KR101824016B1 - Pharmaceutical composition for preventing or treating arthritis comprising extract of Taraxacum platycarpum H. Dahlsi, Saururus chinensis Baill, Lonicera japonica Thunb, Commiphora myrrha Engl., Clematis manshurica Rupr, Alisma orientalis (Sam) Juzep, Akebia quinata Decne, Plantago asiatica L, Ulmus pumila L., Phyllostachys nigra var. henonis (Bean.) Stapf, Cassia tora L, Angelica gigas Nakai, Paeonia lactiflora Pallas and Glycyrrhiza uralensis Fisch. as an active ingradient - Google Patents

Abstract

The present invention relates to a pharmaceutical composition or a health functional food for preventing or treating arthritis comprising a mixed extract of Taraxacum platycarpum H. Dahlst, Saururus chinensis baill, Lonicera japonica thunb, Commiphora myrrha engl., Clematis mandshurica rupr, Alisma orientalis (sam) juzep, Akebia quinata decne, Plantago asiatica l, Ulmus pumila l., Phyllostachys nigra var. henonis (bean.) stapf, Cassia tora l, Angelica gigas nakai, Paeonia lactiflora pallas and Glycyrrhiza uralensis fisch. The composition comprising the mixed extract of Taraxacum platycarpum H. Dahlst, Saururus chinensis baill, Lonicera japonica thunb, Commiphora myrrha engl., Clematis mandshurica rupr, Alisma orientalis (sam) juzep, Akebia quinata decne, Plantago asiatica l, Ulmus pumila l., Phyllostachys nigra var. henonis (bean.) stapf, Cassia tora l, Angelica gigas nakai, Paeonia lactiflora pallas and Glycyrrhiza uralensis fisch can be useful for preventing or treating arthritis by reducing pain and improving mobility of the joints of patients who suffer from arthritis.

Description

TECHNICAL FIELD The present invention relates to a pharmaceutical composition for preventing or treating osteoarthritis, which comprises a mixed extract of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Gingkohwa, Myrrh, Gemongrass, Pharmacopoeia, Phyllostachys nigra var. pomila L., Ulmus quinata decana, Plantago asiatica L, Ulmus pumila L., Lycoris japonica Thunb, Commiphora myrrha Engl., Clematis manshurica rupr, Alisma orientalis (Sam) var. . henonis (Bean.) Stapf, Cassia tora L, Angelica gigas Nakai, Paeonia lactiflora Pallas and Glycyrrhiza uralensis Fisch. as an active ingradient}

The present invention relates to a pharmaceutical composition for the prevention or treatment of arthritis, which comprises a mixed extract of Porphyrae, Staphylococcus aureus, Mulberry, Myrrh, Gamshin, Pharmacia, .

Degenerative arthritis, a typical osteoarthritis disease, is a chronic arthritis disease characterized by symptoms such as arthritis, edema and redness that last for more than two weeks, malacia and pain. Arthritis can be classified into at least 100 forms, but the most frequent occurrence is osteoarthritis and rheumatoid arthritis, which account for about 60% of all osteoarthritis, also known as degenerative arthritis, , Which causes severe pain and stiffness of joints due to destruction of cartilage surrounding bone due to aging of joints. In particular, osteoarthritis is a degenerative disease that occurs in the older age group with decreased metabolism and cell regeneration ability, and its fundamental treatment is almost impossible, and surgical operation, drug therapy and physical therapy are performed. However, The drug is difficult to cure, and it causes systemic side effects such as digestive disorder, gastrointestinal tract disorder and kidney function decrease. As the age of the patient increases, the incidence of side effects increases. Therefore, in the treatment of many osteoarthritis diseases, Administration has many problems. Therefore, we are interested in articular cartilage protection, prevention of cartilage degradation, and promotion of regeneration. We are also interested in artisolytic enzyme inhibitors, free radical scavengers (active oxygen elimination enzymes such as SOD) (Chondroitin, Glucosamine, etc.) have been actively studied.

Another major arthritis, rheumatoid arthritis, is chronic inflammation of the synovial membrane of the joints or tendons, resulting in synovial proliferation and increased synovial fluid resulting in joint edema and pain. Rheumatoid arthritis is a systemic inflammatory reaction that affects many tissues and organs (skin, blood vessels, heart lungs, muscles) in the body and especially affects the joints causing irreversible proliferative synovitis, It goes on rigidly. Although the cause of rheumatoid arthritis has not been elucidated yet, it is known that autoimmune reactions play an important role in the chronicity and progression of this disease. That is, T cells play an important role in the local release of inflammatory mediators and cytokines, resulting in a continuous autoimmune reaction that destroys joints. The main symptoms that appear are fatigue, helplessness, pain, arthritis As it progresses, it is accompanied by fever and physical weakness. In addition, muscle atrophy and muscle spasm are known to affect movement of the joints in the vicinity of inflamed joints. (Badger, A. M., Cook, M. N., et al., Journal of Pharmacology and Experimental Therapeutics, 1999, 290: 587-593)

In Korea and other Asian countries such as China and Japan, the traditional medical system of Oriental medicine has been equipped with knowledge gained through thousands of years of clinical evaluation. Therapeutic drugs that have proven efficacy for a long time have been used, And is being utilized.

In Oriental medicine, arthritis is mainly expressed as 虚证 (證证), the clinical expression of non ization (證证) is mainly pain, pain may be pathologically caused by the problem of blood circulation. Major external factors can be classified into wind, cold, swelling, fever, etc. These causes cause the circulatory disturbance of joints and muscles and cause pain. In severe cases, joints Limitations of range of motion appear. Internal factors are the main causes of liver and kidney in the liver (臟 腑). Muscle is the liver, liver is the liver, liver is the liver, To nourish the liver functioning to regulate the blood of the liver (瘀血) can prevent pathological products. In addition, God controls the bones (kidney bone) and controls the body's sperm, which serves to nourish the bone marrow and to infect the skeleton. Therefore, joint anomalies are closely related to the function of liver and kidney, and the prevention and treatment of osteoarthritis in oriental medicine is based on this principle, aiming at more fundamental treatment.

Poe-Young (蒲 公英, Taraxacum platycarpum H. Dahlsi; Dandelion) is a herb of Asteraceae. It is also known as 鳧 公 英, 공 公 草, 公 公 英, and 黄花 地 丁. In Oriental medicine, chewing detoxification (热热 detoxification, treatment by the way to remove the poison), incarceration (肿 肿 散结, 癰 疽) And diuretic gonorrhea (尿尿 通 息; diuretic (尿尿 (尿尿 (尿尿) and the urine is a well-known effect) is known to have the effect of jaundice, sore throat, janggyeong (肠 癰; And is used in the treatment of Onion.

