CN102872177A - Method for processing red ginseng - Google Patents
Method for processing red ginseng Download PDFInfo
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- CN102872177A CN102872177A CN2011101996105A CN201110199610A CN102872177A CN 102872177 A CN102872177 A CN 102872177A CN 2011101996105 A CN2011101996105 A CN 2011101996105A CN 201110199610 A CN201110199610 A CN 201110199610A CN 102872177 A CN102872177 A CN 102872177A
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Abstract
The invention provides a method for processing red ginseng. The method comprises the following steps of 1, slicing sun-dried ginseng; 2, performing acid steaming on the sun-dried ginsengs; and 3, drying. The extracts obtained by the method are high in content.
Description
Technical field:
The present invention relates to a kind of processing of crude drugs method, particularly a kind of red ginseng processing method.
Background technology:
Radix Ginseng Rubra is the dry root of Araliaceae Radix Ginseng Panax ginseng after steaming.As famous and precious traditional tonic Chinese medicine, have strongly invigorating primordial QI, multiple arteries and veins takes off admittedly, invigorating the spleen to benefit the lung, the effect such as promote the production of body fluid, calm the nerves.Radix Ginseng Rubra is usually used in weak body and prostration clinically, the cold extremities faint pulse, and insufficiency of the spleen lack of appetite, the deficiency of the lung is breathed with cough, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, and prolonged illness is empty wins, palpitation with fear insomnia, sexual impotence cold womb; Heart failure, cardiogenic shock.The Radix Ginseng Rubra polysaccharide is one of main active of Radix Ginseng Rubra, has the effect that strengthens function of immune system, immune antitumor and auxiliary antitumor, blood sugar lowering and regulation and control hemopoietic.
The ginsenoside Rg
3Be a kind of high activity rare ginsenoside that exists in the Radix Ginseng Rubra medical material, it has been subject to paying close attention to widely with its powerful antitumor activity.
The Radix Ginseng Rubra ginsenoside Rg of present processing method preparation
3Content is not satisfactory, and to the ginsenoside Rg
3Content destroy to some extent, therefore need a kind of Rg of new method processing high-load
3Radix Ginseng Rubra.
The invention provides a kind of new method and process the Rg of high-load
3Radix Ginseng Rubra.Providing simultaneously a kind of is the detection method of ensuring the quality of products relevant composition being carried out in the course of processing.May increase exponentially such as 5-HMF in the course of processing, and 5-HMF itself and catabolite thereof all may cause the generation of untoward reaction.So except Radix Ginseng total saponins and Rg
3Content outside, the content that increases 5 hydroxymethyl furfural (5-HMF) is investigated as common index.
Summary of the invention:
The purpose of this invention is to provide a kind of Rg3 Radix Ginseng Rubra of optimizing vinegar red ginseng processing technique processed and obtaining high-load.
The present invention adopts positive quadraturing design test, and Dichlorodiphenyl Acetate concentration, steaming time, bake out temperature and drying time carry out condition optimization, and the investigation index is Radix Ginseng total saponins, Rg
3Content and 5 hydroxymethyl furfural (HMF) content.The present invention has obtained following red ginseng processing method thus:
Step 1, the Radix Ginseng section
Step 2, the Radix Ginseng sheet steams with acid
Step 3, oven dry.
Wherein preferred method is:
Acetic acid is adopted in steaming with acid of step 2, and steaming time is 2-6 hour,
30-50 ℃ of lower baking 4-8 hour adopted in the oven dry of step 3.
Most preferred red ginseng processing method of the present invention is the section of step 1 Radix Ginseng, is to be cut into circular piece of uniform size, and thickness is 1-10mm, and after the Radix Ginseng section, the glacial acetic acid aqueous solution with 0.5% steams 4h, then 40 ℃ of lower baking 6h.
The present invention also comprises the quality determining method of passing judgment on simultaneously the Radix Ginseng Rubra quality with Rg3 and Total Ginsenosides Content as index.
