CN106138141A - A kind of cooked Radix Notoginseng and preparation method thereof - Google Patents
A kind of cooked Radix Notoginseng and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of cooked Radix Notoginseng, according to the percentage calculation of its weight, total saponin content is 6.0 12.0%, and the content sum of the rudimentary property saponin being wherein converted into accounts for the 40 90% of cooked Radix Notoginseng total saponins, and the intrinsic saponin of Radix Notoginseng includes Panax Notoginseng saponin R1, ginsenoside Rg1, ginsenoside Rb1, the low polarity saponin being converted into includes ginsenoside 20(S) and Rh1, ginsenoside 20(R) Rh1, ginsenoside Rd, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20(S) Rg3, ginsenoside 20(R) Rg3, ginsenoside Rk1, ginsenoside Rg5.The invention also discloses the preparation method of cooked Radix Notoginseng, obtaining a kind of cooked Radix Notoginseng with specific saponin and ratio composition thereof by accurately controlling amount of water, process of preparing Chinese medicine temperature and concocted time, it has raising immunologic function and the low polarity saponin active component of antitumor action rich in there's almost no in raw sangqi ginseng.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of cooked Radix Notoginseng and preparation method thereof.
Background technology
Radix Notoginseng, another name: Panax pseudoginseng Wall., Panax pseudoginseng, Typhonium flagelliforme (Lodd.) Blume, mountain paint, Radix Notoginseng, Classification system: Panax
Notoginseng (Burk.) F.H.Chen, for Araliaceae Panax herbaceos perennial, root stock is short, fleshy tap root cylinder
Shape, is distributed in the ground such as Yunnan, Guangxi, Jiangxi, Sichuan.Radix Notoginseng is mainly rich in saponin, flavone, Argine Monohydrochloride and non-protein amino
The compositions such as acid, polysaccharide, albumen, sterol, inorganic matter, volatile oil and oils and fats.The principle active component of Radix Notoginseng is arasaponin, so far
Till, plant monomer saponin composition from Radix Notoginseng different parts isolated more than 60, these monomer saponin composition great majority are for reaching agate
20 (the S)-protopanaxadiol-type [20 (S)-protopanaxadio1] of alkane and 20 (S)-Protopanaxatriol types [20 (S)-
Peotopanaxatrio1], but do not find containing oleanolic acid type saponin.This has significantly with congener Radix Ginseng and Radix Panacis Quinquefolii
Difference.
According to the difference of concocting method, the mitogenetic Radix Notoginseng of notoginseng decoction piece, cooked Radix Notoginseng.Raw sangqi ginseng is its intrinsic soap after high temperature is concocted
The configuration of glycosidic bond generation cracking reaction, side chain dehydration and the C-20 of glycosides changes and generates new saponin, i.e. Radix Notoginseng soap
Glycosides R1, ginsenoside Rg1, ginsenoside Rb1The content of 3 kinds of monomer saponins is gradually lowered, and newly-generated ginsenoside 20 (S)-
Rh1, ginsenoside 20 (R)-Rh1, ginsenoside Rd, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3, Radix Ginseng
Saponin 20 (R)-Rg3, ginsenoside Rk1, ginsenoside Rg5Content be stepped up.Aqueous by Radix Notoginseng of shifting degree
Measuring and concoct temperature and time correlation, the percentage composition of the low polarity saponin being converted into changes between zero to 100%,
This creates from raw sangqi ginseng to the cooked Radix Notoginseng product of full ripe numerous differing maturities, the cooked Radix Notoginseng that these Maturity are different
Because material base is different, there is different effects.Radix Notoginseng the most just has saying of " the raw ripe benefit that disappears ", and raw sangqi ginseng only has scattered silt
Blood, subduing swelling and relieving pain effect, it is mainly used in spitting of blood, spits blood, have blood in stool, metrorrhagia, traumatic hemorrhage, chest and abdomen are sharp, tumbling and swelling.Cooked Radix Notoginseng
Being longer than nourishing, Radix Notoginseng can relax the haemostatic properties of Radix Notoginseng through concocting, strengthen Radix Notoginseng enrich blood, the curative effect of QI invigorating, have antiinflammatory,
Defying age, antitumor, improve the function of immunity of organism, and the highest benefiting action of Maturity is the strongest.
