KR100496622B1 - Alcohol fermented food or pharmaceutical composition for prevention of obesity and process for preparation thereof - Google Patents

Abstract

The present invention relates to a mixed herbal medicine extract and a pharmaceutical composition or health food for preventing or treating obesity containing the extract as an active ingredient, and more specifically, to the tuber, green tea, tofu, garlic, hawthorn, raw pine needles and jinjin mugwort The mixed herbal medicine extract extracted by adding water or an aqueous alcohol solution or an extract fermented by adding rice, koji and yeast to the extract and the pharmaceutical composition for preventing or treating obesity containing the extract as an active ingredient or health It is about food. The extract of the present invention can be useful for preventing or treating obesity by inhibiting obesity due to a high fat diet and reducing blood cholesterol and triglycerides.

Description

Alcohol fermented food or pharmaceutical composition for the prevention of obesity and a method for preparing the same {Alcohol fermented food or pharmaceutical composition for prevention of obesity and process for preparation

The present invention relates to a mixed herbal medicine extract and a pharmaceutical composition or health food for preventing or treating obesity containing the extract as an active ingredient, and more specifically, to the tuber, green tea, tofu, garlic, hawthorn, raw pine needles and jinjin mugwort The mixed herbal medicine extract extracted by adding water or an aqueous alcohol solution or an extract fermented by adding rice, koji and yeast to the extract and the pharmaceutical composition for preventing or treating obesity containing the extract as an active ingredient or health It is about food.

Obesity is a phenomenon in which the remaining amount of calories consumed is converted to fat and accumulated in various parts of the body, especially the subcutaneous tissue and abdominal cavity. Triglyceride is a compound obtained by esterifying glycerin and three molecules of fatty acid. Triglyceride is a triglyceride synthesized from carbohydrates and stored in animal fat tissue. Triglycerides stored in adipose tissue are broken down into nonesterifide fatty acid (NEFA) and glycerol when the sugar is insufficient as an energy source and released into the blood. Used as an energy source, the remaining non-esterified fatty acids turn back to triglycerides in the liver. When it enters the blood again, it is called endogenous triglycerides. Excessive amounts of triglycerides are closely related to atherosclerosis and coronary artery disease.

Obese patients have a lot of fat accumulated in the body than the amount of fat required for the function of adipose tissue, causing this is a person with impaired normal biochemical and physiological function in the human body. Obesity not only causes various diseases such as diabetes, hyperlipidemia, hypertension and coronary artery disease, and joint diseases, but also makes normal social life impossible.

Various efforts such as the development of a weight loss agent, an appetite suppressant or a fat absorption inhibitor to reduce the adverse effects of obesity are being progressed. In particular, the development of diet products to meet the desire for a slim body as well as the purpose of preventing or treating obesity has attracted much attention. However, products developed for this purpose have many problems. For example, diets using diuretics and laxatives, which are easily accessible at low cost, can reduce moisture in the body and provide a temporary weight loss effect. However, it is not related to fat removal, which is the cause of obesity, and excessive use can lead to abnormal heart rhythms due to dehydration and electrolyte drainage. In addition, this type of drug is likely to become habitual, reducing the body's excretory function may cause side effects such as edema and constipation. In addition, diet products using an appetite suppressant may cause side effects such as blood pressure increase and abdominal pain, nervousness, insomnia, and dizziness due to nervous excitement. Currently used fiber preparations are processed fiber, not to lose weight, but to give a feeling of satiety and can cause nutritional deficiency in long-term overdose.

Recently, a lot of research has been focused on the development of the prevention or treatment of obesity, but there are no products that can effectively suppress obesity without side effects.

Accordingly, the present inventors have tried to develop a composition exhibiting the effect of suppressing obesity without side effects by using foods, such as food safety ensured, as a result, while solving the problems of the prior art, such as side effects of anti-obesity drugs The present invention has been completed by developing a herbal extract showing an excellent effect on the treatment of obesity.