Saururus chinensis Baill) is a herb of Saururus chinensis , which is also known as 菜 (菜), 背 어 (紫 背 魚 草), 저 소 소 (臭 猪 巢), and 中 药 (重 药). In oriental medicine, the effect of cleansing detoxification (cleansing heat), exfoliation (exfoliation) and eliminating the poison from the abdominal 尿 () It is known that it has the efficacy of fever and fever, and fever is feverish in fever (痰 热 喘虚; fever with breathing and coughing) and fever (fever, fever) Symptoms that occur) are used to treat.

Honeysuckle (金銀花, Lonicera japonica Thunb.) Is honeysuckle flower with herbs and honeysuckle (忍冬花), silver (銀花), it is possible medicinal flowers bulriwoomyeo also labor Tuesday (鷺花). In Oriental medicine, there is a method of eliminating diarrhea by eliminating cheongryeol detoxification (淸 热 毒毒) and 肝血 尿 (血血 尿; blood 热 邪) and 散 散风 热It is known that it has the efficacy such as relieving the fever (wind heat)), and it is known to have the efficacy such as 丹毒 (symptom of fever as the skin of the affected part is burned up due to infection etc.) (Cold fever caused by taking wind) and chest pains (heat poisoning (heat poisoning) into the liver (肝 经) and the inside of the neck is blocked) is used to treat.

Myrrh (没 药, Commiphora MyRrha Engl .; Myrrh) is a herb of olive oil, also called herbal medicine, and it is possible to use resin. In Oriental medicine, there is an effect of eliminating the scattered 气, the effect of removing blood from the blood, and the elimination of the umbilical cord blood. It is known to have the efficacy of relieving pain and relieving swelling and relieving swelling of the wound. It is also known to be effective for the treatment of menstrual cramps, , Gingko muscle (bone scrape), vaginal damage (injury caused by falling or bumped), cardiovascular disease (heart abduction pain; Etc.), and 癰 疽 (气血) is blocked by poison 邪 (blocking), between the 肉肉 (肉肉) and bone (肿 tumor), etc.) Is used in the treatment of.

Clematis manshurica Rupr. Is a medicinal herb of the buttercups. It is the herb of the buttercups, which is called Rongsu, Risso, Hyosen, Bokseongjeonseon, It is also known as the iron wire) and it is possible to medicinally use roots. In Oriental medicine, dehumidification of giant wind (风风 除濕; 祛濕 (邪 ((邪 邪) and stay in the body to treat the symptoms of pain to move), 行 Ki (行 how to make good circulation and passageway It is known to have the efficacy of communicating lacock and stopping the pain), and it is known that after bone cementing (such as bone or throat, it is caught in the neck or in the esophagus or airway) , Limb 麻木 (a symptom in which the sensory functions of the limbs and body are not exercised properly), muscle palsy (muscle pains in the limbs) And customary vigor (风 濕 痛痛; due to customs and sickness) is used to treat.

Alasma orientalis (Sam) Juzep; Is a medicinal herb of apple, and it is also called hydrangea, mulberry (泽 芝), sudden death (泻 泻), and astringent (天)). In oriental medicine, it is the effect of removing the residue that has submerged in the uterus or bladder, such as 祛 胞 中 留 垢; ), The 胱 胱 화 ((bladder; (((((((((((((((((((((((((((((((((((광 광 광 광 초 초 초 초) It is known that it has the efficacy such as the water sucking water (the effect of extracting the water 气 in three seconds), and it is known to have the efficacy of the phlegm sensation (痰 飮; Diarrhea, urine, urine, urine, urine, urine, urine, urine, urinary incontinence, urinary incontinence, urinary incontinence, And a water gulf alone (水 肿 滿 滿; And the like.

Akebia quinata Decne .; Is a medicinal herb that is also called medicinal herb. It is also known as Tongchou, Jing, 富 藤, Manchu, and Shiraki. In oriental medicine, there is a tendency to deepen the river (deepening effect, deepening effect), iso ssang (小便; efficacy to urinate), blue wasted heat (淸 肺热, cooling effect of waste heat) It is known that it has efficacy such as blood circulation, blood circulation, blood circulation, blood circulation, blood circulation, blood circulation and blood circulation. (身 身 痛; 身 身 身 이 이 이 이 이 이 이 이 이 이 이 이 이) And that systemic edema occurs as a result of stagnation).

Cha Electronics Co., Ltd. asiatica L .; Plantain) is a medicinal herb of plantainaceae. It is also known as a carpenter 's room and a hippopotamus (穂 衣 子), and seeds can be medicated. In Oriental medicine, it is possible to eliminate the cause of phlegm, such as phlegm (phlegm), phlegm (phlegm), sodam (phlegm), and jungefum (phlegm) It is known that it has efficacy such as efficacy of Lee Soo-sam (efficacy) and efficacy of herbal medicine (herbal medicine) and cheongryeo (cooling effect of cold or cold medicine) (痰 热 咳泻; the heat in the body (痰) with the fire (痰热 证) is a result of coughing (痰 热热 证) cough out), intestinal (眼血 内; (A disease in the eye), urine discomfort (discomfort), and blood leukaemyeo (肿血 血; blood vomiting blood and vomiting) is used to treat.

Ulmus (楡 root skin, Ulmus) pumila L .; Is a medicinal herb of elm, which is also known as milky white bark, bamboo bark, yuwu tree, and udon bark. It can also bark bark and root bark. In oriental medicine, there is a method to be used when the appearance of gonorrhea occurs due to Ishimontom (haechoongryeon), goddam (phlegm), and detoxification (extinction) It is known to have an efficacy such as the effect of cutting off the skin of the skin and improving the quality of the skin. It is known that scabies are a scab in the skin, (A symptom of lump in the lymph nodes in modern times), urine discomfort (discomfort and discomfort), and fainting (discomfort)淋;; wet heat (热热) and 痰 (痰濁) flows into the bladder (bladder) symptoms are used to treat.

Phyllostachys There is nigra . henonis (Bean.) Stapf; Cotton or dough) is a medicinal herb of rice paddy field. It is also called bamboo leaf (bamboo leaf) and bamboo leaf core (bamboo leaf core). In Oriental medicine, it is known that the effect of eliminating the cold blood (淸 血; blood 熱 을 을), 淸 热 除 了 (the effect of controlling the symptoms of chest tightness by cooling the heat) It is known to have sputum, sputum, and urinary diuresis, and it is known that it has the effect of making urine out of the mouth. (Urine short red, urine does not come out cool, red, small amount), pediatric span (小儿 驚癎; blood bloom, (A kind of epilepsy in childhood) and area (redness of face).

