KR102070796B1 - Composition for preventing, alleviating or treating ovarian dysfunction comprising extract of medicinal herb mixture as effective component - Google Patents

Abstract

The present invention relates to a composition for the prevention, improvement or treatment of ovarian dysfunction, which comprises a mixed extract of ephedra, broiler, health, baekbaek, licorice and almond as an active ingredient, ephedra, broiler, health, baekbaek, licorice of the present invention And extracts of passersby has a regulating activity of androgen DHEA production, decreases the content of luteinizing hormone in the blood, decreases the ratio of luteinizing hormone and follicle stimulating hormone (LH / FSH), increases the blood estrogen (Estrogen) and ovarian Since there is an effect of reducing the number of follicular cysts, it can be usefully used for the prevention, improvement or treatment of polycystic ovary syndrome caused by ovarian dysfunction. Therefore, prevention of at least one symptom selected from ovarian dysfunction according to the present invention, such as gonaddrogenemia, early ovarian failure, polycystic ovary syndrome, amenorrhea, hypermenorrhea, dysfunctional uterine bleeding, early menopause, infertility and endometriosis, It can be effectively used for amelioration or treatment.

Description

Composition for preventing, alleviating or treating ovarian dysfunction comprising extract of medicinal herb mixture as effective component

The present invention relates to a composition for the prevention, improvement or treatment of ovarian dysfunction comprising the herbal extract as an active ingredient.

Ovary is a female reproductive organ that produces eggs and secretes sex hormones such as estrogen, progesterone, and testosterone, which not only maintain the health of the female production system but also balance the hormone production system to maintain the quality of life of women. It is an institution that plays an important role in making this happen. Ovarian function can be confirmed by measuring changes in follicle stimulating hormone (FSH), estradiol or AMH (anti-Mullerian hormone), and ovarian dysfunction, hormonal imbalance, premature ovarian failure, polycystic It can cause ovarian syndrome, infertility or early menopause.

Premature ovarian dysfunction among ovarian dysfunctions is similar to menopause before 40 years of age, and ovarian function is stopped. Rarely, it can occur if the number of follicles is sufficient but not growing. The latter can be caused by poor secretion of follicle stimulating hormone (FSH) or failure of the follicle to respond to FSH. The cause of premature ovarian failure is not clear, but chromosomal aberrations, genetic mutations, autoimmune diseases, chemotherapy, radiation therapy, toxic substances, ovarian surgery, ovarian destruction caused by infection or smoking are suggested as causes. Most often unclear.

Polycystic ovary syndrome, on the other hand, is an endocrine metabolism that affects 4-12% of women of childbearing potential. The diagnostic criteria for polycystic ovary syndrome, presented by the National Institutes of Health in 1990, require both chronic ovulation and clinical or biochemical devogenic hyperglycemia. On the other hand, the diagnostic criteria of the European / American Reproductive Endocrinology Association, revised in 2003, include three types of chronic ovulation, clinical or biochemical hyperandrogenemia, and ten small follicles in the form of a bead-shaped beads along the edges of the enlarged ovary. When two or more of the criteria are satisfied, it is defined as polycystic ovary syndrome.

The cause of polycystic ovary syndrome is not yet clear. As with other complications, the development of polycystic ovary syndrome is believed to have both genetic and environmental factors. As the technique developed, the focus of primary pathophysiology shifted from the ovary to the hypothalamus-pituitary axis, and then to insulin deficiency. Although studies on the association between various genetic factors and polycystic ovary syndrome have been reported, there is no clinically applicable genetic test.

Symptoms of polycystic ovary syndrome include (1) oligomenorrhea or amenorrhea, (2) hyperandrogenism and / or hyperandrogenemia, and (3) morphological polycystic ovary morphology. )to be. In addition, based on age and weight surveys, approximately 50% of women with polycystic ovary syndrome compared to controls had luteinizing hormone hypersecretion, insulin resistance, and compensatory hyperinsulin in serum. Biochemical features such as compensatory hyperinsulinemia are common.

