KR102416513B1 - Composition for preventing and improving pet constipation and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a composition for alleviating constipation of companion animals containing Liriope muscari, Rehmannia glutinosa, Adenophora triphylla, Cichorium intybus, Citrus aurantium, Achyranthes japonica, Angelica gigas, etc. More specifically, the present invention relates to a composition for preventing and alleviating constipation of companion animals, further comprising raw materials for increasing the palatability of companion animals and supplying protein in addition to the above herbal medicines. In the present invention, provided is the composition for preventing and alleviating constipation of companion animals, in which, through animal experiments, the digestive tract transit rate test and fecal moisture improvement, which can confirm the substantial improvement in constipation, are specifically confirmed. Through the same, it is possible to significantly alleviate constipation of cats and at the same time minimize the reluctance caused by the smell of herbal medicines to increase palatability, and it is possible to be of great help in relieving stress of cats.

Description

Composition for preventing and improving constipation in companion animals and manufacturing method thereof

The present invention relates to a composition for improving companion animal constipation comprising herbal medicines such as maekmundong, saengjihwang, ginseng, chicory, crustaceans, hyssop, angelicae, and the like. It is effective in preventing and improving pet constipation.

Most pets, including cats, can also experience constipation. There are various causes of constipation, but it occurs particularly frequently in cats due to the nature of cats that do not like water.

The causes of constipation in companion animals are diverse, including diet, environment, pain caused by other diseases, medications, physical obstruction, neuromuscular disease, and endocrine disease.

Among them, the three most common causes can be divided into diet, environment, and constipation caused by pain.

When it comes to diet, poor water intake is the most common cause of constipation. Foods with a lot of bones and other by-products also make the stool hard and cause constipation.

Other than that, an unfamiliar environment may interfere with the pet's comfortable bowel movements and cause constipation. This includes changes in family members, moving, and changes in household arrangement. If you are admitted to the veterinary hospital, you may not have a bowel movement. In the case of a cat, the toilet is not cleaned often, so if it is dirty, it is reluctant to defecate and may cause constipation.

Constipation is a common symptom even in companion animals, and large stools accumulated in the colon press the urethra, making it difficult to urinate. In some cases, a person does not eat and loses weight due to a decrease in appetite, and in the worst case, if there is a problem such as complete loss of function and necrosis of the colon, a part of the colon must be surgically removed.

In order to prevent constipation, adequate intake of dietary fiber and sufficient drinking water are required, but conventionally released food for improving constipation does not show a problem of preference and an effect of improving constipation at the same time, so it is not getting a great response.

Against this background, this technology was developed by paying attention to the fact that continuous research and development is required for a composition for improving constipation that is safe without side effects using only natural raw materials and has a low burden on the body.

Korean Patent Publication No. 10-2020-0141154

An object of the present invention is to select one or more herbal medicines from the group consisting of maekmundong, saengjihwang, ginseng, chicory, crustaceans, hyssop and angelica; and raw materials for enhancing preference and protein selected from the group consisting of fish or meat; to provide a composition for preventing and improving companion animal constipation.

Another object of the present invention is to provide a health supplement composition for preventing and improving constipation in companion animals, including the composition for preventing and improving constipation in companion animals.

Another object of the present invention is to provide a pharmaceutical composition for preventing and treating constipation in companion animals, comprising the composition for preventing and improving constipation in companion animals.

Another object of the present invention is to select one or more herbal medicines from the group consisting of maekmundong, saengjihwang, ginseng, chicory, crust, hyssop and angelica, extracting and freeze-drying; pulverizing one or more from the group consisting of fish or meat; And to provide a method for preparing a composition for preventing and improving companion animal constipation comprising the step of mixing the freeze-dried herbal medicine and the powder.

One aspect of the present invention is a companion animal constipation containing one or more herbal medicines selected from the group consisting of maekmundong, saengjihwang, ginseng, chicory, crust, hyssop and angelica, and at least one selected from the group consisting of fish or meat, and a raw material for enhancing protein. It relates to a composition for prevention and improvement.