Cassia tora L. is a medicinal herb of soybeans. It is a herb with herbaceous herbs, 羊 明, 羊明, maje liquor, 綠 綠, It is also known as 子 子, 草 决 明子, and seeds are medicinal. In Oriental medicine, the name of the company (泻 肝 明目; the effect of brightening the eyes to light the treatment of the effect), Yunchunshan (腸肠 通;; bowel (bowel) It is known to have the efficacy of blue nematode (淸 肝 明目; the effect of cooling the liver and clearing the eyes), and it is known to have secretion of stool (constipation, constipation), dusk (head wind, It is used for the treatment such as the purpose (eye redness, symptom of congestion of eye whiteness), repetition (腹;;

Angelica gigas Nakai ( Angelica gigas Nakai) is a medicinal herb that is also known as lily of the valerian and can be used for medicinal purposes. In oriental medicine, it is the effect of treating the lack of blood or blood by seeing the blood of blood (补血 和 血, blood), the effect of wetting the dry area and smoothing the intestine It is known to have the efficacy of landscape gardening (经经 止痛, harmonizing the menstrual cycle and stopping the pain), and it is known to have the efficacies of circulatory abdominal pain (stomach pain without menstrual bleeding), menstrual relief (Difficulty in seeing the stool due to lack of intestinal fluid), dizziness (dizziness), and vaginal damage (stomach palsy) And damage caused by falling or bumping).

Paeonia lactiflora Pallas) is a medicinal herb of buttercups, also known as white peony, gold peony, and can be used for medicinal purposes. In Oriental medicine, one of the 淸 肝 (淸 肝 청 清 法), a method of washing the hepatome that is excessively vigorous by using the cold and cold medicinal substance of the writing nature, 阴 阴 (阴气) 영 胱 熱 熱 熱 熱 화 화 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 장 (Hepatopathy 肝痛; 肝气 (肝 气) is an effective treatment to relieve the pain and relieve the sudden aura) The pain is caused by the pain of both sides and the sides are sore. The pain is aching, the chest is achy, the lie is not straight, the muscle is stiff, the legs and waist are heavy, etc.), and the menstrual pain (经oli 痛经; ), Endometriosis (oculomatosis; bleeding in the whites of the eyes) And it is used for the treatment of hemorrhoids (a symptom of gonorrhea, a symptom of mixed urine and sick blood).

Licorice (licorice, Glycyrrhiza uralensis Fisch.) is a medicinal herb of soybean which is a herb of Manchurian licorice, MICO, citrus,, 甛 草, 蜜 草, Yeongtong, It is also known as powder grass, and it is possible to medicinally use roots. In oriental medicine, there is a tendency to increase the strength of the muscles and muscles in the muscles of the muscles and muscles of the muscles and muscles of the muscles and muscles of the muscles and muscles of the muscles, (益 精; 结 精 (精 气) to benefit the beneficiary), the termination of the cold (发汗) 发汗 (氣气) It is known to have efficacy such as harmonious pharmacy (harmonious 药; harmonizing all medicines) and sea bean poison (efficacy of detoxifying many medicines) 찰 UL is a condition in which the muscles of the limbs are stiff and unsteady due to seizures, usually due to a lack of blood (insufficiency of blood), the infestation of wet heat (寒血 濕 熱) ), The earth (the finger of the fingertip, swelling of the finger or the toe, infected, poisoning, and inflammation)渴 渴 渴 渴 渴 다음 다음 다음 다음 다음 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 병 ), Hemorrhage (urea blood; a mixture of blood in the urine), and lungs (lung;

Accordingly, the present inventors have found that the mixed extracts including all of the above extracts, such as the above-mentioned extracts, such as Porphyrae, Rhododendron, Phragmites, Myrrh, Goryeon, Phataceae, , And confirming the remarkable improvement of arthritis, thereby completing the present invention.

The present invention relates to a pharmaceutical composition for the prevention or treatment of arthritis, which comprises a mixed extract of Porphyrae, Staphylococcus aureus, Mulberry, Myrrh, Gamshin, Pharmacia, .

In order to achieve the above object,

Poe-Young (蒲 公英, Taraxacum platycarpum H. Dahlsi), Houttuynia cordata (魚腥草, Saururus chinensis Baill), honeysuckle (金銀花, Lonicera japonica Thunb.) , myrrh (沒藥, Commiphora myrrha Engl.), Clematis manshurica Rupr., Taesa (瀉泻; Alisma orientalis (Sam) Juzep), japon (木 通, Akebia quinata Decne.), secondary electron (車前子, Plantago asiatica L.), yuukeeepi (楡 root skin, Ulmus pumila L.), jukyeop (竹葉, Phyllostachys nigra var henonis ( Bean..) Stapf), Cassia tora (決明子, Cassia tora L.) , Angelica (當歸, Angelica gigas Nakai), peony (芍藥; Paeonia lactiflora Pallas) and licorice (licorice, Glycyrrhiza The present invention provides a pharmaceutical composition for preventing or treating arthritis, which comprises a mixed extract of Lactobacillus sp. uralensis Fisch. as an active ingredient.

In addition, the present invention provides a health functional food for improving arthritis containing the extract as an active ingredient.

The composition comprising the mixed extract of Poeongyong, Gosengcho, Ginseng, Myrrh, Gimyunsoon, Pharmacia, Gyokbuk, Chaejung, Yukaepi, Bacillus thuringiensis, Mobility, the composition can be useful for preventing or treating arthritis.

1 is a graph comparing the cytotoxicity of the extract of the present invention.
FIG. 2 is a graph comparing the results of the articular protease inhibition experiments of the extract according to the present invention.
3 is a graph comparing the effects of the extract of the present invention on arthritis improvement in mice.
Figure 4 is a graph comparing the effects of the extract of the present invention on arthritis improvement in a recipient.
FIG. 5 is a graph showing the effect of improving the arthritis of a person taking an extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The invention pogongyoung (蒲公英, Taraxacum platycarpum H. Dahlsi) , Houttuynia cordata (魚腥草, Saururus chinensis Baill), gold silver flower ( Lonicera japonica Thunb.), myrrh (没 药, Commiphora myrrha Engl.), Clematis manshurica Rupr., Taesa (瀉泻; Alisma orientalis (Sam) Juzep), japon (木 通, Akebia quinata Decne.), secondary electron (車前子, Plantago asiatica L.), yuukeeepi (楡 root skin, Ulmus pumila L.), Seokbyeo (竹葉, Phyllostachys There is nigra . henonis (Bean.) Stapf, Cassia tora L., Angelica gigas Nakai, Paeonia lactiflora Pallas) and licorice (licorice, Glycyrrhiza The present invention provides a pharmaceutical composition for preventing or treating arthritis, which comprises a mixed extract of Lactobacillus sp. uralensis Fisch. as an active ingredient.