Of the present inventionly pass judgment on simultaneously the detection method of Radix Ginseng Rubra quality as index with Rg3 and Total Ginsenosides Content, may further comprise the steps:
Step 1, the preparation of need testing solution
Get Red Ginseng 5g, accurately weighed, after the adding chloroform 50ml backflow defat, add 85% alcohol reflux 2 times, each 40ml, 2h, 1h reflux respectively.Extracting solution merges and to be concentrated into without the alcohol flavor, is settled in the 25ml measuring bottle with 20% methanol solution transfer of 0.5molL-1 sodium hydroxide.Precision is measured mentioned solution 10ml, in advance with the good DIKMAProElut of solvent processing
TMThe SPE pillar, with 20% methanol solution 5ml and the 20% methanol solution 10ml flushing of the sodium hydroxide of 0.5molL-1, effluent discards, be collected in extremely nearly scale in the 10ml measuring bottle with methanol-eluted fractions at last, add methanol to scale, shake up, cross 0.45 μ m filter membrane, as need testing solution.
Step 2, the reference substance solution preparation
Precision takes by weighing ginsenoside Re's reference substance, adds methanol and makes the solution that every 1ml contains 1.736mg, shakes up, and get final product.
Precision takes by weighing the 20S-ginsenoside Rg
3And 20R-ginsenoside Rg
3Reference substance is an amount of, adds methanol and makes respectively every 1ml and contain the 20S-ginsenoside Rg
30.400mg and 20R-ginsenoside Rg
30.312mg mixed mark liquid.With Rg
3Mixed mark liquid dilutes respectively 2,4,8,40 times, and standardize solution is for subsequent use.
Step 3, total saponin content is measured
Precision is measured Re reference substance solution 20,40,60,80, and 100 μ l and need testing solution 40 μ l put respectively in the 10ml tool plug test tube.Put and take out after waving most solvent in the water-bath, let cool, precision adds 5% vanillin of new preparation-glacial acetic acid and mixed liquid (2: 8) 1ml of perchloric acid, shakes up.Put in 60 ℃ of water-baths and heat 15min, take out, put immediately and cool off 2min in the ice bath.Precision adds glacial acetic acid 5ml, shakes up, and at room temperature places 5min.Take corresponding reagent as blank, measure trap at the 550nm place, calculate, and get final product.
Step 4, Rg
3The mensuration of content
Waters
C18 (250 * 4.6mm, 5 μ m) chromatographic column; Take acetonitrile and 0.05% phosphoric acid solution as mobile phase, carry out gradient elution by table 2; The detection wavelength is 203nm; Column temperature is 30 ℃; Flow velocity 1.0mlmin-1; Limit of integration 8～70min; Number of theoretical plate calculates by 20 (S)-ginsenoside Rg3 peaks should be not less than 6000.Get respectively each 10 μ L of reference substance solution and need testing solution, injecting chromatograph is measured.The present invention also comprises the quality determining method of passing judgment on the Radix Ginseng Rubra quality with 5-HMF content as index.Of the present invention with the Radix Ginseng Rubra quality determining method of 5-HMF content as index, may further comprise the steps:
Step 1, the preparation of need testing solution
Get Radix Ginseng Rubra medicinal powder 5g, accurately weighed, put in the 100ml tool plug conical flask, add 50ml methanol, soaked overnight, supersound process (power 250W, frequency 50kHZ) 30min, filter, filtering residue is measured the methanol supersound extraction 2 times, merge extractive liquid, with 10 times again, be concentrated into 25ml, cross microporous filter membrane, get subsequent filtrate, as need testing solution.
Step 2, the preparation of reference substance solution
It is an amount of to get the 5-HMF reference substance, accurately weighed, makes the reference substance solution that every 1ml contains respectively 0.695 μ g, 2.75 μ g, 6.95 μ g, 1.39 μ g and 4.17 μ g with methanol.
Step 3, chromatographic condition and mensuration
Waters
C18 (250 * 4.6mm, 5 μ m) chromatographic column; Take methanol-water as mobile phase, gradient elution (seeing Table 3), flow velocity 1.0mlmin-1; Column temperature is 30 ℃; The detection wavelength is 284nm; Number of theoretical plate calculates by the 5-HMF peak should be not less than 6000.Precision is drawn reference substance solution and each 10 μ l of need testing solution respectively, and the injection liquid chromatography is measured, and be get final product.
Technical scheme of the present invention obtains through screening, and screening process is as follows:
1 material and instrument
3 years living dry and whole RADIX GINSENGs (Fusong product) are identified through professor Guo Qiaosheng of Agricultural University Of Nanjing, are Radix Ginseng Panax ginseng C.A.Mey.Lower palpus, section, stand-by.
Radix Ginseng Rubra control medicinal material, ginsenoside Re and 5 hydroxymethyl furfural reference substance are that Nat'l Pharmaceutical ﹠ Biological Products Control Institute buys; The 20S-ginsenoside Rg
3With the 20R-ginsenoside Rg
3Reference substance is bought from Jilin University.