In the present invention, the herbal medicine may be used in the form of a powder by directly using the extract of each raw material or by freeze-drying it. In the case of mixing after drying, all may be included.

Specifically, the "extract" may include, but is not limited to, an extract obtained by extracting the herbal medicine of the present invention, a dilution thereof, a concentrate, a dried product, a purified product, or a mixture thereof. The extraction method is not particularly limited, and a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, or a method of a combination thereof may be used, but is not limited thereto.

The herbal medicine extract or fraction thereof used in the composition for improving the constipation of companion animals is water, C1 to C4 alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butyl It may be obtained by extraction with one or more solvents selected from the group consisting of ren glycol, hexane, benzene, and petroleum ether. In one embodiment, the herbal extract or a fraction thereof may be extracted with a mixture of water and a C1 to C4 alcohol selected from the group consisting of maekmundong, saengjihwang, ginseng, chicory, crustaceans, hyssop and angelicae. The concentration of the extraction solvent may be very important to allow the active ingredient of the herbal medicine to be eluted, and these concentrations are, for example, 20 to 60 v/v%, 25 to 60 v/v%, 30 to 60 v/ v%, or 40 to 60 v/v% alcohol aqueous solution, and the alcohol may be specifically ethanol.

The term “fraction” may refer to a result obtained by separating a specific component or a specific group of components from an extract or fermented product in the form of a mixture including several different components. A method for obtaining a fraction may be carried out by a method commonly used in the art, for example, fractionation according to a solvent gradient, fractionation according to a specific solvent composition, but is not limited thereto. Solvents that can be used to obtain the fraction include, for example, polar solvents such as water, distilled water, and alcohol; hexane (Hexane), ethyl acetate (Ethyl acetate), chloroform (Chloroform), a non-polar solvent such as dichloromethane (Dichloromethane); or a combination thereof, but is not limited thereto.

In the present invention, “maekmundong (麥門冬, Liriopis Tuber )” refers to the ampulla of Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler root as medicinal. It is known to be sweet and slightly bitter. It is known to be used as a tonic for dry cough, fever and stuffiness, but it is not known what effect it has on companion animals other than the human body.

In the present invention, "Rehmannia (地黃, Rehmanniae Radix )" is the root of Rehmannia glutinosa Liboschitz ex Steudel (Scrophulariaceae). It is used as a medicinal material in oriental medicine. The root of rehuang is collected and washed as saengjihwang (生地黃), dried and used as geonjihwang, and steamed with alcohol is called sukjihwang. Saengjihwang is known to promote blood coagulation and to increase the contractility of the heart muscle against a weakened heart with the effects of yang blood, death fire, live blood, and hemostasis. It is unknown whether it represents

In the present invention, "Sasam (沙參, Adenophorae Radix )" is a stalk Adenophora tripphylla var. It is the root of japonica Hara or Adenophora stricta Miq (Campanulaceae). The ginseng has a unique flavor, has a viscous nature when chewed, and has a slightly sweet taste and a little cold in nature. In oriental medicine, ginseng is used for dry throat, dry cough, phlegm, seawater, asthma, etc., and is used for dry mouth and throat symptoms, but it is not known what kind of effect it has on companion animals other than the human body.

In the present invention, "chicory ( Cichorium intybus )" is one of the leafy vegetables originating in Northern Europe, the root is fleshy and long, and the stem is 50~ in height, hard, branched and hairy. The leaves from the root face downwards and are divided into feathers. Plants are used as fodder or pasture. The flower is used as a stimulant in the central nervous system and as a medicine to enhance cardiac activity. In Europe and the United States, the coarse roots are powdered and used to make a coffee substitute or as an additive to thicken the color and taste of coffee. Also, in Europe, the root is used as a folk medicine for diuresis, tonicity, health, and to purify the blood. In oriental medicine, the whole plant is used as a medicine, and it is known to be effective against hepatitis jaundice and improve digestion, but it is not known what effect it has on companion animals other than the human body.

In the present invention, "Gac (枳殼, Aurantii Fructus )" is a mature fruit of Poncirus trifoliata Rafinesque, a plant of the Rutaceae family, Rutaceae. and is known to remove red odor. It is also known to be used for chest tightness due to nervousness, digestive disorders, dysentery and its aftereffects, dermatitis and itching, pulmonary edema and hemorrhoids, etc., but it is not known what effect it has on companion animals other than the human body.