As an example of polycystic ovarian syndrome-related diseases, premature ovarian failure occurs when ovarian function does not function normally and is classified as ovulatory cycle insufficiency, luteal insufficiency or ovulation cycle insufficiency. do. Ovulation cycles occur physiologically in puberty and menopause, but infertility in mature women. Progesterone insufficiency causes degeneration of the corpus luteum function quickly, causing infertility and miscarriage. Ovulation cycle negativity is thought to be due to an abnormal length of the follicle because it is more than just the length of the cycle. These ovarian insufficiency can basically appear as a menopausal symptom when the age comes to menopause, but abnormally under 40 years of age, such symptoms may cause difficulty in pregnancy. The worsening of symptoms in these conditions leads to premature menopause.

In addition, dysfunction of the gonadotrophy leading to the hypothalamus-pituitary-ovary caused by a decrease in ovarian function is caused, resulting in physical and mental changes such as sex hormones, lipid and cardiovascular metabolism, bone metabolism, and memory. Menopause begins with menopause and postmenopausal women are at risk for many diseases due to hormonal imbalances, calcium deficiency and increased oxidative stress in the body. In other words, the incidence of diseases such as coronary artery disease, osteoporosis, Alzheimer's disease increases rapidly due to estrogen change in menopause, and in particular, estrogen reduction after menopause causes rapid bone loss.

Ephedra , on the other hand, is an herbaceous shrub belonging to the Ephedraceae . sinica Stapf), dried ephedra ( Ephedra intermedia Schrenk et CA Meyer) or Ephedra equisetina Bunge, dried herb. The origin is from northeastern China and Mongolia and imported from Korea. The active ingredient contains alkaloids. The main ingredients are L-ephedrine and di- pseudoephedrine. The weakness is mild, the taste is bitter and spicy. Animal experiments have been shown to have vasoconstriction, blood pressure rise and sweating. Efficacy is acute pneumonia or acute bronchitis, fever is severe and coughing is prone to antitussive, anti-inflammatory, antipyretic effect. In addition, headache and systemic pain due to the cold and muscle tension is used a lot, but the patient is not suitable for sweating a lot. Excellent antitussive effect, it is also used for pertussis and bronchial asthma. In addition, sweating and diuretic action to remove inflammation of the limb arthritis accompanied by edema, it helps to ease pain and flexing. In Chinese medicine, a lot of drugs or a strong physiological reaction in the human body, so do not use in high blood pressure patients. In addition, overdose or prolonged use may cause insomnia due to cerebral cortex excitability. Representative prescriptions include cold sweats, egrets used as antipyretics and mahyanggamseoktang used for relieving.

Broiler ( Cinnamomum cassia Presl) is the stem bark of Camphor and Lauraceae. Broilers may be of different origin and quality in different regions. In particular, Vietnamese produce broilers up to 5 grades. In addition, since broiler chickens have different harvesting ages and processing methods, commodity specifications are relatively different in terms of scale, relation, plate, heart, and chain. Many are stem-shelled or barrel-shaped, 5-50 cm long, 1.5-5 cm in diameter, and 0.1-0.5 cm thick. The outer side is dark reddish brown and the inner side is reddish brown and smooth. It is easy to bend and its face is reddish brown with a thin layer of light brown color and slightly fibrous. It has a peculiar smell, tastes a little sweet and very, later slightly mucus and astringent.

Health ( Zingiber officinale Roscoe) is a dried root of ginger and Zingiberaceae. It is an irregular lump shape that is pressed flatly by the root stem, and its branches are divided into fingers. The length is 2 ~ 4cm and the diameter is 1 ~ 2cm. The outer surface is grayish white to light grayish brown with white powder, and the light grayish yellow skin is intact or peeled off. Each part of the branch is slightly pressed, slightly bent egg-long egg shape, and there are bumps protruding on both sides. The quality is reliable.

Chongpa ( Allium) fistulosum L.) It is a fresh scale of Liliaceae. It is a long columnar shape with several thin roots on one end, and the other side has a cut off section with concentric circles. It is 3 ~ 10cm long and 1 ~ 2cm in diameter. The outer surface is white and there is a clear vertical parallel vein. It has a green onion smell and tastes a bit sweet and spicy. It is grown in various parts of Korea. Known pharmacological components include allicin (allicin) and diallyl-monosulfide (pharmaceutical action is known as antipyretic action, diuretic action, dry stomach action, expectorant action is known. In addition, volatile components, etc. act on the enzyme system of bacteria to inhibit the growth of diphtheria bacteria, tuberculosis bacteria, erythropoies, staphylococci, streptococci, and the water immersion agent (1: 1) inhibits the growth of various skin fungi, There is a function to regulate the tension of blood vessels.