The extract is preferably, but not exclusively, prepared by a process comprising the steps of:

(1) drying each of the four or more active ingredients to remove moisture, and then pulverizing the dried powder;

2) Extracting at a room temperature an extracting solvent in the dried step 4) of step 1), adding the extracting solvent to the dried seaweed, gangbyeol, gyeonghwa, myrrh, gyeongseon, And

3) filtering the extract of step 2) and concentrating the filtrate under reduced pressure.

In the above method, it is possible to use without limitation such as those cultivated on the market or commercially available, such as Pongyeonggwang, Hwasungcho, Gwanghwaseong, Myrrh, Goryeon, Pharmacopoeia,

In this method, drying of step 1) may be, but not limited to, vacuum drying, vacuum drying, boiling drying, spray drying or lyophilization.

In this method, the pulverization of step 1) may be, but not limited to, those obtained by mechanical pulverization, freeze-drying pulverization or air stream pulverization.

In the above method, the extraction of step 1) may be carried out by adding extraction solvent to each of the dried pufoil, obtusa, gilt eum, myrrh, gestational line, pharmacopoeia, It can be extracted by adding an extracting solvent after mixing the four kinds of ginseng, ginseng, ginseng, myrrh, goryeon, phytase, ginseng, tea ginseng,

In the above method, the extraction method of step 2) may be a conventional method in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, and is extracted once to five times by a hot water extraction method And may be, but is not limited to, repeating extraction three times more specifically. The extraction solvent may be added to the dried sample in an amount of 0.1 to 10 times, preferably 10 times. It is preferable that the extraction is carried out at 70 to 110 DEG C in hot water extraction. When extracted at a temperature of less than 70 캜 at the time of extraction, the dissolution rate of the active ingredient contained in the herbal medicine is low, and when the temperature exceeds 110 캜, there is a problem that the active ingredient is destroyed. More preferably 85 to 105 ° C, and further preferably 93 to 103 ° C. The extraction time is preferably 0.5 to 7 hours. If the extraction time is less than 0.5 hour, the effective ingredient contained in the herbal medicine is not sufficiently extracted. If the extraction time exceeds 7 hours, the efficiency is lowered because the amount extracted is not much compared to the time and resources required. More preferably 1 to 5 hours, more preferably 1 to 3 hours, but is not limited thereto.

In this method, the extraction solvent of step 2) may be water, a C ₁ to C ₂ lower alcohol or a mixture thereof, but is not limited thereto.

In the above method, the vacuum concentration of step 3) may be performed using a vacuum decompression concentrator or a vacuum rotary evaporator, but is not limited thereto.

The arthritis is a disease in which inflammation or cartilage breakage occurs in the synovial membrane surrounding the joint, and may include, but is not limited to, degenerative arthritis or rheumatoid arthritis.

The degenerative arthritis, also called osteoarthritis, refers to a condition in which the cartilage protecting the joint is worn out and is the most common arthritic disease. Although the precise cause is unknown, it is known that aging, genetic factors, and environmental factors act, and after a series of processes in which cartilage fragments are broken off by the cartilage fragments that are disrupted by various causes, And the debris is deformed to form a protrusion called an osteophyte at the edge of the bone. In general, degenerative changes of joints begin at about age 40, and at age 60, about 50% of degenerative arthritis symptoms are known to occur. Proteolytic enzymes, cytokines, and nitric oxide (NO) are known to be major contributors to osteoarthritis. The Nuclear transcription factor κB (NF-κB) is a transcription factor for several genes involved in the immune and inflammatory response. The inflammatory cytokines such as TNF-α and IL-6 and the 5-lipoxygenase (5-LO) , cyclooxygenase-2 (COX-2) and matrix metalloproteinases (MMPs), and this metabolic process in cartilage tissue plays an important role in cartilage tissue degeneration.

The cause of rheumatoid arthritis is a chronic chronic arthritis. However, it is presumed that the immune system attacks the body by immune mechanism abnormality. It usually occurs in the hands, feet, knees, wrists, ankles, and hip joints, but it can occur in any joint and can occur simultaneously in multiple joints. It occurs mainly in adults, but it can also occur in various age groups, including children, with swollen joints and stiffness. The swelling of the joints, such as the joints, causes synovium to swell and joint effusion around the joints. When these symptoms persist for months to years, the cartilage or surrounding tissues of the joints are damaged and the joints become bent or hardened. And it is accompanied by pain.

The composition can improve the survival of cartilage cells and inhibit the activity of articular tissue degrading enzymes.

In a specific embodiment of the present invention, the pharmaceutical composition for preventing or treating arthritis including mixed extracts of the above-mentioned Corynebacterium glutamicum, Corynebacterium glutamicum, Myrrhic acid, Phytophthora, Phytophthora, The composition showed no toxicity to cartilage cells in the cytotoxicity test, and improved survival compared to the control group (FIG. 1 and Table 1), showing the effect of inhibiting the activity of articular tissue degrading enzymes (FIG. 2 and Table 2) Inhibited the production of arthritis-related factors (Table 3), and the mice showed an improvement in movement in more than 70% of the arthritis-induced rats (Fig. 3 and Table 5) And joint mobility (Figs. 4 to 6). Specifically, improvements were observed in over 89% of knee and lower back pain patients (FIGS. 5A and 7) and over 91% of shoulder and upper extremity pain patients (FIG. 5B and Table 7). In addition, it was confirmed that the composition according to the example of the present invention does not show side effects (Table 4).

Accordingly, a composition comprising a mixed extract of Porphyrae, Rhododendron, Gingkohwa, Myrrh, Gimyosan, Pharmacopoeia, Ganoderma, Tea, Eugeniae, Seedlings, Cucumber, Angelicae, Peony, and Licorice can be applied for the prevention or treatment of arthritis.