In the present invention, "hyssop (牛膝, Achyranthis Radix )" refers to the root of Achyranthes japonica Nakai ( Achyranthes bidentata Blume), which is a herbaceous perennial plant belonging to the Amaranthaceae family. It contains saponin and a large amount of calcium as active ingredients, and showed analgesic action in animal experiments. In addition, it enhances the contraction of the uterus and has a weak diuretic effect, and also exhibits a temporary lowering blood pressure by dilating blood vessels. It is known to have the effect of inducing a woman's menstruation to normal, facilitating diuresis and defecation, lowering blood pressure when there are symptoms such as joint disease, high blood pressure, headache, and dizziness, and relaxing cerebral blood vessel spasms. However, it is not known what kind of effect it has on companion animals other than the human body.

In the present invention, "Angi (當歸, Angelicae Gigantis Radix )" refers to the root of Angelica gigas Nakai or Angelica acutiloba Kitagawa of the umbel family. Pharmacologically, Angelica is known to promote blood flow in the coronary arteries and stimulate the production of red blood cells. However, it is not known what effect it has on companion animals other than the human body.

The above-mentioned herbal medicines have been used as herbal medicines from the past, but animal feed and snacks using them have not been developed yet. In the present invention, a mixture of maekmundong, saengjihwang, ginseng and chicory has an excellent effect on improving cat constipation, and furthermore, when they are mixed with crustaceans, hyssop, angelicae and pollock powder, they show excellent palatability, and significantly excellent efficacy due to synergistic effect It has been established that there is

Specifically, the composition for improving constipation in companion animals exhibits an effect of improving constipation in companion animals, and in particular, the effect of improving constipation in cats is excellent. More specifically, the composition for improving constipation in companion animals of the present invention may have effects of pain between defecation, which are detailed symptoms of cat constipation, reduction of defecation time, and improvement of regularity of defecation.

In addition, the composition for improving constipation in companion animals exhibits an effect of reducing stress, which is considered to be one of the causes of constipation.

Specifically, the composition may include 40 to 50 parts by weight of maekmundong, 40 to 50 parts by weight of saengjihwang, 50 to 60 parts by weight of ginseng, 250 to 400 parts by weight of chicory, and 300 to 480 parts by weight of water, specifically, maekmundong 45 Based on parts by weight, it may include 45 parts by weight of saengjihwang, 55 parts by weight of ginseng, 300 parts by weight of chicory, and 455 parts by weight of water, but is not limited thereto.

In addition, the composition may further include crust, hyssul and Angelica keiskei to increase the effect of improving constipation. Specifically, the composition may include 30 to 40 parts by weight of maekmundong 45 parts by weight, 30 to 40 parts by weight of crust, 20 to 40 parts by weight of mussel, and 20 to 40 parts by weight of angelfish, and specifically, 35 parts by weight of crust relative to 45 parts by weight of maekmundong, It may include 30 parts by weight of wooseul and 30 parts by weight of Angelica.

In addition, the composition of the present invention may include fish such as pollock, salmon, anchovies; Meat, such as beef, chicken breast, pork, duck meat; may further include, and may be included so as to be 25 to 50% by weight based on the weight of the water entering the total composition. In an embodiment of the present invention, pollock powder was used, but the present invention is not limited thereto.

In an embodiment of the present invention, relative to 45 parts by weight of maekmundong, 45 parts by weight of raw ginseng, 55 parts by weight of ginseng, 35 parts by weight of crust, 30 parts by weight of mussel, 30 parts by weight of angelica, 300 parts by weight of chicory, 480 parts by weight of water and 230 parts by weight of pollock powder As a result of preparing a feed containing parts by weight and ingesting it to the cat, the effect of remarkably improving constipation was confirmed (Table 2).

In addition, in one embodiment of the present invention, it was confirmed that the cat's stress was relieved by preparing a feed containing the composition and ingesting it to the cat to significantly reduce various behaviors seen when stressed (Table 3).