In the many factors affecting cooked Radix Notoginseng Maturity, the water content of Radix Notoginseng and the temperature and time of the process of preparing Chinese medicine are to determine
The big key factor of the three of cooked Radix Notoginseng Maturity, directly decides saponin kind and the change of content in cooked Radix Notoginseng.Along with amount of water
Increase, temperature raises and time lengthening, the intrinsic saponin such as Panax Notoginseng saponin R in raw sangqi ginseng1, ginsenoside Rg1, ginsenoside Rb1
To persistently be cracked conversion and be gradually lowered, be progressively down to the extremely low water close to zero from raw sangqi ginseng weight percentage more than 5%
Flat, and crack low polarity saponin ginsenoside 20 (the S)-Rh being converted into1, ginsenoside 20 (R)-Rh1, ginsenoside Rd, people
Ginseng saponin Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3, ginsenoside 20 (R)-Rg3, ginsenoside Rk1, ginsenoside
Rg5The most progressively increasing, extremely low value sustainable growth almost nil from raw sangqi ginseng is to more than the 5% of cooked Radix Notoginseng weight.Thus may be used
See that cooked Radix Notoginseng prepared by different amount of water, steaming temperature and ageings is the product that inherent quality is different, accurately control to steam
The technical parameter boiling technique is the essential condition obtaining the cooked Radix Notoginseng with specific low polarity saponin kind and content.Low polarity people
Ginseng saponin Rg3As a kind new medicine approval listing, this product is applicable to the treatment of tumor, has significantly suppression tumor thin
Born of the same parents' proliferation function.And ginsenoside Rh1, ginsenoside Rh4, ginsenoside Rk3, ginsenoside Rg3, ginsenoside Rg5, Radix Ginseng soap
Glycosides Rk1Immune function of human body and anti-tumor activity, the kind of these low polarity saponins and content is improved Deng also having been demonstrated to have
Difference determine the difference of cooked Radix Notoginseng curative effect.
Traditional concocting method of Radix Notoginseng is clean system, baking or dry in the sun, profit cutting or pulverizing, makes raw sangqi ginseng, raw sangqi ginseng sheet
With raw sangqi ginseng powder.Cooked Radix Notoginseng of the prior art is also to obtain by steaming, and such as Chinese patent CN200910113914.8 is public
Open a kind of Radix Notoginseng processing method and products thereof, it is characterised in that take the root and rhizome of Radix Notoginseng, be first processed into raw sangqi ginseng or run through
Radix Notoginseng；Or raw sangqi ginseng sheet, the Radix Notoginseng Tabellae that runs through；Or Radix Notoginseng powder；Put high temperature 106～125 DEG C, high pressure in flowing steam pot again
0.03～0.14MPa steams 1～15 hour, to inside and outside in sepia, takes out, is dried, to obtain final product.Use prepared by this technique ripe three
Seven, wherein Radix Notoginseng total arasaponins is from the most unconverted to converting the most likely, i.e. concocts in the range of its different technology conditions
The different cooked Radix Notoginseng of Maturity become, its inherent quality has the difference of essence, and from this patent specification accompanying drawing
HPLC chromatogram be can't see low polarity saponin ginsenoside 20 (the S)-Rh being converted at all1, ginsenoside 20 (R)-Rh1、
Ginsenoside Rd, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3, ginsenoside 20 (R)-Rg3, Radix Ginseng soap
Glycosides Rk1, ginsenoside Rg5Chromatographic peak.And the patent of invention of Application No. 201510023537.4 disclose a kind of cooked Radix Notoginseng and
Preparation Method And The Use, this cooked Radix Notoginseng is based on percentage by weight, containing Panax Notoginseng saponin R1, ginsenoside Rg1, ginsenoside Rb1
The total amount of three must not be less than 5.0%, wherein Panax Notoginseng saponin R1Content must not be less than 0.4%, ginsenoside Rg1Content not
Must be less than 2.0%, ginsenoside Rb1Content must not be less than 1.5%；Containing ginsenoside Rh4, ginsenoside 20 (S)-Rg3、
Ginsenoside 20 (R)-Rg3The total amount of three must not be less than 0.5%, wherein ginsenoside Rh4Content must not be less than 0.3%, Radix Ginseng
Saponin 20 (S)-Rg3Content is no less than 0.06%, ginsenoside 20 (R)-Rg3Content is no less than 0.03%.This patent disclosed that
A kind of low-down cooked Radix Notoginseng of transforming degree.Arasaponin in Pharmacopoeia of People's Republic of China 2015 editions regulation raw sangqi ginseng
R1, ginsenoside Rg1, ginsenoside Rb1Total content must not be less than 5.0%, i.e. according to preparation technology disclosed in this patent of invention
The cooked Radix Notoginseng of preparation still conforms to the standards of pharmacopoeia of raw sangqi ginseng, Panax Notoginseng saponin R therein1, ginsenoside Rg1, ginsenoside Rb1
The most unconverted, and the low polarity saponin content being converted into is the lowest, the proportion of composing of its saponin constituent is complete with this patent
Inconsistent, illustrate that above-mentioned patent is unrelated with this patent.