It is an object of the present invention to provide an extract of a mixture comprising a defect, green tea, tofu, garlic, hawthorn, raw pine needles and ginseng wormwood and a pharmaceutical composition or health food for preventing or treating obesity containing the extract as an active ingredient.

In order to achieve the above object, the present invention is to extract the mixture containing the deficiency, green tea, tofu, garlic, hawthorn, raw pine needles and ginseng mugwort by the addition of water or an aqueous alcohol solution or further to the extract, rice, yeast and yeast To give fermented extract.

The present invention also provides a pharmaceutical composition for preventing or treating obesity containing the extract as an active ingredient.

The present invention also provides a health food for preventing or treating obesity containing the extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a mixed herbal extract extracted by adding water or an aqueous alcohol solution to the mixture containing the deficiency, green tea, tofu, garlic, hawthorn, raw pine needles and phosphorus mugwort.

Cassia tora (Cassia obtusifolia L.) in the have a herbal, and green tea (Thea sinensis L.) is cheongyeol detoxification and Cheongshim diuretic in young leaves of tea plant which is used to dress the seed, diuretic, ophthalmic diseases annual herb of Cassia tora . Eucommia ulmoides Oliver is a bark of the cedar tree, which has a constant blood pressure lowering and tonic effect. Garlic ( Allium sativum var. Pekinense ) shows good health, insecticidal, and detoxification. Sansa ( Crataegus Pinnatifida Bunge) is a dried fruit of the hawthorn, which promotes secretion of gastric juice, sustained coercive action, and antibacterial activity. Green pine needles ( Pinus densiflora Siebold et Zuccarini) are effective in preventing back pain, chronic bronchitis and cold. Artemisia capillaris is effective for promoting bile secretion, jaundice and infection.

The mixed herbal medicine of the present invention is a deficiency: Green tea: Tofu: Garlic: Sansa: Green pine needles: Injin mugwort 1-3: 0.5-1: 2-7: 1-3: 0.5-1: 3-7: 3-7 It is preferable to include (w / w), and the mixture is prepared by extracting with water or an aqueous alcohol solution.

The weight ratios of the above-mentioned herbal medicines are obtained on the basis of repeated experimental results, and when lower than the lower limit, the pharmacological effect of the component may be reduced, and when higher than the upper limit, the pharmacological effect of the other components may be reduced. There is a fear that cooperation will be reduced.

The present invention provides hot water extract and dried under reduced pressure by adding water to the mixture.

The present invention also provides an extract, which is extracted by adding an aqueous alcohol solution to the mixture and dried under reduced pressure. The aqueous alcohol solution may be selected from the group consisting of 5 to 100% ethyl alcohol (ethyl alcohol) and 5 to 100% methyl alcohol (methyl alcohol).

In addition, the present invention provides an extract fermented by adding rice, yeast and yeast to the extract of the mixed herbal medicines.

In the rice: yeast: yeast is preferably added by mixing 20-80: 10-40: 0-2 weight ratio (w / w).

In the above fermentation is preferably fermented at 15 to 30 ℃ for 5 to 15 days, the fermentation broth is filtered and separated into a liquid and a solid portion, the liquid portion is heated to 55 to 60 ℃ to prepare a main .

In order to find out the effect of preventing or treating obesity of the extract prepared as described above, rats were divided into three groups: normal diet group, high fat diet group (hereinafter referred to as control group) and high fat and composition diet group of the present invention (hereinafter referred to as experimental group). As a result of measuring the weight change, the average weight of the control group was increased compared to the normal diet group and the experimental group was decreased compared to the control group (see FIG. 1 ). The above results indicate that the high fat diet-induced weight gain can be effectively controlled because the extract of the present invention exhibits an excellent weight increase inhibitory effect.

In addition, the total cholesterol, triglycerides, low-density lipoprotein (LDL), high-density lipoproteins (HDL), which are lipid components of serum, were measured. Although the experimental group showed a low increase rate compared to the control group (see FIG. 2 ), the HDL and LDL contents were not significantly changed (see FIG. 3 ). However, the triglyceride was increased in the control group compared to the normal diet group and the experimental group was decreased compared to the control group (see FIG. 4 ). The results indicate that the extract of the present invention shows an excellent neutral fat inhibitory effect, so that the high fat diet-inducing serum lipid content level can be effectively controlled.