Licorice ( Glycyrrhiza uralensis ) has a peculiar smell, tastes sweet, harmonizes the toxicity of all medicines, and makes the drug appear well, controls the heat and morale of the book, communicates all blood vessels, and strengthens muscles and bones. Pharmacological action is effective in detoxification, hepatitis, urticaria, dermatitis, eczema. Jinhae, expectoration, muscle relaxation, diuretic, anti-inflammatory action and suppresses peptic ulcer. The outer skin of licorice is reddish brown or dark brown with vertical wrinkles, sometimes with stamina, shoots and scaly leaves. Peeled licorice is light yellow on the outside and fibrous.

Pedestrian Apricot Tree ( Prunus) armeniaca Linne var. ansu Maximowicz, Prunus mandshurica Koehne var. glabra Nakai), Siberian Apricot ( Prunus) A mature seed of the Rosaceae plant of the Rosaceae family of sibirica Linne or Prunus armeniaca Linne, which is warm, sweet, bitter and poisonous. Heals coughs, treats his teeth, and breathes his breath, and heals it, causing sweat and eliminating dog poison. In addition, it contains a large amount of unsaturated fatty acid, so it has excellent skin soothing and softening effect.

Prior art related to ovarian dysfunction is disclosed in Korean Registered Patent No. 1794836 for a composition for improving menopausal symptoms containing Angelica, cheonggung and broiler as an active ingredient, Korean Patent Publication No. 2016-0011756 Although a phytoestrogens-containing functional food composition having an effect of preventing and improving menopausal or menopausal symptoms has been disclosed, a composition for preventing, ameliorating or treating ovarian dysfunction comprising the herbal extract of the present invention as an active ingredient has been disclosed. There is no.

The present invention is derived from the above requirements, the present invention relates to a composition for the prevention, improvement or treatment of ovarian dysfunction comprising a mixed extract of ephedra, broiler, health, baekbaek, licorice and passersby as an active ingredient. , The mixed extract of ephedra, broiler, health, ginseng, licorice and almonds of the present invention has a regulating activity of androgen DHEA production, reducing the content of luteinizing hormone in the blood, the ratio of luteinizing hormone and follicle stimulating hormone (LH / FSH) The present invention has been completed by confirming that there is a decrease in blood, an increase in blood estrogen, and a decrease in the number of follicular cysts in the ovary.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention or treatment of ovarian dysfunction, comprising a mixed extract of ephedra, broiler, health, baekbaek, licorice and passers-by as an active ingredient.

In addition, the present invention provides a health functional food composition for the prevention or improvement of ovarian dysfunction, which comprises a mixed extract of ephedra, broiler, health, baekbaek, licorice and almond as an active ingredient.

The present invention relates to a composition for the prevention, improvement or treatment of ovarian dysfunction, which comprises a mixed extract of ephedra, broiler, health, baekbaek, licorice and almond as an active ingredient, ephedra, broiler, health, baekbaek, licorice of the present invention And extracts of passersby has a regulating activity of androgen DHEA production, decreases the content of luteinizing hormone in the blood, decreases the ratio of luteinizing hormone and follicle stimulating hormone (LH / FSH), increase the effect of blood estrogen (Estrogen) and ovary It is effective in reducing the number of follicular cysts.

1 is a result confirming the effect of regulating the androgen DHEA production of the mixed extract of the ephedra, broiler, health, baekbaek, licorice and almond of the present invention. FOR (+) is a group of cells treated with 10 μM of Forskolin to induce excessive secretion of androgens, MET is a positive control cell treated with 1 mM of metformin, and PIO is 10 μM of pioglitazone (pioglitazone). ) Cells of the positive control group. *** indicates that DHEA production was significantly increased in the androgen-induced cell group treated with phospholine compared to normal group (CON), meaning p <0.001, and ### treated with phospholine Compared to one cell group, the amount of DHEA production in the cell group treated with the mixed extract of ephedra, broiler, health, ginseng, licorice and algae of the present invention was statistically significant, which means p <0.001.
Figure 2 is a mixture of the ephedra, broiler, health, baekbaek, licorice and passersby of the present invention administered to a polycystic ovary syndrome (PCOS) animal model to reduce the luteinizing hormone (LH) The effect was confirmed.
Figure 3 is a mixture of the ephedra, broiler, health, baekbaek, licorice and almond of the present invention (medicinal herb mixed extract) is administered to a polycystic ovary syndrome (PCOS) animal model, blood luteinizing hormone (LH) / The effect of reducing the follicle stimulating hormone (FSH) ratio was confirmed.
4 is a mixed extract (herbal medicinal herb extract) of ephedra, broiler, health, baekbaek, licorice and passerby of the present invention to the polycystic ovary syndrome (PCOS) animal model to confirm the effect of increasing the estrogen in the blood .