Waters 2695 high performance liquid chromatographs, 2489 UV-detector; Hach DR 5000 uv-spectrophotometric instrument.
2 orthogonal test methods
Adopt positive quadraturing design test, investigate acetate concentration, steaming time, bake out temperature, four conditions of drying time
[i](concrete level sees Table 1).The investigation index is Total Ginsenosides Content, ginsenoside Rg
3Content and 5-HMF content.Experiment is total to get nine kinds of Radix Ginseng Rubra samples, beats respectively powder, crosses sieve No. four, is sealed in the valve bag, is sample 1～9.
Table 1 factor level table
Because it is too much to investigate index, so to Total Ginsenosides Content and ginsenoside Rg
3Content adopts comprehensive grading
[ii], as experimental result 1,5-HMF content is as experimental result 2 with relative value's sum (being called " saponin value ")." saponin value " calculated according to following formula.
3 Radix Ginseng total saponinss and Rg
3The mensuration of content
[iii]
3.1 the preparation of need testing solution
Get Red Ginseng 5g, accurately weighed, after the adding chloroform 50ml backflow defat, add 85% alcohol reflux 2 times, each 40ml, 2h, 1h reflux respectively.Extracting solution merges and to be concentrated into without the alcohol flavor, is settled in the 25ml measuring bottle with 20% methanol solution transfer of 0.5molL-1 sodium hydroxide.Precision is measured mentioned solution 10ml, in advance with the good DIKMAProElut of solvent processing
TMThe SPE pillar, with 20% methanol solution 5ml and the 20% methanol solution 10ml flushing of the sodium hydroxide of 0.5molL-1, effluent discards, be collected in extremely nearly scale in the 10ml measuring bottle with methanol-eluted fractions at last, add methanol to scale, shake up, cross 0.45 μ m filter membrane, as need testing solution.
3.2 reference substance solution preparation
Precision takes by weighing ginsenoside Re's reference substance, adds methanol and makes the solution that every 1ml contains 1.736mg, shakes up, and get final product.
Precision takes by weighing the 20S-ginsenoside Rg
3And 20R-ginsenoside Rg
3Reference substance is an amount of, adds methanol and makes respectively every 1ml and contain the 20S-ginsenoside Rg
30.400mg and 20R-ginsenoside Rg
30.312mg mixed mark liquid.With Rg
3Mixed mark liquid dilutes respectively 2,4,8,40 times, and standardize solution is for subsequent use.
3.3 total saponin content is measured
Precision is measured Re reference substance solution 20,40,60,80, and 100 μ l and need testing solution 40 μ l put respectively in the 10ml tool plug test tube.Put and take out after waving most solvent in the water-bath, let cool, precision adds 5% vanillin of new preparation-glacial acetic acid and mixed liquid (2: 8) 1ml of perchloric acid, shakes up.Put in 60 ℃ of water-baths and heat 15min, take out, put immediately and cool off 2min in the ice bath.Precision adds glacial acetic acid 5ml, shakes up, and at room temperature places 5min.Take corresponding reagent as blank, measure trap at the 550nm place, calculate, and get final product.
3.4Rg
3The mensuration of content
Waters
C18 (250 * 4.6mm, 5 μ m) chromatographic column; Take acetonitrile and 0.05% phosphoric acid solution as mobile phase, carry out gradient elution by table 2; The detection wavelength is 203nm; Column temperature is 30 ℃; Flow velocity 1.0mlmin-1; Limit of integration 8～70min; Number of theoretical plate calculates by 20 (S)-ginsenoside Rg3 peaks should be not less than 6000.Get respectively each 10 μ L of reference substance solution and need testing solution, injecting chromatograph is measured.
Table 2HPLC measures Rg
3The concentration gradients elution program
The mensuration of 45-HMF content
4.1 the preparation of need testing solution
Get Radix Ginseng Rubra medicinal powder 5g, accurately weighed, put in the 100ml tool plug conical flask, add 50ml methanol, soaked overnight, supersound process (power 250W, frequency 50kHZ) 30min, filter, filtering residue is measured the methanol supersound extraction 2 times, merge extractive liquid, with 10 times again, be concentrated into 25ml, cross microporous filter membrane, get subsequent filtrate, as need testing solution.