Summary of the invention
An object of the present invention is to provide a kind of cooked Radix Notoginseng, be provided in particular in a kind of disappear mend in a basic balance have specific
The cooked Radix Notoginseng of saponin composition.
An object of the present invention is to provide the new processing procedure of a kind of cooked Radix Notoginseng.
The cooked Radix Notoginseng that the present invention provides, has techniques below feature: i.e. based on its percentage by weight meter, this cooked Radix Notoginseng contains
Total saponins is 6.0-12.0%, and the content sum of the 9 kinds of rudimentary property saponin being wherein converted into accounts for the 40-of cooked Radix Notoginseng total saponins
90%, preferably 50-80%.The intrinsic saponin of Radix Notoginseng includes Panax Notoginseng saponin R1, ginsenoside Rg1, ginsenoside Rb1, three's total content
Less than 5.0%, each monomer saponin content is successively less than 0.01-0.5%, 0.2-2.0% and 0.3-3.0%；Nine be converted into
Individual low polarity saponin ginsenoside 20 (S)-Rh1, ginsenoside 20 (R)-Rh1, ginsenoside Rd, ginsenoside Rk3, Radix Ginseng soap
Glycosides Rh4, ginsenoside 20 (S)-Rg3, ginsenoside 20 (R)-Rg3, ginsenoside Rk1, ginsenoside Rg5Content be followed successively by
0.3-1.5%, 0.1-1.0%, 0.05-1.0%, 0.2-1.5%, 0.5-3.0%, 0.1-1.0%, 0.05-0.5%, 0.1-
1.0%, 0.5-3.0%.
The preparation method of the cooked Radix Notoginseng that the present invention provides is as follows:
1) clean: take Radix Notoginseng dry product, carry out prewashing with water, wash away the earth being attached to Radix Notoginseng surface, add Radix Notoginseng dry product weight
The drinking water of amount 0.5～2 times amount is dipped to water and blots, then with drinking water, Radix Notoginseng is carried out fine purifiation, thorough eccysis Radix Notoginseng surface
Earth and impurity, must moisten Radix Notoginseng；Or take Radix Notoginseng fresh goods, earth and the impurity removing Radix Notoginseng surface is thoroughly cleaned with drinking water,
Profit Radix Notoginseng；
2) pretreatment: take profit Radix Notoginseng, dry, pulverize, obtain raw sangqi ginseng powder, add raw sangqi ginseng grain weight amount 0.5-2 times weight
Drinking water, infiltration, to the saturating heart, obtains profit Radix Notoginseng powder；Or take profit Radix Notoginseng, cut into the sheet of 1-3 millimeter, obtain profit Radix Notoginseng Tabellae；
3) steam: taking profit Radix Notoginseng, profit Radix Notoginseng Tabellae or profit Radix Notoginseng powder, be laid in charging tray, thickness is 1-5 centimetre, puts pressure
In Guo, steam 1-4 hour in 116-125 DEG C；
4) it is dried: dry 2-24 hour in 50-105 DEG C, obtains cooked Radix Notoginseng, cooked Radix Notoginseng sheet or cooked Radix Notoginseng powder；
5) pulverize: pulverize, obtain cooked Radix Notoginseng powder；Or pulverize, comminution by gas stream, obtain cooked Radix Notoginseng micropowder.
Step 1) described in Radix Notoginseng dry product or fresh Radix Notoginseng raw material be the main root of araliaceae ginseng plant Radix Notoginseng, supporting root,
One or more in rhizome, preferably rhizomes of Panax notoginseng；Step 3) in profit Radix Notoginseng, profit Radix Notoginseng Tabellae or profit Radix Notoginseng powder to steam condition excellent
Elect temperature 118-122 DEG C, 2-3 hour time as；Step 4) described in drying means be constant pressure and dry or vacuum drying.
The present invention obtains, by the accurately control of amount of water, process of preparing Chinese medicine temperature and time, the cooked Radix Notoginseng that a kind of degree of depth is concocted,
It has raising immunologic function and the low polarity saponin active component of antitumor action rich in there's almost no in raw sangqi ginseng.