The present invention relates to a pharmaceutical composition for the prevention or treatment of ovarian dysfunction, which comprises a mixed extract of ephedra, broiler, health, baekbaek, licorice and almond as an active ingredient. The mixed extract of ephedra, broiler, health, baekbaek, licorice and almond is preferably extracted by mixing in a weight ratio of 3: 2: 0.6: 1: 1: 1, but is not limited thereto.

The mixed extract of ephedra, broiler, health, baekbaek, licorice and almond can be prepared by a method comprising the following steps, but is not limited thereto:

(1) extracting by adding an extraction solvent to a mixture of ephedra, broiler, health, ginseng, licorice and almonds;

(2) filtering the extract of step (1); And

(3) concentrating and drying the filtered extract of step (2) to produce an extract.

The extraction solvent in the step (1) is preferably selected from water, C ₁ ~ C ₄ lower alcohol or a mixture thereof, more preferably water, but is not limited thereto.

In the above production method, the extraction method may use all conventional methods known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction. The extraction solvent is preferably extracted by adding 1 to 20 times the total weight of the dried herbal medicine mixture extract, more preferably 5 to 15 times, even more preferably 10 times. Extraction temperature is preferably 60 ~ 100 ℃ but is not limited thereto. In addition, the extraction time is preferably 1 to 48 hours, more preferably 1 to 24 hours, most preferably 3 hours is not limited thereto. In the above method, the concentration of step (3) is preferably, but not limited to, using a vacuum rotary concentrator or a vacuum rotary evaporator. In addition, the drying is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or lyophilization, more preferably freeze-drying, but is not limited thereto.

The ovarian dysfunction is preferably at least one selected from folic and hyperglycemia, early ovarian failure, polycystic ovary syndrome, amenorrhea, hypermenorrhea, dysfunctional uterine bleeding, early menopause, infertility and endometriosis. no.

In addition to the mixed extract of ephedra, broiler, health, baekbaek, licorice and almond may further comprise a pharmaceutically acceptable carrier, excipient or diluent.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and when parenteral administration, it is preferable to select an external skin or intraperitoneal, rectal, intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injection method.

The pharmaceutical compositions of the present invention may be formulated using diluents or excipients, such as fillers, extenders, binders, wetting agents, disintegrants, surfactants and the like. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may comprise at least one excipient such as starch, calcium carbonate, sucrose or lactose (at least one compound). lactose) and gelatin. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As a base of suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycero gelatin and the like can be used.

The composition according to the invention is administered in a pharmaceutically effective amount. In the present invention, “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level refers to the type, severity, and activity of the patient's disease. , Drug sensitivity, time of administration, route of administration and rate of release, duration of treatment, factors including concurrent use of drugs, and other factors well known in the medical arts. The compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be single or multiple doses. Taking all of the above factors into consideration, it is important to administer an amount that can achieve the maximum effect with a minimum amount without side effects, which can be readily determined by one skilled in the art.

Dosage of the composition of the present invention can be used in a variety of ranges depending on the weight, age, sex, health status, diet, time of administration, administration method, excretion rate and severity of the patient.

In addition, the present invention relates to a health functional food composition for the prevention or improvement of ovarian dysfunction, which comprises a mixed extract of ephedra, broiler, health, baekbaek, licorice and almond as an active ingredient.

The ovarian dysfunction is preferably at least one selected from early ovarian failure, polycystic ovary syndrome, hyperandrogenemia, amenorrhea, hypermenorrhea, dysfunctional uterine bleeding, premature menopause, infertility and endometriosis. no.

Health functional food composition for the prevention or improvement of ovarian dysfunction comprising the mixed extract of the ephedra, broiler, health, baekbaek, licorice and almonds as an active ingredient in powders, granules, pills, tablets, capsules, candy, syrups and beverages It may be prepared by any one selected or by adding as an ingredient of a food, and may be appropriately prepared according to a conventional method.