The composition for preventing or treating arthritis according to the present invention, which comprises a mixed extract of Porphyrae, Rhododendron japonica, Ginseng, Myrrh, Goryeon Line, Pharmacopoeia, Of the present invention contains 0.1 to 99.9% by weight of a mixed extract of the present invention as an active ingredient, and the mixed extracts of the present invention are selected from the group consisting of Pohang, Yongsin, Ginseng, Myrrh, Acceptable carriers, excipients or diluents. The pharmaceutically acceptable carrier may be a mixture of saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components. If necessary, an antioxidant, Other conventional additives such as a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into main dosage forms such as aqueous solutions, suspensions, emulsions, powders, tablets, capsules, rings, granules or injection solutions. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA, 19th, 1990), in a suitable manner in the art.

The composition for preventing or treating arthritis according to the present invention, which comprises the mixed extract of Phellinus lanceolata, Pseudomonas spp., Gingko myrrh, Myrrhic acid, Phytophthora brassicae, Phytophthora brassicae, Capsules, powders, granules, suspensions, emulsions, syrups, and other liquid preparations.

Specifically, the composition of the present invention may be administered orally or parenterally. Formulations for oral administration may be in the form of tablets, troche, lozenge, aqueous or predominant suspension, discrete powder or granules, emulsion, hard or Soft capsule, syrup, or elixir. Binders such as lactose, saccharose, sorbitol, mannitol, erythritol, starch, amylopectin, cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, Magnesium stearate, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax. In the case of capsule formulations, in addition to the above-mentioned materials, liquid carriers such as fatty oils may be included. In addition, for the purpose of formulation as a formulation, it is possible to use, in addition to the above-mentioned components, an acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methylcellulose, methylhydroxybenzoate, Talc, magnesium stearate, and mineral oil, but are not limited thereto.

In addition, the pharmaceutical composition for preventing and treating arthritis, which comprises the mixed extract of the present invention of the present invention, such as a combination of the four or more extracts of the present invention, ginseng root, giulia, myrrh, Parenteral administration may be employed. For parenteral administration, subcutaneous injection, intravenous injection, intramuscular injection, or intraperitoneal injection may be preferably selected. For formulation into parenteral administration formulations, the composition of the present invention is mixed with water to prepare a suspension, which is then formulated into unit dosage forms of ampoules or vials.

The composition comprising the mixed extract of the present invention of the present invention is in a pharmacologically effective amount. The composition of the present invention comprises a mixture of the extracts of Phellodendron tumefaciens, Pseudomonas spp., Gingko myrrh, Myrrhic acid, Phytophthora, Pharmacia, In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dosage level will depend on the type of disease, severity, The sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The composition of the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The dose of the active ingredient according to the present invention will consider safety and efficiency when used in the human body, and it is also possible to estimate the amount used in humans from the effective amount determined through animal experiments. Such considerations in determining the effective amount are described, for example, in Hardman and Limbird, eds., Goodman and Gilman ' s Pharmacological Basis of Therapeutics, 10th ed. (2001), Pergamon Press; And E.W. Martin et al., Remington's Pharmaceutical Sciences, 18th ed., (1990), Mack Publishing Co. In the case of oral administration, an amount of 0.0001-500 mg of the composition of the present invention per kg of body weight per day The dosage may be administered once to several times, preferably in an amount of 0.001 to 100 mg, and more preferably 30 to 500 mg / kg. However, the dosage may be varied depending on the route of administration, the severity of the arthritic disease, sex, weight, age, etc., and therefore the dosage is not limited in any way.

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

Compositions of the present invention may also include carriers, diluents, excipients, or a combination of two or more thereof, which are commonly used in biological agents. The pharmaceutically acceptable carrier is not particularly limited as long as the composition is suitable for in vivo delivery, for example, Merck Index, 13th ed., Merck & Sodium chloride, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components may be mixed and used. If necessary, antioxidants, buffers, And other conventional additives may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into main dosage forms such as aqueous solutions, suspensions, emulsions, etc., pills, capsules, granules or tablets. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA, 18th, 1990) in a suitable manner in the art.

The composition of the present invention may further contain one or more active ingredients showing the same or similar functions. The composition of the present invention may contain 0.0001 to 10% by weight, preferably 0.001 to 1% by weight of the above compound, based on the total weight of the composition.

In addition, the present invention provides a health functional food for improving arthritis, which contains, as an active ingredient, a mixed extract of Porphyrae, Rhododendron, Gingkohwa, Myrrh, Goryeon, Taxa, do.

The extract is preferably, but not exclusively, prepared by a process comprising the steps of:

(1) drying each of the four or more active ingredients to remove moisture, and then pulverizing the dried powder;

2) Extracting at a room temperature an extracting solvent in the dried step 4) of step 1), adding the extracting solvent to the dried seaweed, gangbyeol, gyeonghwa, myrrh, gyeongseon, And

3) filtering the extract of step 2) and concentrating the filtrate under reduced pressure.

In the above method, it is possible to use without limitation such as those cultivated on the market or commercially available, such as Pongyeonggwang, Hwasungcho, Gwanghwaseong, Myrrh, Goryeon, Pharmacopoeia,

In this method, drying of step 1) may be, but not limited to, vacuum drying, vacuum drying, boiling drying, spray drying or lyophilization.

In this method, the pulverization of step 1) may be, but not limited to, those obtained by mechanical pulverization, freeze-drying pulverization or air stream pulverization.

In the above method, the extraction of step 1) may be carried out by adding extraction solvent to each of the dried pufoil, obtusa, gilt eum, myrrh, gestational line, pharmacopoeia, It can be extracted by adding an extracting solvent after mixing the four kinds of ginseng, ginseng, ginseng, myrrh, goryeon, phytase, ginseng, tea ginseng,

In the above method, the extraction method of step 2) may be a conventional method in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction, and is extracted once to five times by a hot water extraction method And may be, but is not limited to, repeating extraction three times more specifically. The extraction solvent may be added to the dried sample in an amount of 0.1 to 10 times, preferably 10 times. It is preferable that the extraction is carried out at 70 to 110 DEG C in hot water extraction. When extracted at a temperature of less than 70 캜 at the time of extraction, the dissolution rate of the active ingredient contained in the herbal medicine is low, and when the temperature exceeds 110 캜, there is a problem that the active ingredient is destroyed. More preferably 85 to 105 ° C, and further preferably 93 to 103 ° C. The extraction time is preferably 0.5 to 7 hours. If the extraction time is less than 0.5 hour, the effective ingredient contained in the herbal medicine is not sufficiently extracted. If the extraction time exceeds 7 hours, the efficiency is lowered because the amount extracted is not much compared to the time and resources required. More preferably 1 to 5 hours, more preferably 1 to 3 hours, but is not limited thereto.