Accompanying drawing explanation
Fig. 1 is the HPLC chromatogram of mixing reference substance, wherein 1: Panax Notoginseng saponin R1, 2: ginsenoside Rg1, 3: ginsenoside
Rb1, 4: ginsenoside 20 (S)-Rh1, 5: ginsenoside 20 (R)-Rh1, 6: ginsenoside Rd, 7: ginsenoside Rk3, 8: Radix Ginseng
Saponin Rh4, 9: ginsenoside 20 (S)-Rg3, 10: ginsenoside 20 (R)-Rg3, 11: ginsenoside Rk1, 12: ginsenoside
Rg5。
Fig. 2 is the HPLC chromatogram of cooked Radix Notoginseng of embodiment 1 preparation, wherein 1: Panax Notoginseng saponin R1, 2: ginsenoside Rg1, 3:
Ginsenoside Rb1, 4: ginsenoside 20 (S)-Rh1, 5: ginsenoside 20 (R)-Rh1, 6: ginsenoside Rd, 7: ginsenoside
Rk3, 8: ginsenoside Rh4, 9: ginsenoside 20 (S)-Rg3, 10: ginsenoside 20 (R)-Rg3, 11: ginsenoside Rk1, 12: people
Ginseng saponin Rg5。
Detailed description of the invention
Provide present pre-ferred embodiments below in conjunction with the accompanying drawings, to describe technical scheme in detail.
The preparation of embodiment 1 cooked Radix Notoginseng powder
Take raw sangqi ginseng rhizome (clip) dry product 5kg, clean up with drinking water, add 5kg drinking water and be dipped to the heart, with drink
Thoroughly cleaning removing earth impurity with water, be cut into the sheet of 1 millimeter, put in pressure cooker and steam 3 hours in 118 DEG C, 50 DEG C of decompressions are dry
Dry 24 hours, it is ground into fine powder, obtains cooked Radix Notoginseng powder 4.56kg.HPLC method measures the saponin content in cooked Radix Notoginseng powder, and as a result three
Seven saponin R10.38%, ginsenoside Rg11.78%, ginsenoside Rb12.53%, ginsenoside 20 (S)-Rh1
0.63%, ginsenoside 20 (R)-Rh10.34%, ginsenoside Rd 0.56%, ginsenoside Rk30.51%, ginsenoside
Rh41.58%, ginsenoside 20 (S)-Rg30.44%, ginsenoside 20 (R)-Rg30.24%, ginsenoside Rk1
0.26%, ginsenoside Rg51.11%.
The preparation of embodiment 2 cooked Radix Notoginseng sheet
Take raw sangqi ginseng main root dry product 5kg, clean up with drinking water, add 2.5kg drinking water and be dipped to the heart, use drinking water
Thoroughly cleaning and remove earth impurity, be cut into the sheet of 3 millimeters, put in pressure cooker in temperature 116 DEG C, steam 4 hours, 105 DEG C often
Press dry dry 8 hours, obtain cooked Radix Notoginseng sheet 4.42kg.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Panax Notoginseng saponin R1
0.29%, ginsenoside Rg11.40%, ginsenoside Rb11.94%, ginsenoside 20 (S)-Rh10.61%, ginsenoside
20(R)-Rh10.33%, ginsenoside Rd 0.46%, ginsenoside Rk30.50%, ginsenoside Rh41.57%, Radix Ginseng
Saponin 20 (S)-Rg30.42%, ginsenoside 20 (R)-Rg30.22%, ginsenoside Rk10.25%, ginsenoside Rg5
1.16%.
The preparation of embodiment 3 cooked Radix Notoginseng
Taking fresh Radix Notoginseng main root 5kg, clean up, put in pressure cooker in temperature 121 DEG C, steam 2 hours, 100 DEG C of normal pressures are done
Dry 12 hours, obtain cooked Radix Notoginseng 4.67kg.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Panax Notoginseng saponin R1
0.21%, ginsenoside Rg10.78%, ginsenoside Rb12.18%, ginsenoside 20 (S)-Rh10.71%, ginsenoside
20(R)-Rh10.12%, ginsenoside Rd 0.06%, ginsenoside Rk30.39%, ginsenoside Rh41.61%, Radix Ginseng
Saponin 20 (S)-Rg30.17%, ginsenoside 20 (R)-Rg30.08%, ginsenoside Rk10.25%, ginsenoside Rg5
0.65%.