In this way, the feed prepared using the extract powder of natural medicines can be used not only as a prescription food for improving constipation, but also as an auxiliary feed mixed with existing feed, and furthermore, by minimizing the smell of herbal medicines of the oriental medicines, palatability is improved. When it is satisfied, it can be used as a snack or nutritional supplement.

One aspect of the present invention provides a food composition comprising the composition. The food means a natural product or processed product containing one or more nutrients, and preferably means a state that pets can eat directly through a certain amount of processing process, , beverages, food additives, dietary supplements and beverage additives are all intended to be included.

In the present invention, dietary supplement refers to a food group or food composition that has added value to act and express the function of the food for a specific purpose using physical, biochemical, and bioengineering methods, etc. It refers to food that is designed and processed to sufficiently express body control functions related to recovery, etc. The health supplement may include a food supplementary additive that is pharmaceutically acceptable, and may further include an appropriate carrier, excipient and diluent commonly used in the manufacture of health supplements.

In addition to the above, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. These components may be used independently or in combination.

In addition, the food composition in the present invention can be used as a food additive that can be added to the food of companion animals.

Another aspect of the present invention provides a pharmaceutical composition for the treatment or prevention of constipation comprising the composition. Specifically, the pharmaceutical composition does not completely exclude the possibility of application to the human body, but may mean that it is applicable to companion animals, particularly cats.

The pharmaceutical composition may further include carriers, excipients and diluents used in the preparation of conventional pharmaceutical compositions.

The carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate or mineral oil.

In addition, the composition can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations, suppositories, and sterile injection solutions. The pharmaceutical composition of the present invention is preferably an oral dosage form, but is not limited thereto.

The solid preparation for oral administration includes tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the composition and its fractions, for example, starch, calcium carbonate, sucrose, lactose, Alternatively, it can be prepared by mixing gelatin or the like. In addition to the above excipients, lubricants such as magnesium stearate and talc may be used.

As the liquid formulation for oral administration, a suspension, an internal solution, an emulsion, a syrup, etc. may be used, and in addition to the simple diluent water and liquid paraffin, various excipients, for example, a wetting agent, a sweetener, a flavoring agent, a preservative, etc. may be included. have.

The pharmaceutical composition comprising the composition for preventing or improving constipation may be administered to a subject in a pharmaceutically effective amount. The “pharmaceutically effective amount” means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is dependent on the patient's type, severity, drug activity, and drug Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including concomitant drugs, and other factors well known in the medical field.

The pharmaceutical composition may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. Taking all of the above factors into consideration, it is preferable to administer an amount capable of obtaining the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

The preferred dosage of the therapeutic composition of the present invention varies depending on the condition and body weight, the severity of the disease, the drug form, the route and period of administration, but may be appropriately selected by those of ordinary skill in the art to which the present invention pertains. have.

Preferably, the composition of the present invention is administered in an amount of 0.1 mg to 10 mg per 1 kg per day. Administration may be administered once a day, or may be administered in several divided doses. The dosage and frequency of administration are not intended to limit the scope of the present invention in any way.

The composition for preventing and improving constipation in companion animals of the present invention significantly improves constipation in companion animals, and at the same time minimizes the objection caused by the smell of herbal medicines to increase palatability, reduces stress in companion animals, and solves the root cause of constipation. characterized.

The effect of the present invention is not limited to the above effect, but it should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 shows the appearance made by forming the composition for preventing and improving constipation in companion animals according to an embodiment of the present invention to a diameter of 1 cm.
Figure 2 shows the appearance confirmed by extracting the digestive tract of the experimental animal after administration of the composition of the present invention (A: appearance of the digestive tract removed, B: the state of measuring the length of the digestive tract)
3 shows the results of confirming the rate of movement of the digestive tract for each experimental group.
4 shows the state of collecting feces on days 1, 3, 5, and 7 for each experimental group.
5 shows the moisture content of feces for each experimental group.
6 shows the relative value of the amount of moisture in the feces on the first day for each experimental group.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it is apparent that the present invention is not limited by the following examples.