In this method, the extraction solvent of step 2) may be water, a C ₁ to C ₂ lower alcohol or a mixture thereof, but is not limited thereto.

In the above method, the vacuum concentration of step 3) may be performed using a vacuum decompression concentrator or a vacuum rotary evaporator, but is not limited thereto.

In a specific embodiment of the present invention, the pharmaceutical composition for preventing or treating arthritis including mixed extracts of the above-mentioned Corynebacterium glutamicum, Corynebacterium glutamicum, Myrrhic acid, Phytophthora, Phytophthora, The composition showed no toxicity to cartilage cells in the cytotoxicity test, and improved survival compared to the control group (FIG. 1 and Table 1), showing the effect of inhibiting the activity of articular tissue degrading enzymes (FIG. 2 and Table 2) (Table 3). In the animal experiment using the mouse, the movement of the arthritis-induced rats was improved (Figs. 3 and 5), and the pain and joint motility (Fig. 4 to Table 6). Specifically, improvements were reported in patients with knee and lower back pain (Figs. 5A and 7) and patients with shoulder and upper extremity pain (Fig. 5B and Table 7). In addition, it was confirmed that the composition according to the example of the present invention does not show side effects (Table 4).

Accordingly, a composition comprising a mixed extract of Porphyrae, Rhodochrosite, Ginseng, Myrrh, Goryeon Line, Pharmacopoeia, Ganoderma lucidum, Tea, Eugeniae, Seedlings, Cucumber, Angelicae, Peony, and Licorice can be applied to health functional foods for improving arthritis.

The health functional food for improving arthritis of the present invention can be prepared by further containing one or more carriers acceptable for food addition.

The health functional food for improving arthritis according to the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, protective colloid thickener, pH adjusting agent, stabilizer, preservative, glycerin, , A carbonating agent used in carbonated drinks, and the like. These components may be used independently or in combination. The health functional food composition of the present invention may be added as it is or may be formulated together with other food or food ingredients, And can be suitably prepared according to a conventional method. Examples of foods to which the health functional food composition of the present invention can be added include dairy products including caramel, meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, , Beverage, tea, drink, alcoholic beverage, and vitamin complex, and includes all the health foods in a conventional sense.

The food may contain various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, Stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks.

The above components can be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention. In addition, the health functional food composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates may be selected from the group consisting of glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, Polysaccharides such as cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate may be selected from the range of 0.01 to 0.04 part by weight, specifically about 0.02 to 0.03 part by weight per 100 parts by weight of the health functional food of the present invention.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

EXAMPLES The following Examples and Experiments are for the purpose of illustrating the present invention, but the present invention is not limited by the following Examples and Experimental Examples.

<Examples>

Example  One. POE , Persimmon, Myrrh , Command line , Tarsa , A thong, Car electronics , U Manufacture of Extracts of Mixed Extracts of Mycorrhizae, Bamboo Leaf, Cucumber, Angelica, Peony, and Licorice

100 g of each of 100 g of each of the ginseng, licorice ginseng, ginseng, myrrh, gyeongseon, texas, gabang, tea, eugenia, The pulverized medicines were extracted with hot water extraction method, and the temperature of hot water was kept at 95 to 100 ° C and extraction was carried out for 3 hours. The extracts were centrifuged at 1500 rpm for 20 minutes and then the supernatant was collected and concentrated at 60-65 ° C using a vacuum evaporator. The concentrated extract was lyophilized at -80 ° C using a freeze dryer to obtain an extract.

<Comparative Example>

Comparative Example  1. Preparation of Horseshoe extract

The extract was obtained by the method of Example 1 above,

Comparative Example  2. Car electronics  Manufacture of extracts containing

100 g of the secondary electrons were obtained by the method of Example 1 above.

Comparative Example  3. Preparation of bamboo shoot extract

100 g of bamboo leaf was obtained by the method of Example 1 above.

Comparative Example  4. Yugi  Manufacture of extracts containing

The extract was obtained by the method of Example 1 above.

Comparative Example  5. Preparation of peony extract

The extract was obtained by the method of Example 1 above.

Comparative Example  6. Preparation of Cercariae Extract

The extract was obtained by the method of Example 1 above.

Comparative Example  7. POE , The production of Hwasungcho

The extracts were obtained by the method of Example 1 above with 100 g of each of the pore,

Comparative Example  8. Myrrh  And Command line  Preparation of extract

The extracts were obtained by the method of Example 1 above for each 100 g of myrrh and moxibustion.

Comparative Example  9. Tarsa , Throat and Car electronics  Preparation of extract

The extract was obtained by the method of Example 1 above with 100 g of each of phytase, thrush and tea extract.

Comparative Example  10. Yugunpi , The production of bamboo leaf and lily extract

The extracts were obtained by the method of Example 1, except that 100 g of each of the yugi,

Comparative Example  11. Preparation of Angelica gigas, Peony root and licorice extract

An extract of Angelica keiskei koidz., Peony root and Licorice root was obtained by the method of Example 1 above.

Comparative Example  However, Yugunpi , Bamboo salt, catechin and peony extract

The extracts were obtained by the method of Example 1, except that 100 g of each of Sophora japonica, Yugaepi, Bamboo salt,

Comparative Example  13. POE , Gold silver, Command line , Tarsa , Thrips, ginseng and licorice extract

The extracts were obtained by the method of Example 1, except that 100 g of each of 100 g of each of the following ingredients was used.

<Experimental Example>

Experimental Example  1. Cytotoxicity of Chinese medicine extract

MTT assay was performed to confirm cytotoxicity of the composition obtained from the above Examples or Comparative Examples.

Specifically, the hind limb knee joint cartilage of the pigs was sectioned, and the cells were separated and the pig chondrocytes cultured in 10% FBS (Fetal Bovine Serum) were dispensed into a 96-well plate at a density of 1 × 10 ⁴ cells / well , And cultured in a 37 ° C 5% CO ₂ incubator for 24 hours to allow the cells to adhere well. After that, the compositions obtained from the Examples and Comparative Examples were dispensed into each well at a concentration of 0.1 mg / ml, 1.0 mg / ml and 5 mg / ml in the experimental group, And cultured in a 5% CO ₂ incubator. Then, 20 μl of each solution was added to each well at a concentration of 5 mg / ml of MTT (3- (4,5-methylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, Sigma) . After incubation for 3 hours at 37 ° C in a 5% CO ₂ incubator, the supernatants containing MTT were discarded and 200 μl of DMSO was added to each well. After shaking for 10 minutes at 37 ° C in a light- The OD value was measured at 570 nm and repeated three times to compare the values of the control group.

