ginseng (Panax ginseng CA Meyer), except for cotyledon, neck and gold and silver, which were first identified in the present invention in composition A , 11 parts by weight of ginger (Zingiber officinale Rose), 10 parts by weight of licorice (Glycyrrhiza Glabra. L), 10 parts by weight of ginseng (Codonopsis lanceolata Traut), 4 parts by weight of Angelica gigasNakai, and then 20 times of the dried section After immersion in water, the temperature is extracted by hot water extraction and ultrasonic extraction at 60 ° C for about 72 hours, and then secondly heated at 100 ° C for about 4 hours, and the vaporized vapor is quenched by using a cooling device. Rapid freezing at 15 hours, and vacuum-dried at least 35 hours under a pressure of 0.23 mHg or less to obtain a powder (FIG. 1). A solution B (5% / 10% suspension B) suspended in 100 mL of distilled water in 5 g / 10 g of dry powder was prepared prior to administration and used in animal and clinical experiments.

Example 2: Weight Gain Suppression Experiment

The experimental animals were purchased from Daehan Biolink and adapted to normal diet for 1 week, and 5 weeks old C57BL / 6J male mice were used as experimental animals. After one week, mice with an average weight of 5% or less were excluded. In addition, the diet was similar to the diet of obese people who enjoy high fat and high calorie intake for 2 weeks to be adapted to food. Mice below 5% of average body weight were excluded from the experiment.

A group of 10 mice were orally administered 1 mg to 100 mg / Kg of Oristat (Xenical), a drug approved for weight loss by the FDA, for 6 weeks between 2 pm and 4 pm daily. 0.5 mL of the 5% suspension prepared in Example 1 and 0.5 mL of the 10% suspension were administered orally for 6 weeks at the same time, and the effect of inhibiting weight gain was tested, and as a comparison group, 0.5 mL of saline solution was orally administered for 6 weeks. Was used.

TABLE 1 Weight gain inhibition experiment in mice

Experimental group Weight before experiment Body weight after 6 weeks Remarks Control (saline) 26.1 ± 0.2 29.1 ± 0.4 Orlistat (1mg / Kg) 26.0 ± 0.4 29.3 ± 0.6 Orlistat (30mg / Kg) 26.0 ± 0.3 27.4 ± 0.4 Orlistat (100mg / Kg) 25.9 ± 0.2 26.1 ± 0.3 5% Suspension A (0.5 mL) 26.0 ± 0.2 25.8 ± 0.3 10% Suspension A (0.5 mL) 26.0 ± 0.3 26.2 ± 0.5 5% Suspension B (0.5 mL) 26.0 ± 0.2 28.0 ± 0.4 10% suspension B (0.5 mL) 26.1 ± 0.3 27.8 ± 0.4

Mean ± standard deviation

5% / 10% herbal dry powder suspension A showed the same level of weight control effect as Orlistat, a drug in mice fed high fat, high calorie feed (FIG. 2).

Example 3: Evaluation of weight inhibition clinical efficacy

In animal studies, clinical efficacy evaluations were performed in humans, showing the results of weight-inhibiting effects. The goal of functional foods that indicate the suppression or reduction of obesity is to gain health benefits by taking them for a certain period of time, which is a key part of the overall study. The evaluation of efficacy of the clinical trial was conducted with reference to the registered patent. (Korean Patent 10-0504124) The general characteristics of the subjects who participated in the clinical trial are shown in Table 2.

[Table 2] General characteristics of clinical trial participants

Distribution female male Age (years) 40.81 ± 5. 34 39.12 ± 5. 48 43.36 ± 5.26 Composition ratio 20 12 (60%) 8 (40%)

100 mL of the 10% suspension A prepared in Example 1 was administered orally once a day for 12 weeks. After 12 or 13 weeks, the abdominal fat mass, fat percentage, muscle mass, hip circumference and subcutaneous fat thickness were measured and compared with those before taking. The results are shown in Table 3 below. The summary of the clinical trial results is as follows. Obesity-related changes in body values are shown in Tables 5 and 3 to 4 below.