4.2 the preparation of reference substance solution
It is an amount of to get the 5-HMF reference substance, accurately weighed, makes the reference substance solution that every 1ml contains respectively 0.695 μ g, 2.75 μ g, 6.95 μ g, 1.39 μ g and 4.17 μ g with methanol.
4.3 chromatographic condition and mensuration
Waters
C18 (250 * 4.6mm, 5 μ m) chromatographic column; Take methanol-water as mobile phase, gradient elution (seeing Table 3), flow velocity 1.0mlmin-1; Column temperature is 30 ℃; The detection wavelength is 284nm; Number of theoretical plate calculates by the 5-HMF peak should be not less than 6000.Precision is drawn reference substance solution and each 10 μ l of need testing solution respectively, and the injection liquid chromatography is measured, and be get final product.
Table 3HPLC measures 5-HMF concentration gradients elution program
4.4 methodological study
Use the 5-HMF content in the method mensuration Radix Ginseng Rubra medical material, standard curve is y=80880x-45845, (0.695≤x≤6.95 μ g/g), r=0.9997; Instrument precision (n=5) RSD is 0.39%; Repeatability (n=6) RSD is 2.26%; Sample is 2.22% at 24h internal stability RSD; All between 95.28%～101.68%, RSD 2.13% for average recovery (n=6).The method accurate stable is described, can be used for measuring the content of 5-HMF in the medical material.
5 experimental results
5.1 Radix Ginseng Rubra sample survey result
Calculate respectively 20S-ginsenoside Rg in the Radix Ginseng Rubra medical material
3With the 20R-ginsenoside Rg
3Content, Total Ginsenosides Content, 5-HMF content (calculating with dry product).The formula that recycles 2.1 calculates " saponin value ", result such as table 4.
Table 4 vinegar Radix Ginseng Rubra orthogonal test processed sample survey result
5.2 orthogonal experiments
We respectively with " saponin value " and 5-HMF content as index, estimate the result of orthogonal test.Specifically see the following form.
Table 5 orthogonal experiments 1---" saponin value "
Table 6 orthogonal experiments 2---5-HMF
6 analyze and discuss
6.1 as shown in Table 5, factor A and B have appreciable impact (P＜0.05) to " saponin value ".It is A＞B＞D＞C that each factor is closed the primary and secondary of saponin value impact.From average, each level of factor A sequentially is K
3≈ K
2＞K
1, illustrate that acetate concentration effect 0.5% and 1% time is better.Each level of factor B sequentially is K
3＞K
2＞K
1, illustrating that steaming time is longer, saponin content is higher.Each level of factor C sequentially is K
1≈ K
2＞K
3, showing oven dry in the time of 2～4 hours, saponin content is higher.Each level of factor D sequentially is K
1＞K
3＞K
2, show that when 40 ℃ of lower oven dry saponin content is the highest in the Radix Ginseng Rubra medical material.
6.2 as shown in Table 6, factor A and B have appreciable impact (P＜0.05) to 5-HMF content, and each factor the primary and secondary of 5-HMF content influence is closed is A＞B＞C＞D.From average, each level of factor A and C sequentially is K
3＞K
2＞K
1, illustrating that acetate concentration is larger, steaming time is longer, and the content of 5-HMF is larger.But compare three levels, find that the content influence of acetate concentration 0.5% and 0.1% couple of 5-HMF is little, but when acetate concentration is 1%, the content of 5-HMF is 0.5% nearly three times in the Radix Ginseng Rubra.Similarly, steam 4 hours and 2 hours little to the content influence of 5-HMF, but when steaming 6 hours, the content of 5-HMF is significantly increased in the Radix Ginseng Rubra.By factor C and D result as can be known, drying time is longer, and temperature is higher, and the content of 5-HMF is lower.But impact is not very large.
6.3 consider that the Radix Ginseng sheet steams after bake and just can thoroughly dry in 4～6 hours, take again 6.1 and 6.2 into consideration, we finally select optimum processing scheme is A
2B
2C
2D
1, i.e. after the Radix Ginseng section, the glacial acetic acid aqueous solution with 0.5% steams 4h, then 40 ℃ of lower baking 6h.
7 technology stability demonstration tests
According to the optimum process condition in the orthogonal test, the Radix Ginseng sheet is processed.Triplicate obtains three batches of vinegar Radix Ginseng Rubra medical materials processed, is respectively 1101P, 1102P and 1103P.Not add Radix Ginseng Rubra that acetic acid steams in contrast, measure its ginsenoside Rg
3Content, total saponin content and 5-HMF content.Result such as following table.