As a result, as shown in Fig. 1 and Table 1, the extract according to the example of the present invention did not show cytotoxicity on cartilage cells, and it was confirmed that the survival rate was improved at a dose of 1.0 mg / ml or more.

Cell survival (%) versus control 0.1 mg / ml 1.0 mg / ml 5 mg / ml Example 1 103 123 124 Comparative Example 1 101 108 109 Comparative Example 2 79 82 81 Comparative Example 3 100 106 108 Comparative Example 4 102 104 105 Comparative Example 5 101 104 104 Comparative Example 6 98 101 103 Comparative Example 7 103 117 118 Comparative Example 8 102 115 115 Comparative Example 9 101 106 113 Comparative Example 10 101 104 108 Comparative Example 11 102 103 103 Comparative Example 12 103 114 115 Comparative Example 13 102 116 116

Experimental Example  2. Confirmation of inhibition of articular protease activity by administration of Chinese medicine extract

A comparative experiment was carried out to confirm the inhibition of the activity of hyaluronidase, which is known as articular tissue degrading enzyme, in the composition obtained from the above Examples or Comparative Examples.

Specifically, hyaluronidase was incubated for 20 minutes in an aacetate buffer solution at 37 ° C to activate potassium hyaluronate as a substrate and the composition obtained from the above Examples and Comparative Examples at a concentration of 1.0 mg / ml, and further cultured for 40 minutes. After incubation, NaOH was added to stop the reaction, potassium borate was added, and the mixture was heated to 100 ° C. The absorbance was measured by DMBA (Dimethylbenzanthracene), and the absorbance was compared based on the values of the control group.

As a result, as shown in FIG. 2 and Table 2, the extract according to the example of the present invention showed the greatest inhibitory effect on the activity of articular tissue degrading enzyme

Test concentration (mg / ml) % Inhibition Example 1 1.0 90.9 Comparative Example 1 1.0 84.6 Comparative Example 2 1.0 79.1 Comparative Example 3 1.0 82.3 Comparative Example 4 1.0 85.4 Comparative Example 5 1.0 83.6 Comparative Example 6 1.0 80.4 Comparative Example 7 1.0 84.9 Comparative Example 8 1.0 84.5 Comparative Example 9 1.0 83.6 Comparative Example 10 1.0 82.6 Comparative Example 11 1.0 83.9 Comparative Example 12 1.0 84.8 Comparative Example 13 1.0 84.2

Experimental Example  3. Confirmation of inhibition of nitric oxide and inflammatory cytokines by administration of herb extracts

Comparative experiments were conducted to confirm the inhibitory effect of the composition obtained from the above Examples and Comparative Examples on arthritis-related factors.

1 × 10 ⁵ cells / well of RAW 264.7 cells were placed on a 48 well plate and the composition obtained from the above Examples and Comparative Examples was pretreated for 2 hours and 1 μg / ml of LPS (lipopolysaccharide) was administered to RAW 264.7 cells Lt; / RTI &gt; for 18 hours. To determine the amount of nitric oxide produced, Griess Reagent (0.1% N- (1-naphthyl) -ethylendiaminedihydrochoride and 1% Sulfanilamide diluted with 2.5% H ₃ PO ₄ ) was added to the supernatant of treated RAW 264.7 cells ) Was added and the absorbance was measured at 550 nm after 10 minutes. In order to confirm the amount of produced TNF-a and IL-12 cytokines, the supernatant of treated RAW 264.7 cells was measured by a manufacturer's experimental method of a sandwich ELISA kit (Biolegend, USA). Absorbance was measured at 450 nm using an ELISA microplate reader (Power Wavex340, NIOTEK-INS TRUMENTS, INC) and compared based on the control values.

As a result, as shown in Table 3, the extract according to the example of the present invention showed excellent inhibitory effect on NO, TNF-α and IL-12

Production rate compared to the control (%) NO TNF-a IL-12 Example 1 46.2 58.8 52.9 Comparative Example 1 49.6 66.9 60.2 Comparative Example 2 60.2 106.4 80.9 Comparative Example 3 90.9 82.3 60.7 Comparative Example 4 46.6 83.9 62.4 Comparative Example 5 47.1 83.9 63.0 Comparative Example 6 48.5 86.4 63.7 Comparative Example 7 46.2 74.6 55.6 Comparative Example 8 46.6 75.9 57.8 Comparative Example 9 47.1 82.3 58.5 Comparative Example 10 47.1 83.9 60.7 Comparative Example 11 46.6 84.7 63.7 Comparative Example 12 46.2 76.5 57.0 Comparative Example 13 42.2 88.9 56.5

Experimental Example  4. Identification of mouse toxicity by administration of herb extract

In order to confirm the acute toxicity of the composition obtained from the above Examples or Comparative Examples, a single dose toxicity test was conducted on mice.

Specifically, the compositions obtained from the above-mentioned Examples and Comparative Examples were orally administered to 10 mice per group in an ICR (Institute of Cancer Research) mouse at a dose of 2 g / kg, and the occurrence of deaths was observed.

As a result, as shown in Table 4, the extract according to the example of the present invention has an effect of improving the survival rate of chondrocyte cells and inhibiting the activity of articular tissue-degrading enzymes and showing no fatal side effects

Test concentration (g / kg) Lethality (%) Autopsy result Example 1 2.0 0 None Comparative Example 1 2.0 0 None Comparative Example 2 2.0 0 None Comparative Example 3 2.0 0 None Comparative Example 4 2.0 0 None Comparative Example 5 2.0 0 None Comparative Example 6 2.0 0 None Comparative Example 7 2.0 0 None Comparative Example 8 2.0 0 None Comparative Example 9 2.0 0 None Comparative Example 10 2.0 0 None Comparative Example 11 2.0 0 None Comparative Example 12 2.0 0 None Comparative Example 13 2.0 0 None

Experimental Example  5. Improvement of arthritis in mice due to herbal medicine extract

Animal experiments were conducted to verify the effect of the compositions obtained from the above Examples or Comparative Examples on improving arthritis.

Specifically, STR / ort mice were used, in which arthritis similar to degenerative arthritis (osteoarthritis) of humans was generated by cartilage degradation. The mice of the age of 6 months or more who had a higher incidence of arthritis were brought into the cage and fed with a light-dark cycle at a temperature of 23 ± 3 ° C and a humidity of 50 ± 5% at intervals of 12 hours. Were allowed to freely consume normal feed and water for mice. The subjects who showed the same gait abnormality more than 3 times were selected for the experiment by confirming the individuals who had eruption, edema and morphological changes of the foot and those who tried to move or when they moved when they moved.