Example 1. Composition comprising maekmundong, saengjihwang, ginseng, chicory

A composition for improving cat constipation was prepared, including maekmundong, saengjihwang, ginseng and chicory.

After coarsely pulverizing by mixing with water, finely pulverized using a mixer, freeze-dried and powdered.

Specifically, compared to 45 parts by weight of maekmundong, 45 parts by weight of saengjihwang, 55 parts by weight of ginseng and 55 parts by weight of water were mixed and coarsely pulverized first, and then finely pulverized using a mixer. After the pulverization was completed, the powder was freeze-dried, and the composition of Example 1 was prepared by mixing 300 parts by weight of freeze-dried chicory powder and 455 parts by weight of water and kneading it to a size of about 1 cm in diameter (FIG. 1).

Example 2. Composition further comprising earth hyssop and Angelica quai powder

The composition of Example 1 was prepared in the same manner as in Example 1, but the composition of Example 2 was prepared by further including crust, hyssop, and Angelica basil powder.

Specifically, the crust, wooseul, and angelfish powder included 35 parts by weight of crust, 30 parts by weight of wooseul, and 30 parts by weight of angelicae compared to 45 parts by weight of maekmundong, and water was included so as to be 480 parts by weight. Detailed information related thereto has been described above, and thus has been omitted to avoid excessive repetition of the specification.

Examples 3-4. A composition that increases the preference of cats

Cats have a picky taste and may be stressed in the process of forcibly feeding a composition with low preference, and since sufficient effects do not appear, a composition was prepared by adding pollock powder with high cat preference to the compositions of Examples 1 and 2 . At this time, pollock powder was set as Examples 3 and 4, respectively, so as to be 50% by weight of the water entered in the manufacturing process of Examples 1 and 2.

Comparative Examples 1 to 4. Preparation of compositions with different ratios

Comparative Examples 1 to 4 were prepared by changing only the composition ratio of Examples and the composition. Detailed composition ratios of Examples and Comparative Examples are shown in Table 1 below.

Examples and Comparative Examples

Ingredients (parts by weight) Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Maekmun-dong 45 45 45 45 30 60 45 45 Saengjihwang 45 45 45 45 60 60 45 45 three or three 55 55 55 55 55 60 55 55 tardy - 35 - 35 - - 35 35 hyssop - 30 - 30 - - 30 30 donkey - 30 - 30 - - 30 30 chicory 300 300 300 300 300 265 300 300 Pollock - - 230 230 - - 300 115 water 455 480 455 480 455 455 480 480

Experimental Example 1. Confirmation of the improvement effect of cat constipation

In order to confirm what effect the composition of the present invention has on the improvement of cat constipation, it was confirmed how much the symptoms were relieved when the cat was constipated.

Specifically, 40 constipated cats recruited from veterinary hospitals and cafes were selected, and their sex, weight, and age were considered and divided into 8 groups of 5 each. Then, the compositions of Examples 1 to 4 and Comparative Examples 1 to 4 were fed to each group twice a day for 2 weeks, and the results before and after feeding were confirmed.

To check the effect of improving constipation, how often you go to the toilet (frequency), pain (pain) meaning pain or crying while having a bowel movement, how long you have a bowel movement at a time (time), and regularity of defecation ( regularity) was confirmed how much improved.

At this time, the results were evaluated according to the 5-point scoring method. Specifically, compared to before feeding, if there is no significant difference after 2 weeks of feeding, 0 points are given, and if there is a significant improvement in constipation and the symptom completely disappears, 5 points are given. It is shown in Table 2 below.

Symptom Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 frequency 3.0 3.2 4.6 4.8 2.5 2.8 3.8 3.4 pain 3.2 3.2 4.8 4.9 2.4 2.5 3.5 2.7 hour 3.8 3.3 4.4 4.7 2.5 2.7 3.2 2.8 regularity 3.0 4.4 4.7 4.9 2.6 2.9 3.0 3.0 overall score 3.4 3.5 4.6 4.9 2.5 2.6 3.4 3.0

As shown in the experimental results, it was confirmed that the most important index for improving cat constipation relates to how much the cat is fed the constipation improvement diet without stress. Specifically, in the case of Examples 1 and 2 and Comparative Examples 1 and 2, which had a strong herbal odor due to the absence of pollack powder, overall improvement effect was not observed. It is thought that cats are particularly sensitive animals, and excessive stress occurred in the process of forcibly changing their diet, resulting in a decrease in the overall effect of improving constipation. It was confirmed that a significant effect of improving constipation was confirmed, and in particular, it was confirmed that all of the overall effects were significantly increased when additionally the crust, hyssul, and Angelicae powder were further included.