The preparation of embodiment 4 cooked Radix Notoginseng
Take raw sangqi ginseng rhizome dry product 4kg, supporting root dry product 1kg, clean up with drinking water, add 7.5kg drinking water and be dipped to
The heart thoroughly, thoroughly cleans removing earth impurity, puts in pressure cooker and steam 1 hour in 125 DEG C with drinking water, and 80 DEG C of drying under reduced pressure 2 are little
Time, obtain cooked Radix Notoginseng 4.49kg.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Panax Notoginseng saponin R10.47%, Radix Ginseng
Saponin Rg11.93%, ginsenoside Rb12.84%, ginsenoside 20 (S)-Rh10.44%, ginsenoside 20 (R)-Rh1
0.23%, ginsenoside Rd 0.53%, ginsenoside Rk30.41%, ginsenoside Rh40.68%, ginsenoside 20 (S)-
Rg30.37%, ginsenoside 20 (R)-Rg30.23%, ginsenoside Rk10.19%, ginsenoside Rg50.96%.
The preparation of embodiment 5 cooked Radix Notoginseng sheet
Take raw supporting root dry product 5kg, clean up with drinking water, add 5kg drinking water and be dipped to the heart, the most clear with drinking water
Earth impurity is removed in eccysis, is cut into the sheet of 2 millimeters, puts in pressure cooker in temperature 120 DEG C, steams 2.5 hours, 70 DEG C of drying under reduced pressure
16 hours, obtain cooked Radix Notoginseng sheet 4.38kg.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Panax Notoginseng saponin R1
0.15%, ginsenoside Rg10.55%, ginsenoside Rb11.61%, ginsenoside 20 (S)-Rh10.73%, ginsenoside
20(R)-Rh10.41%, ginsenoside Rd 0.49%, ginsenoside Rk30.70%, ginsenoside Rh42.71%, Radix Ginseng
Saponin 20 (S)-Rg30.72%, ginsenoside 20 (R)-Rg30.38%, ginsenoside Rk10.52%, ginsenoside Rg5
1.72%.
The dry Radix Notoginseng of comparative example 1 prepares cooked Radix Notoginseng
According to patent of invention CN 200710066195.X cooked Radix Notoginseng and cooked Panax notoginseng standard extract and its Application Example 1
Method, take dry Radix Notoginseng main root 5kg, be broken for granule, be placed in high-pressure sterilizing pot 120 DEG C and steam 12 hours, do for 55 DEG C
Dry, pulverize, cross 120 mesh, to obtain final product.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Panax Notoginseng saponin R10.04%, people
Ginseng saponin Rg10.12%, ginsenoside Rb10.37%, ginsenoside 20 (S)-Rh10.52%, ginsenoside 20 (R)-Rh1
0.32%, ginsenoside Rd 0.21%, ginsenoside Rk31.28%, ginsenoside Rh42.87%, ginsenoside 20 (S)-
Rg30.24%, ginsenoside 20 (R)-Rg30.12%, ginsenoside Rk10.57%, ginsenoside Rg53.54%.
Comparative example 2 steams the preparation of cooked Radix Notoginseng powder
Preparation method and purposes embodiment 2b cooked Radix Notoginseng according to a patent of invention CN201510023537.4 cooked Radix Notoginseng
The preparation method of powder, takes pseudo-ginseng 5kg, cleans, and cleans, adds 1.75kg drinking water water and run through, with either pure steam in temperature 110
At DEG C, steam 3 hours, dry, pulverize into fine powder, to obtain final product.HPLC method measures the saponin content in cooked Radix Notoginseng powder, result Radix Notoginseng
Saponin R10.63%, ginsenoside Rg13.27%, ginsenoside Rb13.49%, ginsenoside 20 (S)-Rh10.23%,
Ginsenoside 20 (R)-Rh10.10%, ginsenoside Rd 0.47%, ginsenoside Rk30.30%, ginsenoside Rh4
0.87%, ginsenoside 20 (S)-Rg30.21%, ginsenoside 20 (R)-Rg30.11%, ginsenoside Rk10.13%, people
Ginseng saponin Rg50.62%.
The quantitative analysis method of cooked Radix Notoginseng effective ingredient, comprises the following steps:
1) preparation of need testing solution:
Take cooked Radix Notoginseng powder 0.6g, accurately weighed, put in triangular flask, accurate addition methanol 50ml, weighed weight, put 80 DEG C of water
Keep micro-boiling 2 hours in bath, let cool, more weighed weight, supply the weight of less loss with methanol, shake up, filter, take subsequent filtrate, i.e.