The selected individuals were divided into an experimental group (n = 27, each example and a comparative example) and a control group (n = 27). In the experimental group, the extract obtained from the example or the comparative example was orally administered twice a day for 10 weeks , And the control group administered oral water to the same number of times. The degree of symptom improvement after the administration was evaluated as the increase of the movement and the control group was compared.

As a result, as shown in FIG. 3 and Table 5, it was confirmed that the extract according to the example of the present invention exhibited improvement in movement in more than 70% of the individuals, and that the improvement effect of arthritis was superior to the extract of the comparative example.

Improvement in symptoms compared to the control (%) No change or weakness Movement improvement Example 25.9 74.1 Comparative Example 1 40.7 59.3 Comparative Example 2 61.5 38.5 Comparative Example 3 44.4 55.6 Comparative Example 4 44.4 55.6 Comparative Example 5 48.1 51.9 Comparative Example 6 50.0 50.0 Comparative Example 7 40.7 59.3 Comparative Example 8 42.3 57.7 Comparative Example 9 44.4 55.6 Comparative Example 10 46.2 53.8 Comparative Example 11 44.4 55.6 Comparative Example 12 37.0 63.0 Comparative Example 13 38.5 61.5

Experimental Example  6. Arthritis caused by herbal medicine extract In patients  Assess pain and mobility improvement

In order to confirm the therapeutic effect of arthritis on the manufactured composition prepared in Example 1 above, patients suffering from arthritis were examined. From 20 October to 2016, patients aged 20 to 55 years who complained of arthritis problems were divided into three groups according to the composition of the Example and Comparative Examples 7, 12 and 13 for 1 month After 2 doses, the dosage was 110 cc for 30 minutes. The degree of symptom improvement after the administration was assessed as pain relief and improvement of joint mobility.

As a result, as shown in FIG. 4 and Table 6, it was confirmed that more than 30% of pain and joint motility improvement effect was shown in 90% or more patients, and that the extract of the example of the present invention had an improvement in arthritis Respectively.

Improvement of symptoms (%) Less than 10% 10 to 30% 30 to 50% More than 50% Example - 10.0 56.7 33.3 Comparative Example 7 8.3 16.7 20.0 25.0 Comparative Example 12 9.1 18.2 45.5 27.3 Comparative Example 13 8.3 16.7 50.0 25.0

More specifically, as shown in FIG. 5A, the extract according to the example of the present invention showed more than 30% of pain and joint motility improvement effect in 89% or more of knee and lower back pain patients, and as shown in FIG. 5B, More than 91% of the patients complained of pain and joint motility improvement of more than 30% were found to have the effect.

Symptom
Improvement of symptoms Less than 10% 10 to 30% 30 to 50% More than 50% Knee and not
(n = 19) - 2 13 4 Shoulder and upper limb
(n = 11) - One 4 6

<Production Example>

Manufacturing example  1. Preparation of tablets

Using the composition obtained from the above Examples, tablets for oral administration were prepared in the following composition.

[Furtherance]

200 mg of the composition of Example 1, 10 mg of light silicic anhydride, 2 mg of magnesium stearate, 50 mg of microcrystalline cellulose, 25 mg of starch glycolic acid sodium, 113 mg of corn starch,

Manufacturing example  2. Manufacture of Ointment

An ointment preparation was prepared using the composition obtained from the above Example in the following composition.

[Furtherance]

5 g of the composition of Example 1, 20 g of cetyl palmitate, 40 g of cetanol, 40 g of stearyl alcohol, 40 g of myristanisopropyl, 20 g of sorbitan monostearate, 60 g of polysorbate, g, 1 g of methyl parahydroxybenzoate, 1 g of phosphoric acid and purified water

Manufacturing example  3. Preparation of Injection

Using the composition obtained from the above example, an injection was prepared in the following composition.

[Furtherance]

100 mg of the composition of Example 1, 180 mg of mannitol, 25 mg of sodium monohydrogenphosphate, 2974 mg of distilled water for injection

Manufacturing example 4. Health food  Produce

An ointment preparation was prepared using the composition obtained from the above Example in the following composition.

[Furtherance]

1 g of the composition of Example 1, 70 占 퐂 of vitamin A acetate, 1 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, 0.2 占 퐂 of vitamin B12, 10 mg of vitamin C, 10 占 퐂 of biotin, 1.7 mg, folate 50,, calcium pantothenate 0.5 mg, ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25,3 mg, potassium monophosphate 15 mg, dibasic calcium phosphate 55 mg, potassium citrate 90 mg, 100 mg of calcium carbonate, 24.8 mg of magnesium chloride, a mixture of vitamins and a mixture of minerals

Claims (7)

Poe-Young (蒲 公英, Taraxacum platycarpum H. Dahlsi), Houttuynia cordata (魚腥草, Saururus chinensis Baill), honeysuckle (金銀花, Lonicera japonica Thunb.) , myrrh (沒藥, Commiphora myrrha Engl.), Clematis manshurica Rupr., Taesa (瀉泻; Alisma orientalis (Sam) Juzep), japon (木 通, Akebia quinata Decne.), secondary electron (車前子, Plantago asiatica L.), yuukeeepi (楡 root skin, Ulmus pumila L.), Seokbyeo (竹葉, Phyllostachys There is nigra . henonis (Bean.) Stapf, Cassia tora L., Angelica gigas Nakai, Paeonia lactiflora Pallas) and licorice (licorice, Glycyrrhiza A pharmaceutical composition for preventing or treating arthritis, which comprises a mixed extract of uralensis Fisch. as an active ingredient.
The pharmaceutical composition for preventing or treating arthritis according to claim 1, wherein the extract is extracted using water, ethanol or methanol, or a mixture thereof as a solvent.
The pharmaceutical composition for preventing or treating arthritis according to claim 2, wherein the ethanol is a water-soluble alcohol of 30 to 70%.
The pharmaceutical composition for the prevention or treatment of arthritis according to claim 1, wherein the extract is extracted by low pressure high temperature extraction, hot water extraction, reflux extraction, hot water extraction, cold extraction, room temperature extraction, ultrasonic extraction or steam extraction.
1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1 weight ratio.
The pharmaceutical composition for preventing or treating arthritis according to claim 1, wherein the composition is in the form of tablets, liquids, capsules or injections.
A health functional food for improving arthritis, which contains as an active ingredient, a mixed extract of ginseng, ginseng, ginseng, myrrh, gyeongseon, taxa, thistle, tea, eugenia, bamboo leaf,

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