Table 4-16 vinegar Radix Ginseng Rubra technology stability processed experimental result
Three crowdes of demonstration test results show that this process stabilizing is feasible, and the Rg of contrast Radix Ginseng Rubra
3Content is significantly improved.
The specific embodiment:
Further specify by the following examples the present invention, but not as limitation of the present invention.
Embodiment 1, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 4h with 0.5% glacial acetic acid aqueous solution,
Step 3, the Radix Ginseng sheet after steaming is at 40 ℃ of lower baking 6h.
Embodiment 2, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 4h with 0.5% aqueous hydrochloric acid solution,
Step 3, the Radix Ginseng sheet after steaming is at 40 ℃ of lower baking 6h.
Embodiment 3, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 4h with 0.5% aqueous sulfuric acid,
Step 3, the Radix Ginseng sheet after steaming is at 40 ℃ of lower baking 6h.
Embodiment 4, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 4h with 0.5% lactic acid aqueous solution,
Step 3, the Radix Ginseng sheet after steaming is at 40 ℃ of lower baking 6h.
Embodiment 5, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 2h with 0.5% glacial acetic acid aqueous solution,
Step 3, the Radix Ginseng sheet after steaming is at 30 ℃ of lower baking 4h.
Embodiment 6, the preparation of Radix Ginseng Rubra
Step 1, circular piece of uniform size is cut in the Radix Ginseng section, and thickness is 1-10mm,
Step 2, the Radix Ginseng sheet steams 6h with 0.5% glacial acetic acid aqueous solution,
Step 3, the Radix Ginseng sheet after steaming is at 50 ℃ of lower baking 8h.
Claims (5)
1. the preparation method of a Radix Ginseng Rubra is characterized in that, the process following steps:
Step 1, the Radix Ginseng section,
Step 2, the Radix Ginseng sheet steams with acid,
Step 3, oven dry.
2. according to claim 1 preparation method is characterized in that, wherein, acetic acid is adopted in steaming with acid of step 2, and steaming time is 2-6 hour, and 30-50 ℃ of lower baking 4-8 hour adopted in the oven dry of step 3.
3. according to claim 1 preparation method is characterized in that, wherein step 1 Radix Ginseng section, to be cut into circular piece of uniform size, thickness is 1-10mm, and the glacial acetic acid aqueous solution with acid steaming employing 0.5% of step 2 steams 4h, and the baking of step 3 is at 40 ℃ of lower baking 6h.
4. pass judgment on simultaneously the quality determining method of Radix Ginseng Rubra quality as index with Rg3 and Total Ginsenosides Content for one kind, it is characterized in that, may further comprise the steps:
Step 1, the preparation of need testing solution
Get Red Ginseng 5g, accurately weighed, after adding chloroform 50ml backflow defat, add 85% alcohol reflux 2 times, each 40ml, 2h, 1h reflux respectively, extracting solution merges to be concentrated into without alcohol distinguishes the flavor of, shift to be settled in the 25ml measuring bottle with 20% methanol solution of 0.5molL-1 sodium hydroxide, precision is measured mentioned solution 10ml, in advance with the good DIKMA ProElut of solvent processing
TMThe SPE pillar, with 20% methanol solution 5ml and the 20% methanol solution 10ml flushing of the sodium hydroxide of 0.5molL-1, effluent discards, be collected in extremely nearly scale in the 10ml measuring bottle with methanol-eluted fractions at last, add methanol to scale, shake up, cross 0.45 μ m filter membrane, as need testing solution;
Step 2, the reference substance solution preparation
Precision takes by weighing ginsenoside Re's reference substance, adds methanol and makes the solution that every 1ml contains 1.736mg, shakes up, and get final product;
Precision takes by weighing the 20S-ginsenoside Rg
3And 20R-ginsenoside Rg
3Reference substance is an amount of, adds methanol and makes respectively every 1ml and contain the 20S-ginsenoside Rg
30.400mg and 20R-ginsenoside Rg
30.312mg mixed mark liquid, with Rg
3Mixed mark liquid dilutes respectively 2,4,8,40 times, and standardize solution is for subsequent use;
Step 3, total saponin content is measured
Precision is measured Re reference substance solution 20,40,60,80,100 μ l and need testing solution 40 μ l put respectively in the 10ml tool plug test tube, put and take out after waving most solvent in the water-bath, let cool, precision adds 5% vanillin of new preparation-glacial acetic acid and the mixed liquid of perchloric acid=2: 81ml, shakes up, and puts in 60 ℃ of water-baths and heats 15min, take out, put immediately and cool off 2min in the ice bath, precision adds glacial acetic acid 5ml, shake up, at room temperature place 5min, take corresponding reagent as blank, measure trap at the 550nm place, calculate, and get final product;
Step 4, Rg
3The mensuration of content
250 * 4.6mm, the Waters of 5 μ m
The C18 chromatographic column; Take acetonitrile and 0.05% phosphoric acid solution as mobile phase, carry out gradient elution by table 2; The detection wavelength is 203nm; Column temperature is 30 ℃; Flow velocity 1.0mlmin-1; Limit of integration 8～70min; Number of theoretical plate calculates by 20 (S)-ginsenoside Rg3 peaks should be not less than 6000, gets respectively each 10 μ L of reference substance solution and need testing solution, and injecting chromatograph is measured.