Therefore, the extract of the present invention can be useful for the prevention or treatment of obesity by reducing the concentration of triglycerides in the blood and inhibiting the accumulation of fat in the body.

The present invention also provides a pharmaceutical composition for preventing or treating obesity containing the extract as an active ingredient.

The extract of the present invention is preferably in the form of a liquid, but if necessary, it may be prepared in the form of pills, granules, tablets or capsules, and may be used in other dosage forms when necessary. In addition, when the extract of the present invention is prepared with a suitable concentration of the subject, it is most preferable to prepare it as a liquid formulation.

The extract of the present invention can be administered orally or parenterally during clinical administration and can be used in the form of general pharmaceutical preparations.

That is, the extract of the present invention may be administered in various oral and parenteral dosage forms during actual clinical administration, and when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. that are commonly used. Is prepared using. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid form extracts may contain at least one excipient such as starch, calcium carbonate, sucrose, or the like. Or it is prepared by mixing lactose (Lactose), gelatin and the like. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The effective dose of the extract of the present invention is 0.5 to 3.0 ml / kg, preferably 1 to 2 ml / kg, 1 to 1 day, based on the main ingredient produced by the fermentation prepared by the method described below. Three times may be administered.

The extract of the present invention is preferably in a mixture of 50 to 150 g of glycine, 25 to 50 g of green tea, 100 to 350 g of tofu, 50 to 150 g of garlic, 25 to 50 g of hawthorn, 150 to 350 g of pine needles (raw), and 150 to 350 g of ginseng root. Pour 5,000 to 8,000 ml of water, decoction for about 2 to 4 hours, concentrate to about 3,000 ml to obtain an extract, and mix uniformly the taste of rice 1 to 4 kg, 0.2 to 1 kg of yeast and 0 to 0.1 kg of yeast. The extract was poured into the solution, fermented at 20 to 27 ° C. for 5 to 10 days, filtered to prepare a subject, and then purified water was adjusted to 3,000 ml. A typical single dose of the composition is 1.0-2.0 ml per kg of body weight and is taken three times a day. For example, in the case of an adult weighing 60 kg, 60-120 ml is taken three times a day. When taken at such a dose, the preparation is about 8-15 days. However, the composition of the present invention can increase or decrease the dosage depending on the weight, age, sex, severity of the patient and digestion. In other formulations, an appropriate amount calculated in consideration of the liquid dosage can be administered orally or parenterally. As early as 2 to 3 days after taking the symptoms of the symptoms can be reduced, but there are individual differences.

The present invention also provides a health food for preventing or treating obesity containing the extract as an active ingredient.

When the extract of the present invention is used as a food, the extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, the extract of the present invention may be added in an amount of 0.001 to 50% by weight, preferably 0.01 to 5% by weight based on the raw materials in the manufacture of food or beverage. An effective dose of the extract of the present invention may be used in accordance with the effective dose of the pharmaceutical composition, but may be less than the above range in the case of long-term intake for the purpose of health and hygiene or health control, Since there is no problem in terms of safety, it is certain that it can be used in an amount above the above range.

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1 Preparation of Mixed Herbal Medicine Extract 1

5 g of water, 75 g of green tea, 25 g of green tea, 150 g of garlic, 75 g of garlic, 30 g of hawthorn, 150 g of pine needles (raw), and 150 g of ginseng mugwort were poured into the mixture.

Example 2 Preparation of Mixed Herbal Medicine Extract 2

A mixture of 75 g of Clarifier, 25 g of green tea, 150 g of Tofu, 75 g of garlic, 30 g of hawthorn, 150 g of pine needles (raw), and 150 g of Injin mugwort were placed in 1 L of an aqueous 70% methanol solution and stirred at room temperature for 2 days. After 2 days, the solution was filtered with filter paper and the process was repeated three times. The filtrate was concentrated under reduced pressure to remove methanol, and then freeze-dried to remove moisture to obtain a mixed herbal extract.