In Comparative Example 3, in which the proportion of pollock was added excessively, the preference was excellent and the feeding was active, while many feedbacks were received that the effect of improving constipation was lower than expected. Not only did it not go away completely, there was a problem in the degree of preference, but rather, it showed an unexpected result in that the effect was lower than that of Examples 1 and 2 in which pollock was not added.

Experimental Example 2. Confirmation of cat stress relief effect

As described above, in most cases, cat constipation is caused by a combination of various reasons, and stress is one of them. do.

The composition of the present invention not only has an effect of reducing stress by itself by using natural herbal medicines, but also naturally reduces stress as the cat's constipation is improved. was confirmed as

The cat participating in this experiment was the same as in Experimental Example 1 above, and as an indicator for the cat's stressful situation, tense behavior such as excessively looking around, lowering the waist and waving the tail quickly, the behavior of raising the hair upright and mandibles, Long-sounding crying, extreme tension with shaking or accidentally urinating, and open respiration with mouth breathing instead of nose were observed.

However, in this case, since each cat individual has a variety of behavioral ways to express stress, the behavior with the highest frequency among the behaviors described above was intensively checked and evaluated by the guardian rather than uniformly judging it.

Table 3 shows that 0 points were given if the stress-related behavior did not improve at all at the end of two weeks of payment compared to before the payment, and 5 points were given if the behavior completely disappeared.

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 stressful behavior 2.5 3.1 4.4 4.9 2.5 2.2 3.8 3.8

As shown in Table 3, it was confirmed that the stress relief effect showed a tendency that was largely similar to the effect of improving constipation overall, but in particular, Example 4 exhibited a significantly superior effect compared to other Examples and Comparative Examples. It is considered that not only the preference of the feed itself is high, but also the effects of herbal medicines such as crustaceans and hyssop Angelica are expressed.

Experimental Example 3. Improvement of digestive tract mobility and change in fecal moisture content

In order to confirm whether the composition for improving constipation of the present invention actually exhibits an effect of improving constipation in vivo , an experiment using rats was conducted in accordance with the guidelines for improving constipation of the Ministry of Food and Drug Safety.

Specifically, 30 male Sprague-Dawley rats of 4 weeks of age (weight 160-200 g) were obtained and distributed into 6 groups of 5 mice per group.

The breeding environment was a temperature of 20-22℃ and a humidity of 50-55%, and the light-dark cycle was regulated in units of 12 hours. Water and feed were provided ad libitum.

The six groups used in this experiment were a positive control group that did not induce constipation (G1), a negative control group administered only with loperamide (G2), and the composition of Example 1 was administered at a low dose (half of G4). One low-dose group (G3), a group administered with the composition of Example 1 (G4), a group administered only chicory (G5), and a group administered with a composition except chicory from the composition of Example 1 (G6), The results were confirmed on days 1, 3, 5, and 7 during oral administration for one week.

3-1. Confirmation of improvement in digestive tract mobility

For 4-5 experimental animals in each of the 6 groups, after oral administration for 7 days was completed, the effect of improving the rate of digestive tract movement was confirmed by the following experimental method.

First, the experimental animals were prepared by fasting for 16 hours, and after 30 minutes of administration of loperamide at a level of 5 mg/kg, the test substance was orally administered and 10% barium sulfate at a level of 10 mg/kg after 1 hour. administered. Experimental animals were sacrificed 1 hour after administration of 10% barium sulfate.

The abdomen of the experimental animal was incised along the midline, and the intestine was excised from the pylorus of the stomach to the rectum so that the intestine was not cut, and the total length of the digestive tract and the moving distance from the pylorus to the tip of barium sulfate were measured (FIG. 2).