Examples of foods to which the mixed extract of ephedra, broiler, health, baekbaek, licorice and almond of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, Dairy products, including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes can be any one of the forms selected, including all the health functional foods in the conventional sense.

The dietary supplement includes various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. Others may contain pulp for the production of natural fruit juices and vegetable drinks. These components can be used independently or in combination.

The dietary supplement composition of the present invention may contain various flavors or natural carbohydrates as additional ingredients. The natural carbohydrates are sugars such as glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as tautin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for explaining the present invention more specifically, it is obvious to those skilled in the art that the scope of the present invention is not limited by them.

1.Androgen DHEA ( Dehydroepiandrosterone Control of production

In one embodiment of the present invention, to treat the human adrenal cell line (H295R) 10μM of Forskolin (Forskolin) to increase the production of androgen DHEA (Dehydroepiandrosterone), to determine whether to reduce the amount of androgen DHEA, 50, 100 and 200 μg / ml of ephedra, broiler, health, ginseng, licorice and almonds were treated.

2. Blood luteinizing hormone / follicle stimulating hormone ratio ( LH Of FSH  ratio), blood luteinizing hormone ( luteinizing  hormone; LH ), Blood estrogen and ovaries Follicle changes in the number of follicular cysts

Female SD (Sprague-Dawly) rats used as experimental animals were supplied from Orient Bio, Inc. The experimental animals were used after adapting for 7 days at the joint animal laboratory breeding facility of Chungnam National University.

The experimental method was deliberated by the Animal Experimentation Ethics Committee of Chungnam National University, and the breeding and maintenance of experimental animals was carried out at the temperature of 23 ± 1 ℃, humidity of 50 ± 10% and 12 hours in accordance with the animal care and use regulations of Chungnam National University Animal Laboratory. Two dogs were housed in an individually ventilated cage (IVC) in a nursery that was kept constant in light and dark cycles.

Blood luteinizing hormone / follicle stimulating hormone ratio (LH / FSH ratio), blood luteinizaing hormone, blood estrogen and estrogen of the mixed extract of ephedra, broiler, health, baekbaek, licorice and almond according to the present invention The PCOS animal model for determining the change in the number of follicular cysts in the ovary was 1 mg / kg / BW after inserting a sustained-release formulation pellet with a release of 0.1 mg / kg / day into the rat subcutaneous. Letrozole was established by inducing the symptoms of PCOS through daily oral administration for 2 weeks.

Example  1. ephedra, broiler, health, Total back Preparation of Mixed Extracts of Licorice, Licorice and Almond

The mixed extract of ephedra, broiler, health, baekbaek, licorice and almond of the present invention is a mixture of ephedra, broiler, health, baekbaek, licorice and almond in a weight ratio of dry 3: 2: 0.6: 1: 1: 1 After adding 10 times water and leaching for 3 hours at 100 ° C., a mixed extract of ephedra, broiler, health, baekbaek, licorice and almonds was obtained. The mixed extract of ephedra, broiler, health, baekbaek, licorice and almonds was filtered with a filter of 5㎛ qualitative filter, concentrated in a vacuum rotary concentrator and lyophilized using a freeze dryer.

Example  2. ephedra, broiler, health, Total back Control Effect of Androgen DHEA in Mixed Extracts of Licorice, Licorice and Almond

As a result of treatment of 50, 100 and 200 μg / ml ephedra, broiler, health, ginseng, licorice and algae mixed extracts to human adrenal cell line (H295R), androgen dehydroepiandrosterone (DHEA) production was significant as shown in FIG. It was confirmed that the concentration was suppressed.

Example  3. ephedra, broiler, health, Total back Progesterone Hormone Through Administration of Mixed Extracts of LH ) Check for changes

Animal model was used to determine the change in the content of luteinizing hormone (LH) in blood. In the PCOS-derived 0.1 mg / kg / day letrozole-administered group, the blood luteinizing hormone (LH) content was significantly increased, and 500 mg / kg / day ephedra, broiler, health, total back, The experimental group (PCOS + mixed herbal extracts) mixed with licorice and algae mixed extract confirmed that the blood luteinizing hormone (LH) was statistically significantly reduced (Fig. 2).

