CN104138402A - Drying method for eucommia leaves - Google Patents
Drying method for eucommia leaves Download PDFInfo
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- CN104138402A CN104138402A CN201410418716.3A CN201410418716A CN104138402A CN 104138402 A CN104138402 A CN 104138402A CN 201410418716 A CN201410418716 A CN 201410418716A CN 104138402 A CN104138402 A CN 104138402A
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- folium eucommiae
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- eucommia leaves
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- drying method
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Abstract
The invention relates to a drying method for eucommia leaves. The drying method is characterized by comprising the following steps of selecting fresh eucommia leaves; composting until the fresh eucommia leaves become brown; and drying to prepare the eucommia leaves. The content of various active components is remarkably increased, and the treating effect for hypertension is better.
Description
Invention field
The present invention relates to medicine method for making, particularly a kind of drying means of Folium Eucommiae, belongs to medical technical field.
Background technology
Traditional Chinese medicine drying has very long history in China, the Chinese herbal medicine of drying can keep certain medicinal ingredient and be difficult for putrid and deteriorated, the dry important measures as ensureing Chinese herbal medicine quality, it is a requisite technical process in Chinese herbal medicine processing, it is in close relations that dry and Chinese medicine are produced, and dry quality will directly affect the quality of product.
" Chinese Pharmacopoeia " (2010 editions one) record: Folium Eucommiae is the dried leaves of the Eucommiaceae plant Cortex Eucommiae.Summer, autumn gather when two seasons, branch and leaf were luxuriant, dry or oven drying at low temperature.We find that the dry Folium Eucommiae active constituent content of this method is low, and clinical effectiveness is poor.
Summary of the invention
The object of this invention is to provide a kind of drying means of Folium Eucommiae, the Folium Eucommiae that the method is dry, the drying means that plurality of active ingredients content records apparently higher than " Chinese Pharmacopoeia " (2010 editions one).
The object of the present invention is achieved like this:
A freeze drying process for Folium Eucommiae, is characterized in that: get fresh Folium Eucommiae, pile vexed to being brown, dry.
The application of Folium Eucommiae prepared by the drying means of a kind of Folium Eucommiae of the present invention, is characterized in that: be used for the treatment of hypertension.
So far completed the present invention, the Folium Eucommiae of preparation, various active component content is obviously high.Treatment hypertension better effects if.
Further illustrate usefulness of the present invention below by test example, test example is intended to further illustrate usefulness of the present invention, but not limitation of the present invention.
To, with a collection of fresh Folium Eucommiae, process by the following method.
1) dry Folium Eucommiae: get fresh Folium Eucommiae, dry.
2) pile the vexed Folium Eucommiae that dries: get fresh Folium Eucommiae, pile vexed 2 days, watch and be puce, dry.
One, the dry Folium Eucommiae active component comparison of distinct methods
1, key instrument and equipment
LC-20A high performance liquid chromatograph; UV-5300PC type ultraviolet-uisible spectrophotometer; AG135 type 100,000/electronic balance, freeze dryer.
2, reagent: reference substance chlorogenic acid, geniposide, rutin, caffeic acid are all purchased from Nat'l Pharmaceutical & Biological Products Control Institute, and Fructus Persicae skin Corallium Japonicum Kishinouye glycosides, pinoresinol diglucoside, Geniposidic acid provide by Shandong Traditional Chinese Medicine University.Acetonitrile (chromatographically pure, Tedia company of the U.S.); Phosphoric acid (chromatographically pure, Ke Miou chemical reagent development centre, Tianjin).
3, test method
Accurately take each sample appropriate, infiltrate after 12h with 60% ethanol, then use ultrasound wave assisted extraction 1h, be filtered in 100mL volumetric flask, repeat 2 times, merging filtrate, is settled to scale with 60% ethanol, and filtrate is crossed 45 μ m filter membranes, and extracting solution is for subsequent use.