TABLE 3 Significant Body Index Changes after Taking 10% Suspension A

Metric Before taking After taking Body fat mass (Kg) 28.74 ± 6.73 26.39 ± 6.23 Body fat percentage (%) 39.56 ± 5.88 35.78 ± 5.67 Muscle mass (Kg) 40.45 ± 6.26 41.43 ± 6.02 Hip circumference (cm) 104.35 ± 6.34 102.18 ± 4.84 Subcutaneous fat douche (mm) 32.78 ± 8.67 26.56 ± 5.34

Mean ± Standard Deviation

The 10% suspension significantly reduced body fat by 2.5 kg and body fat percentage by 3.8%. In contrast, muscle mass was significantly increased by 1.00 kg. In addition, the subcutaneous fat thickness was decreased by 6.22 mm and hip size by 2.17 cm, indicating a significant effect on the reduction of obesity rate.

TABLE 4 Measures of Health Indicators after Taking 10% Suspension A

Measurement item Before taking After taking Top blood pressure 126.50 ± 2.50 125.30 ± 3.60 Lowest blood pressure 79.60 ± 2.00 80.10 ± 2.40 Pulse (times / minute) 73.56 ± 2.58 73.46 ± 2.30 Blood sugar (mg / dl) 104.5 ± 6.44 105.50 ± 8. 47 Total cholesterol (g / dl) 218.67 ± 10.58 182.52 ± 12.80 GPT (U / L) 14.51 ± 1.70 14.73 ± 2.20

Mean ± standard deviation

Significant improvement in constipation was observed during the treatment period, and none of the patients reported any special side effects. The measurements of other health indicators before and after dosing are shown in the table. The composition did not affect health indicators such as blood pressure, pulse rate and blood sugar. It also did not affect liver function (GPT). Therefore, the composition does not affect the general health indicators, and there are no side effects. In addition, blood lipid analysis showed a significant decrease in blood cholesterol.

As described above, the composition of the present invention is formulated in the form of a functional food or pharmaceutical composition, such as dry powder or suspension, so that the weight gain inhibition or reduction is excellent to the same level as the FDA-approved drug without side effects, It can be effectively used for treatment.

Claims (7)

A composition for the prevention and treatment of obesity, comprising extracts of pine needles, upper leaves, birch leaves, throats, and Lonicera Japonica Thumb as active ingredients. The method according to claim 1, which contains 1.0 to 30.0 parts by weight of pine needles, 1.0 to 20.0 parts by weight of upper leaves, 1.0 to 20.0 parts by weight of birch leaves, 1.0 to 30.0 parts by weight of trees and leaves, and 1.0 to 20.0 parts by weight of Lonicera Japonica Thumb. Characterized in that the composition. According to claim 2, Lycium chinenseMILLER, Ginseng (Panax ginseng CA Meyer), Ginger (Zingiber officinale Rose), Glycyrrhiza Glabra. L, Ginseng (Codonopsis lanceolata Traut), Lonicera Japonica Thumb ), Angelica gigas (Nagas) each of the composition characterized in that it further comprises 1.0 to 10.0 parts by weight. The composition of claim 1, wherein the extract is hot water extracted or alcohol extracted. Chopping the pine needles, upper lobe, birch, neck and Lonicera Japonica Thumb; Immersion extraction and ultrasonic extraction in water of 50-70 ° C .; Evaporating the extract to a temperature of 90-110 ° C .; And cooling the evaporated vapor by using a cooling device. 6. The method of claim 5, further comprising the step of lyophilizing the cooled solution at minus 35-45 ° C. to produce a herbal dry powder.  7. The method of claim 6, further comprising suspending the herbal dry powder in distilled water to prepare a herbal extract suspension.

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