Example 3 Preparation of Mixed Herbal Medicine Extract 3

5 g of water was added to the mixture of 75 g of the gallows, 25 g of green tea, 150 g of Tofu, 75 g of garlic, 30 g of hawthorn, 150 g of pine needles (raw), and 150 g of Injin mugwort. After the mixture of steamed rice made from 2 kg of rice, 1 kg of yeast and 50 g of yeast was uniformly mixed, the extract was poured and fermented at about 20 to 25 ° C. for about 7 days. The fermentation broth is filtered to separate the liquid and solid portions, and the liquid portion is heated to 55-60 ° C. to inactivate the enzymes.

Preparation Example 1 Preparation of Injection Solution

Injection solution containing 10 mg of the active ingredient was prepared by the following method.

100 g of 1 g of the extract prepared above, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water. The solution was bottled and sterilized by heating at 20 ° C. for 30 minutes.

The components of the injection solution are as follows.

Mixed Herbal Extract 1 g

0.6 g sodium chloride

0.1 g of ascorbic acid

Distilled Water Determination

Preparation Example 2 Preparation of Syrup

Syrup containing the extract of the present invention as an active ingredient 2% (weight / volume) is prepared by the following method.

The mixed herbal extract, saccharin and sugar were dissolved in 80 ml of warm water. After the solution was cooled, a solution consisting of glycerin, spices, ethanol, sorbic acid and distilled water was prepared and mixed therein. Water was added to this mixture to 100 ml.

The components of the syrup are as follows.

2 g of mixed herbal medicine extract

Saccharin 0.8 g

25.4 g per

Glycerin 8.0 g

0.04 g of spices

Ethanol 4.0 g

0.4 g of sorbic acid

Distilled Water Determination

Preparation Example 3 Preparation of Beverage

The present inventors prepared a beverage composition containing the mixed herbal medicine extract as an active ingredient as follows.

522 mg of honey

Chioctosanamide 5 mg

Nicotinamide 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Mixed Herbal Medicine Extract 0.48 ~ 1.28 ㎎

200 ml of water

With the above composition and content, drinks were prepared using conventional methods.

Experimental Example 1 Weight Loss Effect

Experimental animals consisted of 8 male rats of Sprague-Dawley species per group, and were divided into three groups of the normal diet group, the high fat diet group (hereinafter referred to as the control group), and the high fat and present invention composition diet group (hereinafter referred to as the experimental group). Classified as dogs. The normal group provided normal diet and the control group made up 40% of total calories as fat calorie and added 0.05% cholesterol. The experimental group made the feed by adding the extract of the present invention converted to a high fat diet at the same rate as the adult daily intake dose. That is, the feed prepared in Example 3 per kilogram of body weight was prepared to consume about 5 ml per day (300 ml per day for 60 kg body weight, 1 ml per day for 200 g rats). Since the feed dose of rats (about 200 g) per day is about 15 g, the feed was prepared to contain 1 ml of the extract of the present invention in 15 g of the feed. Water and feed were supplied without restriction. Weight change was measured about once every 10 days for 33 days.

As a result, the average body weight of the control group increased about 37.3% after 33 days compared to the normal diet group, and the experimental group decreased about 15.3% compared to the control group. When only the weight gain was compared (when the weight gain of the control group was calculated as 100%), the experimental group confirmed an effect of about 59.3% reduction compared to the control group ( FIG. 1 , Table 1 ). The results indicate that the high fat diet-induced weight gain can be effectively controlled because the extract of the present invention exhibits an excellent weight increase inhibitory effect.

Experimental group Initial weight (g) Weight after 33 days (g) Weight gain (g) Normal diet. 171.25 ± 1.43 268.0 ± 2.04 96.75 ± 2.28 High fat diet group (control group) 170.0 ± 2.12 324.5 ± 5.05 154.5 ± 6.22 High fat diet + invention extract group (experimental group) 175.25 ± 2.28 306.25 ± 22.66 131.0 ± 22.09

Experimental Example 2 Blood Lipid Content Analysis

After the 33-day experimental diet was fasted for 12 hours, anesthetized with ethyl ether, and then opened, blood was collected by a disposable syringe in the ventricles, and serum obtained by centrifugation at 3,000 rpm for 20 minutes was used as a sample. Serum lipid, total cholesterol, triglyceride, low-density lipoprotein (LDL) and high-density lipoproteins (HDL) were measured.