Example  4. ephedra, broiler, health, Total back Progesterone / Follicle Stimulating Hormone Ratio in Blood of Mixed Extracts of LH Of FSH  change)

PCOS group administered 0.1mg / kg / day letrozole, and mixed extract of 0.1mg / kg / day letrozole and 500mg / kg / day ephedra, broiler, health, ginseng, licorice and almond The experiment was divided into (PCOS + herbal medicine mixed extract). PCOS was induced by administration of 0.1 mg / kg / day letrozole for two weeks in the first half of the entire experimental period, and natural recovery (PCOS) or bone except for administration of letrozole for the second two weeks. A mixed extract of ephedra, broiler, health, baekbaek, licorice and almond of the invention was administered.

Then, as a result of confirming the change in the blood luteinizing hormone / follicle stimulating hormone ratio (LH / FSH ratio), as shown in Figure 3, in the PCOS group induced by the administration of 0.1mg / kg / day letrozole Blood luteinizing hormone / follicle stimulating hormone ratio (LH / FSH ratio) was significantly increased, compared to the 500 mg / kg / day ephedra, broiler, health, ginseng, licorice and passerby group It was confirmed that the LH / FSH ratio in blood corpus luteinizing hormone / follicle stimulating hormone (FIG. 3) was significantly reduced.

Example  5. ephedra, broiler, health, Total back Estrogen through the administration of mixed extracts of licorice and licorice ( Estrogen ) Check for changes

Animal models were used to determine changes in the content of blood estrogens. In the PCOS-derived 0.1 mg / kg / day letrozole group, the blood estrogen content was statistically significantly reduced, and the 500 mg / kg / day ephedra, broiler, health, ginseng, licorice and algae mixture of the present invention In the experimental group mixed with the extract (PCOS + medicinal herb mixture extract) it was confirmed that the blood estrogen significantly increased (Fig. 4).

Example  6. ephedra, broiler, health, Total back In the ovary through administration of a mixed extract of licorice, licorice and almond Follicle  Effect on Number Changes

As a result of confirming the change in the number of follicles in the ovary of the mixed extract of ephedra, broiler, health, baekbaek, licorice and passersby, the number of cystic follicles in the ovary was significantly increased in the PCOS-induced animal model group, but the present invention In the experimental group (PCOS + medicinal herbs mixed extract) administered the mixed extract of 500mg / kg / day ephedra, broiler, health, ginseng, licorice and almonds was found that the number of follicular follicles in the ovary was significantly reduced (Table 1).

CON PCOS PCOS + Herbal Medicine Blend Extract Follicle count 7.83 ± 2.64 13.50 ± 4.37 ^a 4.88 ± 3.36 ^b

Claims (7)

Ovarian dysfunction of any one selected from ephedra, broiler, health, baekbaek, licorice and passersby as an active ingredient, polycystic ovary syndrome, hyperandrogenemia, amenorrhea, hypermenorrhea and dysfunctional uterine bleeding Pharmaceutical composition for the prophylaxis or treatment of. The polycystic ovary syndrome according to claim 1, wherein the solvent of the mixed extract of ephedra, broiler, health, ginseng, licorice and almond is water, lower alcohol of C ₁ to C ₄ or a mixture thereof. ), A pharmaceutical composition for the prophylaxis or treatment of any one ovarian dysfunction selected from folic acid, amenorrhea, hypermenstrual and dysfunctional uterine bleeding. delete According to claim 1, Polycystic ovary syndrome (polycystic ovary syndrome), sodrogenic, amenorrhea, characterized in that it further comprises a carrier, excipient or diluent in addition to the mixed extract of ephedra, broiler, health, ginseng, licorice and almond Pharmaceutical composition for the prevention or treatment of any one ovarian dysfunction selected from hypermenstrual and dysfunctional uterine bleeding. Ovarian dysfunction of any one selected from ephedra, broiler, health, baekbaek, licorice and passersby as an active ingredient, polycystic ovary syndrome, hyperandrogenemia, amenorrhea, hypermenorrhea and dysfunctional uterine bleeding Health functional food composition for the prevention or improvement of. delete The method of claim 5, wherein the composition is made of powder, granules, pills, tablets, capsules, candy, syrups and beverages in any one of the formulations selected from polycystic ovary syndrome (polycystic ovary syndrome), develogenemia, Health functional food composition for the prevention or improvement of any one ovarian dysfunction selected from amenorrhea, hypermenorrhea and dysfunctional uterine bleeding.

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