The digestive tract movement rate was calculated using the following formula.

(T=(B/A)×100(%))

*T = the rate of movement of the digestive tract, A = the total length of the digestive tract, B = the movement distance to the tip of the barium sulfate

As a result, as shown in Table 4 and Figure 3, excellent effects were confirmed in both G3 and G4 administered with Example 1 of the present invention, and in the case of G6 except chicory, the effect was significantly lowered.

group individual digestive tract length start End Average range movement rate G1 One 87 61 74 67.5 13 78% 2 88 40 49 44.5 9 51% 3 80 40 46 43 6 54% 4 80 44 52 48 8 60% G2 One 87 3 16 9.5 13 11% 2 96 2 10 6 8 6% 3 100 3 16 9.5 13 10% 4 82 12 23 17.5 11 21% G3 One 71 19 25 22 6 31% 2 76 25 33 29 8 38% 3 60 16 27 21.5 11 36% 4 80 27 36 31.5 9 39% G4 One 59 10 22 16 12 27% 2 92 12 22 17 10 18% 3 76 14 23 18.5 9 24% 4 90 13 38 25.5 25 28% 5 73 22 29 25.5 7 35% G5 One 85 31 37 34 6 40% 2 96 20 32 26 12 27% 3 66 2 21 11.5 19 17% 4 95 42 53 47.5 11 50% G6 One 83 3 8 5.5 5 7% 2 77 6 21 13.5 15 18% 3 80 3 20 11.5 17 14% 4 95 2 26 14 24 15%

3-2. Check for changes in fecal moisture content

When the composition of the present invention is ingested, it is possible to improve constipation by improving the amount of water in the feces. To confirm this, for each of the six groups, feces on the 1st, 3rd, 5th, and 7th days after administration of the test substance were collected to measure the total wet weight (FIG. 4), and the feces were freeze-dried to measure the dry weight. The value obtained by subtracting the dry weight from the wet weight was used as the moisture content of the stool, and is shown in Table 5 and FIG. 5 below.

group Day 1 Day 3 Day 5 Day 7 G1 34% 32% 37% 38% G2 13% 15% 15% 10% G3 10% 19% 25% 30% G4 4% 21% 28% 38% G5 15% 25% 36% 41% G6 6% 24% 27% 21%

However, since there is bound to be a difference in the amount of moisture in the feces for each individual, in order to derive meaningful results from this, the results standardized as relative values based on the value of the first day of feces of each individual are shown in Tables 6 and 6 below. indicated.

group Day 1 Day 3 Day 5 Day 7 G1 1.00 1.02 1.06 1.02 G2 1.00 0.94 1.01 0.97 G3 1.00 1.14 1.21 1.27 G4 1.00 1.23 1.33 1.51 G5 1.00 1.10 1.28 1.39 G6 1.00 1.12 1.17 1.21

As a result, both G3 and G4 administered with the composition of Example 1 of the present invention exhibited excellent effects, and it was confirmed that, in particular, G4 exhibited the most excellent effect.

The description of the present invention described above is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (8)

45 parts by weight of maekmundong, 40 to 50 parts by weight of saengjihwang, 50 to 60 parts by weight of ginseng, 250 to 400 parts by weight of chicory, 30 to 40 parts by weight of crust, 20 to 40 parts by weight of hyssop, 20 to 40 parts by weight of angelfish and 300 to 300 parts by weight of water. prepared including 480 parts by weight,
A composition for preventing or improving constipation in companion animals. delete The method of claim 1,
The composition for improving constipation in companion animals is a raw material for increasing preference and fortifying protein, and further comprising fish or meat, a composition for preventing or improving constipation in companion animals. delete delete A health supplement composition for preventing or improving constipation in companion animals, comprising the composition of claim 3 . A pharmaceutical composition for preventing or treating constipation in companion animals, comprising the composition of claim 3 . Administering the composition of claim 3 in an amount of 0.1 mg to 10 mg per 1 kg per day,
How to prevent or treat constipation in pets.

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