5. pass judgment on the quality determining method of Radix Ginseng Rubra quality with 5-HMF content as index for one kind, it is characterized in that, may further comprise the steps:
Step 1, the preparation of need testing solution
Get Radix Ginseng Rubra medicinal powder 5g, accurately weighed, put in the 100ml tool plug conical flask, add 50ml methanol, soaked overnight, power 250W, frequency 50kHZ supersound process 30min filters, and filtering residue is measured the methanol supersound extraction 2 times with 10 times again, merge extractive liquid,, be concentrated into 25ml, cross microporous filter membrane, get subsequent filtrate, as need testing solution
Step 2, the preparation of reference substance solution
It is an amount of to get the 5-HMF reference substance, accurately weighed, makes the reference substance solution that every 1ml contains respectively 0.695 μ g, 2.75 μ g, 6.95 μ g, 1.39 μ g and 4.17 μ g with methanol,
Step 3, chromatographic condition and mensuration
250 * 4.6mm, the Waters of 5 μ m
The C18 chromatographic column; Take methanol-water as mobile phase, gradient elution, flow velocity 1.0mlmin-1; Column temperature is 30 ℃; The detection wavelength is 284nm; Number of theoretical plate calculates by the 5-HMF peak should be not less than 6000, and precision is drawn reference substance solution and each 10 μ l of need testing solution respectively, and the injection liquid chromatography is measured, and be get final product.
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| CN105535053A (en) * | 2016-01-14 | 2016-05-04 | 吉林大学 | Preparation method of radix ginseng rubra paste |
| CN106236802A (en) * | 2016-07-25 | 2016-12-21 | 天津宜诺医药工业设计有限公司 | A kind of improve the method for Radix Ginseng total saponins after red ginseng processing |
| CN106387260A (en) * | 2016-09-08 | 2017-02-15 | 天津天元极生物科技有限公司 | Processing method of chewy ready-to-eat radix ginseng rubra |
| CN109793767A (en) * | 2017-11-17 | 2019-05-24 | 牡丹江医学院 | A kind of red ginseng manufacture craft promoting content of ginsenoside |
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| CN105535053A (en) * | 2016-01-14 | 2016-05-04 | 吉林大学 | Preparation method of radix ginseng rubra paste |
| CN105535053B (en) * | 2016-01-14 | 2019-06-21 | 吉林大学 | A kind of preparation method of red ginseng cream |
| CN106236802A (en) * | 2016-07-25 | 2016-12-21 | 天津宜诺医药工业设计有限公司 | A kind of improve the method for Radix Ginseng total saponins after red ginseng processing |
| CN106387260A (en) * | 2016-09-08 | 2017-02-15 | 天津天元极生物科技有限公司 | Processing method of chewy ready-to-eat radix ginseng rubra |
| CN106387260B (en) * | 2016-09-08 | 2020-01-24 | 江西康一参生物科技有限公司 | Processing method of Q pellet instant red ginseng slices |
| CN109793767A (en) * | 2017-11-17 | 2019-05-24 | 牡丹江医学院 | A kind of red ginseng manufacture craft promoting content of ginsenoside |
| CN109793767B (en) * | 2017-11-17 | 2021-07-16 | 牡丹江医学院 | Ginseng radix Rubri preparation process capable of increasing ginsenoside content |
| CN109932432A (en) * | 2017-12-15 | 2019-06-25 | 富力 | The method of quality control of Shenyi capsule bulk pharmaceutical chemicals all the period of time multi-wavelength fusion finger-print |
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