As a result, the total cholesterol of the control group increased about 6.5% compared to the normal diet group, but the experimental group showed a low increase rate compared to the control group ( FIG. 2 ). In contrast, the HDL and LDL contents did not change significantly ( FIG. 3 ). However, the triglyceride control group increased 8.8% compared to the normal diet group and the experimental group 16.8% decrease compared to the control group ( Fig. 4 ). The results indicate that the extract of the present invention exhibits excellent triglyceride inhibitory effect in serum, so that the high fat diet-inducing blood lipid content level can be effectively controlled. In addition, blood glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin were measured, but there were no significant changes.

Experimental Example 3 Oral Acute Toxicity in Rats

Acute toxicity test was performed using 6-week-old SPF SD rats. Two animals per group were orally administered with the mixed herbal extract prepared in Example 3 at a dose of 40 ml / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities. As a result, there were no clinical symptoms or deaths in all animals treated with the test substance, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. As a result, all of the tested herbal extracts showed no toxic change in rats up to 40 ml / kg and the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance above 40 ml / kg.

As described above, the mixed herbal medicine extract of the present invention reduces the obesity inhibitory effect and high blood cholesterol and triglyceride by high fat diet, the extract can be used as a preventive or therapeutic aid for obesity according to high fat diet, etc. It can be usefully used as a weight loss agent or weight loss aid for beauty and the like.

1 is a graph showing the weight change of the normal diet group rats, high fat diet group rats (control) or high fat diet + rats administered the extract of the present invention,

■: normal diet group, ●: high-fat diet group (control),

▲: high fat diet + group administered the extract of the present invention,

Figure 2 is a graph showing the change in cholesterol of rats fed a normal diet group rat, high fat diet group rat (control) or high fat diet + the extract of the present invention,

CON: normal diet group, HF: high-fat diet group (control),

EX: high fat diet + group administered the extract of the present invention,

Figure 3 is a graph showing the change in HDL and LDL of the normal diet group rats, high-fat diet group rats (control) or high fat diet + rats administered the extract of the present invention,

CON: normal diet group, HF: high-fat diet group (control),

EX: high fat diet + group administered the extract of the present invention,

Figure 4 is a graph showing the change in triglycerides of the normal diet group rats, high fat diet group rats (control) or high fat diet + rats administered the extract of the present invention.

CON: normal diet group, HF: high-fat diet group (control),

EX: high fat diet + group administered the extract of the present invention

Claims (8)

i) preparing an extract by adding water or an aqueous alcohol solution to the herbal mixture of L. oleracea, green tea, tofu, garlic, hawthorn, raw pine needles and jinjin mugwort; And ii) a composition for preventing or treating obesity, which is obtained by further adding fermentation to rice, yeast and yeast to the extract of step i). The method according to claim 1, wherein in step i) the defector: green tea: tofu: garlic: hawthorn: green pine needles: jinjin 1-3: 0.5-1: 2-7: 1-3: 0.5-1: 3-7: 3 A composition characterized by mixing at a weight ratio (w / w) of -7. The composition of claim 1, wherein in step ii) rice: yeast: yeast is added at a weight ratio (w / w) of 20-80: 10-40: 0-2. The composition of claim 1 wherein the aqueous alcohol solution of step i) is selected from the group consisting of 5-100% ethyl alcohol and 5-100% methyl alcohol. The composition of claim 1, wherein the preparation of the extract of step i) is by hot water extraction. The composition of claim 1, wherein the fermentation process of step ii) is carried out at 15 to 30 ° C. for 5 to 15 days. A pharmaceutical composition for preventing or treating obesity, comprising the composition of claim 1 as an active ingredient. delete