1) high effective liquid chromatography for measuring chlorogenic acid chromatographic condition: chromatographic column Dimamonsil C18(250mm × 4.6mm, 5 μ are m); Mobile phase methanol: water=35: 75; Flow velocity 1.0mL/min detects wavelength 240nm; Sampling volume 20 μ L; 25 DEG C of temperature.
2) (250 mm x 4.6,5 μ m) for high effective liquid chromatography for measuring Geniposidic acid chromatographic condition: chromatographic column Dimamonsil C 18; Flow velocity 1.0 mL/min; Detect wavelength 240nm; Sampling volume 20 μ L; 25 DEG C of temperature; Binary geopressure gradient elution time program: when 0min, methanol: 0.001% phosphoric acid solution=22:78; When 15 min, methanol: 0.001% phosphoric acid solution=30:70; When 35 min, methanol: 0.001% phosphoric acid solution=22:78; When 40min, sampling finishes.
3) aluminum nitrate one sodium nitrite colorimetric method for determining total flavones is accurately drawn appropriate extracting solution in 10 mL volumetric flasks, adds containing 5%NaNO
2aqueous solution 0.3 mL, shakes up, and leaves standstill 6min; Add again (the N0 containing 10%Al
3)
3aqueous solution 0.3mL, shakes up, and leaves standstill 6min; Add again containing 1.0mol/L NaOH aqueous solution 4mL, shake up, leave standstill 15min; Be settled to scale with distilled water.In the interscan of 400-600nm wave-length coverage, determine that maximum absorption wavelength is 506nm.
4) paradime thylaminobenzaldehyde determination of color Fructus Persicae skin Corallium Japonicum Kishinouye glycosides is accurately drawn appropriate extracting solution in 10 mL color comparison tubes, adds successively 95% ethanol 2.5 mL, Epstahl reagent 1.7 mL, and 20% hydrochloric acid 0.5 mL, then be settled to scale with distilled water.Be placed in water-bath and at 75 DEG C, heat 20min and develop the color, take out after cooling 10min, taking reagent blank as reference, in the interscan of 500-700nm wave-length coverage, determine that maximum absorption wavelength is 596nm.
4, standard curve
Respectively chlorogenic acid, Geniposidic acid, total flavones and Fructus Persicae skin Corallium Japonicum Kishinouye glycosides standard specimen are measured drawing standard curve according to said method.
5, results and analysis
Adopt above-mentioned experimental technique to detect the content of various active composition in the Folium Eucommiae skin of distinct methods processing, result is as table 1.
Active constituent content result (%) in the dry Folium Eucommiae of table 1 distinct methods
Learnt by table 1, pile the content of vexed rear dry Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, Geniposidic acid, total flavones and aucubin apparently higher than drying Folium Eucommiae, and then known clinical efficacy is good.
Two, the dry Folium Eucommiae antihypertensive effect comparison of distinct methods
1, material
1.1 key instrument rat computer blood pressure cardiotachometers, four lead physiograph.
1.2 animal spontaneous hypertensive rats (SHR), are all male 10 week age, body weight (223.5 ± 23) g, and blood pressure is all greater than 20kPa.
1.3 prepare medicinal liquid gets respectively each sample, extracting in water 3 times, and amount of water is respectively 10,6,6 times of medical material weight, and extraction time is 1.5,1,1h, and merge extractive liquid,, concentrates and is settled to every milliliter and be equivalent to crude drug 0.25g, to obtain final product.
2, method and result
2.1 animal models are got rat feeding in constant temperature observation ward, room temperature (21 ± 2) DEG C, artificial lighting 12 hd-1, freely drink water, feeding with 21% high protein feed, measure blood pressure after 1 week, is spontaneous hypertensive rat higher than 22.7 kPa persons, it is divided at random and dries Folium Eucommiae group, pile vexed Folium Eucommiae group and the hypertension group of drying, 8 every group.Each medication group is respectively with 1.12gkg-1 gavage, every day 2 times, continuously 10d; 8 of normal rats, with hypertension group with commensurability distilled water gavage.