?；
2) preparation of reference substance solution:
Precision weighs Panax Notoginseng saponin R respectively1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside 20 (S)-Rh1, Radix Ginseng
Saponin 20 (R)-Rh1, ginsenoside Rd, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20 (S)-Rg3, ginsenoside 20
(R)-Rg3, ginsenoside Rk1, ginsenoside Rg5Reference substance, puts in volumetric flask, adds methanol and is respectively prepared containing about each monomer saponin
The reference substance solution of 3-100 μ g/mL, through 0.45 μm membrane filtration, takes subsequent filtrate and get final product；
3) chromatographic condition
Chromatographic column: Agilent Zorbax SB-C18 (4.6mm × 250mm, 5 μm)；Column temperature: 35 DEG C；Sample size: 20 μ L；
Flow velocity: 1.0mL/min；DAD detector: detection wavelength: 203nm；Flowing phase: water (A)-acetonitrile (B), is carried out by the condition of table 1
Gradient elution: system suitability research requires that the theoretical cam curve of ginsenoside Rb1 is not less than 100000.
Table 1 flows phase gradient
4) methodological study
(1) investigation of standard curve:
Reference substance solution 20 μ L, sample introduction successively, record chromatogram, with peak area (Y), concentration (X) is drawn standard curve,
The results are shown in Table 2.
Result investigated by table 2 each saponin standard solution linear relationship
Result shows, each standard substance are good linear relationship in the range of sample size with peak area.
(2) detection limit, quantitative limit are investigated:
With peak height be about baseline noise 3 times time sample size for detection limit, with entering during peak height about baseline noise 10 times
Sample amount is quantitative limit.The results are shown in Table 3.
Table 3 each saponin detection limit and quantitative limit test data
(3) recovery test
Precision weighs cooked Radix Notoginseng powder 96.00mg 6 parts, puts respectively in 25mL volumetric flask, is separately added into and is equal to sample and contains
Each saponin reference substance of amount, adds 70% methanol aqueous solution to close to scale, the ultrasonic 40min of room temperature, after being cooled to room temperature, and 70% methanol
Aqueous solution constant volume, shakes up, and through 0.45 μm membrane filtration, takes subsequent filtrate, is measured, and calculates the content of each saponin, calculates and reclaims
Rate.The results are shown in Table 4.
Table 4 each saponin response rate investigates result
(4) precision test
Take the mixed mark under linear determination item, ginsenoside Rb1Titer, is repeated in measuring 6 times, and result shows this analysis
Method precision is good.The results are shown in Table 5.
Table 5 each saponin standard solution precision test data
(5) repeated experiment
Precision weighs cooked Radix Notoginseng powder 96.00mg 6 parts, puts respectively in 25mL volumetric flask, adds 70% methanol aqueous solution to connecing
Nearly scale, room temperature supersound extraction 40min, after being cooled to room temperature, 70% methanol aqueous solution constant volume, shake up, extracting solution is filtered through 0.45 μm
Membrane filtration, takes subsequent filtrate, is measured, and calculates the content of each saponin in cooked Radix Notoginseng powder, calculates RSD.Result shows: the method
Repeatability is good.The results are shown in Table 6.
The each saponin content of table 6 cooked Radix Notoginseng extracting solution measures repeated experiment data
(6) stability experiment
Take the sample (96.07mg) under repeated experiment item, within each 2 hours, measure 1 time, investigate 16 hours, each saponin
The RSD of peak area is respectively less than 3.5%, and result shows that in cooked Radix Notoginseng extracting solution, each saponin is basicly stable in 16 hours, is shown in Table 7.
The each saponin content of table 7 cooked Radix Notoginseng extracting solution measures stability experiment data
5) assay
Take need testing solution 20 μ L, sample introduction successively, record chromatogram, calculate for examination according to the standard curve of reference substance solution
The content of various saponin in product solution.
The typical HPLC chromatogram of reference substance and cooked Radix Notoginseng is shown in that Fig. 1 reference substance HPLC chromatogram, Fig. 2 make according to embodiment 1
The HPLC chromatogram of standby cooked Radix Notoginseng.
Claims (8)
1. a cooked Radix Notoginseng, it is characterised in that: based on its percentage by weight meter, the total saponin content of this cooked Radix Notoginseng is 6.0-
12.0%, the rudimentary property saponin being wherein converted into accounts for the 40-90% of cooked Radix Notoginseng total saponins.
Cooked Radix Notoginseng the most according to claim 1, it is characterised in that: containing raw sangqi ginseng intrinsic saponin Panax Notoginseng saponin R1, Radix Ginseng soap
Glycosides Rg1, ginsenoside Rb1Content be followed successively by 0.01-0.5%, 0.2-2.0% and 0.3-3.0%, three's total content be less than 5.0%.