2h after the mensuration rat last administration of 2.2 blood pressures, heart rate, adopts the indirect measuring cell of non-invasive to measure arteria caudalis blood pressure, the heart rate of rat under waking state.Pressure measurement training every day 1 time before formal measurement, 5d altogether, conforms, after blood pressure stabilization, starts laboratory observation until rat.Before pressure measurement, rat is put into after (37 ± 1) DEG C electrothermostat preheating 15min, measure rat tail artery systolic pressure (SBP) and heart rate (HR), repeated measure 3 times.The dry Folium Eucommiae of distinct methods the results are shown in Table 2 to the impact of SHR blood pressure, heart rate.
The Folium Eucommiae antihypertensive effect that table 2 distinct methods is dry
| Grouping | Dosage (gkg -1) | SBP(kPa) | SR (inferior min -1) |
| Normal group | —— | 15.07±0.68 | 354±9.64 |
| Hypertension group | —— | 22.93±1.65 | 486±15.16 |
| Dry Folium Eucommiae | 1.12 | 19.16±1.10 | 441±14.15 |
| Pile the vexed Folium Eucommiae that dries | 1.12 | 16.82±1.14 | 359±12.41 |
From table 2, blood pressure and the heart rate of the dry Folium Eucommiae of 2 kinds of methods on laboratory animal has impact in various degree, wherein piles the vexed hypotensive effect that dries Folium Eucommiae group and is better than drying Folium Eucommiae group, illustrates that the dry Folium Eucommiae antihypertensive effect of the inventive method is good.
Detailed description of the invention
embodiment 1
Get fresh Folium Eucommiae, pile vexed 3 days, watch and be puce, dry.
embodiment 2
Get fresh Folium Eucommiae, pile vexedly to being brown, dry.
embodiment 3
Get fresh Folium Eucommiae, pile vexed to being brown, 60 DEG C of oven dry.
embodiment 4
Get fresh Folium Eucommiae, pile vexed 1 day, watch and be brown, dry.
embodiment 5
Get fresh Folium Eucommiae, pile vexed 3 days, watch and be brown, 60 DEG C of oven dry.
Claims (1)
1. a freeze drying process for Folium Eucommiae, is characterized in that: get fresh Folium Eucommiae, pile vexed to being brown, dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410418716.3A CN104138402A (en) | 2014-08-25 | 2014-08-25 | Drying method for eucommia leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410418716.3A CN104138402A (en) | 2014-08-25 | 2014-08-25 | Drying method for eucommia leaves |
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| CN201410418716.3A Pending CN104138402A (en) | 2014-08-25 | 2014-08-25 | Drying method for eucommia leaves |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1335178A (en) * | 2001-08-16 | 2002-02-13 | 王维新 | Gettama extract extracted from gettama leaf and its production process |
| KR20030085250A (en) * | 2002-04-29 | 2003-11-05 | (주) 김형민한약연구소 | Alcohol fermented food or pharmaceutical composition for prevention of obesity and process for preparation thereof |
-
2014
- 2014-08-25 CN CN201410418716.3A patent/CN104138402A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1335178A (en) * | 2001-08-16 | 2002-02-13 | 王维新 | Gettama extract extracted from gettama leaf and its production process |
| KR20030085250A (en) * | 2002-04-29 | 2003-11-05 | (주) 김형민한약연구소 | Alcohol fermented food or pharmaceutical composition for prevention of obesity and process for preparation thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘红亮等: "中药材"发汗"对药材质量的影响", 《中国实验方剂学杂志》 * |
| 赵中振等: "《中药材鉴定图典》", 30 April 2010 * |
| 饶伟文等: "中药产地加工规范化研究进展", 《中国中医药信息杂志》 * |
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Application publication date: 20141112 |