Cooked Radix Notoginseng the most according to claim 1, it is characterised in that: containing the low polarity saponin ginsenoside 20 being converted into
(S)-Rh1, ginsenoside 20(R)-Rh1, ginsenoside Rd, ginsenoside Rk3, ginsenoside Rh4, ginsenoside 20(S)-Rg3、
Ginsenoside 20(R)-Rg3, ginsenoside Rk1, ginsenoside Rg5Content be followed successively by 0.3-1.5%, 0.1-1.0%, 0.05-
1.0%, 0.2-1.5%, 0.5-3.0%, 0.1-1.0%, 0.05-0.5%, 0.1-1.0%, 0.5-3.0%, nine rudimentary property saponin
It is 50-80% that total content accounts for the percentage composition of cooked Radix Notoginseng total saponins.
4. according to the preparation method of cooked Radix Notoginseng described in claim 1 ~ 3 any one, it is characterised in that step is as follows:
1) clean: take Radix Notoginseng dry product, carry out prewashing with water, wash away the earth being attached to Radix Notoginseng surface, add Radix Notoginseng dry product weight 0.5
The drinking water of ~ 2 times amount is dipped to water and blots, then with drinking water Radix Notoginseng carries out fine purifiation, the earth on thorough eccysis Radix Notoginseng surface and
Impurity, must moisten Radix Notoginseng；Or take Radix Notoginseng fresh goods, thoroughly clean earth and the impurity removing Radix Notoginseng surface with drinking water, Radix Notoginseng must be moistened；
2) pretreatment: take profit Radix Notoginseng, dry, pulverize, obtain raw sangqi ginseng powder, adds the water of raw sangqi ginseng grain weight amount 0.5-2 times weight, leaching
Profit, to the saturating heart, obtains profit Radix Notoginseng powder；Or take profit Radix Notoginseng, cut into the sheet of 1-3 millimeter, obtain profit Radix Notoginseng Tabellae；
3) steam: taking profit Radix Notoginseng, profit Radix Notoginseng Tabellae or profit Radix Notoginseng powder, be laid in charging tray, thickness is 1-5 centimetre, puts in pressure cooker,
1-4 hour is steamed in 116-125 DEG C；
4) it is dried: dry 2-24 hour in 50-105 DEG C, obtains cooked Radix Notoginseng, cooked Radix Notoginseng sheet or cooked Radix Notoginseng powder；
5) pulverize: pulverize, obtain cooked Radix Notoginseng powder；Or pulverize, comminution by gas stream, obtain cooked Radix Notoginseng micropowder.
The preparation method of cooked Radix Notoginseng the most according to claim 4, it is characterised in that the Radix Notoginseng dry product or fresh selected in step 1)
Product raw material is one or more in the main root of araliaceae ginseng plant Radix Notoginseng, supporting root, rhizome.
The preparation method of cooked Radix Notoginseng the most according to claim 4, it is characterised in that the Radix Notoginseng dry product or fresh selected in step 1)
Product raw material is the rhizome of araliaceae ginseng plant Radix Notoginseng.
The preparation method of cooked Radix Notoginseng the most according to claim 4, it is characterised in that in step 3) moisten Radix Notoginseng, profit Radix Notoginseng Tabellae,
The condition that steams of profit Radix Notoginseng powder is temperature 118-122 DEG C, 2-3 hour time.
The preparation method of cooked Radix Notoginseng the most according to claim 4, it is characterised in that the drying process in step 4) is preferred
80-100 DEG C dry 6-12 hour.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108042592A (en) * | 2018-02-09 | 2018-05-18 | 云南三七科技有限公司 | A kind of preparation method of ripe notoginseng decoction piece |
| CN108261432A (en) * | 2018-02-26 | 2018-07-10 | 文山苗乡三七科技有限公司 | A kind of Radix Notoginseng preliminary working pruning method |
| CN108938695A (en) * | 2018-08-01 | 2018-12-07 | 安徽金国源中药股份有限公司 | A kind of manufacture craft of ripe Radix Notoginseng powder |
| CN108969549A (en) * | 2018-09-21 | 2018-12-11 | 成都迅达物成生物科技有限公司 | A kind of processing procedure of Radix Notoginseng |
| CN110025646A (en) * | 2019-05-10 | 2019-07-19 | 文山苗乡三七股份有限公司 | Using absolutely dry Radix Notoginseng as the slice process of raw material |
| CN110151806A (en) * | 2018-02-13 | 2019-08-23 | 昆明理工大学 | A kind of ripe Radix Notoginseng and preparation method thereof and purposes of enriching blood |
| CN110151805A (en) * | 2018-02-13 | 2019-08-23 | 昆明理工大学 | A kind of ripe Radix Notoginseng and preparation method thereof and antioxidation application |
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| CN110448612A (en) * | 2019-09-05 | 2019-11-15 | 长春三德天晟科技有限公司 | A kind of intermediate preparation for the Chinese traditional medicines depressing lipid composition drug effect improving the ingredient containing Radix Notoginseng |
| CN111253459A (en) * | 2020-01-19 | 2020-06-09 | 云南大学 | A processed product of notoginsenoside and its preparation method and application |
| CN112315967A (en) * | 2020-09-29 | 2021-02-05 | 吉林瑞诺科技有限公司 | Pseudo-ginseng product |
| CN115531429A (en) * | 2022-10-11 | 2022-12-30 | 昆明理工大学 | Fresh-producing-place processing method for pseudo-ginseng |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485705A (en) * | 2007-09-14 | 2009-07-22 | 中国科学院昆明植物研究所 | Cooked Panax notoginseng and cooked Panax notoginseng standard extract and application thereof |
| CN101502548A (en) * | 2009-03-13 | 2009-08-12 | 广西壮族自治区药物研究所有限公司 | Method for processing cooked notoginseng |
| CN101829170A (en) * | 2010-05-13 | 2010-09-15 | 北京中海康医药科技发展有限公司 | Extract of panax notoginseng saponins and preparation method thereof |
| CN102416028A (en) * | 2011-09-12 | 2012-04-18 | 中国科学院昆明植物研究所 | Preparation method of cooked panax notoginseng extract and total ripe panax notoginseng saponins |
| CN104523790A (en) * | 2015-01-19 | 2015-04-22 | 成都天昕医药保健品有限公司 | Prepared pseudo-ginseng, as well as preparation method and application thereof |
| CN104569209A (en) * | 2015-01-19 | 2015-04-29 | 成都天昕医药保健品有限公司 | Quality detection method of cooked panax notoginseng |
-
2016
- 2016-08-08 CN CN201610644727.2A patent/CN106138141B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485705A (en) * | 2007-09-14 | 2009-07-22 | 中国科学院昆明植物研究所 | Cooked Panax notoginseng and cooked Panax notoginseng standard extract and application thereof |
| CN101502548A (en) * | 2009-03-13 | 2009-08-12 | 广西壮族自治区药物研究所有限公司 | Method for processing cooked notoginseng |
| CN101829170A (en) * | 2010-05-13 | 2010-09-15 | 北京中海康医药科技发展有限公司 | Extract of panax notoginseng saponins and preparation method thereof |
| CN102416028A (en) * | 2011-09-12 | 2012-04-18 | 中国科学院昆明植物研究所 | Preparation method of cooked panax notoginseng extract and total ripe panax notoginseng saponins |
| CN104523790A (en) * | 2015-01-19 | 2015-04-22 | 成都天昕医药保健品有限公司 | Prepared pseudo-ginseng, as well as preparation method and application thereof |
| CN104569209A (en) * | 2015-01-19 | 2015-04-29 | 成都天昕医药保健品有限公司 | Quality detection method of cooked panax notoginseng |
Non-Patent Citations (2)
| Title |
|---|
| 徐维统等: "正交试验法优选熟三七最佳炮制工艺", 《山东中医杂志》 * |
| 武双等: "不同蒸制法对三七主根中皂苷的影响", 《中草药》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110151806A (en) * | 2018-02-13 | 2019-08-23 | 昆明理工大学 | A kind of ripe Radix Notoginseng and preparation method thereof and purposes of enriching blood |
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| CN108969549B (en) * | 2018-09-21 | 2021-04-30 | 天马（安徽）国药科技股份有限公司 | Processing technology of pseudo-ginseng |
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| CN110394217A (en) * | 2019-08-01 | 2019-11-01 | 孙海侠 | A kind of semi-automatic grinding device of the ripe Radix Notoginseng of Chinese medicine based on flow line operation circulation operation |
| CN110394217B (en) * | 2019-08-01 | 2021-05-28 | 河南医学高等专科学校 | Semi-automatic grinding device for cooked traditional Chinese medicine pseudo-ginseng based on flow direction line operation circulation operation |
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| CN111253459A (en) * | 2020-01-19 | 2020-06-09 | 云南大学 | A processed product of notoginsenoside and its preparation method and application |
| CN112315967A (en) * | 2020-09-29 | 2021-02-05 | 吉林瑞诺科技有限公司 | Pseudo-ginseng product |
| CN115531429A (en) * | 2022-10-11 | 2022-12-30 | 昆明理工大学 | Fresh-producing-place processing method for pseudo-ginseng |
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